Proteomic analysis of colon cancer

AIM: To investigate the cancer associated proteins and sensitive biomarkers in colon cancer. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expression in cancer and matched morphologically normal colonic mucosa from 8 patients. Proteins that showed differential expression of a 2-fold change were cut and analyzed by MALDI-TOF mass spectrometry. RESULTS: Two-dimensional protein maps of cancer and matched normal tissue were gained successfully. Gel-analysis software identified an average of 3289 spots in cancer while 3066 in matched normal tissue and statistical filtering yielded 31 spots of a 2-fold change, 18 of which were identified by using mass spectrometry, including keratin 8, S100A6, protein disulfide isomerase, etc. Functional analysis revealed that these proteins were associated with cancer cellular oncogenesis, proliferation, differentiation and metastasis. CONCLUSION: Proteomic analysis can identify the proteins with variance of colon cancer versus normal colonic tissue as well as providing probable new biomarkers correlated with biological behavior of colon cancer.     

Proteomic analysis between pathological scars and normal skin

AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars.     

Identification of serum differential proteins in colorectal adenoma and early malignantly transformed adenoma by proteomics

AIM: To investigate the associated proteins and sensitive biomarkers for early diagnosis of colorectal adenocarcinoma by comparing the results of differential proteomic analysis between colorectal adenoma and early malignantly transformed adenoma. METHODS: Two-dimensional gel electrophoresis was used to define patterns of protein expressions of colorectal adenoma and early malignantly transformed adenoma. Proteins expressed differentially among groups were detected, cut out and analyzed by MALDI-TOF/TOF mass spectrometry. RESULTS: Two-dimensional protein maps of colorectal adenoma and early malignantly transformed adenoma were analyzed with gel-analysis software, an average of 1 672 spots in adenoma, 1 732 in early malignantly transformed adenoma were observed. 28 spots of a 1.5-fold change were found, including 15 proteins down-regulated and 13 up-regulated in early malignantly transformed adenoma, in which 23 proteins were identified by mass spectrometry, the rate of identification was 82.14%. 13 differential proteins were attained, 8 were up-regulated and 5 were down-regulated, which was classified to 6 categories, including protease inhibitor, complement, immunoglobulin, keratoproteins, signal transduction protein and function-unknown proteins. CONCLUSION: The changes of serum proteins in early malignantly transformed adenoma from adenoma can be identified by proteomic technology. Proteins detected in the study may provide new biomarkers correlated with biological behavior of colorectal adenocarcinoma.     

Proteomics analysis of sera from Crohns disease patients and healthy individuals

AIM: To explore differentially expressed proteins of sera from Crohn's disease patients with comparative proteomics technology.METHODS: Two-dimensional differential in-gel electrophoresis was used to define patterns of protein expression in serum cross-labeled with variant CyDye from 4 patients and matched health adults.Proteins that showed differential expressions were analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry.RESULTS: Two-dimensional cross-labeled with variant CyDye protein maps of Crohn's disease patients and the health adults were gained successfully.Gel-analysis software identified differently expressed 29 spots Crohn's disease compared with that of health adults,22 of which were identified by using mass spectrometry,including serine/threonine-protein kinase ATR,leukocyte common antigen precursor CD45,60 kD SS-A/Ro ribonucleo-protein,etc.CONCLUSION: Proteomic analysis can identify the serum proteins with variance of Crohn's disease versus health,the different expressed proteins may providing probable new biomarkers correlated with biological behavior of Crohn's disease.     

Differential proteomic analysis of liver in apoE/LDLR double-gene mutant mice

AIM: To comparatively study the differential expression of protein profiles of liver between double lipid metabolism genes mutant (apoE-/-/LDLR-/-) and wild type（WT）mice.The key proteins related to atherosclerosis and dysfunction of lipid metabolism were also characterized.METHODS: Two-dimensional gel electrophoresis and mass spectrometry were used to analyze the differential displayed proteomics of 5-week-old double-gene mutants and wild type mice fed a regular chow for 2 weeks.RESULTS: Approximately (928±15) spots and (1 017±50) spots were detected in apoE-/-/LDLR-/- mice (n=3) and WT mice livers (n=3),respectively.The average matched ratio was 78.7% and 83.2%.The differential expression analysis showed that the matched spots between apoE-/-/LDLR-/- mice and WT mice existed.Compared with the wild type,108 spots were not matched in apoE-/-/LDLR-/- mice.10 over expression spots (>5 fold) and 45 lower expression spots (>5 fold) were noted.Six significant differential proteins in gel were identified by LTQ-ESI,e.g.endoplasmin precursor,acidic leucin-rich nuclear phosphoprotein 32 family member A,serotransferrin precursor,stress-70 protein precursor,fibronectin precursor,complement C3 precursor,fibrinogen gamma polypeptide.CONCLUSION: The protein profile of apoE-/-/LDLR-/- mouse liver exhibits significant difference compared to that of WT mice.The results imply that lipid metabolism relative polygenetic mutation contributes to the alteration of mouse liver protein expression profile,especially that lipid metabolism related perhaps participates in dysfunction in lipid metabolism during atherogenesis.     

Role of CXCR4 in changes of protein C system in ulcerative colitis mice

AIM: To explore the role of chemokine receptor CXCR4 in the pathogenesis of protein C system (PCS) in ulcerative colitis (UC).METHODS: In vivo, the mice were divided into control group and UC group. The macroscopic score, microscopic score and ulcer index were assessed. The mRNA levels and activity of myeloperoxidase (MPO), cyclooxygenase-2 (COX-2), stromal cell-derived factor-1α (SDF-1α) and monocyte chemotactic protein 1 (MCP-1) both in colonic tissue and plasma were determined. The expression and location of CXCR4, β-arrestin, p-JNK, endothelial cell protein C receptor (EPCR) and thrombomodulin (TM) were detected. The activity of protein C (PC) and protein S (PS) was measured in each group. In vitro, mouse colonic microvascular endothelial cells were isolated, cultured and identified. Both CXCR4-overexpressing and CXCR4-silencing colonic mucosa microvascular endothelial cells were constructed. The effects of SDF-1α on the protein levels of EPCR, TM, β-arrestin and p-JNK, and on the activity of PC, PS and activated protein C (APC) were observed.RESULTS: Compared with control group, UC mice showed increased gross score, histopathological score and ulcer index (P<0.05). The mRNA levels and activity of MPO, COX-2, SDF-1α and MCP-1 in colon and plasma were increased (P<0.01). The protein levels of CXCR4, β-arrestin and p-JNK were up-regulated, EPCR expression was down-regulated in colon, and the activity of PC and PS in plasma was decreased (P<0.05 or P<0.01). CXCR4 overexpression further aggravated SDF-1α-induced PCS inhibition in colonic mucosa microvascular endothelial cells, and further up-regulated the protein levels of β-arrestin and p-JNK (P<0.05).CONCLUSION: PCS is inhibited in UC. CXCR4 is involved in the regulation of PCS inhibition by mediating chemokines and acting on colonic mucosa microvascular endothelial cells through β-arrestin-JNK pathway.     

Differential proteomic analysis of human lung adenocarcinoma cell line and bronchial epithelium cell line

AIM: The comparative analysis of total proteins expressed differentially between adenocarcinoma cell line A549 (a human lung adenocarcinoma) and normal cell line HBE (a human lung bronchial epithelium) was conducted to search the proteins involved in tumorigenesis and potential biomarkers of diagnosis or prognosis. METHODS: The proteins of dramatic differential expression were screened in adenocarcinoma cell Line A549 and normal cell line HBE by using immobilized pH gradient-two dimensional polyacrylamide gel electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry. Furthermore, the differential expressed proteins were confirmed by the method of Western blotting. RESULTS: Compared with the two cell lines, 21 differential expressed proteins were found and their functions involves in cell metabolism, protein modification, cell motility, protein trafficking and signal transduction. The up-regulation of heat shock protein beta-1 (HSPB1) in A549 cells was identified and confirmed. CONCLUSION: These results suggest that dramatic differential proteomic expression exists between the two cell lines. The high level of HSPB1 might play an important role in the process of tumorigenesis.     

Effects of ginsenoside Rb1 on proteome from hippocampus of rats with epilepsy induced by Coriaria lactone

AIM: To explore the mechanism of development of Coriaria lactone (CL)-induced epilepsy and the neuroprotective effects of ginsenoside Rb1 (GRb1) on the process of epilepsy by identifying the proteins related to CL and GRb1 in rat hippocampus with two-dimensional electrophoresis (2-DE) technology.METHODS: The rat model of epilepsy was induced by CL. The rats in control group, CL epileptic group and GRb1 +CL epileptic group were decapitated and the hippocampus were collected. Two-dimensional electrophoresis was applied to separate the proteins from each group. Analysis of 2-DE maps was used to determine differential expression of proteins by ImageMaster 2D Platinum 5.0, and some differentially expressed protein spots were picked up for further identification by matrix-assisted laser sorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).RESULTS: Six proteins were successfully identified. Among these, the expression of brain creatine kinase, endophilin-A1 and UPF0628 protein C10orf96 homolog was lower in GRb1+CL epileptic group than those in CL epileptic group. The expression of cytochrome P-450, phosducin-like protein and bridging integrator 3 protein was lower in GRb1+CL epileptic group than those in control group.CONCLUSION: The protein expression profiles are significantly different among control group, CL epileptic group and GRb1+CL epileptic group. The identified proteins may be related to the neuroprotective effects of GRb1. Among these, brain creatine kinase, endophilin-A1 and UPF0628 protein C10orf96 homolog may be related to CL-induced seizures.     

Comparative proteomic analysis of lens in congenital inherited cataract mice

AIM: To separate total lens proteins of congenital inherited cataract in mice and to observe the alteration of proteins after gene mutation.METHODS: We studied the mice with a spontaneous mutation of crystallin gamma S (Crygs) transmitted as a recessive trait. Total proteins were extracted and separated using immobilized pH gradient (IPG), two-dimensional electrophoresis (2-DE) and colloidal Coomassie brilliant blue (CBB) staining. The image analysis was carried out using PDQuest 7.30 software package. Several significantly differential proteins in gel were identified by matrix assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS). RESULTS: As the 882 μg sample was added, we detected (417±53) spots and (370±41)spots in cataract and normal lens, respectively. As the 190 μg sample was loaded, we detected (60±7) spots and (57±5) spots in cataract and normal lens, respectively. Seven kinds of differential proteins were identified, including BFSP/filensin, γS, γF, βA1, βB1, βB2 and αB. In crystalline lens of mutant mice, γS and beaded-filament structure protein (BFSP/filensin) were not detected. γF was down-expressed (＜4 fold) while βA1, βB1, βB2 and αB were over-expressed (＞4 fold) in mutant cataract. The latter proteins were less MW than normal, suggesting that they were possibly truncated.CONCLUSION: 2-DE and mass spectrometry can help to assess and analyze the function of proteins as a novel approach. The mutant Crygs gene can lead to the abnormity of γS crystallin, which can induce the changes of skeletonal protein (BFSP/filensin) and other crystallins (γF, βA1, βB1, βB2 and αB), and then evoke cataract secondarily.     

葡萄着色期果皮蛋白质组分析

为了研究葡萄不同着色期果皮中蛋白质表达特征,以着色初期、中期和后期等3个着色时期的葡萄果皮为研究对象,运用2-DE、MALDI-TOF/TOF-MS质谱技术以及生物信息学方法对差异蛋白进行分析,结果表明:(1)双向凝胶电泳显示,果皮着色3个时期有1050个高度重复的蛋白点,差异显著的蛋白点有162个,其中108个蛋白得到鉴定,有87个蛋白映射到葡萄蛋白质组数据库中;3个时期均表达的差异蛋白有20个,且随着果皮颜色加深,差异蛋白数量呈上升趋势。(2)GO分析显示,ATP合成酶β亚基(ATPβ)极显著富集于磷酸核糖代谢过程,对着色初期果皮生理代谢的能量需求具有重要意义。(3)KEGG分析表明,碳代谢、生物固碳、磷酸戊糖、氨基酸生物合成等代谢通路在果皮着色的不同时期显著富集。(4)qRT-PCR分析显示,S–腺苷甲硫氨酸合成酶基因(VvMETK4)在果皮着色后期表达量最高。     

Differential analysis of serum proteomics in Crohn's disease treated with infliximab

AIM: To identify the serum proteins that might serve as biomarkers for predicting mucosal healing (MH) in the patients with Crohn's disease (CD) treated with infliximab (IFX). METHODS: We collected serum samples before treatment (0 week, group A) and 14 weeks after treatment (group B) from 7 CD patients with IFX treatment who had achieved MH, as well as the serum samples from 7 CD patients who had not achieved MH (0 week, group C; 14 weeks, group D). Two-dimensional fluorescence difference gel electrophoresis was applied to analyze and compare the results of serum profiles between groups A and B, C and D, A and C, B and D. Matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and bioinformatics tools were utilized to preliminarily identify and figure out the differentially expressed proteins. RESULTS: (1) In total, there were 44 differentially expressed spots, 36, 3, 10 and 31 differentially expressed spots were detected while comparing A with B, C with D, A with C and B with D, respectively. (2) Among those spots, 17, 2, 2 and 15 proteins were identified, respectively. In total, there were 19 differentially expressed proteins, including apolipoprotein E, apolipoprotein A-I, complement factor H, and so on. (3) Protein functional association networks were carried out based on STRING database. CONCLUSION: The serum protein profiles obviously change after IFX treatment in MH CD patients, and the serum protein profiles of MH patients are different from that of non-MH patients after IFX treatment. The 19 proteins we identified may serve as potential biomarkers for predicting MH in CD patients with IFX treatment.     

Identification of UCHL1 as a potential biomarker for human gastric adenocarcinoma by subcellular proteomics

AIM: To search the biomarker for illustrating the pathogenesis of human gastric adenocarcinoma by differential proteome analysis of the cytosol of human gastric adenocarcinoma cell line SGC7901 and the corresponding immortalized normal gastric gland cell line GES. METHODS: The differential expression of cytosol protein between SGC7901 and GES cells was identified with subcellular protein separation and two-dimensional SDS-PAGE combined with MALDI-TOF-MS. The differential proteins were confirmed by Western blotting and semi-quantity RT-PCR. RESULTS: Ten repeatable differential proteins were identified. Compared with the expression level in normal gastric cell line GES, ubiquitin carboxyl-terminal hydrolase L1(UCHL1) was obviously down-regulated one both at protein and mRNA levels in gastric adenocarcinoma cell lines of SGC7901, AGS, BGC823 and MKN45. The expression of UCHL1 was also far lower in gastric cancer tissues than that in the corresponding normal tissues. CONCLUSION: Our finding suggests that UCHL1 has potential value as a biomarker for the diagnosis of gastric adenocarcinoma.     

Ischemia reperfusion-induced proteomic changes in rat skeletal muscle

AIM: To investigate ischemia reperfusion (I/R)-induced proteomic changes in rat skeletal muscle. METHODS: Healthy male Wistar rats were randomly divided into two groups as follows (n=6): sham group and I/R group. I/R of right hind limb was induced by 4 h ischemia followed by 24 h reperfusion. The 2-DE was applied to separate the proteins extracted from skeletal muscle tissue at the end of experiment, followed by Coomassie Brillant blue R-250 staining. Computer image analysis was used to determine the differential expression of proteins between the two groups, and 7 protein spots expressed differentially were picked out and subjected to in-gel digest and MALDI-TOP for identification. RESULTS: 354±13 proteins were detected and the match rate was (78.7±1.4)%. 10 proteins displayed significant changes after I/R, of which, 6 proteins increased and 3 proteins decreased in expression. Moreover, 2 spots in I/R group were observed, only 1 spots of which in control. 5 proteins were identified after mass spectrometry. Mitochondrial aldehyde dehydrogenase (ALDH) precursor, heat shock 27 kD protein (HSP27), an unnamed protein product (increased in I/R group), α-actin (decreased in I/R group), and nuclear transport factor 2 (NTF-2) W7a mutant were found in I/R group. CONCLUSION: I/R injury induced differential proteomic changes in rat skeletal muscle. ALDH, α-actin and HSP27 expression, and NTF-2 mutation are involved in I/R injury.     

Analysis of serum biomarkers in human knee osteoarthritis

AIM: To analyze the proteomic components of the sera from knee osteoarthritis patients and normal people, and to search proteins that might serve as serum biomarkers for osteoarthritis diagnosis, treatment or pathogenesis. METHODS: Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) was applied to analyze the sera obtained from the patients with knee osteoarthritis (n=4) and normal controls (n=4). The differentially expressed proteins were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Western blotting analysis was applied to confirm the results. RESULTS: Comparative proteomic data of serum from the patients with osteoarthritis was successfully obtained. Eight differentially expressed protein spots were observed. Five up-regulated and 3 down-regulated proteins were identified. Western blot analysis confirmed that α₂-macroglobulin was increased. CONCLUSION: There are significant differences between serum proteins obtained from the patients with knee osteoarthritis and normal controls. α₂-Macroglobulin might be utilized as potential biomarkers for the diagnosis and treatment of osteoarthritis.     

北海道黄杨叶片应答低温胁迫的蛋白质组分析

 采用双向电泳的蛋白质组学方法分析了北海道黄杨叶片蛋白质对低温胁迫的应答反应。北海道黄杨植株在室外种植，从2008年10月—2009年2月每月提取叶片总蛋白。在考马斯亮蓝染色的2-DE胶上发现36个表达量显著差异蛋白点，其中通过质谱鉴定出19个蛋白质，包括已知的和新鉴定的低温胁迫相关蛋白质。这些鉴定出的蛋白质涉及多个生理过程，如能量与代谢、调控、防御应答等，同时这些蛋白质的表达模式与生理生化变化模式相一致。     

Gene and protein alterations in the hippocampus of rats with temporal lobe epilepsy

AIM:To examine the expression profiles of both genes and proteins in hippocampus of rats with temporal lobe epilepsy (TLE) for revealing the molecular mechanisms of TLE and looking for the candidate targets and new therapeutic approaches in clinical practice.METHODS:Rat temporal lobe epilepsy was induced by administration of lithium chloride and pilocarpine (LiCl-PILO).The expression spectra of genes and proteins were constructed through the techniques of cDNA microarray,two-dimensional (2D) electrophoresis and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Subsequently,the differentially expressed genes and proteins were identified and analyzed.RESULTS:There were 192 genes of differential expression observed in hippocampal tissues of LiCl-PILO-induced temporal lobe epilepsy,and 159 genes have been registered in Genbank database,in which 84 genes were up-regulated while 75 genes were down-regulated.78 protein spots of differential display were screened out,in which 31 proteins were detected to be down-regulated and 47 were up-regulated.Finally,5 proteins were identified.CONCLUSION:These genes and proteins found in our study may play pivotal roles in the pathogenic mechanisms of epilepsy and may promise new therapeutic targets for refractory epilepsy in the future.     

Comparative proteomics of myocardial mitochondrial proteins in aging rats

AIM:To identify the proteins related to aging in the mitochondria of the heart. METHODS:Mitochondria were isolated from the hearts of adult (10-week) and old (12-month) rats (n=3). Total proteins extracted from the mitochondria were applied to the two-dimensional gel electrophoresis. The differentially expressed protein spots evaluated by a software were subjected to in-gel digestion, and analyzed by the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). ATP content in the heart was also measured. RESULTS:Eighty-four differentially expressed protein spots were observed, and fifteen of them were analyzed by MALDI-TOF- MS. Four proteins including creatine kinase, peroxiredoxin 2, Tu translation elongation factor and isocitrate dehydrogenase were up-regulated and 11 proteins including succinate dehydrogenase, malate dehydrogenase, aconitate hydratase, NADH dehydrogenase, ATP synthase H+ transporting, ATP synthase beta chain, heat shock protein 60, glucose-regulated protein 78, prohibitin, Aldehyde dehydrogenase 2 and voltage-dependent anion channel exhibited down-regulation in the aging group compared to the adult one. ATP content in the heart of old rats was significantly reduced as compared to that in adult rats (n=5, P<0.01). CONCLUSION:Significant changes in the mitochondrial protein expression in the aging heart were identified by the 2-DE electrophoretogram with high resolution and reproducibility. The functions of these identified proteins need to be further investigated.     

Identification and analysis of differentially expressed proteins during cotyledon embryo stage in longan

The cotyledon stage is a crucial developmental stage during longan embryo development. Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF/TOF-MS) were conducted to separate and identify proteins expressed in the longan cotyledon embryos at different development stages. A total of 28 proteins that exhibited regulated expression were successfully identified with a protein identification success rate of 72.2%. The 28 proteins were assigned to six functional classes based on their putative biological functions: energy and metabolism (21%), secondary metabolism (18%), protein metabolism (21%), cell division (11%), antioxidation (4%), nucleic acid metabolism (4%), hormonal regulation (4%) and unknown proteins (18%). Interestingly, three enzymes involved in flavonoid biosynthesis, whose expression in embryos had not been observed previously were clearly up-regulated during the cotyledon stage of embryo development in longan. Identification and analysis of the 28 proteins would shed new lights on further understanding of the biochemical and physiological processes of the embryo development at cotyledon stage in longan.     

Proteomic analysis of the effects of tumor necrosis factor-α on endothelial cells

AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-α in endothelial cells, and further explore the potential molecular mechanism of TNF-α on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-α treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-α stimulation, and 1 spot was only detected in TNF-α activated cell gels. CONCLUSIONS: Decreased the expression of ecNOS by TNF-α might result in decreased NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-α activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-α injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-α.     

Study on expression of long non-coding RNA in colon cancer tissues and adjacent tissues

AIM: To screen the differentially expressed long non-coding RNA (lncRNA) in colon cancer, and to explore its expression in colon cancer tissues and adjacent tissues. METHODS: The "Colon adenocarcinoma:Person neoplasm cancer status" which consisted of 36 cases of colon cancer tissues and 29 cases of normal colonic tissues was downloaded from the lncRNAtor database. The candidate genes were selected from these differentially expressed lncRNAs based on artificial criterion (P<0.01; fold change ≥ 2 or<0.5) and then validated by real-time PCR in 60 pairs of colon cancer tissues and adjacent tissues. RESULTS: A total of 50 lncRNAs were differentially expressed in colon cancer tissues, including 28 up-regulated and 22 down-regulated (P<0.01). The verifying results displayed that HNF1A-AS1 and ZDHHC8P1 were up-regulated (P<0.01), and SUZ12P expression was down-regulated (P<0.05), but the expression of AC069513.3 was not statistically significant between colon cancer tissues and adjacent tissues. The abilities of HNF1A-AS1, ZDHHC8P1, SUZ12P and AC069513.3 to discriminate the colon cancer from normal adjacent tissue by the ROC curve with an AUC of 0.729 (sensitivity 78%, specificity 67%), 0.617 (sensitivity 68%, specificity 55%), 0.689 (sensitivity 66%, specificity 55%) and 0.518 (sensitivity 52%, specificity 48%) were observed. CONCLUSION: Long non-coding RNA HNF1A-AS1 and ZDHHC8P1 are up-regulated and SUZ12P is down-regulated in colon cancer tissues, suggesting that they may be involved in the pathogenesis of colon cancer.