Shoot zone uptake of soil-applied herbicides in some legume species

Localized placement of prometryne, linuron and diuron in the soil at the first or second shoot internodes of dwarf broad bean (Vicia faba L.) equally reduced aerial plant growth, whereas simazine and atrazine had no effect. Growth reduction also occurred when the first shoot internode of scarlet runner bean (Phaseolus multiflorus L.) in the soil was treated with all five herbicides, especially with diuron. Localized placement of these herbicides at the first or second shoot internodes of vetch (Vicia sativa L.) in the soil equally reduced aerial plant growth. Foliar injury to vetch due to placement of these herbicides in the shoot zone of the soil was markedly reduced by simultaneous treatment with trifluraiin or nitralin which prevented adventitious root development on the shoot without otherwise affecting plant growth. This lack of root development on the shoots treated with trifluraiin was associated with a marked decrease in ¹⁴C-labelled atrazine uptake, which probably accounted for the reduction in atrazine phytotoxicity. A similar explanation may account for the reduced phytotoxicity of the other herbicides in the presence of trifluraiin or nitralin.     

VERTICAL DISTRIBUTION OF HERBICIDES IN SOIL AND THEIR AVAILABILITY TO PLANTS: SHOOT COMPARED WITH ROOT UPTAKE

Summary. Turnip, lettuce and ryegrass seedlings showed toxicity symptoms following shoot exposure to atrazine, linuron and aziprotryne at soil concentrations less than would be obtained from normal field applications. Responses following shoot exposure to simazine and lenacil were much less. Root exposure to all five herbicides caused seedling death at concentrations lower than those required for 'shoot-zone' toxicity. Pronamide and chlorpropham were tested against ryegrass only and at the concentrations examined were toxic only when localized in the shoot zone. Root exposure suppressed root growth, but the shoots were able to grow normally if the soil was kept sufficiently moist. Shoots contained more ¹⁴C-atrazine at emergence after shoot exposure compared with root exposure, but there was little subsequent uptake from the shoot zone. There was extensive uptake from the root zone after emergence. In the shoot-zone treatments, concentrations in the plant were high at emergence but were rapidly diluted by plant growth, whereas with root exposure, they increased throughout the experiments. The possible significance of these results to herbicide bebaviour under field conditions is discussed.
La distribution verticale des herbicides dans le sol et leur disponibilité pour les plantes: absorption comparée par la partie aèrienne et par les radnes     

VERTICAL DISTRIBUTION OF HERBICIDES IN SOIL AND THEIR AVAILABILITY TO PLANTS: TREATMENT OF DIFFERENT PROPORTIONS OF THE TOTAL ROOT SYSTEM

Summary. Uptake of atrazine and linuron by wheat seedlings was measured from different initial distributions of herbicide in the soil. Uptake of both compounds was proportional to the fraction of the total root system exposed to herbicide-treated soil. It was calculated that under the conditions of the experiments, this factor would offset the reduction in concentration of herbicide in the soil following incorporation of 1 kg/ha to 3, 6 or 9 cm, so that after the same time, the concentrations of herbicide in the plants in the different treatments would be similar.
La distribution verticale des herbicides dans le sol et leur disponibilité pour tes plantes: traitement de différentes fractions de l'ensemble du système racinaire total     

SHOOT ZONE UPTAKE OF SOIL-APPLIED HERBICIDES*

Summary. Studies were conducted to determine the effects of herbicide placement at different zones of maize (Zea mays L.) and pea (Pisum sativwm L.) shoots below the soil surface after emergence. Soil was removed from around the shoots and replaced with herbicide-treated soil. A wax barrier ensured separate exposure of the zones to treated soil. EPTC, chlorpropham, propham and sulfallate did not affect pea shoot growth, but in maize the shoot zone adjacent to the crown root node was extremely sensitive. Treatment in this area markedly reduced growth and severely inhibited the crown roots. The difference in susceptibility between these species may he due to the location of the growing point relative to the treated soil. Shoots of maize and pea were sensitive to diuron. In maize the shoot adjacent to the crown root node and the tissue of the first internode were the most susceptible. In pea the- uppermost shoot (beneath the soil surface) was the most sensitive. Trifluralin did not affect growth of maize and pea when placed in the shoot zone after emergence, although the crown roots of maize were severely inhibited. Naptalam, dalapon and 2,4-D did not affect growth of maize under similar conditions, and of these only 2,4-D reduced growth of pea. Zone d'abiorption des tiges pour les herbicides appliqués sur h sol     

EFFECT OF 2,4-D AND PICLORAM ON TRANSLOCATION OF 14C-ASSIMILATES IN VITIS VINIEERA L.

Summary. High concentrations of 2,4-D and picloram interfered with the downward movement of ¹⁴c-assimilates infield-grown vines. The interference in translocation was appreciably greater with picloram than it was with 2,4-D, Although basipetal translocation was retarded, translocation within the treated shoots continued from the vegetative part to the clusters. Translocation of 2,4-D appeared to follow the same route as ¹⁴c-assimilatcs for the most part. Formative effects were absent on untreated grape shoots although the adjacent shoots treated with 2,4-D or picloram on the same cordons were killed; however, formative effects were evident on some of the stump sprouts which developed after the vines were harvested. The malformed leaves on the stump sprouts were twelve or more nodes from the base of the shoots, while ¹⁴c was in the more basal leaves.
Thompson Seedless (Sultanina) rootings treated with 20 000 ppm 2,4-D or picloram transported less ¹⁴C to the roots than did the controls. Treatment with either herbicide resulted in a marked increase in the labelling of the stems.
Effet du 2,4-D et du pichlorame sur la migration de métabolites marqués au ¹⁴C dans Vitis vinifera L.     

Uptake and translocation of imazethapyr in peas as affected by parasitism of Orobanche crenata and herbicide application methods

[¹⁴C]-Imazethapyr was applied as a seed treatment and at plant pre-emergence and post-emergence to peas ( Pisum sativum L.) parasitized by Orobanche crenata Forsk. Herbicide uptake increased with time regardless of the application method. Uptake reached about 98%, 89%, 81% and 94% of the total herbicide applied for the seed coating, seed soaking, pre-emergence and post-emergence treatments respectively. Herbicide translocation within the host plants consistently differed between O. crenata -infected and non-infected plants. High levels of ¹⁴C activity were accumulated by parasitic plants from the host. In non-infected pea plants, pods were stronger sinks for imazethapyr than the other parts of the plant, regardless of the application method. The herbicide distribution in the pea plant: O. crenata complex showed the same pattern regardless of the application methods. However, accumulation of radioactivity in the parasite was lower with pre-emergence and post-emergence application than with the seed treatments. In addition, radioactivity concentration in O. crenata plants was slightly higher when [¹⁴C]-imazethapyr was applied to pea seeds by coating than by soaking.     

Distribution of 14C-glyphosate in legumes parasitised by Orobanche crenata

Absorption and translocation of ¹⁴C-glyphosate was evaluated under controlled conditions in peas ( Pisum sativum L.) and broad beans ( Vicia faba L.) parasitised or not by crenate broomrape Orobanche crenata Forsk.). Absorption increased with time up to 12 days after treatment, and reached about 50% of the ¹⁴C-glyphosate applied. Three days after treatment 70–85% of the total herbicide absorbed had been translocated out of the treated leaflet. There was no consistent differ ence in absorption and translocation between infected and non-infected plants 12 days after treatment. The ¹⁴C-glyphosate concentration in the root system increased with time in broad beans. In peas it remained more or less constant from 3 days after treatment. Generally, pods were stronger sinks for glyphosate than other parts of the plant. Herbicide accumulation in broomrape increased with its growth stage, and the parasite was a much stronger sink for glyphosate than the legume root system.     

Root exudation of diclofop-methyl and triasulfuron from foliar-treated durum wheat and ryegrass

In this study, we evaluated the release of diclofop-methyl and triasulfuron from the roots of foliar-treated ryegrass and wheat. The study with ¹⁴C-diclofop-methyl indicated a basipetal translocation of foliar-applied herbicide in wheat and ryegrass. No root exudation from ¹⁴C-diclofop-methyl-treated wheat plants was observed, while 20 days after treatment (DAT) 0.2–0.9% of radioactivity absorbed by ryegrass was found exuded in the growing medium. Root exudation was stimulated three to six times by the presence of untreated wheat or ryegrass sharing the growing medium with diclofop-methyl-treated ryegrass. No subsequent uptake of exuded radiolabel by untreated plants (ryegrass or wheat) in the same pot with ¹⁴C-diclofop-methyl-treated ryegrass was observed. The study with ¹⁴C-triasulfuron indicated a basipetal translocation of foliar-applied herbicide in wheat and ryegrass and also into the growing medium. By 20 DAT, 0.5–4.2% of radioactivity absorbed by wheat or ryegrass was found exuded in the growing medium. The presence of untreated plants (wheat or ryegrass) in the same pot as triasulfuron-treated ryegrass or wheat induced exudation seven to 32 times more. The study also revealed a subsequent uptake of exuded compounds by untreated wheat or ryegrass sharing the medium of ¹⁴C-triasulfuron-treated plants. This study has demonstrated for the first time that the root exudation of exogenous compounds can be related to plant arrangement in pots. The implication is that herbicide root exudation and transfer, a form of allelopathy, could be significant in the field. A precise estimation of environmental fate, unexpected ecological side effects and residual activity of herbicides may require quantification of such exudation.     

莠去津土壤残留生物修复菌肥作用原理与田间应用

莠去津是玉米田应用的优秀除草剂品种,然而由于其在土壤中残留时间长,常对轮作后茬敏感作物造成严重毒害。采用生物修复菌肥做种肥、结合菌肥拌种和叶面喷施方法,研究对玉米后茬旱直播水稻生长发育及药害修复机理,对土壤中莠去津残留量、水稻生长和生理指标、土壤酶活性进行测定。结果表明:颗粒菌肥做种肥+粉剂菌肥拌种+水剂菌肥叶面喷施是莠去津土壤残留毒害修复的最佳方法,土壤中莠去津含量在喷施菌肥后7 d从施用菌肥前的0.9 mg/kg下降到0.1 mg/kg,水稻叶片叶绿素含量显著增加33.74%,超氧化物歧化酶和过氧化物酶酶活性分别显著提高23.39%和92.57%,丙二醛含量则显著降低48.01%;水稻株高、地上部鲜重、干重分别比对照显著增加22.33%、67.51%和74.80%,根系鲜重和干重分别比对照显著增加33.98%和55.43%;土壤磷酸酶、脲酶及纤维素酶含量分别显著增加49.17%、528.65%和35.21%。     

SITE OF UPTAKE OF SOIL-APPLIED HERBICIDES*

Summary. We conducted studies to determine the effects on corn (Zea mays L, var. Indiana 654) and pea (Pisum sativum L. var. Alaska) of localizing various herbicides in the soil, using a double plastic pot technique which ensured separate exposure of the root and shoot zones of the plants to treated soil. Effects on corn and pea were similar in relation to site of uptake. 2,4-D-amine, naptalam, simazine, diuron and dalapon-sodium entered primarily through the roots. Some shoot entry and also severe inhibition of roots occurred in soil treated with 2,4-D and naptalam; these were noticed only to a slight extent with the other three herbicides. EPTC, chlorpropham and trifluraiin were most effective when applied to the shoot zone. Little effect on foliage growth was evident when the root zone alone was treated. However, roots in treated soil were severely inhibited by these three herbicides. Dinoseb displayed a contact type of action, injuring both shoots and roots. Treatment of both zones had an additive effect. Entry of chlorthal-methyl which was tested on a susceptible species, sorghum (Sorghum vulgare Pers.) was mainly through the shoot, with only a slight effect on top growth when roots alone were treated. Roots in treated soil were slightly inhibited. Localisation de l'absorption des herbicides appliqués sur le sol     

Increased herbicidal activity of triazine herbicides by piperonyl butoxide

Piperonyl butoxide (PB) is a known Synergist which enhances the activity of insecticides by inhibiting their biotransformation to less active products. We have evaluated the possible use of PB as a herbicide synergist using triazine herbicides in sensitive, tolerant, and resistant plants. The effects of PB, triazine herbicides, and their combinations were examined in whole plants as well as in chloroplasts isolated from triazine-sensitive (S) and -resistant (R) weed biotypes. PB itself, applied postemergence (0.1–0.5%, v/v), was slightly toxic to the plants tested. However, foliar application of PB combined with atrazine, terbutryn or prometryn to maize seedlings significantly increased the phytotoxicity of the herbicides. Low rates of atrazine, prometryn, and terbutryn in a tank-mixture with PB, effectively controlled Solatium nigrum L. and Abutilon theophrasli Medik. PB enhanced atrazine efficacy in both S and R biotypes of Lolium rigidum Gaud. The synergistic effect of PB was evident also in vitro when atrazine and methabenzthiazuron were used to inhibit photosystem II electron transport in chloroplasts isolated from resistant weeds. These data demonstrate the potential of PB as a herbicide synergist and its possible utilization as an aid for improving the activity of triazine herbicides in sensitive, tolerant and resistant plants.     

Histological studies on the effects of benzoylprop ethyl and flamprop methyl on growth and development of wild oats*

Wild oats were trealed with benzoylprop eihyl and flamprop methyl alone or in combination with other herbicides and the effects on plant growth studied using histological techniques, Benzoylprop ethyl caused severe reductions in the growth of both the leaves and internodes of treated plants, Evidence was obtained which indieaies that cell division as well as cell elongalion was inhibited. The effects of flamprop methyl on plant growth were similar lo those observed for benzoylprop ethyl. Both compounds interfered with normal differentiation of vascular tissues which could account for retarded development of the higher internodes and shoot apex of treated plants. Of the various herbicides applied in combination with the wild oat herbicides, 2,4—D was more antagonistic than MCPA. while bromoxynil was the least antagonistic. Plants treated with benzoylprop ethyl plus 2.4—D had increased cell, leaf and internode lengths compared to plants treated with benzoylprop ethyl alone. Reductions in cell and internode lengths of plants treated with the herbicide combinations were not always proportional lo dry weight reductions.     

Measurement and simulation of the movement and degradation of atrazine and metribuzin in a fallow soil

The movement and persistence of atrazine and metribuzin, in a sandy loam soil following application in spring, was simulated using two models. The first model, based on the physical laws describing water and solute movement and using measured values of soil hydraulic properties, underestimated herbicide mobility in the soil and predicted too rapid drying of the deeper soil layers. The accuracy of the simulations was improved by empirically reducing the measured hydraulic conductivities by a factor of 4. This probably reflects the difficulties of obtaining reliable measurements of soil hydraulic properties. A second and simpler model, which simulated water and herbicide movement using mobile and immobile water categories, accurately predicted soil water contents. It tended to underestimate herbicide movement at short times after application, and to overestimate movement later in the experiments. A comparison of different methods of simulating herbicide degradation showed that prediction of degradation rates in the field from laboratory data can be unsatisfactory with some compounds.     

Influence of Compost Addition to Soil on the Behaviour of Herbicides

The transformations of eight herbicides (atrazine, simazine, terbutryn, pendimethalin, carbetamide, 2,4-D, metsulfuron-methyl and dimefuron) in soil after compost addition were monitored during long-term laboratory incubations. The herbicides were applied to soil, compost and soil-compost mixtures. Herbicide sorption, their kinetics of mineralisation and the extractability of residues were compared in the different treatments. Compost addition to soil generally decreased herbicide mineralisation and favoured the stabilisation of herbicide residues. A fraction of the stabilised residues remained extractable and potentially available. However, most of them were unextractable and formed bound residues. Sorption could be at the origin of a kinetically limited biodegradation, mainly for the most highly-sorbed herbicides (atrazine, simazine, terbutryn, pendimethalin and dimefuron). Compost addition had little effects on the less sorbed herbicides (carbetamide, 2,4-D and metsulfuron- methyl). © 1997 SCI.     

除草剂莠去津对环境的污染及治理

本文综述了除草剂莠去津的环境污染情况及其对水生生物、陆生动物和植物的影响,并提出了制定相关标准,合理施药,使用替代药剂,提高农民的施药技术水平和生物修复的治理措施,更好地发挥其在农业生产上的积极作用。     

Réduction de ľutilisation des herbicides dans le maïs-grain par une application ďherbicides en bandes combinée à des sarclages mécaniques

Ce projet a permis ?évaluer des programmes de désherbage combinant ?application ?herbicides en bandes et les sarclages mécaniques comme méthode alternative à?utilisation intensive ?herbicides dans le maïs-grain (Zea mays L.). Les résultats des travaux menés au Québec en 1991 et 1992 indiquent que ?application ?herbicides en bandes (atrazine à 1,0 kg m.a. ha^?1+métolachlore à 1,9 kg m.a. ha ^?1 en post-levée précoce) sur le rang (40% de la surface totale cultivée) suivie par des sarclages mécaniques sélectifs sur toute la surface procurent des rendements de maïs-grain équivalents a ceux obtenus là où les herbicides ont été appliqués sur toute la surface cultivée. Il est donc possible de réduire la quantité?herbicides utilisée dans le maïs-grain sans diminuer son rendement. La répression mécanique des mauvaises herbes a été plus efficace en 1991, qui a été une année sèche comparée à 1992 qui a été froide et pluvieuse. Il ressort de cette étude qu'un traitement combinant une application ?herbicides en bandes et deux sarclages sélectifs sur toute la surface à 2 et 4 semaines après ?émergence du maïs-grain procure à la fois un désherbage adéquat des adventices et un rendement optimum du maïe. Reduced use of herbicides in corn through herbicide-banding combined with cultivations The efficacy of various weed management systems utilizing banded applications of herbicides and mechanical cultivations were investigated in order to find alternatives to intensive herbicide use in grain maize (Zea mays L.) production. In experiments conducted in Quebec in 1991 and in 1992, banded applications of atrazine (1.0 kg a.i. ha ^?1) and metolachlor (1.9 kg a.i. ha^?1) on maize rows (40% of field area) combined with post-emergence cultivations, achieved a similar grain yield to that obtained when herbicides were broadcast over the whole field. In this project, decreasing herbicide was not accompanied with decreasing maize yield. Mechanical weed control was more effective in 1991, a drier year than 1992 which was cool and rainy. A banded application of herbicides followed by cultivations at 2 and 4 weeks after maize emergence provided commercially acceptable weed control and crop yield.     

ABSORPTION AND TRANSLOCATION OF HERBICIDES BY THOMPSON SEEDLESS (SULTANINA) CRAPE, VITIS VINIFERA L.

Summary. Absorption and translocation of ¹⁴C-labelled herbicides applied in culture solutions lo the roots and in concentrated drops to mature leaves of 'Thompson Seedless' (Sultanina) cuttings were studied. Neither 2,4-D nor paraquat, and only traces of 2,4,5-T translocated from the roots to the shoot. Dicamba, amitrole, diuron, monuron, simazine and atrazine readily moved from the roots to the shoots. The substituted ureas and s -triazines were mobile only in the xylem or cell walls (apoplast), while dicamba and amitrole were mobile in the apoplast and symplast (living protoplasm). Both 2,4-D and 2,4,5-T moved almost entirely in the symplast. Paraquat moved only slightly in either apoplast or symplast. Stability of the herbicides in the plants varied, as judged by the detection of label in ethanol-insoluble products. Dicamba was considerably more stable than 2,4,5-T which, in turn, was more stable than 2,4-D. Diuron was more stable than either atrazine or simazine. Amitrole largely was converted into ethanol-insoluble products in 30 days. Paraquat was not extractable with ethanol.
Absorption et migration d'herbicides chez une vigne: Thompson Seedless (Sultanina), Vitis vinifera L.     

Effects from simulated rainfall on shoot-zone uptake of herbicides

In glasshouse experiments, atrazine, simazine, lenacil and linuron applied to the soil surface were phytotoxic to turnip seedlings which had emerged from a depth of 1–5 cm when 3 mm artificial rainfall was applied at the time of seedling emergence or shortly afterwards. When rainfall was applied on 2 or 3 consecutive days, the herbicides were in general more phytotoxic. As the delay between emergence and commencement of surface watering increased, however, the response of the seedlings decreased. Studies with [¹⁴C]-atrazine showed that at emergence the seedlings contained a small amount of herbicide which increased considerably with surface watering. Relatively high concentrations were attained when rainfall was applied while the seedlings were small, but as the interval between seedling emergence and rainfall increased, the same uptake resulted in lower shoot concentrations, Atrazine extraction from the soil showed little movement from the surface 1.0 cm, suggesting uptake via the stem. The observed responses of the other three herbicides are explained by assuming the same pattern of uptake as that recorded for atrazine.     

土壤湿度对三氮苯类除草剂药效的影响及优化方案

以稗草为生物测定材料,运用二次正交旋转组合设计,以土壤湿度和除草剂用量二因子为决策变量,对稗草的抑制率为目标函数,研究土壤湿度对三氮苯类除草剂药效的影响。结果表明,适当的土壤水分是三氮苯类除草剂发挥药效的重要因素,药效随土壤湿度的提高而提高。不同的土壤湿度对不同除草剂药效影响各异,高湿条件下,湿度差异对药效影响大小依次为嗪草酮、西草净、扑草净、莠去津,低湿条件下则相反。除草剂用量与土壤湿度存在最佳发挥药效的组合。     

Effects of the safeners CGA 154281, oxabetrinil and fenclorim on uptake and degradation of metolachlor in corn (Zea mays L.) seedlings

Summary. The influence of three crop safeners on the uptake and degradation of ¹⁴C-metolachlor was investigated in two corn varieties. Following application of herbicide and safener together to seedling shoots the concentrations of non-metabolized ¹⁴C-metolachlor in the tissues was found to be lower in the tolerant variety LG 9 than in the susceptible variety 211A. The difference between varieties was due to differences in both uptake and degradation of ¹⁴C-metolachlor.
Following shoot application most of the radioactivity was retained in the coleoptile and the mesocotyl. Two hours after application 95% of the herbicide had been degraded in coleoptiles and mesocotyls, whereas approximately 20% of non-metabolized ¹⁴C-metolachlor was present in the enclosed developing shoot leaves. In both corn varieties the safener CGA 154281 caused a substantial lowering of tissue levels of parent ¹⁴C-metolachlor. This was primarily due to an enhanced degradation. Glutalhione- S -transfer-ase (GST) enzyme activity in shoot tissues was found to be enhanced in both varieties by CGA 154281. Oxabetrinil and fenclorim were less effective than CGA 154281 both in reducing tissue levels of non-metabolized ¹⁴C-metolachlor and in enhancing GST activity in either variety.