Growth of the whole root system for a plant crop of sugarcane under rainfed and irrigated environments in Brazil

Sugarcane crops are managed over 8 million hectares in Brazil and future extensions might occur on less favorable lands where irrigation would be necessary to increase and stabilize yields. Root growth was studied by sequential soil coring under rainfed and irrigated conditions for one cultivar widely planted in Brazil. Root length densities (RLD) were measured 34, 49, 125, 179, 241 and 322 days after planting (DAP) down to a depth of 1 m. At the harvest (332 DAP), root intersects (a proxy for RLD) were counted on two vertical trench walls in each water supply regime, down to a depth of 6.0 m. The highest RLD in deep layers (below a depth of 0.6 m) were observed in the rainfed crop from 125 DAP onwards. By contrast, the highest RLD in the upper layers during dry periods were found in the irrigated crop. The maximum depth reached by roots at the harvest was little affected by irrigation: 4.70 m and 4.25 m in the rainfed and irrigated crop, respectively. About 50% of root intersects were observed below the depth of 1 m in the two water supply regimes. This pattern suggested a strong genetic control of root growth in deep soil layers. The total amount of root intersects 332 DAP was 49% higher in the rainfed crop than in the irrigated crop, and root distribution was more homogeneous. Mean root front velocity was about 0.5 cm day⁻¹ the first 4 months after planting and increased thereafter up to the end of the harvest (1.86 cm day⁻¹ and 1.75 cm day⁻¹ on average in the rainfed and the irrigated crops, respectively). Our study pointed out the necessity to take into account the development of sugarcane roots in deep soil layers to improve our understanding of net primary production control by water availability.     

Association between aflatoxin contamination and drought tolerance traits in peanut

The current study investigates the association between drought tolerance traits and aflatoxin contamination in peanut grown under long-term drought. Two field experiments were conducted at Khon Kaen University, Thailand using a split–split plot design with three drought stress levels as main plots, 11 genotypes as sub-plots, and two soil inoculations of Aspergillus flavus treatments as sub-sub-plots. The effects of temperature, soil moisture and A. flavus population on kernel colonization and aflatoxin contamination, and drought tolerance traits viz. specific leaf area (SLA) and root length density (RLD) were measured. The results demonstrated that elevated soil temperatures and reduced soil moisture, favored aflatoxin production. Drought in combination with higher levels of A. flavus inoculum load in the soil resulted in an increase in the fungal populations in the soil which in turn resulted in increased kernel colonization and subsequent aflatoxin contamination. A combination of SLA and RLD, and kernel colonization had a significant influence on aflatoxin contamination under drought conditions in both seasons (r = 0.73** and 0.76**). The results revealed that drought tolerance traits (SLA and RLD) could be contributing to resistance to aflatoxin contamination suggesting that a combination of SLA, RLD and kernel colonization could be used as selection criteria in selecting parents for aflatoxin resistance.     

Root Growth and Water Extraction Response of Doubled-Haploid Rice Lines to Drought and Rewatering during the Vegetative Stage

Abstract

Doubled haploid lines (DHLs) of rice (Oryza sativa L.) were used to examine responses to drought and rewatering in controlled rainfed lowland conditions, in order to determine whether confounding by unrelated traits would be less than has been reported previously for contrasting cultivars that differ in genetic background. IR62266 and four DHLs derived from the cross between IR62266 and CT9993 (DHL-32, -51, -54 and -79) were grown in pot experiments in the greenhouse during the 2000 dry and wet seasons at IRRI, Los Baños, Philippines. There were two water regimes (well-watered and drought). Estimated water extraction obtained by time domain reflectometry (TDR) was similar to cumulative transpiration estimated from pot weighing for each genotype. Genotypic variation was observed in root traits and water extraction, with extraction slower in DHL-32 and faster in DHL-79, especially in deeper soil layers. An upper bound relationship between water extraction from a soil layer and root length density (RLD) in that layer was readily apparent over DHLs and soil depths, suggesting a critical value of RLD for water extraction of 0.30 cm cm^?3 in these conditions. Because soils in the field would not be as homogenous as the puddled soils used in these greenhouse experiments, this critical RLD for water extraction from a soil layer is a reference for ideal conditions, and requires careful validation in the field. Use of DHLs permitted comparisons with reduced confounding by genetic background, with consequent improvements in precision.     

Mapping quantitative trait loci associated with root growth in upland rice (Oryza sativa L.) exposed to soil water-deficit in fields with contrasting soil properties

A mapping population of 114 lines from Bala × Azucena was grown under drought stress at two field sites with contrasting soil physical properties. Drought was imposed between 35 and 65 days after sowing (DAS) and root density at 35 cm depth was measured 70 DAS. Leaf rolling, leaf drying and relative water content were recorded as indicators of drought avoidance. Root density correlated with indicators of drought avoidance. Two significant and two putative quantitative trait loci (QTLs) for root density and 28 QTLs for drought avoidance were identified. Most QTLs did not agree between sites. There was also reasonable agreement between leaf-drying QTLs and previously reported root-growth QTLs detected under controlled conditions (in contrast to a previous screen on soil with a higher penetration resistance). These data also reveal QTL × environment interaction, which will need to be understood more clearly if progress towards breeding for drought resistance via alterations of root morphology is to be achieved.     

Radiation use efficiency,N accumulation and biomass production of high-yielding rice in aerobic culture

The concept of aerobic culture is to save water resource while maintaining high productivity in irrigated rice ecosystem. This study compared nitrogen (N) accumulation and radiation use efficiency (RUE) in the biomass production of rice crops in aerobic and flooded cultures. The total water input was 800–1300 mm and 1500–3500 mm in aerobic culture and flooded culture, respectively, and four high-yielding rice cultivars were grown with a high rate of N application (180 kg N ha⁻¹) at two sites (Tokyo and Osaka) in Japan in 2007 and 2008. The aboveground biomass and N accumulation at maturity were significantly higher in aerobic culture (17.2–18.5 t ha⁻¹ and 194–233  kg N ha⁻¹, respectively) than in flooded culture (14.7–15.8 t ha⁻¹ and 142–173 kg N ha⁻¹) except in Tokyo in 2007, where the surface soil moisture content frequently declined. The crop maintained higher N uptake in aerobic culture than in flooded culture, because in aerobic culture there was a higher N accumulation rate in the reproductive stage. RUE in aerobic culture was comparable to, or higher than, that in flooded culture (1.27–1.50 g MJ⁻¹ vs. 1.20–1.37 g MJ⁻¹), except in Tokyo in 2007 (1.30 g MJ⁻¹ vs. 1.37 g MJ⁻¹). These results suggest that higher biomass production in aerobic culture was attributable to greater N accumulation, leading to higher N concentration (N%) than in flooded culture. Cultivar differences in response to water regimes were thought to reflect differences in mainly (1) early vigor and RUE under temporary declines in soil moisture in aerobic culture and (2) the ability to maintain high N% in flooded culture.     

Root lodging in sunflower. Variations in anchorage strength across genotypes,soil types,crop population densities and crop developmental stages

Root lodging is an important adversity affecting sunflower (Helianthus annuus L.) production in Argentina under current husbandry practices, and may limit progress towards the achievement of higher yields via increased plant population density. Although there are perceptions that lodging susceptibility varies across developmental stages, crop population densities, genotypes and soil types, these perceptions have not been tested for sunflower using a standardized experimental protocol. This study aimed at: (1) identifying the sources of the variation in root lodging susceptibility in response to variations in crop population density in two genotypes of reputedly different susceptibility; (2) detecting the crop developmental stages most susceptible to root lodging; and (3) examining the relationships between root failure moment, root plate diameter and soil shear strength. We mechanically induced lodging at three developmental stages in plants rooted in pre-wetted plots. The crops were grown at 5.6 plants m⁻² over 3 years on either Typic Argiudoll or Typic Hapludoll soils and at 3, 5.6, 10 and 16 plants m⁻² on a Typic Argiudoll. The force needed to induce root lodging (root failure moment) and root plate diameter varied across genotypes, plant densities and developmental stages. Root failure moment and root plate diameters were greater (p < 0.05 for both variables) in the resistant hybrid across the three development stages and almost all crop population densities. For both hybrids, the most susceptible development stage was R2, and root failure moment and root plate diameter diminished (p < 0.05) as crop population density increased. Although root failure moment did not differ between soil types, root plate diameter was greater (p < 0.0001) in the coarser soil. The relationship between root failure moment and the product of root plate diameter cubed by soil shear strength (a measure of plant anchorage strength) for both hybrids, both soil types, and all crop population densities could be described by a single linear relationship (y = 0.2382x; R² = 0.812; p < 0.025).     

Mixing groundnut residues and rice straw to improve rice yield and N use efficiency

Groundnut as a pre-rice crop is usually harvested 1–2 months before rice transplanting. During this lag phase much of N in groundnut residues could be lost due to rapid N mineralization. Mixing of abundantly available rice straw with groundnut residues may be a means for reducing N and improve subsequent crop yields. The objectives of this experiment were to investigate the effect of mixing groundnut residues and rice straw in different proportions on (a) growth and yield of succeeding rice, (b) groundnut residue N use efficiency and (c) N lost (¹⁵N balance) from the plant–soil system and fate of residue N in soil fractions. The experiment consisted of six treatments: (i) control (no residues), (ii) NPK (at recommended rate, 38 kg N ha⁻¹), (iii) groundnut residues 5 Mg ha⁻¹ (120 kg N ha⁻¹), (iv) rice straw 5 Mg ha⁻¹ (25 kg N ha⁻¹), (v) 1:0.5 mixed (groundnut residues 5 Mg: rice straw 2.5 Mg ha⁻¹), and (vi) 1:1 mixed (groundnut residues 5 Mg: rice straw 5 Mg ha⁻¹). After rice transplanting, samples of the lowland rice cultivar KDML 105 were periodically collected to determine growth and nutrient uptake. At final harvest, dry weight, nutrient contents and ¹⁵N recovery of labeled groundnut residues were evaluated.     

Higher crop performance of rice hybrids than of elite inbreds in the tropics: 2. Does sink regulation,rather than sink size,play a major role?

Higher harvest index, widely reported as a main trait supporting major improvements in rice yield potential, also supports the higher performance of hybrids over inbreds. Although higher sink size is generally claimed for being the driver of higher grain filling in hybrid rice, it is relevant to question whether efficient sink regulation, that is timing and magnitude of the successive sink activities over crop growth from early stage, plays a role in supporting higher harvest index and is a key feature ensuring higher performance of hybrid rice. The partitioning coefficient (PC), as the increase in dry matter of one single organ over that of shoot, was calculated over short and successive periods to quantify the dynamics of dry matter partitioning. Four hybrid and four inbred genotypes of similar crop duration were grown under the same cropping management on the IRRI farm during a wet and a dry seasons. While PC to blade and sheath was similar for both plant types in both seasons at the early stage, PC to culm of hybrids was higher than that of inbreds, and PC to blade was lower, in the late vegetative and early reproductive phases. During the late reproductive phase, PC to panicle of hybrids was higher than that of inbreds, and PC to culm was lower whereas culm elongation was similar for both plant types. During grain filling, PC to culm was significantly more negative with hybrids which indicated stronger remobilization with hybrids. Specific culm length before anthesis, as low as 40 cm g⁻¹, accounted for storage ability, and at maturity, as high as 130 cm g⁻¹, for remobilization ability. Sink strength index, as a better indicator of dry matter partitioning efficiency than harvest index, and unfilled grain size, as an indicator of assimilate wastage, were suggested as key indices to account for the better sink regulation in hybrid rice and to improve screening protocols for increasing yield potential and tolerance to lodging.     

Variability of root length density and its contributions to seed yield in chickpea (Cicer arietinum L.) under terminal drought stress

The importance of root systems in acquiring water has long been recognized as crucial to cope with drought conditions. This investigation was conducted to: (i) evaluate the variability on root length density (RLD) of chickpea in the vegetative growth stage; (ii) estimate the effect of RLD on seed yield under terminal drought conditions; and (iii) set up a procedure to facilitate the screening of chickpea genotypes with large RLD. Twelve diverse chickpea genotypes were grown in tall PVC cylinders with two different soil water treatments in 2000 and 2001, and in field under water deficit conditions during 2000/2001 and 2001/2002. In field trials, the mean RLD at 35 days after sowing showed a significant positive correlation with seed yield in both years. Similarly, the RLD in the 15–30 cm soil depth had significant positive effects to the seed yield in both years. The importance of the root trait was particularly relevant in 2001/2002, a more severe drought year, when the RLD in deeper soil layer, 30–60 cm depth, showed a significant positive relationship with seed yield. Also, the RLD at deeper soil layer, 30–60 cm depth, was higher in 2001/2002 than in 2000/2001, in particular in tolerant genotypes. The PVC cylinder trials were set up to facilitate the screening of chickpea genotypes with large RLD. RLD of plants grown in cylinders with 70% field capacity was correlated with RLD in the field trials (r = 0.731; p = 0.01). This work highlights the importance of roots in coping with terminal drought in chickpea. The cylinder system offers a much easier procedure to screen chickpea genotypes with large RLD.     

Morphological and physiological traits of roots and their relationships with shoot growth in “super” rice

Success in “super” rice breeding has been considered a great progress in rice production in China. This study aimed to test the hypothesis that an improved root system may contribute to better shoot growth and consequently to higher grain yield in “super” rice. Two “super” rice varieties Liangyoupeijiu (an indica hybrid) and Huaidao 9 (a japonica inbred) and two elite check varieties Yangdao 6 (an indica inbred) and Yangfujiang 8 (a japonica inbred) were field-grown at Yangzhou, China in 2006 and 2007. Root and shoot dry weight (DW) was significantly greater in “super” rice varieties than in check ones throughout the growth season in both years, so was the root length density. Root oxidation activity (ROA) and root zeatin (Z) zeatin riboside (ZR) content, in per plant basis, were significantly greater in “super” rice than check varieties before and at heading time. However, both ROA and root Z + ZR content, either in per plant basis or per unit root DW basis, were significantly lower in ‘super’ rice than in check varieties at the mid- and late grain filling stages. Grain yield of the two ‘super’ rice varieties, on average, was 10.2 t ha⁻¹ in 2006 and 11.4 t ha⁻¹ in 2007, and was 13% and 21% higher than that of check varieties, respectively. The high grain yield was mainly due to a larger sink size (total number of spikelets) as a result of a larger panicle. The percentage of filled grains of the two “super” rice varieties, on average, was 72.9% in 2006 and 79.0% in 2007, and was 19.4% and 12.9%, respectively, lower than that of the check varieties. The mean ROA and root Z + ZR content during the grain filling period significantly correlated with the percentage of filled grains. Collectively, the data suggest that an improved root and shoot growth, as showing a larger root and shoot biomass and greater root length density during the whole growing season and higher ROA and root Z + ZR content per plant at early and mid-growth stages, contributes to the large sink size and high grain yield in the “super” rice varieties. The data also suggest the yield of “super” rice varieties can be further increased by an increase in filled grains through enhancing root activity during grain filling.     

Dryland maize yields and water use efficiency in response to tillage/crop stubble and nutrient management practices in China

Rainfed crop production in northern China is constrained by low and variable rainfall. This study explored the effects of tillage/crop residue and nutrient management practices on maize (Zea mays L.) yield, water use efficiency (WUE), and N agronomic use efficiency (NAE) at Shouyang Dryland Farming Experimental Station in northern China during 2003–2008. The experiment was set-up using a split-plot design with 3 tillage/crop residue methods as main treatments: conventional, reduced (till with crop residue incorporated in fall but no-till in spring), and no-till (with crop residue mulching in fall). Sub-treatments were 3 NP fertilizer rates: 105–46, 179–78 and 210–92 kg N and P ha⁻¹. Maize grain yields were greatly influenced by the growing season rainfall and soil water contents at sowing. Mean grain yields over the 6-year period in response to tillage/crop residue treatments were 5604, 5347 and 5185 kg ha⁻¹, under reduced, no-till and conventional tillage, respectively. Grain yields under no-till, were generally higher (+19%) in dry years but lower (−7%) in wet years. Mean WUE was 13.7, 13.6 and 12.6 kg ha⁻¹ mm⁻¹ under reduced, no-till, and conventional tillage, respectively. The no-till treatment had 8–12% more water in the soil profiles than the conventional and reduced tillage treatments at sowing and harvest time. Grain yields, WUE and NAE were highest with the lowest NP fertilizer application rates (at 105 kg N and 46 kg P ha⁻¹) under reduced tillage, while yields and WUE tended to be higher with additional NP fertilizer rates under conventional tillage, however, there was no significant yield increase above the optimum fertilizer rate. In conclusion, maize grain yields, WUE and NAE were highest under reduced tillage at modest NP fertilizer application rates of 105 kg N and 46 kg P ha⁻¹. No-till increased soil water storage by 8–12% and improved WUE compared to conventional tillage, thus showing potentials for drought mitigation and economic use of fertilizers in drought-prone rainfed conditions in northern China.     

Using stress tolerance indicator (STI) to select high grain yield iron-deficiency tolerant wheat genotypes in calcareous soils

Despite large variation among crop genotypes in response to Fe fertilization, there is no reliable indicator for identifying Fe-deficiency tolerant wheat genotypes with high grain yield. The aim of this investigation was to compare the grain yield response of 20 spring and 30 winter bread wheat genotypes to Fe fertilization under field conditions and to select high grain yield Fe-deficiency tolerant genotypes using a stress tolerance indicator (STI). Two individual trials, each one consisting two field plot experiments, were conducted during 2006–2007 and 2007–2008 growing seasons. Spring wheat genotypes (Trial l) and winter wheat genotypes (Trial 2) were planted at two different locations. Two Fe rates (0 and 20 kg Fe ha⁻¹ as Fe-EDTA) were applied. Spring and winter wheat genotypes differed significantly (P < 0.01) in the grain yield both with and without added Fe treatments. Application of Fe fertilizer increased grain yield of spring wheat genotypes by an average of 211 and 551 kg ha⁻¹ in Karaj and Isfahan locations, respectively. By Fe application, the mean grain yield of winter wheat genotypes increased 532 and 798 kg ha⁻¹ in Karaj and Isfahan sites, respectively. Iron efficiency (Fe-EF) significantly differed among wheat genotypes and ranged from 65% to 113% for spring wheat and from 69% to 125% for winter wheat genotypes. No significant correlation was found between Fe-EF and grain yield of spring wheat genotypes under Fe deficient conditions. For winter wheat genotypes grown in Mashhad, Fe-efficiency was not significantly correlated with the grain yield produced without added Fe treatment. The STI was significantly (P < 0.01) varied among spring and winter wheat genotypes. The interaction between location and genotype had no significant effect on the STI. According to these results, the STI should be considered as an effective criterion for screening programs, if a high potential grain yield together with more stable response to Fe fertilization in different environments is desired.     

Possible causes of yield failure in tropical aerobic rice

Aerobic rice is a water-saving rice production system for water-short environments with favorable soils and adapted, potentially high-yielding varieties that are direct dry seeded. Soils remain aerobic but supplementary irrigation is applied as necessary. In the dry season of 2004 and 2005, a water by N experiment was set up at the location “Dapdap” in central Central Luzon, Philippines, to explore water and N management strategies in aerobic rice. The experiment was laid out as a split-plot design on a loamy sand soil with three water treatments (irrigation twice per week, once per week, and once in two weeks with modifications) and 5 N levels (0–200 kg ha⁻¹). Average seasonal soil moisture tension ranged from 9.2 to 20 kPa but yield hardly responded to the treatment combinations and ranged from 0 to 2 t ha⁻¹. In addition to trial-specific parameters, root knot nematodes and micronutrients (2005) were monitored. Galling of roots due to nematodes was assessed through a rating scale of 0–5, with 0 = no galling and 5 = >75% of the root system galled. The degree of galling reached a level of 5 at flowering and harvest in 2004, and 3 at tillering and 4 at harvest in 2005. Results of a plant tissue analysis at mid-tillering for Fe, Mn, and Zn showed on average values above critical levels; individual replicates, however, indicated deficiencies for Mn. In addition to actual field observations, we used simulation modeling (ORYZA2000) as a tool to estimate attainable yield under actual water conditions and N inputs to explore how yield failure set in. Simulation results matched observed values for total above-ground biomass and leaf area index quite well when no N was applied. When high rates of N (200 and 165 kg ha⁻¹) were applied, simulated values matched actual field data only until about the panicle initiation stage; afterward, observed values remained below the simulation. We interpreted this as evidence that growth-limiting factors other than water or N affected the crop from this growth stage on. Observations made in the field on root knot nematodes and micronutrients suggested that these two factors, especially root knot nematodes, may have been major constraints to crop development in this experiment.     

Comparison of Root System Development in Two Rice Cultivars During Stress Recovery from Drought and the Plant Traits for Drought Resistance

Summary

A greenhouse experiment was conducted to compare root system development of two upland rice cultivars, IRAT 13 and Senshou, during recovery from drought stress and to identify the plant traits that confer drought resistance. From 62 days after sowing (62 DAS), drought stress was given for 6 d followed by rewatering for 14 d. Root length density (RLD) and root diameter (thickness) were measured at the end of the stress and rewatering periods. Control plants were well-watered throughout the study. Gultivar IRAT 13 had thicker roots and higher relative RLD (ratio of RLD in drought-stressed plants to that in control plants) than under drought stress, and significantly higher root growth recovery after rewatering cultivar Senshou. Related plant traits such as evapotranspiration (ET), leaf and stem dry weights and weight of senescent leaves (dead leaves) in IRAT 13 were significantly more favorable for drought resistance compared to Senshou.     

Biotic and abiotic causes of yield failure in tropical aerobic rice

Aerobic rice is a new production system for water-short environments. Adapted varieties are usually direct dry seeded and the crop grown under aerobic soil conditions with supplementary irrigation as necessary. Occasionally, yield failures occur which may be related to soil health problems. In the dry season of 2006 and 2007, we conducted a field experiment in the Philippines, to identify the major causes of such yield failure. Four treatments were implemented: (i) Control of direct dry-seeded rice (improved upland variety Apo), (ii) Biocide application, (iii) transplanting into aerobic soil, and (iv) 1 year fallow. Yield in the Biocide treatment was 2 t ha⁻¹ in both years. In all other treatments, yield was 0–0.3 t ha⁻¹. Plants grown in the Biocide treatment showed a reduced degree of galling of roots caused by root-knot nematodes (RKN) and better general root health than the Control treatment. Potentially pathogenic fungi were isolated from root samples (Pythium sp., Fusarium sp., and a Rhizoctonia-like species). Abiotic effects of the Biocide treatment were an increase in KCl-extractable N (initial season 2007) and a decrease in initial soil pH. In the Control treatment, soil pH increased from 6.5 to 8.0 over the two seasons. In 2007, plant tissue analysis indicated Mn deficiency in the Control treatment. Plants that were subjected to foliar micronutrient sprays reacted positively to Fe and Mn sprays in the Control treatment, and Mn spray in the Biocide treatment. We concluded that the Biocide application led to favorable soil conditions by reducing biotic stresses such as RKN and improving nutrient availability. In the other treatments, an interaction of RKN and micronutrient deficiencies with increasing soil pH led to yield failure.     

Soil type,management history and current resource allocation: Three dimensions regulating variability in crop productivity on African smallholder farms

Soil fertility varies markedly within and between African smallholder farms, both as a consequence of inherent factors and differential management. Fields closest to homesteads (homefields) typically receive most nutrients and are more fertile than outlying fields (outfields), with implications for crop production and nutrient use efficiencies. Maize yields following application of 100 kg N ha⁻¹ and different rates and sources of P were assessed on homefields and outfields of smallholder farms in Zimbabwe. Soil organic carbon, available P and exchangeable bases were greater on the homefields than outfields. In each of three experimental seasons, maize yields in homefield control plots were greater than in the outfields of farms on a granitic sandy and a red-clay soil. Application of mineral N significantly increased maize yields on homefields in the first season (2.1–3.0 t ha⁻¹ on the clay soil and 1.0–1.5 t ha⁻¹ on the sandy soil) but the effects of N alone were not significant on the outfields due to other yield-limiting factors. Greatest yields of about 6 t ha⁻¹ were achieved on the clayey homefield with 100 kg N ha⁻¹ and 30 kg P ha⁻¹ applied as single super phosphate (SSP). Manure application gave greater yields (3–4 t ha⁻¹) than SSP (2–3 t ha⁻¹) in the sandy homefield and in the clayey outfield. Maize did not respond significantly to N, dolomitic lime, manure and P on the sandy outfield in the first and second seasons. In the third season, manure application (∼17 t manure ha⁻¹ year⁻¹) on the sandy outfield did result in a significant response in grain yields. Apparent P recovery in the first season was 55–65% when P was applied at 10 kg ha⁻¹ on the clayey homefield (SSP), clayey outfield (SSP and manure) and sandy homefield (manure) with apparent P recovery less than 40% when P was applied at 30 kg ha⁻¹. On the sandy outfield, P recovery was initially poor (<20%), but increased in the successive seasons with manure application. In a second experiment, less than 60 kg N ha⁻¹ was required to attain at least 90% of the maximum yields of 2–3 t ha⁻¹ on the sandy homefield and clayey outfield. N use efficiency varied from >50 kg grain kg⁻¹ N on the infields, to less than 5 kg grain kg⁻¹ N on the sandy outfields. Apparent N recovery efficiency by maize was greatest at small N application rates with P applied. We conclude that blanket fertilizer recommendations are of limited relevance for heterogeneous smallholder farms. Targeted application of mineral fertilizers and manure according to soil type and past management of fields is imperative for improving crop yields and nutrient use efficiencies.     

Effects of transition season management on soil N dynamics and system N balances in rice–wheat rotations of Nepal

In the low-input rice–wheat production systems of Nepal, the N nutrition of both crops is largely based on the supply from soil pools. Declining yield trends call for management interventions aiming at the avoidance of native soil N losses. A field study was conducted at two sites in the lowland and the upper mid-hills of Nepal with contrasting temperature regimes and durations of the dry-to-wet season transition period between the harvest of wheat and the transplanting of lowland rice. Technical options included the return of the straw of the preceding wheat crop, the cultivation of short-cycled crops during the transition season, and combinations of both. Dynamics of soil N_min, nitrate leaching, nitrous oxide emissions, and crop N uptake were studied throughout the year between 2004 and 2005 and partial N balances of the cropping systems were established. In the traditional system (bare fallow between wheat and rice) a large accumulation of soil nitrate N and its subsequent disappearance upon soil saturation occurred during the transition season. This nitrate loss was associated with nitrate leaching (6.3 and 12.8 kg ha⁻¹ at the low and high altitude sites, respectively) and peaks of nitrous oxide emissions (120 and 480 mg m⁻² h⁻¹ at the low and high altitude sites, respectively). Incorporation of wheat straw at 3 Mg ha⁻¹ and/or cultivation of a nitrate catch crop during the transition season significantly reduced the build up of soil nitrate and subsequent N losses at the low altitude site. At the high altitude site, cumulative grain yields increased from 2.35 Mg ha⁻¹ with bare fallow during the transition season to 3.44 Mg ha⁻¹ when wheat straw was incorporated. At the low altitude site, the cumulative yield significantly increased from 2.85 Mg ha⁻¹ (bare fallow) to between 3.63 and 6.63 Mg ha⁻¹, depending on the transition season option applied. Irrespective of the site and the land use option applied during the transition season, systems N balances remained largely negative, ranging from −37 to −84 kg N ha⁻¹. We conclude that despite reduced N losses and increased grain yields the proposed options need to be complemented with additional N inputs to sustain long-term productivity.     

Yield formation and tillering dynamics of direct-seeded rice in flooded and nonflooded soils in the Huai River Basin of China

Water shortage in the Huai River Basin prompts farmers to adopt water-saving technologies such as direct-seeded nonflooded or aerobic rice. Different cultivation practices impact on tiller growth and development. Improved insight into tiller dynamics is needed to increase yield in these production systems. We conducted field experiments with four direct-seeded rice varieties under flooded and nonflooded conditions in Mengcheng county, Anhui province, in 2005–2006. The soil water content in the nonflooded treatment varied between saturation and field capacity. Yields in nonflooded soil ranged from 3.6 to 4.7 t ha⁻¹, and did not differ significantly from yields in flooded soil that ranged from 3.6 to 5.1 t ha⁻¹. Variety had a significant effect on biomass, yield, panicle number, spikelet number, grain weight, and grain filling percentage. Panicle number was the main factor limiting yield, resulting from a low tiller emergence frequency and a low fraction of productive tillers in both the flooded and the nonflooded soils. On average, the panicle number was 159–232 m⁻², including 34–167 productive tillers per m² for all the varieties under the two water regimes. The contribution of productive tillers to yield varied between 7% and 47%. There were two peaks of tillers that contributed to yield, one at the low (4th or 5th) and one at the high (10th or 11th) phytomer orders. Frequencies of tiller emergence at most phytomer orders were higher in the flooded soil than in the nonflooded soil. There were no significant differences in frequencies of productive tiller emergence and contributions to yield from tillers between the soil water regimes for three of the four tested varieties. To increase yield in direct-seeded nonflooded rice production systems, both the tiller emergence frequency and the fraction of productive tillers should increase through breeding, improved crop management, or a combination.     

Reciprocal influence of crops and shallow ground water in sandy landscapes of the Inland Pampas

In regions with shallow water tables, ground water may have a positive (water supply) or negative (waterlogging or salinization) impact on crops. Reciprocally, crops can influence ground water, altering water table depth and chemical composition. We quantified these reciprocal influences along natural gradients of groundwater depth in flat sedimentary landscapes of the Inland Pampas occupied by wheat, soybean, and maize during two growing seasons (2006/2007 and 2007/2008). We correlated crop yield and groundwater depth maps at the field level and made direct plant, soil and groundwater observations at the stand level across topographic gradients. Water table level largely accounted for spatial crop yield variation, explaining 20–75% of their variance. An optimum groundwater depth range, where crop yields were highest, was observed for all three crop species analyzed (1.40–2.45 m for maize, 1.20–2.20 m for soybean, and 0.70–1.65 m for wheat). The areas within these optimum bands had yields that were 3.7, 3 and 1.8 times larger than those where the water table was below 4 m for wheat, maize, and soybean, respectively. As groundwater levels become shallower than these depth bands, crop yields declined sharply (∼0.05 kg m⁻² on average for every 10 cm increase in water table level), suggesting negative effects of waterlogging, root anoxia and/or salinity. Groundwater levels below these depth bands were associated with gradually declining yields, likely driven by poorer groundwater supply.     

The implications of land preparation, crop establishment method and weed management on rice yield variation in the rice-wheat system in the Indo-Gangetic plains

The implications of adopting alternative seeding methods for rice and wheat establishment were examined at three geographically separate sites in the rice-wheat system of the Indo-Gangetic plains, across northern India. Rice yields in cultivated plots, established by either wet or dry seeding methods, were evaluated in comparison to yields from zero-tillage plots and under conventional transplanting methods. In the same trials, the effects of crop establishment methods in wheat were assessed both on wheat yields and rice yields. Rice crop establishment methods markedly influenced the emerging weed flora and attainable yields were measured in relation to intensity of weed management. Over four years, average rice grain yields in the absence of weed competition were greatest (6.56 t ha⁻¹) under wet seeding (sowing pre-germinated rice seed on puddled soil), and similar to those from transplanted rice (6.17 t ha⁻¹) into puddled soil, and dry seeded rice after dry soil tillage (6.15 t ha⁻¹). Lowest yields were observed from dry seeded rice sown without tillage (5.44 t ha⁻¹). Rice yield losses due to uncontrolled weed growth were least in transplanted rice (12%) but otherwise large (c. 85%) where rice had been sown to dry cultivated fields or to puddled soil, rising to 98% in dry seeded rice sown without soil tillage. Weed competition reduced multiple rice yield components, and weed biomass in wet seeded rice was six-fold greater that in rice transplanted into puddled soil and twice as much again in dry seeded rice sown either after dry tillage or without tillage. Wheat grain yields were significantly higher from crops sown into tilled soil (3.89 t ha⁻¹) than those sown without tillage (3.51 t ha⁻¹), and also were elevated (5% on average) where the soil had been dry cultivated in preparation for the previous rice crops rather than puddled. The method of wheat cultivation did not influence rice yield. Soil infiltration rates in the wheat season were least where the land had been puddled for rice (1.52 mm h⁻¹), and greater where the soil had been dry-tilled (2.63 mm h⁻¹) and greatest after zero-tillage (3.54 mm h⁻¹).These studies demonstrated at research managed sites across a wide geographic area, and on farmers’ fields, that yields of dry seeded rice sown after dry cultivation of soil were broadly comparable with those of transplanted rice, providing weed competition was absent. These results support the proposition that direct seeding of rice could provide an alternative to the conventional practice of transplanting, and help address rising costs and threats to sustainability in the rice-wheat rotation. Further, analysis of patterns of long-term rainfall data indicated that farmers reliant on monsoon rainfall could prepare fields for dry direct seeded rice some 30 days before they could prepare fields for either transplanting or seeding with pre-germinated seed. Dry, direct seeding of rice contributes a valuable component of an adaptive strategy to address monsoonal variability that also may advance the time of wheat establishment and yield. Whilst the results illustrate the robustness, feasibility and significant potential of direct seeded rice, they also highlight the critical nature of effective weed control in successful implementation of direct seeding systems for rice.