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Materials and methods: The number of snails on citrus trees was monitored and counted 10 days after the application of the treatments, and at an interval of 6–8 days up to harvest time.
Results: In the first study, the mineral oil and repellent paint treatments reduced a number of snails best. In the second study, using metaldehyde and mineral oil barrier, again the mineral oil barrier reduced snails best. The cost of each treatment during one season per hectare was calculated at 55, 153, 124 and 120?$/ha for mineral oil, iron phosphate, snail-repellent paint and metaldehyde, respectively.
Conclusions: Mineral oil is an effective alternative for chemical compounds for reducing access by H. candeharica to citrus trees. 相似文献
AMF colonization was evaluated by visual observation of AMF in fine roots of eight herbaceous plants. The level of mycorrhizal colonization varied between plants. Astragalus corrugatus and Hippocrepis areolata showed the highest mycorrhizal performance. The relative spore number was significantly different across rhizosphere soils. Statistical analysis showed a clearly positive correlation between the number of spores and plant-mycorrhizal intensity.
For microbiological parameters, our results showed that mycorrhizal plants improved significantly the various microbiological parameters. Rhizosphere soils of Astragalus corrugatus and Hippocrepis areolata presented the necessary microbial densities and microorganisms more stable compared to unplanted soil. This study allowed obtaining a new result that challenges us about the need for efficient management of natural resources in the objective of nature conservation. 相似文献
Materials and methods: Four treatments (root drench, root damaging and drench, root drench with matrine, and flower spray) were applied to inoculate alfalfa with two fluorescent-tagged rhizobia, Ensifer meliloti LZgn5f (gn5f) and Ensifer meliloti 12531f (12531f), at three different growth stages; bud, flower and pod stages. The migration and colonisation dynamics of the two fluorescent tagged rhizobia strains were monitored using UV lamp detection and a stereo fluorescence microscopy.
Results: The results showed that both rhizobia strains mainly colonised the roots and could migrate to aerial tissues. In aerial tissues, when alfalfa plants were inoculated during the bud stage, both rhizobia strains mainly colonised the leaves and stems; during the flower stage, a spray inoculation treatment resulted in more 12531f colonising reproductive tissues, while during the pod stage, more rhizobial strains gn5f colonised seeds using the root drench with matrine treatment.
Conclusions: These results indicate that endophytic rhizobia are natural inhabitants of internal regions of roots, stems, leaves and that the endophytes may arise from reproductive tissues, such as seeds. Understanding the population dynamics of endophytic rhizobia in alfalfa would considerably improve the survival of target rhizobia during seed transfer. Combining target endogenous rhizobial species with good alfalfa seed varieties may lead to the development of a novel breeding method. 相似文献
Materials and methods: Tomato plants were subjected to three organic nutrient solutions, which consisted of different mixtures of several OMRI (Organic Materials Review Institute) certified nitrogen fertilizers of industrially processed residues: Treatment I: solid and soluble liquid fertilizers of animal raw materials, natural potassium sulphate-non-synthetic, and calcium chloride; Treatment II: solid and soluble liquid fertilizers of animal raw materials, by-product of marine raw material (soluble liquid), natural potassium sulphate-non-synthetic, and calcium chloride; and Treatment III: solid fertilizers of animal raw materials, natural potassium sulphate-non-synthetic, and calcium chloride to 100% [0-30 days after transplanting (DAT)], 125% [31-80 DAT], and 150% [>81 DAT]. The Steiner solution (SS) was used as a control (Treatment IV).
Results: Yield did not differ between organic and conventional treatments, ranging from 3.04 to 3.35 kg m-2 while fresh weight in organic treatments was 3.14 compared to 3.2 kg m-2 in plants fed with the SS. No significant differences in plant height or fruit quality were found. The application of organic fertilizers positively affected the total hydrolysable and condensed polyphenols of tomato fruits compared to the control. Twelve phenolic compounds were identified, highlighting 3-Caffeoylquinic acid, salvianolic acid and 5,6-Dihydroxy-7,8,3’,4’-tetramethoxyflavone (Treatment I) and Medioresinol (Lignan) (Treatment II).
Conclusions: The results indicated that organic fertilization through animal-based fertilizer application is a feasible alternative for grape tomato production under greenhouse conditions. 相似文献
Materials and methods: In this study, 52 strains of Pseudomonas syringae pv. actinidiae were isolated from 68 stem samples of kiwi plant (cv. Hongyang & Jinkui). Following polymerase chain reaction (PCR) analysis, 15 isolates belonging to biovar 3 were identified, one of which was named XWY0007 and used as the target strain to isolate the phages. Thirty-six phages were isolated and purified from a total of 51 surface water samples collected in Shanghai. All phages were identified by transmission electron microscopy (TEM) and their host ranges were evaluated. Three phages, designated φXWY0013, φXWY0014 and φXWY0026 were selected and further characterised using one-step growth curve and stability at different temperatures and pH.
Results and conclusions: The isolated phages are promising for use as antimicrobials against bacterial canker in kiwi. This report is regarding Pseudomonas syringae pv. actinidiae and its phages from major areas of kiwifruit cultivation. 相似文献
Materials and methods: The growth of roots and root hairs as well as uptake of macro- and micronutrients of six spring wheat varieties was compared in solution culture under P stress and P abundance and in a low fertility soil.
Results and conclusions: Root length and surface area under P stress were significantly positively correlated with that in the low fertility soil, while no such correlation was apparent for root hair length and density. In absolute terms, the root length, surface area, root hair length and density of spring wheat varieties were substantially higher in soil than in solution culture, while the concentration and uptake of macro- and micronutrients in soil differed from solution culture in a complex way. The early uptake of macro- and micronutrients was intimately associated with root length and surface area as well as root hair length and density in soil but not in solution culture. Therefore, root length rather than root hair traits in low-P solution may be used to screen early root growth vigor in soil and thereby high nutrient uptake of wheat in low fertility soil. 相似文献
Materials and methods: Endophytic bacteria were isolated from root, stem, and leaf of ginseng from different sites and genotype in China and Korea, screened based on their beneficial properties as PGPB. Nine bacterial isolates were selected according to their plant growth properties including soluble phosphate and potassium, ammonia, auxin and siderophore producing, ACC deaminase, and antagonistic pathogen as well. Changes in ginseng after PGPB inoculation were evaluated with respect to the non-inoculated control.
Results and Conclusions: The PGPB isolates were identified as genera Bacillus, Lysinibacillus, Rhizobium, Stenotrophomonas, Erwinia, Ochrobactrum, Enterobacter and Pantoea based on 16S rRNA sequences. Inoculation of G209 and G119 increased not only plant height, root length, fresh weight, and dry weight, but also root activity and the amount of ginsenosides significantly. In particular, using the Illumina Miseq platform, the native bacterial community of rhizospheric soil maintained high community diversity and increased abundance of specific bacteria. Therefore, they may be play a crucial role in sustainable ginseng cultivating in farmland. 相似文献
Materials and methods: Rootstocks B9 and M9 with a size suitable for grafting (6-10 mm stem diameter, termed rootstocks), and smaller sized rootstocks (<5 mm stem diameter, termed transplants) of B9, M9, M26, MM106 and Antonovka were inoculated with N. ditissima at different times, either with contaminated map pins or with spore suspensions. In addition, the rootstocks were either defeathered (side shoots removed), topped (top shoot headed) or both, to create wounds that would normally occur during propagation, while wounds on transplants were made by removing leaves.
Results and discussion: One month after inoculation, slightly sunken canker lesions had developed around the inoculation points of the map pins or wounds. No lesions developed on the non-inoculated controls. Map pin inoculation resulted in 30% to 89% infection and spore suspension sprayed on wounds from 5% to 45% infection. When the cankered areas were split open, brown lesions with necrotic tissue due to infection by N. ditissima appeared. The transplants of M9, M26 and MM106 inoculated with contaminated map pins in 2014 developed necrosis on 40% to 67% of the plants, but there were no differences in the incidence or severity among the different types. On the transplants of B9, Antonovka and M9 inoculated in 2015, there was more necrosis on B9 (42%) than on Antonovka (11%) and more sporulating lesions on B9 (29%) than on M9 (9%) or on Antonovka (4%).
Conclusion: It can be concluded that rootstocks used for apple trees may become infected by N. ditissima, and wounds should thus be protected during propagation. 相似文献
The review also summarises complementary genomic tools useful in RYMV disease resistance breeding.
Successful breeding of rice for RYMV resistance depends on the availability of genes for stable resistance, knowledge of the genetics of the host and, the availability of efficient phenotyping and pathotyping methods, and understanding of the genes involved and their pattern of inheritance. Information presented in the review can serve as a reference guide for rice breeding emphasising RYMV resistance, high yields and farmers-preferred traits. 相似文献
While E. fetida showed no avoidance towards the digestate products (negative avoidance, meaning that the tested products were preferred, of 80 to 100 %), A. caliginosa rejected the crude biowaste digestate compost in higher concentrations (avoidance of 45 %), but not the agglomerated or pelletized variants. A clear weight gain of up to 25 % was observed only for individuals of E. fetida. The developmental stages of the worms were not crucial for the outcome of the avoidance test.
Based on the results of this study the application of biowaste products on arable land cannot be recommended without constraints. 相似文献
Applying the N fertilizer after high rainfall and transient waterlogging (tactical N application) increased GY and protein percentage of grain compared to applying all of the N fertilizer at sowing. Where transient waterlogging was not frequent, applying the N after waterlogging was not always better than applying part of the N according to growth stage of the crop or according to fixed times after sowing. When the crop was water-logged three or more times, N uptake by the crop at anthesis and apparent fertilizer N recovery in the crop was substantially increased by applying the N after waterlogging compared to applying the entire N at sowing. This study found that a tactical N management strategy for the HRZ of SWA is to apply some N at sowing with subsequent applications made after heavy rainfall that leads to transient waterlogging. Split N fertilizer applied either according to time after sowing or to growth stage of the crop was equally effective for increasing GY in situations where waterlogging was less frequent.
The observation from these experiments, that grain yield increases due to splitting the N dose were associated with increases in ear numbers, lead to a further set of experiments where plant population was increased in conjunction with N applied after waterlogging events. The combined strategy of increased plant population with strategic N application decreased the amount of N required for maximum GY where more than 3 heavy rainfall events occurred in a growing season.
One practical outcome of this research is to indicate that farmers can withhold applications of N fertilizer after sowing in seasons when transient waterlogging does not occur. 相似文献
Small but significant losses of sulphur occurred during oven drying. Losses from shoots ranged from 4.2 to 13.9 μgS/g dry weight, which represented from 0.38 to 0.66% of the total sulphur content of the shoot. Losses from roots ranged from 12.0 to 47.8 μgS/g dry weight, representing 0.82 to 1.77% of the total sulphur content of the root. Decreases in supply of nitrogen, phosphorus, potassium or sulphur generally decreased the amount of volatile sulphur lost by oven drying shoots. Losses from roots generally decreased when supply of sulphur decreased, but increased when nitrogen and potassium supply decreased, and were generally unaffected by phosphorus supply.
The organic sulphur concentration in the tissue was linearly correlated with absolute losses of sulphur (r = 0.799** for shoots; r = 0.822** for roots), the amount of sulphur lost per unit dry weight (r = 0.469* for shoots; r = 0.381* for roots) and the percentage of the total sulphur released as volatile sulphur (r = 0.937** for shoots; r = 0.970** for roots). By contrast, the total sulphur concentration in the tissue was linearly correlated only with the amount of sulphur lost per unit dry weight ( r = 0.704** for shoots; r = 0.723** for roots). 相似文献
One decade after mechanical crushing of shrubs for grassland restoration, soil amino sugar nitrogen (N) values and patterns for resprouted honey mesquite and undisturbed shrubs did not differ from one another according to a repeated measures analysis of variance. However, the concentrations in samples of the surficial 30-cm of soil around undisturbed and resprouted shrubs combined differed statistically at 161 parts per million (ppm) at trunk, 100 ppm midway between trunk and dripline, 78 ppm at the dripline, and 46 ppm beyond the dripline.
The results indicate that soil N fertility remained stable, but with decreasing levels at regular, radial sample points extending from the trunk to beyond the dripline, around mesquite shrubs that resprouted 10 years after mechanical crushing of tops for grassland restoration. 相似文献
The synergistic effect of bacteria and humic fertilizer was evidenced by increase in N and chlorophyll contents and in decreased nitrates content in lettuce leaves. Humic fertilizer resulted in decreased nitrates concentration in plants, whereas bacillus (B). subtilis No. 2 increased total N and chlorophyll contents. Results indicated that the application rate of humic fertilizer may be reduced when B. subtilis No. 2 is also applied. 相似文献
Abbreviations: PUE: phosphate use efficiency; CAT: catalase; POD: peroxidase; SOD: superoxide dismutase; DMSO: dimethyl sulphoxide; MDA: malondialdehyde; TOPSIS: technique for order preference by similarity to an ideal solution; MCDM/MADM: multi-criteria (or attribute) decision making 相似文献
Abbreviations: CAT: catalase; EL: electrolyte leakage; IPU: isoproturon; LP: lipid peroxidation; MDA: malondialdehyde; NR: nitrate reductase; POD: guaiacol peroxidase; SOD: superoxide dismutase; TCA: trichloroacetic acid 相似文献
Abbreviations: ATS: ammonium thiosulfate; DCD: dicyandiamide; ENP: encapsulated nitrapyrin; MIP: maleic-itaconic polymer; NP: nitrapyrin; UAN: urea-ammonium nitrate liquid fertilizer 相似文献
Abbreviations: Ion exchange resin membrane (RM); nitrogen (N); phosphorus (P); potassium (K), sulfur (S), field capacity (FC) 相似文献
In pea, NRA was maximum at pod maturity stage and minimum at flowering stage. In sorghum plant there was gradual increase in NRA upto grain formation followed by a fall in activity at maturity.
Nitrogen treatment as nitrate and ammonia significantly increased nitrate reductase activity over control in both pea and sorghum. Treatment with potassium nitrate was found to stimulate more NRA in pea than with ammonium sulphate. In sorghum, both forms of nitrogen did not differ much in their influence on NRA.
Influence of moisture stress in reducing NRA was more clear in sorghum, a C4 plant than in pea, a C3 plant. In general, control plants recorded low NRA in both the crops when compared to nitrogen treated plants except at pod formation stage in pea. 相似文献