Changes in the geometric structure of pores and aggregates as indicators of the structural degradation of cultivated soils

As shown by the example of loamy soils of the European territory of Russia, the geometric parameters of soil structure, along with the agrophysical soil parameters, should be taken into account for the comprehensive assessment of the physical degradation of cultivated soils. Different variants of the geometric transformation of soil structure in the plow horizons are analyzed. The shape and orientation of soil pores in thin sections prepared from undisturbed oriented soil samples are considered to be the main diagnostic indices. A computer-aided analysis of soil pores in thin sections made it possible to distinguish and characterize different levels of the geometric transformation of soil structure upon soil compaction: (a) without the disturbance of the shape and orientation of the aggregates, (b) with the transformation of soil aggregates from the crumb (granular) to the angular blocky shape, (c) with the development of a platy structure characterized by the predominantly horizontal orientation of the pores and aggregates, and (d) the complete disappearance of separate aggregates with the formation of a massive soil structure. The validity of the assessments of the geometric transformation of the soil pore space against the background of a considerable spatial variability in the geometric properties of soil structure in the plow horizon is discussed. The structural-functional specificity of the distinguished levels of the geometric transformation of soil structure is outlined.     

Microtomographic analysis of pore space in a virgin soddy-podzolic soil

The method of X-ray microtomography was applied to study pore space of a virgin soddy-podzolic soil at the natural soil water content. The morphometric parameters of the pores of more than 100 μm in diameter were determined in the vertically oriented undisturbed soil monoliths (d = 3 cm, h = 3–4 cm) from the genetic horizons of the most differentiated part of the soil profile (the AY, AEL, EL, BEL, BT1, and BТ2 horizons). A tendency for the horizontal orientation of these pores was found in all the horizons, except for the humus (AY) horizon. Isolated vesicular pores of different sizes were abundant in the eluvial part of the profile. Numerous recent and relict phytogenic channels were found in the intraped mass of the BT2 horizon. Differently directed interfaces of structural units in the soil horizons were visualized. Cluster analysis was applied to estimate differences between the genetic horizons with respect to their textures, aggregate sizes, and shapes of pores as seen in vertical two-dimensional X-ray images.     

Morphometric profiles of pore space in loamy soils of the forest and steppe zones of European Russia

A computer-based image analysis of vertically oriented thin sections was applied to study changes in the shape and orientation of fine soil macropores (d = 0.2−2.0 mm) in the profiles of soddy-podzolic soils and typical (migrational-mycelial) chernozems. Generalization of the obtained morphometric data was based on the theory of mereology, a scientific discipline studying the structure (part-whole relationships) of classified objects. As a first approximation, generalized data characterized archetypes of morphometric porespace profiles of the studied soils. The archetype of the pore-space profile of the soddy-podzolic soil consists of four components (meronyms) corresponding to the humus-accumulative, eluvial, textural (clay-illuvial), and transitional to the parent material (BC) horizons. Sharp boundaries between the upper horizons specify sharp changes in the studied meronomic indices of the shape and orientation of soil pores. The pore-space profile of the migrational-mycelial chernozem consists of two major components: specific pores in the granular dark-humus (AU) horizon and complex pore space of the BCA and BCca horizons that are poorly differentiated with respect to the shape and orientation of their fine macropores despite clear genetic differences between these horizons. Pore-space patterns in the lower (transitional to the parent material) horizons of the studied soils are characterized by the high degree of similarity (>75%). Pore-space patterns in the upper horizons of the studied soils are different; the level of their similarity does not exceed 24–41.5%. The results obtained in this study hold promise in the use of morphometric characteristics of the pore space in separate genetic soil horizons as meronyms composing archetypes of the pore-space profiles of different soils. Such archetypes may be used for diagnostic purposes as reference pore-space profiles of the particular types of soils.     

The over-estimation of clay and the under-estimation of pores in soil thin sections

The amounts of clay visible in four thin sections from paleosol horizons (clay contents 9–32%) were measured by point counting. These sections were used as references for visual estimates, without point counting, of the amounts of clay in 14 further sections. The measurements and estimates were recorded on pore-free and gravel-free bases so they could be related to gravimetric analyses. Amounts of clay measured and estimated by area in these thin sections were between 1.6 and 3.1 times those determined gravimetrically. The reasons for these differences are discussed. Firstly, clay in soil thin sections contains much pore space beyond the resolution of the optical microscope and has an apparent density dependent on the amount of submicroscopic pore space. An edge effect also causes over-estimation of clay in soil thin sections and under-estimation of visible pore space. Components (clay, mineral grains, pores) are usually sloped at their borders with each other within the standard 25–30 μm (three-dimensional) thin sections. Clay, for instance, may overcap a pore at its border, hiding some pore space. The need for true two-dimensional images of soil pore patterns in image analysis is indicated. A nomogram has been constructed, using apparent density, to allow estimation of clay in thin sections of soils with any clay content. Coarse/fine ratios can be determined more accurately and estimates of clay can also be recalculated to provide illuvial to total clay ratios that are more meaningful. It is recommended that the micromorphological identification criterion for the argillic horizon be reassessed and that 4% illuvial to total clay in a soil thin section be adopted as the diagnostic cut-off.     

One-dimensional image analysis of soil structure. 11. Interpretation of parameters with respect to four forest soil profiles

The one-dimensional (1-D) image analysis system described in the companion to this paper (Part I) was used to analyse horizontal sections of pore space (≥ 180 μm) in four forest soil profiles with impeded drainage. Parameters were interpreted with respect to structure in the images. Mean solid intercept length is inversely proportional to extent of pore space. It was longer for blocky structures than massive structures, which had extensive, fine pore space. It decreased with decreasing ped size, increasing development and increasing intrapedal porosity. The solid intercept length distribution separated the effects of intrapedal porosity. For blocky structures, the pore intercept length distribution indicated the sizes and quantities of fissures and intrapedal pores. All profiles had an horizon with blocky structure with lower porosity owing to greater distance between interpedal pore space and low extent of intrapedal pore space. The deeper, more massive horizons had extensive, fine pore space separating fine aggregates.     

Changes in the macro-pore structure of restored soil caused by compaction beneath heavy agricultural machinery: a morphometric study

Compaction can seriously degrade soil in modern agriculture. Soil that has been temporarily removed and stored is particularly sensitive to compaction when restored, although little is known about the structural changes in such soils under mechanical loads. We investigated the structural changes in a restored soil that had been gently cultivated for several years and then was trafficked by a heavy combine harvester, analysing the macro‐pore structure by quantitative morphometry of three‐dimensional microcomputed tomography images. Increased trafficking caused decreases in both the porosity and connectivity of the macro‐pores. The fraction of spherical pores (and thus the convexity of the pore space) and the mean pore separation were increased. Trafficking had no clear effect on the orientation of pores. While the mean pore diameter tended to decrease, biopores were more stable than interaggregate pores originating from the packing of soil aggregates. This is relevant for the development of structural stability in restored soils, as the macro‐pores consist mainly of interaggregate pores initially, whereas biopores develop and increase in proportion only gradually over time. Quantitative morphometry provides valuable morphological indices for the objective assessment of the macro‐pore structure and changes induced by compaction.     

Micromorphometric features of pore space in the plow horizons of loamy soils

A computer-based analysis of thin sections has been applied to study pore space in the plow horizons of loamy soils in European Russia and Ukraine. Differences in the morphology of soil macro-and mesopores are shown. It is argued that agrogenic impacts result in the convergence of the shape and orientation of macropores in plowed loamy soils of the forest, forest-steppe, and steppe zones. At the same time, this convergence is not observed for the soil mesopores.     

Morphological determination of pore connectivity as a function of pore size using serial sections

The geometry of pore space in soil is considered to be the key in understanding transport of water, gas and solute. However, a quantitative and explicit characterization, by means of a physical interpretation, is difficult because of the geometric complexity of soil structure. Pores larger than 40 μm within two soil horizons have been analysed morphologically on 3-dimensional digital representations of the pore space obtained by serial sections through impregnated specimens. The Euler-Poincaré characteristic has been determined as an index of connectivity in three dimensions. The pore connectivity is quantified as a function of the minimum pore diameter considered leading to a connectivity function of the pore space. Different pore size classes were distinguished using 3-dimensional erosion and dilation. The connectivity function turned out to differentiate between two soil materials. The pore space in an upper Ah horizon is intensely connected through pores between 40 and 100 μm, in contrast to the pore space in the AhBv beneath it. The morphological pore-size distributions were compared to the pore-size distribution obtained by water retention measurements. The discrepancy between these different methods corresponds to the expectation due to pore connectivity.     

A casting method for the three-dimensional analysis of the intraprism structural pores in vertisols

The structural voids in vertisols contain easily available water for plants and their volume can be calculated from the shrinkage curve. Access by plants to that water depends also on the geometric arrangement of the pores so that the water can flow through them. We have devised a method for studying the structural porosity by casting the pores in resin. The intraprism pore space of wet soil clods is impregnated with a UV fluorescent polyester resin under vacuum. When this has set we use the swelling properties of the clay to separate the clay matrix from the resin. A cast so obtained is the real three-dimensional solid reproduction of the structural porosity. This representation of the pore system is easier to study than results from computerized reconstitution of the three-dimensional space from two-dimensional images of soil in thin sections. Channels, packing pores and planar voids can be observed directly in three dimensions as the method saves the integrity and continuity of pores as small as 10 μm in diameter. The geometry of the cast shapes agrees with the interpretation of shrinkage and moisture characteristic curves. The method offers direct qualitative observation of pore organization and volume measurements of the intraprism structural porosity in vertisols.     

Anisotropy of the properties of some anthropogenically transformed soils of podzolic type

It is shown that the horizons and profiles of anthropogenically transformed soils of podzolic type—light typical agrozems, typical texture-differentiated soils developed from glaciolacustrine loamy sands and clays and from noncalcareous mantle loams, agrosoddy deeply podzolic soils developed from noncalcareous mantle loams, and agrosoddy shallow-podzolic soils developed from noncalcareous mantle loams and from calcareous loams underlain by ancient glaciolacustrine loams and clays—are characterized by some anisotropy of most of their properties. The highest anisotropy is typical of the field water content, bulk density, and total porosity. The coefficients of anisotropy (gradients) calculated for the separate horizons as the ratios between the values of the properties measured in the horizontal and vertical directions (k = P_horiz/P_vertic) of these properties are much higher than those of other soil properties. The coefficient of anisotropy of the soil profile (K) is suggested as the coefficient of correlation between the values of a given property determined in the horizontal and vertical soil sections. For the considered properties, K varies from 0.4 to 0.6. For other soils properties, such as the solid phase density, the electrical resistance determined in a laboratory and in the field, and the organic carbon content, the coefficients of anisotropy are close to 1.0. The clay content has an intermediate anisotropy. The values of anisotropy and its direction (gradient) should be taken into account upon the assessment of the soil physical properties and the processes controlling them; this is particularly important in the study of soil transformation. The revealed regularities of the soil anisotropy make it possible to suggest a new interpretation of the data on the distribution of water and energy in soil profiles.     

Anisotropy of pore functions in structured Stagnic Luvisols in the Weichselian moraine region in N Germany

In order to determine if soil hydraulic properties present a direction‐dependent behavior, undisturbed samples were collected at different horizons and orientations (vertical, diagonal [45°], and horizontal) in structured soils in the Weichselian moraine region in northern Germany. The water‐retention curve (WRC), the saturated hydraulic conductivity (k_f), and the air permeability (k_a) were measured. The air‐filled porosity (?_a) was determined, and pore‐continuity indices (k_a/?_a, k_a/?_a², N) and blocked porosities (?_b) were derived from the relationship between k_a and ?_a. The development of soil structures with defined forms and dimensions (e.g., platy by soil compaction or prismatic up to subangular‐blocky by swelling–shrinkage processes) and the presence of biopores can induce a direction‐dependent behavior of pore functions. Although the pore volume as a scalar is isotropic, the saturated hydraulic conductivity and air permeability (as a function of air‐filled porosity) can be anisotropic. This behavior was observed in pore‐continuity indices showing that the identification of soil structure can be used as a first parameter to estimate if hydraulic properties present a direction‐dependent behavior at the scale of the soil horizon.     

A new approach for determining effective soil hydraulic functions

We investigated the possibility of inferring effective hydraulic properties of soil from the structure of the pore space. The aim was to identify structural properties, which are essential for water flow, so that physical experiments may be replaced by direct morphological measurements. The pore structure was investigated in three dimensions by serial sections through impregnated samples. The complex geometry of pore space was quantified in terms of two characteristics: pore-size distribution and pore connectivity. Only pores larger than 0.04 mm were considered. The results were used as input parameters for a pore-scale network model. The main desorption branch of the soil-water characteristic and the corresponding hydraulic conductivity function of the network model were calculated by numerical simulation. The simulation results, which are exclusively based on morphological investigations, were compared with independently measured results from a multi-step outflow experiment. This approach was demonstrated for two centrasting soil materials: the A and B horizons of a silty agricultural soil. The simulations were close to the experimental data, except for the absolute values of the hydraulic conductivity. The pore-size distribution and pore connectivity govern the shape of hydraulic functions and the applied morphometric methods are suitable for predicting essential characteristics of hydraulic soil properties.     

Micromorphometric and micromorphological investigations of a clay loam soil in viticulture under zero and conventional tillage

Porosity, pore size distribution and the orientation pattern of pores were measured on thin sections prepared from undisturbed soil samples by means of electro-optical image-analysis. Total porosity was significantly higher at all sampling times in conventionally tilled plots, but the proportion of pores ranging from 30 to 500 μm, which are considered the most important both in soil-water-plant relationships and in maintaining a good soil structure, was higher in no-tilled plots. Modifications of pore orientation pattern were also observed. Micromorphological observations revealed some differences between the two series of soil samples; the formation of surface soil crusts was strongly reduced in no-tilled plots.     

A comparative study of three methods of water removal prior to resin impregnation of two soils

The water in replicate samples from sub-surface horizons of a clayey and a fine-silty soil was removed either by oven-drying, freeze-drying or by acetone-replacement prior to resin impregnation. Pore space photograms (pores >60 μm diam.) from thin sections were analysed on a Quantimet 720 image analysing computer following each drying technique. During oven- and freeze-drying the clayey soil contracted considerably and subsequently satisfactory impregnation of these samples was either difficult or impossible. This macro-shrinkage caused a decrease in number and lengths of most planar pores and a decrease in size and number of all intra-aggregate pores. The loss in pore space and continuity obviously affected resin impregnation. The clayey samples in which water was replaced by acetone, and all samples of the silty soil, however pretreated, showed no measurable macro-shrinkage and all impregnated well. Oven-drying the silty soil appears to increase the porosity, causing an increase in size of all pores. A mechanism for this apparent enlargement is proposed. It is evident that acetone-replacement of the soil water prior to resin impregnation is the best of the three methods used here and should be seriously considered when image analysis is to be carried out on resin-impregnated blocks from soils of similar or related textures.     

Experimental simulation of the results of clay translocation in the B horizons of soils; the formation of intrapedal cutans

A series of laboratory experiments designed to simulate the formation of intrapedal cutans was carried out through repetitive leachings of undisturbed blocks of soils (6 × 3 × 4 cm³) by suspensions (0.3% concentration) of clay-sized particles from two commonly occurring Bt horizons in southern England. After leaching, thin sections were cut and point-counted, thus enabling the visual recognition, replication and potential quantification of newly formed intrapedal pore and grain cutans. They all exhibit morphological, physical and optical properties diagnostic of illuviation cutans, and can be differentiated from inherited cutans on these and on spatial grounds. A general relationship between numbers of newly formed cutans and increasing leaching cycles was observed, and experimental support obtained from these soils for the significance of the complex, three-dimensional spatial pattern of tubular pores in the translocation of clay-sized materials and the formation of these argillic, Bt horizons. This study highlights both the value and difficulties of designing laboratory-based, soil/water/gravity studies in pedology.     

THE MEASUREMENT AND CHARACTERISATION OF VOIDS IN SOIL THIN SECTIONS BY IMAGE ANALYSIS. PART II. APPLICATIONS

Image analysis was used to measure and characterise the voids in two sets of soil samples; (i) a surface water gley soil of the Deighton series and (ii) soils from a compaction experiment. The results show that the Deighton soil contains two impervious horizons separated by a much more porous horizon. In all the horizons most of the pore space is due to pores >180μm diam. In the case of the soils from the compaction experiment compaction was shown to change the shape, orientation and size distribution of the pores considerably. The range of measurements demonstrates that image analysis is applicable to several areas of soil research.     

3D-visualization and analysis of macro- and meso-porosity of the upper horizons of a sodic, texture-contrast soil

The lower E and upper B horizons of sodic, texture-contrast soils are a formidable barrier to most annual and many perennial crops. The research presented here is part of a wider study into the nature of subsoil constraints to root exploration. The aim of this study was to characterize in three dimensions the macro- and meso-porosity across the E horizon–Btn horizon interface of a sodic, texture-contrast soil using X-ray computed tomography (CT). Intact soil cores of 50-cm length and 15-cm diameter were scanned with a medical CT X-ray machine. The pore volume reconstructed from these scans had a resolution of 0.3 × 0.3 × 0.4 cm (in the x, y, z dimensions, respectively). This resolution allowed visualization and quantification of the macroporosity of the intact cores. Undisturbed samples of 1.5-cm diameter and 4-cm length were carefully excised from the interface and scanned with micro-CT X-ray equipment. The reconstructed pore volumes had an isotropic resolution of 19 μm that allowed analysis of the mesoporosity just on the boundary between the E and Btn horizons. Mesoporosity decreased across the interface and increased lower in the Btn horizon. The distribution of the pores at the macro- and meso-scales showed the importance of the smaller pores in the A and E horizons, whereas most of the porosity in the Btn horizon was attributed to the larger pores. Pores in this sodic, texture-contrast soil were not distributed homogeneously at either the macro- or meso-scale. A greater proportion of the pores in the E–B interface were horizontal than in the upper A1, upper E and lower Btn horizons. Some 'coiling' of the pores was also apparent in the interface. The shape of some pores (long tubular pores) suggested formation by roots as they drilled through the soil. The orientation of these pores was a function of physical (and possibly chemical) impedance at the interface.     

Tomographic method of studying soil pore space: Current perspectives and results for some Russian soils

The methods and results of tomographic soil studies are discussed. A brief history of the development of the tomographic method and its application in soil science are described, and the major results obtained with the use of this method are outlined. An experience of the application of X-ray microtomography for studying the structure of undisturbed samples of some Russian soils is also considered. Three-dimensional images of soil aggregates and soil pore space with a resolution of 15.8 ??m have been obtained for the upper horizons of gray forest and soddy-podzolic soils. On the basis of these data, the merits and demerits of the tomographic method in comparison with the traditional method for studying soil pedality and pore space in thin sections are discussed. Certain advantages of the tomographic method provide good possibilities to study soil processes at a qualitatively new level. Priority directions of the development of tomographic studies in soil science are outlined.     

Rheological Properties and Tomographically Determined Pore Space of Undisturbed Samples of Typical Chernozems and Soddy-Podzolic Soils

Coupled studies of pore space and rheological behavior of undisturbed samples from soddypodzolic soils (Albic Glossic Retisols (Loamic, Aric, Cutanic)) of Moscow oblast under forest and under cropland and from typical chernozems (Haplic Chernozems (Loamic, Aric, Pachic)) of Kursk oblast under oak forest, shelterbelt, and cropland were conducted. Soil pore space was investigated using a Bruker SkyScan 1172 G (Belgium) microtomograph, and 3D models of pore space were constructed. The total pore space (in percent of the volume of analyzed samples) and the volumes of open and closed pores were determined from these models. The nondestructive tomographic method made it possible to analyze the rheological properties of soils for the same samples using the amplitude sweep method on an MCR-302 (Anton Paar, Austria) rheometer. The following parameters of the rheological behavior were determined: storage modulus in the range of linear viscoelastic behavior, the range of linear viscoelastic behavior, and the range of plastic behavior. A joint analysis of the rheological properties and morphometric characteristics of the undisturbed samples of soddy-podzolic soils and chernozems demonstrated the dependence of the rheological behavior of these soils on their physicochemical properties and pore space structure reflecting the differences in the genesis and physical and chemical properties of soil horizons. The correlation analysis attested to direct (positive) relationships between the values of the total and open tomographic porosities, the range of linear viscoelastic behavior, and the deformation upon the destruction of soil structure. Negative relationships were found between the values of open and total porosity and the structural strength of the soil monoliths. A hypothesis about an increase in the range of plastic behavior of soils and a decrease in the strength of soil structure with an increase in porosity was suggested.