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1.
转基因水稻秸秆还田对土壤硝化反硝化微生物群落的影响   总被引:2,自引:0,他引:2  
转基因作物可能通过根系分泌物和植株残体组成的改变及外源基因的转移释放令土壤微生物群落产生变化,影响土壤微生物的生态功能。氨氧化细菌和反硝化细菌是驱动土壤硝化和反硝化过程的关键微生物,其群落结构的变化直接关系土壤氮素的转化与利用。本研究利用荧光定量PCR和PCR-DGGE技术分析了转cry1Ac/cpti双价抗虫基因水稻‘Kf8’秸秆还田降解过程中,土壤氨氧化细菌和反硝化细菌群落丰度与组成的变化,探讨转基因水稻是否存在影响稻田土壤氮素转化与N2O排放的可能。结果显示:无论是氨氧化细菌amo A基因还是反硝化细菌nirS基因,其丰度在转基因水稻‘Kf8’与非转基因水稻‘Mh86’的秸秆还田土壤中都没有显著差异;转基因水稻‘Kf8’和非转基因水稻‘Mh86’秸秆还田降解过程中0~10 cm土层中的amo A基因丰度均显著高于10~20 cm及20~30 cm土层(P0.05);各深度土层中的nirS基因丰度均存在随秸秆还田时间延长而增加的趋势。水稻秸秆还田降解过程中,转基因水稻‘Kf8’的土壤氨氧化细菌和反硝化细菌的群落多样性指数及组成,均与非转基因水稻‘Mh86’没有显著差异。相关分析结果表明土壤氨氧化细菌和反硝化细菌群落组成均与水稻秸秆还田时间存在显著相关性(P=0.002),反硝化细菌群落组成还与土层深度显著相关(P=0.024)。本研究表明转cry1Ac/cpti抗虫基因水稻秸秆还田对稻田土壤硝化和反硝化关键微生物群落不会产生明显影响。就土壤微生物群落而言,转cry1Ac/cpti抗虫基因水稻秸秆还田不存在影响土壤氮素转化与N2O排放的可能。  相似文献   

2.
三江平原是黑龙江省水稻主要种植区,白浆土是主要种稻土壤,在白浆土上开展秸秆还田试验研究,明确白浆土秸秆还田效果,根据土壤肥力水平调控氮素为秸秆还田提出因地制宜的土壤、施肥等管理技术提供参考。该文以白浆土为供试土壤,比较研究不同肥力白浆土上连续秸秆还田及调控氮素对水稻产量及土壤养分变化的影响。结果得出:高肥力土壤连续秸秆还田适合减氮,减氮10%连续3年水稻不减产,增产幅度为0.1%~6.94%,减氮20%以上产量降低,秸秆连续还田增加氮素水稻产量第1年与正常施肥比增产4.47%,第2年水稻产量比对照减产4.02%~31.86%,调氮降低幅度大;中、低肥力土壤秸秆还田水稻产量第1年比对照分别增加1.48%,4.52%,第2年调氮增产幅度会下降;秸秆还田使土壤有机质、氮素含量提高,在高肥力土壤上氮素过高使水稻前期分蘖量增多,水稻有效穗数降低,产量降低,减氮后可以避免土壤氮素过剩,水稻产量提高;中、低肥力土壤秸秆还田有利于增加土壤肥力水平,适当增加氮素可使水稻产量提高。  相似文献   

3.
土壤酶活性对大田单季种植转Bt基因及转双价棉花的响应   总被引:6,自引:0,他引:6  
利用田间试验,以转Bt基因棉花Z30、转双价(Bt+CpTI)棉花SGK321及其相应的等价基因Z16、SY321作为供试对象,研究棉花种植后对土壤水解/氧化还原酶类活性的影响。结果表明,转Bt棉花及转双价棉花的种植对各种土壤酶活性的影响不一致,转Bt棉花及转双价棉花种植显著降低了土壤蛋白酶和脱氢酶活性(P 0.05)。转Bt棉花还显著降低了土壤脲酶活性(P 0.05);转双价棉花种植同时显著降低土壤磷酸二酯酶、β-葡萄糖苷酶、过氧化氢酶和硝酸还原酶活性(P 0.05)。棉花品种及转基因行为(转入基因类型)均未给土壤酸性磷酸单酯酶和FDA活性带来影响;土壤芳基硫酸酯酶所受的影响主要来自于棉花品种自身。  相似文献   

4.
为了探究控释尿素掺混肥与玉米秸秆长期互作对麦季土壤酶活性和土壤养分状况的影响,基于华北平原棕壤区小麦—玉米轮作8年定位施肥试验,对比研究了玉米秸秆还田(S)与不还田条件下施用控释尿素掺混肥(CRF)与普通尿素掺混肥(BBF)对麦季土壤酶活性及土壤养分的影响。结果表明:秸秆不还田条件下,CRF处理较BBF处理显著提高土壤中性磷酸酶(返青期和成熟期)、蔗糖酶(返青期和孕穗期)和纤维素酶(返青期和孕穗期)活性及成熟期有机质、硝态氮和有效磷含量,其中,成熟期中性磷酸酶活性显著提高29.6%,硝态氮含量显著提高34.8%。秸秆还田条件下,BBF+S处理成熟期脲酶、纤维素酶,孕穗期中性磷酸酶活性显著高于CRF+S处理,其他时期2种类型掺混肥土壤酶活性无显著差异。CRF+S处理成熟期土壤全氮、有机质、硝态氮和有效磷含量显著高于BBF+S处理。氮肥种类和玉米秸秆还田的交互作用对土壤中性磷酸酶、蔗糖酶和纤维素酶活性及土壤全氮、硝态氮、铵态氮、有效磷和速效钾含量影响显著,秸秆还田较不还田处理显著提高中性磷酸酶、蔗糖酶活性、速效钾和有效磷含量。综上表明,玉米秸秆还田和不还田条件下控释尿素掺混肥较普通尿素掺混肥处理均能显著增加土壤有机质含量,提高小麦后期速效氮磷养分的供应强度和供应容量,其中玉米秸秆还田配施控释尿素掺混肥处理在提高土壤酶活性和土壤养分方面表现最优。研究结果可为秸秆还田下氮肥优化施用提供理论依据。  相似文献   

5.
长期减量化施肥对水稻产量和土壤肥力的影响   总被引:6,自引:0,他引:6  
为探讨减量化施肥对水稻产量及土壤肥力的影响,对巢湖流域连续10年减量化施肥和秸秆还田定位试验后的水稻产量、土壤有机质、有效氮磷钾、有效铜锌铁锰含量及土壤酶活性进行了测定和分析。结果表明:长期减氮30%或减磷50%处理对水稻产量无明显降低作用,减量+秸秆还田有增产作用,但增产不显著;减氮30%或减磷50%会降低土壤中有机质含量,增加土壤中速效钾、碱解氮含量,减磷50%土壤中的有效磷含量显著降低,减量+秸秆还田会增加土壤中的有机质、速效钾、碱解氮、有效铜锌铁锰含量,对有效磷的增加效果不显著;较不施肥相比,施肥能够明显提高土壤的酶活性,减少氮磷肥会一定程度地降低土壤中的酶活性,减量+秸秆还田对脲酶的增加效果不明显,但会显著增加土壤中性磷酸酶和蔗糖酶活性;土壤酶活性与水稻产量及土壤养分含量之间呈显著或极显著正相关关系。综合考虑减量化施肥对水稻产量和土壤肥力的影响,可提出在巢湖流域实施减量化施肥+秸秆还田处理来代替高产施肥。  相似文献   

6.
设施土壤长期存在碳输入不足的问题,秸秆颗粒是实现外源碳大量输入的重要途径之一。采用盆栽试验方式,设置粉碎秸秆、秸秆颗粒两种秸秆类型,4种秸秆还田量水平(干土重的1%、2%、3%、4%),以无秸秆还田为对照(CK),研究秸秆颗粒不同量还田对设施土壤碳氮循环相关酶活性的影响。结果表明:(1)秸秆颗粒与粉碎秸秆还田均对纤维素酶、β-葡糖苷酶、α-葡糖苷酶、脲酶、亮氨酸氨基肽酶、乙酰氨基葡萄糖苷酶、谷氨酰胺合成酶、酸性磷酸酶的活性有正效应,但对酚氧化酶活性具有负效应。对过氧化氢酶活性表现为少量秸秆还田时是正效应,高量秸秆还田时是负效应;(2)秸秆颗粒与粉碎秸秆处理的土壤酶活性均随还田量的增加呈先增加后降低变化,秸秆颗粒与粉碎秸秆处理的差异主要与还田量水平相关。还田量超过2%达到一定水平后,对酶活性的影响均出现正效应降低的效果。高量还田条件下在3%还田量水平以上时,秸秆颗粒更利于改善土壤碳氮循环相关酶活性;(3)秸秆颗粒大量还田可显著增加土壤养分含量;(4)土壤养分含量与土壤酶活性显著相关,是土壤酶活性变化的主控因子;(5)秸秆颗粒的适宜还田量为3%~4%,而粉碎秸秆的适宜还田量不宜超过2%。总...  相似文献   

7.
稻麦两熟制不同耕作方式与秸秆还田土壤肥力的综合评价   总被引:18,自引:6,他引:12  
该文通过大田小区和网室水泥池微区3年的定位试验,比较研究了稻麦两熟条件下免耕套播秸秆覆盖NTS、免耕套播高茬NT、翻耕秸秆还田CTS、翻耕秸秆不还田CK共4个处理对土壤肥力的影响,并运用数值化方法综合评价土壤肥力.结果表明:免耕和秸秆还田可提高土壤有机质、速效磷、速效钾等土壤养分含量,且主要是0~7cm增加造成的.土壤肥力数值化综合评价表明,土壤肥力免耕秸秆覆盖好于免耕高茬,翻耕秸秆还田好于翻耕不还田,不同处理养分肥力指标以免耕秸秆覆盖处理最高,翻耕不还田最低;但综合肥力指标却以翻耕秸秆还田最高,免耕高茬最低,主要受土壤容重影响.从不同层次看,综合肥力指标和养分肥力指标均以上层O~7cm较高,下层14~21cm较低,尤其免耕秸秆覆盖和免耕高茬处理.  相似文献   

8.
为寻求适宜当地生产和生态环境的最适耕作措施和秸秆还田方式组合,通过开展2年(2009-2011年)田间试验,研究不同耕作措施和秸秆还田对稻麦轮作农田土壤养分、微生物量碳氮及土壤酶活性的影响.结果表明:无论是翻耕还是旋耕,秸秆还田条件下的土壤养分含量均不同程度地高于秸秆不还田,除速效钾外,差异均达显著水平;两季秸秆均还田处理土壤微生物量碳含量均显著高于两季秸秆均不还田;除旋耕秸秆两季均还田外,旋耕麦季稻秸还田处理土壤微生物量氮含量显著高于其他各处理;与翻耕秸秆不还田相比,翻耕两季秸秆均还田和旋耕两季秸秆均还田均显著提高了土壤脲酶和蔗糖酶活性,其中脲酶提高了10.96%和9.72%,蔗糖酶提高了30.36%和17.87%.  相似文献   

9.
为研究转Chi+Glu双价基因棉对土壤微生物群落功能多样性的影响,揭示其对土壤生态系统的安全性,在大田试验条件下,利用Biolog代谢指纹方法分析了种植转Chi+Glu双价基因棉、转Bt基因棉和常规棉不同生育期土壤微生物群落功能多样性。结果表明,两个转基因棉花土壤微生物数量差异不显著,而转基因棉花土壤细菌和放线菌数量在花期和铃期显著高于常规棉花(P〈0.05),真菌数量在花期和铃期却显著低于常规棉花(P〈0.05)。与非转基因棉花相比,转基因棉花土壤微生物群落碳源利用能力在花期和铃期显著增加,转基因棉花土壤微生物群落的Shannon指数和McIntosh指数在花期、铃期和吐絮期显著高于常规棉花,Simpson指数在花期和铃期则显著低于常规棉花。主成分分析结果显示,花期转基因棉花和常规棉花对31种碳源的利用差异较大。转基因棉花在苗期和蕾期对羧酸类、氨基酸类和多胺类,花期和铃期对糖类、羧酸类和双亲化合物类的利用分别较常规棉高;吐絮期转基因和常规棉花对所有碳源的利用均较低,其中转基因棉花对糖类和羧酸类的利用高于常规棉花。研究结果显示,种植转Chi+Glu双价基因棉花在花期和铃期对土壤微生物群落影响显著,其与转Bt基因棉花无明显差异。  相似文献   

10.
通过田间定位试验,研究了稻草秸秆还田对土壤有机质及其养分含量、土壤微生物生物量、土壤酶活性及水稻产量的影响。试验结果表明:秸秆还田显著提高了土壤有机质及养分含量,与对照相比,秸秆还田的处理土壤有机质含量提高了7.00%,土壤全氮、速效磷、速效钾和缓效钾含量分别提高了3.57%、3.49%、4.11%和3.39%;秸秆还田显著提高了土壤微生物生物量碳、氮,与对照相比,土壤微生物量碳、氮分别提高了37.4%和21.8%;秸秆还田显著提高了土壤酶活性,与对照相比,土壤脲酶、过氧化氢酶和蔗糖酶的活性分别提高了67.4%、12.4%和7.43%;秸秆还田显著提高了水稻产量,与对照相比,秸秆还田的处理和秸秆还田并配施腐熟剂的处理水稻实际产量分别增加了4.85%和5.96%。  相似文献   

11.
In order to identify key adaptive traits which affect productivity in Mediterranean grain and forage legumes and simultaneously determine the agricultural potential of a wide range of Vicia species, germplasm collected from the wild throughout the eastern Mediterranean was grown under semi-arid conditions in Tel Hadya, Syria (313 mm growing season rainfall). These included species currently in use in Mediterranean agriculture, such as V. sativa L., as well as those more widely used in the past-such as V. ervilia L., but also a broad selection from Section Narbonensis (B. Fedtsch. ex Radzhi) Maxted, including V. narbonensis L, V. johannis Tamamsch., V. hyaeniscyamus Mout., V. serratifolia Jacq., and V. kalakhensis Khattab et al. V. faba, a near relative of the taxa in Section Narbonensis, was included as a domesticated control. Where applicable, a representative range of subspecies was used. Accessions were chosen from a wide range of habitats in terms of latitude (31.02–40.72 decimalo), longitude (27.1–43.17 decimalo), altitude (20–1510 m), rainfall (180–1700 mm/yr) and soil depth (5–50 cm) in order to maximise diversity within species. Agricultural potential was determined by measuring seed, hay and biological yield, as well as agronomic traits such as harvest index, standing crop height, and seed size. The comparative influence of phenology and key agronomic traits such as plant habit and seed size on productivity varied tremendously between species, depending on their reproductive strategies. In V. sativa and V. ervilia, the smaller seed species which rely on long vegetative phases and growing seasons to accumulate sufficient biomass to set seed, and in which there was comparatively little agronomic variation, phenology had a large impact on yield. In early emerging taxa such as V. ervilia and V. s. subsp. sativa, with built-in long vegetative phases and growing seasons, seed yield was negatively correlated with flower ing (r = –0.86 to –0.88), whereas the opposite was the case for later emerging taxa such as V. s. subsp. nigra (L.) Ehrh. (r = 0.95). Within V. narbonensis and relatives, the larger seeded Vicia species which rely on more conservative reproductive strategies where high seedling vigour associated with large seeds enables the species to enter reproductive phases relatively early, phenology had a much smaller impact on yield than did variation of key traits such as seed weight, plant habit and pod shattering. Among the undomesticated germplasm harvest indices ranged from 0.09–0.31, hay yields from 0.1–3.4 t/ha, seed yield from 0–2.0 t/ha, and dry matter at maturity from 1.6–6.5 t/ha. Sub-specific taxonomy was crucial in determining agronomic potential. V. narbonensis var. aegyptiaca Kornhuber ex Asch. et Schweinf. showed the most potential, combining an upright habit, large seeds (212 mg) and tendency to retain intact pods after maturity, with the highest yield, harvest index and crop height of all the wild Vicia species. V. sativa subsp. sativa, V. ervilia and V. narbonensis var. narbonensis were less productive, but still showed agricultural potential. The smaller seeded V. narbonensis, var. affinis, var. jordanica H. Schäf. and var. salmonea (Mout.) H. Schäf., and their close relatives V. johannis, V. hyaeniscyamus, V. serratifolia and V. kalakhensis have little to offer Mediterranean agriculture on the basis of poor agronomy.  相似文献   

12.
The effects of bacterial inoculants on the growth of winter wheat were studied in a growth chamber. Azospirillum brasilense, Azotobacter chroococcum, Bacillus polymyxa, Enterobacter cloacae, or a mixture of the four rhizobacteria were the inoculants tested. Inoculation effects on yield, yield components, and N-derived from fertilizer (Ndff) were assessed. The response of plants inoculated with individual bacteria was inconsistent and varied with treatment. At the first harvest (58 days after planting-DAP) plants inoculated with the mixture exhibited increases in plant dry weight, total-N and Ndff. At the second harvest (105 DAP), plants inoculated with A. brasilense and the mixture exhibited increases in shoot biomass, whereas at maturity (170 DAP), the inoculated plants showed no differences in total-N or shoot dry matter yield, as compared to the uninoculated controls. Inoculation with A. brasilense, however, increased the Ndff in the shoots, and B. polymyxa tended to enhance grain yield. Practical use of these rhizobacteria as inoculants for winter wheat may have limited value until such time as we better understand factors which influence rhizosphere competence of bacterial inoculants.  相似文献   

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The powerful mass-flow of melt water during the brief period of spring thaw provides a surge of pollutants and causes extremely toxic short term conditions. Together with the increase in total metal concentrations during this period, a redistribution in metal speciation occurs that increases the proportion of more toxic forms. Concentrations of ion-exchangeable fractions of nickel (Ni), copper (Cu), aluminium (Al), and manganese (Mn) show strong negative correlations with pH. The sum of complex metal species shows a strong positive correlation with total organic carbon (TOC). A data analysis model based on relationships observed between key parameters and metal behaviour together with gidrological data allowed an assessmrnt of the metal impact on waterbodies from accumulated airborne pollution (Ni, Cu) as well as from accelerated leaching from watersheds (Al, Mn).  相似文献   

16.
The difficulty and problems encountered in the study of cultivated plants, in general and sugarcane, in particular has been indicated. In order to understand these problems, a brief review on the taxonomy of Saccharum and closely related taxa, namely, Erianthus, Sclerostachya, Narenga and Miscanthus (generally known as ‘Saccharum complex’) has been given. A short account on the important morphological features that are specific to sugarcane has also been stated as classification is commonly based on such morphological characters. A note has been added on the chromosome number, origin, and distribution of the species of ‘Saccharum complex’ members. Taxonomic keys have been devised for identification of the genera of Saccharinae and for the species of Saccharum and Erianthus occurring in India. A new combination, Sclerostachya fallax (Balansa) Amalraj et Balasundaram, has also been proposed.  相似文献   

17.
We investigated the diversity of rhizobia isolated from different indigenous legumes in Flanders (Belgium). A total of 3810 bacterial strains were analysed originating from 43 plant species. Based on rep-PCR clustering, 16S rRNA gene and recA gene sequence analysis, these isolates belonged to Bradyrhizobium, Ensifer (Sinorhizobium), Mesorhizobium and Rhizobium. Of the genera encountered, Rhizobium was the most abundant (62%) and especially the species Rhizobiumleguminosarum, followed by Ensifer (19%), Bradyrhizobium (14%) and finally Mesorhizobium (5%). For two rep-clusters only low similarity values with other genera were found for both the 16S rRNA and recA genes, suggesting that these may represent a new genus with close relationship to Rhodopseudomonas and Bradyrhizobium. Primers for the symbiotic genes nodC and nifH were optimized and a phylogenetic sequence analysis revealed the presence of different symbiovars including genistearum, glycinearum, loti, meliloti, officinalis, trifolii and viciae. Moreover, three new nodC types were assigned to strains originating from Ononis, Robinia and Wisteria, respectively. Discriminant and MANOVA analysis confirmed the correlation of symbiosis genes with certain bacterial genera and less with the host plant. Multiple symbiovars can be present within the same host plant, suggesting the promiscuity of these plants. Moreover, the ecoregion did not contribute to the separation of the bacterial endosymbionts. Our results reveal a large diversity of rhizobia associated with indigenous legumes in Flanders. Most of the legumes harboured more than one rhizobial endosymbiont in their root nodules indicating the importance of including sufficient isolates per plant in diversity studies.  相似文献   

18.
Summary Pot-culture studies were carried out to examine the response of barley (Hordeum vulgare L.) to inoculation with Azospirillum brasilense and Glomus versiforme, singly and/or in combination, under varying levels of nitrogenous [(15NH4)2SO4] and soluble phosphatic (single superphosphate) fertilizers. The interaction between both the endophytes led to increased growth and nutrition of the barley plants. Roots from plants inoculated with Azospirillum brasilense and Glomus versiforme exhibited very low acetylene reduction activity. N2 fixation in the plants increased with the increase in plant growth but the mycorrhiza alone gave a low level of N2 fixation in the plants compared to combined inoculation with both the endophytes.  相似文献   

19.
Gorse (Ulex europaeus) infestation occupies over 4,000 ha of agriculture and conservation lands on the southeastern slope of Mauna Kea on the Island of Hawaii. The aim of this investigation is to identify ecological features associated with this weed invasion by comparing the gorse-infested areas to the surrounding uninfested areas of this landscape. The soils within the gorse infestation are more acidic, resulting in higher levels of KCl-extractable Al and lower levels of Mehlich III-extractable Ca, Mg, Mn, and Zn. Yet, gorse accumulates higher concentrations of Ca, Zn and, Cu than the kikuyu grass (Pennesitum clandestinum), which is ubiquitous throughout the site. The Ca:Al and Mg:Al molar charge ratios of the soils are lowest within the epicenter of the gorse infestation, while the molar ratios are highest in the gorse apical stem tissues. All gorse plants are nodulated and have higher nitrogen contents than the surrounding kikuyu grass. Furthermore, the δ15N of the gorse stem tissues approaches 0‰, suggesting that nitrogen is being symbiotically fixed from the atmosphere. Characterization of the Bradyrhizobium isolated from gorse nodules shows similarities and distinctions to Bradyrhizobium isolated from the endemic legume koa (Acacia koa) within the same location. Population densities of the indigenous Bradyrhizobium are higher within the gorse rhizosphere than the kikuyu grass. Soil acidification, nutrient depletion, and symbiotic nitrogen fixation distinguish gorse-infested areas from the surrounding uninfested areas. These observations suggest that gorse has a competitive advantage over kikuyu grass under conditions of soil nutrient deficiency.  相似文献   

20.
Ten leguminous trees, four exotic species (Australian Acacia) and six indigenous species (three Sahelian Acacia spp. and three Sesbania spp.), were grown for 4 months in a natural Sahelian soil inoculated with or without the endomycorrhizal fungus, Glomus intraradices. In control trials, the determinant factor structuring the soil nematode fauna was the plant species, related plants having a similar influence on the nematode community in the soil. Soil nematode abundance increased from exotic acacias (3.3 g-1 dry soil) to native acacias (11.5 g-1 dry soil) and Sesbania species (17.6 g-1 dry soil). Plant feeding nematodes (mainly Scutellonema and Tylenchorhynchus) were significantly less abundant under exotic acacias (1.4 g-1 dry soil) than under native acacias (7.2 g-1 dry soil) or Sesbania species (7.3 g-1 dry soil). Bacterial feeding nematode density increased from exotic acacias (1.2 g-1 dry soil) to native acacias (3.0 g-1 dry soil) and Sesbania species (7.7 g-1 dry soil) as total densities. However, the differences in the structure of the nematode communities between plant groups were suppressed in the presence of the mycorrhizal fungus. In fact, no difference in nematode densities remained between plant groups when G. intraradices developed in several dominant taxa belonging to different trophic groups, particularly: Tylenchorhynchus, Apelenchina, Cephalobus and Dorylaimoidea. This study clearly indicated that inoculation with the arbuscular mycorrhizal fungus G. intraradices diminished the plant-specific effect on the structure of the soil nematode community.  相似文献   

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