The use of wild relatives in crop improvement: a survey of developments over the last 20 years

Hypersensitive, race specific genes primarily have been deployed to control powdery mildew (Blumeria graminis (DC) EO Speer f. sp. tritici) in wheat (Triticum aestivum L.); however, recent efforts have shifted to breeding for more durable resistance. Previously, three quantitative trait loci (QTL) for adult plant resistance (APR) to powdery mildew in the winter wheat cultivar Massey were identified in a Becker/Massey (BM) F _2:3 population. Fourteen new simple sequence repeat (SSR) markers were added to the pre-existing BM F _2:3 linkage maps near the QTL for APR on chromosomes 1BL (QPm.vt-1BL), 2AL (QPm.vt-2AL), and 2BL (QPm.vt-2BL). Genetic linkage maps comprised of 17 previously and newly mapped SSRs from the BM population on chromosomes 1BL, 2AL, and 2BL were constructed in a USG 3209/Jaypee (UJ) F _6:7 recombinant inbred line (RIL) confirmation population, wherein the APR resistance of USG 3209 was derived from Massey. Interval mapping analysis of mildew severity data collected in 2002 (F _5:6) and 2003 (F _6:7) field experiments with marker genotypic data obtained in 2003 (F _6:7) confirmed the presence of the three QTL governing APR to powdery mildew in the UJ RILs. The QTL QPm.vt-1BL, QPm.vt-2AL, and QPm.vt-2BL explained 12–13, 59–69, and 22–48% of the phenotypic variance for powdery mildew severity in the UJ confirmation populations, respectively, in two field experiments. The current study verified that the elite wheat cultivar USG 3209 possesses the same QTL for APR as its parent Massey.     

两个抗小麦白粉病新基因的遗传分析与染色体定位

YU25是从八倍体小偃麦TAI7047与小麦栽培品种川麦107杂交后代中选育出的对白粉病免疫的小麦育种新材料。以感白粉病小麦品种MY11与YU25杂交和回交的后代F₁、F₂、BC₁F₁和BC₂F₁为材料,采用四川省当前流行的小麦白粉病优势生理小种人工接种,对YU25的白粉病抗性进行了遗传分析。结果表明,YU25含有2对表现免疫反应和高抗反应的显性抗病基因,暂命名为PmE(免疫)和PmYU25(高抗)。用294对小麦微卫星引物和221个F₂植株,对这2个基因进行连锁分析,发现小麦微卫星标记Xgwm-297-7B与PmE基因的遗传距离为13.0 cM,而Xgwm-210-2D与PmYU25基因的遗传距离为16.6 cM,因此将PmE和PmYU25分别定位在7BS和2DL上。根据系谱和基因位点分析,推断PmE和PmYU25均为起源于中间偃麦草、不同于已知的抗小麦白粉病基因的2个新基因。小麦育种新材料YU25含有可能来源于小麦-中间偃麦草的染色体多重易位,其细胞学基础和在实际育种中的应用值得进一步研究。     

小麦白粉病成株抗性和抗倒伏性及穗下节长度的QTL定位

由小麦品种花培3号和豫麦57杂交获得了DH群体168个株系, 利用305个SSR标记对白粉病成株抗性、抗倒伏性和穗下节长度进行了QTL定位研究。DH群体及两亲本于2005年和2006年种植于山东泰安, 2006年种于安徽宿州。利用基于混合线性模型的QTLNetwork 2.0软件, 共检测到12个加性效应位点和10对上位效应位点。在4D染色体上控制白粉病成株抗性的qApr4D, 贡献率为20.0%, 在各环境中稳定表达, 其抗病等位基因来源于抗病亲本豫麦57; 在7D染色体上控制小麦穗下节长度的qIlbs7D, 贡献率为12.9%, 在各环境中稳定表达。加性效应和上位效应对小麦白粉病成株抗性、抗倒伏性和穗下节长度的遗传起重要作用, 并且基因与环境常常具有互作效应。以上两个QTL可分别用于小麦白粉病成株抗性和穗下节长度的分子标记辅助选择。     

A new powdery mildew resistance gene: Introgression from wild emmer into common wheat and RFLP-based mapping

An Israeli accession (TTD140) of wild emmer, Triticum turgidum var. dicoccoides, was found resistant to several races of powdery mildew. Inoculation of the chromosome-arm substitution lines (CASLs) of TTD140, in the background of the Israeli common wheat cultivar ‘Bethlehem’ (BL), with five isolates of powdery mildew revealed that only the line carrying the short arm of chromosome 2B of wild emmer (CASL 2BS) exhibited complete resistance to four of the five isolates. To map and tag the powdery mildew resistance gene, 41 recombinant substitution lines, derived from a cross between BL and CASL 2BS, were used to construct a linkage map at the gene region. The map, which encompasses 69.5 cM of the distal region of chromosome arm 2BS, contains six RFLP markers, a morphological marker (glaucousness inhibitor, W1 ^I), and the powdery mildew resistance gene. Segregation ratios for resistance in F₂ of BL × CASL 2BS and in the recombinant lines, combined with the susceptability of F₁ progeny to all tested isolates, indicate that resistance is controlled by a single recessive allele. This alleleco-segregated with a polymorphic locus detected by the DNA marker Xwg516, 49.4 cM from the terminal marker Xcdo456. The new powdery mildew resistance gene was designated Pm26. This revised version was published online in July 2006 with corrections to the Cover Date.     

普通小麦品种“豫麦66”抗白粉病基因的鉴定与分子标记

豫麦66是对小麦白粉病(Blumeria graminis f. sp. tritici)具有良好抗性的小黑麦后代品种。本试验通过抗病鉴定与遗传分析, 明确了豫麦66携带1个抗白粉病显性单基因, 暂命名为PmYm66。采用2个以豫麦66为抗病亲本的杂交组合(豫麦66/铭贤169和豫麦66/ND3509)F2代抗、感病分离群体和F3代家系, 利用集群分离分析法(BSA)找到了与PmYm66连锁的分子标记XKsum193、EST48、EST83和EST84, 抗病基因和分子标记的顺序为EST48—EST83 (EST84)—Xksum193—PmYm66, 并通过中国春缺体-四体、双端体和缺失系将PmYm66基因及其连锁的分子标记定位在2AL染色体臂末端。多小种鉴定结果表明PmYm66(豫麦66)与2AL染色体臂上已有的Pm4a(Khapli/8Cc)和Pm4b(Armada)基因存在致病反应型差异。     

Mapping QTL involved in adult plant resistance to powdery mildew in the winter wheat line RE714 in two susceptible genetic backgrounds

The objective was to study the genetic basis of adult plant resistance to powdery mildew of the winter wheat line RE714 by quantitative trait loci (QTL) analysis and to investigate the stability of the QTL detected in two different genetic backgrounds. Two DH populations from the crosses between RE714 and the susceptible parents ‘Festin’ and ‘Hardi’ were used. Reaction of the DH lines to powdery mildew was assessed in different environments in Belgium under natural disease infection. Considering both populations and according to the environment tested, one to seven QTL were detected. Among them, residual effects of the race‐specific resistance genes Pm4b and MIRE were found. Two major QTL were very stable (on chromosome 5D and at the MIRE locus), since they were detected in both populations and over all environments tested. The QTL detected varied according to the susceptible parent used, and a residual effect at the Pm4b gene was not observed with the genetic background of ‘Hardi’.     

普通小麦白粉病成株抗性的QTL分析

以抗白粉病的日本小麦品种Fukuho-komugi和以色列小麦Oligoculm杂交F₁的DH（doubled haploid）群体107个系为材料，利用313个SSR标记和37个RFLP标记，对Fukuho-komugi和Oligoculm的白粉病成株抗性进行QTL分析。试验材料于2003－2004年度种植在北京、2003－2004和2004－2005年度种植在安阳，调查白粉病发病情况。构建了由350个位点组成的遗传连锁图，覆盖小麦21个连锁群，全长3 101 cM。采用复合区间作图法进行白粉病成株抗性QTL分析，在1A、2B、4B和7D上发现4个抗白粉病QTL，分别解释13.6%、6.6%、8.9%和12.7%的表型变异。抗白粉病基因及其紧密连锁分子标记的发掘，将为小麦抗白粉病育种的分子标记辅助选择提供理论和技术支持。     

Identification of quantitative trait loci for powdery mildew resistance in highly resistant cucumber (Cucumis sativus L.) using ddRAD-seq analysis

Powdery mildew, caused by Podosphaera xanthii (syn. Sphaerotheca fuliginea ex Fr. Poll.), is one of the most economically important foliar diseases in cucumber (Cucumis sativus L.). Cucumber parental line ‘Kyuri Chukanbohon Nou 5 Go’, developed from weedy cucumber line CS-PMR1, is highly resistant to powdery mildew and is promising breeding material. We performed quantitative trait locus (QTL) analysis using double-digest restriction-site-associated DNA sequencing (ddRAD-Seq) in a population from a cross between ‘Kyuri Chukanbohon Nou 5 Go’ and the Japanese native cultivar ‘Kaga-aonaga-fushinari’, which is susceptible to powdery mildew. The resistance of the population and its parents was evaluated using leaf disc assays and image analysis. We detected one major QTL on Chr. 5 that was effective at both 20°C and 25°C and one minor QTL on Chr. 1 effective at 20°C. We detected two additional QTLs in subpopulation: one on Chr. 3 effective at 20°C and one on Chr. 5 effective at both 20°C and 25°C in a position different from the major QTL. The resistance alleles at all four QTLs were contributed by ‘Kyuri Chukanbohon Nou 5 Go’. The results of this study can be used to develop practical DNA markers tightly linked to genes for powdery mildew resistance.     

河南小麦新育成品种(系)白粉病抗性鉴定与分子标记检测

利用华北地区流行的白粉菌菌株E09和E20,分别对河南省小麦新品种(系)区域和预备试验参试材料908份(2009—2013年度)和412份(2009—2012年度)进行苗期白粉病抗性鉴定,同时利用与Pm2、Pm4a、Pm8和Pm21基因连锁的分子标记检测相关抗病基因的分布。结果显示,抗E09的材料占21.9%(199/908),抗E20的材料占9.5%(39/412),同时抗E09和E20的材料仅占3.6%(15/412)。在908份供试材料中,580份含有1BL/1RS,占63.9%,含Pm8或新的1RS来源抗白粉病基因;另有2份材料含6AL/6VS来源广谱抗白粉病基因Pm21,8份可能携带Pm2,2份可能含有Pm4a;有6份材料可能含有多个抗白粉病基因。表明河南省近年育成的小麦新品种(系)依然含有对我国白粉菌菌系有效的抗白粉病基因,但抗源遗传基础较窄,部分已经或正在丧失抗性,应加快引进和利用新的多样化抗病基因资源。     

Detection of downy and powdery mildew resistance QTL in a ‘Regent’ × ‘RedGlobe’ population

One hundred and eighty six F₁ plants from a ‘Regent’ × ‘RedGlobe’ cross were used to generate a partial linkage map with 139 microsatellite markers spanning all 19 chromosomes. Phenotypic scores for downy mildew, taken over two years, confirmed a major resistance QTL (Rpv3) against downy mildew in the interval VVIN16-cjvh to UDV108 on chromosome 18 of ‘Regent’. This locus explained up to 62 % of the phenotypic variance observed. Additionally a putative minor downy mildew resistance locus was observed on chromosome 1 in one season. A major resistance locus against powdery mildew (Ren3) was also identified on chromosome 15 of ‘Regent’ in the interval UDV116 to VChr15CenGen06. This study established the efficacy of and validated the ‘Regent’-derived downy and powdery mildew major resistance genes/QTL under South African conditions. Closely linked SSR markers for marker-assisted selection and gene pyramiding strategies were identified.     

簇毛麦6VS上4个新分子标记的鉴定及与抗白粉病基因Pm21的连锁分析

Pm21具有强抗白粉病特性，位于簇毛麦6V染色体短臂（6VS）上。染色体分析和白粉病抗性检测表明，Pm21在受体小麦内是稳定遗传的。筛选与Pm21相连锁分子标记，在小麦抗白粉病辅助选择育种上有重要意义。利用RAPD和AFLP分子标记方法，对簇毛麦6V染色体和含有6V的小麦-簇毛麦代换系、6VS的易位系6AL/6VS、6DL/6VS进行分子标记筛选，以分析位于6VS上的分子标记及其与Pm21的关系。RAPD检测表明，OPK08910特异片段存在于含簇毛麦6V染色体的代换系（6A/6V）、易位系（6AL/6VS，6DL/6VS）和簇毛麦中。AFLP检测显示，PstⅠ+AGG / MseⅠ+CAC、PstⅠ+ACC / MseⅠ+CCT和PstⅠ+ AAG / MseⅠ+CGC 共3对引物分别可以在6A/6V、6AL/6VS、6DL/6VS和VV中扩增出264 bp、218 bp和232 bp特异带，并与抗白粉病基因Pm21共分离，因此，上述来源于6VS上的4个新的分子标记，可作为源自簇毛麦Pm21基因的选择标记，用于小麦抗病育种。     

QTL mapping of adult‐plant resistance to stripe rust in a ‘Lumai 21 × Jingshuang 16’ wheat population

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a devastating fungal disease in common wheat (Triticum aestivum L.) worldwide. Chinese wheat cultivars ‘Lumai 21’ and ‘Jingshuang 16’ show moderate levels of adult‐plant resistance (APR) to stripe rust in the field, and they showed a mean maximum disease severity (MDS) ranging from 24 to 56.7% and 26 to 59%, respectively, across different environments. The aim of this study was to identify quantitative trait loci (QTL) for resistance to stripe rust in an F₃ population of 199 lines derived from ‘Lumai 21’ × ‘Jingshuang 16’. The F₃ lines were evaluated for MDS in Qingshui, Gansu province, and Chengdu, Sichuan province, in the 2009–2010 and 2010–2011 cropping seasons. Five QTL for APR were detected on chromosomes 2B (2 QTL), 2DS, 4DL and 5DS based on mean MDS in each environment and averaged values from all three environments. These QTL were designated QYr.caas‐2BS.2, QYr.caas‐2BL.2, QYr.caas‐2DS.2, QYr.caas‐4DL.2 and QYr.caas‐5DS, respectively. QYr.caas‐2DS.2 and QYr.caas‐5DS were detected in all three environments, explaining 2.3–18.2% and 5.1–18.0% of the phenotypic variance, respectively. In addition, QYr.caas‐2BS.2 and QYr.caas‐2BL.2 colocated with QTL for powdery mildew resistance reported in a previous study. These APR genes and their linked molecular markers are potentially useful for improving stripe rust and powdery mildew resistances in wheat breeding.     

Potential for effective marker-assisted selection of three quantitative trait loci conferring adult plant resistance to powdery mildew in elite wheat breeding populations

Three quantitative trait loci (QTL) associated with adult plant resistance (APR) to powdery mildew (Blumeria graminis) in wheat (Triticum aestivum) cultivar ‘Massey’ were mapped in a previous study. The three QTL were located on chromosomes 2A, 2B and 1B, and explained 50% of the total phenotypic variation. A 293 recombinant inbred line (RIL) breeding population (UJ) derived from the cross of ‘USG 3209’, a derivative of ‘Massey’, and ‘Jaypee’ was used to evaluate the potential effectiveness of marker‐assisted selection (MAS) for APR. Powdery mildew severities of the 293 UJ RILs were evaluated in 2002 (F_{5 : 6}) and 2003 (F_{6 : 7}) under natural disease pressure in the field. The 293 RILs were also evaluated for disease severity in a 2004 (F_{7 : 8}) greenhouse experiment using a composite of five different isolates of B. graminis. Selection of RILs possessing the QTL on chromosome 2A, and to a lesser extent, the one on chromosome 1B was effective in identifying powdery mildew resistance in both greenhouse and field experiments. Overall, selecting RILs with QTL on chromosomes 2A and 2B was most successful in identifying highly resistant RILs, which had mean mildew severities of 4.4% and 3.2% in 2002 and 2003 field experiments, respectively. Breeders implementing MAS programs for APR to powdery mildew via selection of RILs containing the two QTL on chromosomes 2A and 2B likely will obtain RILs having high levels of resistance in the field, however combining all three QTL may ensure greater durability.     

小麦新种质N9628-2抗白粉病基因的SSR分析

以抗白粉病的波斯小麦-小伞山羊草双二倍体Am9为母本, 与高感白粉病的普通小麦品种陕160杂交, 并用陕160回交一次, 从其后代中选育的普通小麦种质N9628-2对陕西省关中地区白粉病流行小种关中4号表现免疫。为了明确N9628-2所携带抗性基因的遗传方式及与抗性基因连锁的分子标记, 对该种质的抗白粉病基因进行了遗传分析和SSR标记分析。用高感白粉病品种陕160、陕优225与N9628-2杂交, F₁代对白粉病均表现高抗, F2代抗感分离比例均符合3∶1, 表明N9628-2的白粉病抗性由1对显性基因控制。通过208对SSR引物对陕160 ´ N9628-2 F2代抗感分离群体的142个单株的检测, 发现位于6A上的SSR位点Xwmc553和Xwmc684在双亲和抗、感池间有特异性, 并与抗性基因连锁, 遗传距离分别是10.99和7.43 cM, 表明抗病基因可能位于6A染色体上。 用中国春部分第6同源群的缺体-四体系和双端体系进行验证, 进一步将抗性基因定位在6AS。用连锁的SSR标记和相关亲本分析表明, 该抗病基因可能来源于小伞山羊草Y39, 它不同于已有抗白粉病基因, 可能是一个新基因。     

Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer

Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of theworld. A powdery mildew resistance gene, originating from wild emmerwheat (Triticum dicoccoides) accession `C20', from Rosh Pinna, Israel,was successfully transferred to hexaploid wheat through crossing andbackcrossing. Genetic analysis indicated that a single dominant genecontrols the powdery mildew resistance at the seedling stage. SegregatingBC₁F₂ progenies of the cross 87-1/C20//2*8866 wereused for bulked segregant analysis (BSA). The PCR approach was used togenerate polymorphic DNA fragments between the resistant and susceptibleDNA pools by use of 10-mer random primers, STS primers, and wheatmicrosatellite primers. Three markers, Xgwm159/430,Xgwm159/460, and Xgwm159/500, were found to be linked tothe resistance gene. After evaluating the polymorphic markers in twosegregating populations, the distance between the markers and the mildewresistance gene was estimated to be 5–6 cM. By means of ChineseSpring nullisomic-tetrasomics and ditelosomics, the polymorphic markersand the resistance gene were assigned to chromosome arm 5BS and werephysically mapped on the gene rich regions of fragment length (FL) 0.41–0.43 by Chinese Spring deletion lines. As no powdery mildew resistancegene has been reported on chromosome arm 5BS, the mildew resistancegene originating from C20 should be a new gene and is designated Pm30.     

Molecular mapping of powdery mildew resistance genes in wheat: A review

Powdery mildew, caused by Blumeria graminis f. sp. tritici (syn. Erysiphe graminis f. sp. tritici), is one of the most important diseases of common wheat (Triticum aestivum L.) worldwide. Molecular mapping and cloning of genes for resistance to powdery mildew in hexaploid wheat will facilitate the study of molecular mechanisms underlying resistance to powdery mildew diseases and help understand the structure and function of powdery mildew resistance genes, and permit marker-assisted selection in breeding programs. So far, 48 genes/alleles for resistance to powdery mildew at 32 loci have been identified and located on 16 different chromosomes, of which 21 resistance genes/alleles have been tagged by restriction fragment length polymorphisms (RFLPs), random-amplified polymorphic DNAs (RAPDs), amplified fragment length polymorphisms (AFLPs), sequence characterized amplified regions (SCARs), sequence-tagged sites (STS) or simple sequence repeats (SSRs). Several quantitative trait loci (QTLs) for adult plant resistance (APR) to powdery mildew have been associated with molecular markers. The detailed information on chromosomal location and molecular mapping of these genes has been reviewed. Isolation of powdery mildew resistance genes and development of valid molecular markers for pyramiding resistance genes in breeding programs is also discussed.     

Exploring wheat landraces for rust resistance using a single marker scan

Marker-trait associations identified in diverse germplasm can be exploited in crop improvement programs. An attempt to establish such associations was made by evaluating 205 wheat landraces for stripe rust, leaf rust and stem rust responses in the field over three crop seasons. Diversity arrays technology was used to genotype the landraces and associations were identified using a single-marker scan. Sixty-eight markers were significantly associated with rust resistance. Several significantly associated loci coincided with the presence of known major genes or QTL for rust resistance. In contrast, many marker-rust response associations identified in this analysis for each of the three rust diseases uncovered new loci. Dual associations; stripe rust-leaf rust (1AL, 2BS, 2BL, 3DL, 5BS, 6BS and 7DL), leaf rust-stem rust (5BL) and stripe rust-stem rust (4BL and 6AS) resistance were also observed. These associations could enable a cost-effective targeted mapping of dual rust resistance. Some marker-trait associations identified in this study have been validated through genetic analyses and formal naming of resistance loci.     

野生二粒小麦导入普通小麦的抗白粉病基因MlWE29分子标记定位

小麦白粉病是严重影响小麦生产的重要病害之一,培育和应用抗病品种是有效控制和减少病害的最经济有效的方法。野生二粒小麦是硬粒小麦和普通小麦的四倍体野生祖先种,是小麦抗病性遗传改良的重要基因资源。本研究利用来自以色列的野生二粒小麦WE29与普通小麦杂交,再用普通小麦连续回交和自交,育成高抗白粉病(Blumeria graminis f. sp. tritici)小麦新品系3D258(系谱为燕大1817/WE29//5*87-1, BC₄F₆)。将3D258和高感小麦白粉病的普通小麦品种薛早配制杂交组合,对其F₁、F₂代分离群体和F₃代家系进行白粉病抗性鉴定和遗传分析。结果表明3D258携带抗白粉病显性单基因,暂命名为MlWE29。利用集群分离分析法(BSA)和分子标记分析,发现6个SSR标记(Xgwm335、Xgwm213、Xgwm639、Xwmc415、Xwmc289和Xwmc75)和5个EST-STS标记(BE494426、BE442763、CD452476、BE445282和BE407068)与抗白粉病基因MlWE29连锁。利用中国春缺体-四体系、双端体系和缺失系将抗白粉病基因MlWE29标记物理定位于5BL染色体的0.59–0.79区域。这一普通小麦抗白粉病种质资源的创制及其连锁分子标记的建立为小麦抗病基因分子标记辅助选择、基因积聚和分子育种提供了新的物质基础。     

野生二粒小麦导入普通小麦的抗白粉病基因MlWE29分子标记定位

小麦白粉病是严重影响小麦生产的重要病害之一,培育和应用抗病品种是有效控制和减少病害的最经济有效的方法。野生二粒小麦是硬粒小麦和普通小麦的四倍体野生祖先种,是小麦抗病性遗传改良的重要基因资源。本研究利用来自以色列的野生二粒小麦WE29与普通小麦杂交,再用普通小麦连续回交和自交,育成高抗白粉病(Blumeria graminis f. sp. tritici)小麦新品系3D258(系谱为燕大1817/WE29//5*87-1, BC₄F₆)。将3D258和高感小麦白粉病的普通小麦品种薛早配制杂交组合,对其F₁、F₂代分离群体和F₃代家系进行白粉病抗性鉴定和遗传分析。结果表明3D258携带抗白粉病显性单基因,暂命名为MlWE29。利用集群分离分析法(BSA)和分子标记分析,发现6个SSR标记(Xgwm335、Xgwm213、Xgwm639、Xwmc415、Xwmc289和Xwmc75)和5个EST-STS标记(BE494426、BE442763、CD452476、BE445282和BE407068)与抗白粉病基因MlWE29连锁。利用中国春缺体-四体系、双端体系和缺失系将抗白粉病基因MlWE29标记物理定位于5BL染色体的0.59–0.79区域。这一普通小麦抗白粉病种质资源的创制及其连锁分子标记的建立为小麦抗病基因分子标记辅助选择、基因积聚和分子育种提供了新的物质基础。     

Genetic diversity of resistance genes controlling fusarium head blight with simple sequence repeat markers in thirty-six wheat accessions from east asian origin

Summary Fusarium head blight (FHB) is a serious disease of wheat worldwide that may cause substantial yield and quality losses. Breeding for FHB-resistant cultivars is the most cost-effective approach to control FHB. The objective of the present study was to determine the relationship of resistance between new resistant sources and Sumai 3 using five simple sequence repeat (SSR) markers closely linked to the major QTL for FHB resistance on chromosome arms 3BS and 6BS. All five SSR markers were highly polymorphic between Sumai 3 (and its derivatives) and susceptible Canadian wheat lines. Most of the Sumai 3-derived Chinese wheat accessions and three Canadian FHB-resistant lines had all the Sumai 3 SSR marker alleles on chromosome arms 3BS and 6BS. The Chinese landrace Wangshuibai and two Japanese accessions Nobeokabozu and Nyu Bai had the same banding patterns as Sumai 3 for all five SSR marker alleles, and another Chinese landrace Fangshanmai had three of the five SSR markers in common with Sumai 3, and therefore most likely carries the same QTL as Sumai 3 on 3BS and 6BS. The Brazilian cultivar Frontana had no alleles in common with Sumai 3 on either QTL, and the Chinese landrace Hongheshang had only one of the five SSR markers in common with Sumai 3, therefore likely carrying resistance genes different from Sumai 3. The Italian cultivar Funo is not the donor of either the 3BS QTL or 6BS QTL. All five SSR seem to be effective candidates for marker-assisted selection to increase the level of resistance to FHB in wheat breeding programs.