Effect of supplementing a high-protein ram press sunflower cake concentrate on smallholder milk production in Zimbabwe

Ram press sunflower cake protein concentrate was formulated locally and its effect on the performance of dairy cows was compared with that of a commercial dairy concentrate. The effects of concentrate on roughage dry matter intake, milk yield and milk composition, live weight and calving intervals were similar for all concentrates, which indicated that the home grown dairy concentrates were as effective as the dairy commercial concentrate. The lack of significant differences in the concentrations of rumen NH₃-N, pH, VFAs and total AA in all the cows between concentrate types supported this. The results indicated that in the low-resource and low-input production systems, crossbreds were as productive as Jersey and Red Dane cows. The exotic cows were more susceptible to tick-borne diseases and mastitis, and showed higher incidences of calving problems and retained placenta compared with crossbred cows. The economics of feeding ram press sunflower concentrate were compared with those of a commercial dairy concentrate on the performance of crossbred, Red Dane and Jersey cows. The gross margin was higher when feeding sunflower cake than when feeding commercial dairy concentrate (p < 0.05).     

Effects of urea treatment of straw and dietary level of vegetable oil on lactating dairy cows

Four crossbreds (75% Holstein Friesian) lactating dairy cows were used to evaluate the effects of sunflower oil (SFO) levels and roughage source on feed intake, nutrient digestibility, ruminal fermentation, milk yield, and milk composition. Four milking cows with average liveweight of 410 ± 25 kg and 18 ± 11 days in milk were randomly assigned according to a 4 × 4 Latin square design, in a 2 × 2 factorial arrangement, with SFO levels (3% or 6%) in the concentrate and the roughage source [rice straw (RS) or urea-treated RS (UTRS)] being the main factors. Four dietary treatments as (1) 3% SFO + RS, (2) 6% SFO + RS, (3) 3% SFO + UTRS, and (4) 6% SFO + UTRS were offered ad libitum total mixed ration, with a concentrate/roughage ratio of 60:40. The results were found that UTRS as a roughage source significantly increased feed intake, digestibility, concentration of acetic acid in rumen fluid, rumen ammonia–nitrogen, blood–urea nitrogen, milk urea–nitrogen, and milk yield (3.5% fat-corrected milk) compared with cows fed on untreated RS. Supplementation of SFO at 3% in the concentrate-supplemented group having increased dry matter intake, milk fat percentage, and milk yield (3.5% fat-corrected milk) compared with 6% SFO supplementation. However, there were no interaction effects between level of SFO in the concentrate and roughage source in any of the factors studied.     

Effect of silage from ryegrass intercropped with winter or common vetch for grazing dairy cows in small-scale dairy systems in Mexico

The objective was to determine the effect of including silages of annual ryegrass (Lolium multiflorum) intercropped with winter vetch (Vicia villosa) (ARG-VV) or with common vetch (Vicia sativa) (ARG-VS) compared with maize silage (MS) on milk yield and milk composition of dairy cows grazing cultivated perennial ryegrass–white clover pastures with supplemented concentrate during the dry season. Six Holstein dairy cows with a mean yield of 19.0 kg/cow/day at the beginning of the experiment were randomly assigned to a 3 × 3 repeated Latin square. Treatments were: 8 h/day intensive grazing, 3.6 kg of dry matter (DM) per cow per day of concentrate plus MS, and ARG-VV or ARG-VS ad libitum at a stocking rate of 3.0 cows/ha for three experimental periods of 3 weeks each. Milk yield (MY) and milk composition, live weight and body condition score as well as silage and concentrate intakes were recorded during the third week of each experimental period, and pasture intake was estimated indirectly from utilised metabolisable energy. Economic analysis was obtained by preparing partial budgets. There were no statistical differences (P > 0.10) in MY, milk fat or protein content nor for live weight, but there was significant difference (P < 0.10) in body condition score. There were non-statistical differences in silage DM intake (P < 0.11); however, significant differences (P < 0.10) were obtained for estimated grazed herbage intake whilst no differences for total DM intake. Slightly higher economic returns (10%) were obtained with ARG-VS over MS, and this was 7% higher than ARG-VV. It is concluded that ARG-VS could be an option for complementing grazing for small-scale dairy production systems in the dry season as it is comparable to MS in animal performance and slightly better in economic terms.     

Feeding <Emphasis Type="Italic">Moringa oleifera</Emphasis> fresh or ensiled to dairy cows—effects on milk yield and milk flavor

Moringa oleifera, either fresh or ensiled, was compared with Elephant grass as a main feedstuff for dairy cows. To test the effects feed had on milk yield, milk composition, ration digestibility, and the organoleptic characteristics of milk, six lactating dairy cows were used in a Changeover 3 × 3 Latin Square experiment, replicated twice. With equal intake of metabolizable energy the intake of protein and fiber differed (p < 0.001) between all diets where fresh Moringa had the highest and the Elephant grass diet had the lowest intake. Compared with the control diet, ensiled Moringa had higher digestibility (P < 0.05) of both protein and fiber. With the exception of DM digestibility, no digestibility differences were found between fresh Moringa and Moringa silage treatments. Milk yield did not differ between any of the treatments and averaged 13.7 kg cow day⁻¹. Milk composition was similar among all treatments. Milk from the fresh Moringa treatment, however, had a grassy flavor and aroma, significantly different from the other two treatments, even though it was normal in color and appearance. No organoleptic differences were found between milk from the control treatment and the Moringa silage treatment. The conclusion is that Moringa silage can be fed to dairy cows in large quantities to produce the same quantity and quality of milk as traditional diets.     

苜蓿鲜草和青饲玉米对奶牛生产性能和乳品质的影响

探讨日粮中添加苜蓿鲜草和青饲玉米对奶牛生产性能及其乳成分的影响,本试验选用荷斯坦泌乳奶牛20头,随机分为2组,对照组饲喂秸秆和精料补充料;试验组饲喂苜蓿鲜草、青饲玉米和精料补充料日粮。试验结果表明,日粮中添加苜蓿鲜草和青饲玉米,可极显著提高奶牛的生产性能和乳脂率(P<0.01),显著提高干物质采食量和乳蛋白含量(P<0.05)。     

Energy intake and requirement of dairy cows during the whole year

It is argued that in view of the many new methods in dairy farming an economic model of the dairy farm should be available to improve advisory work. Some quantitative data concerning the dairy cow needed for such a model are given. They deal with a net energy feeding value of grass, hay, silage, artificially dried grass and concentrates estimated with a feed evaluation system based on results obtained with lactating cows. Also data on the requirements for net energy and for long forage dry matter are given, again obtained with such cows. Finally information is given on voluntary intake of forage dry matter at pasture, from fresh grass fed indoors, from various preserved forages including forage pellets, as influenced by milk yield and condition of the cow and by kind of forage and level of concentrates fed. Special attention has been paid to the precision of the information. These data are used to predict both the maximal and minimal amounts of long forage which can be fed in the stall period without loss of production, and the additional quantities of concentrates needed. For the grazing cow the problem of the adjustment of voluntary intake of grass to milk yield and amount of concentrates fed is studied. It is concluded that the information is sufficient to develop an economic model of a dairy farm. For its development grassland research should supply data on pasture yield in terms of digestible organic matter when the forage is fed to sheep at the maintenance level, and the economists should add data on costs of food and labour.     

Effects of inclusion of whole-crop pea silages differing in their tannin content on the performance of dairy cows fed high or low protein concentrates

The effect of including high or low tannin forage peas as an alternative to soyabean meal on performance and N efficiency was investigated using 18 dairy cows in two Latin square design studies. Two varieties of forage pea; Racer (coloured flowers; high tannin) and Croma (white flowers; low tannin), were ensiled 12 weeks post sowing. The in situ degradability characteristics of the pea silages were determined using 4 cannulated sheep. The low tannin forage had a higher soluble N (a) fraction and a greater extent of degradation (a + b), resulting in a greater amount of N being predicted to be released in the rumen than the high tannin forage. In experiment 1, cows were fed one of three treatments in each of 3 periods of 28 d duration in a Latin square design; grass silage/whole-crop wheat (0.50:0.50, DM basis) and 8 kg/d of a high protein concentrate (GWH); grass silage/whole-crop wheat/high tannin forage pea silage (0.25:0.25:0.50 respectively, DM basis) and 8 kg/d of a high protein concentrate (HTH) or 8 kg/d of a low protein supplement (HTL). The inclusion of forage peas increased (P < 0.05) DM intake by 1.2 kg DM/d, but there was no effect of dietary treatment on milk yield (average of 23.0 kg/d), composition or live weight change. The efficiency of use of dietary N (N output in milk/N intake) was highest (P < 0.001) in cows when fed GWH (0.289) and lowest in HTH (0.234). In experiment 2, cows were fed one of three treatments in each of 3 periods of 28 d duration in a Latin square design; grass silage/whole-crop wheat (0.50:0.50, DM basis) and 8 kg/d of a high protein concentrate (GWH); grass silage/whole-crop wheat/low tannin forage pea silage (0.25:0.25:0.5 respectively, DM basis) and 8 kg/d of a high protein concentrate (LTH) or 8 kg/d of a low protein concentrate (LTL). Compared with GWH, the inclusion of forage peas increased (P < 0.05) DM intake by 2.0 kg/d, but there was no effect of dietary treatment on milk yield (average of 24.5 kg/d). Milk fat content was highest (P < 0.05) in cows when fed LTL (45.7 g/kg) and lowest in LTH (41.9 g/kg), but there was no effect (P > 0.05) of treatment on daily fat yield or any other milk component. The efficiency of dietary N use was highest (P < 0.001) in cows when fed GWH (0.300) and lowest in LTH (0.234). It is concluded that either high or low tannin forage pea silages can replace approximately 1.1 kg/d of soyabean meal in the diet of mid-late lactation dairy cows without affecting animal performance, but N efficiency for milk production is decreased.     

Association of Mycobacterium avium subspecies paratuberculosis infection with milk production and calving interval in Iranian Holsteins

There are inconsistent results for the association of Mycobacterium avium subspecies paratuberculosis (MAP) infection with production and reproduction in dairy cows. Determination of these associations in each region is essential to encourage participation of dairy cattle producers in disease control programs. This study was conducted in Shiraz, southern Iran, to quantify the association of subclinical MAP infection with 305-day milk production and calving interval in Iranian Holsteins. A total of 21 dairy herds were selected for the study and in each herd, quarter milk samples were collected from ten to 12 dairy cows for PCR analysis. Data about parity, calving interval, length of lactation period, total milk production and 305-day milk production were also provided for each animal. Overall, 252 individual milk samples were collected. Herd- and individual-level prevalence of MAP infection were 23.8% (95% CI, 6.2–41.4%) and 3.2% (95% CI, 1.3–5.1%), respectively based on IS900 nested PCR. The results for 305-day milk production revealed a 248 kg reduction in positive cows compared with negative ones (P = 0.009). When cows from positive herds were compared with cows from negative herds, a 335-kg reduction in 305-day milk production (P = 0.005) and a 30-day increase in calving interval (P = 0.057) were observed in the former group. These findings support the previous results that paratuberculosis infection is negatively associated with the performance of the animals.     

Milk yield and quality in Guernsey cows fed cottonseed cake-based diets partially substituted with baobab (<Emphasis Type="BoldItalic">Adansonia digitata</Emphasis> L.) seed cake

The objective of this study was to determine the effects of partially substituting cottonseed cake with graded levels of baobab (Adansonia digitata L.) seed cake (BSC) on milk yield and quality in Guernsey cows. Sixteen cows in mid-lactation and in their third parity were allocated to diets containing 0% (control), 5%, 10%, and 15% BSC in a completely randomized design. Each cow was given a daily feed ration of 6 kg and a basal diet of soya bean stover ad libitum. There were no differences in daily feed intake (P > 0.05), but basal intake differed among all treatment groups with cows on the control diet having the highest intake (30 ± 0.34 kg/day). Mean daily milk yield differed (P < 0.05) among all treatment groups. However, the control had higher milk yield of 12.1 ± 0.73 kg/day, and the 15% BSC had the least yield of 7.46 ± 0.73 kg/day. Cows on the control diet had higher milk butterfat content (6.12%; P < 0.05) than those on the BSC-based diets. Protein content differed (P < 0.05) across all treatment groups with cows on 15% BSC producing the highest protein content (3.43%) while the control had the least (2.6%). The concentration of milk total solids for cows fed on 15% BSC was higher (P < 0.05) than that from cows on other diets. Lactose content was not affected by the diets (P > 0.05). These results indicate that BSC can substitute soya bean cake in dairy diets, but milk production and butterfat content are compromised.     

A comparison of dairy cattle systems in an irrigated perimeter and in a suburban region: case study from Morocco

Multivariate analyses were used to compare dairy production practices and their consequences on milk yield and profitability in cattle farms from two representative regions of Morocco. A regular follow-up of 118 farms (48 in the Rabat-Salé suburban belt and 70 in the Gharb irrigated perimeter) was undertaken to obtain accurate data. Results show significant differences between the two regions. Intensive milk production was more frequent in the suburban zone (more concentrates and better annual milk yield per cow). When conducting a “within-region” principal components analysis, farms’ discrimination appeared to take into account all management variables (feeding, cattle sales, profitability), with no reference to farms’ structural parameters (arable land and number of cattle). A typology of farms was then established using cluster analysis, with 4 distinct groups, namely: a) concentrates wasters, b) farms with a relatively important milk yield per cow, c) deficit dairy farms and d) beef oriented farms. The last group included almost exclusively farms from the irrigated perimeter (5 out of 7). These results indicate that dairy production promotion in Morocco requires more than just the intensification of forage production, but should focus also on improving management practices. The extension of complete and balanced dairy rations is urgently needed to enhance milk yield and profitability.     

Effect of using Acacia mearnsii tannin extract as a feed additive on nutritional variables and productive performance in dairy cows grazing a temperate pasture

This study was carried out to evaluate the impact of including Acacia mearnsii tannin extract (TA) as a feed additive on nutrition and productive performance of dairy cows grazing a high‐quality temperate pasture and receiving supplementation with a concentrate feedstuff. Fourteen multiparous Holstein cows were assigned to either of the following treatments: concentrate without or with 20 g TA/kg dry matter (DM). Concentrate intake accounted for 32% of the total DM intake. Tannin addition increased the herbage DM intake by 22% (p < .05). There was no effect of TA inclusion on milk yield, milk composition, milk nitrogen (N) excretion, milk and plasma urea‐N concentration, urinary excretion of total N, urea‐N, and purine derivatives. However, TA inclusion increased the N intake and retention, total N excretion in manure, fecal N to urine N ratio, and decreased the dietary N efficiency for milk production and the percentage of ingested N excreted in urine (p < .05). In conclusion, supplementing dairy cows grazing a high‐quality temperate pasture with a concentrate containing 20 g TA/kg DM showed the potential of decreasing the proportion of ingested N excreted in urine without affecting the productive performance.     

Comparative evaluation of raw and roasted soybean in lactating crossbred cows

An experiment was conducted to evaluate the effect of roasting whole soybean in the local level on their chemical composition, nitrogen fractions, in situ degradability, and feeding performance in crossbred (Karan Swiss and Karan Fries) dairy cows. Twelve lactating crossbred cattle (369.08 ± 17.53 kg body weight) were randomly divided into two groups on the basis of milk yield (10.18 kg/day) and day of calving (89 days) in a randomized block design. Cows were fed green maize fodder and concentrate mixtures with 1 kg raw soybean as fed basis (7.6% of total diet) in the control group, where as roasted soybean replaced raw soybean in the treatment group to make both diets isonitrogenous and isocaloric. The roughage to concentrate ratio was around 55:45 in both diets. The cows were housed in individual byres and fed experimental diet for 90 days. The total dry matter intake per animal in both groups was similar. The digestibility of dry matter, organic matter, crude protein, ether extract, and neutral detergent fiber was comparable in both groups. The average milk production and fat-corrected milk yield per day was similar during the trial. Milk fat, milk protein, and solids not fat content remained unaffected and similar in both groups. All the animals maintained their body weight in both groups. Thus, rumen bypassing of protein fractions at the present method of bypassing did not have the desired level of beneficial effect in medium producing crossbred cows.     

Protein supplementation to early lactation dairy cows grazing tropical grass: Performance and ruminal metabolism

This experiment was designed to evaluate the effects of different concentrate crude protein (CP) concentration on performance, metabolism and efficiency of N utilization (ENU) on early-lactation dairy cows grazing intensively managed tropical grass. Thirty cows were used in a ten replicated 3 × 3 Latin square design. The treatments consisted of three levels of concentrate CP: 7.9%, 15.4%, and 20.5% offered at a rate of 1 kg (as-fed basis)/3 kg of milk. The cows fed low and medium CP had negative balance of rumen degradable protein and metabolizable protein. Increasing CP tended to linearly increase DMI, 3.5% FCM and milk casein, and linearly increased the yields of milk fat and protein. Increasing CP linearly increased the intake of N, the concentration of rumen NH₃–N, and the losses of N in milk, urine, and feces. Increasing dietary CP linearly increased the molar proportion of butyrate but had no effect on the other rumen VFAs and no effect on microbial yield. In conclusion, feeding a concentrate with 20.5% of CP to early-lactation dairy cows grazing tropical grasses, leading to a 17.8% CP diet, tended to increase DMI, increased the yield of 3.5% FCM and the milk N excretion, and decreased ENU by 32%.     

The effect of energy supplementation on nitrogen utilization in lactating dairy cows fed grass silage diets

An experiment was conducted to examine the effect that various isoenergetic diets, containing different quantities of soluble carbohydrate and fiber and different types of starch, have on nitrogen (N) balances. Six lactating dairy cows in early to midlactation consuming grass silage diets with not less than 600 g/kg total DMI as forage were used in the experiment. Four concentrates were prepared that had higher amounts of either fiber, soluble sugars, corn (low degradable starch source), or barley (high degradable starch source). Overall N utilization by the cows was poor, rarely exceeding 0.30 g milk N/g of dietary N intake. Fecal N outputs accounted for more than half of total N excreted in all treatments except for diets supplemented with high degradable starch, in which urinary N excretion was significantly higher compared with the other treatments. Milk yield was unaffected by concentrate type, averaging 19.9 kg/d, but milk protein content decreased from 32.9 for starch-based diets to 30.9 and 30.0 g/kg for the soluble sugar- and fiber-based diets, respectively. The efficiency of N utilization improved in the low degradable starch treatment, which had lower N excretion (65%) and higher protein concentration in milk. Furthermore, feeding cows corn-based concentrates reduced urinary N excretion by almost 30% compared with barley-based concentrates; therefore, feeding corn-based diets is recommended for the reduction of nitrogen pollution in lactating dairy cows.     

Feed intake and production parameters of lactating crossbred cows fed maize-based diets of stover,silage or quality protein silage

Thirty-six Boran × Friesian dairy cows (392 ± 12 kg; mean ± SD) in early parity were used in a randomised complete block design. Cows were blocked by parity into three blocks of 12 animals and offered normal maize (NM) stover (T1), NM silage (T2) or quality protein maize (QPM) silage (T3) basal diets supplemented with a similar concentrate mix. Feed intake, body weight and condition changes and milk yield and composition were assessed. The daily intake of DM, OM, NDF and ADF for cows fed the NM stover-based diet was higher (P < 0.05) than for the cows fed the NM silage and QPM silage-based diets. However, the daily intake of DOM (9.3 kg) and ME (140.8 MJ) for cows on QPM silage-based diet was higher (P < 0.05) than for cows on NM stover-based diet (8.4 kg and 124.2 MJ) and NM silage-based diet (7.9 kg and 119.1 MJ). Body weight of cows was affected (P < 0.05) by the diet, but diet had no effect (P > 0.05) on body condition score, milk yield and milk composition. The digestible organic matter in the NM stover-based diet (724 g/kg DM) was lower (P < 0.05) than that in the NM (770 g/kg DM) and QPM silage-based diet (762 g/kg DM). It was concluded that the performances of the cows on the NM silage and QPM silage diets were similar and were not superior to that of the NM stover-based diet.     

Oats (Avena strigosa) as winter forage for dairy cows in Vietnam: an on-farm study

In North Vietnam, during winter, alternative forage resources are needed to balance the feed ration of dairy cows. The objective of this study was to investigate the effect of oat forage as a component of traditional winter roughage diets on feed intake, milk production and feeding cost in dairy cows. The study was conducted on-farm using 24 mid-lactation Holstein–Friesian cows. The feeding experiment consisted of two successive periods and two dietary treatments per period. Traditional basal diets included fresh tropical grasses, maize silage and hay. The oat forage had no effect on the dry matter intake of the basal diet, but the total crude protein intake was higher in cows fed with oat diets than in those fed with control diets. The yield of butterfat-corrected milk (FCM) was not significantly different between diets during period 1, but there was a trend (P?=?0.078) of higher FCM yields in cows fed with the oat diet compared to those with control diet during period 2 (17.3 vs. 16.3 kg/day). The decline rate in milk yield was significantly higher (P?<?0.001) in cows fed with control diets than in those fed with oat diets in both experimental periods. The total feeding cost of cows fed with oat diets was on average 12 % lower than those fed with control diets (P?<?0.01). So, the oat forage is an important winter resource for cows in North Vietnam allowing higher milk yield whilst reducing feeding cost, compared to traditional roughage diets.     

The prediction of the intake potential of grass silage in the supplemented diets of lactating dairy cows

This study was undertaken to develop models which could be used in conjunction with the near infrared reflectance spectroscopy (NIRS) analysis of grass silage to accurately predict the intake potential of grass silage when offered to lactating dairy cows as part of a mixed diet. Empirical models were developed with data collected from two large-scale studies carried out at the Institute. The models comprised of (1) a linear equation for converting the NIRS-based predicted intake of a given silage for beef cattle to dairy cows and (2) a model which corrected the intake potential of the grass silage for supplementary concentrates. Furthermore, a milk yield adjustment factor of 0.14 kg DM/kg milk was utilised to standardise milk yields. Both linear and exponential models were developed to describe the decrease in silage intake as concentrate intake increased, with y-axis intercepts corresponding to unsupplemented silage intakes (NIRS-based predictions for beef cattle adjusted for dairy cows) and common x-axis intercept of 168.0 (SE=20.50) and 203.8 (SE=5.64) g/kg W^0.75, respectively, corresponding to concentrate intake when offered as a sole feed. A common r parameter (model curvature) of 1.0047 (SE=0.00397) was assumed for the exponential model. When the models were validated against the data from an independent study, the predictions from the two models were not significantly different, giving R² values of 0.70. The intercept and slope from the linear model were 5.39 and 1.01, respectively, and the intercept and slope from the exponential model were 6.10 and 0.98, respectively. Both intercepts and slopes were not significantly different from 0 and 1, respectively. Ninety-three percent of predictions were within 10% of observed intakes in the validation data.     

Impact of diet composition and temperature–humidity index on water and dry matter intake of high‐yielding dairy cows

The temperature–humidity index (THI) is widely used to characterize heat stress in dairy cattle. Diet composition is known to induce variation in metabolic‐associated heat production. However, the relationships between THI and diet are poorly characterized with regard to performance and intake behaviour. Therefore, the objectives were to evaluate the impact of THI on water intake (WI), dry matter intake (DMI) and the frequency of drinking and feeding bouts in lactating dairy cows offered four dietary treatments: each contained 20% grass silage and additionally (i) 20% maize silage, 60% concentrate (M‐HC); (ii) 60% maize silage, 20% concentrate (M‐LC); (iii) 20% pressed beet pulp silage, 60% concentrate (BPS‐HC); or (iv) 60% pressed beet pulp silage, 20% concentrate (BPS‐LC) (DM basis). Individual WI and DMI were recorded from April to July 2013. Furthermore, dietary effects on milk production and reticular pH were estimated. Milk yield was lowest for M‐LC, while energy‐corrected milk was similar for all diets. Milk fat percentage was higher and milk protein amount lower for cows offered both LC diets. Reticular pH below 6.3, 6.0 and 5.8 lasted longest for BPS‐LC. WI was higher for HC diets. However, the frequency of drinking bouts was not influenced by the ration. Lower DMI occurred for BPS‐LC compared to M‐LC. Frequency of feeding bouts was significantly higher for LC diets. THI was significantly related to WI, DMI as well as drinking and feeding bouts. Per increasing THI, WI increased slightly more for LC diets and DMI decreased more for HC diets. Frequency of drinking bouts increased slightly higher for BPS rations per rising THI, while the decrease in feeding bouts was highest for M‐HC. In conclusion, TMR composition and moderate heat stress impacted WI and DMI of dairy cows, while both dietary energy density and ruminal filling might intensify the THI impact.     

Effect of cereal-based concentrates on productivity of Holstein–Friesian cows grazing short-rotation ryegrass (Lolium multiflorum) or kikuyu (Pennesitum clandestinum) pastures

In this study, the effect of increasing the proportion of concentrate in the diet, on efficiency of feed utilisation, was determined when Holstein–Friesian cows grazed short-rotation ryegrass (Lolium multiflorum) or kikuyu (Pennisetum clandestinum) pastures. The concentrates were energy-dense dairy pellets fed twice-a-day at milking and the roughage component was lucerne hay and the pasture.When cows grazed ryegrass, there was no effect on animal performance as the proportion of concentrate in the diet increased from 0.23 to 0.35 (4.75 to 7.50 kg concentrate/cow/day). The substitution rate of concentrates for pasture for the first 1.57 kg concentrate/cow/day fed was 0.58 but rose to 1.18 for the next 1.28 kg concentrate/cow/day.When cows grazed kikuyu, there was also no effect of increasing the proportion of concentrate in the diet on total dry matter intake (DMI) or milk production. However, there was a substantial increase in the in vivo digestibility of whole diet, pasture and acid detergent fibre (ADF) was observed when the proportion of concentrate in the diet increased from 0.08 to 0.25. However, there was a marked decline in pasture digestibility (72% to 64%), and more so in ADF digestibility (61.3% to 48.4%), as the proportion of concentrate in the diet increased further to 0.29 (5.52 kg/cow/day). The intake of kikuyu, when determined by difference between pre- and post-grazing pasture mass, was substantially underestimated compared to the use of the n-alkane technique, and this discrepancy increased as the pasture on offer increased.On both pasture types, the neutral detergent fibre intake, as a % of bodyweight varying from 1.6% to 2.2% for kikuyu and 1.5% to 1.6% for ryegrass was far above the values claimed of 1.2% to restrict intake.The results of this study highlight the limits to the amount of concentrate that can be fed on a typical Australian dairy farm where concentrates can only be fed twice-a-day at milking. The results also provide a more appropriate benchmark for fibre limitation in the ration when cows graze pasture, particularly poorer quality tropical grasses, and this value is well above that found in more intense feeding situations.