Effect of plantain on nitrous oxide emissions and soil nitrification rate in pasture soil under a simulated urine patch in Canterbury,New Zealand

Purpose

The aim of this research was to quantify the effect of plantain (Plantago lanceolata L.) on soil nitrification rate, functional gene abundance of soil ammonia oxidisers, and the concomitant effect on nitrous oxide emissions from urine patches in a shallow, free-draining soil in Canterbury during late autumn/winter season.

Materials and methods

Urine was collected from dairy cows grazing either ryegrass/white clover (RGWC), 30% plantain (P30) mixed in with RGWC or 100% plantain (P100) pasture, and applied at two rates (700 or 450 kg N ha^?1) to intact soil blocks growing either RGWC, P30 or P100 pasture.

Results and discussion

Results showed that increased plantain content reduced N-concentration in urine from 7.2 in RGWC urine to 4.5 and 3.7 g N L^?1 in P30 and P100 urine, respectively. Total N₂O emissions and emission factors (EF₃) from urine-treated pastures were low, <?2 kg N ha^?1 and <?0.22%, respectively. Urine application at the lower urine N-loading rate of 450 kg N ha^?1 (i.e. representative of that in a P30 urine patch) resulted in 30% lower N₂O emissions (P?<?0.01) and 35% lower soil nitrate concentrations (P?<?0.001) compared to those at the higher urine loading rate of 700 kg N ha^?1 (i.e. representative of that in a RGWC urine patch). Increasing plantain content in the pasture sward from 0 to 30% and 100% with urine N applied at the same loading rate did not reduce N₂O emissions or nitrification compared to the standard ryegrass-white clover pasture. Cow urine derived from the different pasture diets had no effect on N₂O emissions, N transformation or ammonia-oxidiser abundance in soil compared to the RGWC urine applied at the same rate.

Conclusions

The main effect of plantain in this study appears to be related to the reduction in urine N-loading rate, rather than factors related to urine properties or plantain-soil interactions.

     

Effects of forage type and gibberellic acid on nitrate leaching losses

Nitrogen (N) leaching from soil into water is a significant concern for intensively grazed forage‐based systems because it can cause a decline in water quality and is a risk to human health. Urine patches from grazing animals are the main source of this N. The objective of this study was to quantify the effect that forage type and gibberellic acid (GA) application had on N leaching and herbage N uptake from urine patches on perennial ryegrass–white clover (RGWC), Italian ryegrass and lucerne. A lysimeter study was conducted over 17 months to measure herbage growth, N uptake and N loss to water beneath each of the three forage types with the following treatments: control, urine (700 kg N/ha) and urine with GA (8 g GA active ingredient/ha). Compared with RGWC (205 kg N/ha), N leaching losses were 35.3% lower from Italian ryegrass (133 kg N/ha) and 98.5% higher from lucerne (407 kg N/ha). These differences in leaching loss are likely to be due to winter plant growth and N uptake. During the winter months, Italian ryegrass had higher N uptake, whereas lucerne had lower N uptake, compared with RGWC. The application of GA had no effect on N leaching losses, DM yield or N uptake of forage treated with 700 kg N/ha urine.     

The effect of grassland renovation on soil mineral nitrogen and on nitrate leaching during winter

Renovation of grassland may increase the mineralization of organic material and leads to a high amount of mineral N in soil which can be leached in the winter period. Soil mineral N (SMN) in autumn and calculated nitrate leaching during winter were measured after the renewal of 8 y–old cut grassland on a sandy soil in NW Germany in 1999 to 2002. Several factors, which may influence the intensity of N mineralization, were investigated in the 2 years following renewal: the season of renovation (spring or late summer/early autumn), the technique (rotary cultivator or direct drilling), and the amount of N fertilization (0 or 320 kg N ha^–1 y^–1 in the 7 years before the renovation). Calculated nitrate‐N leaching losses during winter were significantly higher following renewal in early autumn (36–64 kg N ha^–1) compared to renewal in spring (1–7 kg N ha^–1). This effect was only significant in the first, not in the second winter after renovation. The renovation technique had a significant effect on the nitrate‐N leaching losses only in the first year after the renovation. Direct drilling led to higher leaching losses (35 kg N ha^–1) than the use of a rotary cultivator (30 kg N ha^–1) in the same year. Calculated nitrate losses (on average over 60 kg N ha^–1) were highest after renewal of N‐fertilized grassland in late summer/early autumn. To minimize N leaching losses, it would be more effective to plan grassland renewal in spring rather than in late summer/autumn. Another, however, less effective option is to reduce N fertilization before a renovation in autumn.     

Nitrate leaching and residual soil nitrogen supply following outdoor pig farming

Abstract. Large nitrogen (N) inputs to outdoor pig farms in the UK can lead to high nitrate leaching losses and accumulation of surplus N in soil. We investigated the residual effects of three contrasting outdoor pig systems as compared to an arable control on nitrate leaching and soil N supply for subsequent spring cereal crops grown on a sandy loam soil during 1997/98 and 1998/99 harvest seasons. Previously, the pig systems had been stocked for 2 years from October 1995 and were designated current commercial practice (CCP, 25 sows ha^?1 on stubble), improved management practice (IMP, 18 sows ha^?1 on undersown stubble) and best management practice (BMP, 12 sows ha^?1 on established grass). Estimated soil N surpluses by the end of stocking in September 1997 were 576, 398, 265 and 27 kg ha^?1 N for the CCP, IMP, BMP and continuous arable control, respectively. Nitrate leaching losses in the first winter were 235, 198, 137 and 38 kg ha^?1 N from the former CCP, IMP and BMP systems and the arable control, respectively. These losses from the former pig systems were equivalent to 41–52% of the estimated soil N surpluses. Leaching losses were much smaller in the second winter at 21, 14, 23 and 19 kg ha^?1 N, respectively. Cultivation timing had no effect (P>0.05) on leaching losses in year 1, but cultivation in October compared with December increased nitrate leaching by a mean of 14 kg ha^?1 N across all treatments in year 2. Leaching losses over the two winters were correlated (P<0.001) with autumn soil mineral N (SMN) contents. In both seasons, spring SMN, grain yields and N offtakes at harvest were similar (P>0.05) for the three previous pig systems and the arable control, and cultivation timing had no effect (P>0.05) on grain yields and crop N offtake. This systems study has shown that nitrate leaching losses during the first winter after outdoor pig farming can be large, with no residual available N benefits to following cereal crops unless that first winter is much drier than average.     

Potassium leaching from cut grassland and from urine patches

In grassland farming, especially on coarse‐textured soils, K can be a critical element. On these soils, the actual K management as well as fertilizer history to a large extent determine the leaching of K. The effects of four fertilizer regimes on the nutrient balances and leaching of K from grassland grown on a sandy soil were investigated. The swards differed in the source and level of N input and K fertilizer: no fertilizer N + 166 kg K ha^?1 year^?1 (Control), 320 kg inorganic N ha^?1 + 300 kg K ha^?1 year^?1 (MIN 320), 320 kg N + 425 kg K ha^?1 year^?1 in form of cattle slurry (SLR 320) and a grass–clover sward + 166 kg K ha^?1 year^?1 (WCL 0) without any inorganic N input. In a second experimental phase, cores from these swards were used in a mini‐lysimeter study on the fate of K from urine patches. On cut grassland after 6 years K input minus removal in herbage resulted in average K surpluses per year of 47, 39, 56 and 159 kg K ha^?1 for the Control, MIN 320, WCL 0 and SLR 320, respectively. Related leaching losses per year averaged 7.5, 5, 15 and 25 kg K ha^?1. Losses of urinary‐K through leaching were 2.2–4.5 and 5.7–8.4% of the K supplied in summer and autumn applications, respectively. Plant and soil were the major sinks for K from fertilizer or urine. High levels of exchangeable K in the soil and/or large and late fertilizer or urine applications stimulated leaching of K.     

The effect of four different pasture species compositions on nitrate leaching losses under high N loading

Nitrate () leaching can cause elevated concentrations of ‐N in water, which can have adverse impacts on water quality and human health. In grazed pasture systems, most of the ‐N leaching occurs beneath animal urine‐N deposits. The objective of this study was to investigate the effect of four different pasture species compositions [perennial ryegrass/white clover (P. ryegrass WC), tall fescue/white clover (T. fescue WC), Italian ryegrass/white clover (It. ryegrass WC) and perennial ryegrass/Italian ryegrass/white clover/red clover/chicory/plantain (Diverse)] on ‐N leaching losses from animal urine patches, and to examine the relative importance of root system architecture and seasonal activity to reduce ‐N leaching losses. The results show that ‐N leaching losses were 24–54% lower beneath It. ryegrass WC than other pasture species. Total dry matter (DM) yield in the season following establishment was 11–58% greater in the It. ryegrass WC pasture, while average winter daily N uptake rate of It. ryegrass WC over the two seasons was on average 58% greater than P. ryegrass WC and T. fescue WC. In the second season, the P. ryegrass WC and T. fescue WC pastures had up to 140 and 82% more roots between 0 and 40 cm depth, respectively, than the other pasture species compositions. These results suggest that in grazed pasture systems, high plant winter activity (plant growth/root metabolic activity) is more important than specific root architecture (e.g. deep roots) to reduce ‐N leaching losses.     

Nitrogen losses from outdoor pig farming systems

Abstract. Nitrogen losses via nitrate leaching, ammonia volatilization and nitrous oxide emissions were measured from contrasting outdoor pig farming systems in a two year field study. Four 1‐ha paddocks representing three outdoor pig management systems and an arable control were established on a sandy loam soil in Berkshire, UK. The pig management systems represented: (i) current commercial practice (CCP) ‐ 25 dry sows ha^?1 on arable stubble; (ii) ‘improved’ management practice (IMP) ‐ 18 dry sows ha^?1 on stubble undersown with grass, and (iii) ‘best’ management practice (BMP) 12 dry sows ha^?1 on established grass. Nitrogen (N) inputs in the feed were measured and N offtakes in the pig meat estimated to calculate a nitrogen balance for each system. In the first winter, mean nitrate‐N concentrations in drainage water from the CCP, IMP, BMP and arable paddocks were 28, 25, 8 and 10 mg NO₃ l^?1, respectively. On the BMP system, leaching losses were limited by the grass cover, but this was destroyed by the pigs before the start of the second drainage season. In the second winter, mean concentrations increased to 111, 106 and 105 mg NO₃‐N l^?1 from the CCP, IMP and BMP systems, respectively, compared to only 32 mg NO₃‐N l^?1 on the arable paddock. Ammonia (NH₃) volatilization measurements indicated that losses from outdoor dry sows were in the region of 11 g NH₃‐N sow^?1 day^?1. Urine patches were identified as the major source of nitrous oxide (N₂O) emissions, with N₂O‐N losses estimated at less than 1% of the total N excreted. The nitrogen balance calculations indicated that N inputs to all the outdoor pig systems greatly exceeded N offtakes plus N losses, with estimated N surpluses on the CCP, IMP and BMP systems after 2 years of stocking at 576, 398 and 264 kg N ha^?1, respectively, compared with 27 kg N ha^?1 on the arable control. These large N surpluses are likely to exacerbate nitrate leaching losses in following seasons and make a contribution to the N requirement of future crops.     

Effect of urease and nitrification inhibitors on N transformation, gaseous emissions of ammonia and nitrous oxide, pasture yield and N uptake in grazed pasture system

Nitrogen (N) losses via nitrate (NO₃⁻) leaching, ammonia (NH₃) volatilization and nitrous oxide (N₂O) emissions from grazed pastures in New Zealand are one of the major contributors to environmental degradation. The use of N inhibitors (urease and nitrification inhibitors) may have a role in mitigating these N losses. A one-year field experiment was conducted on a permanent dairy-grazed pasture site at Massey University, Palmerston North, New Zealand to quantify these N losses and to assess the effect of N inhibitors in reducing such losses during May 2005-2006. Cow urine at 600 kg N ha⁻¹ rate with or without urease inhibitor N-(n-butyl) thiophosphoric triamide (nBTPT) or (trade name “Agrotain”) (3 L ha⁻¹), nitrification inhibitor dicyandiamide (DCD) (7 kg ha⁻¹) and the use of double inhibitor (DI) containing a combination of both Agrotain and DCD (3:7) were applied to field plots in autumn, spring and summer. Pasture production, NH₃ and N₂O fluxes, soil mineral N concentrations, microbial biomass C and N, and soil pH were measured following the application of treatments during each season. All measured parameters, except soil microbial biomass C and N, were influenced by the added inhibitors during the three seasons. Agrotain reduced NH₃ emissions over urine alone by 29%, 93% and 31% in autumn, spring and summer respectively but had little effect on N₂O emission. DCD reduced N₂O emission over urine alone by 52%, 39% and 16% in autumn, spring and summer respectively but increased NH₃ emission by 56%, 9% and 17% over urine alone during those three seasons. The double inhibitor reduced NH₃ by 14%, 78% and 9% and N₂O emissions by 37%, 67% and 28% over urine alone in autumn, spring and summer respectively. The double inhibitor also increased pasture dry matter by 10%, 11% and 8% and N uptake by the 17%, 28% and 10% over urine alone during autumn, spring and summer respectively. Changes in soil mineral N and pH suggested a delay in urine-N hydrolysis with Agrotain, and reduced nitrification with DCD. The combination of Agrotain and DCD was more effective in reducing both NH₃ and N₂O emissions, improving pasture production, controlling urea hydrolysis and retaining N in NH₄⁺ form. These results suggest that the combination of both urease and nitrification inhibitors may have the most potential to reduce N losses if losses are associated with urine and improve pasture production in intensively grazed systems.     

Improving dryland cropping system nitrogen balance with no‐tillage and nitrogen fertilization

Studies on N balance due to N inputs and outputs and soil N retention to measure cropping system performance and environmental sustainability are limited due to the complexity of measurements of some parameters. We measured N balance based on N inputs and outputs and soil N retention under dryland agroecosystem affected by cropping system and N fertilization from 2006 to 2011 in the northern Great Plains, USA. Cropping systems were conventional tillage barley (Hordeum vulgaris L.)–fallow (CTB‐F), no‐tillage barley–fallow (NTB‐F), no‐tillage barley–pea (Pisum sativum L.) (NTB‐P), and no‐tillage continuous barley (NTCB). In these cropping systems, N was applied to barley at four rates (0, 40, 80, and 120 kg N ha^?1), but not to pea and fallow. Total N input due to N fertilization, pea N fixation, soil N mineralization, atmospheric N deposition, nonsymbiotic N fixation, and crop seed N and total N output due to grain N removal, denitrification, volatilization, N leaching, gaseous N (NO_x) emissions, surface runoff, and plant senescence were 28–37% greater with NTB‐P and NTCB than CTB‐F and NTB‐F. Total N input and output also increased with increased N rate. Nitrogen accumulation rate at the 0–120 cm soil depth ranged from –32 kg N ha^?1 y^?1 for CTB‐F to 40 kg N ha^?1 y^?1 for NTB‐P and from –22 kg N ha^?1 y^?1 for N rates of 0 kg N ha^?1 to 45 kg N ha^?1 y^?1 for 120 kg N ha^?1. Nitrogen balance ranged from 1 kg N ha^?1 y^?1 for NTB‐P to 74 kg N ha^?1 y^?1 for CTB‐F. Because of increased grain N removal but reduced N loss to the environment and N fertilizer requirement as well as efficient N cycling, NTB‐P with 40 kg N ha^?1 may enhance agronomic performance and environmental sustainability while reducing N inputs compared to other management practices.     

Bio‐inoculant improves nitrogen‐use efficiency and cotton yield in saline soils

Improved nutrient‐use efficiency is important to sustain agricultural production. The goal of our study was to investigate the effects of Azovit® (Azotobacter chroococcum) inoculation of seed with N fertilization on crop yield, nutrient uptake, and N‐use efficiency (NUE) of irrigated cotton (Gossypium hirsutum L. cv. C‐6524) in secondary saline soil under continental climatic conditions of Uzbekistan. A randomized complete block design in a 4 × 2 split‐plot experiment was established in the fall of 2013. The main plot was N fertilization (0, 140, 210, and 280 kg ha^?1) and the subplot was Azovit inoculation. Azovit inoculation consistently increased the seed and lint yields of cotton by 25 and 27.9%, respectively, at 210 kg N ha^?1 compared to the respective control. Azovit with 210 kg N ha^?1 significantly increased the cotton harvest index by 21%, when compared to the control. Likewise, nutrient uptake and NUE of cotton were higher when N (210 kg ha^?1) was applied with Azovit, as compared to other treatment combinations. An extrapolation of the relationship of relative yield vs. N fertilization showed that Azovit at 210 kg N ha^?1 was sufficient to obtain near‐maximum cotton production (90%) with highest NUE, as compared to the respective control. The results suggest that Azovit with 210 kg N ha^?1 produces cotton yield higher and/or comparable with the currently used rates of 280 kg N ha^?1 or higher, suggesting savings of 70 kg N ha^?1 for cotton production in saline soils under continental climatic conditions.     

Catch crop strategy and nitrate leaching following grazed grass-clover

Cultivation of grassland presents a high risk of nitrate leaching. This study aimed to determine if leaching could be reduced by growing spring barley (Hordeum vulgare L.) as a green crop for silage with undersown Italian ryegrass (Lolium multiflorum Lam.) compared with barley grown to maturity with or without an undersown conventional catch crop of perennial ryegrass (Lolium perenne L.). All treatments received 0, 60 or 120 kg of ammonium‐N ha^?1 in cattle slurry. In spring 2003, two grass‐clover fields (3 and 5 years old, respectively, with different management histories) were ploughed. The effects of the treatments on yield and nitrate leaching were determined in the first year, while the residual effects of the treatments were determined in the second year in a crop of spring barley/perennial ryegrass. Nitrate leaching was estimated in selected treatments using soil water samples from ceramic cups. The experiment showed that compared with treatments without catch crop, green barley/Italian ryegrass reduced leaching by 163–320 kg N ha^?1, corresponding to 95–99%, and the perennial ryegrass reduced leaching to between 34 and 86 kg N ha^?1, corresponding to a reduction of 80 and 66%. Also, in the second growing season, leaching following catch crops was reduced compared with the bare soil treatment. It was concluded that the green barley/Italian ryegrass offers advantages not only for the environment but also for farmers, for whom it provides a fodder high in roughage and avoids the difficulties with clover fatigue increasingly experienced by Danish farmers.     

Effects of two contrasting agricultural land‐use practices on nitrogen leaching in a sandy soil of Middle Germany

The objective of this study is to evaluate different agricultural land‐use practices in terms of N leaching and to give recommendations for a sustainable agriculture on sandy soils in Middle Germany. Soil mineral N (N_min) and leachate N were quantified at a sandy soil in N Saxony during 3 years. Two treatments were applied: intensive (I)—using inorganic and organic fertilizer and pesticides, and organic (O)—exclusively using organic fertilizer, legume‐based crop rotation, and no pesticides. Split application of mineral fertilizers did not result in substantial N losses at treatment I. Legumes induced a considerable increase of soil mineral N and particularly of leachate mineral N (N_{min_perc}) at treatment O. High N_{min_perc} concentrations (up to 78 mg N L^–1) were observed during as well as after the cultivation of legumes. These high N_{min_perc} concentrations are the reason why clearly higher N_{min_perc} losses were determined at treatment O (62 kg N ha^–1 y^–1) compared to treatment I (23 kg N ha^–1 y^–1). At both treatments, the quantity of N losses was strongly affected by the precipitation rates. Concentrations and losses of dissolved organic N (DON_perc) were assessed as above average at both treatments. The results suggest that the DON_perc concentration is influenced by precipitation, soil coverage, and organic fertilizers. Higher values were determined in the percolation water of treatment O. The average annual DON_perc losses amounted to 15 kg N ha^–1 at I and to 32 kg N ha^–1 at O. The average monthly percentage of DON_perc losses on the loss of the dissolved total N of percolation water (DTN_perc) ranged between <1% and 55% at O and between 2% and 56% at I. For the whole measuring period of 29 months, the relative amounts of DON_perc of DTN_perc (21% at O and 25% at I) were more or less the same for both treatments. The results show that DON_perc can contribute significantly to the total N loss, confirming the importance to consider this N fraction in N‐leaching studies. It was concluded that at sandy sites, a split application of mineral fertilizers, as applied at treatment I, seems to be more expedient for limiting the N leaching losses than legume‐based crop rotations.     

Nitrate leaching in an organic dairy/crop rotationas affected by organic manure type, livestock densityand crop

Abstract. In dairy farming systems the risk of nitrate leaching is increased by mixed rotations (pasture/arable) and the use of organic manure. We investigated the effect of four organic farming systems with different livestock densities and different types of organic manure on crop yields, nitrate leaching and N balance in an organic dairy/crop rotation (barley–grass-clover–grass-clover–barley/pea–winter wheat–fodder beet) from 1994 to 1998. Nitrate concentrations in soil water extracted by ceramic suction cups ranged from below 1 mg NO₃-N l^?1 in 1^st year grass-clover to 20–50 mg NO₃-N l^?1 in the winter following barley/pea and winter wheat. Peaks of high nitrate concentrations were observed in 2^nd year grass-clover, probably due to urination by grazing cattle. Nitrate leaching was affected by climatic conditions (drainage volume), livestock density and time since ploughing in of grass-clover. No difference in nitrate leaching was observed between the use of slurry alone and farmyard manure from deep litter housing in combination with slurry. Increasing the total-N input to the rotation by 40 kg N ha^?1 year^?1 (from 0.9 to 1.4 livestock units ha^?1) only increased leaching by 6 kg NO₃-N ha^?1. Nitrate leaching was highest in the second winter (after winter wheat) following ploughing in of the grass-clover (61 kg NO₃-N ha^?1). Leaching losses were lowest in 1^st year grass-clover (20 kg NO₃-N ha^?1). Averaged over the four years, nitrate concentration in drainage water was 57 mg l^?1. Minimizing leaching losses requires improved utilization of organic N accumulated in grazed grass-clover pastures. The N balance for the crop rotation as a whole indicated that accumulation of N in soil organic matter in the fields of these systems was small.     

Nitrate leaching loss under annual and perennial pastures with and without lime on a duplex (texture contrast) soil in humid southeastern Australia

Mineral N accumulates in autumn under pastures in southeastern Australia and is at risk of leaching as nitrate during winter. Nitrate leaching loss and soil mineral N concentrations were measured under pastures grazed by sheep on a duplex (texture contrast) soil in southern New South Wales from 1994 to 1996. Legume (Trifolium subterraneum)‐based pastures contained either annual grass (Lolium rigidum) or perennial grasses (Phalaris aquatica and Dactylis glomerata), and had a control (soil pH 4.1 in 0.01 m CaCl₂) or lime treatment (pH 5.5). One of the four replicates was monitored for surface runoff and subsurface flow (the top of the B horizon), and solution NO₃^– concentrations. The soil contained more mineral N in autumn (64–133 kg N ha^?1 to 120 cm) than in spring (51–96 kg N ha^?1), with NO₃^– comprising 70–77%. No NO₃^– leached in 1994 (475 mm rainfall). In 1995 (697 mm rainfall) and 1996 (666 mm rainfall), the solution at 20 cm depth and subsurface flow contained 20–50 mg N l^?1 as NO₃^– initially but < 1 mg N l^?1 by spring. Nitrate‐N concentrations at 120 cm ranged between 2 and 22 mg N l^?1 during winter. Losses of NO₃^– were small in surface runoff (0–2 kg N ha^?1 year^?1). In 1995, 9–19 kg N ha^?1 was lost in subsurface flow. Deep drainage losses were 3–12 kg N ha^?1 in 1995 and 4–10 kg N ha^?1 in 1996, with the most loss occurring under limed annual pasture. Averaged over 3 years, N losses were 9 and 15 kg N ha^?1 year^?1 under control and limed annual pastures, respectively, and 6 and 8 kg N ha^?1 year^?1 under control and limed perennial pastures. Nitrate losses in the wet year of 1995 were 22, 33, 13 and 19 kg N ha^?1 under the four respective pastures. The increased loss of N caused by liming was of a similar amount to the decreased N loss by maintaining perennial pasture as distinct from an annual pasture.     

Nitrate leaching from the Broadbalk Wheat Experiment, Rothamsted, UK, as influenced by fertilizer and manure inputs and the weather

Abstract. Nitrate leaching was measured over the eight drainage seasons spanning the nine years from 1990–1998 on the 157‐year old Broadbalk Experiment at Rothamsted, UK. The weather pattern of two dry, three wet and three dry years was the dominant factor controlling nitrogen (N) loss. Both the concentration of nitrate in the drainage waters and the amount of N leached increased with the amount of N applied, mostly because of long‐term, differential increases in soil organic matter and mineralization. On average, losses of N by leaching were 30 kg ha^?1yr^?1 when no more than the optimum N application was applied and were typical of amounts leached from arable land in the UK. Losses increased significantly in both amounts and as the percentage of N applied for supra‐optimal applications of N and from autumn‐applied farmyard manure (FYM). Extra spring‐applied fertilizer was very effective at increasing yields on plots given FYM in the autumn but at the expense of leaching losses three times those from optimum fertilizer N applications. Losses increased after potatoes because they left significant amounts of mineral N in the soil, and decreased after forage maize because it used applied N more effectively. Losses measured 120 years ago from identical treatments were 74% greater than current losses because of today's larger yields and more efficient varieties and management practices. Average concentrations of nitrate in drainage waters did not exceed the EU limit of 11.3 mg NO₃‐N l^?1 until supra‐optimal amounts of N fertilizer (>150–200 kg ha^?1yr^?1) were applied in spring or FYM was applied in autumn. However some drainage waters from all plots, even those that have not received fertilizer for >150 years, exceeded the limit when rain followed a dry summer and autumn. Nitrate leaching into waters will remain a problem for profitable arable farming in the drier parts of Eastern England and Europe despite increased N use efficiency.     

Alysimeter study of the effects of a ryegrass catch crop, during a winter wheat/maize rotation, on nitrate leaching and on the following crop

The effects of an intercrop catch crop (Italian ryegrass) on (i) the amounts and concentrations of nitrate leached during the autumn and winter intercrop period, and (ii) the following crop, were examined in a lysimeter experiment and compared with that from a bare fallow treatment. The catch crop was grown in a winter wheat/maize rotation, after harvest of the wheat, and incorporated into the soil before sowing the maize. A calcium and potassium nitrate fertilizer labelled with ¹⁵N (200 kg N ha^?1; 9.35 atom per cent excess) was applied to the winter wheat in spring. Total N uptake by the winter wheat was 154 kg ha^?1 and the recovery of fertilizer-derived N (labelled with ¹⁵N) was 60%. The catch crop (grown without further addition of N) yielded 3.8t ha^?1 herbage dry matter, containing 43 kg N ha^?1, of which 4.1 % was derived from the ¹⁵N-labelled fertilizer. Two-hundred kg unlabelled N ha^?1 was applied to the maize crop. During the intercrop period the nitrate concentration in water draining from the bare fallow lysimeters reached 68 mg N1^?1, with an average of 40 mg N1^?1. With the catch crop, it declined rapidly, from 41 mg N I^?1 to 0.25 mg N I^?1, at the end of ryegrass growth. Over this period, 110 kg N ha^?1 was leached under bare fallow, compared with 40 kg N ha^?1 under the catch crop. ¹⁵N-labelled nitrate was detected in the first drainage water collected in autumn, 5 months after the spring application. The quantity of fertilizer-N that was leached during this winter period was greater under bare fallow (18.7% of applied N) than when a catch crop was grown (7.1 %). In both treatments, labelled fertilizer-N contributed about 34% of the total N lost during this period. With the ryegrass catch crop incorporated at the time of seedbed preparation in spring, the subsequent maize grain-yield was lowered by an average of 13%. Total N-uptake by the maize sown following bare fallow was 224 kg N ha^?1, compared with 180 kg ha^?1 with prior incorporation of ryegrass; the corresponding values for uptake of residual labelled N were 3% (bare fallow) and 2% (ryegrass) of the initial application. Following the maize harvest, where ryegrass was incorporated, 22.7% of the previous year's labelled fertilizer addition was present in an organic form on the top 30 cm of lysimeter soil. This compares with 15.7% for the bare fallow intercropping treatment. Tracer analyses showed overall recoveries of labelled N of 91.7% for the winter wheat/ ryegrass/maize rotation and 97% for the winter wheat/bare fallow/maize rotation. The study clearly demonstrated the ecological importance of a catch crop in reducing N-leaching as well as its efficient use of fertilizer in the plant-soil system from this particular rotation. However, the fate of the organic N in the ploughed-down catch crop is uncertain and problems were encountered in establishing the next crop of maize.     

A Lysimeter Study to Investigate the Effect of Dairy Effluent and Urea on Cattle Urine n Losses, Plant Uptake and Soil Retention

Loss of nitrate (NO₃ ^?) from grazing land is a major cause of surface and groundwater contamination. These losses increase when N sources such as fertilizer are applied to grazing land. The objectives of this work were to (1) study the impact of dairy effluent (DE) or urea on N losses and plant uptake when DE or urea was applied with and without cattle urine and; (2) determine the effect of organic C rich DE on the fate of urine N. The experiment was conducted using lysimeters that contained Templeton sandy loam soil extracted from a pasture in New Zealand. Application of DE resulted in significantly less (p < 0.05) NO₃ ^? leaching compared with urea in the first year, but not in the second year. Differences between years could be attributed to the comparatively lower C:N ratio of applied DE in the second year, causing relatively greater N mineralization and greater NO₃ ^? leaching. Differences could also be due to cumulative effects of DE (first year applied) on second year NO₃ ^? leaching. Total annual pasture N uptake was similar for DE and urea treatments. During the first year, the average NO₃ ^? concentration was lower when DE was combined with urine compared to urine alone, but not in the second year. The combination of DE with urine resulted in significantly greater (p < 0.01) annual pasture N uptake compared with the urine alone treatment in both years. Urine plus urea resulted in the greatest leaching losses in both years, but its impact on pasture N uptake was mixed. The total leaching loss of N from urine plus DE (90 kg N ha^?1) was similar to urine alone (77 kg N ha^?1) in the second year. Likewise, the annual percentage of ¹⁵N recovered in the leachate from urine plus DE (9%) was not significantly different from urine alone (6%). However, ¹⁵N recoveries revealed that the contribution of urine N to NO₃ ^? leaching was greater when urine was combined with DE (98.8%) compared to urine alone (83%). The greater NO₃ ^? leaching from urine when combined with DE could be a result of greater nitrification due to the low C:N ratio of DE. Additionally, the annual percentage of urine N uptake by the pasture from urine plus DE (29%) was significantly less than from urine alone (39%) (p < 0.01). The application of organic C rich DE had no significant effect on soil N retention or denitrification when combined with urine.     

Leaching of dissolved organic carbon and nitrogen under cotton farming systems in a Vertisol

Leaching with deep drainage is one of the loss pathways of carbon (C) and nitrogen (N) in cropping fields. However, field studies in irrigated row cropping systems are sparse. A 3‐year investigation on C and N leaching associated with deep drainage was overlaid on a long‐term experiment on tillage practices and crop rotations in Australia. The treatments included cotton (Gossypium hirsutum L.) monoculture and cotton–wheat (Triticum aestivum L.) or maize (Zea maize L.) rotations with maximum or minimum tillage. The deep drainage C and N concentrations at 0.6 and 1.2 m depth were measured after furrow irrigation with ceramic cup samplers during the 2014–15, 2015–16 and 2016–17 cotton seasons. Pre‐planting dissolved organic carbon (DOC) concentration in soil at 0.6–1.2 m depth during 2016–17 was 64 mg kg^?1 for maximum tilled cotton monoculture, 36 mg kg^?1 for minimum tilled cotton monoculture and 39 mg kg^?1 for cotton–wheat, and in maize and cotton subplots 51 and 41 mg kg^?1, respectively. Post‐harvest DOC values in soil were similar in all treatments (average of 32 mg DOC kg^?1). Total organic carbon (TOC) losses in deep drainage were equal to 2%–30% of TOC gained in irrigation water. Oxidized N losses in deep drainage ranged from 0.7% to 12% of applied N (260 kg ha^?1). NOx‐N concentrations in leachate under maize systems (20 mg L^?1) were up to 73% lower than those in cotton systems (75 mg L^?1). Maize sown in rotation with cotton can improve cotton yield, reduce N leaching and improve N use efficiency of subsequent cotton.     

Inoculant plant growth-promoting microorganisms enhance utilisation of urea-N and grain yield of paddy rice in southern Vietnam

Field experiments were conducted during successive rainy seasons in 2006 in the Chau Thanh district of southern Vietnam to evaluate the effects of an inoculant plant growth promoter product called “BioGro” and N fertiliser rates on yield and N and P nutrition of rice. The results indicated that inoculation with BioGro, containing a pseudomonad, two bacilli and a soil yeast, significantly increased grain and straw yields and total N uptake in both seasons, as well as grain quality in terms of percentage N. Nitrogen fertilisation increased grain and straw yields as well as total N and P uptakes significantly in both cropping seasons. The estimated grain yield response to added N was quadratic in nature with and without added BioGro. In the first crop, BioGro out-yielded the control up to 90 kg urea N ha^?1 whilst in the second season the beneficial effect of BioGro was observed up to 120 kg urea N ha^?1, indicating either an interaction of the inoculant with higher yielding seasonal conditions or a cumulative effect of BioGro application. In the first season, the estimated N rate for maximum grain yield was 103 kg N ha^?1 with BioGro while it was 143 kg N ha^?1 without BioGro. The maximum estimated grain yields were 3.21 and 3.18 t ha^?1 with and without BioGro, respectively. This information indicates that BioGro was able to save 40 kg N ha^?1 with an additional rice yield of 30 kg ha^?1 in the season. In the second rainy season, the estimated N rates for maximum grain yields were 94 and 97 kg N ha^?1 with and without BioGro, respectively. The estimated maximum grain yields were 3.49 and 3.25 t ha^?1 with and without BioGro, respectively. The two seasons’ combined results indicate that application of BioGro improved the efficiency of N use by rice significantly, saving 43 kg N ha^?1 with an additional rice yield of 270 kg ha^?1 in two consecutive seasons at the experimental site. The extra efficiency was shown by the fact that the same yield of rice was obtained with about 40 and 60 kg less fertiliser-N that the maximum yields with urea alone in the two successive harvests on the same plots.     

The potential of using alternative pastures,forage crops and gibberellic acid to mitigate nitrous oxide emissions

Purpose

In grazed pastures, nitrous oxide (N₂O), a powerful greenhouse gas and an ozone depletion substance, is mostly emitted from animal excreta, particularly animal urine-N returned to the soil during grazing. We conducted a series of four field lysimeter and plot experiments to assess the potential of using gibberellic acid (GA) and/or alternative pastures or forage crops to mitigate N₂O emissions from outdoor dairy farming systems.

Materials and methods

Pasture and forage plants assessed in the experiments included Italian ryegrass (Lolium multiflorum L.), lucerne (Medicago sativa L.), diverse pastures (including plantain (Plantago lanceolata L.), chicory (Cichorium intybus L.), perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.)), fodder beet (Beta vulgaris L.), kale (Brassica oleracea L.), as well as the standard perennial ryegrass and white clover (RG/WC) pastures. N₂O was determined using a standard static chamber method in the field either on top of lysimeters or field plots.

Results and discussion

The results showed that the application of GA to urine-treated lysimeters with Italian ryegrass, lucerne or RG/WC pastures did not result in lower N₂O emissions. However, the use of diverse pastures which included plantain with a lower urine-N loading rate at about 500 kg N ha^?1 significantly decreased N₂O emissions by 46 % compared with standard RG/WC with a urine-N loading rate at 700 kg N ha^?1. However, when urine-N was applied at the same rates (at 500 or 700 kg N ha^?1), the N₂O emissions were similar between the diverse and the standard RG/WC pastures. This would indicate that it is the N-loading rate in the urine from the different pastures that determines the N₂O emissions from different pastures or forages, rather than the plants per se. The N₂O emissions from cow urine from fodder beet were 39 % lower than from kale with the same urine-N application rate (300 kg N ha^?1).

Conclusions

These results suggest that N₂O emissions can potentially be reduced by incorporating diverse pastures and fodder beet into the grazed pasture farm system. Further studies on possible mechanisms for the lower N₂O emissions from the different pastures or forages would be useful.