Nitrogen best management practice for citrus trees: I. Fruit yield,quality, and leaf nutritional status

Elevated levels of nitrate-nitrogen (NO₃-N) in the surficial aquifer above the drinking water quality standard, i.e. maximum contaminant limit (MCL; 10 mg L⁻¹), have been reported in some part of central Florida citrus production regions. Soils in this region are very sandy (sand content >95%), hence are vulnerable to leaching of soluble nutrients and chemicals below the rooting depth of the trees. The objective of this research was to develop N and irrigation best management practices for citrus in sandy soils to maintain optimal crop yield and quality, and to minimize potential leaching of nitrate below the root zone. Six years of field experiment was conducted in a high productive (mean fruit yield > 80 Mg ha⁻¹yr⁻¹) >20-year-old ‘Hamlin’ orange trees [Citrus sinensis (L.) Osbeck] on ‘Cleopatra mandarin’ (Citrus reticulata Blanco) rootstock grown on a well drained Tavares fine sand (hyperthermic, uncoated, Typic Quartzipsamments) in Highland county, FL. Nitrogen rates ranged from 112 to 280 kg ha⁻¹ yr⁻¹ applied as fertigation (FRT), water soluble granular (WSG), 50:50 mix of FRT and WSG, and controlled-release fertilizer (CRF). Tensiometers were used to monitor the soil water content as a basis to schedule optimal irrigation. Fruit yield response over the entire range of N rates was greater for the FRT and WSG sources as compared to that for the WSG + FRT or CRF sources. Using the regression analysis of the fruit yield in relation to N rate, the optimum N rate appeared to be at 260 kg ha⁻¹ yr⁻¹. Based on fruit production response in this study, the N requirement for production of 1 Mg of fruit varied from 2.2 to 2.6 kg across four N sources. This study demonstrated an increased N uptake efficiency, as a result of best management of N and irrigation applications. The optimal N and K concentration in the 4–6-month-old spring flush leaves were 26–30, and 15–18 g kg⁻¹, respectively. However, fruit yield response showed no significant relationship with concentrations of P in the 4–6-month-old spring flush leaves over a range of 0.8–2.4 g kg⁻¹. The results of fate and transport of N in soil and in soil solution with application of different rates and sources of N, and components of citrus tree N budget, are reported in a companion paper.     

Effect of synthetic auxins on fruit development of ‘Bing’ cherry (Prunus avium L.)

The main cherry cultivar grown in the warm climate of Israel, ‘Bing’, produces relatively small fruit. Over three consecutive years (2003–2005), application of 50 mg l⁻¹ 2,4-dichlorophenoxypropionic acid [2,4-DP; as its butoxyethyl ester (Power™)], 10 mg l⁻¹ 3,5,6-trichloro-2-pyridyloxyacetic acid [3,5,6-TPA; as the free acid (Maxim^®)], or 25 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) plus 30 mg l⁻¹ naphthaleneacetic acid (NAA; 0.3% Amigo™), at the beginning of pit-hardening when fruitlet diameter was ca. 13 mm caused appreciable and significant increases in fruit size and total yield, except when the crop load was heavy. Anatomical studies revealed that the main effect of these synthetic auxins was via direct stimulation of fruit cell enlargement. The above auxins had no negative effect on fruit quality, either at harvest or after 1 month of storage at 0 °C, or on return yield in the following year.     

Improvement of soil properties using compost for optimum parsley production in sandy soils

In the warm and wet north Florida climate, growing Italian parsley (Petroselinum crispum [Mill.]) is gaining popularity with small producers as a short duration crop on sandy soil. Application of compost to agricultural land can benefit the low fertile sandy soils in Florida and subsequent crop production, while providing an outlet for recycling municipal solid wastes (MSW) and biosolids. A field study was laid out in a randomized complete block design with four replications to evaluate the effects of compost (75% MSW:25% biosolids) application in comparison to fertilizer, fertilizer + compost (50:50), and control treatments on: (a) parsley fresh weight (FW), (b) soil and plant tissue nutrient concentrations, and (c) soil bulk density and moisture retention in winter and spring seasons. Soil amended with fertilizer or compost + fertilizer doubled parsley FW from 15.02 Mg ha⁻¹ in the non-amended control plot to 30.75 and 32.67 Mg ha⁻¹ in soils that received fertilizer + compost or fertilizer alone, respectively. Significantly higher total soil carbon (C) levels of 2.16% and 1.95% and nitrogen (N) levels of 0.19% and 0.16% were recorded in compost and fertilizer + compost treatments, respectively. Addition of compost reduced soil bulk density significantly to 1.03 Mg m⁻³ and increased soil moisture retention in simulated drier conditions at 1500 kPa to 0.12 m³ m⁻³ in plots that received only compost at the end of winter growing season. Overall, addition of compost resulted in improvement of both physical and chemical properties as well as increased parsley yields.     

CPPU application on size and quality of hardy kiwifruit

For the purpose of determining the appropriate conditions of application to increase the size of a hardy kiwifruit, Actinidia arguta ‘Mitsuko’, N₁-(2-chloro-4-pyridyl)-N₃-phenylurea (CPPU) was applied at three different growth stages of the crop: at petal fall, 10 and 25 days after petal fall (DAPF), and three different concentrations: 1, 5 and 10 mg l⁻¹. A significant increase in fruit size was obtained by treatment at the concentrations of 5–10 mg l⁻¹ and at 10 DAPF. The fruit weight doubled. Although a significant reduction in the concentrations of total soluble solids (TSS), titratable acids (TA) and ascorbic acid (AsA) in the CPPU-treated fruits was recorded, the TSS/TA ratio and AsA content per fruit increased by the treatment. CPPU application at petal fall induced abnormally protruding fruit tip.     

Pollen load affects fruit set,size, and shape in cherimoya

Cherimoya (Annona cherimola Mill.) is a subtropical fruit crop that exhibits protogyny dichogamy and for which the original pollinators are not present in the main producing countries. Under such circumstances the achievement of profitable yields depends on hand pollination. The present work explores the effects of the amount of pollen applied on pollen–pistil interaction and on fruit set, size, shape and growth. Pollen dilution with Lycopodium spores reduced fruit set and size and favoured the appearance of misshapen fruits, whereas the application of double amount of pollen did not improve fruit size and increased fruit set only the first year. Pollen load did not affect fruit growth model, which always followed a double sigmoid pattern. Nevertheless, the slopes of the curves during the two rapid growth phases were higher when a larger amount of pollen grains was applied. Seed index (seed number per 100 g of fresh fruit weight) rose slightly as pollination level increased.     

Root zone soil nitrogen management to maintain high tomato yields and minimum nitrogen losses to the environment

Greenhouse field experiments on tomato were carried out at Shouguang, Shandong province, over four double cropping seasons between 2004 and 2008 in order to understand the effects of manipulating root zone N management (RN) on fruit yields, N savings and N losses under conventional furrow irrigation. About 72% of the chemical N fertilizer used in conventional treatment (CN) inputs could be saved using the RN treatment without loss of yield. The cumulative fruit yields were significantly higher in the RN treatment than in the CN treatment. Average seasonal N from irrigation water (118 kg N ha⁻¹), about 59% of shoot N uptake, was the main nitrogen source in treatments with organic manure application (MN) and without organic manure or nitrogen fertilizer (NN). N losses in the RN treatment were lowered by 54% compared with the CN treatment. Lower N losses were found in the MN and NN treatments due to excessive inputs of organic manure and fruit yields were consequently substantially affected in the NN treatment. The critical threshold of N_min supply level in the root zone (0–30 cm) should be around 150 kg N ha⁻¹ for sustainable production. April to May in the winter–spring season and September to October in the autumn–winter season are the critical periods for root zone N manipulation during crop growth. However, control of organic manure inputs is another key factor to further reduce surplus N in the future.     

Influence of fruit load on dry matter and N-distribution in sweet pepper plants

The influence of fruit load on the leaf characteristics and on the distribution of dry matter (DM) and nitrogen (N) in sweet pepper (Capsicum annuum L., cv. Cornado) plants was investigated under Mediterranean glasshouse conditions during a winter–spring production cycle (from December till June). DM weight of all organs (roots, stems + petioles, leaves and fruits) and N content were determined throughout the growing cycle over a 3-week interval. The results showed that the cyclic fruit load pattern (production flushes) was associated with strong variations of N-leaf content on an area basis and specific leaf weight, SLW. On a whole plant scale, the value of SLW dramatically decreased (≈30%) with increasing fruit load; the reverse held true as long as the fruit dry weight remained low (<30 g pl⁻¹). The decline in SLW was coincident with a shift in distribution of DM and N in the plant, both preferentially diverted to the fruits. The time evolution of DM and N fractions in organs exhibited periodic fluctuations, with a duration close to the length of a fruit growth cycle (about 70 days, from anthesis to harvest). Linear relationships were found between the fraction of dry mass and N in fruits, and those of the other organs. The slope of these relationships was considered as an indicator of the response and sensitivity of the organ growth to an increase in fruit load. The root dry mass fraction was the most affected by increases in fruit load (slope of −0.75), while the N fraction in roots and in leaves was affected to a similar extent (slope of −0.50 and of −0.40, respectively). We conclude that, in the sweet pepper, the cyclic pattern of fruit load induces opposite cyclic patterns of dry matter and N content in the other aerial organs as well as in the roots, thereby reflecting close shoot–root coordination in the allocation of carbon and nitrogen resources among the organs. Finally, we discuss the possible implications that could derive from these findings for modelling dry matter partitioning in plants subjected to continuous fruit harvesting.     

Potential of ethephon,NAA, NAD and urea for thinning pistachio fruitlets

Alternate bearing, the occurrence of high yield ‘on’ year followed by low yield ‘off’ year, is striking in pistachio (Pistacia vera L.). Floral buds of pistachio are formed a year before bloom, but abscise during the years with heavy crop (‘on’ year). Abscission of floral buds is due to competition between growing seeds on 1-year-old shoots and developing buds on current season growth. We studied the effects of chemical fruit thinning on alternate bearing and nut characteristics in a commercial orchard of ‘Owhadi’ pistachio cultivar during 2003–2004 in Rafsanjan, Iran. In both years, ethephon (100 and 200 mg l⁻¹), naphthaleneacetic acid (NAA) (125 and 250 mg l⁻¹) and urea (2.5 and 5%) were applied to the branch units of each individual ‘on’-year trees. The results showed that ethephon at both concentrations significantly increased fruit thinning and floral bud retention for the subsequent year. Other treatments also increased fruit thinning and floral bud retention but were inferior to ethephon.     

Effect of organic and inorganic fertilizers applied during successive crop seasons on growth and nitrate accumulation in lettuce

A romaine-type lettuce (Lactuca sativa L.) cv. Corsica was cultivated during three successive crop seasons (late-spring, late-autumn and late-winter) in the same soil of an experimental greenhouse in S.W. Peloponnese, Greece. Seven long-term fertilization treatments were tested for their effect on plant growth and nitrate concentration in the external lettuce leaves. Treatments included: three different doses of organic fertilization (composted sheep manure) applied at the start of each crop season, three different doses of inorganic N fertilization applied via fertigation during each crop season, and a control treatment in which no fertilizer was applied. A drip irrigation system was used to water all plants. The highest nitrate levels were observed in the medium and maximum inorganic fertilization treatments (572–664 mg kg⁻¹) in all crop seasons. They were significantly higher compared to the respective organic fertilization treatments (253–435 mg kg⁻¹) and all other fertilization treatments (148–435 mg kg⁻¹). Crop season affected lettuce growth more than nitrate accumulation in the lettuce leaves: lettuce biomass production was the smallest and most uniform in the late-autumn season and did not respond to the fertilization treatments tested (ranging from 409 to 439 g plant⁻¹), while in the late-spring season biomass production was the highest and most variable (561–841 g plant⁻¹), it correlated with nitrate concentration in the leaves and in the medium and maximum inorganic fertilizer doses it significantly exceeded production from all other fertilization treatments (827–841 g plant⁻¹). Following the three crop seasons the residual availability of N, P and K was clearly enhanced in the soil receiving the organic compared to the inorganic fertilization. Nitrate concentration in lettuce leaves was far below the upper limits set by the European Commission in all fertilization treatments throughout the three crop seasons, a result attributed mainly to the sufficient level of light intensity and duration throughout the year in Southern Greece.     

Determination of the uptake and translocation of nitrogen applied at different growth stages of a melon crop (Cucumis melo L.) using N isotope

In order to establish a rational nitrogen (N) fertilisation and reduce groundwater contamination, a clearer understanding of the N distribution through the growing season and its dynamics inside the plant is crucial. In two successive years, a melon crop (Cucumis melo L. cv. Sancho) was grown under field conditions to determine the uptake of N fertiliser, applied by means of fertigation at different stages of plant growth, and to follow the translocation of N in the plant using ¹⁵N-labelled N. In 2006, two experiments were carried out. In the first experiment, labelled ¹⁵N fertiliser was supplied at the female-bloom stage and in the second, at the end of fruit ripening. Labelled ¹⁵N fertiliser was made from ¹⁵NH₄¹⁵NO₃ (10 at.% ¹⁵N) and 9.6 kg N ha⁻¹ were applied in each experiment over 6 days (1.6 kg N ha⁻¹ d⁻¹). In 2007, the ¹⁵N treatment consisted of applying 20.4 kg N ha⁻¹ as ¹⁵NH₄¹⁵NO₃ (10 at.% ¹⁵N) in the middle of fruit growth, over 6 days (3.4 kg N ha⁻¹ d⁻¹). In addition, 93 and 95 kg N ha⁻¹ were supplied daily by fertigation as ammonium nitrate in 2006 and 2007, respectively. The results obtained in 2006 suggest that the uptake of N derived from labelled fertiliser by the above-ground parts of the plants was not affected by the time of fertiliser application. At the female-flowering and fruit-ripening stages, the N content derived from ¹⁵N-labelled fertiliser was close to 0.435 g m⁻² (about 45% of the N applied), while in the middle of fruit growth it was 1.45 g m⁻² (71% of the N applied). The N application time affected the amount of N derived from labelled fertiliser that was translocated to the fruits. When the N was supplied later, the N translocation was lower, ranging between 54% at female flowering and 32% at the end of fruit ripening. Approximately 85% of the N translocated came from the leaf when the N was applied at female flowering or in the middle of fruit growth. This value decreased to 72% when the ¹⁵N application was at the end of fruit ripening. The ammonium nitrate became available to the plant between 2 and 2.5 weeks after its application. Although the leaf N uptake varied during the crop cycle, the N absorption rate in the whole plant was linear, suggesting that the melon crop could be fertilised with constant daily N amounts until 2–3 weeks before the last harvest.     

Nitrogen best management practice for citrus trees: II. Nitrogen fate,transport, and components of N budget

Soils in central Florida citrus production region are very sandy, hence are vulnerable to leaching of soluble nutrients and chemicals. The objective of this study was to develop nitrogen (N) and irrigation best management practices for citrus in sandy soils to maintain optimal crop yield and quality, and to minimize N leaching below the rootzone. A replicated plot experiment was conducted in a highly productive 20+ year-old ‘Hamlin’ orange [Citrus sinensis (L.) Osbeck] trees on ‘Cleopatra mandarin’ [(Citrus reticulata Blanco)] rootstock grove located on a well drained Tavares fine sand (hyperthermic, uncoated, Typic Quartzipsamments) in Highland County, FL. Nitrogen rates (112–280 kg ha⁻¹ year⁻¹) were applied as fertigation (FRT), water soluble granular (WSG), a combination of 50% FRT and 50% WSG, and controlled release fertilizer (CRF). Tensiometers were used to monitor the soil moisture content at various depths in the soil profile as basis to optimize irrigation scheduling. Fruit yield and quality and nutritional status of the trees were reported in a companion paper. Soil solution was sampled at 60, 120, and 240 cm depths under the tree canopy using suction lysimeters. Concentrations of NO₃-N in the soil solution at 240 cm deep, which is an indicator of NO₃-N leaching below the tree rootzone, generally remained below the maximum contaminant limit (MCL) for drinking water quality (10 mg L⁻¹) in most samples across all N sources and rates, but for few exceptions. Total N in the fruit was strongly correlated with fruit load, thus, at a given N rate N removal by the fruit was lower during years of low fruit yield as compared to that during the years of high fruit yield. Furthermore, there was a strong linear relation between N and K in the fruit. This supports the need to maintain 1:1 ratio between the rates of N and K applications. In a high fruit production condition, the N in the fruit accounted for about 45% of the total N input on an annual basis. Fifteen percent of the total N input at 280 kg N ha⁻¹ year⁻¹ was not accounted for in the citrus N budget, which could be due to leaching loss. This estimate of potential leaching was very close to that predicted by LEACHM simulation model. The improved N and irrigation management practices developed in this study contributed to an improved N uptake efficiency and a reduction in N losses.     

Comparative effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on growth and cell wall peroxidase activity in tomato fruits

The effects of regulated deficit irrigation (RDI) and partial root-zone drying (PRD) on tomato fruit growth and cell wall peroxidase activity in tomato exocarp were investigated in growth chamber conditions. The RDI treatment was 50% of water given to fully irrigated (FI) plants and the PRD treatment was 50% of water of FI plants applied to one half of the root system while the other half dried down, with irrigation shifted when soil water content of the dry side decreased 15–20%. RDI significantly reduced fruit diameter, though PRD reduced fresh weight while having no significant effect on fruit diameter. The activity of peroxidase was significantly higher in RDI and PRD treated plants compared to those of FI. Differences between RDI and PRD were expressed on temporal basis. In the fruits of RDI treated plants peroxidase activity began to increase in the phase when fruit growth started to decline with the peak of enzyme activity of 6.1 HRPEU g⁻¹ FW reached in the phase of mature green fruits when fruit growth rate was minimal. Increase of peroxidase activity in PRD fruits coincided with the ripening phase and the peak of enzyme activity (5.3 HRPEU g⁻¹ FW) was measured at the end of fruit ripening. These data potentially identified contrasting and different roles of tomato exocarp cell wall peroxidase in RDI and PRD treated plants. In RDI treated plants peroxidase may have a role in restricting fruit growth rate, although the increase in enzyme activity during ripening of PRD treated fruit pointed out that peroxidase may also control fruit maturation by inducing more rapid process.     

Fruit growth,cuticle deposition,water uptake,and fruit cracking in jostaberry,gooseberry, and black currant

Fruit cracking after rain limits the production of a number of crops, including some Ribes species. To gain a better understanding of the factors involved in cracking, fruit growth, deposition of the cuticular membrane (CM), water uptake and fruit cracking were studied in black currant (Ribes nigrum L. cv. Zema), gooseberry (Ribes uva-crispa L. cv. Rote Triumph), and jostaberry (Ribes nidigrolaria B. cv. Jostine). Fruit surface area and fresh mass increased continuously throughout development, whereas deposition of the CM was biphasic. CM mass per fruit increased rapidly up to 42, 41, and 49 days after full bloom (DAFB) in black currant, gooseberry, and jostaberry, respectively. Thereafter, CM mass per fruit remained constant in gooseberry and jostaberry or increased at a lower rate in black currant. The cessation of or reduced rate of CM deposition resulted in a decrease in CM mass per unit area in all berries. Elastic strain of the CM at maturity averaged 23.8% and 19.5% in gooseberry and jostaberry, respectively, and only 8.2% in black currant. Microcracks in the CM were observed first in gooseberry and jostaberry 64 DAFB, whereas there were no microcracks in black currant. Water uptake into mature detached berries was linear over 2 h of incubation. Rates of uptake were highest in gooseberry followed by black currant and jostaberry. Relative uptake was similar via the cut end of the pedicel (32.1%), the apex of the fruit (34.7%) and the fruit surface (33.2%). Rates of water uptake through the fruit surface were positively related to surface area. Average fruit water potential for black currant, gooseberry, and jostaberry was −2.14 ± 0.17, −1.24 ± 0.03, and −1.89 ± 0.20 MPa, while the permeability for osmotic water uptake was 7.7 ± 0.4 × 10⁻⁸, 5.2 ± 0.1 × 10⁻⁸, and 3.3 ± 0.3 × 10⁻⁸ m s⁻¹. Incubating whole fruit in deionized water for 72 h resulted in more cracked jostaberries (94%) than black currants (74%) or gooseberries (50%). A comparison of our findings in Ribes berries with published data for the sweet cherry drupe revealed that the berries fitted the relationships established in sweet cherry among fruit growth, cuticle deposition, strain of the cuticle, microcracking, permeability for osmotic water uptake, frequency of stomata and cracking. The Ribes berries were less susceptible to cracking than sweet cherry.     

Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars

Pomegranate is one of the native fruits of Iran which contains high genetic resources, but there are insufficient information regarding properties of the fruit. The objective of the present study was to investigate the physcio-chemical characteristics and antioxidant activity of twenty pomegranate cultivars grown in Iran. This study showed that there were significant differences among the cultivars in all measured factors except the length/diameter ratio of fruit. The fruit weight, skin percentage, aril percentage and juice percentage were within the range of 196.89–315.28 g, 32.28–59.82%, 37.59–65% and 26.95–46.55%, respectively. The total soluble solids content varied from 11.37 (°Brix) to 15.07 (°Brix), pH values from 3.16 to 4.09, titratable acidity content from 0.33 g 100 g⁻¹ to 2.44 g 100 g⁻¹ and total sugars content from 13.23 g 100 g⁻¹ to 21.72 g 100 g⁻¹. The results also showed that the values of ascorbic acid ranged from 9.91 mg 100 g⁻¹ to 20.92 mg 100 g⁻¹. The total anthocyanins content was observed in pomegranate cultivars between 5.56  mg 100 g⁻¹ and 30.11 mg 100 g⁻¹. The level of total phenolics was varied from 295.79 mg 100 g⁻¹ to 985.37 mg 100 g⁻¹. The antioxidant activity of pomegranate cultivars was found between 15.59 and 40.72%. These data demonstrated that the cultivar was the main parameter which influences the physico-chemical properties and antioxidant activity in pomegranates.     

Nursery inoculation of tomato with arbuscular mycorrhizal fungi and subsequent performance under irrigation with saline water

Protected horticultural crops as well as those planted in open fields particularly in the Mediterranean region have to cope with increasing salinization of irrigation water. High salinity of the supply water has detrimental effects on soil fertility and plant nutrition and reduces crop growth and yield. This study was conducted to determine if pre-inoculation of transplants with arbuscular mycorrhizal (AM) fungi alleviates salt effects on growth and yield of tomato (Lycopersicon esculentum Mill. Cv. Marriha) when irrigated with saline water. Tomato seeds were sown in polystyrene trays with 20 cm³ cells and treated with AM fungi (AM) or without (nonAM) Glomus mosseae. Once the seedlings were reached appropriate size, they were transplanted into nonsterile soil in concrete blocks (1.6 m × 3 m × 0.75 m) under greenhouse conditions. The soil electrical conductivity (EC_e) was 1.4 dS m⁻¹. Plants were irrigated with nonsaline water (EC_w = 0.5 dS m⁻¹) or saline water (EC_w = 2.4 dS m⁻¹) until harvest. These treatments resulted with soil EC at harvest 1.7 and 4.4 dS m⁻¹ for nonsaline and saline water treatments, respectively. Root colonization with AM fungi at flowering was lower under saline than nonsaline conditions. Pre-inoculated tomato plants with AM fungi irrigated with both saline and nonsaline water had greater shoot and root dry matter (DM) yield and fruit fresh yield than nonAM plants. The enhancement in fruit fresh yield due to AM fungi inoculation was 29% under nonsaline and 60% under saline water conditions. Shoot contents of P, K, Zn, Cu, and Fe were higher in AM compared with nonAM plants grown under nonsaline and saline water conditions. Shoot Na concentrations were lower in AM than nonAM plants grown under saline water conditions. Results indicate that pre-inoculation of tomato transplants with AM fungi improved yield and can help alleviate deleterious effects of salt stress on crop yield.     

Characteristics and oleocellosis sensitivity of citrus fruits

The effects of variety, growth phase, and water loss on development of oleocellosis, and relationships between chromatism and Vis/NIR spectra were studied in ‘EarlyGold’ sweet orange (Citrus sinensis Osbeck), ‘Fukumoto’ navel (Citrus sinensis Osbeck), and ‘Cara Cara’ navel (Citrus sinensis Osbeck) oranges. The varieties showed significant differences in the rate (RO) and degree (DO) of oleocellosis development. The sensitivity of varieties (from most to least sensitive) was ‘EarlyGold’ > ‘Fukumoto’ > ‘Cara Cara.’ Growth phase and water loss had a significant influence on fruit sensitivity to oleocellosis. The order of sensitivity to oleocellosis was dependent on harvest time (i.e., at normal period > at delayed period > at uncolored period), and RO and DO decreased significantly with water loss. The RO and DO models for fruit water loss were established as y = 0.75 − 3.94x − 271.33x² (R² = 0.77) and y = 1.70 − 7.29x − 1025.83x² (R² = 0.583). The sensitivity of ‘EarlyGold’ to oleocellosis was significantly correlated with dL and dC of fruit chromatism. At the same time, there were significant differences at 480–575 nm, 650–720 nm, and 925–965 nm between varieties with low and high sensitivity to oleocellosis, and ‘EarlyGolds’ with a low RO and DO had a higher reflectance than those with a high RO and DO.     

Priming abiotic factors for optimal hybrid Cymbidium (Orchidaceae) PLB and callus induction,plantlet formation,and their subsequent cytogenetic stability analysis

Abiotic factors affect the induction of PLBs and callus in hybrid Cymbidium Twilight Moon ‘Day Light’. The initiation and proliferation of new PLBs and callus could be achieved on NAA and kinetin, supplemented at 0.1 mg l⁻¹ each, respectively, both within 45–60 days. Bacto agar was found to be the most suitable solidifying agent for PLB induction, although a higher shoot fresh weight was obtained on Gelrite; a pH 5.3 was optimal while pH 4.5 caused 100% explant necrosis; coconut water, when supplied at 10–20% (v/v) resulted in a significant increase in the number of PLBs formed per PLB segment (23.1 versus 14.6 in controls) while a massive (almost four-fold) increase in fresh top weight occurred when PLB explants were placed in liquid culture, as a result of hyperhydricity; Fe-EDTA (1 mg l⁻¹) and activated charcoal (1 g l⁻¹) stimulated total fresh weight and PLB formation in the presence of PGRs; PLB formation decreased but total fresh shoot weight increased with the addition of niacin or myo-inositol, both vitamins. Dark-grown PLB-induced plants were etiolated and had longer internodes and higher fresh weight than light-grown control plants at 45 μmol m⁻² s⁻¹; at 15 μmol m⁻² s⁻¹ shoots were slightly etiolated, fragile, and PLB formation was scarce. RAPD and mtDNA analysis of all resultant PLBs, callus or plants showed them to be genetically identical, with comparable chlorophyll contents. Despite the detection of cytological variation between different plant parts, little variation resulted from abiotic factor treatment.     

Onion yield and quality response to two irrigation scheduling strategies

Irrigation technologies that conserve water are necessary to assure the economic and environmental sustainability of commercial agriculture. This study was conducted in the Rio Grande Valley in Texas to evaluate yield and quality of subsurface drip irrigated onions (Allium cepa L.) using different scheduling strategies and water stress levels. One strategy consisted of initiating irrigation when the reading of a granular matrix sensors (Watermark^{® 1} soil moisture sensor, Irrometer, Co., Riverside, CA) installed at 0.2 m depth reached −20 kPa (optimum), −30 kPa and −50 kPa. The second strategy was to replace 100%, 75%, and 50% of crop evapotranspiration (ETc) weekly. Higher total yields, and jumbo onion size yields were obtained when the soil moisture was kept above −30 kPa. Yields were not affected when water applications were reduced from 100% to 75% ETc and from −20 to −30 kPa. The ETc strategies of 100%, and 75% ETc resulted in similar water usage to the soil moisture monitoring strategies of initiating irrigation at −20 and −30 kPa. Total yields dropped significantly when soil water stress increased below −50 kPa. For the ET based strategy yields also dropped with the 50% ETc treatment. Onion bulb pungency and brix were unaffected by water level.     

Triacontanol stimulates nitrogen-fixation,enzyme activities,photosynthesis, crop productivity and quality of hyacinth bean (Lablab purpureus L.)

Hyacinth bean (Lablab purpureus L.) serves as a good source of vegetable proteins in human diet, and its seeds and pods contain as much as 20–28% protein. The bean contains tyrosinase enzyme, which has potential use for the treatment of hypertension. However, plant biological yield appears to be comparatively low in Aligarh soil in this region of India (Western Uttar Pradesh). A hypothesis was designed to address whether foliar application of triacontanol (TRIA) could enhance the crop productivity as well as crop quality. TRIA is known to be a potent plant growth promoting substance for many agricultural and horticultural crops. The hyacinth bean plants grown in soil containing pots, were sprayed with five concentrations of TRIA (10⁻⁰ (Control), 10⁻⁸, 10⁻⁷, 10⁻⁶ and 10⁻⁵ M) at 15-day intervals. The plant fresh and dry weights, leaf-area, number and dry weight of nodules per plant, total chlorophyll and carotenoid content, nitrate reductase activity, carbonic anhydrase activity, nodule-nitrogen content, leghemoglobin content and leaf N, P, K and Ca contents were analyzed at 60, 90 and 120 days after sowing (DAS). Net photosynthetic rate, transpiration rate and stomatal conductance were measured only at 90 DAS. The protein content plus carbohydrate and tyrosinase activity were analyzed in the seeds. Foliar spray of 10⁻⁶ M TRIA significantly stimulated most of the studied attributes. At the highest concentration (10⁻⁵ M TRIA), values of all attributes were significantly decreased at all three stages. For example, 10⁻⁶ M TRIA increased seed-yield and -protein content by 56.3 and 14.5%, respectively when compared to unsprayed plants. TRIA also stimulated the activity of tyrosinase when compared to the control plants.     

Effects of greenhouse cooling method on growth,fruit yield and quality of tomato (Solanum lycopersicum L.) in a tropical climate

A tomato (Solanum lycopersicum L.) crop was grown in four greenhouses during the dry season 2005/06 in Central Thailand. Sidewalls and roof vents of two greenhouses were covered with nets and these greenhouses were mechanically ventilated when air temperature exceeded 30 °C (NET). The other two greenhouses were covered with polyethylene film and equipped with a fan and pad cooling system (EVAP). Overall mean air temperature was significantly reduced by 2.6 and 3.2 °C (day) and 1.2 and 2.3 °C (night) in EVAP as compared to NET and outside air, respectively. Temperature maxima in EVAP averaged about 4 °C lower than in NET and outside. The relative humidity was around 20 and 30% (day) and 10 and 15% (night) higher in EVAP than in NET or outside, respectively. Vapour pressure deficit averaged 0.25 kPa in EVAP, 1.03 kPa in NET and 1.48 kPa outside. The crop water-consumption was significantly lower in EVAP (1.2) than in NET (1.8 L plant⁻¹ day⁻¹), which is ascribed to reduced transpiration in EVAP. Total fruit yield was similar in NET (6.4 kg plant⁻¹) and EVAP (6.3 kg plant⁻¹). The quantity of undersized (mostly parthenocarpic) and blossom-end rot (BER)-affected fruits was reduced in EVAP. However, the proportion of marketable yield was significantly higher in NET (4.5 kg plant⁻¹) than in EVAP (3.8 kg plant⁻¹), owing largely to an increased incidence of fruit cracking (FC) in EVAP. Higher FC but lower BER incidence coincided with higher fresh weight and Ca concentration in the fruits in EVAP. It is concluded that in regions with high atmospheric relative humidity evaporative cooling without technical modifications allowing dehumidification will not improve protected tomato production.