Effects of increased nitrogen supply on the lignification of poplar wood

The short-term influence of adequate and high nitrogen fertilization on poplar lignification was investigated. The high nitrogen supply decreased lignin staining in the newly formed secondary xylem, indicating that lignin deposition was affected. Acetyl bromide determinations gave a 9-10% decrease in lignin content; however, Klason lignin content was unchanged. Thioacidolysis showed that elevated N supply affected lignin structure such that there was a reduced frequency of lignin units involved in beta-O-4 bonds, a reduced syringyl/guaiacyl ratio, an increased frequency of p-hydroxyphenyl lignin units, more guaiacyl units with free phenolic groups, and more p-hydroxybenzoic acid ester-linked to poplar lignins. These features suggest that lignins from poplars grown under high N bear structural similarities to lignins formed during early stages of wood development. The findings also indicate that a gravitational stimulus inducing the formation of tension wood and high N availability lead to similar and additive effects on lignin content and structure.     

Structural characterization of the lignin from the nodes and internodes of Arundo donax reed

Milled wood lignin (MWL) and dioxane lignin (DL) from different morphological regions (nodes and internodes) of Arundo donax reed were subjected to a comprehensive structural characterization by (13)C, (1)H NMR, FTIR, and UV spectroscopies and functional analysis. The permanganate and nitrobenzene oxidation methods were also applied to the in situ lignins. Both node and internode lignins are HGS-type lignins, with a significant amount of H units (including p-coumaric acid type structures). The S/G ratio (1.13-1.32), the weight-average molecular weight (20,400-24,500), the methoxyl group content (0.90-0.98), the phenolic hydroxyl group content (0.23-0.27), and the aliphatic hydroxyl group content (1.00-1.09) are not very different in the lignins from nodes and internodes. However, some structural differences between node and internode lignins were observed. The former has much more phenolic acids (p-coumaric and ferulic), 8.8% in node versus 1.2% in internode and less beta-O-4 (0. 32 and 0.49 per aromatic unit in node and internode, respectively). In situ node lignin is more condensed than internode lignin.     

Aroma biosynthesis in strawberry: s-adenosylmethionine:furaneol o-methyltransferase activity in ripening fruits

Among the most important volatile compounds in the aroma of strawberries are 2,5-dimethyl-4-hydroxy-3(2H)-furanone (Furaneol) and its methoxy derivative (methoxyfuraneol, mesifuran). Three strawberry varieties, Malach, Tamar, and Yael, were assessed for total volatiles, Furaneol, and methoxyfuraneol. The content of these compounds sharply increased during fruit ripening, with maximum values at the ripe stage. An enzymatic activity that transfers a methyl group from S-adenosylmethionine (SAM) to Furaneol sharply increases during ripening of strawberry fruits. The in vitro generated methoxyfuraneol was identified by radio-TLC and GC-MS. The partially purified enzyme had a native molecular mass of approximately 80 kDa, with optimum activity at pH 8.5 and 37 degrees C. A high apparent K(m) of 5 mM was calculated for Furaneol, whereas this enzyme preparation apparently accepted as substrates other o-dihydroxyphenol derivatives (such as catechol, caffeic acid, and protocatechuic aldehyde) with much higher affinities (K(m) approximately 105, 130, and 20 microM, respectively). A K(m) for SAM was found to be approximately 5 microM, regardless of the acceptor used. Substrates that contained a phenolic group with only one OH group, such as p-coumaric and trans-ferulic acid, as well as trans-anol and coniferyl alcohol, were apparently not accepted by this activity. It is suggested that Furaneol methylation is mediated by an O-methyltransferase activity and that this activity increases during fruit ripening.     

氮硫互作提高大蒜氮、硫含量及其关键同化酶活性

【目的】 从生理学角度研究氮、硫两种营养元素配施对大蒜氮硫关键同化酶的影响,揭示氮硫关键同化酶与植株氮、硫同化能力的关系,以期为大蒜合理施肥与提质增效提供理论参考。 【方法】 采用蛭石–珍珠岩盆栽方式,研究了不同浓度氮 (5、10、20 mmol/L)、硫 (2、4、8 mmol/L) 配施条件下,大蒜在幼苗期、花茎伸长期、鳞茎膨大初期和中期大蒜植株氮、硫含量,以及氮、硫关键同化酶活性的动态变化。 【结果】 大蒜植株氮含量总体呈上升趋势,在鳞茎膨大期达到最高水平,而硝酸还原酶 (NR)、谷氨酰胺合成酶 (GS) 活性变化呈先上升后下降趋势,在花茎伸长期至鳞茎膨大初期活性较高。硫含量总体呈先上升后平稳趋势,ATP-硫酸化酶 (ATPS) 活性在花茎伸长期达到最大值,而半胱氨酸合成酶 (OAS-TL) 活性则呈先下降后上升趋势,在花茎伸长期酶活性总体最低。鳞茎膨大期前,氮硫交互作用对氮、硫同化量有影响显著,而单因素影响不明显;鳞茎膨大期,单因素影响明显。硝酸还原酶、谷氨酰胺合成酶活性整体呈先升高后降低趋势。氮素对于 NR 活性影响显著,而对 GS 影响不显著;硫素仅在花茎伸长期和鳞茎膨大初期对NR活性有显著影响,而氮硫交互作用对 NR、GS 均有显著或极显著影响。氮素、硫素对 ATP-硫酸化酶、半胱氨酸合成酶活性无显著影响,而氮硫交互作用对其影响极显著。NR 活性在花茎伸长期、鳞茎膨大初期与植株氮呈显著正相关关系,ATPS 活性在花茎伸长期、鳞茎膨大初期与植株硫含量呈显著正相关关系,Pearson 系数分别为 0.690、0.847 和 0.662、0.816。鳞茎膨大初期和中期,GS 活性与氮含量呈显著负相关,相关系数分别为 –0.857、–0.693。OAS-TL 活性与硫含量整体呈负相关,而在鳞茎膨大初期为 0.646,呈显著正相关。 【结论】 大蒜生长过程中,氮、硫两元素间存在互作关系。NR、ATPS 等酶活性的提高增加了植株氮、硫同化能力,而 GS 则通过降低酶活性而促进氮的同化。在大蒜鳞茎膨大期前,氮、硫配施能够通过调控关键同化酶活性而影响氮、硫同化,进而影响植株生长;鳞茎膨大阶段,可以通过单一施肥达到调控大蒜植株氮或硫含量的目的。      

竹类植物中CYP85A1基因的克隆及表达分析

油菜素内酯(BRs)是一种促进植物茎秆伸长的甾类植物激素,CYP85A1基因参与了BRs合成中重要的催化反应步骤。为阐明CYP85A1基因在竹类植物节间伸长中的作用,本研究以20个竹类植物为试验材料,利用生物信息学分析了CYP85A1基因的特点,并利用实时荧光定量PCR技术分析了该基因在孝顺竹及其变种凤尾竹的不同组织和不同发育阶段的相对表达量。结果表明, 20个竹种中CYP85A1基因序列在DNA水平上的同源性为92.23%,cDNA序列的同源性为98.79%,氨基酸序列的同源性为97.86%;这些基因均含有9个外显子和8个内含子,编码465~480个氨基酸,蛋白分子量约为54 kDa,理论等电点均约为9.2。氨基酸序列中均含有细胞色素P450家族保守的血红素结合域、富含脯氨酸和氧的结构域及Glu-X-X-Arg结构域;CYP85A1基因孝顺竹和凤尾竹母竹的叶中表达量均最高,茎中次之,在根中的表达量最低。孝顺竹和凤尾竹幼竹中,该基因在顶部的表达量明显高于第2、第4、第5节间中的表达量;CYP85A1基因在快速伸长阶段的表达量明显高于生长缓慢时期,节间伸长速率越快该基因的表达量越高。综上,CYP85A1基因在竹类植物茎秆及节间伸长发育过程中具有促进作用。本研究结果为阐明CYP85A1在竹类植物中的生物学功能奠定了一定的理论基础。     

双低油菜(Canola)硫营养临界期与最大效率期的研究

利用温室砂培盆栽试验，分别在油菜生长的五个自然生育期严格控制营养液供硫浓度（高S0．75mmol／L或低S0．075mmol／L），研究各生育期供硫水平对油菜生长的影响。结果表明，发芽－莲座期（0～35天）低S处理容易短时间内表现缺硫症状，对子粒产量与含油量有明显的影响；开花期前后充足供硫可明显提高菜籽产量。后期增加供硫浓度不仅可明显增加菜籽的硫浓度、含油量，而且菜籽的硫甙含量也显著提高。比较各生育期供硫不足与菜籽产量的关系，以莲座期与开花期的影响最大。莲座期可作为油菜硫营养的临界期，开花期为最大效率期。硫的两个吸收高峰恰与油菜生长对硫的临界期与最大效率期相吻合。生长前期供硫充足主要促进植株的营养生长，茎枝重量较高但子粒产量很低，而后期供硫充足则显著促进植株的开花结果，增加角果数、角果重及子粒饱满度。前期供硫不足会使作物的花期明显延迟，中后期增加供硫浓度时还会造成植株的再次开花结果而使成熟期显著延迟。     

乙烯利和激动素对玉米茎秆抗倒伏和产量的影响

倒伏是影响玉米产量的主要原因之一,为了探明不同时期喷施不同生长调节剂对玉米抗倒伏性和产量的影响,选用玉米品种‘德美亚1号’和‘德美亚2号’为试验材料,在5、7、9展叶期分别喷施乙烯利(ETH)和激动素(KT),以喷施清水为对照,测定灌浆期基部茎秆形态、力学、化学组分及成熟后的倒伏率和产量。结果表明,与对照相比,‘德美亚1号’5展叶期喷施KT,节间直径显著增加21.11%,节间密度增加13.23%;与对照相比,‘德美亚2号’7展叶期喷施ETH,使基部节间总长度显著降低14.41%,节间直径显著增加10.70%,节间密度显著增加15.46%。‘德美亚1号’和‘德美亚2号’7展叶期喷施ETH,分别与对照相比,折断力显著增加26.04%和16.77%,穿刺强度显著增加22.77%和14.62%。‘德美亚2号’9展叶期喷施ETH,节间半纤维素、纤维素和木质素组成的化学组分总含量比对照显著增加25.49%。KT对两个品种茎秆力学影响表现不同,ETH对两个品种茎秆力学调控效果优于KT处理。两种调节剂处理均降低了两种玉米收获期的倒伏率。节间直径与折断力呈极显著正相关(r=0.905**),节间化学组分总含量和节间直径与倒伏率呈显著或极显著负相关。两个品种9展叶期喷施KT,产量比对照极显著增加22.24%和19.98%,喷施ETH对产量影响不显著。综上,KT于两个品种9展叶期喷施可显著增加产量,ETH于两个品种7展叶期喷施对抗倒伏性调控效果较好。     

铝胁迫对不同耐铝小麦品种根伸长生长影响的研究

为探讨铝胁迫抑制根生长的机理,以耐铝型小麦品种ET8和铝敏感型ES8为试验材料,研究了铝胁迫对小麦根相对伸长率,根尖细胞显微结构的影响以及细胞壁木质素含量及苯丙氨酸解氨酶(PAL)、肉桂醇脱氢酶(CAD)、过氧化物酶(POD)活性的变化。结果表明,ET8和ES8经50μmol/L铝胁迫6、122、4 h后,根相对伸长率随铝胁迫时间延长而变小。利用植物显微技术发现,ET8和ES8经50μmol/L胁迫24 h后,根尖伸长区皮层细胞变扁平,细胞间隙变小,细胞壁褶皱,并呈齿轮状交合;ES8细胞受伤害程度较ET8显著。经50μmol/L铝胁迫6、12、24 h后,ET8和ES8根尖细胞长度受铝胁迫的程度随时间延长而加强,根尖细胞相对长度与根相对伸长率呈显著正相关的关系(r=0.9911**)。50μmol/L铝胁迫24 h后,ET8和ES8根尖苯丙氨酸解氨酶(PAL)、肉桂醇脱氢酶(CAD)、过氧化物酶(POD)活性及细胞壁木质素合成显著增加。上述结果表明,铝胁迫通过增加苯丙氨酸解氨酶、肉桂醇脱氢酶、过氧化物酶活性,促进根尖细胞壁木质素合成,加快细胞的木质化,细胞壁延展性变小,从而抑制细胞的伸长,减小根的生长。由于铝胁迫下ES8中木质素合成显著高于ET8,且根尖细胞结构受破坏较ET8显著,造成铝胁迫下ES8根生长受抑制比ET8显著,是ET8较ES8耐铝胁迫的主要原因。     

抑制CCoAOMT表达的转基因多年生杨树检测与分析

本文以生长4年的转反义CCoAOMT基因的白杨杂种（欧洲山杨与银白杨,Populus tremula x Populus alba）及野生型对照为实验材料,测定Klason木质素含量并分析了木质素组分,结果表明转基因植株木质素含量经历4个生长季后,依然比对照降低13%,同时转基因植株木质素组分的G/S比比对照轻微降低。对茎杆切片显微观察表明转基因植株中部分导管向内凹陷,存在轻度变形。转基因植株与野生型对照在株高、胸径等外部生长形态上没有明显差别。     

抑制咖啡酰-辅酶A甲基转移酶(CCoAOMT)表达的转基因多年生杨树检测与分析

本文以生长4年的转反义CCoAOMT基因的白杨杂种（欧洲山杨与银白杨,Populus tremula x Populus alba）及野生型对照为实验材料,测定Klason木质素含量并分析了木质素组分,结果表明转基因植株木质素含量经历4个生长季后,依然比对照降低13%,同时转基因植株木质素组分的G/S比比对照轻微降低。对茎杆切片显微观察表明转基因植株中部分导管向内凹陷,存在轻度变形。转基因植株与野生型对照在株高、胸径等外部生长形态上没有明显差别。     

Influences of seed molybdenum and molybdenum application on nitrate reductase activity,shoot dry matter,and grain yields of winter wheat cultivars

The influences of seed molybdenum (Mo) and application of Mo on nitrate reductase activity(NRA) in seedling stage, shoot dry matter in stem elongation stage, and grain yields in harvesting stage were compared among 35 winter wheat cultivars grown on acid yellow brown earths. Seedling NRA was significantly and positively correlated with seed Mo content regardless of further molybdenum application. The correlation of both shoot dry matter in stem elongation stage and grain yields to seed Mo content were also significant in a much less degree under Mo deficiency, but not after molybdenum application. Molybdenum application significantly increased seedling NRA, shoot dry matter in stem elongation stage and grain yields for most cultivars. There were no obvious relationships between molybdenum efficiency ratios and seed molybdenum content. These showed that seed Mo was very important for plant growth, but it was not sufficient to complete the whole life of plant. Therefore, Mo must be applied in order to get satisfactory grain yields from most cultivars. However, a high Mo efficiency genotype (97003) was promising to adapt to Mo deficient soils without added Mo.     

Anatomical, histological, and histochemical changes in grape seeds from Vitis vinifera L. cv Cabernet franc during fruit development

Cabernet franc berries were sampled at five stages from berry set to harvest from an experimental vineyard in mid Loire Valley. Seeds were collected from representative berries in term of stage of development. The evolution of seed was followed both macro- and microscopically. For microscopy analysis, seeds were cut, put in a fixation solution, and cut into thin sections with a microtome. Five staining solutions were used for each seed sample: toluidine blue O, phloroglucinol, periodic acid-Schiff's reagent and naphtol blue black, vanillin, and p-dimethylaminocinnamaldehyde. Toluidine blue O staining revealed the evolution of tissue structures during grape seed development. We studied the changes in chemical compounds (lignin, polysaccharides, proteins, and tannins) with the other reagents. Seed lignification was achieved at veraison. Proanthocyanidins were localized in epidermis, inner cells of the soft seed coat, and inner cell layer of the inner integument. Finally, the localization of flavan-3-ols was linked with changes in cell walls of the outer integument.     

Der Nitratgehalt in der Halmbasis als Maßstab für den Stickstoffdüngerbedarf bei Wintergetreide

The nitrate content of the basal internode of the stems as an indicator of the nitrogen fertilizer requirement of winter cereals In fertilizer experiments on loess soils in the southern part of Lower Saxony (FRG) the possibility was tested if the nitrate content of the basal internode of the stems can be used in the determination of the N-fertilizer requirement between stem elongation and ear emergence. The nitrate concentration was determined by a plant sap test. The following results were obtained:

• 1 Nitrate concentrations in the fresh matter between 200 and 2000 ppm can be determined by the reagent ?Diphenylaminesulphuric acid”?. This method is easily applicable and can be done by farmers.
• 1 Winter wheat and winter barley have different nitrate concentrations in the basal internode of the stems between stem elongation and ear emergence. As the N-supply in spring (N_min + N-fertilizer) was the same in all fields, the different nitrate concentrations in the stems are due to the preceding crop and to different organic manuring.
• 1 There is a close relationship between the nitrate content in stems (basal internode) and the nitrate content of the soil.
• 1 The required amount of nitrogen fertilizer between stem elongation and ear emergence was significantly correlated with the nitrate content in the stems (basal intenode). Therefore, a recommendation of N-top dressings to winter wheat and winter barley was made on this basis. The method is suitable for determining the necessary amount and timing of N-fertilization.

     

茭白肉质茎膨大发育的生化基础研究

文研究了茭白肉质茎不同发育阶段的碳水化合物、蛋白质以及几种酶的变化情况 ,分析了这些生化代谢与茭白肉质茎发育的可能关系。结果表明 :茭白肉质茎发育的早期主要表现为重量的快速增加 ,后期则为重量和体积均快速地增长。碳水化合物中总糖和还原糖在肉质茎发育的早期迅速上升 ,之后有所下降。而蔗糖则呈现先上升 ,后稳定的趋势。淀粉的含量在肉质茎发育过程中一直上升。木质素和纤维素在肉质茎发育的早期含量较高 ,随着茎的发育 ,其含量持续下降 ,发育后期二者的含量又明显上升。蛋白含量前期上升 ,后期下降。对茭白肉质茎发育过程中酶活性的研究表明 ,超氧化物歧化酶和过氧化氢酶活性不断下降 ,而过氧化物酶活性持续上升。苯丙氨酸解氨酶活性在茎发育过程中不断下降 ,后期又有所上升。     

Evaluation of soyasaponin, isoflavone, protein, lipid, and free sugar accumulation in developing soybean seeds

A combination of analytical techniques was used to examine and quantify seed compositional components such as protein, lipid, free sugars, isoflavones, and soyasaponins during soybean development and maturation in two Korean soybean cultivars. Protein accumulation was rapid during reproductive stages, while lipid content was only relatively moderately increased. The major carbohydrate saccarides sucrose and stachyose constantly increased during the reproductive stage. Previously published results suggest that the free sugar and lipid content reached their maximal concentrations at a relatively early stage of seed development and remain constant in comparison to other chemical components. The malonylglucosides were the predominant isoflavone form followed by the glucosides, acetyl glucosides, and aglycone forms. As soybean seed matures, total soyasaponin concentration was constantly decreased until the R8 stage. Soyasaponin beta(g) was the major soyasaponin in DDMP-conjugated group B soyasaponins, followed by the non-DDMP counterpart soyasaponin I and soyasaponin A1. The ratio of total isoflavone to total soyasaponin in the developing soybean increased from 0.06 to 1.31. Protein, lipid, and free sugar contents in the developing soybean seeds showed significant positive correlations with conjugated isoflavones and total isoflavone concentration, while the lipid contents showed a negative correlation with the isoflavone aglycone. Protein, lipid, and free sugar contents showed a negative correlation with total group A and B soyasaponins and total soyasaponins; however, only the soyasaponin A content was significantly negatively correlated with free sugar content. Total soyasaponin content was negatively correlated with isoflavone content (r = -0.828 at p < 0.01).     

Fruit development in Capsicum annuum: changes in capsaicin, lignin, free phenolics, and peroxidase patterns

Pepper fruits, of Capsicum annuum cv. Padron, undergo changes in content of capsaicinoids, lignin, and free phenolics during the maturation process. Although capsaicinoids increase with development, the maximal levels of free phenolics and lignin are observed during the early stages of development. A decrease of peroxidase activity was observed during maturation, and this was related with a decrease in other physiological parameters studied, namely chlorophylls and pH. Subcellular fractionation studies reveal that most peroxidase activity is localized in the soluble fraction throughout development. The changes in the peroxidase activity were accompanied by changes in the different isoenzymes. Acidic isoenzymes increased whereas the basic isoenzymes decreased over the same period, and the changes in these isoenzymes were related with capsaicin metabolism.     

切花菊茎秆性状的生长动态与遗传分析

为明确切花菊茎秆性状在不同遗传背景下的生长动态和遗传特性,本研究以亲本茎秆性状差异较大的寒小白×蒙娜丽莎白(MH)和亲本茎秆性状差异较小的QX097×蒙娜丽莎白(MQ)两个杂交F₁群体为试验材料,调查了定植后15、30、45、60、70 d时的株高、茎粗和节间数,比较分析切花菊茎秆性状的生长动态、杂种优势和主基因效应。结果表明,两个组合的茎秆性状生长动态基本一致,与亲本性状的差异程度关系不大。其中,株高和茎粗总体符合S型生长曲线,而节间数的生长曲线不明显;各茎秆性状的相对生长速率在定植后45 d前均较快。MQ组合株高、茎粗和节间数等茎秆性状的平均值在大部分测定时期均高于MH组合;而MH组合株高和定植45 d后节间数的变异系数都高于MQ组合,但是在茎粗性状上没有明显规律性。各茎秆性状的中亲优势率在MH组合中多表现为正值,而在MQ组合中,除了定植后15 d时茎粗的中亲优势率为正值,其他各时期茎秆性状的中亲优势率均表现为负值。主基因+多基因混合遗传模型分析在MH组合的株高和MQ组合的茎粗上检测到2对加性主基因效应,主基因遗传力分别为97.12%和9.33%,而在其他组合或茎秆性状上未检测到主基因效应。本研究结果为菊花营养生长期栽培调控和茎秆性状的遗传改良提供了参考依据。     

玉米-大豆带状间作对大豆生长、光合荧光特性及产量的影响

为了探究玉米-大豆间作对大豆生长策略、光合荧光特性及产量的影响,以大豆品种中黄39为试材,采用玉米-大豆带状间作和大豆净作2种不同的种植模式,研究间作下大豆的形态、物质分配及光合荧光参数的变化规律。结果表明,间作大豆植株的株高在四节期(V4期)、始荚期(R3期)和鼓粒期(R6期)分别比净作增加22.47%、47.33%和32.72%。在V4期,间作大豆除株高显著高于净作外,大豆茎粗、主茎节数、茎叶柄生物量和叶绿素含量在净作和间作下均差异不显著。在R3和R6期,间作大豆植株的茎、柄生物量显著增加,但叶生物量、叶绿素a含量、叶绿素总含量及净光合速率(Pn)显著低于净作大豆。对于叶绿素荧光参数,间作大豆叶片非光化学淬灭系数(NPQ)在V4、R3和R6期分别显著高出净作大豆12.2%、5.04%和7.2%,而间作大豆叶片的PSII实际的光化学量子效率(F'q/F'm)、光化学淬灭系数(q P)和PSII反应中心潜在的激发能捕获效率(F_v/F_m)与净作大豆相比差异不显著。在产量构成因素中,间作大豆的单株荚数、单株粒数、百粒重及单株产量均显著低于净作,分别降低27.78%、12.33%、20.72%。间作下玉米对大豆生育后期的生长、光合特性的影响直接导致大豆产量及构成因素的下降。因此,在玉米-大豆带状间作种植模式下,要提高间作大豆产量,需降低大豆生育后期玉米荫蔽程度。本研究结果为间作大豆栽培及高产提供了一定的依据。     

钟模型法建立甘蔗发育期模拟模型

基于作物发育动态理论模型原理及钟模型方法构建甘蔗发育期模拟模型（SDSM,sugarcane development simulation model）,模拟新植蔗和多年宿根蔗不同发育期。利用广西甘蔗主产区（宜州、沙塘、来宾、扶绥、贵港）的甘蔗发育期多年观测资料及同期气象数据,结合甘蔗各发育阶段的三基点温度指标,通过试错法确定甘蔗发育期模拟模型（SDSM）参数,模拟新植蔗、宿根蔗各发育期（播种-出苗、出苗-分蘖、分蘖-茎伸长、茎伸长-工艺成熟)。通过模拟值与实测值对比分析,对模拟效果进行评价。结果表明：模型具有较强的机理性,模型中基本发育函数部分反映了品种的基因特性,模型能够有效模拟甘蔗的发育期。新植蔗各发育阶段NRMSE在5.2%～26.31%,播种-出苗阶段模拟值与实测值相差8.1d,出苗-分蘖相差7.4d,分蘖-茎伸长相差4.6d,茎伸长-工艺成熟相差7.4d;宿根蔗各发育阶段NRMSE在6.52%～21.66%,上一年工艺成熟-发株阶段模拟值与实测值相差8.8d,发株-分蘖相差8.7d,分蘖-茎伸长相差7.5d,茎伸长-工艺成熟相差9.9d。说明模拟值与实测值具有较好的一致性与相关性,模型可以实现对甘蔗发育期的预测。