Broadleaved weed control in wide-row soybean (Glycine max) using conventional and glyphosate herbicide programmes

A field study was conducted in 2001 and 2002 at Blackville, SC, to evaluate broadleaved weed control and economics of conventional and glyphosate-containing herbicide programmes in glyphosate-resistant soybean planted in 97-cm-wide rows. Treatments included chlorimuron plus sulfentrazone or chlorimuron plus metribuzin applied pre-emergence followed by post-emergence applications of lactofen or glyphosate 4 wk after soybean emergence (WAE). Other treatments were glyphosate applied 4 WAE, sequential applications of low dose glyphosate applied 2 and 4 WAE, and a non-treated control. Pre-emergence herbicides followed by glyphosate, controlled Ipomoea lacunosa L. 8 WAE. I. hederacea var. integriuscula Gray control with pre-emergence herbicides followed by glyphosate was 100% with similar control from chlorimuron plus sulfentrazone followed by lactofen, whereas control following the single glyphosate application was 84%. Amaranthus palmeri S. Wats. control 9 WAE was 100% following single or sequential glyphosate applications, while control ranged from 76% to 96% with pre-emergence herbicides followed by lactofen. However, early season weed interference when a single application of glyphosate was delayed until 4 WAE reduced soybean yields an average of 389 kg/ha compared to pre-emergence herbicides followed by glyphosate. Based on mean contrasts, pre-emergence herbicides followed by glyphosate had greater gross profit margins than a single glyphosate application alone, but were similar to sequential glyphosate applications. Gross profit margins from pre-emergence herbicides followed by lactofen were not different from glyphosate alone.     

Soil-applied herbicide use in wide- and narrow-row glyphosate-resistant soybean (Glycine max)

A field study was conducted in 2001 and 2002 at Blackville, SC, to compare the effectiveness of soil-applied herbicide programmes followed by glyphosate with sequential glyphosate applications in 19- and 97-cm row soybean. Treatments included pre-emergence applications of flumetsulam, chlorimuron plus sulfentrazone, metribuzin, or flumioxazin all applied with S-metolachlor with a subsequent application of glyphosate applied 5 wk after soybean planting (WAP). Additional treatments included sequential applications of glyphosate at 3 and 5 WAP and a non-treated control. Weed control was comparable in wide and narrow rows, and soil-applied herbicides were similar to sequential glyphosate applications in either row width. Amaranthus palmeri S. Wats., Digitaria sanguinalis (L.) Scop., and Cyperus rotundus L. control with all herbicides was 98–100%. All herbicide programmes provided at least 92% Ipomoea hederacea var. integriuscula Gray and I. lacunosa L. control. S-metolachlor plus flumetsulam followed by glyphosate provided 99% Senna obtusifolia (L.) Irwin and Barneby control, which was superior to the 88% and 90% obtained with chlorimuron plus sulfentrazone and metribuzin programmes, respectively. Flumioxazin and sulfentrazone caused up to 12% soybean injury. In 2001, soybean yields and gross profit margins were similar for soil-applied programmes followed by glyphosate and sequential glyphosate applications in wide and narrow rows. Conversely, soybean yields and profit margins in 2002 averaged across soil-applied programs were 380 kg/ha and $50/ha superior to sequential glyphosate applications in wide rows, but were similar in narrow rows. This research indicates a highly efficacious soil-applied herbicide program followed by glyphosate provides similar weed control to sequential glyphosate applications in wide- and narrow-row soybean; however, a soil-applied herbicide programme followed by glyphosate may improve yields and returns of wide-row soybean over sequential glyphosate applications alone.     

Broadening weed control with imazamethabenz combinations

Broad-spectrum weed control by use of imazamethabenz (AC 222 293) in herbicide combinations was investigated in the greenhouse. Imazamethabenz at rates ranging from 0·1 to 0·4 kg a.i./ha effectively controlled wild oats (Avena fatua L.). Additional control of redroot pigweed (Amaranthus retroflexus L.) was achieved when imazamethabenz, plus octoxynol surfactant, was applied in combination with MCPA (amine or ester) or commercial MCPA mixtures (bromoxynil/MCPA and cyanazine/MCPA). Only inclusion of propanil/MCPA in the combination reduced wild-oat control by imazamethabenz at the lower rate (0·1 kg/ha). Control of green foxtail (Setaria viridis (L.) Beauv.) was also obtained by use of fenoxaprop-ethyl in combination with imazamethabenz (plus octoxynol). Three-way mixtures of imazamethabenz (or plus octoxynol), MCPA alone or mixture, and fenoxaprop-ethyl may provide practical control of grass and broad-leaved weeds in the field.     

Cynodon dactylon control with tillage and glyphosate

Field experiments were conducted during two cropping seasons at two sites in Botswana to determine the efficacy of combining glyphosate and tillage in controlling C. dactylon. Treatments consisted of no-tillage, single and double ploughing during winter and spring plus the application of glyphosate at 0, 1.08, and 2.16 kg ha⁻¹ 3–4 weeks after the last ploughing. Visual assessment indicated higher levels of grass control from combining glyphosate and tillage than when these elements were applied separately. However, biomass data indicated that either double ploughing or glyphosate application controlled C. dactylon. A single ploughing followed by glyphosate at 2.16 kg ha⁻¹ after a regrowth of C. dactylon may provide an effective and affordable control method to small-scale farmers.     

几种长残留除草剂对后茬作物甜菜的影响

1992-2003年进行了模拟普施特和豆磺隆土壤残留对后莅作物甜菜的安全性试验。研究结果表明，普施特低剂量62．5mL／hm^2。即对甜菜有严重药害，甜菜苗鲜重降低90．9％；普施特125mL／hm^2以上各处理，甜菜苗全部死亡。豆磺隆低剂量0．25g／hm^2对甜菜的保苗株数、苗高、苗鲜重影响较小，但产量降低17．9％；高剂量2、4g／hm^5处理药害严重，甜菜死苗率90％以上，产量降低92％以上。广灭灵975-3000mL／hm^2各剂量处理施药后12个月，对甜菜均无明显药害。株鲜重和产量与不施药对照相近。金豆1000、1500mL／hm^2低剂量处理，施药后12个月对甜菜药害轻微，可以种植，24个月后可以安全种植甜菜；金豆2000、3000mL／hm^2高剂量，施药后12个月对甜菜有明显药害，不可以种植；24个月后药害轻微，可以种植甜菜。金普施特低剂量1250mL／hm^2和倍量2500mL／hm^2处理对甜菜均有药害。施药后12个月药害重于24个月和36个月。但36个月后高、低剂量下仍有药害，仍不能安全种植甜菜。阔草清60、120、180g／hm^2各处理，施药后12个月均有较重药害．不能种植；施药后24个月时，60g／hm^2低剂量处理药害虽然较轻，但仍与不施药对照有较大差异，也不能安全种植，高剂量处理药害仍很严重。     

The response of onion couch (Arrhenatherum elatius ssp. bulbosum (Willd.) Schub. & Mart.) to glyphosate and other foliage-applied herbicides

Two experiments were conducted in which foliage-acting herbicides were applied to onion couch (Arrhenatherum elatius ssp. bulbosum). The first experiment used an artificially established population and investigated the effects of straw burning on the performance of autumn-applied treatments of glyphosate, aminotriazole and dalapon. Spring barley was sown without cultivation. The second experiment, conducted on a naturally occurring infestation, compared the performance of glyphosate applied at alternative autumn timings and a single application of flamprop-methyl made in the following spring, in the presence of a winter wheat crop. In the first experiment, assessments made after 15 months suggested that straw burning resulted in increased growth. In addition, herbicide performance appeared less effective after burning. In neither case were these differences significant. Glyphosate at 1·0 and 1·5 kg a.e./ha gave the highest levels of control of both shoots and bulbs, whereas control from aminotriazole at all doses (1·5, 3·0 and 4·5 kg a.i./ha) was poor. Dalapon (6·0, 12·0 and 18·0 kg a.i./ha) was intermediate in its effectiveness. In the second experiment, applications of glyphosate at 1·0 and 1·5 kg a.e./ha resulted in high levels of control of both shoots and bulbs with no significant difference between application dates. Flamprop-methyl at 0·52 kg a.i./ha reduced the number of shoots and bulbs by 55% and 58% respectively. All treatments significantly reduced the number of inflorescences present in July.     

Herbicide tolerant canola systems and their impact on winter crop rotations

Canola is widely grown in Australia to provide diversity within winter crop rotations. Its production is heavily reliant on triazine tolerant varieties but Clearfield^® and conventional varieties are also significant components of the industry. Glyphosate tolerant varieties have been introduced, thereby providing an alternative technology to be incorporated into the farming systems. To evaluate the potential impact of the relative herbicide technologies, a comparison of herbicide tolerant canola weed management systems was undertaken at Wagga Wagga, Australia over a 5-year rotation. Near isogenic lines of conventional, glyphosate tolerant and triazine tolerant varieties were evaluated for their abilities to control annual ryegrass (Lolium rigidum), the most challenging weed of temperate crops in Australia. Glyphosate tolerant and triazine tolerant canola achieved high levels of ryegrass control and attained higher yields than the conventional system. Glyphosate tolerant canola provided extra control of broadleaf weeds and also achieved better seed oil levels when compared with the other canola systems. There were positive flow-on weed management benefits for the remainder of the crop sequence from the weed control achieved in the initial crop, particularly following glyphosate tolerant canola. Subsequent control of volunteer canola in all treatments was readily achieved by using paraquat/diquat. The glyphosate tolerant weed management system was more profitable than the triazine tolerant system, although no allowance was made for technology costs as they were not known at the time of study. These outcomes demonstrate the potential value of herbicide tolerant canola to Australian farmers.     

Effects of propylene oxide doses on Cyperus and Belonolaimus control and nutrient absorption in tomato

Among the current methyl bromide alternatives under study, propylene oxide has shown potential to control soilborne diseases, nematodes, and weeds in polyethylene-mulched tomato (Lycopersicon esculentum Mill.). Therefore, field trials were conducted to determine the most effective propylene oxide doses against populations for the nematode Belonolaimus spp. and the weed Cyperus spp. and their effect on nutrient uptake. Propylene oxide doses were 0, 190, 380, 570, 760, and 950 L/ha. Data indicated that populations of Belonolaimus and Cyperus rapidly decreased with 570 L/ha of propylene oxide. Propylene oxide doses also affected foliar concentrations of phosphorus and potassium. There was a linear increase of phosphorus concentration as dose increased, whereas potassium concentration increased rapidly after 190 L/ha of propylene oxide. The highest tomato yields were obtained with application of 760 and 950 L/ha of propylene oxide.     

Response of wheat (Tritium aestivum) to low rates of glyphosate and glufosinate

Field studies were conducted in 2007 and 2008 at Hamilton Farms near Lonoke, Arkansas, USA to evaluate the potential impact of sub-lethal doses of glyphosate and glufosinate on winter wheat (Tritium aestivum). Three wheat cultivars were seeded, and glyphosate and glufosinate were applied at 1/10 and 1/20 times the labeled dose at the 3- to 4-leaf, panicle initiation, and boot stages. Both herbicides caused significant injury to wheat; however, wheat response to both herbicides was different. Glufosinate injury occurred rapidly and consisted of mainly leaf necrosis. Glyphosate injury to wheat was minimal and manifested in the form of leaf yellowing. Glyphosate caused shortened flag leaf when applied at PI and reduced canopy height when applied at boot. Injury was minimal to wheat across all treatments, with the highest level of injury being 34% following glufosinate applied at boot. Conversely, wheat was most sensitive to glyphosate at the 3- to 4-leaf stage. Flag leaf length was reduced the greatest by glyphosate at PI, whereas glufosinate reduced flag leaf length minimally. Even though glyphosate injury was lower than that of glufosinate, yield was reduced more by glyphosate. Wheat seed weight and germination were not affected by either herbicide. Wheat appears to be slightly more sensitive to glyphosate with respect to yield; however, it is slightly more sensitive to glufosinate with respect to foliar injury.     

Glyphosate Carryover in Seed Potato: Effects on Mother Crop and Daughter Tubers

Field studies were conducted in 2008 and 2009 in Aberdeen, ID, Ontario, OR, and Paterson, WA to determine the effect of simulated glyphosate drift on ‘Ranger Russet’ potato during the application year and the crop growing the next year from the daughter tubers. Glyphosate was applied at 8.5, 54, 107, 215, and 423 g ae ha^?1 which corresponds to 1/00, 1/16, 1/8, ¼, and 1/2 of the lowest recommended single-application rate for glyphosate-resistant corn and sugar beet of 846 g ha^–1. Glyphosate was applied when potato plants were at 10 to 15 cm tall (Early), or at stolon hooking (H), tuber initiation (TI), or during mid-bulking (MB). In general, the MB applications caused less visual foliar injury to the mother crop than earlier applications at ID or OR, and H applications at WA. Mother crop injury increased as glyphosate rate increased regardless of location, application timing, and rating date. U.S. No.1 and total tuber yields were usually related to the injury level resulting from glyphosate application timings and rates. Although injury to the mother crop from glyphosate applied at MB usually was the lowest compared to injury from other application timings, when daughter tubers from that timing were planted the following year, emergence, plant vigor, and yield was most detrimentally impacted compared with that of daughter tubers from other timing treatments. MB daughter tuber emergence was less than 30 % of the nontreated control tuber emergence while emergence of daughter tubers from the other treatments was 60 to 95 %. As rate of glyphosate applied to the mother crop increased, daughter tuber emergence decreased. When MB daughter tubers did emerge, plants were chlorotic and stunted as if the plants had been directly sprayed with glyphosate. Regardless of whether the daughter tubers had defects or not, results the following year were the same. Implications are that if a mother seed crop encounters glyphosate during bulking, injury may not even be noticeable on the foliage or the tubers, however, emergence, vigor, and yield of the crop growing the following year from the daughter tubers could be greatly impacted.     

Control of winter cereals in the spring with glyphosate

Poor survival of winter cereals due to winter conditions in Ontario can necessitate destruction of the stand in the spring to allow the subsequent seeding of an alternative crop. Winter cereals were seeded in the autumn of 2004 and 2005 at the Huron Research Station and at the University of Guelph Ridgetown Campus in Ontario to evaluate two formulations of glyphosate [potassium (K) vs diammonium (DA) salt] at different doses (225, 450, 675, 900, or 1350 g a.e. ha⁻¹) for the burn-off of soft white winter wheat (SWW), soft red winter wheat (SRW), hard red winter wheat (HRW) and autumn rye (AR) in either late April or early May. There was no difference between the glyphosate formulations for the control of winter cereals at 1, 2, 3, and 4 weeks after treatment (WAT). There was generally improved control with glyphosate applications made in early May compared to late April however results were not always statistically significant. Generally, control of winter cereals increased as the glyphosate dose was increased from 225 to 1350 g a.e. ha⁻¹. The minimum dose of glyphosate required for providing 90% or greater control of SWW, SRW, HRW, and AR was 675 g a.e. ha⁻¹ at 4 WAT. Glyphosate applied at 675 g a.e. ha⁻¹ caused a 98, 97, 98, and 99% reduction in shoot dry weight of SWW, SRW, HRW, and AR, respectively. Based on this study glyphosate (K or DA) applied in late April or early May can be used at doses as low as 675 g a.e. ha⁻¹ to adequately control SWW, SRW, HRW, and AR in the spring.     

Effects of sulfosulfuron soil residues on barley (Hordeum vulgare), sunflower (Helianthus annuus) and common vetch (vicia sativa)

The phytotoxicity and sensitivity of succeeding crops to the new sulfonylurea, sulfosulfuron, have been reported although there is insufficient data on the phytotoxic effect of field soil residues. Growth chamber bioassays were conducted to detect the presence of residues in soil samples previously treated with sulfosulfuron at the recommended rate and double rate (20 and 40 g a.i./ha) that could affect the succeeding crop. Soil samples were collected between 7 and 9 months after sulfosulfuron application in field selectivity assays at nine different locations in Northern and Central Spain. The bioassay test species were barley (Hordeum vulgare L.) ‘Graphic’, sunflower (Helianthus annuus L.) ‘Albasol’ and common vetch (Vicia sativa L.) ‘Neska’, typical crops grown in rotation with wheat (Triticum aestivum L.) in this area. Sulfosulfuron residues did not affect barley and common vetch, but inhibited shoot length, root length and root dry weight of sunflower seeded into some soils treated with the 2× rate (40 g a.i/ha) 9 months earlier.     

Glyphosate management strategies,weed diversity and soybean yield in Argentina

From their introduction in 1996, glyphosate resistant (GR) soybean cultivars have been rapidly adopted by farmers in Argentina and in other countries in the world. The high rate of adoption of this technology seems to be based on the simplicity of use provided by a single herbicide (glyphosate), its high efficacy to control many weeds and, the low costs of the technology relative to that used in conventional crops. During 2001–2002, 2002–2003 and 2003–2004 soybean growing seasons, field surveys and field experiments were performed with the aim of studying the effect of different glyphosate management strategies on the diversity of soybean weed communities, weed control, individual survival, fecundity and crop yield. In addition, the emergence pattern of three important weeds, Digitaria sanguinalis, Cyperus rotundus and Anoda cristata, was also studied. Both field surveys and field experiments were carried out on no-till soybean crops sown immediately after wheat or barley harvest (double cropped system). Experiments were set up in commercial soybean crops and consisted of different times of a single glyphosate application, two glyphosate applications and also the application of glyphosate plus a residual herbicide imazethapyr. A. cristata, D. sanguinalis, Stellaria media, Chenopodium album and Cyperus sp. were the most prevalent weeds recorded at pre-harvest of the soybean crops, showing regional constancy higher than 80% in both years. In three out of four field experiments, crop yield was not increased when glyphosate was applied twice compared with a single application of the herbicide. In addition there was a lower negative effect on weed species richness when glyphosate was applied once during the crop cycle than with two applications of glyphosate or glyphosate plus imazethapyr. D. sanguinalis escaped the glyphosate early treatment because of the long weed emergence period, while A. cristata and C. rotundus survived treatments due to their high individual tolerance. The results suggest that it is possible to manage glyphosate application to get high crop yield with a low impact on weed diversity, depending on the weed species and their abundance.     

Effects of chrysophanol, parietin, and nepodin of Rumex crispus on barley and cucumber powdery mildews

Three substances that show antifungal activity against Blumeria graminis f. sp. hordei were isolated from roots of Rumex crispus and identified as chrysophanol, parietin, and nepodin. The substances were tested for plant disease control activity in vivo against six plant pathogenic fungi. All specifically reduced the development of barley powdery mildew. The concentrations required for 50% disease control were 4.7 μg/ml for chrysophanol, 0.48 μg/ml for parietin, and 20 μg/ml for nepodin. These agents showed both curative and protective activity against barley powdery mildew. Chrysophanol (100 μg/ml) and nepodin (400 μg/ml) were more effective than the fungicides fenarimol (30 μg/ml) and polyoxin B (100 μg/ml), under glasshouse conditions, against cucumber powdery mildew, which is caused by Podosphaera xanthii. Parietin (30 and 10 μg/ml) reduced the development of cucumber powdery mildew as efficiently as fenarimol (30 μg/ml) and more effectively than polyoxin B (100 μg/ml).     

草甘膦对抗草甘膦大豆光合特性日变化的影响

在大田条件下,采用随机区组设计,研究了不同用量草甘膦对抗草甘膦大豆(RR1)光合特性日变化的影响。结果表明:(1)在未喷施草甘膦情况下,抗草甘膦大豆(RR1)的净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)日变化均呈单峰曲线,峰值分别出现在10∶00、12∶00和10∶00。RR1叶片叶绿素含量指数(CCI)的日变化呈先降低后升高趋势,14∶00左右最低。水分利用效率(WUE)和胞间CO2浓度(Ci)随着时间的推移均呈波浪式变化,WUE在6∶00、10∶00和16∶00有3个小峰,而Ci在6∶00最高。(2)喷施草甘膦后,RR1光合特性的日变化趋势总体与未喷药前相似。Pn和CCI随草甘膦用量的增加呈降低趋势;但当草甘膦用量大于4.48 L.hm-2时,CCI和Pn显著下降。12∶00以前(包含12∶00),除低用量(1.12 L.hm-2)的草甘膦促进RR1的Gs外,各用量抑制了RR1的Tr和Gs;而12∶00以后,草甘膦却促进了RR1的Tr和Gs。草甘膦增加了RR1的Ci,而大于2.24 L.hm-2的草甘膦却降低了RR1的WUE。表明光强和草甘膦会影响大豆的光合特性和叶绿素含量。     

Effect of antagonistic Fusarium spp. and of different commercial biofungicide formulations on Fusarium wilt of basil (Ocimum basilicum L.)

The ability of antagonistic Fusarium spp. to control Fusarium wilt of basil caused by F. oxysporum f. sp. basilici was tested under glasshouse conditions from 1994 to 1996. Fusarium oxysporum antagonistic strain 251/2, a protoplast fusion-derived hybrid coded FI-11 and F. moniliforme strains TF4 and TF4 RB were able to decrease (P = 0.05) the incidence of Fusarium wilt significantly. Biocontrol was consistent, especially when the antagonists were applied by mixing a talc preparation of propagules in the substrate (10⁵ CFU/ml of substrate) 1–2 weeks before sowing or at sowing. Seed coating with the biocontrol strains (10⁸ CFU/g of seed) did not generally provide sufficient disease containment, whereas the combination of substrate treatment and seed coating did not improve wilt control in comparison with substrate treatment alone. Two commercial formulations, based on antagonistic F. oxysporum strains and one containing the antagonistic strain K61 of Streptomyces griseoviridis, were generally not sufficiently effective at the tested dosages.     

Weed control and wheat (Triticum aestivum L.) yield under application of 2,4-D plus carfentrazone-ethyl and florasulam plus flumetsulam: Evaluation of the efficacy

Three field experiments were conducted at the research fields of Plant Protection Research Institute, Iran, at different locations in 2004–2005 to study the efficacy of different broadleaved herbicides to control weeds in wheat. Treatments were the full-season hand weeded and weed-infested controls, and post-emergence applications of florasulam plus flumetsulam at 8.75, 10.50, and 12.25 g a.i./ha, 2,4-D plus carfentrazone-ethyl at 210, 245, 280, and 490 g a.i./ha, bromoxynil plus MCPA at 75, 100, and 150 g a.i./ha, 2,4-D at 560, 720, and 1120 g a.i./ha, tribenuron methyl, and 2,4-D plus MCPA. Herbicides were applied at wheat tillering stage. Naturally occurring broadleaved weed populations were used in experiments. Results indicated that bromoxynil plus MCPA at 150 g a.i./ha, 2,4-D plus MCPA, and 2,4-D plus carfentrazone-ethyl at 490 g a.i./ha were the best options to control weeds. Bromoxynil plus MCPA at 150 g a.i./ha and 2,4-D plus MCPA also resulted in the highest wheat yield. Overall, it could be concluded that rotational application of bromoxynil plus MCPA at 150 g a.i./ha, 2,4-D plus MCPA, and 2,4-D plus carfentrazone-ethyl at 490 g a.i./ha would be the best option to achieve satisfactory weed control, high grain yield and prevention of evolution of herbicide-resistant weeds.     

Effect of herbicides and annual weeds on the yield and seed quality of lathyrus (Lathyrus sativus L.)

Field research conducted over six location years has indicated that lathyrus (Lathyrus sativus L.) is a poor competitor with annual weed species. Failure to control annual weeds reduced lathyrus seed yields by 54–95%, depending on the weed species present and their density. Weed competition also reduced thousand-seed weight. Preplant soil-incorporated applications of trifluralin and postemergence applications of sethoxydim controlled wild oat (Avena fatua L.) and green foxtail (Setaria viridis (L.) Beav.). Trifluralin failed to control wild mustard (Sinapis arvensis L.), an important broad-leaved weed in western Canada, but this weed was controlled by both preplant soil-incorporated and postemergence applications of metribuzin. Lathyrus exhibited excellent tolerance to these herbicides, which are currently registered for use on high-acreage crops in Canada.     

含草甘膦抗性标记的水稻核不育繁殖系载体构建与验证

【目的】使用草甘膦抗性基因替代潮霉素抗性基因，构建核不育繁殖系的转化载体，赋予繁殖系对除草剂草甘膦的抗性，规避现行政策对转基因作物中使用抗生素标记基因的限制。【方法】利用同源重组的方法，构建含草甘膦抗性基因Epsps^#、花粉致死基因ZmAA1、核不育恢复基因OsEat1和红色荧光基因DsRed2的水稻eat1核不育基因繁殖系载体pC3300-Epsps-AA-Eat-Red;采用农杆菌介导法转化水稻。【结果】通过转化籼稻品系9K19-5获得普通核不育繁殖系Eat9K的转化植株318个。表型鉴定表明，单拷贝转化体Eat9K-3的花粉有可育和不育2种类型，种子有具有和不具有荧光信号2种类型，具有荧光信号的种子在发芽期能耐受浓度至少为1 g/L的草甘膦。建立的四引物检测法能够区分野生型Eat1基因和核不育eat1基因。【结论】证明载体pC3300-Epsps-AA-Eat-Red有效，创制了具有除草剂抗性的普通核不育繁殖系新种质，建立了区分野生型Eat1基因和核不育eat1基因的四引物检测法。     

Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide

The effect of volatile oil from leaves of Eucalyptus citriodora against some plant species viz. Triticum aestivum, Zea mays, Raphanus sativus, Cassia occidentalis, Amaranthus viridis and Echinochloa crus-galli was investigated. In a laboratory bioassay seed germination of test plants was significantly reduced in response to the different concentrations of the eucalypt oil. Maximum germination inhibition was observed with A. viridis, whereas least effect was seen on R. sativus. Based on the germination response, dose-response curve was generated and LC₅₀ values were calculated. It was maximum for R. sativus whereas minimum for A. viridis. Further, seedling growth of the test plants and the chlorophyll content in the treated seedlings was significantly reduced at concentrations 0.12 and 0.3 mg/l. Not only the initial growth, but also the spray treatment on the 4-week-old mature plants of two weedy species viz. C. occidentalis and E. crus-galli adversely affected the chlorophyll content and cellular respiration, thereby indicating the adverse effect of eucalypt oil on the photosynthetic machinery and the energy metabolism of the target plants. Based on the study, it is concluded that volatile oil from E. citriodora is phytotoxic and could be utilized as bioherbicide for future weed management programmes.