Morphometric traits and iPBS based molecular characterizations of walnut (Juglans regia L.) genotypes

Genetic Resources and Crop Evolution - In this study, walnut genotypes that were selected during two growing seasons among thousands of seedlings were analyzed in terms of detailed morphometric,...     

Exploring low-chill genotypes of Persian walnut (Juglans regia L.) in west of Iran

Genetic Resources and Crop Evolution - Climate change may cause a significant decline in winter chill over the coming decades and is becoming an important issue for walnut (Juglans regia L.)...     

Polyphenolic profiles and antioxidant activities of heartnut (Juglans ailanthifolia Var. cordiformis) and Persian walnut (Juglans regia L.)

The polyphenolic compositions of three heartnut (Juglans ailanthifolia var. cordiformis) varieties (Imshu, Campbell CW1, and Campbell CW3) were examined and compared with those of two Persian walnut (Juglans regia L.) varieties (Combe and Lake). The nuts were defatted, extracted, and separated into three different fractions, the free phenolic acid (FPA), acid-hydrolyzable phenolic acid (AHPA), and bound phenolic acid (BPA) fractions. The total phenolic contents (TPCs) in both FPA and AHPA of the Persian walnuts were significantly higher (P < 0.001) than those of the heartnuts, but not in the BPA (P = 0.20). LC-ESI-MS(n)() studies revealed that except for the FPA fraction, the major polyphenolics in both heartnut and Persian walnut were ellagic acid and valoneic acid dilactone. Persian walnuts contained an average of 0.29 and 1.31 mg of ellagic acid/g nut in the 80% methanol extractable fractions FPA and AHPA, respectively. Heartnuts contained an average of 0.16 and 0.60 mg of ellagic acid/g nut in the respective fractions. Bound ellagic acid in the residue was 0.93 and 0.70 mg/g of nut in the Persian walnut and in the heartnut, respectively. Valoneic acid dilactone was tentatively identified and quantified as milligrams of ellagic acid equivalent per gram of nut. These components were found to contribute to the strong total antioxidant activities measured using ferric reducing antioxidant power and photochemiluminescence methods.     

Phenological and pomological properties of promising walnut (Juglans regia L.) genotype with cluster fruiting from selected native population in Oltenia,Romania

Genetic Resources and Crop Evolution - Lateral fruiting in walnut is an important feature and many breeding programs follow this criterion. Following the evaluation of walnut genotypes from Padea...     

Fatty acid profiles, tocopherol contents, and antioxidant activities of heartnut (Juglans ailanthifolia Var. cordiformis) and Persian walnut (Juglans regia L.)

The fatty acid and tocopherol compositions of three heartnut (Juglans ailanthifolia var. cordiformis) varieties (Imshu, Campbell CW1, and Campbell CW3) were examined and compared with those of two Persian walnut (Juglans regia L.) varieties (Combe and Lake). The major fatty acids found in heartnuts and walnuts were identified by gas chromatography as linoleic (18:2n-6), alpha-linolenic (18:3n-3), oleic (18:1n-9), palmitic (16:0), and stearic acid (18:0). Polyunsaturated fatty acids were the main group of fatty acids found in both heartnut and walnut, ranging from 73.07 to 80.98%, and were significantly higher in heartnut than in Persian walnuts (P < 0.001). In addition, heartnuts had significantly higher levels of 18:2n-6 and lower levels of 18:3n-3 compared to the Persian walnuts. gamma-Tocopherol was the main tocopherol homologue present in both types of nuts, followed by delta- and alpha-tocopherol. The highest concentration of gamma-tocopherol was found in Combe Persian walnut at 267.87 mug/g, followed by Lake Persian walnut and Imshu, Campbell CW1, and CW3 heartnut at 205.45, 187.33, 161.84, and 126.46 mug/g, respectively. Tocopherols, particularly the gamma-tocopherol, were found to contribute the most to the strong total antioxidant activities of both walnut and heartnut oils using either the free radical 2,2-diphenyl-1-picrylhydrazyl assay or the photochemiluminescence method.     

Triacylglycerol composition of walnut (Juglans regia L.) cultivars: characterization by HPLC-ELSD and chemometrics

A total of 26 walnut (Juglans regia L.) samples from 9 cultivars (Arco, Franquette, Hartley, Lara, Marbot, Mayette, Mellanaise, Parisienne, and Rego) harvested in the 2001, 2002, and 2003 crop years and grown in two geographical origins (Braganca and Coimbra, Portugal) were evaluated with regard to their triacylglycerol composition. The methodology employed was reversed-phase high-performance liquid chromatography coupled to an evaporative light-scattering detector (RP-HPLC-ELSD) after extraction of the lipidic fraction of the nuts. Nine compounds were separated, identified, and quantified. All samples presented an identical qualitative profile composed by LLnLn, LLLn, LLL, OLLn, OLL, PLL, OOL, and PLO (P = palmitoyl; O = oleoyl; L = linoleoyl; Ln = linonenoyl). Trilinolein (LLL) was the major triglyceride, followed by dilinoeoyl-oleoyl-glycerol (OLL) and dilinoleoyl-linolenoyl-glycerol (LLLn), with mean values of 37.7, 18.5, and 18.4%, respectively. Significant differences in composition were found between cultivars, and these differences were also significant when cultivars were grouped by year of production, showing that besides genetic factors, the triacylglycerol composition can be strongly influenced by environmental factors.     

Determining phenological and genetic variation in genotypes obtained from open-pollinated seeds of ‘Maraş 12’ walnut (Juglans regia L.) cultivar

Genetic Resources and Crop Evolution - In the present study, the phenological and genetic differences/similarities of 95 walnut genotypes were considered. These genotypes had been propagated...     

Determination of sterol and fatty acid compositions, oxidative stability, and nutritional value of six walnut (Juglans regia L.) cultivars grown in Portugal

Six cultivars (Franquette, Marbot, Mayette, Mellanaise, Lara, and Parisienne) of walnuts (Juglans regia L.) were collected during the 2001 crop, from Bragan?a, Portugal. Chemical composition, including moisture, total oil content, crude protein, ash, carbohydrates, and nutritional value, was evaluated. Fat was the predominant component, ranging from 62.3 to 66.5%. Total oil was extracted and analyzed for fatty acids, sterols, oxidative stability, and peroxide value. Fatty acids and sterols were determined by gas-liquid chromatography coupled to a flame ionization detector. Eighteen fatty acids were quantified. Polyunsaturated fatty acids and, in particular, linoleic acid were predominant. Beta-Sitosterol, delta(5)-avenasterol, and campesterol were the major sterols found. Differences were observed among the studied cultivars, especially in peroxide values and in the sterol profile.     

Response of selected drought tolerant wheat (Triticum aestivum L.) genotypes for agronomic traits and biochemical markers under drought-stressed and non-stressed conditions

ABSTRACT

Genetic improvement of wheat for drought tolerance can be achieved by developing suitable ideotypes with enhanced yield-response associated with agronomic traits and biochemical markers. The objective of this study was to determine drought response of elite drought tolerant wheat genotypes using agronomic and biochemical traits to select promising lines for breeding. Fourteen wheat genotypes selected from the International Maize and Wheat Improvement Center’s heat and drought tolerance nursery and one standard check variety were evaluated under drought-stressed (DS) and non-stressed (NS) conditions using a randomised complete block design in three replications. Significant (P?<?0.05) genotype, drought condition and genotype?×?drought condition interaction effect were detected for the tested traits suggesting differential response of genotype for selection. Grain yield positively correlated with sucrose (r?=?0.58; P?<?0.05), fructose (r?=?0.52; P?<?0.05) and total sugar (r?=?0.52; P?<?0.05) contents under NS condition and with sucrose (r?=?0.80; P?<?0.001), total sugar (r?=?0.84; P?<?0.001) content, proline content (r?=?0.74; P?<?0.001) and number of grains per spike (r?=?0.58; P?<?0.05) under DS condition. Genetically unrelated wheat genotypes such as SM04, SM19, SM29, SM32, SM45 and SM97 possessing key agronomic and biochemical traits were selected for cultivar development for drought-stressed environments.     

Assessment of terminal drought tolerance among elite wheat (Triticum aestivum L.) genotypes using selected agronomic and physiological traits

ABSTRACT

The selection response of wheat (Triticum aestivum L.) under water-limited condition can be enhanced through breeding novel genotypes possessing drought-adaptive and yield-related agronomic and physiological traits. The objective of this study was to evaluate terminal drought tolerance among bread wheat genotypes and select superior parents for breeding. Agronomic and physiological responses of 28 wheat lines were assessed under well-watered (WW) and terminal drought (TD) treatments using a 7?×?4 alpha-lattice design under rainout shelter (RS) and glasshouse (GH) environments. Significant (p?≤?0.05) genotype?×?environment interaction effects were observed for number of days to heading (DTH), number of days to maturity (DTM), number of productive tillers per plant (TN), grain yield (GY), thousand kernel weight (TKW), stomatal conductance (SC), leaf canopy temperature (LCT) and chlorophyll content index (CCI) suggesting varied genotypic response under WW and TD treatments. Correlation analysis revealed significant associations TN and GY (r?=?0.67; p?≤?0.001), TKW and GY (r?=?0.72; p?≤?0.001), CCI and GY (r?=?0.39; p?≤?0.05) under TD treatment. Drought tolerant wheat genotypes such as LM100, LM72, LM22 and LM95 are useful for direct cultivation and for developing breeding populations with enhanced yield performance.     

Relationships among some cornelian cherry genotypes (<Emphasis Type="Italic">Cornus mas</Emphasis> L.) based on RAPD analysis

Turkey is an important producer of cornelian cherries (Cornus mas L.), especially in northern Anatolia. Seed propagation and long-term human selection has given rise to a great diversity of trees. Twenty-six cornelian cherry genotypes (CC1–CC26) from the Coruh Valley in northern Anatolia were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) markers, based on 56 decamer random primers, seven of which showed reliable polymorphisms. These seven primers generated 80 markers, with 77 (96.25%) displaying polymorphisms. Cluster analysis of the cornelian cherry genotypes was performed based on data from polymorphic RAPD bands, by using Jaccard’s similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. A similarity matrix showed that the highest genetic similarity (0.913) was between CC15 and CC16 and the least (0.129) was between CC4 and CC16. The cophenetic correlation coefficient between the similarity matrix and the cophenetic matrix of the dendrogram was relatively high (r = 0.87), supporting the validity of the dendrogram. Based on these results, RAPD analysis can be used for the characterization and grouping of cornelian cherry genotypes. Genetically divergent genotypes identified in this study may be useful for future breeding programs. This is the first study demonstrating that RAPD analyses can be used to differentiate and classify cornelian cherry genotypes.