共查询到20条相似文献,搜索用时 258 毫秒
1.
Mutations in a protein kinase C homolog confer phorbol ester resistance on Caenorhabditis elegans 总被引:4,自引:0,他引:4
The tpa-1 gene mediates the action of tumor-promoting phorbol esters in the nematode Caenorhabditis elegans. A genomic fragment that constitutes a portion of the tpa-1 gene was cloned by Tc1 transposon tagging and was used as a probe to screen a nematode complementary DNA library. One of the isolated complementary DNA clones had a nucleotide sequence that predicts a polypeptide of 526 amino acids. The predicted amino acid sequence revealed that the predicted tpa-1 protein sequence is highly similar to protein kinase C molecules from various animals, including man. 相似文献
2.
Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer's disease 总被引:109,自引:0,他引:109
D Goldgaber M I Lerman O W McBride U Saffiotti D C Gajdusek 《Science (New York, N.Y.)》1987,235(4791):877-880
Four clones were isolated from an adult human brain complementary DNA library with an oligonucleotide probe corresponding to the first 20 amino acids of the beta peptide of brain amyloid from Alzheimer's disease. The open reading frame of the sequenced clone coded for 97 amino acids, including the known amino acid sequence of this polypeptide. The 3.5-kilobase messenger RNA was detected in mammalian brains and human thymus. The gene is highly conserved in evolution and has been mapped to human chromosome 21. 相似文献
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Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization 总被引:78,自引:0,他引:78
M Jaye R Howk W Burgess G A Ricca I M Chiu M W Ravera S J O'Brien W S Modi T Maciag W N Drohan 《Science (New York, N.Y.)》1986,233(4763):541-545
Several of the endothelial cell polypeptide mitogens that have been described probably play a role in blood vessel homeostasis. Two overlapping complementary DNA clones encoding human endothelial cell growth factor (ECGF) were isolated from a human brain stem complementary DNA library. Southern blot analysis suggested that there is a single copy of the ECGF gene and that it maps to human chromosome 5 at bands 5q31.3 to 33.2 A 4.8-kilobase messenger RNA was present in human brain stem messenger RNA. The complete amino acid sequence of human ECGF was deduced from the nucleic acid sequence of these clones; it encompasses all the well-characterized acidic endothelial cell polypeptide mitogens described by several laboratories. The ECGF-encoding open reading frame is flanked by translation stop codons and provides no signal peptide or internal hydrophobic domain for the secretion of ECGF. This property is shared by human interleukin-1, which is approximately 30 percent homologous to ECGF. 相似文献
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Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B 总被引:117,自引:0,他引:117
S M Ruben P J Dillon R Schreck T Henkel C H Chen M Maher P A Baeuerle C A Rosen 《Science (New York, N.Y.)》1991,251(5000):1490-1493
7.
Interferon-beta-related DNA is dispersed in the human genome 总被引:6,自引:0,他引:6
A D Sagar P B Sehgal L T May M Inouye D L Slate L Shulman F H Ruddle 《Science (New York, N.Y.)》1984,223(4642):1312-1315
Interferon-beta 1 (IFN-beta 1) complementary DNA was used as a hybridization probe to isolate human genomic DNA clones lambda B3 and lambda B4 from a human genomic DNA library. Blot-hybridization procedures and partial nucleotide sequencing revealed that lambda B3 is related to IFN-beta 1 (and more distantly to IFN-alpha 1). Analyses of DNA obtained from a panel of human-rodent somatic cell hybrids that were probed with DNA derived from lambda B3 showed that lambda B3 is on human chromosome 2. Similar experiments indicated that lambda B4 is not on human chromosomes 2, 5, or 9. The finding that DNA related to the IFN-beta 1 gene (and IFN-alpha 1 gene) is dispersed in the human genome raises new questions about the origins of the interferon genes. 相似文献
8.
Salivary proline-rich protein genes on chromosome 8 of mouse 总被引:1,自引:0,他引:1
Endonuclease restriction (Hind III) fragments of DNA from Chinese hamster X mouse somatic cell hybrids hybridized with proline-rich protein complementary DNA clones only when the DNA was isolated from cells containing mouse chromosome 8, or a fragment of chromosome 8. The evidence suggests that proline-rich protein genes are located at the proximal portion of chromosome 8 toward the centromere. 相似文献
9.
Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins 总被引:185,自引:0,他引:185
G G Wong J S Witek P A Temple K M Wilkens A C Leary D P Luxenberg S S Jones E L Brown R M Kay E C Orr 《Science (New York, N.Y.)》1985,228(4701):810-815
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA. 相似文献
10.
提取衣藻总 DNA,以此为模板并参照肌动蛋白基因编码区端的序列合成寡聚核苷酸引物,进行聚合酶链式反应,扩增到一个1.2 kb 的 DNA 片段.将此片段进行克隆并经分子探针杂交,证明此片段为衣藻肌动蛋白基因.经限制性核酸内切酶处理,构建了衣藻肌动蛋白基因的物理图谱.根据物理图谱进行亚克隆以后,测得了编码区5′端的618个核苷酸的顺序.衣藻肌动蛋白基因的编码序列与高等植物之间的同源性大于90%,高于与高等动物、原生动物及真菌的同源性.但在基因的结构上,衣藻肌动蛋白基因又明显地不同于高等植物,在已经测定的基因片段上,没有发现内含子的存在.经限制性内切酶片段多态性分析,衣藻中含有一个肌动蛋白基因拷贝. 相似文献
11.
DNA sequence of two closely linked human leukocyte interferon genes 总被引:16,自引:0,他引:16
R M Lawn J Adelman T J Dull M Gross D Goeddel A Ullrich 《Science (New York, N.Y.)》1981,212(4499):1159-1162
12.
Identification of a novel thyroid hormone receptor expressed in the mammalian central nervous system 总被引:41,自引:0,他引:41
A complementary DNA clone derived from rat brain messenger RNA has been isolated on the basis of homology to the human thyroid hormone receptor gene. Expression of this complementary DNA produces a high-affinity binding protein for thyroid hormones. Sequence analysis and the mapping of this gene to a distinct human genetic locus indicate the existence of multiple human thyroid hormone receptors. Messenger RNA from this gene is expressed in a tissue-specific fashion with highest levels in the central nervous system. 相似文献
13.
M Trahey G Wong R Halenbeck B Rubinfeld G A Martin M Ladner C M Long W J Crosier K Watt K Koths 《Science (New York, N.Y.)》1988,242(4886):1697-1700
The ras p21 GTPase-activating protein (GAP) was purified from human placental tissue. Internal amino acid sequence was obtained from this 120,000-dalton protein and, by means of this sequence, two types of complementary DNA clones were isolated and characterized. One type encoded GAP with a predicted molecular mass of 116,000 daltons and 96% identity with bovine GAP. The messenger RNA of this GAP was detected in human lung, brain, liver, leukocytes, and placenta. The second type appeared to be generated by a differential splicing mechanism and encoded a novel form of GAP with a predicted molecular mass of 100,400 daltons. This protein lacks the hydrophobic amino terminus characteristic of the larger species, but retains GAP activity. The messenger RNA of this type was abundantly expressed in placenta and in several human cell lines, but not in adult tissues. 相似文献
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Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase 总被引:27,自引:0,他引:27
A complementary DNA clone encoding the alpha subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the alpha subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in Gs alpha activity, are devoid of this messenger RNA. 相似文献
15.
Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells 总被引:46,自引:0,他引:46
G T Merlino Y H Xu S Ishii A J Clark K Semba K Toyoshima T Yamamoto I Pastan 《Science (New York, N.Y.)》1984,224(4647):417-419
The sequence of the human epidermal growth factor (EGF) receptor shows great homology with the avian erythroblastosis virus v-erb B oncogene, raising the possibility that the receptor gene is identical to the c-erb B protooncogene. Human A431 epidermoid carcinoma cells, which have an unusually high number of EGF receptors, were examined to determine whether elevated EGF receptor levels correlate with gene amplification. Southern blots of genomic DNA's from A431 and other human cell lines were probed with either a v-erb B gene fragment or a human EGF receptor complementary DNA clone (pE7), previously isolated from an A431 complementary DNA library. When either probe was used to analyze Eco RI- or Hind III-generated DNA fragments, EGF receptor DNA sequences were amplified about 30-fold in A431. Differences in the banding pattern of A431 DNA fragments relative to normal fibroblast DNA indicate the occurrence of a rearrangement in the region of the receptor gene. Furthermore, A431 cells contain a characteristic, prominent 2.9-kilobase RNA. These results are consistent with the hypothesis that, in A431 cells, gene amplification, possibly associated with a translocation event, may result in the overproduction of EGF receptor protein or the appearance of the transformed phenotype (or both). 相似文献
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Purification and complementary DNA cloning of a receptor for basic fibroblast growth factor 总被引:78,自引:0,他引:78
P L Lee D E Johnson L S Cousens V A Fried L T Williams 《Science (New York, N.Y.)》1989,245(4913):57-60
Basic fibroblast growth factor (bFGF) participates in many processes including early developmental events, angiogenesis, wound healing, and maintenance of neuronal cell viability. A 130-kilodalton protein was isolated on the basis of its ability to specifically bind to bFGF. A complementary DNA clone was isolated with an oligonucleotide probe corresponding to determined amino acid sequences of tryptic peptide fragments of the purified protein. The putative bFGF receptor encoded by this complementary DNA is a transmembrane protein that contains three extracellular immunoglobulin-like domains, an unusual acidic region, and an intracellular tyrosine kinase domain. These domains are arranged in a pattern that is different from that of any growth factor receptor described. 相似文献
18.
Molecular cloning of the chicken progesterone receptor 总被引:16,自引:0,他引:16
O M Conneely W P Sullivan D O Toft M Birnbaumer R G Cook B L Maxwell T Zarucki-Schulz G L Greene W T Schrader B W O'Malley 《Science (New York, N.Y.)》1986,233(4765):767-770
To define the functional domains of the progesterone receptor required for gene regulation, complementary DNA (cDNA) clones encoding the chicken progesterone receptor have been isolated from a chicken oviduct lambda gt11 cDNA expression library. Positive clones expressed antigenic determinants that cross-reacted with six monospecific antibodies derived from two independent sources. A 36-amino acid peptide sequence obtained by microsequencing of purified progesterone receptor was encoded by nucleotide sequences in the longest cDNA clone. Analysis of the amino acid sequence of the progesterone receptor deduced from the cDNA clones revealed a cysteine-rich region that was homologous to a region found in the estrogen and glucocorticoid receptors and to the avian erythroblastosis virus gag-erb-A fusion protein. Northern blot analysis with chicken progesterone receptor cDNA's indicated the existence of at least three messenger RNA species. These messages were found only in oviduct and could be induced by estrogens. 相似文献
19.
Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif 总被引:67,自引:0,他引:67
L M Staudt R G Clerc H Singh J H LeBowitz P A Sharp D Baltimore 《Science (New York, N.Y.)》1988,241(4865):577-580
20.
Targets for dioxin: genes for plasminogen activator inhibitor-2 and interleukin-1 beta 总被引:18,自引:0,他引:18
Dioxin (2,3,7,8-tetrachlorodibenzo-p-dioxin, TCDD), a widespread environmental contaminant, may elicit its effects by altering gene expression in susceptible cells. Five TCDD-responsive complementary DNA clones were isolated from a human keratinocyte cell line. One of these clones encodes plasminogen activator inhibitor-2, a factor that influences growth and differentiation by regulating proteolysis of the extracellular matrix. Another encodes the cytokine interleukin-1 beta. Thus, TCDD alters the expression of growth regulatory genes and has effects similar to those of other tumor-promoting agents that affect both inflammation and differentiation. 相似文献