魔芋叶片中4种总RNA提取方法的比较

本研究分别利用TRIzol法、异硫氰酸胍法、改良的CTAB法及试剂盒这4种方法提取魔芋叶片的总RNA,并用紫外光谱和琼脂糖凝胶电泳对各个提取效果进行鉴定。反复试验的结果表明:TRIzol法难以提取魔芋叶片总RNA,异硫氰酸胍法提取的总RNA浓度低、杂质较多且有部分降解,这两种方法不适宜用于魔芋叶片总RNA提取;试剂盒方法提取的总RNA产率和纯度较高,但稳定性差、成功率低且成本较高;改良的CTAB法利用PVP和β-巯基乙醇去除酚类物质,水饱和酚-氯仿去除蛋白质,醋酸钾去除多糖,该方法提取得到的总RNA的纯度较高,OD260/OD280值为1.893,而且效果稳定,有良好的可重复性。因此,我们认为改良的CTAB法是一种简便、快速且经济的提取魔芋叶片总RNA的方法。     

A New Dynamic Method for Calculating NPV

NPV have been widely applied. A majority of the existing calculating methods of cash flow are the static method or the expectation method. It is more reasonable to regard future cash flow as random variable which has certain probability distribution, which shifts with time. But the research about it has been introduced rarely. Through introducing some kinds of existing methods and considering the feature of Markov method, a dynamic method which is used in solved the distributing of indeterminate feature cash flow is showed. So a new kind of dynamic calculation method about NPV is obtained. The method is applied in the calculation to future cash flow of the certain highway investment project of China. The result shows that this method is feasible, effective.     

天冬氨酸衍生物在土壤重金属污染治理方面的研究进展

本研究综述了土壤重金属污染的研究进展,对天冬氨酸的2种绿色衍生物聚天冬氨酸(PASP)和亚氨基二琥珀酸(IDHA)进行了相关介绍,并针对其作用特点总结了2种物质在土壤重金属污染治理方面的相关研究和应用进展。最后,笔者提出了在土壤重金属污染治理方面这2种化合物的应用前景和使用方向,认为这2种化合物可以将化学法与生物法结合起来进行土壤重金属污染的治理,能有效提高重金属治理的速率,应用前景广阔。     

Improved General Limit Equilibrium Method for Slope Stability Analysis

Based on the theory of ultimate equilibrium, an improved General Limit Equilibrium method (IGLE) is studied, and it is implemented in Rslope software. IGLE can simulate various limit equilibrium methods and takes into account external factors(water、tensile crack、external load) and reinforcing measures(anchor、anchor-cable、geotextile、soil nail). Imbalance thrust force method is simulated in this study, and analysis on precisions of safety coefficient and residual pushing force under two conditions of imbalance thrust force which are strength reserve method and overload method respectively by using IGLE is conducted. The study also promotes an effective usage recommendation. Aiming at the problem that engineer is easy to confuse residual pushing force with inter-slice force, comparative analysis on horizontal components of inter-slice force and residual pushing force is conducted. The results show that there is no direct correlation between them. Then the results of calculation examples of Rslope are compared with other commercial soft wares, such as slide, SLOPE/W. Specifically,the comparison includes automatically searched safety factor of circular slip surface, safety factor of sliding surface and safety factor of reinforcement. The results have a good consistency. IGLE is of practical significance for real applications.     

Impact of saturation on the characteristic of inductance of permanent magnet synchronous motors

Since rotor structures of the IPMSM are different from the common motor, inductance parameters are hard to be accurately calculated by the traditional calculation method. A new analytical method, which is called the fixed permeability method (FPM), is used to calculate the inductance parameters and the magnet flux λm of IPMSM. The results calculated by the FPM and the conventional finite element method (FEM) are compared and the reasons which caused the different results are analyzed. The impacts of saturation and cross coupling on the inductance and the magnet flux λm and the impact of the width of the bridge on the inductance are studied. On the basis of the analysis, the influence factors and the rules of the inductance parameters of IPMSM are summarized, which are good for analysis and design of PMSM.     

Study on the Numerical Manifold Method Remeshing Technique and Its Application in metal Forming Processes

The criterion for remeshing and the automatic generating method of new mesh system are studied. The parameter transmitting method for the information transferring between new and old meshes is put forward by using Sherpard shape function. A general remeshing method suitable for two dimensional rigid plastic analysis is derived. The numerical examples show that the method is of high presicion and efficiency.     

山西草地螟发生预测模糊近似推理模型

模糊近似推理方法是模糊集理论应用前景十分广泛的一种方法.我们用此法为山西草地螟发生预测建立了一个模糊近似推理模型,初步试用效果满意.本文在模型的预报输出中引用了特征展开近似推理方法,大幅度地简化了运算过程;并且使得模型可以带入较多的因素.     

FDM for Large Strain Consolidation of Multi-Layers Super Soft Ground

Follow in precursory relating documents, several equations of one dimension Large Strain Consolidation (LSC) and consolidation coefficients were discussed firstly. Based on this, a Finite Difference Method (FDM) equation was derived, which was the physical described LSC equation with variable displacement. Moreover, the initiate and boundary condition were dispersed by FDM, and the boundary of different layers of super soft soil was disposed of continuity. Finally,nonlinear group equations of LSC FDM solution was deduced for multi layers super soft ground by repeated method, and a FDM program of 1D nonlinear LSC analysis was compiled. Finally, a case showed a satisfied analysis result by LSCFDM.     

雪莲果果粉生产工艺的探讨

以雪莲果为试验材料,研究了自然干燥、热风干燥、微波干燥、真空干燥4种不同干燥方法所制得的雪莲果果粉的品质。结果表明,热风干燥所制得雪莲果果粉的品质较好,口感细腻滑溜,清香爽口。     

细胸金针虫田间分布型及抽样技术

细胸金针虫幼虫在田间的空间分布,既符合负二项式分布,又符合当n大于某一值的奈曼分布.土壤10cm以上浅层分布基本与幼虫在整个活动深度上层内的空间分布相一致,春、秋期间,大部分幼虫在10cm以上浅层土壤内取食危害.幼虫的抽样调查可选在春、秋危害期于10cm以上土层内进行.本研究采取的几种简单随机抽样方法与实地虫量调查无显著差异,其中以平行线方法效果较佳.     

种粒大小选择对大豆种间杂交后代性状的影响

以两种类型大豆种间杂交组合Ｆ２至Ｆ４为材料,研究种粒大小集团选择效应及对其他性状的影响。表明：种粒大小是一个重要性状,大豆种间杂交后代随着向大粒方向选择,植株变矮,茎秆增粗,倒伏性降低,分枝数减少,产量性得以改善。在Ｆ２根据植株体表现进行种粒大小的分组定向选择,这种趋势能够有效地保持到高代。     

高粱原生质体的制备及转化方法研究

以高粱幼苗为材料,利用1%的纤维素酶Cellulose R10和0.75%的果胶酶Macerozyme R10处理高粱幼茎,对比不同的酶解时间,发现酶解7h原生质体浓度最高、质量最佳。以经过氯化铯密度梯度离心法获得的高纯度质粒对获得的原生质体进行转化,用激光共聚焦进行荧光观察,结果显示,GFP和YFP在全细胞表达。本研究首次建立了高粱以高粱幼茎为材料的原生质体制备及转化体系,其方法简单、高效,可以用于高粱基因功能研究。     

浅析荔枝蒂蛀虫测报方法之改进

结合当地气象资料和温控试验结果,应用田间调查观察和室内饲养观察进行荔枝蒂蛀虫的预测预报的改进和完善,并把测报技术用于实际生产中,建立荔枝、龙眼果园荔枝蒂蛀虫种群发生期的预测,其结果与实际发生期基本相吻合。     

烟草含钾量反射仪-钾离子试纸法快速测定研究初报

比较了反射仪—K 试纸法(K ts-fl)、原子吸收光谱法、ICP-aeS法测定烟株叶脉汁液中的K 含量的三种方法,确定反射仪—K 试纸法测定烟株钾含量的可行性及其最佳测定范围;在烤烟不同生育期,应用反射仪—K 试纸法对烟株叶片不同部位含K 水平的测定,确定其最佳测定时期及部位。研究结果表明,反射仪—K 试纸法与原子吸收光谱法、ICP-aeS法测定结果差异不显著,应用反射仪—K 试纸法进行烟株钾素快速诊断可行,且具有时间短,简单快速,易操作等优点。反射仪测定K 的稳定线性范围是0.30～0.9g/L;在不同时期对烟株不同部位测定研究表明,最佳诊断部位为烟株第二平展叶叶脉基部2cm段。     

SOC Definition of Battery Management System Used in Hybrid Vehicles

For the requirement of building up the battery management system (BMS) of hybrid vehicles(HEVs), the real time measurement and estimation of charge (SOC) of the Ni MH batteries are investigated, because the popular SOC definition is unsuitable under the condition of variable current discharge. The origin of this kind unsuitability are analyzed ,and the characteristics and existing problems of different kinds of SOC measuring methods are compared. On the basis of mentioned above, some corrections are given to the popular SOC definition, and the new conception of SOC is proposed. The corrected definition of SOC is suitable for the real time SOC estimation under the variable current condition of the batteries used in HEVs. Based on the corrected SOC definition, the computational model of the new SOC is built and simulated. Satisfactory result has been obtained from comparisons of the simulating results, which shows that the theoretical model provides a basis for optimizing the adaptation of power system to the hybrid vehicles (HEVs).     

改良Trizol法快速高效提取香石竹总RNA

因为多糖等大分子的污染,传统Trizol法难以从香石竹的叶片中获得完整RNA。本研究以香石竹为材料,对传统的Trizol法进行改良,成功地从香石竹花瓣和叶片中提取了总RNA,经琼脂糖电泳,紫外分光光度计测定总RNA完整性好,纯度大,花瓣的OD260/OD280=1.96,每0.1g花瓣可获得RNA61μg,叶片RNA的OD260/OD280=1.94,每0.1g幼叶可获得RNA33μg,花瓣的RNA产量普遍高于叶片。经改良Trizol法提取的被香石竹斑驳病毒侵染的植株的花瓣和叶片的总RNA,经RT-PCR获得了特异性条带,说明用改良Trizol法从香石竹花瓣和叶片中提取的RNA质量好、产率高、完整性强,完全适合于香石竹进一步的分子生物学研究。     

环介导等温扩增技术检测含有CaMV35S的转基因玉米

DNA环介导等温扩增技术是一种特异、灵敏、快速的新型基因检测技术.针对玉米表达载体的花椰菜花叶病毒35 S启动子(CaMV35S)的6个区域设计4种特异引物,对LAMP反应的MgSO4、dNTPs、Betaine、内引物、外引物各个成分进行了优化,此外还对LAMP和PCR两种不同方法的特异性进行了比较.建立转基因玉米花...     

BEM of Two-dimension Multiharmonic Equation and Its Applications

The paper discusses BEM of 2_dimension non_homogeneous multiharmonic equation.Under supposing non_homogeneous term is m_degree homonic,the integral in domain is transformed boundary integrals,and boundary integral equations are obtained correspondly.     

混选—混交法应用于棉花育种研究 Ⅰ.育种模式及效果

本文详细叙述了棉花混选—混交新育种方法的程序及其育种效果。在混选一混交后的群体中已选得一批新品系,其产量、品质、抗性等方面接近或超过中棉12。新品系1276和164参加了省级以上品种区域试验,表现较好。而同时采用常规的杂交育种法未能选到优良品系。不去雄混交法可保证较高比例异交率,从而使这种新育种方法具有较好的应用前景。     

Prediction Model of Exploitation Sink Using Ann and GIS

A new idea is put forward on researches of prediction method of coal mining subsidence supported by GIS, and a new method is given about quantitative prediction of mining subsidence by means of GIS and ANN(Artificial Neural Network).This paper has completed a lot of work concerning choosing factor,data processing, establishing and validating preliminary ANN prediction model. Further more,data processing is carried out by GIS software,and the BP training method is used for modeling the exploitation sink system. Subsequently,the error is qualitatively analyzed with considering the result of verification. Researches show that the ANN prediction model supported by GIS has theoretical feasibility and realistic significance in predicting complex exploitation sink system,and GIS and ANN possess wide application prospects in the prediction of exploitation sink.