Yield,water use and radiation use efficiencies of kenaf (Hibiscus cannabinus L.) under reduced water and nitrogen soil availability in a semi-arid Mediterranean area

Kenaf is a warm-season species that recently has been proved to be a good source of biomass for cellulose pulp for the paper industry in Mediterranean countries, where the use of hemp is problematic for legal reasons. A two-year research program aiming at studying the effects of different water regimes and nitrogen fertilization levels, upon plant growth, leaf area index, biomass accumulation, water and radiation use efficiency, was carried out on kenaf under a typically semi-arid Mediterranean climate of South Italy. In cv. Tainung 2, four different water regimes (I₀ = no irrigation, I₂₅, I₅₀ and I₁₀₀ = 25, 50 and 100% ETc restoration, respectively) and three nitrogen levels (N₀ = no nitrogen, N₇₅ and N₁₅₀ = 75 and 150 kg ha⁻¹ of N, respectively) were studied. The amount of water applied strongly affected plant growth (in terms of LAI, plant height and biomass) and final total and stem dry yield, which significantly increased from I₀ to I₁₀₀. Nitrogen did not exert any beneficial effect upon dry yield. Radiation Use Efficiency (RUE), calculated in the second year only, was the highest (1.95 g DM MJ⁻¹) in fully irrigated treatment (I₁₀₀) and the lowest (0.86 g DM MJ⁻¹) in the dry control.Water use efficiency (WUE) was rather similar among water regimes, whilst irrigation water use efficiency (IWUE) progressively increased with the decrease of total volume of water distributed to the crop by irrigation, from 3.47 to 12.45 kg m⁻³ in 2004 and from 4.27 to 7.72 kg m⁻³ in 2005. The results obtained from this research demonstrate that in semi-arid areas of South Italy, irrigation at a reduced rate (50% ETc restoration) may be advantageous, since it allowed a 42–45% irrigation water saving, when compared to the fully irrigation treatment, against a 23% (in 2004) and 36% (in 2005) yield reduction, and a still good efficiency (near that potential) in transforming the solar radiation in dry biomass was maintained (RUE = 1.76 g DM MJ⁻¹, against 1.95 g DM MJ⁻¹ in fully irrigated treatment).     

Maize yield and water balance is affected by nitrogen application in a film-mulching ridge–furrow system in a semiarid region of China

Poor soil and drought stress are common in semiarid areas of China, but maize has a high demand for nitrogen (N) and water. Maize production using the technique of double ridges and furrows mulched with plastic film are being rapidly adopted due to significant increases in yield and water use efficiency (WUE) in these areas. This paper studied N use and water balance of maize crops under double ridges and furrows mulched with plastic-film systems in a semiarid environment over four growing seasons from 2007 to 2010. To improve precipitation storage in the non-growing season, the whole-year plastic-film mulching technique was used. There were six treatments which had 0, 70, 140, 280, 420 or 560 kg N ha⁻¹ applied in every year for maize. In April 2011, spring wheat was planted in flat plots without fertilizer or mulch following four years of maize cultivation. After four years, all treatments not only maintained soil water balance in the 0–200 cm soil layer but soil water content also increased in the 0–160 cm soil layer compared to values before maize sowing in April 2007. However, under similar precipitation and only one season of spring wheat, soil water content in the 0–160 cm soil layer sharply decreased in all treatments compared to values before sowing in April 2011. Over the four years of maize cultivation, average yield in all treatments ranged from 4071 to 6676 kg ha⁻¹ and WUE ranged from 18.2 to 28.2 kg ha⁻¹ mm⁻¹. In 2011, the yield of spring wheat in all treatments ranged from 763 to 1260 kg ha⁻¹ and WUE from 3.5 to 6.5 kg ha⁻¹ mm⁻¹. The potential maximum grain yield for maize was 6784 kg ha⁻¹ with 360 kg N ha⁻¹ applied for four years, but considerable NO₃N accumulated in the soil profile. A lesser application (110 kg N ha⁻¹) to this tillage system yielded in 82% of the maximum, increased nitrogen use efficiency and mitigated the risk of nitrogen loss from the system. This study suggests that double ridge–furrow and whole-year plastic-film mulching could sustain high grain yields in maize with approximately 110 kg N ha⁻¹ and maintain soil water balance when annual precipitation is >273 mm in this semiarid environment.     

Optimized single irrigation can achieve high corn yield and water use efficiency in the Corn Belt of Northeast China

Decreasing the corn (Zea mays L.) gap between the potential yield and farm yield and reducing the risk of grain yield of drought are very important for corn production in the Corn Belt of Northeast China (CBNC). To achieve a high and stable corn yield, the effects of supplementary irrigation on yield, water use efficiency (WUE) and irrigation water use efficiency (IWUE) were studied using a modelling approach. The Root Zone Water Quality Model 2 was parameterized and evaluated using two years of experimental data in aeolian sandy soil and black soil. The evaluated model was then used to investigate responses to various irrigation strategies (rainfed, full irrigation and 12 single irrigation scenarios) using long-term weather data from 1980 to 2012. Full irrigation guarantees a high and stable corn grain yield (12.92 Mg ha⁻¹ and has a coefficient of variation (CV) of 14.8% in aeolian sandy soil; 12.30 kg Ma⁻¹ and CV of 11.1% in black soil), but has a low water use efficiency (19.92 and 21.81 kg ha⁻¹ mm⁻¹) and a low irrigation water use efficiency (10.01 and 11.03 kg ha⁻¹ mm⁻¹). A single irrigation can increase corn yields by 3–35% for aeolian sandy soil and 5–35% for black soil over different irrigation dates compared with no irrigation. The most suitable single irrigation date was during late June to early July for aeolian sandy soil (yield = 10.73 Mg ha⁻¹ and WUE = 27.94 kg ha⁻¹ mm⁻¹) and early to mid-July for black soil (yield = 11.20 Mg ha⁻¹ and WUE = 27.70 kg ha⁻¹ mm⁻¹). The lowest yield risk of falling short of the yield goal of 8, 9, and 10 Mg ha⁻¹ were 9.1%, 18.2%, and 33.33% in aeolian sandy soil and 3.0%, 15.25, and 21.2% in black soil when an optimized single irrigation was applied in late June or early July, respectively. Therefore, an optimized single irrigation should be applied in late June to early July with the irrigation amount to refill soil water storage of root zone to field capacity in CBNC.     

Pig slurry fertilization in a double-annual cropping forage system under sub-humid Mediterranean conditions

In areas of Southern Europe with very intensive pig production, most of the pig slurry (PS) is applied as fertilizer. However, in the European Union, no more than 170 kg N ha⁻¹ year⁻¹ can be applied in nitrate vulnerable zones (NVZs) from livestock manures. In this context, a six-year trial was conducted for a maize-triticale double-annual forage cropping rotation under rainfed conditions. Four different N rates were applied (0, 170, 250 and 330 kg N ha⁻¹ year⁻¹), to evaluate their effect on crop yield, N uptake, unrecovered N and soil nitrate content. The corresponding PS rates were defined as zero (PS 0), low (PSL) medium (PSM) and high (PSH). The annual average dry matter (DM) yields (maize + triticale) for the PS fertilization treatments PS0, PSL, PSM and PSH were 12.6, 17.7, 20.2 and 22.0 Mg DM ha⁻¹, respectively. Maize DM yield was influenced mainly by weather conditions, and triticale DM yield was clearly influenced by initial soil NO₃⁻-N and PS fertilization rates. Unrecovered N was affected by PS fertilization rate and initial soil NO₃⁻-N content. A residual effect of the PS when applied to maize had an important effect on soil NO₃⁻-N and subsequent triticale DM yield. Moreover, total annual average unrecovered N, considering the sum of both crops (maize + triticale), were 91, 144, and 222 kg N ha⁻¹ in PSL, PSM and PSH, respectively. In order to avoid part of this unrecovered N, mainly by lixiviation of nitrates, PS fertilization in triticale should be applied as side dressing at tillering. The application of N, in the form of PS, at rates higher than the legally permitted maximum of 170 kg N ha⁻¹ year⁻¹, may result in better yields. However, high rates of PS fertilization may originate in significantly lower N use efficiency and a higher potential environmental impact in double-cropping systems, practiced in rainfed sub-humid Mediterranean conditions.     

Yield and nitrogen utilization efficiency of the pseudocereals amaranth,quinoa, and buckwheat under differing nitrogen fertilization

In recent years, the cultivation of the pseudocereal species amaranth, quinoa, and buckwheat has gained rising attention. This study was undertaken to explore nitrogen (N) fertility requirements and nitrogen use efficiency of these species. For this purpose, a 2-year field experiment with N rates of 0, 80, and 120 kg N ha⁻¹ for amaranth and quinoa and 0, 30, and 60 kg N ha⁻¹ for buckwheat and two cultivars of each species was conducted.Grain yield of amaranth responded to N and ranged between 1986 and 2767 kg ha⁻¹. Nitrogen utilization efficiency (NUtE) ranged from 13.9 to 15.4 kg grain yield per kg above-ground plant N and decreased with increasing N rate. Higher grain yields and NUtEs seemed to be mainly inhibited by the low harvest index (0.22–0.23) of the investigated amaranth cultivars.Quinoa yielded between 1790 and 3495 kg grain ha⁻¹ and responded strongly to N fertilization. NUtE averaged 22.2 kg kg⁻¹ and did not decrease with increasing N rates.The grain yield of buckwheat did not respond to N fertilization and averaged 1425 kg ha⁻¹. N uptake increased only slightly with N fertilization. NUtE ranged from 16.1 to 20.0 kg kg⁻¹. Main problems occurring with the application of N to buckwheat were grain scattering and lodging.     

Old tall durum wheat cultivars are suited for dual-purpose utilization

The lateness, tallness and high vigour of old tall durum wheat cultivars could be advantageous for dual-purpose use and their high propensity for lodging should be reduced by grazing. A 3-year field trial was performed in Sardinia, Italy, in a typical Mediterranean environment. Crops of the durum wheat cultivar Senatore Cappelli were sown in October, and grazing was simulated by clipping half of the plots at the terminal spikelet stage of development. The forage biomass derived from clipping varied greatly between seasons (from 0.8 to 3.3 t ha⁻¹ dry matter) in response to the notable inter-seasonal variability in weather conditions. Cultivar Senatore Cappelli showed good recovery following clipping, with the ability to attain almost complete radiation interception well before anthesis. The high number of leaves that emerged after clipping might have contributed to this good recovery. Nevertheless, clipping reduced the dry matter produced by anthesis (16 t ha⁻¹ in clipped compared to 21 t ha⁻¹ in unclipped crops) as well as the final dry matter (DM_MAT) (19 t ha⁻¹ in clipped compared to 23 t ha⁻¹ in unclipped crops), although these differences disappeared when the clipped biomass was included. The lower lodging observed at anthesis in the clipped (21%) compared with unclipped crops (63%) likely reduced the difference between treatments. The lower DM_MAT of clipped treatments was reflected in a lower grain yield (GY) (3.4 t ha⁻¹ vs 4.2 t ha⁻¹ in the unclipped treatment). Clipping did not affect the amount of nitrogen present in the biomass, nitrogen uptake efficiency or radiation use efficiency. GY reduction after clipping was mediated by the reduction in spikes m⁻² and kernels m⁻² (KNO). Spike fertility was not affected by clipping, because the same amount of radiation was available for each spike (about 1 MJ). The period with reduced ground cover after clipping was reflected in an increased evaporation and reduced transpiration, which did not alter the total water used and increased the transpiration efficiency in terms of DM_MAT.Old tall durum wheat cultivars manifested good suitability for dual-purpose use in environments with low attainable yields because their low grain yield potential contributed to reducing the negative effects of clipping on GY. Their high straw yield and kernel protein percentage represented an advantage with respect to semi-dwarf cultivars.     

Effect of irrigation and nitrogen fertilization on the production of biogas from maize and sorghum in a water limited environment

The expansion of biogas production from anaerobic digestion in the Po Valley (Northern Italy) has stimulated the cultivation of dedicated biomass crops, and maize in particular. A mid-term experiment was carried out from 2006 to 2010 on a silt loamy soil in Northern Italy to compare water use and energy efficiency of maize and sorghum cultivation under rain fed and well-watered treatments and at two rates of nitrogen fertilization. The present work hypothesis were: (i) biomass sorghum, for its efficient use of water and nitrogen, could be a valuable alternative to maize for biogas production; (ii) reduction of irrigation level and (iii) application of low nitrogen fertilizer rate increase the efficiency of bioenergy production. Water treatments, a rain fed control (I0) and two irrigation levels (I1 and I2; only one in 2006 and 2009), were compared in a split–split plot design with four replicates. Two fertilizer rates were also tested: low (N1, 60 kg ha⁻¹ of nitrogen; 0 kg ha⁻¹ of nitrogen in 2010) and high (N2, 120 kg ha⁻¹ of nitrogen; 100 kg ha⁻¹ of nitrogen in 2010). Across treatments, sorghum produced more aboveground biomass than maize, respectively 21.6 Mg ha⁻¹ and 16.8 Mg ha⁻¹ (p < 0.01). In both species, biomass yield was lower in I0 than in I1 and I2 (p < 0.01), while I1 and I2 did differ significantly. Nitrogen level never affected biomass yield. Water use efficiency was generally higher in sorghum (52 kg ha⁻¹ mm⁻¹) than in maize (38 kg ha⁻¹ mm⁻¹); the significant interaction between crop and irrigation revealed that water use efficiency did not differ across water levels in sorghum, whereas it significantly increased from I0 and I1 to I2 in maize (p < 0.01). The potential methane production was similar in maize and sorghum, while it was significantly lower in I0 (16505 MJ ha⁻¹) than in I1 and I2 (21700 MJ ha⁻¹). The only significant effect of nitrogen fertilization was found in the calculation of energy efficiency (ratio of energy output and input) that was higher in N1 than in N2 (p < 0.01). These results support the hypothesis that (i) sorghum should be cultivated rather than maize to increase energy efficiency, (ii) irrigation level should replace up to 36% of ETr and (iii) nitrogen fertilizer rate should be minimized to maximize the efficiency in biomass production for anaerobic digestion in the Po Valley.     

Alternative strategies for nitrogen fertilization of overwinter processing spinach (Spinacia oleracea L.) in Southern Italy

To identify the best practice for nitrogen (N) fertilization of overwinter processing spinach, two field experiments were carried out in the Foggia plain (Southern Italy), one of the most vocated area for leafy vegetables production. The field trials were aimed to define and suggest the proper fertilizer dose, typology and the right time of application. Experiment 1 evaluated four N fertilizer doses (0, 150, 225, 300 kg ha⁻¹) in a two-year field trial. Experiment 2 was aimed to assess the effect of the split distribution of prilled urea fertilizer in comparison with the application of nitrification inhibitor (DMPP) containing urea fertilizer, broadcasted at sowing.Spinach yield, yield quality (nitrate – NO₃⁻ – and carotenoids content), N-use efficiency and risk of soil nitrate (NO₃⁻-N) leaching were evaluated. The processing spinach yielded 37.8 and 3.6 t ha⁻¹ of fresh and dry yield, respectively (average of the two experiments). Fresh and dry yield among the fertilizing treatments were similar. Also the β-carotene and the lutein content of spinach leaves (19.5 and 38.1 mg kg⁻¹, respectively) were not affected by the N fertilizer dose. Conversely, the N dose strongly influenced the NO₃⁻ content of the leafy vegetable tissues (1286 mg kg⁻¹ on average, 58% lower than the limits imposed by the EC regulation). As expected, the different rainfall pattern influenced both the leaf NO₃⁻ content and the risk of soil NO₃⁻-N leaching. The results achieved demonstrated that, in order to get a favorable trade-off, among yield, yield quality, N-use efficiency and environmental impact, the processing spinach growers of the Foggia plain area should be encouraged to apply 225 kg N ha⁻¹ as maximum fertilization rate. Also, the split urea fertilizer application appeared as the more effective strategy for N fertilization of overwinter spinach in comparison with the use of the nitrification inhibitor containing urea fertilizer, being the last strategy not able to adequately match the N crop demand.     

White lupine as a beneficial crop in Southern Europe. II. Nitrogen recovery in a legume–oat rotation and a continuous oat–oat

One experiment lasting for two years was carried out at Pegões (central Portugal) to estimate the impact of mature white lupine residue (Lupinus albus L.) on yield of fodder oat (Avena sativa L. cv. Sta. Eulalia) as the next crop in rotation, comparing with the continuous cultivation of cereal, under two tillage practices (conventional tillage and no-till) and fertilized with five mineral nitrogen (N) rates, with three replicates. Oat as a first crop in the rotation provided more N to the agro-ecosystem (63 kg N ha⁻¹) than did lupine (30–59 kg N ha⁻¹). This was at a cost of 100 kg of mineral N ha⁻¹, whereas lupine was grown without addition of N. A positive response of oat as a second crop was obtained per kg of lupine-N added to the system when compared with the continuous oat–oat. The cereal also responded positively to mineral N in the legume amended soil in contrast with the oat–oat sequence where no response was observed, partly due to the fast mineralization rate of lupine residue and a greater soil N immobilization in the continuous oat system. Each kg N ha⁻¹ added to the soil through the application of 73 kg DM ha⁻¹ mature lupine residue (above- and belowground material) increased by 72 kg DM ha⁻¹ the oat biomass produced as the second crop in rotation when 150 kg mineral N ha⁻¹ were split in the season, independent of tillage practice. Mature legume residue conserved in the no-tilled soil depressed the yield of succeeding cereal but less than the continuous oat–oat for both tillage practices, where the application of mineral N did not improve the crop response.     

The effect of succeeding crop and level of N fertilization on N leaching after break-up of grassland

Depending on soil and management, ploughing up grassland for use as arable land can lead to an increase in the release of mineralized nitrogen and a high risk of nitrogen leaching during winter. The amount of N leaching is also dependent on the N efficiency of following crops and the level of N fertilization.In a field experiment in northwest Germany permanent grassland was ploughed and used as arable land. The experiment was conducted over 2 years at three sites and investigated two main factors: (i) succeeding crops, either spring barley (and catch crop)–maize or silage maize–maize; and (ii) N-fertilization either nil or moderate (120 kg N ha⁻¹ for barley or 160 kg for maize). Plant yields, the soil mineral nitrogen (SMN) content and the nitrate leaching losses over winter were determined. On average for the 2-year period, the SMN in autumn and the nitrate leaching losses during winter for the rotation barley–maize were 76 kg ha⁻¹ SMN and 81 kg N ha⁻¹ N leaching losses, and for maize–maize they amounted to 108 and 113 kg ha⁻¹, respectively. The SMN and N leaching losses for the plots with no N fertilizer were 49 and 52 kg N ha⁻¹ and for the plots fertilized at a moderate N level they were 135 and 142 kg N ha⁻¹, respectively.We conclude that although the extent of nitrate leaching is influenced by the site conditions and management of the grassland prior to ploughing, the management after ploughing is the decisive factor. The farmer can significantly reduce nitrate leaching with his choice of succeeding crop and the amount of N fertilization.     

Organic amendment and minimum tillage in winter wheat grown in Mediterranean conditions: Effects on yield performance,soil fertility and environmental impact

The experiment was conducted to evaluate the agronomic benefit of the application of organic fertilizers combined with different soil tillage on quantitative and qualitative components of winter wheat (Triticum durum Desf., cv. ‘Simeto’) and on chemical soil fertility parameters. The environmental impact, due to heavy metals introduced in soil-plant system, was further investigated. Soil tillage treatments consisted of conventional (CT) and minimum tillage (MT). Fertilization treatments were: mineral at 100 kg N ha⁻¹ (N_min); municipal solid waste compost at 100 kg N ha⁻¹ (N_comp); 50 kg N ha⁻¹ of both compost and mineral fertilizers (N_mix); sewage sludge at 100 kg N ha⁻¹ (N_ss). These treatments were compared with an unfertilized control (N₀). No significant difference was observed between the two soil tillage treatments for quantitative yield production, while among the fertilization treatments N_ss did not show any significant difference compared to N_min. At the end of the research, the fertility of the soil (oxidable carbon, total nitrogen, available phosphorus) was on average higher in N_comp and N_ss treatments compared to the N₀ and N_min ones. The overall distribution of heavy metals in soil-plant system respect to the different fertilizer treatments has not allowed to grouped their effects with Principal Components Analysis. This result showed that the amount of potential pollutants applied by organic amendments did not modified the dynamic equilibrium of the soil–plant system. The MT, as well as the fertilization with the application of sewage sludge (N_ss), allowed to reach productive performance similar to conventional management (CT with N_min). Here we demonstrate that, in the short term period, sustainable agronomical techniques can replace the conventional one with environmental benefit.     

Tillage,cover crops,and nitrogen fertilization effects on soil nitrogen and cotton and sorghum yields

Sustainable soil and crop management practices that reduce soil erosion and nitrogen (N) leaching, conserve soil organic matter, and optimize cotton and sorghum yields still remain a challenge. We examined the influence of three tillage practices (no-till, strip till and chisel till), four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secaele cereale L.)], vetch/rye biculture and winter weeds or no cover crop}, and three N fertilization rates (0, 60–65 and 120–130 kg N ha⁻¹) on soil inorganic N content at the 0–30 cm depth and yields and N uptake of cotton (Gossypium hirsutum L.) and sorghum [Sorghum bicolor (L.) Moench]. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic, Plinthic Paleudults) from 1999 to 2002 in Georgia, USA. Nitrogen supplied by cover crops was greater with vetch and vetch/rye biculture than with rye and weeds. Soil inorganic N at the 0–10 and 10–30 cm depths increased with increasing N rate and were greater with vetch than with rye and weeds in April 2000 and 2002. Inorganic N at 0–10 cm was also greater with vetch than with rye in no-till, greater with vetch/rye than with rye and weeds in strip till, and greater with vetch than with rye and weeds in chisel till. In 2000, cotton lint yield and N uptake were greater in no-till with rye or 60 kg N ha⁻¹ than in other treatments, but biomass (stems + leaves) yield and N uptake were greater with vetch and vetch/rye than with rye or weeds, and greater with 60 and 120 than with 0 kg N ha⁻¹. In 2001, sorghum grain yield, biomass yield, and N uptake were greater in strip till and chisel till than in no-till, and greater in vetch and vetch/rye with or without N than in rye and weeds with 0 or 65 kg N ha⁻¹. In 2002, cotton lint yield and N uptake were greater in chisel till, rye and weeds with 0 or 60 kg N ha⁻¹ than in other treatments, but biomass N uptake was greater in vetch/rye with 60 kg N ha⁻¹ than in rye and weeds with 0 or 60 kg N ha⁻¹. Increased N supplied by hairy vetch or 120–130 kg N ha⁻¹ increased soil N availability, sorghum grain yield, cotton and sorghum biomass yields, and N uptake but decreased cotton lint yield and lint N uptake compared with rye, weeds or 0 kg N ha⁻¹. Cotton and sorghum yields and N uptake can be optimized and potentials for soil erosion and N leaching can be reduced by using conservation tillage, such as no-till or strip till, with vetch/rye biculture cover crop and 60–65 kg N ha⁻¹. The results can be applied in regions where cover crops can be grown in the winter to reduce soil erosion and N leaching and where tillage intensity and N fertilization rates can be minimized to reduce the costs of energy requirement for tillage and N fertilization while optimizing crop production.     

Development of critical nitrogen dilution curve in rice based on leaf dry matter

The critical nitrogen (N_c), defined as the minimum N concentration required for maximum growth, is proposed for diagnosis of the in-season N status in crop plants. It has been established for several crops including rice on whole-plant dry matter (DM) basis but has not been determined for canopy leaf basis. This research was undertaken to develop a new N_c dilution curve based on leaf dry matter (L_DM) and to assess its applicability to estimate the level of N nutrition for Japonica rice in east China. Three field experiments were conducted with varied N rates (0–360 kg N ha⁻¹) and three Japonica rice (Oryza sativa L.) hybrids, Lingxiangyou-18 (LXY-18), Wuxiangjing-14 (WXJ-14) and Wuyunjing (WYJ) in Jiangsu province of east China. Five hills from each plot were sampled from active tillering to heading for growth analysis and leaf N determination. The N_c dilution curve on leaf N concentration was described by the equation N_c = 3.76W^−0.218, when L_DM ranged from 0.67 to 4.25 t ha⁻¹. However, for L_DM < 0.67 t ha⁻¹, the constant critical value N_c = 4.09%L_DM was applied. This N_c dilution curve on L_DM basis was slightly higher than the curves on plant DM basis in Japonica rice, yet both lower than the reference curve of high yielding Indica rice in tropics. The N nutrition index (NNI) and accumulated N deficit (N_and) of leaves ranged from 0.65 to 1.06 and 79.62 to −6.39 kg ha⁻¹, respectively, during main growth stages under varied N rates in 2010 and 2011. The results indicate that the present N_c dilution curve and derived NNI and N_and adequately identified the situations of N-limiting and non-N-limiting nutrition in two rice varieties and could be used as reliable indicators of N status during growth of Japonica rice in east China.     

Hay yield and nitrogen harvest in smooth bromegrass mixtures with alfalfa and red clover in relation to nitrogen application

An experiment was conducted in order to investigate hay yield and nitrogen harvest in binary smooth bromegrass (Bromus inermis Leyss cv. Tohum Islah) mixtures with alfalfa (Medicago sativa L. cv. Kayseri) and red clover (Trifolium pratense L. cv. Tohum Islah) in Erzurum, Turkey for 5 years between 1991 and 1995. The Hay yield, nitrogen harvest, protein concentration and land equivalent ratio (LER) in the mixtures with alternating rows of 1:1, 2:1 and 1:2 of smooth bromegrass with alfalfa and red clover were compared to those in pure legume stands without any N-fertilizer application or pure smooth bromegrass stands that received 0, 50, 100 and 150 kg ha⁻¹ N. The mixtures had no N fertilization apart from 40 kg N ha⁻¹ in the establishment year. The dry matter production in all the mixtures receiving no N fertilizer application was higher than in pure legume stands. Pure grass stands were sustained only with the application of 150 kg ha⁻¹ N. The highest hay yields were obtained from the mixtures of smooth bromegrass (Sb) with red clover (Rc) (2Rc 1Sb) (14.65 t ha⁻¹) and with alfalfa (A) (1A 1 Sb) (14.49 t ha⁻¹). Although N application increased Sb yields in pure stands, the highest yields obtained with N fertilization were still lower than the yields in the mixtures without N application. The superiority of the mixtures was also reflected by their large N harvests (e.g. 355.9 kg N ha⁻¹ in 2Rc 1Sb plots) compared to pure Rc (317.8 kg N ha⁻¹), pure A (294.3 kg N ha⁻¹) and pure Sb stands that received 150 kg N ha⁻¹. The nitrogen harvest increased in pure Sb plots as the N doses applied increased. Furthermore, the protein concentration of the hay from the mixtures (158.2–165.7 mg g⁻¹) was equal to that of the pure A stands (165.7 mg g⁻¹) and higher than that of pure Sb stands (122.9 mg g⁻¹ at 150 kg N ha⁻¹ application) although the hay from pure Rc plots had the highest protein concentration (179.3 mg g⁻¹). The LER values were also higher in the mixtures (e.g. 1.28 in 1A 1Sb and 1.28 in 2Rc 1Sb plots) compared with the pure stands. The mixture plots also had a more balanced temporal distribution of hay. The grass component was more productive in early spring, whereas the legume fractions grew better in the summer. In conclusion, for a sustainable production of high-quality hay and greater N harvests without using N fertilizers, binary mixtures of Sb with A in alternating rows (1A 1Sb) were recommended for long-purpose stands and in alternation with double red clover rows (2Rc 1Sb) for short purpose stands under similar conditions. N application could be eliminated in the grass–legume mixtures without any yield depression.     

Energy balance of winter oilseed rape (Brassica napus L.) cropping as related to nitrogen supply and preceding crop

Data from a field experiment (1995–2000) conducted on a fertile sandy loess in the Hercynian dry region of central Germany were used to determine the energy efficiency of winter oilseed rape (Brassica napus L.) as affected by previous crop and nitrogen (N) fertilization. Depending on the previous crop, winter oilseed rate was cultivated in two different crop rotations: (1) winter barley (Hordeum vulgare L.)–winter oilseed rape–winter wheat (Triticum aestivum L.), and (2) pea (Pisum sativum L.)–winter oilseed rape–winter wheat. Fertilizer was applied to winter oilseed rape as either calcium ammonium nitrate (CAN) or cattle manure slurry. The N rates applied to winter oilseed rape corresponded to 0, 80, 160 and 240 kg N ha⁻¹ a⁻¹.Results revealed that different N management strategies influenced the energy balance of winter oilseed rape. Averaged across years, the input of energy to winter oilseed rape was highly variable ranging from 7.42 to 16.1 GJ ha⁻¹. Lowest energy input occurred when unfertilized winter oilseed rape followed winter barley, while the highest value was obtained when winter oilseed rape received 240 kg N ha⁻¹ organic fertilization and followed winter barley. The lowest energy output (174 GJ ha⁻¹), energy from seed and straw of winter oilseed rape, was observed when winter oilseed rape receiving 80 kg N ha⁻¹ as organic fertilizer followed winter barley. The energy output increased to 262 GJ ha⁻¹ for winter oilseed rape receiving 240 kg N ha⁻¹ as mineral fertilizer followed pea. The energy efficiency was determined using the parameters energy gain (net energy output), energy intensity (energy input per unit grain equivalent GE; term GE is used to express the contribution that crops make to the nutrition of monogastric beings), and output/input ratio. The most favourable N rate for maximizing energy gain (250 GJ ha⁻¹) was 240 kg N ha⁻¹, while that needed for minimum energy intensity (91.3 MJ GE⁻¹) was 80 kg N ha⁻¹ and for maximum output/input ratio (29.8) was 0 kg N ha⁻¹.     

Evaluation of nitrogen management in maize cultivation grows on soil amended with sewage sludge and urea

This paper analyses the data of a 3 years’ research on the agronomical use of sewage sludge, from a urban waste water plan, to grow maize (Zea mays L.). The experiment was conducted in order to test possible combinations of sewage sludge and urea as source of nitrogen for maize. The experiment comprised a randomized block design composed of a control and 8 treatments with four replicates. Three urea rates (0; 100 kg N ha⁻¹ and 200 kg N ha⁻¹) were assigned combined with three sewage sludge rates (0; 5 t ha⁻¹ and 10 t ha⁻¹), exceeding the limits permitted by the law, and the unfertilized control. Maize was sown and harvested for 2 years (April–September 2006 and 2007) and wheat (Triticum vulgare L.) was sown in October 2007 and harvested in May 2008 without adding any fertilizer or sewage sludge, in order to evaluate the residual effects of the organic fertilizer.The batch that gave the highest grain production was the one that received 10 t ha⁻¹ DM of sewage sludge and 100 kg N ha⁻¹ from urea, reaching values of 16.17 ± 0.97 t ha⁻¹ DM in the first year and 17.52 ± 0.68 t ha⁻¹ DM in the second one, while the effect of the organic fertilization was still available where wheat was grown. ANR values showed a significant increase between the first and the second year: the average value for the treatment 3 (exclusive use of sludge in maximum dose) has shown an increase from 24.3% in 2006 to 63.4% in 2007, highlighting the effect of the sewage sludge. Yields and nitrogen uptake during and after the experiment and the nitrate losses by leachates have been evaluated: linear correlations were statistically significant, with an improvement in the second year of the trial, between yields and the nitrogen applied (R² = 0.757) and yields and the nitrogen removal rate (R² = 0.843).     

Radiation interception and radiation use efficiency of potato affected by different N fertigation and irrigation regimes

Three years of field experiments were carried out to explore the response of potato dry matter production, accumulated intercepted photosynthetic active radiation (Aipar) and radiation use efficiency (RUE) to five N levels providing 0, 60, 100, 140 and 180 kg N ha⁻¹ and three drip irrigation strategies, which were full, deficit and none irrigation. Results showed that, irrespective of years, dry matter production and Aipar were increased by prolonged N fertigation, even though N fertigation was carried out from middle to late growing season. The highest total and tuber dry matter and accumulated radiation interception in all three years were obtained when potatoes were provided with 180 kg N ha⁻¹. RUE on the other hand was not affected by N regime. Thus, increases in total dry matter production with increasing N levels were essentially caused by higher Aipar. The strongest response to N fertilization occurred when most N was applied early in the growing season and the latest N fertilization should be applied no later than 41–50 days after emergence. Deficit irrigation, which received ca.70% of irrigation applied to full irrigation, did not reduce radiation interception and radiation use efficiency.     

Organic and mineral fertilization management improvements to a double-annual cropping system under humid Mediterranean conditions

The efficient use by crops of nitrogen from manures is an agronomic and environmental issue, mainly in double-annual forage cropping systems linked to livestock production. A six-year trial was conducted for a biennial rotation of four forage crops: oat-sorghum (first year) and ryegrass-maize (second year) in a humid Mediterranean area. Ten fertilization treatments were introduced: a control (without N); two minerals equivalent to 250 kg N ha⁻¹ year⁻¹ applied at sowing or as sidedressing; dairy cattle manure at a rate of 170, 250 and 500 kg N ha⁻¹ year⁻¹ and four treatments where the two lowest manure rates were supplemented with 80 or 160 kg mineral N ha⁻¹ year⁻¹. They were distributed according to a randomized block design with three blocks. The highest N mineral soil content was found in the summer of the third rotation, in plots where no manure was applied. The yearly incorporation of manure reduced, in successive cropping seasons, the amount of additional mineral N needed as sidedressing to achieve the highest yields. Besides, in the last two years, there was no need for mineral N application for the manure rate of 250 kg N ha⁻¹ year⁻¹. This amount always covered the oat-sorghum N uptake. In the ryegrass-maize sequence uptakes were as high as 336 kg N ha⁻¹ year⁻¹. In the medium term, the intermediate manure rate (250 kg N ha⁻¹ year⁻¹) optimizes nutrient recycling within the farming system, and it should be considered in the analysis of thresholds for N of organic origin to be applied to systems with high N demand.     

In field non-invasive sensing of the nitrogen status in hybrid bermudagrass (Cynodon dactylon × C. transvaalensis Burtt Davy) by a fluorescence-based method

The level of N fertilization and the content of leaf N in Cynodon dactylon × C. transvaalensis Burtt Davy cv. ‘Tifway 419’ bermudagrass were evaluated non-destructively with a fluorescence-based method. It was applied directly into the field by using the Multiplex portable fluorimeter during two consecutive seasons (2010 and 2011). In the 2010 experiment, the nitrogen balance index (NBI₁) provided by the sensor was able to discriminate (at P < 0.05) six different N levels applied, up to 250 kg ha⁻¹, with a precision (root mean square error, RMSE) in the rate estimate of 3.29 kg ha⁻¹. In 2011, the index was insensitive to the N treatment between 150 kg ha⁻¹ and 250 kg ha⁻¹ N rates, and its precision was 39.98 kg ha⁻¹. Calibration of the sensor by using the destructive analysis of turf samplings showed a good linear regression between NBI₁ and the leaf N content for both 2010 (R² = 0.81) and 2011 (R² = 0.93) experiments. This allowed mapping of the leaf N spatial distribution acquired by the sensor in the field with a prediction error of 0.21%. Averaging the overall estimates of leaf N content per N treatment provided an upper limit of 200 kg ha⁻¹ for the required fertilization, corresponding to a critical level of leaf N of about 2.3%. Our results confirm the usefulness of the new fluorescence-based method and sensor for a precise management of fertilization in turfgrass.     

Influence of harvest time and frequency on light interception and biomass yield of festulolium and tall fescue cultivated on a peatland

In this study, we report efficiencies of light capture and biomass yield of festulolium and tall fescue cultivated on a riparian fen in Denmark under different harvesting managements. Green biomass targeted for biogas production was harvested either as two cuts (2C) or three cuts (3C) in a year. Three different timings of the first cut in the 2C systems were included as early (2C-early), middle (2C-mid) and late (2C-late) cuts corresponding to pre-heading, inflorescence emergence and flowering stages, respectively. The fraction of intercepted photosynthetically active radiation (f_PAR) was derived from the canopy reflectance measured on 61 dates throughout a year, and cumulative interception of PAR (IPAR) and radiation use efficiency (RUE) was calculated. The dynamics of f_PAR and biomass accumulations was similar for both crops before the first cuts in all managements. Festulolium f_PAR in 2C-early and 2C-mid managements declined faster than in 2C-late and 3C managements in the second growth period and thus growing period IPAR of 2C-early and 2C-mid declined by 8% as compared to 3C management where IPAR was 925 MJ m⁻². Annual festulolium dry matter (DM) yield in 2C-early and 2C-mid managements (average 14.1 Mg DM ha⁻¹) decreased by 22% compared to 3C management (18.2 Mg DM ha⁻¹). The highest and the lowest RUE of festulolium were observed in 3C and 2C-mid managements as 1.97 and 1.59 g MJ⁻¹, respectively. For tall fescue f_PAR declined rather slowly in the second growing period in all 2C managements, which contributed to similar IPAR (908–919 MJ m⁻²), total biomass yield (16.4–18.8 Mg DM ha⁻¹ yr⁻¹) and RUE (1.80–2.07 g MJ⁻¹) for all managements. Whereas both crops were highly productive under both 3C management and 2C management with first harvest after flowering (i.e., 2C-late), the 2C-late strategy is recommended as the least intensive of the two management systems.