Establishing maximum daily trunk shrinkage and midday stem water potential reference equations for irrigation scheduling of early maturing peach trees

Measurements of midday stem water potential (Ψ_stem) and maximum daily trunk shrinkage (MDS) were taken over a 4-year period in early maturing peach trees (Prunus persica (L.) Batsch cv. Flordastar) grafted on GF-677 rootstock. Plants were irrigated daily above their water requirements in order to obtain non-limiting soil water conditions. The results indicated that seasonal reference equations can be obtained for MDS and Ψ_stem using crop reference evapotranspiration (ETo), daily mean vapour pressure deficit (VPD_m) and mean daily air temperature (T_m) in the case of MDS, and ETo and VPD_m in the case of Ψ_stem. In this way, VPD_m was seen to be the best predictor of MDS and Ψ_stem, without both were influenced significantly by yield or crop load variations between years. When the postharvest regression between MDS or Ψ_stem and the meteorological parameters mentioned were broken down into early and late postharvest periods, the correlation coefficients improved and were closely related to the presence or absence of sugar-demanding sinks, such as active root growth. A negative linear relationship between MDS and Ψ_stem was found, pointing to unchanging radial hydraulic conductivity in the bark tissues and suggesting that MDS depends to a great extent of the water potential.     

Evaluation of plant-based water status indicators in mature apple trees under field conditions

The performance of different indicators of plant water status as a tool for irrigation management was evaluated in mature field grown ‘Golden Delicious’ apple trees during the late summer of 1998. Control (C) and stress (S) treatments were studied. In the C treatment trees were irrigated daily at 100% ET_c whereas in the S treatment water was withheld during 31 days (DOY’s 236–266). Predawn water potential (Ψ_pd) and midday stem water potential (Ψ_stem) were measured several times a week during the experimental period. Three daily measurements of stomatal conductance (g_s) and stem water potential were made during five consecutive days in mid-September. Trunk diameter changes (TDC) were recorded by LVDT sensors, and from these measurements, maximum daily shrinkage (MDS), daily growth (DG), and cumulative growth (CG) were calculated. Midday Ψ_stem showed the best ratio between the response to moderate water stress and tree variability (“signal/noise” ratio) among the indicators studied here, followed closely by Ψ_pd. On the other hand, the poorest water status indicator was g_s. Due to the low trunk growth rate of the trees, and its high variability, DG and CG were not adequate indicators. MDS showed a lower sensitivity to water stress and a higher variability (CV = 0.19) than midday Ψ_stem (CV = 0.08) and Ψ_pd (CV = 0.10). However, MDS correlated well with ET₀ and with midday Ψ_stem (R ² = 0.79) thus, making this parameter an interesting and promising tool for irrigation management in apple orchards. More research needs to be done in order to define reference values for MDS and plant water potential indicators, in relation to evaporative conditions and in different phenological periods, and to quantify the relationship between water status indicators values and apple tree yield and fruit quality.     

Seasonal sensitivity of stem water potential to vapour pressure deficit in grapevine

Boundary lines of stem water potential (Ψ_stem) responses to vapour pressure deficit (VPD) have been reported for several species and are generally referred to as VPD reference lines (RL). In order to study the response of Ψ_stem to VPD, RLs were determined in plants that received full (Control) and deficit (SSDI) irrigation during three consecutive years. The Control plants received irrigation equivalent to full crop water evapotranspiration minus effective rainfall and the SSDI plants were irrigated at 50% of the Control level. Ψ_stem values for the Control treatment during crop development tended to decrease, and those corresponding to SSDI plants were always lower than those for Control plants. Considering the 3-year data set, no relationship was found between Ψ_stem and VPD. However, there was a differential seasonal response between Ψ_stem and VPD, which depended on the stage of fruit development. A separate phenological analysis enabled the detection of RL for stage II (from fruit-set to veraison) and for stage III (post-veraison), whereas during stage I (from bud-break to fruit set) RL was not apparent. RL slopes increased as the season progressed and were significantly correlated to average values of Ψ_stem. The seasonal decrease in midday Ψ_stem for Control plants was interpreted as being a result of a progressive increase in canopy size and water consumption, which led to increased water depletion before each afternoon’s daily irrigation event. The apparent lack of RL during stage I was related to lower levels of water demand and high Ψ_stem.     

Maximum daily trunk shrinkage and stem water potential reference equations for irrigation scheduling of lemon trees

Measurements of midday stem water potential (ψ_stem) and maximum daily trunk shrinkage (MDS) were done over a 3-year period in adult Fino lemon trees (Citrus limon (L.) Burm. fil.) grafted on sour orange (C. aurantium L.) rootstocks. Plants were irrigated daily above their water requirements in order to obtain non-limiting soil water conditions. The results indicated that reference equations can be obtained for MDS and ψ_stem by pooling data across several seasons using crop reference evapotranspiration (ETo), daily mean vapor pressure deficit (VPD_m) and mean daily air temperature (T _m) in the case of MDS, and ETo in the case of ψ_stem. The best predictor of MDS under non-limiting soil water conditions was T _m, suggesting that MDS reference values can be obtained by means of easy and cheap measurements. MDS and ψ_stem values were not influenced significantly by yield or crop load variations between years. A negative linear relationship between MDS and ψ_stem was found, pointing to an unchanging radial hydraulic conductivity in the bark tissues and suggesting that the MDS is controlled by water potential.     

Evaluation of partial root-zone drying for potential field use as a deficit irrigation technique in commercial vineyards according to two different pipeline layouts

The use of partial root-drying (PRD) irrigation implies doubling pipelines instead of using a conventional single pipeline. However, pipelines can be spaced a short distance apart (e.g. 1 m) along the vine row (“D” layout) or joined with cable ties and laid as a single pipeline (“S” layout). Pipelines in “S” configuration are laid under the vine row, and in “D” at both sides of the vine row. These two different layouts can change the wetted soil zone and affect grapevine response to irrigation. The focus of this study was therefore on establishing the role of pipeline layout in vine-grape (cv. ‘Tempranillo’) response under semi-arid conditions in which PRD is managed as a deficit irrigation technique. Six irrigation treatments were applied, which resulted from the combination of Control (C, full irrigation), PRD and seasonal sustained deficit irrigation (SSDI), and “S” and “D” pipeline layouts. SSDI and PRD were irrigated to 50% C throughout the irrigation season, and C irrigation was scheduled according to a crop water balance technique. Midday stem water potential (Ψ_stem) and leaf conductance (g_l) indicated that, on the whole, PRD treatments had a slightly higher water status than SSDI treatments, but a substantially lower status than C treatments. Use of the “D” pipeline layout significantly reduced Ψ_stem in both PRD and SSDI treatments and in some instances for Control conditions, too. Berry yield, vine intercepted radiation, leaf abscisic acid (ABA) and g_l were highly correlated with Ψ_stem. Differences in water status between PRD-S and SSDI-S, according to a sub-surface irrigation test, seemed to be more related to changes in soil evaporation losses and irrigation efficiency than to any intrinsic PRD effect. PRD-S accounted for water savings equivalent to 10% according to the ratio between applied water and grape production for the SSDI-S treatment, whereas PRD-D berry yield was not significantly different from that associated with the SSDI-S treatment. In conclusion, under the growing conditions of this experiment, PRD-S offered the possibility of slightly improving water conservation when irrigation was applied to the soil surface.     

Evaluation of grapevine water status from trunk diameter variations

We evaluated the usefulness of short-term trunk diameter variations (TDV) as water stress indicator in field-grown grapevines cv. Tempranillo. Two indices were calculated from TDV, maximum daily trunk shrinkage (MDS), and trunk growth rate (TGR). The seasonal evolution of both indicators was compared with occasional determinations of pre-dawn leaf water potential and stem water potential, measured at early morning (Ψ_s^em) and at midday (Ψ_s^md) in irrigated and non-irrigated vines. In the second season, the effect of crop load on the vine water status indicators was also studied. Crop load did not affect either the vine water relations or the TDV. All water potential determinations had much lower variability and were more sensitive than both MDS and TGR to water restrictions. The ability of both indices to detect plant water stress varied largely depending upon the phenological period. In fact, MDS and TGR were only able to detect vine water stress during a short period of time before veraison. During this period, TGR was linearly related to both Ψ_s^em and Ψ_s^md, while for MDS a curvilinear, quadratic equation, better described the relationship with plant water status. After veraison no apparent relationship existed between plant water status and MDS or TGR. Hence, our results question the practical use of both MDS and TGR as variables to automate irrigation scheduling for grapevine.     

Postharvest regulated deficit irrigation in ‘Summit’ sweet cherry: fruit yield and quality in the following season

We examined, over the postharvest seasons of 2005–2007, regulated deficit irrigation (RDI) for its potential of saving water and maintaining fruit yield and quality in ‘Summit’ sweet cherry. The postharvest irrigation treatments were: full irrigation (Control), receiving 80% of water in Control (RDI-80%), and receiving 50% of water in Control (RDI-50%). Midday stem water potential (Ψ_stem) was used for assessing plant water status. In 2006, trees produced a large crop and commercial fruit thinning had to be applied, whereas 2007 was a low crop year. The RDI treatment, first applied in 2005, reduced fruit set in 2006 and also reduced root winter starch concentration. In 2006, fruit set was lower in RDI-50% than in Control. But fruit thinning had still to be done with the final yield being the same among treatments. In 2007, RDI-50% produced more fruit and higher yields than Control. Relationship between postharvest Ψ_stem and crop load in the following season varied according to the year. They were negatively correlated in 2006 and positively correlated in 2007. Fruit firmness did not vary with irrigation treatments in any of the years. Fruit soluble solid concentration (SSC) and fruit relative dry matter (RDM) for RDI-50% was the highest in 2006 when RDI-50% trees had the lowest fruit set. In 2007, SSC and RDM for RDI-50% were the lowest with the trees having the highest fruit set and crop load at harvest. This study indicates that RDI-50% firstly applied in an “off” year, after crop has been harvested, can maintain fruit yield at similar levels to fully irrigated trees while saving water by 45%. Correction of biennial bearing and partial saving of thinning costs are additional advantages of this treatment.     

Influence of crop load on maximum daily trunk shrinkage reference equations for irrigation scheduling of early maturing peach trees

The effects of high crop load (unthinned trees, 22-23 fruits cm⁻² of trunk cross-sectional area (TCSA)), commercial crop load (3-4 fruits cm⁻² of TCSA), and no crop load (all fruitlets removed) on maximum daily trunk shrinkage (MDS), trunk growth rate (TGR) and stem water potential (Ψ_stem) were studied during the fruit growth period and 20 days following harvest in fully irrigated early maturing peach trees, Prunus persica (L.) Batsch, cv. Flordastar. Even though crop load did not affect plant water status, the MDS and TGR values increased and decreased, respectively, as a result of the crop load effect. In this sense, for the same Ψ_stem value, there was a linear increase in MDS with crop load, with a slope of 6.6 μm MPa⁻¹ per unit of crop load increment. The effects of environmental conditions on daily MDS values were also dependent on crop load, suggesting that MDS reference values should be obtained by representing the relations between MDS and the climatic variables (daily mean air temperature, daily mean vapour pressure deficit and daily crop reference evapotranspiration) for a given crop load. The constancy of the relation between MDS and Ψ_stem across crop load underlined the constancy of the elastic properties of the bark tissues.     

Maximum diurnal trunk shrinkage is a sensitive indicator of plant water, stress in Diospyros kaki (Persimmon) trees

Persimmon tree (Diospyros kaki L.f.) is a deciduous fruit tree included in the so-called group of minor fruit tree species. Worldwide, it is not widely grown but, nowadays, Kaki culture is of some importance in the south-east of Spain because of the high fruit commercial value. Currently, neither it is known about Kaki trees water needs, nor crop responses to the irrigation regime. The objective of the present research was to assess the feasibility of using maximum diurnal trunk shrinkage (MDS) as a plant water stress indicator for Kaki trees. During two drought cycles, in trees under either full or deficit irrigation, the MDS obtained by means of LVDT sensors was compared with a reference indicator of fruit trees water status, the midday stem water potential (Ψ_stem). In addition, stomatal conductance and fruit diameter variations were also followed. As water restrictions began, there was an immediate increase in MDS, in correspondence with a decrease in Ψ_stem. Pooling data from both drought cycles and irrigation regimes, MDS and Ψ_stem were linearly correlated (r² = 0.77***). The magnitude of differences between well watered and deficit irrigated trees was much larger in the case of MDS than for Ψ_stem. However, the tree-to-tree variability of the MDS readings was three times higher than for Ψ_stem; average coefficient of variation of 14% and 38% for Ψ_stem and MDS, respectively. Overall, results reported indicated that MDS is a sensitive indicator of Kaki water status and it can be further used as an irrigation scheduling indicator for optimum irrigation management of this crop. However, the large MDS tree-to-tree variability should be taken into account when selecting the number of trees to monitor within an orchard.     

Early morning fluctuations in trunk diameter are highly sensitive to water stress in nectarine trees

The sensitivity to water stress of different plant water status indicators was evaluated during two consecutive years in early nectarine trees grown in a semi-arid region. Measurements were made post-harvest and two irrigation treatments were applied: a control treatment (CTL), irrigated at 120 % of crop evapotranspiration demand to achieve non-limiting water conditions, and a deficit irrigation treatment, that applied around 37 % less water than CTL during late postharvest. The plant water status indicators evaluated were midday stem water potential (Ψ _stem) and indices derived from trunk diameter fluctuations: maximum daily shrinkage (MDS), trunk daily growth rate, early daily shrinkage measured between 0900 and 1200 hours solar time (EDS), and late daily shrinkage that occurred between 1200 hours solar time and the moment that minimum trunk diameter was reached (typically 1600 hours solar time). The most sensitive [highest ratio of signal intensity (SI) to noise] indices to water stress were Ψ _stem and EDS. The SI of EDS was greater than that of Ψ _stem, although with greater variability. EDS was a better index than MDS, with higher SI and similar variability. Although MDS was linearly related to Ψ _stem down to ?1.5 MPa, it decreased thereafter with increasing water stress. In contrast, EDS was linearly related to Ψ _stem, although the slope of the regression decreased as the season progressed, as in the case of MDS. Further studies are needed to determine whether EDS is a sensitive index of water stress in a range of species.     

Determining reference values for stem water potential and maximum daily trunk shrinkage in young apple trees based on plant responses to water deficit

The use of plant water status indicators such as midday stem water potential (Ψ_stem) and maximum daily trunk shrinkage (MDS) in irrigation scheduling requires the definition of a reference or threshold value, beyond which irrigation is necessary. These reference values are generally obtained by comparing the seasonal variation of plant water status with the environmental conditions under non-limiting soil water availability. In the present study an alternative approach is presented based on the plant’s response to water deficit. A drought experiment was carried out on two apple cultivars (Malus domestica Borkh. ‘Mutsu’ and ‘Cox Orange’) in which both indicators (Ψ_stem and MDS) were related to several plant physiological responses. Sap flow rates, maximum net photosynthesis rates and daily radial stem growth (DRSG) (derived from continuous stem diameter variation measurements) were considered in the assessment of the approach. Depending on the chosen plant response in relationship with Ψ_stem or MDS, the obtained reference values varied between −1.04 and −1.46 MPa for Ψ_stem and between 0.17 and 0.28 mm for MDS. In both cultivars, the approach based on maximum photosynthesis rates resulted in less negative Ψ_stem values and smaller MDS values, compared to the approaches with sap flow and daily radial stem growth. In the well-irrigated apple trees, day-to-day variations in midday Ψ_stem and MDS were related to the evaporative demand. These variations were more substantial for MDS than for midday Ψ_stem.     

Wheat basal crop coefficients determined by normalized difference vegetation index

Crop coefficient methodologies are widely used to estimate actual crop evapotranspiration (ET_c) for determining irrigation scheduling. Generalized crop coefficient curves presented in the literature are limited to providing estimates of ET_c for “optimum” crop condition within a field, which often need to be modified for local conditions and cultural practices, as well as adjusted for the variations from normal crop and weather conditions that might occur during a given growing season. Consequently, the uncertainties associated with generalized crop coefficients can result in ET_c estimates that are significantly different from actual ET_c, which could ultimately contribute to poor irrigation water management. Some important crop properties such as percent cover and leaf area index have been modeled with various vegetation indices (VIs), providing a means to quantify real-time crop variations from remotely-sensed VI observations. Limited research has also shown that VIs can be used to estimate the basal crop coefficient (K _cb) for several crops, including corn and cotton. The objective of this research was to develop a model for estimating K _cb values from observations of the normalized difference vegetation index (NDVI) for spring wheat. The K _cb data were derived from back-calculations of the FAO-56 dual crop coefficient procedures using field data obtained during two wheat experiments conducted during 1993–1994 and 1995–1996 in Maricopa, Arizona. The performance of the K _cb model for estimating ET_c was evaluated using data from a third wheat experiment in 1996–1997, also in Maricopa, Arizona. The K _cb was modeled as a function of a normalized quantity for NDVI, using a third-order polynomial regression relationship (r ²=0.90, n=232). The estimated seasonal ET_c for the 1996–1997 season agreed to within −33 mm (−5%) to 18 mm (3%) of measured ET_c. However, the mean absolute percent difference between the estimated and measured daily ET_c varied from 9% to 10%, which was similar to the 10% variation for K _cb that was unexplained by NDVI. The preliminary evaluation suggests that remotely-sensed NDVI observations could provide real-time K _cb estimates for determining the actual wheat ET_c during the growing season.     

Water, nitrogen and yield losses for a nonhomogeneous furrow set

Water distribution can be nonuniform along the furrow length under surface irrigation. This “down field” nonuniformity is combined with “inter-row” non-uniformity which is a consequence of differences in infiltration characteristics across the plot. Global nonuniformity of application depth causes variation of yield, drainage and nitrogen leaching. In addition to that, due to year-to-year variability of climate, irrigation depths range significantly (from 0 to 360 mm/season). The objective of this paper is to study the impact of the nonuniformity of irrigation-water distribution within a furrow plot on yield, water and nitrogen losses when climate variation is taken into account. Six maize vegetation seasons on a Chromic Luvisol soil in the Sofia region with varying irrigation requirements are considered. Irrigation water is distributed in relative terms over the plot at different levels of nonuniformity (coefficient of variation C_v ranging from 13 to 66%) by the FURMOD model. Water and nitrogen cycle and crop growth are simulated then compared at 30 representative points in the set with various “climate-irrigation nonuniformity” combinations by the CERES-maize model. It was established that non-uniformity of irrigation is not important in wet vegetation periods. The drier the irrigation season, the higher the yield loss and risk to environment due to nonuniformity of irrigation water distribution. In moderate and dry irrigation seasons it causes yield losses of 2–14%, significant variation (30% < C_v < 200%) of drainage, nitrogen leaching and residual soil nitrate over the furrow set. Surface irrigation performances can be improved by reducing lateral nonuniformity of stream advance.     

Root growth, water potential and yield of irrigated wheat

Root length density (LV), mid-day leaf water potential (Ψ _leaf) and yield of wheat were studied in 1983 – 1984 and 1984 – 1985 on a Phoolbagh clay loam (Typic Haplaquoll) and on a Beni silty clay loam (Aquic Hapludoll) in the Tarai region of Uttar Pradesh under naturally fluctuating shallow (0.4 – 0.9 m, SWT) and medium-depth (0.8 – 1.3 m, MWT) water table conditions with six water regimes: rainfed (I₀); irrigation at cown root initiation (I₁); at crown root initiation and milk (I₂); at crown root initiation, maximum tillering and milk (I₃); at crown root initiation, maximum tillering, flowering and milk (I₄); and at crown root initiation, maximum tillering, flowering, milk and dough (I₅). Maximum rooting depth (0.8 m under SWT and 1.05 m under MWT conditions) was attained at the dough stage (115 days after sowing, DAS) and was more strongly influenced by fluctuations in water table depth than by the water regime. For wet regimes (I₂– I₅), roots were concentrated at and above the water table interface and had greater horizontal development, whereas in dry regimens (I₀ and I₁), due to deficient moisture conditions in the upper soil layer (0.45 m) they invaded lower horizons and had a greater vertical distribution Ψ _leaf was not significantly affected by water regime (I₁– I₅) up to 94 DAS during a wet year (1983 – 1984) and up to 74 DAS during a dry year (1984 – 1985), but was significantly affected thereafter. Grain yields with water regimens I₁– I₅ during a wet year and for the I₂– I₅ treatments during a dry year at either water table depth were not significantly different, but there was a (non-significant) trend to lower yield with increasing soil water deficit. Under SWT in I₂, the average grain yield wsa 5130 kg ha^–1 and under the I₃ regime, 5200 kg ha^–1. Likewise, under MWT in I₃, it was 5188 kg ha^–1 and under the I₄ regime, 5218 kg ha^–1. The results indicate that application of irrigation of more than 120 and 180 mm under SWT and MWT conditions, respectively, did not raise yield. Irrigation given as per schedule I₂ under SWT and I₃ under MWT conditions in the Tarai situation, appears to be more effective than a very wet regime (I₅). Received: 9 December 1997     

Continuous measurement of plant and soil water status for irrigation scheduling in plum

The usefulness of continuous measurement of soil and plant water status for automated irrigation scheduling was studied in a drip-irrigation experiment on plum (Prunus salicina Black Gold). Two levels of water restriction were imposed at different phenological periods (from pit-hardening to harvest, post-harvest) and compared with a well irrigated control treatment. Soil matrix water potential (_soil) was measured with granular matrix sensors (Watermark); and short-period trunk diameter variation (TDV) was measured with linear variable displacement transformers. The Watermark sensor readings were in reasonable agreement with the irrigation regime and showed a good indication of plant water status across the season (r²=0.62), although they were a better predictor of stem water potential (_stem) in the dry range of _soil. Nonetheless, the most important drawback in their use was the high variability of readings (typical CV of 35–50%). From TDV measurements, maximum daily shrinkage (MDS) and trunk growth rate (TGR) were calculated. Their performance was also compared with _stem, which had the lowest variability (CV of 7%). During most of the fruit growth period, when TGR was minimum, MDS was higher in the less-irrigated treatment than in the control and correlated well (r²=0.89) with _stem. However, after harvest, when TGR was higher, this correlation decreased as the season progressed (r²=0.73–0.52), as did the slope between MDS and _stem, suggesting tissue elasticity changes. Later in the season, TGR was better related to plant water status. These observations indicate some of the difficulties in obtaining reference values useful for irrigation scheduling based exclusively on plant water status measurements.     

Evaluation of crop water stress index for LEPA irrigated corn

This study was designed to evaluate the crop water stress index (CWSI) for low-energy precision application (LEPA) irrigated corn (Zea mays L.) grown on slowly-permeable Pullman clay loam soil (fine, mixed, Torrertic Paleustoll) during the 1992 growing season at Bushland, Tex. The effects of six different irrigation levels (100%, 80%, 60%, 40%, 20%, and 0% replenishment of soil water depleted from the 1.5-m soil profile depth) on corn yields and the resulting CWSI were investigated. Irrigations were applied in 25 mm increments to maintain the soil water in the 100% treatment within 60–80% of the “plant extractable soil water” using LEPA technology, which wets alternate furrows only. The 1992 growing season was slightly wetter than normal. Thus, irrigation water use was less than normal, but the corn dry matter and grain yield were still significantly increased by irrigation. The yield, water use, and water use efficiency of fully irrigated corn were 1.246 kg/m², 786 mm, and 1.34 kg/m³, respectively. CWSI was calculated from measurements of infrared canopy temperatures, ambient air temperatures, and vapor pressure deficit values for the six irrigation levels. A “non-water-stressed baseline” equation for corn was developed using the diurnal infrared canopy temperature measurements as T _c–T _a = 1.06–2.56 VPD, where T _c was the canopy temperature (°C), Ta was the air temperature (°C) and VPD was the vapor pressure deficit (kPa). Trends in CWSI values were consistent with the soil water contents induced by the deficit irrigations. Both the dry matter and grain yields decreased with increased soil water deficit. Minimal yield reductions were observed at a threshold CWSI value of 0.33 or less for corn. The CWSI was useful for evaluating crop water stress in corn and should be a valuable tool to assist irrigation decision making together with soil water measurements and/or evapotranspiration models. Received: 19 May 1998     

Midday measurements of leaf water potential and stomatal conductance are highly correlated with daily water use of Thompson Seedless grapevines

A study was conducted to determine the relationship between midday measurements of vine water status and daily water use of grapevines measured with a weighing lysimeter. Water applications to the vines were terminated on August 24th for 9 days and again on September 14th for 22 days. Daily water use of the vines in the lysimeter (ET_LYS) was approximately 40 L vine⁻¹ (5.3 mm) prior to turning the pump off, and it decreased to 22.3 L vine⁻¹ by September 2nd. Pre-dawn leaf water potential (Ψ_PD) and midday Ψ_l on August 24th were −0.075 and −0.76 MPa, respectively, with midday Ψ_l decreasing to −1.28 MPa on September 2nd. Leaf g _s decreased from ~500 to ~200 mmol m⁻² s⁻¹ during the two dry-down periods. Midday measurements of g _s and Ψ_l were significantly correlated with one another (r = 0.96) and both with ET_LYS/ET_o (r = ~0.9). The decreases in Ψ_l, g _s, and ET_LYS/ET_o in this study were also a linear function of the decrease in volumetric soil water content. The results indicate that even modest water stress can greatly reduce grapevine water use and that short-term measures of vine water status taken at midday are a reflection of daily grapevine water use.     

Assessment of vineyard water status variability by thermal and multispectral imagery using an unmanned aerial vehicle (UAV)

The goal of this study was to assess the water status variability of a commercial rain-fed Tempranillo vineyard (Vitis vinifera L.) by thermal and multispectral imagery using an unmanned aerial vehicle (UAV). The relationships between aerial temperatures or indices derived from the imagery and leaf stomatal conductance (g _s) and stem water potential (Ψ_stem) were determined. Aerial temperature was significantly correlated with g _s (R ²?=?0.68, p?<?0.01) and Ψ_stem (R ²?=?0.50, p?<?0.05). Furthermore, the thermal indices derived from aerial imagery were also strongly correlated with Ψ_stem and g _s. Moreover, different spectral indices were related to vineyard water status, although NDVI (normalized difference vegetation index) and TCARI/OSAVI (ratio between transformed chlorophyll absorption in reflectance and optimized soil-adjusted vegetation index) showed the highest coefficient of determination with Ψ_stem (R ²?=?0.68, p?<?0.05) and g _s (R ²?=?0.84, p?<?0.05), respectively. While the relationship with thermal imagery and water status parameters could be considered as a short-term response, NDVI and TCARI/OSAVI indices were probably reflecting the result of cumulative water deficits, hence a long-term response. In conclusion, thermal and multispectral imagery using an UAV allowed assessing and mapping spatial variability of water status within the vineyard.     

Assessment of trunk diameter variation derived indices as water stress indicators in mature olive trees

Plant age and size, seasonal growth patters and crop load, among other factors, have been reported to decrease the usefulness of trunk diameter variation (TDV) derived indices as water stress indicators in olive trees. Our hypothesis, however, is that indices derived from TDV records in old, big olive trees are sensitive enough to detect levels of water stress in trees of orchards under deficit irrigation that, although severe, are below the threshold for fruit shrivelling. This is of importance for the production of good quality oils, since fruit shrivelling may affect oil quality. The aim of this work was to assess different TDV-derived indices as water stress indicators in 40-year-old ‘Manzanilla’ olive trees with heavy crop load. We derived the maximum daily shrinkage (MDS), daily growth (DG) and daily recovery (DR) from TDV records taken during the 2008 dry season both in well-irrigated FAO trees and in deficit-irrigated RI trees. Measurements of volumetric soil water content (θ_v), leaf water potential (Ψ_l), stomatal conductance (g_s), net CO₂ assimilation rate (A), water and oil accumulation in the fruits and yield parameters were made for both treatments. The trunks did not grow during the experimental season, either in the FAO or RI trees, likely because of the heavy crop load. Therefore, DG was useless as water stress indicator. For MDS and DR, which were responsive to the increase of the trees’ water stress, we calculated the variability, quantified by the coefficient of variation (CV), the signal intensity (S_I) and the sensitivity (S_I/CV) values. In addition, we derived reference equations for irrigation scheduling from the relationships between MDS values in the FAO trees and main meteorological variables. Values both of S_I-MDS and S_I-DR were steady until September 9, despite of increasing differences in θ_v between treatments from early in the dry season. The Ψ_l vs θ_v values showed an outstanding capacity of the RI trees to take up water from the drying soil, and the Ψ_l vs g_s values showed a near-isohydric behaviour of those deficit-irrigated trees. These results explain, at least in part, the lack of response of MDS and DR on that period. Both S_I-MDS and S_I-DR peaked for the first time on September 9, 16 days before the appearance of fruit shrivelling. Our results suggest that using TDV-derived indices as water stress indicators for irrigation scheduling in old olive orchards with medium to low plant densities, i.e. with large root zones, may be useless in case the irrigation strategy is aimed at keeping the soil close to field capacity. Nevertheless, the MDS and DR indices may be useful indicators for the avoidance of fruit shrivelling in deficit irrigated olive orchards for the production of good quality oil. Reliable reference equations for scheduling irrigation with the signal intensity approach were obtained from the regression of MDS values vs the daily maximum values of both the air temperature and the vapour pressure deficit of the air.