Postharvest nitric oxide fumigation delays fruit ripening and alleviates chilling injury during cold storage of Japanese plums (Prunus salicina Lindell)

We investigated the effects of nitric oxide (NO) fumigation on fruit ripening, chilling injury, and quality of Japanese plums cv. ‘Amber Jewel’. Commercially mature fruit were fumigated with 0, 5, 10, and 20 μL L⁻¹ NO gas at 20 °C for 2 h. Post-fumigation, fruit were either allowed to ripen at 21 ± 1 °C or were stored at 0 °C for 5, 6, and 7 weeks followed by ripening for 5 d at 21 ± 1 °C. NO-fumigation, irrespective of concentration applied, significantly (P ≤ 0.5) suppressed respiration and ethylene production rates during ripening at 21 ± 1 °C. At 21 ± 1 °C, the delay in ripening caused by NO-fumigation was evident from the restricted skin colour changes and retarded softening in fumigated fruit. NO treatments (10 and 20 μL L⁻¹) delayed the decrease in titratable acidity (TA) without a significant (P ≤ 0.5) effect on soluble solids concentration (SSC) during ripening. During 5, 6, and 7 weeks of storage at 0 °C, NO-fumigation was effective towards restricting changes in the ripening related parameters, skin colour, firmness, and TA. The individual sugar (fructose, glucose, sucrose, and sorbitol) profiles of NO-fumigated fruit were significantly different from those of non-fumigated fruit after cold storage and ripening at 21 ± 1 °C. CI symptoms, manifest in the form of flesh browning and translucency, were significantly lower in NO-fumigated fruit than in non-fumigated fruit after 5, 6, and 7 weeks storage followed by ripening for 5 d at 21 ± 1 °C. NO-fumigation was effective in reducing decay incidence in plums during ripening without storage and after cold storage at 0 °C for 5, 6, and 7 weeks. In conclusion, the postharvest exposure of ‘Amber Jewel’ plums to NO gas (10 μL L⁻¹) delayed ripening by 3–4 d at 21 ± 1 °C, and also alleviated chilling injury symptoms during cold storage at 0 °C for 6 weeks.     

Quantitative determination of flesh mealiness in peach [Prunus persica L. (Batch.)] through paper absorption of free juice

A simple and rapid method was developed for quantitative determination of juiciness in peach flesh based on the absorption of free juice with ordinary absorbent paper after a flesh sample is squeezed by two metallic rolling cylinders. Juiciness data were compared with trained panel determinations on three peach cultivars kept at 4 °C and 90% RH for 7, 14 and 21 d plus a ripening period at 20 °C and 65% RH until the flesh reached 19.6 ± 9.2 N. There was a high correlation between panel judgment and paper absorption (r² = 0.75 in ‘Elegant Lady’, 0.77 in ‘O’Henry’ and 0.93 in ‘Ross’). A sub-sample of the juiciest and the mealiest fruit also were sorted after 14 and 21 d in cold storage. ‘Ross’, a non-melting peach cultivar, did not develop flesh mealiness during any evaluation period. During storage, there was a reduction in juiciness reaching 15% less after 21 d. Mealy fruit were exclusively observed with melting cultivars exposed to cold storage. The proposed method for determining juice content is easily executed and shows a high association with human perception of juiciness and mealiness in peach.     

Preharvest application of methyl jasmonate (MeJA) in two plum cultivars. 2. Improvement of fruit quality and antioxidant systems during postharvest storage

‘Black Splendor’ (BS) and ‘Royal Rosa’ (RR) plums were treated preharvest with methyl jasmonate (MeJA) at three concentrations (0.5, 1.0 and 2.0 mM) along the on-tree fruit development: 63, 77 and 98 days after full blossom (DAFB). Both control and treated fruit were harvested at the commercial ripening stage and stored in two temperature conditions: 9 days at 20 °C or at 2 °C + 1 day at 20 °C for 50 days. Preharvest MeJA at 2.0 mM significantly accelerated whereas 0.5 mM delayed the postharvest ripening process for both cultivars, since ethylene production, respiration rate and softening were reduced significantly at the two storage conditions for 0.5 mM. In these fruit, total phenolics, total antioxidant activity (hydrophilic fraction, HTAA) and the antioxidant enzymes peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were found at higher levels in treated than control plums during postharvest storage, which could account for the delay of the postharvest ripening process and the extension of shelf-life.     

Role of putrescine in regulating fruit softening and antioxidative enzyme systems in ‘Samar Bahisht Chaunsa’ mango

The role of putrescine (PUT) in regulating fruit softening, antioxidative enzymes and biochemical changes in fruit quality was investigated during ripening and cold storage of mango (Mangifera indica cv. Samar Bahisht Chaunsa). Fruit were treated with various PUT concentrations (0.0, 0.1, 1.0 and 2.0 mM) and were allowed to ripen at 32 ± 2 °C for 7 days, or stored at 11 ± 1 °C for up to 28 days. Respiration rate and ethylene production were measured daily during ripening and cold storage. Cell wall degrading enzymes such as exo-polygalacturonase (exo-PG), endo-polygalacturonase (endo-PG), pectin esterase (PE), endo-1,4-β-d-glucanase (EGase), antioxidative enzymes including superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT), fruit firmness as well as biochemical fruit quality characteristics were estimated during ripening and cold storage at 2 and 7 day intervals, respectively. PUT treatments reduced respiration rate, ethylene production and maintained higher fruit firmness during ripening as well as cold storage. PUT-treated fruit exhibited significantly suppressed activities of cell wall enzymes (exo-, endo-PG and EGase), but retained higher PE activity during ripening and cold storage. Total phenolic and antioxidant contents were significantly higher in PUT-treated fruit during ripening as well in the cold storage period than in the controls. Activities of antioxidative enzymes (CAT, POX and SOD) were also significantly higher in PUT-treated fruit during ripening as well as cold storage. SSC and SSC:TA were lower in PUT-treated fruit, while TA and ascorbic acid content showed the reverse trend. In conclusion, pre-storage 2.0 mM PUT treatment inhibited ethylene production and suppressed the activities of cell wall enzymes, while resulting in higher activities of antioxidative enzymes and maintaining better fruit quality during ripening and cold storage.     

Response of climacteric-type guava (Psidium guajava L.) to postharvest treatment with 1-MCP

Guava (Psidium guajava L. cv. ‘Allahabad Safeda’) fruit harvested at the mature light-green stage were exposed to 300 and 600 nL L⁻¹ 1-methylcyclopropene (1-MCP) for 6, 12 and 24 h at 20 ± 1 °C, and held in either cold storage (10 °C) for 25 days or ambient conditions (25–29 °C) for 9 days. Most of the physiological and biochemical changes during storage and ripening were affected by 1-MCP in a dose dependent manner. Ethylene production and respiratory rates were significantly suppressed during storage as well as ripening under both the storage conditions depending upon 1-MCP concentration and exposure duration. 1-MCP treatment had a pronounced effect on fruit firmness changes during storage under both the conditions. The reduced changes in the soluble solids contents (SSC), titratable acidity (TA) and vitamin C content showed the effectiveness of 1-MCP in retarding fruit ripening. Vitamin C content in 1-MCP-treated fruit was significantly higher than in non-treated fruit, and those treated with 300 nL L⁻¹ 1-MCP for 6 h. The development of chilling injury symptoms was ameliorated to a greater extent in 1-MCP-treated fruit during cold storage and ripening. A significant reduction in the decay incidence of 1-MCP-treated fruit was observed under both the storage conditions. 1-MCP at 600 nL L⁻¹ for 12 h, in combination with cold storage (10 °C) seems a promising way to extend the storage life of guava cv. ‘Allahabad Safeda’ while 1-MCP at 300 nL L⁻¹ for 12 and 24 h or 600 nL L⁻¹ for 6 h, may be used to provide 4–5 days extended marketability of fruit under ambient conditions.     

Low-temperature induction of ripening capacity in ‘Comice’ and ‘Bosc’ pears as influenced by fruit maturity

The relationship between fruit maturity at harvest and the duration of postharvest exposure to ?1 °C required to induce ripening capacity was studied in ‘Comice’ and ‘Bosc’ pears. As fruit of both cultivars were harvested progressively later, shorter durations of exposure to ?1 °C were required to induce ripening capacity. The relationship between the duration of conditioning at ?1 °C and the fruit flesh firmness after 7 d at 20 °C was well-described by second-order polynomial equations. These equations were used to determine the number of days at ?1 °C required to induce ripening capacity for each harvest date. A linear relationship was observed between the number of days after fruit in the orchard reached maturity that fruit were harvested and the number of days of low-temperature conditioning needed to induce ripening capacity. This relationship may be used to predictively estimate the duration of low-temperature conditioning required to induce ripening based on harvest date.     

A novel technique using 1-MCP microbubbles for delaying postharvest ripening of banana fruit

This study aimed to investigate the application of microbubble technology for delaying banana ripening. A preparation of 1-MCP designed for use as a form of aqueous micro bubble (MBs) solutions was formulated. Banana fruit were immersed in 500 nL L⁻¹ of aqueous 1-MCP microbubbles (1-MCP-MBs) or fumigated with 500 nL L⁻¹ 1-MCP, then stored at 25 °C for 8 days. 1-MCP-MBs were more effective in delaying postharvest ripening than conventional 1-MCP fumigation. 1-MCP-MBs reduced the respiration rate and ethylene production compared to the control and 1-MCP fumigated fruit. Moreover, 1-MCP-MBs delayed yellowing and maintained firmness of banana fruit during storage. These results indicate that 1-MCP-MBs can be used as an alternative method for delaying the postharvest ripening of banana fruit, and its application for other commodities needs to be further elucidated.     

Effect of prolonged cold storage on the sensory quality of peach and nectarine

To maintain peach and nectarine quality after harvest, low temperature storage is used. Low temperatures induce physiological disorders in peach, but the effect of cold storage on the sensory quality of the fruit before it is damaged by chilling injury syndrome remains unclear. To evaluate the cold storage effect on the sensory quality two peach cultivars (’Royal Glory’ and ‘Elegant Lady’) and two nectarines (’Ruby Diamond’ and ‘Venus’) were harvested at a standardized firmness level and subjected to quality evaluations and sensory analysis at harvest and after storage at 0 °C for 35 d. For both time points, a supplementary ripening followed such that homogeneous flesh firmness and suitability for consumption was achieved.The fruit segregation through the Durofel firmness (DF), evaluated using a non-destructively method (Durofel device), allowed the formation of a uniform group of fruit in terms of flesh firmness (FF), showing scores between 45.1 and 55.9 N. The average FF in fruit ripened immediately after harvest was 22.9 N and 25.6 N in fruit ripened after cold storage for 35 d.The “acceptability” of fruit is highly correlated with “aroma”, “sweetness”, “juiciness”, “texture” and “flavor”. Only the “acid taste” parameter had no significant correlation with “acceptability” or with the other parameters evaluated.It is possible to conclude that the sensory quality and acceptability of peach and nectarine are characteristic of each cultivar and change, depending on the time elapsed after harvest. In general, it was confirmed that nectarine cultivars have a more consistent quality than peach cultivars.     

Postharvest ethylene conditioning as a tool to reduce quality loss of stored mature sweet oranges

Ethylene is related to senescence but also induces protective mechanisms against stress in plants. The citrus industry only applies the hormone to induce fruit degreening. The aim of this work was to determine the effect of ethylene on the quality of colored citrus fruit stored under commercial conditions to extend postharvest life, since it protects them from stress causing postharvest disorders such as chilling injury (CI) and non-chilling peel pitting (NCPP). The effect of conditioning mature Navelate and Lane Late sweet oranges (Citrus sinensis L. Osbeck) for 4 days with 2 μL L⁻¹ ethylene at 12 °C, rather than at higher temperatures used for degreening, on the quality of fruit stored at 2 or 12 °C, was examined. The ethylene conditioning (EC) treatment did not increase color but reduced calyx abscission and NCPP in fruit of both cultivars stored at 12 °C, and also CI in Navelate fruit at 2 °C. Lane Late fruit did not develop CI but showed a new disorder in EC fruit held at 2 °C. This disorder began as scalded areas around the fruit stem end and extended over the fruit surface during storage. EC had no deleterious effect on the quality of Navelate oranges stored at either 2 or 12 °C. Similar results were found in Lane Late fruit although EC slightly increased off-flavor perception at 2 °C and the maturity index at 2 and 12 °C. Moreover, EC slightly increased the content of bioactive flavonoids in the pulp of Navelate fruit but significant differences between control and EC fruit were only found after prolonged storage at 2 °C. In Lane Late fruit, EC avoided the initial decrease in flavonoid content found in control samples. Results show, therefore, that EC at 12 °C may be a tool to extend postharvest life of NCPP and CI-sensitive oranges, and that the tolerance of citrus cultivars to the combined effect of EC and non-freezing low temperature (2 °C) should be tested to select the proper storage temperature.     

Effect of gum arabic as an edible coating on antioxidant capacity of tomato (Solanum lycopersicum L.) fruit during storage

Coating of tomato fruit with gum arabic has been found to delay the ripening process and maintain the antioxidant capacity. Gum arabic in aqueous solutions of 5, 10, 15 and 20% was applied as an edible coating to green-mature tomatoes which were stored at 20 °C and 80–90% RH for 20 days. Fruit coated with 10% gum arabic delayed the ripening process by slowing down the rate of respiration and ethylene production and also maintained total antioxidant capacity, lycopene content, total phenolics and total carotenoids during storage as compared to the uncoated control and fruit treated with 5% gum arabic concentration. The results suggest that by using 10% gum arabic as an edible coating, the ripening process of tomatoes can be delayed and the antioxidant can be preserved for up to 20 days during storage at 20 °C without any negative effects on postharvest quality.     

Ethylene synthesis,ripening capacity,and superficial scald inhibition in 1-MCP treated ‘d’Anjou’ pears are affected by storage temperature

A continuing challenge for commercializing 1-methylcyclopropene (1-MCP) to extend the storage life and control superficial scald of ‘d’Anjou’ pear (Pyrus communis L.) is how to initiate ripening in 1-MCP treated fruit. ‘D’Anjou’ pears harvested at commercial and late maturity were treated with 1-MCP at 0.15 μL L⁻¹ and stored either at the commercial storage temperature −1.1 °C (1-MCP@−1.1 °C), or at 1.1 °C (1-MCP@1.1 °C) or 2.2 °C (1-MCP@2.2 °C) for 8 months. Control fruit stored at −1.1 °C ripened and developed significant scald within 7 d at 20 °C following 3–5 months of storage. While 1-MCP@−1.1 °C fruit did not develop ripening capacity due to extremely low internal ethylene concentration (IEC) and ethylene production rate for 8 months, 1-MCP@1.1 °C fruit produced significant amounts of IEC during storage and developed ripening capacity with relatively low levels of scald within 7 d at 20 °C following 6–8 months of storage. 1-MCP@2.2 °C fruit lost quality quickly during storage. Compared to the control, the expression of ethylene synthesis (PcACS1, PcACO1) and signal (PcETR1, PcETR2) genes was stable at extremely low levels in 1-MCP@−1.1 °C fruit. In contrast, they increased expression after 4 or 5 months of storage in 1-MCP@1.1 °C fruit. Other genes (PcCTR1, PcACS2, PcACS4 and PcACS5) remained at very low expression regardless of fruit capacity to ripen. A storage temperature of 1.1 °C can facilitate initiation of ripening capacity in 1-MCP treated ‘d’Anjou’ pears with relatively low scald incidence following 6–8 months storage through recovering the expression of certain ethylene synthesis and signal genes.     

Changes in bioactive compounds and response to postharvest storage conditions in purple eggplants as affected by fruit developmental stage

Fruit maturity stage at harvest influences the response to postharvest storage conditions and bioactive compounds content. In this work fruit from two purple eggplant cultivars (Monarca and Perla Negra) were harvested at 12, 15, 18, 20 and 23 d after fruit set (designated as stages I through V) and changes in size, dry weight, calyx area, cell wall material (AIR, alcohol insoluble residue), firmness, respiration, and antioxidants (peel anthocyanins and pulp carotenoids, ascorbic acid, phenolics and chlorogenic acid) were determined. In a second set of experiments the postharvest performance of fruit harvested at stages I (“baby” eggplants), III and IV (traditional harvest stages) during storage at 0 or 10 °C was assessed. Fruit growth continued until late ripening in contrast to calyx expansion and peel anthocyanin accumulation, which were relatively earlier events. Fruit dry weight decreased between stages I and III, remaining constant afterwards. “Baby” eggplants had higher antioxidant capacity, chlorogenic acid (ChA), carotenoids and ascorbic acid contents than late-harvested fruit. ChA predominated in pulp placental tissues at stage I, spreading throughout the fruit core at as ripening progressed. No marked differences in dry mass, antioxidant capacity or responses to postharvest storage regimes were found between fruit harvested at stages III and IV. Late pickings increased yields and led to less dense fruit, which had lower respiration rates. Within this harvest window, storage at 10 °C maximized quality maintenance. In contrast “baby” eggplants stored better at 0 °C. Understanding the developmental changes in bioactive compounds and postharvest performance may help in the maximization of fruit antioxidant properties as well as in the selection of the optimal handling conditions for each ontogenic stage.     

Effect of oxalic acid on ripening attributes of banana fruit during storage

The effect of exogenous oxalic acid treatment on ripening attributes of banana fruit during storage was investigated. Banana fruit were dipped into solutions of 0 (control) or 20 mM oxalic acid for 10 min and then stored at room temperature (23 ± 2 °C) and 75–90% relative humidity. The application of oxalic acid reduced fruit deterioration during storage. The oxalic acid treatment also reduced the rates of respiration and ethylene production, and delayed the decreases in firmness, hue angle, and maximal chlorophyll fluorescence (Fv/Fm) of banana fruit during storage. Furthermore, fruit treated with oxalic acid exhibited higher superoxide dismutase activity and antioxidant capability with a lower production of reactive oxygen species at the late storage period compared with non-oxalic acid-treated fruit. Overall, the oxalic acid treatment was effective in inhibiting postharvest ripening of banana fruit and exhibited the potential for commercial application to store the bananas at room temperature. It can be concluded that the delay in banana fruit ripening associated with oxalic acid treatment could be due to inhibition of respiration and ethylene production rates, and reduction of oxidative injury caused by reactive oxygen species through increased antioxidant activity.     

Effectiveness of submicron chitosan dispersions in controlling anthracnose and maintaining quality of dragon fruit

Conventional chitosan (CC) and submicron chitosan dispersions (SCD) were evaluated for the control of postharvest anthracnose and maintenance of quality of dragon fruit during storage at 10 ± 2 °C and 80 ± 5% RH for 28 days. All the chitosan treatments significantly reduced anthracnose symptoms, resulting in a reduction of disease development and thereby maintained the quality of fresh fruit for extended periods. SCD at 1.0% with 600 nm droplet size gave the best result in that it delayed the onset of disease and maintained the quality of dragon fruit for up to 28 days of storage. It can be concluded from the present investigation that SCD have potential to be used as an antifungal agent to control postharvest anthracnose and maintain quality of dragon fruit during storage.     

Physiological response of ‘Larry Ann’ plums to cold storage and 1-MCP treatment

The aim of this work was to study the specific effects of low temperature and 1-MCP treatment on ethylene metabolism and oxidative behaviour in plums (Prunus × salicina cv. Larry Ann). Control fruit were stored at 20 °C or 0 °C and the 1-MCP (625 nL L^?1) treated fruit at 0 °C. Changes in the kinetics of ethylene production upon removal were related to changes in ACC metabolism (ACC and MACC levels), oxidative behaviour (H₂O₂ content) and enzymatic antioxidant potential (SOD, CAT and POX enzymes) during cold storage. Low temperature stress inhibited the synthesis of MACC, which appeared to be the basic process that regulated ACC and ethylene production at ambient temperature. Although 1-MCP treatment inhibited ethylene production and ACC accumulation in the cold, it did not inhibit the accumulation of MACC. Neither cold nor 1-MCP treatment induced oxidative stress. Nevertheless, the 1-MCP treatment significantly impaired the increase in POX activity observed during cold storage. Collectively these results showed the underlying role that ACC metabolism plays in the ripening behaviour of cold-stored plums, confirming previous results. The results also indicate that MACC and malonyl transferase activity are the key regulatory factors that control ripening and possibly some ethylene-related disorders such as chilling injury in cold-stored plums.     

Use of equilibrium modified atmosphere packaging for preservation of ‘San Antonio’ and ‘Banane’ breba crops (Ficus carica L.)

The purpose of this work was to study the effect of equilibrium modified atmosphere packaging on the stability of ‘San Antonio’ and ‘Banane’ breba fruit during postharvest cold storage by the use of three different microperforated films (1/50 mm, 1/30 mm, and 1/10 mm; ø = 100 μm). Gas composition in the wraps, weight loss, % disorder, and microbial counts were monitored during cold storage for 21 days. The tested microperforated films allowed the extension of cold storage time for brebas, minimizing weight loss and delaying the disorders due to fungal proliferation, especially M50 (1/50 mm). Total soluble solids (TSS), titratable acidity (TA), pH, firmness, and sensory quality were also evaluated. Among the tested microperforated films, M50 showed the best performance in terms of delaying physicochemical senescence processes of fruit. The breba cultivar had an important impact on the extension of cold storage. For ‘San Antonio’ and ‘Banane’ cultivars packaged with microperforated M50, the optimal time of cold storage was 14 and 21 days, respectively.     

Effects of cold storage and shelf-life on sensory quality and consumer acceptance of ‘Abate Fetel’ pears

Fruit products certified by quality labels should guarantee high levels of consumer acceptance, despite the unavoidable variability arising from growing conditions and postharvest responses. The quality of ‘Abate Fetel’ pear (Pyrus communis L.) fruit was studied, after short or long cold storage, by analysis of physicochemical, texture and flavour traits, to investigate factors affecting consumer acceptance. Fruit from three orchards differing in location and design, monitored during 10 d of ripening at 20 °C, softened progressively to reach and exceed firmness adequate for consumption. Change in colour, in particular hue angle, paralleled softening. Sensory traits were investigated by evaluating fruit of three different firmness levels within the range of acceptable eating quality. Firmness differences were clearly perceived both by expert judges and by consumers, but did not influence the degree of liking. ‘Abate Fetel’ pear can maintain acceptable eating quality at 20 °C for 4–8 d after 13 weeks storage at ?1 °C, or 2–6 d after 23 weeks storage at ?1 °C. Changing texture parameters were perceived at eating, without compromising overall quality. Production system affected intrinsic quality parameters such as total soluble solids concentration, but did not influence consumer acceptance. In consumer tests conducted after 13 weeks of cold storage, high scores were recorded, with a 86% acceptance frequency and more than 40% of scores reflecting “like very much” or “like extremely”. After 23 weeks of cold storage a decrease in degree of liking was observed. The overall value of ‘Abate Fetel IGP Emilia-Romagna’ quality label was confirmed by consumer evaluations. However, the decrease in consumer acceptance after 23 weeks of cold storage indicates that caution should be used in using long storage durations.