Effects of oscillating dietary protein on nutrient digestibility,nitrogen metabolism,and gastrointestinal organ mass in sheep

Twenty-four wether lambs (BW = 37.5 +/- 0.8 kg) were used in a 64-d randomized complete block design experiment to evaluate the effect of oscillating dietary CP with undegradable intake protein (UIP) on diet digestibility, N retention, and gastrointestinal (GI) organ mass. Four treatments consisted of a 13, 15, or 17% CP diet fed daily or a regimen in which dietary CP was oscillated between 13 and 17% on a 48-h basis (ACP). All diets consisted of 65% bromegrass hay (10.5% CP, 61.9% NDF, 37.2% ADF) and 35% corn-based supplement, and were formulated to contain the same amount of degradable intake protein (9.6% of dry matter), plus additional UIP (from SoyPLUS) to accomplish CP levels above 13%. Beginning on d 52, N balance collections were conducted for 8 d, after which lambs were killed on d 62 and 64 of the trial for measurement of GI organ mass. Because intake was restricted to 3.0% of initial body weight (dry matter basis), dry matter intake did not differ (P > or = 0.67) and no treatment effects (P > or = 0.36) on ADG, feed efficiency, or total tract DM digestibility were observed. Increasing dietary CP from 13 to 17% linearly increased (P = 0.0001) N digestibility, but lambs fed ACP had lower (P = 0.07) total tract N digestibility than those fed 15% CP daily. Although urinary N excretion increased linearly (P = 0.0001) with increasing CP, a linear increase (P = 0.07) was observed in N retention (g/d) with increasing dietary CP. Although the quantity of N retained by lambs fed ACP was not statistically different (g/d, P = 0.19; % of digested N, P = 0.23) from those fed 15% CP daily, N retention in lambs fed ACP was 42% lower than in those fed 15% CP daily (1.8 vs 3.1 g/d, respectively). Increasing CP linearly decreased (P < or = 0.09) weights of the reticulorumen, abomasum, and small intestine, but did not affect (P > or = 0.16) liver or omasum weights. Length of the small intestine was not affected (P > or = 0.45) by treatment, but lambs fed ACP had greater (P = 0.03) small intestine weights than those fed 15% CP daily. Increasing dietary CP linearly decreased (P = 0.03) total GI organ mass, and lambs fed ACP had a greater (P = 0.03) total GI organ mass than those fed 15% CP daily. Oscillating dietary CP may increase the weights of the GI organs, which may subsequently have negative effects on N and energy metabolism in the animal. Likewise, the potential for decreased GI organ mass in response to increased supply of CP with UIP deserves further investigation.     

Substitution of wheat dried distillers grains with solubles for barley grain or barley silage in feedlot cattle diets: intake, digestibility, and ruminal fermentation

The objective of this study was to evaluate the effects of substituting wheat dried distillers grains with solubles (DDGS) for barley grain and barley silage on intake, digestibility, and ruminal fermentation in feedlot beef cattle. Eight ruminally cannulated Angus heifers (initial BW 455 ± 10.8 kg) were assigned to a replicated 4 × 4 Latin square design with 4 treatments: control, low (25%), medium (30%), and high (35%) wheat DDGS (DM basis). The diets consisted of barley silage, barley concentrate, and wheat DDGS in ratios of 15:85:0 (CON), 10:65:25 (25DDGS), 5:65:30 (30DDGS), and 0:65:35 (35DDGS; DM basis), respectively. The diets were formulated such that wheat DDGS was substituted for both barley grain and barley silage to evaluate whether wheat DDGS can be fed as a source of both energy (grain) and fiber in feedlot finishing diets. Intakes (kg/d) of DM and OM were not different, whereas those of CP, NDF, ADF, and ether extract (EE) were greater (P < 0.01) and intake of starch was less (P < 0.01) for the 25DDGS compared with the CON diet. The digestibilities of CP, NDF, ADF, and EE in the total digestive tract were greater (P < 0.05) for 25DDGS vs. CON. Ruminal pH and total VFA concentrations were not different (P > 0.15) between 25DDGS and CON diets. Replacing barley silage with increasing amounts of wheat DDGS (i.e., from 25DDGS to 35DDGS) linearly reduced (P < 0.05) intakes of DM and other nutrients without altering (P=0.40) CP intake. In contrast, digestibilities of DM and other nutrients in the total digestive tract linearly increased (P < 0.05) with increasing wheat DDGS except for that of EE. Additionally, with increasing amounts of wheat DDGS, mean ruminal pH tended (P=0.10) to linearly decrease, and ruminal pH status decreased with longer (P=0.04) duration of pH <5.5 and <5.2, and greater (P=0.01) curve area under pH <5.8 and <5.5 without altering (P > 0.19) ruminal VFA and NH(3)-N concentrations. Results indicated that wheat DDGS can be effectively used to replace both barley grain and silage at a moderate amount to meet energy and fiber requirements of finishing cattle. However, when silage content of the diet is low (<10%), wheat DDGS is not an effective fiber source, so replacing forage fiber with wheat DDGS in finishing diets decreases overall ruminal pH status even though the rapidly fermentable starch content of the diet is considerably reduced.     

Winter-feeding systems for gestating sheep II. Effects on feedlot performance, glucose tolerance, and carcass composition of lamb progeny

Mature pregnant crossbred ewes (n = 90) were used in a randomized complete block design experiment and were assigned to 1 of 3 winter-feeding systems differing in primary feed source: haylage (HL), limit-fed corn (CN), or limit-fed dried distillers grains (DDGS). Effects of these winter-feeding strategies on postweaning progeny performance were determined. Lamb progeny (n = 96) were weaned at 61 ± 4 d of age and fed a common high-concentrate diet. Lambs were assigned to feedlot pen (n = 18) based on dam mid-gestation pen. Growth rate, DMI, and ADG were determined for the first 40 d of the finishing period. At 96 ± 4 d of age, 1 wether lamb was randomly selected from each pen (n = 18) for a glucose tolerance test. The experiment was terminated, and lambs were slaughtered individually when they were determined to have achieved 0.6-cm 12th-rib fat thickness. After a 24-h chill, carcass data were collected and a 2.54-cm chop was removed from each lamb from the LM posterior to the 12th rib for ether extract analysis. Additional carcass measurements of bone, muscle, and fat from the shoulder, rack, loin, and leg were collected on 35 carcasses. At weaning, lamb BW was not different among treatments, whereas final BW tended to be greater (P = 0.09) for lambs from ewes fed DDGS and CN during gestation than from those fed HL. Overall lamb growth rate from birth to slaughter was not different among treatments. Lambs from ewes fed DDGS vs. CN or HL tended to have a greater initial insulin response (P = 0.09). Dressing percent was less (P = 0.04) in lambs from ewes fed DDGS, but no difference (P = 0.16) was detected in HCW among treatments. As expected, 12th rib fat thickness was similar among treatments, whereas LM area was largest to smallest (P = 0.05) in lambs from ewes fed CN, HL, and DDGS, respectively. Proportion of internal fat tended to be greatest to smallest (P = 0.06) in lambs from ewes fed DDGS, CN, and HL, respectively. Calculated boneless trimmed retail cuts percentage was less (P = 0.04) in lambs from ewes fed DDGS than CN or HL. Loin muscle weight as a percentage of wholesale cut tended (P = 0.10) to be greater in lambs from ewes fed CN and HL than DDGS, whereas other muscle, bone, and fat weights and proportions were similar (P > 0.24) among treatments. Prepartum diet during mid to late gestation of ewes altered postnatal fat and muscle deposition and may be associated with alterations in insulin sensitivity of progeny.     

Effect of whole-crop pea (Pisum sativum L.) silages differing in condensed tannin content as a substitute for grass silage and soybean meal on the performance, metabolism, and carcass characteristics of lambs

Two experiments were conducted to investigate the effect of inclusion of whole-crop pea (WCP) silages, differing in condensed tannin content, as a substitute for grass silage (GS) and soybean meal on lamb metabolism, performance, plasma metabolites, digestibility, and carcass characteristics. In both experiments lambs were offered either solely GS or a 50:50 mix on a DM basis of GS with either low-tannin (LTPS) or high-tannin (HTPS) pea silage ad libitum. Each forage mix was fed with either 400 g/d of low-protein (LP) concentrate or 400 g/d of LP with an additional 200 g/d of pelletized soybean meal (HP), resulting in 6 dietary treatments. Experiment 1 examined the effects of the diets on metabolism, digestibility, and N balance using 6 lambs in 4 periods of 21 d in an incomplete crossover design. Experiment 2 used 48 lambs and examined the effects of the diets on ADG, plasma metabolites, and carcass characteristics over 56 d. Both experiments were analyzed using a 3 × 2 factorial arrangement of treatments. In Exp. 1, lambs offered the LTPS diets had a greater (P < 0.05) digestibility of DM and OM than those offered the GS diets. Lambs offered the WCP silages had an increased (P < 0.05) N intake, N output, and digestibility of GE compared with those offered GS. Mean N digestibility was greatest (P < 0.05) in lambs offered LTPS. Lambs offered HP diets had increased (P < 0.001) digestibility of DM, OM, GE and N, and N- intake, output, retention, and digestibility compared with those offered the LP diets. In Exp. 2, there was no effect (P > 0.05) of forage type on intake, slaughter BW, or feed conversion efficiency (FCE). However, lambs offered the LTPS had a greater (P < 0.05) ADG than those offered the GS diets. Feeding diets containing HP increased (P < 0.001) total DMI, slaughter BW, ADG, and FCE. Lambs offered the WCP had a greater (P < 0.05) plasma β-hydroxybutyrate and urea concentration compared with those offered the GS diets. Feeding lambs HP diets increased (P < 0.05) plasma urea and total protein. Forage mix had no effect (P > 0.05) on carcass composition except for fat depth, which was greater (P < 0.05) in lambs offered WCP silage. Diets containing the HP increased (P < 0.05) carcass weight, hind leg circumference, chop dimensions, and kidney weight. It was concluded that lambs offered LTPS performed better than those offered GS and that LTPS has a concentrate sparing effect. Additionally, the increased tannin concentration in HTPS did not increase performance over lambs offered either GS or LTPS.     

Organ weight, intestinal morphology, and fasting whole-body oxygen consumption in growing pigs fed diets containing distillers dried grains with solubles alone or in combination with a multienzyme supplement

The effects of adding a multienzyme complex to a diet containing distillers dried grains with solubles (DDGS) produced from a 1:1 mixture of corn and wheat on visceral organ weight, intestinal morphology, and fasting whole-body oxygen consumption (FWBOC) were investigated in growing pigs in a 28-d trial. Twenty-four pigs (BW = 19.9 ± 0.5 kg) were individually housed in floor pens and randomly assigned to 3 experimental diets (8 pigs per diet). The diets contained corn and soybean meal with 0% (control) or 30% DDGS (DDGS diet); the third diet was supplemented with a multienzyme complex in addition to the 30% DDGS (DDGS + enzyme diet). All diets had similar nutrient concentrations and met the 1998 NRC nutrient requirements for growing pigs. Pigs were fed at 4% of their BW once daily. On d 15, 4 pigs from each dietary treatment were randomly selected for measurement of FWBOC during the 24- to 30-h postprandial period using an open-circuit indirect calorimeter. At the end of the study, pigs were killed to determine visceral organ weights, ileal and cecal digesta viscosity, and intestinal morphology. There was no effect (P > 0.05) of dietary treatment on final BW, WBFOC, or digesta viscosity. Empty BW of pigs fed the control diet was heavier (P = 0.02) than that of pigs fed the DDGS diet, but the empty BW of pigs fed the DDGS + enzyme diet was not different (P > 0.05) from that of pigs fed the control or DDGS diet. There were no differences (P > 0.05) in empty BW of liver, spleen, pancreas, heart, stomach, small intestine, and cecum among dietary treatments on a per kilogram basis. However, pigs fed the DDGS diet had heavier (P < 0.05) colon plus rectum and portal-drained viscera (PDV) than pigs fed the control diet, but weights of colon plus rectum and PDV in pigs fed the DDGS + enzyme diet were not different (P > 0.05) from those of pigs fed the control diet. Although morphological data showed no differences (P > 0.05) in the duodenum, jejunum, and colon segments among dietary treatments, the DDGS diet tended to decrease (P < 0.10) villous height and villous height to crypt depth in the ileum. The results of this experiment indicated that pigs fed a diet containing 30% DDGS have reduced dressing percentage and increased visceral organ mass compared with pigs fed a corn-soybean meal diet. However, the addition of a multienzyme complex to the DDGS diet resulted in pigs having a dressing percentage and visceral organ mass that are not different from those of pigs fed a corn-soybean meal diet.     

Effects of dietary n-6:n-3 fatty acid ratio on feed intake, digestibility, and fatty acid profiles of the ruminal contents, liver, and muscle of growing lambs

This study investigated the effect of modifying the n-6:n-3 fatty acid ratio (FAR) of diets using linseed, soybean, and cottonseed oils on apparent digestibility, ruminal fermentation characteristics, growth performance, key circulating hormones, and the fatty acid profile of ruminal digesta, liver, and fore-shank muscle of growing lambs fed a high concentrate diet. Forty individually housed Katadhin Dorper lambs (average of 20.0 kg of BW) were fed Bermudagrass hay in ad libitum amounts and concentrates at 3.7% of BW daily. The concentrate contained 68.9% corn, 23.8% soybean meal, 3.3% limestone, and 4.0% oil supplements (DM basis). The treatments consisted of dietary n-6:n-3 FAR of 2.3:1, 8.8:1, 12.8:1, and 15.6:1. After feeding for 35 d in metabolism crates, lambs were slaughtered 15 h after feeding, and samples of ruminal digesta, blood, liver, and foreshank tissue were collected. Increasing dietary n-6:n-3 FAR did not affect the intake of DM nor the apparent digestibility of DM, ether extract, NDF, or ADF, but did increase apparent digestibility of CP (linear, P < 0.05). Concentrations of ruminal butyrate increased linearly (P < 0.05) with increasing dietary n-6:n-3 FAR, whereas the valerate concentration decreased linearly (P < 0.001). Concentrations of plasma insulin and IGF-I were not affected by dietary n-6:n-3 FAR. Concentrations of C18:3n-3 increased linearly (P < 0.001), whereas that of C18:2n-6 decreased linearly (P < 0.001) in ruminal digesta with decreasing dietary n-6:n-3 FAR. Concentrations of transisomers of fatty acids in ruminal digesta did not change. Proportions of C18:0 in liver and foreshank muscle were unchanged by diet. The proportion of trans11 C18:1 and cis-9 trans11 CLA decreased (P < 0.05) in liver but increased (P < 0.05) in foreshank muscle as dietary n-6:n-3 FAR decreased. Proportions of all measured n-3 fatty acids were greater in liver when diets contained more C18:3n-3 from linseed oil. By decreasing the dietary n-6:n-3 FAR, the proportions of n-6 fatty acids in foreshank muscle decreased dramatically; specifically, C18:2n-6 decreased linearly (P < 0.001) from 28.0 to 16.5% and C20:4n-6 decreased linearly (P < 0.001) from 14.7 to 8.6%. Although feeding a diet that contained more n-3 fatty acids increased the n-3 fatty acid concentration of muscle, the ratio of PUFA to SFA was decreased.     

Digestibility and dry matter intake of diets containing alfalfa and kenaf

Two experiments were conducted to determine the dietary value of pellets containing kenaf (Hibiscus cannabinus cv. 'Everglade 41') hay. Averaged across both experiments, kenaf pellets contained 82.6% kenaf hay, 16.6% liquid molasses, and 0.8% mineral oil. The chemical composition of the kenaf pellet was 12.6% crude protein (CP), 41.2% neutral detergent fiber (NDF), and 14.4% acid detergent fiber (ADF). In Exp. 1 (digestion and N balance trial), 18 lambs (body weight [BW] = 36.4 kg) were blocked by BW. Lambs were randomly assigned within a block to Diet 1 (59.5% corn and 40.5% alfalfa pellet), Diet 2 (59.7% corn, 28.4% alfalfa pellets, and 11.9% kenaf pellets), or Diet 3 (59.6% corn, 16.5% alfalfa pellets, and 23.9% kenaf pellets). Diets were formulated so that CP was the first-limiting nutrient. Each diet was limit-fed at 2.4% of BW. Replacing alfalfa pellets with kenaf pellets tended to decrease (P = 0.10) CP and ADF intakes, but increased (P = 0.01) DM digestibility. Diet had no effect (P = 0.33) on N balance. In Exp. 2 (dry matter [DM] intake trial), 32 lambs (BW = 30.4 kg) were blocked by gender and BW. Within a block, lambs were randomly assigned to one of four diets in a 2 x 2 factorial arrangement. Main effects were hay (bermudagrass or fescue) and supplemental protein source (kenaf or alfalfa pellets). Lambs were housed in individual pens with ad libitum access to the assigned hay. Supplemental protein was fed (185 g of DM) once daily. Hay intake was measured weekly for 8 wk. Lambs consumed more (P = 0.002) fescue than bermudagrass hay (743 vs 621 g/ d). Lambs fed fescue hay gained weight more rapidly (P = 0.001) than lambs fed bermudagrass hay (120 vs 72 g/d). Hay intake and ADG were similar (P = 0.90) for lambs fed alfalfa or kenaf pellets. Kenaf hay mixed with molasses and mineral oil can be formed into a pellet. In the diets used in this experiments, kenaf pellets can replace alfalfa pellets in diets fed to lambs without altering forage intake, gain, or N retention.     

Comparison of wheat or corn dried distillers grains with solubles on rumen fermentation and nutrient digestibility by feedlot heifers

A 5 × 5 Latin square design trial was conducted to evaluate rumen fermentation and apparent nutrient digestibility in 5 rumen-cannulated heifers (420 ± 6 kg) fed a barley-based finishing diet supplemented with 20 or 40% wheat or corn dried distillers grains with solubles (DDGS). The composition of the control diet was 88.7% rolled barley grain, 5.5% supplement, and 5.8% barley silage (DM basis). Increasing the quantity of corn DDGS in the ration resulted in a quadratic decrease in DMI (P = 0.04) and OM intake (P = 0.05). Rumen pH, pH duration, and area under rumen pH thresholds of 5.8 or 5.5 were not affected (P > 0.05) by treatment. Inclusion of wheat DDGS resulted in a quadratic increase (P = 0.05) in pH area below the cutoff value of 5.2, with the most pronounced effect at 20% inclusion. Wheat DDGS linearly increased (P = 0.01) rumen NH(3)-N concentrations. Increasing the inclusion rate of wheat and corn DDGS resulted in quadratic (P = 0.05) and linear (P = 0.04) decreases in rumen propionate, whereas butyrate increased quadratically (P < 0.01) and linearly (P < 0.01), respectively. Feeding wheat DDGS linearly decreased (P < 0.01) DM and OM digestibility values. Inclusion of corn DDGS increased the digestibility values of ether extract (P = 0.05; quadratic response) and CP (P < 0.01; linear response). Neutral detergent fiber digestibility increased in a linear fashion (P = 0.01) as both wheat and corn DDGS inclusion increased, whereas ADF digestibility increased linearly (P = 0.03) for wheat and quadratically (P = 0.02) for corn DDGS. Increased inclusion of wheat DDGS resulted in a linear decrease in GE digestibility (P = 0.01), whereas increasing corn DDGS inclusion linearly increased (P < 0.01) the DE content of the diet. Feeding both wheat and corn DDGS linearly increased (P = 0.01) the excretion of N and P. In summary, replacement of barley grain with up to 40% wheat or corn DDGS did not mitigate rumen pH conditions associated with mild to moderate acidosis in heifers fed a barley-based finishing diet. Supplementing corn DDGS increased nutrient digestibility of all nutrients and, as a result, led to greater DE content. Supplementation of wheat DDGS reduced DM and OM digestibility values, with no effect on DE content. Increased N and P excretion by heifers fed DDGS at 20 or 40% of dietary DM presents a challenge for cattle feeders with respect to nutrient management.     

Growth performance and carcass characteristics of grower-finisher pigs fed high-quality corn distillers dried grain with solubles originating from a modern Midwestern ethanol plant

A growth performance and carcass evaluation study was conducted to determine the maximal inclusion rate of corn distillers dried grain with solubles (DDGS) in grower-finisher pig diets when formulated on a total AA basis. A total of 240 (28.4 +/- 0.8 kg of BW) crossbred pigs [(Yorkshire x Landrace) x Duroc] were allotted randomly within sex and weight outcome groups to 1 of 24 pens. Pens were assigned randomly within the initial BW groups to 1 of 4 dietary treatment sequences in a 5-phase grower-finisher feeding program in a 4 x 3 factorial arrangement of treatments. The inclusion level of DDGS (0, 10, 20, or 30%) in the diet and the initial BW class [low (23.2 kg), medium (28.1 kg), or high (33.8 kg)] served as the main factors for the grower-finisher performance study. All diets were formulated to contain similar concentrations of total Lys, ME, calcium, and phosphorus within each phase. Pigs were slaughtered and carcass data were collected when the average BW of pigs in a pen reached 114 +/- 2.25 kg. Dietary treatment and initial weight groups did not interact for any response variables, and only the main effects of dietary treatment are presented. Pigs fed the 20 or 30% DDGS diets had reduced ADG (P < 0.05) compared with that of the 0 or 10% DDGS groups, but ADFI was unaffected by dietary treatment. Gain:feed decreased when pigs were fed 30% DDGS (P < 0.05) compared with the 0, 10, and 20% DDGS dietary inclusion levels. Loin depth was lower in pigs fed the 30% DDGS diets (P < 0.05), but backfat depth and percentage of carcass lean did not differ among treatments. Iodine number of carcass fat increased linearly (P < 0.01) with increasing dietary DDGS concentration, and belly firmness adjusted for belly thickness was reduced (P < 0.05) for pigs fed the 30% DDGS diets compared with pigs fed the 0 or 20% DDGS diets. Color measurements, ultimate pH, and visual evaluations (color, firmness, and marbling scores) of the LM did not differ among treatments. Cooking loss, 24-h drip loss, and total moisture loss were not affected by DDGS in the diets. However, differences were detected between 0 and 20% DDGS treatments for 11-d purge loss (P < 0.05). Dietary treatment did not affect Warner-Bratzler shear force of cooked loin chops. Results from this study indicate that when diets for grower-finisher pigs are formulated on a total AA basis, less than 20% DDGS should be included in the diet for optimal performance and carcass composition. Feeding DDGS in swine finishing diets did not have any detrimental effects on pork muscle quality.     

Assessment of energy content of low-solubles corn distillers dried grains and effects on growth performance, carcass characteristics, and pork fat quality in growing-finishing pigs

Two studies were conducted to assess the energy content of low-solubles distillers dried grains (LS-DDG) and their effects on growth performance, carcass characteristics, and pork fat quality in grow-finish pigs. In Exp. 1, 24 barrows (Yorkshire-Landrace × Duroc; 80 to 90 d of age) in 2 successive periods were assigned to 1 of 6 dietary treatments. In individual metabolism stalls, pigs were fed a corn-soybean meal diet (control); control replaced by 30, 40, or 50% LS-DDG; or control replaced by 30 or 40% distillers dried grains with solubles (DDGS) at 3% of their initial BW for 12 d. All diets contained 0.25% CrO(2). During the 5-d collection period, feces and urine were collected from each pig. Feed, feces, and urine were analyzed for DM, GE, and N concentrations, and feed and feces were analyzed for Cr content. The ME content of LS-DDG (2,959 ± 100 kcal/kg of DM) was similar to that determined for DDGS (2,964 ± 81 kcal/kg of DM). In Exp. 2, 216 Yorkshire-Landrace × Duroc pigs were blocked by initial BW (18.8 ± 0.76 kg) and assigned to 1 of 24 pens (9 pigs/pen). Pens within block were allotted to 1 of 3 dietary treatments (8 pens/treatment) in a 4-phase feeding program: a corn-soybean meal control (control), control containing 20% LS-DDG, or control containing 20% DDGS. Treatment had no effect on final BW, ADG, ADFI, or HCW. Pigs fed LS-DDG had similar G:F (0.367) compared with pigs fed DDGS (0.370), but tended (P = 0.09) to have decreased G:F compared with pigs fed the control (0.380; pooled SEM = 0.004). Dressing percent was less (P < 0.01) for pigs fed LS-DDG (72.8%) and DDGS (72.8%) compared with the control (73.8%; pooled SEM = 0.22). Pigs fed LS-DDG (54.8%) had greater (P = 0.02) carcass lean compared with pigs fed DDGS (53.4%), but were similar to pigs fed control (54.1%; pooled SEM = 0.33). Bellies from pigs fed DDGS (12.9°) were softer (P < 0.01) than those from pigs fed control (17.7°; pooled SEM = 1.07) as determined by the belly flop angle test. Feeding LS-DDG (14.1°) tended (P < 0.10) to create softer bellies compared with control-fed pigs. The PUFA content of belly fat was reduced (P < 0.01) by LS-DDG (14.0%) compared with DDGS (15.4%), but was increased (P < 0.05) compared with pigs fed the control (9.4%; pooled SEM = 0.34). In conclusion, LS-DDG and DDGS had similar ME values and inclusion of 20% LS-DDG in diets for growing-finishing pigs supports ADG and ADFI similar to that of diets containing 20% DDGS, and may reduce negative effects on pork fat compared with DDGS.     

Effects of feeding distillers dried grains with solubles, high-protein distillers dried grains, and corn germ to growing-finishing pigs on pig performance, carcass quality, and the palatability of pork

An experiment was conducted to investigate pig performance, carcass quality, and palatability of pork from pigs fed distillers dried grains with solubles (DDGS), high-protein distillers dried grains (HPDDG), and corn germ. Eighty-four pigs (initial BW, 22 +/- 1.7 kg) were allotted to 7 dietary treatments with 6 replicates per treatment and 2 pigs per pen. Diets were fed for 114 d in a 3-phase program. The control treatment was based on corn and soybean meal. Two treatments were formulated using 10 or 20% DDGS in each phase. Two additional treatments contained HP-DDG in amounts sufficient to substitute for either 50 or 100% of the soybean meal used in the control treatment. An additional 2 treatments contained 5 or 10% corn germ, which was calculated to provide the same amount of fat as 10 or 20% DDGS. Results showed that for the entire experiment, pig performance was not affected by DDGS or HP-DDG, but final BW increased (linear, P < 0.05) as corn germ was included in the diets. Carcass composition and muscle quality were not affected by DDGS, but LM area and LM depth decreased (linear, P < 0.05) as HP-DDG was added to the diets. Lean meat percentage increased and drip loss decreased as corn germ was included in the diets (quadratic, P < 0.05). There was no effect of DDGS on fat quality except that belly firmness decreased (linear, P < 0.05) as dietary DDGS concentration increased. Including HP-DDG or corn germ in the diets did not affect fat quality, except that the iodine value increased (linear, P < 0.05) in pigs fed HP-DDG diets and decreased (linear, P < 0.05) in pigs fed corn germ diets. Cooking loss, shear force, and bacon distortion score were not affected by the inclusion of DDGS, HP-DDG, or corn germ in the diets, and the overall palatability of the bacon and pork chops was not affected by dietary treatment. In conclusion, feeding 20% DDGS or high levels of HP-DDG to growing-finishing pigs did not negatively affect overall pig performance, carcass composition, muscle quality, or palatability but may decrease fat quality. Feeding up to 10% corn germ did not negatively affect pig performance, carcass composition, carcass quality, or pork palatability but increased final BW of the pigs and reduced the iodine value of belly fat.     

Effects of limit feeding corn or dried distillers grains with solubles at 2 intakes during the growing phase on the performance of feedlot cattle

Energy density in growing diets may affect carcass quality of cattle; however, few reports have described the impact of energy source. The objectives of this research were to determine effects of source [dried distillers grains with solubles (DDGS) vs. corn] and amount (limit-fed to gain 0.9 vs. 1.4 kg of BW/d) of energy during the growing phase on feedlot performance and marbling. Angus-cross steers (144 head) were blocked by BW (average initial BW = 252 ± 36 kg), allotted within each block to 8 pens (6 steers/pen, 24 pens total), and randomly assigned to 1 of 4 feeding systems in a 2 × 2 factorial arrangement of treatments: 1) 65% DDGS fed to gain 0.9 kg of BW/d, 2) 65% DDGS fed to gain 1.4 kg of BW/d, 3) 65% corn fed to gain 0.9 kg of BW/d, and 4) 65% corn fed to gain 1.4 kg of BW/d. Fecal grab samples were collected on d 52 of the growing phase to determine digestibility of DM, ADF, NDF, ether extract (EE), and CP. After the 98-d growing phase, all steers were fed the same finishing diet. Steers were slaughtered by pen when average BW within the pen was 544, 522, and 499 kg for the large, medium, and small BW blocks, respectively. Average daily gain and DMI differed (P<0.01) by design during the growing phase. Compared with the corn-based diets, digestibilities of DM, NDF, and EE were decreased (P<0.02) when DDGS-based diets were fed during the growing phase, whereas the digestibility of N was increased (P<0.01). The ADG was greatest (P=0.02) during the finishing phase for steers fed to gain 0.9 kg of BW/d initially, but source of energy during the growing phase did not affect (P=0.24) finishing phase ADG. Steers fed to gain 0.9 kg of BW/d during the growing phase also had less backfat (P=0.08), decreased USDA yield grades (P=0.03), and greater LM area (P<0.01) than steers fed to gain 1.4 kg of BW/d. There was an interaction between energy source and amount for marbling scores (P=0.02). Steers fed corn-based diets to gain 0.9 kg of BW/d during the growing phase had the most marbling, whereas those fed to gain 0.9 kg of BW/d on DDGS had the least marbling; the remaining feeding systems were intermediate. Overall ADG and DMI were affected (P < 0.06) by both source and amount of energy fed during the growing phase. Feeding the DDGS-based diet to achieve greater ADG during the growing phase increased marbling, whereas feeding the corn-based diet to increase ADG during the growing phase decreased marbling.     

Influence of postfast dietary crude protein and phosphorus content on nitrogen, phosphorus, calcium, and magnesium repletion in sheep.

An experiment was conducted to determine the influence of postfast dietary CP and P concentration on the repletion of N, P, Ca, and Mg lost during a 3-d fast in sheep. Four Suffolk wether lambs averaging 35 kg were used in a 4 x 4 Latin square design. Lambs were fed a control diet (700 g/d; as-fed basis) for 14 d and were then deprived of feed and water for 3 d. Lambs were then fed one of four isoenergetic realimentation diets: 1) low CP/low P, 2) low CP/high P, 3) high CP/high P, and 4) high CP/very high P. Realimentation N and Mg intakes were 9.8 and 1.1 g/d for lambs fed the low-CP diet and 18.1 and 1.7 g/d for lambs fed the high-CP diets, respectively. Realimentation P intakes were 1.40, 2.36, 2.66, and 3.82 g/d for lambs fed Diets 1, 2, 3, and 4, respectively. Nitrogen, P, Ca, and Mg apparent digestibility and balance and serum urea N, free fatty acids, P, Ca, Mg, and alkaline phosphatase were determined during the prefast, fast, and realimentation periods. Lambs fed the high-CP diets had higher (P less than .05) N and P digestibility and balance than lambs fed the low-CP diet. Increasing the dietary P content did not affect (P greater than .15) P balance or digestibility. In general, the realimentation diet fed did not affect (P greater than .15) serum concentrations of free fatty acids, alkaline phosphatase, inorganic P, Ca, or Mg.     

Winter-feeding systems for gestating sheep I. Effects on pre- and postpartum ewe performance and lamb progeny preweaning performance

Mature pregnant crossbred ewes (n = 90) were used in a randomized complete block design and assigned to 1 of 3 winter-feeding systems differing in primary feed source: haylage (HL), limit-fed corn (CN), or limit-fed dried distillers grains (DDGS). Effects of these winter-feeding strategies on ewe and lamb performance were determined. Diets were formulated to meet or exceed NRC (1985) nutrient requirements during gestation and were fed from about d 60 of gestation until parturition. All ewes were fed a common diet postpartum. Every 2 wk during gestation, BW and BCS were collected and diets were adjusted to maintain similar BW gain for ewes fed CN and DDGS vs. HL. At 80 and 122 d of gestation, jugular blood samples were collected at 0, 3, 6, and 9 h postfeeding to measure plasma glucose, insulin, NEFA, and blood urea nitrogen concentrations. At birth, 6 lambs per treatment were killed to measure body composition. At 28 ± 2 d postpartum, milk yield was measured. Lambs were weaned at 61 ± 4 d of age. During mid gestation (d 60 to 115), BW gain of ewes was similar among treatments; however, at d 115 of gestation ewes fed HL had a smaller (P = 0.04) BCS than ewes fed DDGS or CN. Plasma glucose concentrations were greater (P ≤ 0.004) in ewes fed CN than in those fed HL or DDGS just before feeding on d 80 and 122 of gestation, whereas ewes fed DDGS vs. CN or HL had greater (P ≤ 0.04) plasma insulin concentrations at 3 h postfeeding. At parturition, ewe BW was greatest for DDGS, least for HL, and intermediate for CN (P ≤ 0.003). Ewes fed CN and DDGS had greater BCS at parturition than those fed HL, but by weaning, ewes fed DDGS had greater BCS (P ≤ 0.05) than those fed CN or HL. Birth BW tended (P = 0.09) to be heavier for lambs from ewes fed CN and DDGS than from those fed HL prepartum, but there was no difference (P = 0.19) due to ewe gestation diet on lamb BW at weaning. At birth, lamb muscle, bone, organ, and fat measures were not affected (P > 0.13) by treatment. Ewe milk production and lamb preweaning ADG were also similar (P > 0.44) among treatments. Prepartum dam winter feed source did not have detrimental effects on pre- or postpartum ewe performance, but altered prepartum maternal nutrient supply during gestation, which affected birth weight but not preweaning growth or mortality.     

Terpenes and carbohydrate source influence rumen fermentation, digestibility, intake, and preference in sheep

We hypothesized that toxins and nutrients in foods interact to influence foraging behavior by herbivores. Based on this hypothesis we predicted that 1) terpenes in big sagebrush (Artemisia tridentata) influence intake and preference in sheep for diets varying in sources of nonstructural (barley grain) and structural (sugar beet pulp) carbohydrates, and 2) these effects are due to the differential effects of terpenes on fermentation products and apparent digestibility of each class of carbohydrates. Lambs were fed 2 isoenergetic and isonitrogenous diets with varying proportions of the same ingredients (beet pulp- and barley grain-based diet) or offered a choice between the 2 diets; all feeds were fed without and with terpenes, in consecutive periods. We also compared intake and preference of the beet pulp-and barley-based diets before and after the lambs ate a meal of sagebrush. Finally, we assessed the effect of terpenes on ruminal variables and in vivo digestibility. Lambs ate less when fed beet pulp or when they were offered a choice of diets with terpenes (P < 0.001), and intake of the beet pulp-based diet was the most affected (P < 0.05). Lambs preferred the beet pulp-to the grain-based diet with terpenes, but their preference reversed when terpenes were removed from the diets (P < 0.05). When lambs were offered both diets, intake and preference did not differ (P > 0.20) before eating sagebrush, but they preferred the beet pulp-based diet after eating sagebrush (P < 0.05). Intake of sagebrush did not differ among groups consuming the test diets (P = 0.21). Addition of terpenes to both diets increased the digestibility of DM, NDF, and ADF and decreased concentrations of total VFA and acetate (P < 0.05). Terpenes also depressed butyrate concentration in the barley-based diet (P < 0.05). Propionate concentrations were not affected by terpenes in either feed (P = 0.63). In summary, the predominant type of feed ingredient (beet pulp, grain) ingested with terpenes influenced fermentation products, intake, and preference in lambs. The source of energy from supplements, or other plants in the diet, is likely to influence intake and preference for sagebrush in sheep foraging on rangelands. Moreover, ingesting terpenes from sagebrush may also influence intake and preference for other plant species or supplements.     

Wheat distillers grains in feedlot cattle diets: feeding behavior, growth performance, carcass characteristics, and blood metabolites

A study was conducted to evaluate feed intake, ADG, carcass quality, eating behavior, and blood metabolites in feedlot beef steers fed diets that varied in proportion of wheat dried distillers grains with solubles (DDGS) replacing barley grain or barley silage. Two hundred crossbred steers (BW = 489 ± 30 kg) were blocked by BW and randomly allotted to 20 pens (5 pens per treatment). Steers were fed 1 of 4 diets: control without DDGS (CON), 25% (25DDGS), 30% (30DDGS), or 35% (35DDGS) wheat DDGS (DM basis). The CON diet consisted of 15% barley silage and 85% barley-based concentrate; the 3 wheat DDGS diets were formulated by substituting 20% barley grain and 5, 10, or 15% silage, respectively, with 25, 30, or 35% wheat DDGS so that the 35DDGS diet contained no silage. The diets were formulated such that wheat DDGS was substituted for both barley grain and barley silage to evaluate whether wheat DDGS can be fed as a source of both energy and fiber in feedlot finishing diets. Dry matter intake of steers fed 25DDGS was greater (P < 0.01), but final BW, ADG, and G:F were not different compared with steers fed CON diet. Carcass characteristics and liver abscess score were not different between CON and 25DDGS. Steers fed 25DDGS had longer eating time (min/d; P < 0.01), greater meal frequency (P < 0.04), but a slower eating rate (P < 0.04). Replacing barley silage with increasing amounts of wheat DDGS (from 25DDGS to 35DDGS) linearly reduced (P < 0.01) DMI. Final BW, ADG, and G:F were not affected by increasing amounts of wheat DDGS. Carcass traits were not different, whereas liver abscess scores linearly (P < 0.01) increased as more barley silage was replaced by wheat DDGS. Eating time (min/d) and duration of each meal linearly (P < 0.02) decreased, whereas eating rate (min/g of DM) linearly (P < 0.01) increased with increasing replacement of barley silage. Blood urea N was doubled (P < 0.01) compared with CON by inclusion of wheat DDGS. Results indicate that wheat DDGS can be used effectively in feedlot diets, decreasing the need for barley grain or silage without negatively affecting growth performance and carcass characteristics. A reduction in the amount of roughage required to maintain growth performance is a potential advantage in feedlot operations because forage is costly and often of limited availability. Thus, DDGS can be a possible alternative as long as they are available and cost effective; however, increased incidence of liver abscess and increased N content of manure need to be considered when greater amounts of wheat DDGS are included in finishing diets.     

Effect of grain processing degree on intake, digestion, ruminal fermentation, and performance characteristics of steers fed medium-concentrate growing diets

Three trials were conducted to evaluate the effects of degree of barley and corn processing on performance and digestion characteristics of steers fed growing diets. Trial 1 used 14 (328 +/- 43 kg initial BW) Holstein steers fitted with ruminal, duodenal, and ileal cannulas in a completely randomized design to evaluate intake, site of digestion, and ruminal fermentation. Treatments consisted of coarsely rolled barley (2,770 microm), moderately rolled barley (2,127 microm), and finely rolled barley (1,385 microm). Trial 2 used 141 crossbred beef steers (319 +/- 5.5 kg initial BW; 441 +/- 5.5 kg final BW) fed for 84 d in a 2 x 2 factorial arrangement to evaluate the effects of grain source (barley or corn) and extent of processing (coarse or fine) on steer performance. Trial 3 investigated four degrees of grain processing in barley-based growing diets and used 143 crossbred steers (277 +/- 19 kg initial BW; 396 +/- 19 kg final BW) fed for 93 d. Treatments were coarsely, moderately, and finely rolled barley and a mixture of coarsely and finely rolled barley to approximate moderately rolled barley. In Trial 1, total tract digestibilities of OM, CP, NDF, and ADF were not affected (P > or = 0.10) by barley processing; however, total tract starch digestibility increased linearly (P < 0.05), and fecal starch output decreased linearly (P < 0.05) with finer barley processing. In situ DM, CP, starch disappearance rate, starch soluble fraction, and extent of starch digestion increased linearly (P < 0.05) with finer processing. In Trial 2, final BW and ADG were not affected by degree of processing or type of grain (P > or = 0.13). Steers fed corn had greater DMI (P = 0.05) than those fed barley. In Trial 3, DMI decreased linearly with finer degree of processing (P = 0.003). Gain efficiency, apparent dietary NEm, and apparent dietary NEg increased (P < 0.001) with increased degree of processing. Finer processing of barley improved characteristics of starch digestion and feed efficiency, but finer processing of corn did not improve animal performance in medium-concentrate, growing diets.     

Sulfur concentration in diets containing corn, soybean meal, and distillers dried grains with solubles does not affect feed preference or growth performance of weanling or growing-finishing pigs

Four experiments were conducted to investigate the effects of distillers dried grains with solubles (DDGS) and dietary S on feed preference and performance of pigs. In a 10-d feed preference experiment (Exp. 1), 48 barrows (20.1 ± 2.2 kg of BW) were randomly allotted to 3 treatment groups, with 8 replicate pens per treatment and 2 pigs per pen. A control diet based on corn and soybean meal, a DDGS diet containing 20% DDGS, and a DDGS-sulfur (DDGS-S) diet were prepared. The DDGS-S diet was similar to the DDGS diet with the exception that 0.74% CaSO(4) was added to the diet. Two diets were provided in separate feeders in each pen: 1) the control diet and the DDGS diet, 2) the control diet and the DDGS-S diet, or 3) the DDGS diet and the DDGS-S diet. Preference for the DDGS diet and the DDGS-S diet vs. the control diet was 35.2 and 32.6%, respectively (P < 0.05), but there was no difference between the DDGS diet and the DDGS-S diet. In Exp. 2, a total of 90 barrows (10.3 ± 1.4 kg of BW) were allotted to 3 treatments, with 10 replicate pens and 3 pigs per pen, and were fed the diets used in Exp. 1 for 28 d, but only 1 diet was provided per pen. Pigs fed the control diet gained more BW (497 vs. 423 and 416 g/d; P < 0.05) and had greater G:F (0.540 vs. 0.471 and 0.455; P < 0.05) than pigs fed the DDGS or the DDGS-S diet, but no differences between the DDGS and the DDGS-S diets were observed. In a 10-d feed preference experiment (Exp. 3), 30 barrows (49.6 ± 2.3 kg of BW) were allotted to 3 treatment groups, with 10 replicates per group. The experimental procedures were the same as in Exp. 1, except that 30% DDGS was included in the DDGS and DDGS-S diets and 1.10% CaSO(4) was added to the DDGS-S diet. Feed preference for the DDGS and the DDGS-S diets, compared with the control diet, was 29.8 and 32.9%, respectively (P < 0.01), but there was no difference between the DDGS and the DDGS-S diets. In Exp. 4, a total of 120 barrows (34.2 ± 2.3 kg of BW) were fed grower diets for 42 d and finisher diets for 42 d. Diets were formulated as in Exp. 3. Pigs on the control diets gained more BW (1,021 vs. 912 and 907 g/d; P < 0.05) and had greater G:F (0.335 vs. 0.316 and 0.307; P < 0.05) than pigs fed the DDGS or DDGS-S diet, respectively, but no differences between pigs fed the DDGS and the DDGS-S diets were observed. In conclusion, dietary S concentration does not negatively affect feed preference, feed intake, or growth performance of weanling or growing-finishing pigs fed diets based on corn, soybean meal, and DDGS.     

Effects of dried distillers grains with solubles on growing and finishing pig performance in a commercial environment

Three experiments were conducted to determine the optimal level of dried distiller grains with solubles (DDGS) from a common ethanol manufacturing facility and to determine the potential interactions between dietary DDGS and added fat on performance and carcass characteristics of growing and finishing pigs. All experiments were conducted at the same commercial facility and used DDGS from the same ethanol manufacturing facility. In Exp. 1, a total of 1,050 pigs (average initial BW 47.6 kg), with 24 to 26 pigs per pen and 7 pens per treatment, were fed diets containing 0 or 15% DDGS and 0, 3, or 6% added choice white grease in a 2 x 3 factorial arrangement in a 28-d growth study. Overall, there were no DDGS x added fat interactions (P >/= 0.14). There was an improvement (linear, P < 0.01) in ADG and G:F as the percentage of added fat increased. There was no difference (P = 0.74) in growth performance between pigs fed 0 or 15% DDGS. In Exp. 2, a total of 1,038 pigs (average initial BW 46.3 kg), with 24 to 26 pigs per pen and 10 pens per treatment, were fed diets containing 0, 10, 20, or 30% DDGS in a 56-d growth study. Pigs fed diets containing DDGS had a tendency for decreased ADG and ADFI (both linear, P = 0.09 and 0.05, respectively), but the greatest reduction seemed to occur between pigs fed 10 and 20% DDGS. In Exp. 3, a total of 1,112 pigs (average initial BW 49.7 kg), with 25 to 28 pigs per pen and 9 pens per treatment, were used in a 78-d growth study to evaluate the effects of increasing DDGS (0, 5, 10, 15, or 20%) in the diet on pig growth performance and carcass characteristics. From d 0 to 78, ADG and ADFI decreased linearly (P 相似文献   

Utilization of distillers dried grains with solubles and phytase in sow lactation diets to meet the phosphorus requirement of the sow and reduce fecal phosphorus concentration

Two experiments were completed to determine the potential for using distillers dried grains with solubles (DDGS) in diets with or without phytase to provide available P, energy, and protein to highly productive lactating sows without increasing their fecal P. In Exp. 1, the dietary treatments were as follows: (1) corn and soybean meal with 5% beet pulp (BP) or (2) corn and soybean meal with 15% DDGS (DDGS). Besides containing similar amounts of fiber, diets were isonitrogenous (21% CP, 1.2% Lys) and isophosphorus (0.8% P). Sixty-one sows were allotted to dietary treatments at approximately 110 d of gestation (when they were placed in farrowing crates) based on genetics, parity, and date of farrowing. Sows were gradually transitioned to their lactation diet. On d 2 of lactation, litters were cross-fostered to achieve 11 pigs/litter. Sows and litters were weighed on d 2 and 18. Fecal grab samples were collected on d 7, 14, and 18 of lactation. Dietary treatment did not affect the number of pigs weaned (10.9 vs. 10.8) or litter weaning weight. On d 14, DDGS sows had less fecal P concentration than BP sows (28.3 vs. 32.8 mg/g; P = 0.04). Fecal Ca of sows fed DDGS decreased for d 7, 14, and 18 (55.6, 51.4, and 47.1 mg/g of DM, respectively; P = 0.05) but not for BP sows. In Exp. 2, the dietary treatments were as follows: (1) corn and soybean meal (CON), (2) CON + 500 phytase units of Natuphos/kg diet, as fed (CON + PHY), (3) corn and soybean meal with 15% DDGS and no phytase (DDGS), or (4) DDGS + 500 FTU of Natuphos/kg of diet, as fed (DDGS + PHY). Sows (n = 87) were managed as described for Exp 1. Litter BW gain (46.0, 46.3, 42.1, and 42.2 kg; P = 0.25) and sow BW loss (8.1, 7.2, 7.4, and 6.3 kg for CON, CON + PHY, DDGS, and DDGS + PHY, respectively; P = 0.97) were not affected by dietary treatment. Fecal P concentration did not differ among dietary treatments but was reduced at d 14 and 18 compared with d 7 (P = 0.001). However, fecal phytate P concentration was decreased by the addition of DDGS when DDGS and DDGS + PHY were compared with the CON sows except on d 7 (P < 0.05). Sows fed CON diet had greater fecal phytate P than sows fed DDGS, and sows fed DDGS + PHY had less fecal phytate P than sows fed DDGS with no phytase (P = 0.001). Although these experiments were only carried out for 1 lactation, these results indicate that highly productive sows can sustain lactation performance with reduced fecal phytate P when fed DDGS and phytase in lactation diets.