Changes in nutritive value and herbage yield during extended growth intervals in grass–legume mixtures: effects of species,maturity at harvest,and relationships between productivity and components of feed quality

There is a lack of information on the effects of companion species in grass–legume mixtures on herbage yield and quality changes during prolonged growth. Such information is relevant for harvest planning and estimation of consequences for feeding value of conserved feed when harvesting is delayed. Perennial ryegrass was sown with each of four legumes: red clover, white clover, lucerne and birdsfoot trefoil, and white clover was sown with hybrid ryegrass, meadow fescue and timothy. Effects of species composition on herbage yield, contents of N, neutral detergent fibre (NDF), acid detergent fibre (ADF) and lignin, ash and in vitro organic matter digestibility (IVOMD) were studied in entire herbage and in component species during 2 years in a small‐plot cutting trial in Denmark. In May and August, the dynamic development of components of feed value and their interrelationships were investigated by sampling at optimum harvest date (i.e., normal practice) ± 1 week (t = ?1 to t = +1). Herbage digestibility and contents of N and ash decreased while those of fibre compounds increased during the 2 weeks from t = ?1 to t = 1 in all species. In May, contents of ADF and lignin increased at a faster rate in legumes than in grasses; in August, NDF and ADF increased most quickly in legumes. Generally, N contents and IVOMD declined at similar rates in grasses and legumes, but, within each group, differences in yield and quality development occurred among species. The relationship between weekly growth rate and change in quality parameters differed among species and functional groups, i.e., grasses and legumes. Results are discussed in the context of quantifying the impact of delaying the harvest date of grass–legume mixtures and relationships between productivity and components of feed quality.     

Seeding cool-season grasses into unimproved warm-season pasture in the southern Great Plains of the United States

Limited availability of herbage during the cool season creates a problem of a supply of nutrients for livestock producers throughout the southern Great Plains of the USA and, particularly, on small farms where resource constraints limit possible mitigating strategies. Six cool‐season grasses were individually sown into clean‐tilled ground, no‐till drilled into stubble of Korean lespedeza [Kummerowia stipulacea (Maxim) Makino] or no‐till over‐sown into dormant unimproved warm‐season pastures. The dry matter (DM) yields of mixtures of cool and warm‐season herbage species were measured to test their potential for increasing cool‐season herbage production in a low‐input pasture environment. Only mixtures containing Italian ryegrass (Lolium multiflorum Lam) produced greater year‐round DM yields than undisturbed warm‐season pasture with all establishment methods. When cool‐season grass was no‐till seeded into existing warm‐season pasture, there was on average a 0·61 kg DM increase in year‐round herbage production for each 1·0 kg DM of cool‐season grass herbage produced. Sowing into stubble of Korean lespedeza, or into clean‐tilled ground, required 700 or 1400 kg DM ha^?1, respectively, of cool‐season production before the year‐round DM yield of each species equalled that of undisturbed warm‐season pasture. Productive pastures of perennial cool‐season grasses were not sustained beyond two growing seasons with tall wheatgrass [Elytrigia elongata (Host) Nevski], intermediate wheatgrass [Elytrigia intermedia (Host) Nevski] and a creeping wheatgrass (Elytrigia repens L.) × bluebunch wheatgrass [Pseudoroegneria spicata (Pursh)] hybrid. Lack of persistence and low productivity limit the usefulness of cool‐season perennial grasses for over‐seeding unimproved warm‐season pasture in the southern Great Plains.     

Effects of shrub encroachment on herbage production and nutritive value in semi-arid Mediterranean grasslands

There is limited information on the effects of the increase in the density of shrubs on herbage production and nutritive value of natural grasslands in the Mediterranean region, currently facing major land use changes. Herbage production of drymatter (herbaceous fractions, of plant functional groups and by species), crude protein (CP), neutral‐detergent fibre (NDF), acid‐detergent fibre (ADF), acid‐detergent lignin (ADL) and hemicellulose concentrations and in vitro organic matter digestibility were determined at the time of peak of annual growth across four types of grassland vegetation each characterized by different shrub cover regimes. A sharp reduction in herbage production and a reduction in nutritive value were found as a result of the increase in shrub cover. These changes appeared to be closely related to the shift in plant functional groups detected as shrub density increased. Herbage production from grasses and legumes was found to be more sensitive to shrub cover changes than herbage production from forbs, whereas, as grassland types became denser, annual species were gradually replaced by perennials and C₄ grasses by C₃ ones. The impact of shrub encroachment on Mediterranean grasslands is discussed in relation to their use by livestock.     

THE EFFECT OF COMPANION GRASS AND SEED RATE ON THE PRODUCTIVITY OF A TALL-FESCUE SWARD

The effects of sowing from 10 to 50 lb/acre of tall-fescue seed on plant establishment and on the annual and seasonal distribution of DM yield of tall fescue and its companion grasses were measured through two growing seasons. Increasing the seed rate increased plant numbers per unit area and decreased per cent establishment. Adding Scots timothy as a companion grass depressed both plant number and per cent establishment. S37 cocksfoot and New Zealand perennial ryegrass reduced both still further. Total annual dry-matter production was relatively unaffected by varying either the seed rate or the companion grass. The contribution of tall fescue to total production was highest when sown alone and was successively reduced by timothy, perennial ryegrass and cocksfoot. When sown alone there were only slight differences in contribution of tall fescue due to seed rate. With any companion grass the contribution from tall fescue increased with increasing seed rate. Early growth was not reduced by modifying seed rate but was reduced by all the companion grasses. It is concluded that increasing the seed rate of tall fescue above 30 lb is not justified, that Scots timothy can be sown at 3 lb/acre with tall fescue without affecting total production or early growth of the mixture, with the advantages of control of unsown species and improved palatability of the herbage.     

Effect of sowing date of triticale on seasonal herbage production in the central Appalachian Highlands of the United States

Triticale (X Triticosecale Wittmack) was evaluated as a complementary pasture to buffer those periods when herbage production from mixed perennial pasture is marginal in the central Appalachian Highlands of the United States. Triticale was sown every month from May to October for five consecutive years from 1999 to 2003. Plant population structure and herbage production were evaluated at intervals until May of the year following establishment. Triticale established quickly at all times of sowing except late October. Wet summers resulted in foliar disease and a rapid decline in plant density when triticale was sown in May and June. In contrast, during the relatively dry summer of 1999, triticale stands exhibited minimal decline. Triticale sown in August had a herbage yield of 1580 kg DM ha^?1 when harvested in October which was over twice the herbage yield of triticale sown in May, June and July. Average herbage yield in the following April of triticale sown in September was higher (1750 kg DM ha^?1) and less variable than herbage yields from other sowing dates. Plant and tiller populations declined throughout the following April but herbage yields in May were high due to stem and seed head development associated with reproductive growth. Incorporating areas of triticale into mixed‐species perennial pasture systems could buffer herbage production during hot and dry summer periods as well as during cool periods of late autumn and early spring.     

Forage herbs improve mineral composition of grassland herbage

Provision of an adequate mineral supply in the diets of ruminants fed mainly on grassland herbage can present a challenge if mineral concentrations are suboptimal for animal nutrition. Forage herbs may be included in grassland seed mixtures to improve herbage mineral content, although there is limited information about mineral concentrations in forage herbs. To determine whether herbs have greater macro‐ and micromineral concentrations than forage legumes and grasses, we conducted a 2‐year experiment on a loamy‐sand site in Denmark sown with a multi‐species mixture comprised of three functional groups (grasses, legumes and herbs). Herb species included chicory (Cichorium intybus L.), plantain (Plantago lanceolata L.), caraway (Carum carvi L.) and salad burnet (Sanguisorba minor L.). We also investigated the effect of slurry application on the macro‐ and micromineral concentration of grasses, legumes and herbs. In general, herbs had greater concentrations of the macrominerals P, Mg, K and S and the microminerals Zn and B than grasses and legumes. Slurry application indirectly decreased Ca, S, Cu and B concentrations of total herbage because of an increase in the proportion of mineral‐poor grasses. Our study indicates that including herbs in forage mixtures is an effective way of increasing mineral concentrations in herbage.     

The long-term effects of a range of pasture treatments applied to three semi-natural hill grassland communities. 1. Pasture production and botanical composition

Five pasture treatments: (1) controlled grazing, (2) controlled grazing + lime, (3) controlled grazing + lime + phosphate, (4) controlled grazing + lime + phosphate + oversown white clover and (5) controlled grazing + lime + phosphate + oversown white clover + oversown perennial ryegrass were applied to three semi-natural hill grassland communities. The communities were those dominated by Agrostis/Festuca, Molinia and Nardus. The proportion of Nardus at the Nardus-dominant site was substantially reduced by herbicide before treatments were applied. All treatments were grazed simultaneously by mature wether sheep on three occasions each year. There were two grazing periods each of 4 weeks duration between mid-May and mid-August with a further grazing period of 3 weeks starting mid-October. During each grazing period stock numbers were set so that a residual herbage mass of 560 kg DM ha ^?1 remained at the end of the grazing period. Measurements of net herbage accumulation (NHA) were made annually over a period of 13 years at each site. The green:dead ratio of grasses, species composition of the pasture and the pH of the soil were measured at intervals during the experiment. Estimates of mean annual NHA ranged from 3860 kg DM ha^?1 for treatment 1 (controlled grazing) to 5170 kg DM ha^?1 for treatment 5 (oversown white clover and perennial ryegrass). The application of lime and phosphate increased annual NHA by 300–350 kg DM ha^?1 with a further increase of around 400 kg DM ha^?1 when white clover was sown. Increases in NHA between year 1 and year 13 ranged from 30% for treatment 1 to around 55% for treatment 5. Although there was no difference in the mean NHA between sites, the herbage from the Agrostis/Festuca site contained a higher proportion of green grass and white clover than that from the other sites. The highest levels of green grass and white clover were found on the oversown treatments at each site. The grazing pressure exerted produced relatively little change in the botanical composition at the Agrostis/Festuca site. At the Molinia-dominant site the Molinia was largely replaced by Nardus during the first 6 years and Nardus also increased in cover at the Nardus site. Application of lime and phosphate generally increased the proportion of Poa pratensis, Festuca rubra and Agrotis tenuis but did not halt the spread of Nardus at the Molinia and Nardus sites. White clover and perennial ryegrass were successfully introduced by oversowing and proportions remained high throughout the 13 years. The cover by bryophyte spp. increased at all sites with the greatest increases occurring in each case on the less comprehensive pasture treatments. These results suggest that on Agrostis/Festuca-dominant pastures herbage biomass production can be increased with relatively low-cost pasture treatments while maintaining Species diversity. However, Nardus and Molinia dominant pastures are likely to require more comprehensive pasture treatments involving sown grasses and white clover to provide herbage of acceptable quality and avoid an increase in Nardus and bryophytes in the sward. With a regime of episodic summer grazing and the addition of fertilizers oversown pastures can be maintained over long periods of time.     

PASTURE DEVELOPMENT IN THE BEEF CATTLE REGIONS OF ARGENTINA

An account is given of the climate, vegetation and native pastures of the beef-cattle regions of Argentina. Though some of these native pastures have a satisfactory carrying capacity, most of them are defective in quality or low in productivity; there is considerable scope for increasing production through the establishment of sown pastures. Trials have been made with a wide range of grasses and legumes, principally of African and European origin. Though the pattern of species to be used in these regions is imperfectly resolved, the authors present a map showing tentative boundaries for temperate, sub-tropical and tropical species. Work to date suggests that pasture development can probably proceed over great areas without the use of fertilizers, and this adds to the attractiveness of pasture programmes. A problem of no less importance than the technical aspects of pasture improvement is that of gaining a greater investment of capital into the development of cattle estancias.     

Main ecological and environmental factors affecting forage yield and quality in alpine summer pastures (NW-Italy,Gran Paradiso National Park)

Summer pastures in the European Alps play a crucial role in providing high quality forage for grazing livestock and encompass a wide variety of vegetation communities. The main issue of their management is the optimal exploitation of the available forage in relation to environmental constraints, aiming at obtaining the highest forage yield and quality from each vegetation community. In this work, we monitored six different vegetation communities characterized by contrasting topographic features and species compositions throughout the grazing season for two years. We performed botanical and phenological surveys, climate-related measurements, and herbage samplings to assess forage quantity and quality (i.e., digestibility and proximate composition, estimated by near-infrared reflectance spectroscopy). We analysed the influence of climatic, topographic, and vegetation variables on pasture herbage through univariate and multivariate analyses. The cover of wide-leaf grasses primarily promoted biomass production, which was in turn lowered by increasing elevation and slope. On the other hand, a minor effect on forage yield was observed for an increasing cover of sedges and rushes and mean temperatures. Climatic variables (especially water deficit) and advanced phenological stages were the main degrading factors of forage quality in terms of crude protein and digestibility, while enhancing fibre contents. At increasing cover of legumes, fibre content declined and digestibility increased consequently, while the abundance of other non-legume forbs played a significant role in lowering fibre content. Multivariate analysis highlighted the differing influence of single plant species on forage features even within the same functional species pool. However, attention should be paid to the toxicity and low palatability of some plants, especially forbs. Eutrophic vegetation communities, rich in wide-leaf grasses and legumes, could be maintained and enhanced through pastoral management to increase alpine pasture production and quality.     

The assessment of persistence and its application to the evaluation of mid-season and late perennial ryegrass cultivars

Persistence assessments on eight mid-season and ten late cultivars of perennial ryegrass were made at the end of the second, third and fourth years after sowing. Botanical analyses were carried out on the herbage from plots cut in the fourth year and these results related lo the different persistence assessments. It was found that persistence assessments carried out at the end of the second year enabled predictions to be made about botanical composition of cuitivars in the fourth year. Little extra precision was gained by delaying assessment until the third or fourth years as the ranking order of cultivars with respect to persistence remained largely unchanged. All persistence assessments were positively correlated with yields of sown cultivars and negatively correlated with yields of unsown herbage species in the fourth year. However, only low correlation coefficients were obtained between persistence assessments and the total yield of sown cultivar and unsown species in the fourth year, due to yield substitution between these two components. In swards sown as monocultures low persistence in a cultivar is considered to be a lack of perenniality and tiller regeneration rather than poor competitive ability against volunteer species though in mixed swards competitive ability per se will have considerable influence upon the changes in botanical composition under different managements. Persistence and yield capacity are seen, however, as distinct characteristics of a cuitivar and long-term yield potential is a joint function of these characteristics.     

Impact of deferred grazing and fertilizer on herbage production,soil seed reserve and nutritive value of native pastures in steep hill country of southern Australia

Developing sustainable grazing management systems based on perennial species is critical to preventing land degradation in marginal land classes. A field study was conducted from 2002 to 2006 to identify the impacts of deferred grazing (no defoliation of pastures for a period generally from spring to autumn) and fertilizer application on herbage accumulation, soil seed reserve and nutritive value in a hill pasture in western Victoria, Australia. Three deferred grazing strategies were used: short‐term deferred grazing (no defoliation between October and January), long‐term deferred grazing (no defoliation from October to the autumn break) and optimized deferred grazing (withholding time from grazing commenced between annual grass stem elongation and seed head emergence and concluded in February/March). These treatments were applied with two fertilizer levels (with or without fertilizer at 50 kg phosphorus ha^?1 and 2000 kg lime ha^?1 applied in year 1 only) in a factorial arrangement and two additional treatments: continuous grazing (CG) and no grazing (NG) in year 1. The deferred grazing treatments on average produced herbage dry matter of 4773 kg ha^?1, the NG produced 4583 kg ha^?1 and the CG produced 3183 kg ha^?1 in year 4 (2005–06) of the experiment. Deferred grazing treatments with and without fertilizer application produced an average of 5135 and 4411 kg DM ha^?1 respectively. Averaged over 4 years, deferred grazing increased the germinable seed pool of perennial grasses by 200% and annual grasses by 50% (except optimized deferred grazing that considerably decreased the annual grass seed pool) compared with the CG. The best of the deferred grazing strategies increased the digestibility of pastures by 7% compared with the CG. The results demonstrated that deferred grazing from spring to autumn followed by rotational grazing could be an effective tool to increase herbage production and soil seed pool and improve the digestibility of native pastures in the steep hill country of southern Australia.     

Herbage production from swards containing a range of grass, forb and clover species and under extensive management

Three small plot experiments were conducted to investigate the effects of species of grass and forbs, defoliation regime, inclusion of white clover and forb blend on the herbage dry matter (DM) yield, botanical composition and mineral content of swards managed with zero fertilizer inputs. The results of all three experiments were characterized by decline in herbage production and large variations in treatment effects over the harvest period.
When sown singly with a standard grass mix the species that competed well with grasses and produced annual forb herbage yields greater than 20 t DM ha⁻¹ were black knapweed, oxeye daisy, ribwort plantain, burnet, birdsfoot trefoil, chicory, kidney vetch, red clover and white clover. When sown singly with a standard forb mix, grass species significantly affected the annual yield of total ( P <005). grass ( P <001) and forb ( P <0.001) herbage. The species that most surpressed the yield of forbs were common bent, Yorkshire fog and perennial ryegrass. Those that allowed for the highest yield of forbs were rough meadow grass, sweet vernal grass and crested dogstail. Averaged over the three harvest years, defoliation regime did not significantly affect herbage production, but the inclusion of white clover in mixtures increased the yield of grasses ( P <0.01) The use of rosette-type forb blends increased forb yield ( P <0.01), compared with erect-type blends.
The effects of treatments on herbage N and mineral contents and yields were inconsistent. However, there was some evidence to support the view that the presence of forb species in swards can result in greater contents of minerals in herbage, compared with grass-only swards.     

The utilization of sown and indigenous plant species by sheep and goats grazing hill pastures

The utilization of sown and indigenous plant species was studied in three experiments in which plots were stocked with similar live weights of sheep and goats. In the first experiment the animals grazed plots containing 0–5 ha of rush ( Juncus effusus )- infested reseeded pasture and 0–5 ha of unimproved blanket bog. The second and third experiments took place on old rush-infested improved pasture; in one experiment two levels of herbage mass of grass were provided while in the other the rushes were cut in spring or remained uncut.
The goats grazed the rushes readily in all three experiments. Reduction in herbage mass of grass increased utilization of rushes by goats although these animals still grazed rushes when grass supply was plentiful. Sheep scarcely grazed J. effusus even when hard-pressed by shortage of grass. Both sheep and goats grazed J. acutiflorus.
In Experiment I sheep utilized reseeded pasture more heavily than did goats. Sheep grazed similar proportions of the leaves of grass and clover while goats grazed a lower proportion of clover leaves as compared with grass. Among the grasses sheep discriminated in favour of Lolium perenne whereas goats did not. On the blanket bog vegetation, Eriophorum vaginatum and Calluna vulgaris were grazed both earlier in the season and more heavily by goats than by sheep.
The relationships between sward structure and grazing height in accounting for differences in species selection by sheep and goats are briefly discussed. The possibility of using goals to control coarse weeds in hill pasture and for strategic grazing to manipulate floristic composition is outlined.     

Grazing preference of Merino sheep for naturalized annual clover species relative to commonly sown clover species

Selective grazing of white clover (Trifolium repens) over grass species in temperate pastures results in reduced clover abundance and availability over time. Within sheep‐ and cattle‐grazed dryland (<800 mm annual rainfall) hill and high country areas of New Zealand, naturalized unsown annual clover species show greater persistence and abundance over sown clovers. With a view to understanding legume abundance in these areas, Merino sheep grazing preference was investigated for pure swards of naturalized species Trifolium dubium, T. glomeratum, T. arvense and T. striatum and commonly sown species T. repens and T. subterraneum. The Chesson–Manly preference index was used to explore the hypothesis that grazing preference differs between these species and changes as plants mature. Herbage offtake was quantified at vegetative (mid–late spring; November) and reproductive (early summer; December) stages of plant maturity. Significant preference distinctions between species (P < 0·05) occurred in December, with relative preference ranging from 0·248 for T. repens to 0·065 for T. dubium. Reduced relative preference for naturalized species was related to decreasing nutritive value from spring to summer, reflecting increased stem and flower sward content. Relative preference was negatively related to increasing acid detergent fibre and neutral detergent fibre and positively related to greater crude protein and dry‐matter digestibility. Naturalized annual clover species of lower grazing preference at reproductive maturity will benefit pasture sustainability via herbage and seed production and associated nitrogen‐fixation inputs.     

Seasonal productivity and nutritive value of temperate grasses found in semi-natural pastures in Europe: responses to cutting frequency and N supply

Monocultures of thirteen perennial C₃ grass species that co‐occur in temperate semi‐natural grassland communities in Europe were compared in a factorial field design of two levels of N supply and two levels of cutting frequency. Above‐ground yield of dry matter (DM), crude protein (CP) concentration and pepsin‐cellulase DM digestibility of herbage were measured in two successive years. Species was the largest source of variability in yield of DM and DM digestibility of herbage, while CP concentration of herbage responded more to management factors. The highest mean DM yields and values of DM digestibility of herbage were achieved in spring for Festuca arundinacea and in autumn for Phleum pratense. Poa trivialis and Festuca rubra had the lowest DM yield and DM digestibility values, respectively, regardless of seasons and treatments. For all species CP concentration in herbage increased in response to an increase in cutting frequency and N supply by an average of 46 and 34 g kg^?1 DM respectively. Differences between years and seasons indicated the importance of plant phenology on nutritional variables and the influence of environmental factors on species performance. Species ranking was compared according to their annual digestible DM and CP yields. The results show that some grasses have a nutritive value which is comparable to that of forages selected for high yields.     

Organic and inorganic fertilization compared in a natural Mediterranean grassland

The effect of goat slurry on species composition and herbage production as compared to inorganic fertilizer was studied in a natural grassland dominated by warm and cool season perennial grasses in Macedonia, Greece. Goat slurry was applied in early spring every 1 or 2 years at a rate of 40 t ha^-1 being equivalent to about 160 kg N ha^-1 and 100 kg, P₂O₅ ha^-1. Inorganic fertilizer was also broadcast in early spring at a rate of 80 kg N ha^-1 and 100 kg P₂O₅ ha^-1 every 2 years. Measurements of the basal cover of the dominant species or groups of species and herbage yields were taken for 6 years at the end of the growing period in June, while in the seventh year the experiment was grazed with goats. It was found that goat slurry improved species composition more than the inorganic fertilizer by depressing the less palatable warm season grasses and favouring the subdominant cool season grasses and legumes. Goat slurry significantly increased herbage yields in most of the years. It is suggested that the optimum rate of goat slurry is 40 t ha^-1 every 2 years as this encourages plants most preferred by goats.     

Changes in the population of germinated seed in a hill pasture during and after a pastoral fallow

A pastoral fallow, which involves no defoliation of pasture for a period, generally from spring to autumn, is a management tool that can be used either to reduce plant population density through self-thinning prior to oversowing improved plant germplasm or to encourage natural reseeding in order to increase sward persistence and productivity. Two field experiments were carried out in a hill pasture in New Zealand to quantify the response of the seed population, germinated from soil plugs under glasshouse conditions for over 7 months, to pastoral fallow, as influenced by the timing and duration of the fallow, fertilizer application and aspect, and to identify the germination patterns of different plant species in fallowed and grazed swards. A spring–autumn pastoral fallow increased the germinated seed population of grasses, legumes and weeds by 161%, 51% and 59%, respectively, compared with the grazed control. The variation in germinated seed population during the pastoral fallow showed that seed quantity can be controlled by manipulating the timing and duration of a pastoral fallow. Short duration (2–3 month) pastoral fallows resulted in a significantly lower germinated grass seed population than the full (spring–autumn) fallows. Fertilizer application reduced the germinated grass seed population by 26%. The germinated legume seed population was higher with a shady than a sunny aspect. Regression analysis revealed that the germination patterns of all plant species followed a negative exponential function. Partial differentiation of this function derived a germination rate curve on which a two-pool (ephemeral pool and base pool) model was developed to describe quantitatively the dynamics of germinable seed reserves in the soil.     

More lightweight and isodiametric seeds for C4 than for C3 grasses are associated with preference for open habitats of C4 grasses in a temperate flora

Seed mass and shape of grasses were analysed in a temperate flora containing 178 and 26 species belonging to C₃ and C₄ photosynthetic types respectively. The weedy character and the annual or perennial status were also considered. On the basis of the seed traits studied, three groups were distinguished: C₄ grasses, annual C₃ grasses of weedy character and perennial non‐weedy C₃ grasses. The C₄ group had more isodiametric (same diameter in all directions) seed shape and lower average seed mass than the C₃ group. To our knowledge, this has not yet been described for temperate C₄ grasses and is certainly associated with their preference for open habitats where competition for light is small. Weedy annual C₃ grasses had heavier and less isodiametric seeds than C₄ grasses did. These species are mostly specialized to establish in the dense cover of perennial vegetation, and this ability distinguishes them from the C₄ group. Non‐weedy perennial C₃ species possessed less isodiametric seeds than did C₄ grasses, but did not differ from weedy annual C₃ grasses. As most alien C₄ grasses naturalized in Hungary are annuals with small, isodiametric seeds, these traits are good candidates to be included in screening for potential future invasives in open habitats.     

The assessment of persistence and its application to the evaluation of early perennial ryegrass cultivars

Botanical analyses were carried out on the herbage from plots of twelve early cultivars of perennial ryegrass (Lolium perenne) cut in their fourth year and the results related to visual persistence assessments carried out on the cultivars at the end of the second and third harvest years. Persistence assessments were found to be highly and positively correlated with the yields of sown cultivars (r=+0.96***,r=+0.99***) and to be highly and negatively correlated with the yields of unsown herbage species (r =+0.98***, r =?094***). The plots of the less persistent cultivars were invaded by unsown grass species and docks which contributed in varying degrees to total DM yield, reducing the correlation of total yield with persistence. Assessments of botanical composition of swards made at the end of the second year provided reliable predictions of the relative persistence of the cultivars. The results of the investigations indicated that assessment of persistence, as well as yield potential, is fundamentally important in cultivar evaluation.     

Spring and autumn establishment of Caucasian and white clovers with different sowing rates of perennial ryegrass

The effect of sowing date (SD) and sowing rate of perennial ryegrass (PRG) on the establishment of Caucasian and white clovers in New Zealand was assessed. Clovers were sown in spring on 24 September (SD1) and 9 November (SD2) 1999, and in autumn on 4 February (SD3) and 31 March (SD4) 2000. On each date, clovers were sown with 0, 3, 6 or 12 kg ha^?1 of PRG. Total herbage dry matter (DM) production up to 6 November 2000 was 13–16 t DM ha^?1 for SD1 and SD2 when sown with 3–12 kg ha^?1 of PRG, and 7–10 t DM ha^?1 for sown clover monocultures. For SD3 and SD4, total herbage production was 6–9 t DM ha^?1 with PRG, while total herbage production of clover monocultures was 5·4 t DM ha^?1 for SD3 and 2·6 t DM ha^?1 for SD4. By 6 November 2000, white clover contributed proportionately more than 0·15 of herbage mass when sown with 3–12 kg ha^?1 of PRG on SD1, SD2 or SD3, but less than 0·09 when sown on SD4. The proportion of Caucasian clover never exceeded 0·09 of herbage mass in any of the swards. White clover was successfully established in spring and in autumn with 3–12 kg ha^?1 of PRG provided the 15‐mm soil temperature was above 14 °C. None of the combinations of Caucasian clover and PRG provided an adequate proportion of legumes during the establishment year. This unsuccessful establishment of Caucasian clover with PRG was attributed to its inability to compete for available light as a seedling due to slow leaf area expansion from secondary shoot development and a high root:shoot ratio. Alternative establishment strategies for Caucasian clover may include the use of slow establishing grasses, cover crops and temporal species separation.