2. IGF1 mRNA expression in breast muscle in Taihu geese was significantly higher than that in Wanxi geese and the MSTN mRNA level in leg muscle in Taihu geese was significantly higher than that in Wanxi geese.
3. There was no significant difference in breast muscle MSTN or leg muscle IGF1 mRNA expression between the two breeds.
4. Within the same breed, the IGF1 mRNA expression in leg muscle of male geese was significantly higher than that in female geese, and MSTN mRNA expression in leg muscle was significantly higher than that in breast muscle.
5. There was no difference in the IGF1 mRNA expression between tissues.
6. There was a positive correlation between IGF1 mRNA and MSTN mRNA and a negative correlation between IGF1 mRNA expression of breast muscle and leg muscle ratio.
7. In Wanxi geese, MSTN mRNA expression in leg muscle was negatively associated with body weight and leg muscle weight. 相似文献
2. A total of 36 ducks (12 males and 24 females) were obtained from local farmers and tissue samples of gut tissues (duodenum, jejunum, ileum and caecum), lymphoid organs (spleen and bursa) and lungs were collected in RNAlater solution followed by RNA extraction.
3. After normalisation to β-actin (endogenous control) qPCR analysis identified a significant effect of age, sex and rearing system on TLR7 expression in the ducks.
4. A significant up-regulation of TLR7 expression was observed in lungs, duodenum, jejunum, ileum and caecum of sexually mature (45 wk) compared with that of immature ducks (16 wk). Among sexes, male ducks had significantly higher TLR7 expression than female ducks.
5. Age and sex interactions were significant in lungs, duodenum, jejunum and caecum. Ducks reared in an extensive housing system showed significantly higher TLR7 expression in bursa, lungs, duodenum, ileum and caecum compared to intensively reared ducks. There were no effects of age, sex and rearing systems on TLR7 expression in the spleen.
6. The heterophil-to-lymphocyte ratio and serum corticosterone were higher in ducks reared on an intensive system compared with ducks from an extensive rearing system. 相似文献
2. In a completely randomised design, different probiotic strains were injected into the amniotic fluid of the 480 live embryos (d 18 of incubation), with 4 treatments and 5 replicates. Ileal MUC2 gene expression, microbial profile, growth performance and immune response were determined.
3. Injection of probiotic strains, especially B. subtilis, had significant effect on expression of the MUC2 on d 21 of incubation and d 3 post-hatch, but not on d 19 of incubation.
4. Injection of the probiotic strains decreased significantly the Escherichia coli population and increased the lactic acid bacteria population during the first week post-hatch.
5. Inoculation of probiotics had no significant effect on antibody titres against Newcastle disease virus, antibody titres against sheep red blood cell and cell-mediated immune response of chickens compared to control.
6. In ovo injection of the probiotic strains had no significant effect on growth performance of broiler chickens.
7. It was concluded that injection of probiotic bacteria especially B. subtilis into the amniotic fluid has a beneficial effect on ileal MUC2 gene expression and bacteria population during the first week post-hatch, but has no effect on growth performance and immune response in broiler chickens. 相似文献
2. A total of 160 Hy-Line Brown layers (45 weeks old) were randomly assigned into 4 treatments on the basis of laying performance. Each treatment had 4 replicates with 10 birds each.
3. Dietary treatments were basal diet supplemented with 0 (control), 2.5, 5.0 and 7.5 g/kg diet G. procumbens during 56-d feeding period.
4. Serum (d 21, 42 and 56) and egg yolk (d 28, 42 and 56) cholesterol and triglycerides concentrations were linearly reduced with increasing dietary concentrations of G. procumbens.
5. Increasing dietary concentrations of G. procumbens linearly reduced the excreta total anaerobic bacteria (d 28), Clostridium sp. and Escherichia coli (d 28 and 56) populations.
6. Overall egg production and egg mass were linearly increased, and overall feed efficiency was linearly improved with increase in dietary G. procumbens.
7. Dietary increasing concentrations of G. procumbens linearly improved egg yolk colour (d 28 and 56) and breaking strength of eggs (d 56).
8. The results obtained in the present experiment indicate that dietary supplementation with G. procumbens could reduce the egg yolk cholesterol, suppresses harmful excreta microflora and improves layers performance. 相似文献
2. Dry plucking increased weight loss due to plucking and eviscerating, and reduced chilled carcass yield.
3. Dry eviscerating increased carcass water uptake during chilling.
4. Pre‐chilling with running water increased carcass water uptake compared with chilling in ice and water alone.
5. Water uptake during chilling appeared to be increased by longer eviscerating time. 相似文献
2. The Sinai produced significantly fewer, smaller eggs, resulting in lower egg mass output (g/bird d), than the Leghorn and the crossbreds.
3. Egg weight of both crossbreds was intermediate between Sinai and Leghorn but laying rate was closer to that of the Leghorn.
4. Differences in egg mass output apparent at 8 to 10 months of age decreased considerably with age.
5. These findings suggest that selection or crossbreeding of the Sinai might improve productive performance while maintaining improved eggshell quality. 相似文献
2. In 4 consecutive trials, 4752 hens were recorded for keel bone status. Evaluation of plumage condition was made for 1440 hens and bone breaking strength was recorded for 1200 hens. A total of 4962 eggs were analysed for internal and external egg quality traits. Analyses involved 30 small group compartments per trial.
3. The layer line had a much more pronounced influence on humerus breaking strength than on tibia breaking strength.
4. Plumage condition, particularly on the neck, was positively correlated with humerus breaking strength in both layer lines.
5. An average of 34% of LB and 23% of LSL hens showed keel bone deformities, with higher proportions of slight deformities.
6. Slight keel bone deformities, rather than moderate to severe, increased significantly during the laying period.
7. Stocking density had no influence on bone breaking strength, keel bone status and egg quality traits.
8. LB layers had a 1.4-fold higher humerus, but only a 1.06-fold higher tibia breaking strength compared to LSL layers.
9. Tibia breaking strength was significantly affected by the interaction of group size and layer line. LSL layers in small groups had lower tibia breaking strengths than those of the large groups. 相似文献
2. A circadian system was postulated to control the restriction of ovulation to an 8‐h period of the day under conventional 14 h light: 10 h dark regimes.
3. The final phase of follicular maturation was postulated to commence after ovulation of the preceding ovum in the hierarchy.
4. Ovulation was postulated to occur when a mature follicle was present in the ovary during the appropriate phase of the circadian‐linked system.
5. The predicted times of oviposition were within the standard error of the observed times of oviposition under 21‐, 24‐ and 28‐h photoschedules.
6. It was concluded that this hypothesis for the control of the ovulatory cycle of the hen is consistent with current knowledge. 相似文献
2. Developmental changes of gene expression of xanthine oxidoreductase (XOR), XO activity and UA content of liver and kidney tissues in both broiler and layer chicken embryos were examined during incubation d 14–21.
3. In both strains, hepatic XOR gene expression peaked on d 21 while renal XOR gene expression did not change.
4. The XO activity was higher in kidney than liver in both strains. Hepatic XO activity of both strains peaked on d 18 and thereafter was decreased on d 21. Renal XO activity peaked on d 18 and from then on did not show any significant changes until d 21 in both strains.
5. The UA content was higher in kidney vs. liver in both strains. The hepatic and renal UA values of the both strains increased significantly from d 14 to d 21.
6. The present results showed dissimilar behaviour of XOR gene expression, XO activity and UA content of liver and kidney tissues in both broiler and layer chicken embryos. 相似文献
2. Restricted birds were lighter throughout the experiment.
3. Relative adrenal weight tended to be greater in restricted birds but the difference decreased with time.
4. There was no depletion of adrenal cholesterol: from week 5 there was a significantly greater amount in the adrenals of restricted birds.
5. After 1 week of restriction plasma corticosterone concentration was 73% greater than in controls. It decreased progressively, falling within the normal range at 5 weeks.
6. Restricted birds were hypoglycaemic from weeks 2 to 7 and hyper‐lipacidaemic throughout. A negative correlation between plasma glucose and free fatty acids was found. 相似文献
2. Chicken HSP70 sequence (GenBank: AY143693) was used as a template to design a primer pair to amplify partial duck HSP70 gene. Primers were subsequently designed with the duck HSP70 gene as a template to amplify the complete duck HSP70 sequence.
3. Twelve commercial Sanshui White ducklings were subjected to a heat stress experiment. Tissue samples were collected for RNA extraction and real-time PCR to analyse the expression mechanism of duck HSP70.
4. A DNA pool was constructed from three different species for single nucleotide polymorphism (SNP) screening. The genotypes of the identified SNPs were detected in 145 Sanshui White ducklings.
5. Duck HSP70 gene was identified and characterised (GenBank: EU678246) and shown to contain no introns. Fifteen variations were identified within the open reading frame. Quantitative real-time PCR results showed that the expression of duck HSP70 gene was tissue specific and the highest expression level was seen in pectoral muscle. 相似文献
2. Eighty broilers were randomly divided into two groups. Birds in the control group were fed on maize–soybean-based diets. Birds in the treatment group were provided the same diet supplemented with 2 g/kg BA encapsulated in a vegetable oil matrix.
3. At the end of the trial (d 35), pH, bacterial composition and metabolites were determined in the crop, jejunum, ileum and caecum.
4. Growth performance variables and pH were not significantly different.
5. BA concentration decreased rapidly in the proximal gut. However, the treatment diet showed higher BA in the crop, jejunum, ileum and caecum.
6. Total lactate in the crop and D-lactate in the jejunum was higher in the BA treated group. Caecal total and branched chain fatty acids were decreased due to the treatment.
7. Lactobacilli populations were significantly altered by BA supplementation. A trend for increased lactobacilli was observed in the crop, while it became significant in the jejunum and ileum. Lactobacillus species responded differently to the treatment. Four of 5 measured Lactobacillus species, particularly in the ileum, followed the course observed for total lactobacilli; only Lactobacillus salivarius was not modified.
8. Correlation analysis showed that BA modified the intestinal microbiota. Lactobacilli correlated negatively to all studied clostridial clusters and enterobacteria. Clostridial clusters IV and XIVa were significantly increased in the jejunum, whereas only clostridial cluster XIVa was increased in the caecum.
9. Encapsulated BA modified the intestinal microbiota which can lead to the conclusion, that the main beneficial mode of action of BA in the gut appears to be the enhancement of lactic acid bacteria, which in turn may act as a vanguard against pathogens. 相似文献
2. The different varieties of wheat investigated contained different proportions of water‐soluble to water‐insoluble hemicelluloses.
3. By comparison of the arabinose to xylose ratios it was concluded that water‐soluble and insoluble arabinoxylans of wheat were branched to the same degree.
4. Starch from six wheat varieties grown in the U.K. was very well digested by adult cockerels and true metabolisable energy values of the wheats were high.
5. Small amounts of cell wall carbohydrates were digested by adult cockerels. 相似文献
2. Single pre‐ovulatory peaks of plasma LH, androgen and progesterone were observed which took 8, 8 and 12 h respectively, to increase and return to base‐line values. The concentration of plasma prolactin tended to be elevated between 6 h before and 6 h after the LH peak with the maximum values occurring after the peak.
3. The changes in the concentrations of plasma LH and progesterone were 3‐ and 7‐fold respectively while 2‐fold changes were observed in the concentrations of plasma androgen and prolactin.
4. The pre‐ovulatory concentration of plasma progesterone and prolactin began to decrease 4 and 6 h respectively, after the pre‐ovulatory peak of LH.
5. Ovulation and oviposition occurred 6 to 8 h and 36.10+ 0.57 h (SEM) ( n= 11) respectively after the pre‐ovulatory peak of LH.
6. In birds kept on 14 h light/d, pre‐ovulatory peaks of LH were initiated only during a 10 to 11‐h period starting within 2 h after the onset of darkness.
7. A comparison between these data and those from the fowl suggest that the egg is retained in the turkey's oviduct for about 3 to 4 h longer than in the fowl. 相似文献
2. Although the protein content of all tissues varied with age, the only statistically significant effect was on the level of protein in the gizzard.
3. The moisture content of the gizzard was significantly affected by age and blood by age and sex.
4. The fat content of the gizzard was significantly affected by age, as was the effect of sex on the fat content of blood.
5. The ash contents of heart and gizzard were significantly affected by age.
6. The phosphorus contents of the gizzard, heart and liver were significantly affected by age, as were the chloride content of heart and the calcium content of the liver. Blood potassium was significantly affected by sex. Fluctuations in the contents of other elements are discussed.
7. The variability in organ composition is discussed with respect to the degree of trimming and preparation of the samples. 相似文献
2. Food passage in the crop was significantly reduced by intraperitoneal (IP) injection of LPS while it did not affect the number of defecations, suggesting that LPS may affect food passage only in the upper digestive tract.
3. Similar to LPS, prostaglandin E2 (PGE2), one of the mediators of LPS, also reduced crop-emptying rate in chickens while it had no effect on the number of defecations.
4. Pretreatment with indomethacin, which is an inhibitor of cyclooxygenase (COX), a prostaglandin synthase, had no effect on LPS-induced inhibition of crop emptying.
5. IP injection of LPS did not affect the mRNA expression of COX2 in the upper digestive tract of chickens.
6. It is therefore likely that LPS and PGE2 reduced food passage rate in the crop by a prostaglandin-independent pathway in chickens. 相似文献
2. Protein and energy metabolism were studied in males from these selected lines fed on a diet of intermediate protein content.
3. All selected birds retained more nitrogen than those studied 10 years previously.
4. There was no difference in nitrogen retention between groups, although relative growth rate of group B birds was higher.
5. Heat productions relative to gross energy intake were 0.38 (group B) and 045 (group A). Energy retentions relative to gross energy intake were 0.39 (group B) and 0.35 (group A); the difference being primarily due to higher fat retention in group B.
6. Using a common maintenance requirement for metabolisable energy, group B utilised metabolisable energy for growth (0.78) better than did group A (0.71).
7. At 53 d of age plasma glucose (10%) and insulin (50%) were higher in group B than in group A. 相似文献