Effects of season and body condition on appetite, body mass and body composition in ad libitum fed pony mares

Changes in appetite, body mass (BM), body condition score (BCS), direct (ultrasonographic) and indirect (deuterium oxide dilution technique) measures of body fat were monitored in Welsh Mountain pony mares (n = 11, 5–19 years of age) offered ad libitum access to a complete diet (gross energy 16.9 ± 0.07 MJ/kg dry matter) for 12 weeks during summer (n = 6; 246 ± 20 kg) and winter (n = 5; 219 ± 21 kg). At the outset, each group comprised two thin (BCS 1–3/9), moderate (BCS 4–6/9) and obese (BCS 7–9/9) animals.For ponies that were non-obese at the outset, BM was gained more rapidly (P = 0.001) in summer (0.8 ± 0.1 kg/day) than winter (0.6 ± 0.0 kg/day). This was associated with a seasonal increase in dry matter intake (DMI) which became maximal (summer, 4.6 ± 0.3% BM as DMI/day; winter, 3.5 ± 0.1% BM as DMI/day) during the second month. The appetite of the obese ponies was half that reported for non-obese animals in the summer and BM remained constant irrespective of season.Body ‘fatness’ increased progressively for non-obese but not obese ponies. Body fat content was exponentially associated with increasing BCS but BCSs >6 were not useful indicators of actual body fat. Endogenous circannual mechanisms to suppress winter weight gain were insufficient to prevent the development of obesity in ad libitum fed ponies.     

Body growth of captive eland Taurotragus oryx in Natal

Post-natal growth in live mass and in body dimensions with age is described in a captive herd of eland Taurotragus oryx, using the von Bertalanffy growth equations. Asymptotic live mass of males (575 kg) was 45% higher than the value for females (316 kg). Asymptotic body dimensions were also higher in males, girth by 27%, height at withers by 13% and body length by 9%, these data illustrating the sexually dimorphic nature of growth in eland. In winter, body growth rates were significantly lower than in the summer, but these variations are not apparent from theoretical von Bertalanffy growth curves. A combination of linear measurements is described for the prediction of live mass.     

Factors affecting milk yield,milk fat,bacterial score,and bulk tank somatic cell count of dairy farms in the central region of Thailand

A study was conducted to determine the effects of season, farm location, and farm size on farm milk yield (FMY), average milk yield per cow (AYC), milk fat, bacterial score, and bulk tank somatic cell count (BTSCC) of dairy farms in the central region of Thailand. Farms were located in the districts of Kaeng Khoi, Muaklek, Pak Chong, and Wang Muang. Collection of data was at the farm level; individual animal records were unavailable. A total of 967,110 daily farm milk yield, 58,575 milk fat and bacterial score, and 24,109 BTSCC records from 1,034 farms were collected from July of 2003 to June of 2006. There were three seasons: rainy, summer and winter. Farms were categorized into small, medium, and large according to the number of cows milked per day. Results showed that FMY and AYC were higher (p <0.05) in winter and lower in the summer and rainy seasons. In addition, the majority of small size farms had higher (p < 0.05) AYC and milk fat values, and lower bacterial score and BTSCC values than medium and large size farms.     

Effect of summer heat environment on body temperature,estrous cycles and blood antioxidant levels in Japanese Black cow

This study investigated the effect of summer heat environment on estrous cycles and blood antioxidant levels in Japanese Black cows. A total of 13 non‐lactating Japanese Black cows (summer: 9, winter: 4) were examined. Body temperature was measured rectally and intravaginally using a thermometer and data logger, respectively. Estrous behavior was monitored using a radiotelemetric pedometer that recorded walking activity. Rectal temperatures were higher during summer than winter (P < 0.001). There was an acute increase in vaginal temperature at the onset of estrus during winter but such an increase was not observed during summer. Walking activity during estrus decreased dramatically in the summer compared to the winter. Duration of estrous cycle was longer in summer (23.4 days, P < 0.05) than winter (21.5 days), and the subsequent rise in progesterone concentrations following estrus tended to be delayed in summer. The level of thiobarbituric acid reactive substances (TBARS) in peripheral blood cells was higher during summer (P < 0.05), while the levels of superoixde dismutase (SOD), glutathione peroxidase (GPx) and glutathione were lower (P < 0.05). These results indicate that high ambient temperature during summer increases both body temperature and oxidative stress, and also reduces signs of estrus in Japanese Black cows.     

ORIGINAL ARTICLE: Echinacea purpurea and Allium sativum as immunostimulants in fish culture using Nile tilapia (Oreochromis niloticus)

The study was conducted to evaluate the efficiency of echnicacea (E) and garlic (G) supplemented diets as immunostimulant for tilapia (Oreochromis niloticus). Seven treatments were designed including a control (C). Fish were fed on 35% protein diet at a rate of 3% body weight per day. Echinacea (1.0 ppt) and garlic (3%) were incorporated in the feed, which was administered for periods of 1, 2 and 3 months (summer season), followed by basal diet for 4 more months (winter season). Neutrophil adherence and haematocrit values increased in both supplemented groups with prolonging period of application. The neutrophils adherence was significantly increased in all treatments except group administered echinacea for 1 month. The lymphocytic counts were significantly (p < 0.004) elevated that resulted in a significant increase in the total leucocytic count in groups administered echinacea for 1 and 2 months when compared with the control and/or other treatments. The gain in the body weight and specific growth rate was significantly increased in all supplemented groups (p < 0.004) during summer, but remained without any significant increase after winter. The survival rate was significantly high (>85%) in all the supplemented groups. The percentage of protection, after challenge infection using pathogenic Aeromonas hydrophila was the highest in groups supplemented with echinacea and garlic for 3 months after summer and winter seasons. It could be concluded that echinacea and garlic improve the gain in body weight, survival rate and resistance against challenge infection. Both compounds showed extended effects after withdrawal and improved resistance to cold stress during the winter season. However, a full commercial cost benefit analysis is necessary before recommending their application in aquaculture.     

Effects of temperature and hypoxic stress on the oxygen consumption rates of the mudsucker fish Labeo capensis (Smith)

The specific oxygen consumption rate (VO ₂ IMb) of Labeo capensis, the freshwater mudsucker, was determined for small and large fish at winter (8°C) and summer (23°C) temperatures. VO JMb was also determined during hypoxic conditions of the experimental water. It was found that VO JMb does not differ substantially compared with other active fish. The VO 4Mb for a fish with a body mass of 250 g is 0,053 ml 0 ₂ per gram wet body mass per hour for fish acclimated at 8°C and 0,127 ml 0 ₂ per gram per hour for fish acclimated at 23°C. The calculated mass exponent, b, is 0,71 at 23°C and 0,68 at 8°C. Hypoxic conditions are relatively well tolerated especially by 23°C- adapted fish.     

Population dynamics of Lemniscomys rosaiia (Muridae: Rodentia) in a Swaziland grassland: effects of food and fire

The effects of food supplementation on a population of Lemniscomys rosalia were studied experimentally in a grassland habitat in Swaziland. Food was added bi-weekly to two 1-ha grids, while a single 1-ha grid served as the control. Rodent traps were set monthly over a 12 month period. Food supplementation may have affected the density of L. rosalia, but did not affect any other features of the population. Breeding commenced in September and ended in April (males) and May (females). Recruitment of juveniles occurred between January and March, Adult mean body mass increased from a low in winter (June/July) to a high in late summer (February). The drop in mean body mass in autumn was due to the disappearance of heavy adults and entry of the lighter subadults into the population. Mean survival of L. rosalia was low; 81% of all captured individuals disappeared within four months. An unscheduled fire burnt part of the study area and hence allowed an assessment of the effect of fire on L. rosalia. The fire did not cause undue mortality of L. rosalia, however, burnt areas were avoided for three months until grass cover had sufficiently increased.     

Seasonal changes in digestibility, passage rate and rumen fermentation of alfalfa hay in sika deer (Cervus nippon) under restricted feeding

To investigate seasonal variations in the digestive functions of sika deer, five female sika deer were provided with an amount of alfalfa hay cubes equivalent to voluntary food intake during winter. We measured the rate at which the food passed through the digestive tract, digestibility and rumen fermentation during the summer (August), autumn (November), winter (February) and spring (May). Total mean retention time in the digestive tract during summer and autumn was numerically longer than that in winter and spring, but the difference did not reach significance. Organic matter and fiber were less digestible in summer and autumn than in winter and spring (P < 0.05), whereas the digestibility of the dry matter tended to vary with the seasons (P < 0.1). Ruminal pH values seasonally changed (P < 0.01), and were the lowest in autumn. The concentration of ruminal ammonia‐nitrogen differed significantly among the seasons (P < 0.05), increasing in winter and decreasing during spring and summer. The numbers of protozoa changed significantly among the seasons (P < 0.05), being higher in autumn than in winter and spring, and intermediate in summer. The concentration of total volatile fatty acids was not seasonally affected, but the molar percentages of propionic acid and butyric acid significantly changed according to season (P < 0.05 and P < 0.01, respectively), and the ratio of acetic to propionic acid tended to change with the seasons (P < 0.1). The results of this study suggested that the digestive functions in sika deer, fed a commercial diet at a restricted level, differed notably among the seasons and these variations might partially be due to environmental effects.     

The Effect of Season on Muscle Growth,Fat Deposition,Travel Patterns,and Hoof Growth of Domestic Young Horses

Our objective was to determine the influence of season (winter, spring, summer, and fall) on travel patterns, hoof growth, and longissimus dorsi muscle (LM) height and fat thickness between 13th and 14th ribs in 16 horses aged <4 years (eight males and eight females) of Morgan, Quarter Horse, and Moriesian breeds. Real-time ultrasound images of LM height and fat thickness as well as measures of hoof growth were obtained at the end of each season. Global positioning system tracking was conducted for four randomly selected days and one storm day in each season. Data were analyzed using a linear mixed model procedure in SAS. Season influenced fat deposition (P < .01) with the greatest increase in fall (P < .05) but had minimal effect on muscle growth. Hoof growth was greatest in summer and least in winter (P < .01). The average distance traveled was greater in spring and summer than in fall and winter (P < .01). The horses moved for less time and traveled less distance on storm days (P < .05) compared with nonstorm days. Young horses also traveled less on storm days, which indicates that it may be especially important to provide shelter for them. It was concluded that season influenced fat deposition, distance traveled, and hoof growth of domestic young horses. A better understanding of these factors could help equine professionals manage young horses more efficiently to benefit the horses’ physical well-being.     

Genome‐wide association studies revealed candidate genes for tail fat deposition and body size in the Hulun Buir sheep

Fat‐tailed sheep have a unique characteristic of depositing fat in their tails. In the present study, we conducted genome‐wide association studies (GWAS) on traits related to tail fat deposition and body size in the Hulun Buir sheep. A total number of 300 individuals belonging to two fat‐tailed lines of the Hulun Buir sheep breed genotyped with the Ovine Infinium HD SNP BeadChip were included in the current study. Two mixed models, one for continuous and one for binary phenotypic traits, were employed to analyse ten traits, that is, body length (BL), body height (BH), chest girth (CG), tail length (TL), tail width (TW), tail circumference (TC), carcass weight (CW), tail fat weight (TF), ratio of CW to TF (RCT) and tail type (TT). We identified 7, 6, 7, 2, 10 and 1 SNPs significantly associated with traits TF, CW, RCT, TW, TT and CG, respectively. Their associated genomic regions harboured 42 positional candidate genes. Out of them, 13 candidate genes including SMURF2, FBF1, DTNBP1, SETD7 and RBM11 have been associated with fat metabolism in sheep. The RBM11 gene has already been identified in a previous study on signatures of selection in this specific sheep population. Two more genes, that is, SMARCA5 and GAB1 were associated with body size in sheep. The present study has identified candidate genes that might be implicated in tail fat deposition and body size in sheep.     

Male and female cats have different regional body compositions and energy requirements for weight loss and weight maintenance

Body composition and energy requirements are different between males and females in several species, and both interfere with weight loss. The aim of this study was to compare the total and regional body composition and energy requirements in obese male (n = 8) and female (n = 8) cats, during weight loss and weight maintenance over 17 subsequent weeks after regimen. The total and regional (thoracic and pelvic limbs, and trunk) body composition was assessed by dual‐energy X‐ray absorptiometry (DXA). Females exhibited a higher fat mass (FM) than males (p < 0.05), and the trunk was the site with greater fat accumulation regardless of gender. A 23.0 ± 2.8% reduction in body weight was followed by a 50.3 ± 9.4% and a 37.0 ± 8.9% reduction in fat in the trunk region in males and females respectively. Lean mass (LM) mobilization was also increased in the trunk (p < 0.05), and the loss of LM was associated with a reduction in bone mass. The energy intake to achieve the same rate of weight loss was 12.9 ± 3.4% higher in males (p < 0.05). The cats exhibited a gradual increase in energy requirements to maintain their body weight after weight loss (p < 0.05). It was concluded that obese cats mainly accumulate fat in the trunk. The reduction in lean mass after the regimen also occurred in the trunk, with no modifications in the skeletal muscle mass of the limbs. Neutered male cats have higher energy requirements than neutered females, and gender should be considered during obesity management in cats.     

Effects of clay on fat necrosis and carcass characteristics in Japanese Black steers

Twenty 10‐month‐old Japanese Black steers were used to evaluate the effects of clay on fat necrosis and carcass characteristics. Ten steers (Clay group) were fed the clay (50 g/day) during 10–30 months of age. The other 10 steers (Control group) were not fed it. There was no significant difference in body weight or average daily gain between the two groups (P > 0.05). The occurrence of fat necrotic mass in the Clay group (30%) was lower (P < 0.05) than that in the Control group (90%) at slaughter. The size of necrotic masses in the Clay group was smaller (P < 0.05) than that in the Control group. There was no significant difference in the marbling score, beef color, Longissimus muscle area or subcutaneous fat thickness between the two groups. These results suggest that the clay prevented the occurrence of fat necrosis and did not affect the carcass characteristics in Japanese Black steers.     

Pyrrolizidine alkaloidosis of cattle associated with Senecio lautu

Summary Serious incidents of pyrrolizidine alkaloidosis of cattle in 10 herds exposed to the Australian native plant, Senecio lautus (Asteraceae), were seen in central Queensland during 1988–1992. The deaths of 226 cattle were recorded. A mean of 8% of cattle died in affected groups (range 2 to 58%). Sickness and deaths usually occurred some months after access to S lautus. Typically, affected cattle lost body condition to the point of emaciation before dying and had persistent diarrhoea. Some animals developed abnormal behaviour and died after a shorter illness. Liver specimens from affected cattle in all herds contained lesions consistent with pyrrolizidine alkaloidosis. Thin layer chromatography of extracts of blood and liver samples from cattle from 5 herds detected pyrrolic metabolites. The identity of these was confirmed by mass spectroscopy on samples from one herd. Unseasonal autumn and winter rain after a dry summer appeared to favour growth of S lautus at the expense of other pasture species. A subsequent dry period promoted consumption of S lautus and was followed by a cluster of poisoning incidents.     

Seasonal differences in rumen bacterial flora of wild Hokkaido sika deer and partial characterization of an unknown bacterial group possibly involved in fiber digestion in winter

Rumen digesta was obtained from wild Hokkaido sika deer to compare bacterial flora between summer and winter. Bacterial flora was characterized with molecular‐based approaches and enrichment cultivation. Bacteroidetes was shown as a major phylum followed by Firmicutes, with similar proportions in both seasons. However, two phylogenetically unique groups in Bacteroidetes were found in each season: unknown group A in winter and unknown group B in summer. The ruminal abundance of unknown group A was the highest followed by Ruminococcus flavefaciens in winter. Moreover, the abundance of these two was higher in winter than in summer. In contrast, the abundance of unknown group B was higher in summer than in winter. In addition, this group showed the highest abundance in summer among the bacteria quantified. Unknown group A was successfully enriched by cultivating with oak bark and sterilized rumen fluid, particularly that from deer. Bacteria of this group were distributed in association with the solid rather than the liquid rumen fraction, and were detected as small cocci. Accordingly, unknown group A is assumed to be involved in degradation of fibrous materials. These results suggest that wild Hokkaido sika deer develop a rumen bacterial flora in response to changes in dietary conditions.     

Age determination and age structure of a striped fieldmouse,Rhabdomys pumilio,population from the Cape Flats

The validity of predicting the age of Rhabdomys pumilio by age-related changes in tooth appearance, dimensions and position, in body mass and head-body length is tested against 47 known-age mice. Body mass can be used to determine the age of individuals of less than 7 weeks old (≤ 26 g). Thereafter, the only reliable technique of age determination Involves a visual evaluation of the degree of molar tooth wear. Five wear classes are described and used to assess the age of 780 R. pumilio collected during a five-year period. The annual cycles of population age structure and size were dependent on seasonal recruitment and were Influenced by the duration of the peak breeding period. The growth rate, especially of females, is reduced in winter, and maturity appears to be delayed until spring, when breeding commences.     

冬夏两季环境温度对藏猪生理特征及相关基因表达的影响

试验旨在探讨藏猪低温适应性相关指标,为研究藏猪种质特征提供理论依据,以期更好地开发和利用地方品种资源。在甘肃榆中县某藏猪养殖场随机选择哺乳期间体况良好的20日龄仔猪（分别于冬季环境温度-16 ℃和夏季环境温度30 ℃时选择藏猪各4只）,对其体尺指标、呼吸频率、背部脂肪厚度、皮肤厚度、血液生理生化指标进行测量。HE染色观察腹股沟、肩胛、背部的脂肪细胞形态,并进一步采用实时荧光定量PCR技术检测产热相关基因在不同部位脂肪组织间的相对表达情况。结果显示,冬季低温组藏猪的呼吸频率极显著低于夏季高温组藏猪（P<0.01）;背部脂肪厚度和颈部、背部、臀部皮肤厚度均极显著高于高温组藏猪（P<0.01）;低温组藏猪的血小板数目（PLT）、血小板积压（PCT）、高密度脂蛋白胆固醇（HDL-C）、葡萄糖（GLU）、甘油三酯（TG）含量显著高于高温组藏猪（P<0.05）;平均血小板体积（MPV）和血小板分布宽度（PDW）显著低于高温组藏猪（P<0.05）。HE染色结果显示,低温组藏猪腹股沟和肩胛处的脂肪细胞大小不等,存在多腔脂滴形态;同时发现低温组藏猪腹股沟和肩胛部解偶联蛋白3（uncouple protein3,UCP3）、过氧化物酶体增殖物激活受体-γ共激活因子-1α（peroxisome proliferator-activated receptorγcoactivator-1α,PGC-1α）和Ⅱ型脱碘酶（type 2 iodothyronine deiodinase,Dio2）基因mRNA的相对表达量显著高于高温组藏猪（P<0.05）。综上所述,环境温度对藏猪的生理指标和脂肪代谢等方面有较大影响,并且低温刺激白色脂肪褐变是藏猪御寒的重要因素之一。     

Effect of roasted or raw peanut kernels on lactation performance and milk composition of swine

A lactation trial involving 105 sows was conducted to determine the effect of 12% roasted or raw, ground, whole, shelled peanuts on sow weight change during lactation, feed intake, piglet and litter weight gain, milk composition, and days to return to postweaning estrus. The trial was conducted using three sow groups during two farrowing seasons, summer (July to September) and winter (December to February). Diets were based on corn plus soybean meal. Diets contained either 5% animal fat or equivalent added fat from 12% roasted or raw, ground, shelled peanuts. The replacement of animal fat by roasted or raw peanuts had no effect (P greater than .20) on sow weight change, average daily feed intake during lactation or days to estrus postweaning, or on piglet weight gain or survival. Milk composition (percentage fat and protein) was not altered (P greater than .20) by source of fat in the summer; however, in the winter, sows fed roasted peanuts had higher (P less than .05) milk fat and protein percentage at 3 d postfarrowing than other treatment groups. At d 7, sows fed 12% roasted or raw peanuts had higher (P less than .05) milk protein than sows fed 5% animal fat. Sows farrowing in the summer had greater (P less than .01) weight loss and consumed less (P less than .05) feed during lactation than sows farrowing in the winter. Sows farrowed in the summer had larger (P less than .05) litters at birth and 14 d postfarrowing and greater (P less than .10) piglet and litter weight gain postfarrowing than those farrowed in the winter.(ABSTRACT TRUNCATED AT 250 WORDS)     

Exploring relationships between body condition score,body fat,activity level and inflammatory biomarkers

Obesity is associated with inflammatory disorders in humans, including degenerative joint disease. While obesity is endemic in horses, its relationship to equine degenerative joint disease has not been explored. The current study sought to describe relationships between: body weight (BW), body condition score (BCS), lameness grade (AAEP), total body fat mass (kg; FM) and fat per cent (FP) [multifrequency bioelectrical impedance analysis (mfBIA)], age, gender, activity level (AL), synovial fluid (SF) and plasma (PL) PGE₂ and glycosaminoglycan (GAG) in horses. During this field investigation, the BCS (of nine) of 54 horses at multiple farms in southern Ontario, Canada, was determined. Horses were categorized as thin (BCS=3/9; n = 6), moderate (BCS=4 or 5/9; n = 18), overweight (BCS=6 or 7/9; n = 19) or obese (BCS=8 or 9/9; n = 11). Total fat mass (kg) and body fat% was measured using mfBIA, lameness was assessed (AAEP lameness scale) and synovial fluid was collected via aseptic arthrocentesis from the left intercarpal joint for assessment of inflammatory biomarkers (PGE₂, GAG). Means were compared with a one‐way ANOVA; correlation coefficients were calculated using a Spearman Rank Order Correlation to reveal correlations between variables. BCS was positively correlated with BW, FM, FP, AL and PL‐PGE₂. BW was also significantly positively correlated with PL‐PGE₂. It is concluded that BCS is significantly correlated with PL‐PGE₂, due in part to the combined effect of AL and body condition. Net inflammatory effects of body fat on risk for joint disease require further study.     

The Effect of Season and Vaccination for Glässer's Disease and Post‐weaning Colibacillosis in an Outdoor Pig Unit Endemically Infected with Virulent Strain of Haemophilus Parasuis Serotype 5 and Pathogenic Escherichia coli

The objective of this field trial was to determine if vaccination against Haemophilus parasuis serovar 5 (HPS 5) and pathogenic serotypes of Escherichia coli would improve nursery pig performance in an outdoor unit in different seasons. The unit was concurrently infected with HPS 5 and with different serotypes of E. coli. All piglets were born to HPS 5 vaccinated sows. The trial was carried out in four (two summer and two winter) groups. Group 1 (E. coli and HPS vaccinated, summer season) (n = 362): Piglets were vaccinated pre‐weaning with inactivated E. coli‐VT2e‐toxin and post‐weaning against HPS 5. Group 2 (non‐vaccinated, summer season) (n = 349): Piglets were not vaccinated. Group 3 (E. coli and HPS vaccinated, winter season) (n = 358): The animals were analogously treated as Group 1. Group 4 (non‐vaccinated, winter season) (n = 353): Piglets were not vaccinated. The following parameters were evaluated: A: average daily nursery weight gain (ADG), B: nursery mortality, C: feed efficiency (FE). No significant weight differences were detected within the vaccinated and non‐vaccinated summer or winter raised groups of weaners. Summer raised weaners were significantly (P<0.05) heavier from day 35 on than winter raised animals. ADG and FE of summer raised pigs were significantly better (weeks 1–3 P<0.05; fourth week post‐weaning P<0.01) during the nursery period than that of the winter raised groups. Winter raised vaccinated weaners showed during the last week of nursing significantly (P<0.05) better daily gain and feed efficiency compared with the non‐vaccinated winter raised animals. Non‐significant ADG and FE differences were detectable between the summer raised vaccinated or non‐vaccinated groups of pig. Winter raised non‐vaccinated animals suffered significantly (P<0.05) higher nursery mortality (10.63%) compared to the winter raised vaccinated animals. Implication: In cases of concurrent infections with HPS 5 and with different serotypes of E. coli, especially during winter season, vaccination against both diseases is suggested.     

慢性冷热应激对放牧西门塔尔牛抗氧化功能及HPA轴、SAM轴激素分泌的影响

为研究慢性冷热应激对放牧条件下西门塔尔牛生理指标、抗氧化功能及下丘脑-垂体-肾上腺皮质轴(HPA)、交感-肾上腺-髓质轴(SAM)激素分泌的影响,选取内蒙古锡林郭勒盟苏尼特左旗某家庭牧场体质量相近、体况健康的西门塔尔牛20头,试验期间测定试验牛生理指标,于试验期最后一天清晨进行颈静脉采血并制备血清,采用ELISA方法检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、促肾上腺皮质激素(ACTH)、糖皮质激素(GC)、肾上腺素(EPI)及去甲肾上腺素(NE)的含量。结果表明,冷热应激对西门塔尔牛产生了一定的应激:冷应激状态下(冬季)西门塔尔牛血清中ACTH、GC含量显著高于热应激状态(夏季)和非应激状态(春季)(P<0.05),夏季和冬季西门塔尔牛血清中NE、EPI含量均显著高于春季(P<0.05);夏季和冬季西门塔尔牛血清中MDA含量显著高于春季(P<0.05),春季和夏季T-AOC含量显著高于冬季(P<0.05),GSH-Px含量在3个季节之间差异不显著(P>0.05)。综上所述,冷热应激降低了西门塔尔牛的抗氧化能力,可能对西门塔尔牛机体造成了一定的损伤;冷热应激激活了西门塔尔牛的HPA轴和SAM轴,进而提高机体对冷热应激的适应能力。