Labdane-type diterpenes and flavones from Dodonaea viscosa

A new labdane diterpenoid, 2,18-dihydroxylabda-7,13(E)-dien-15-oic acid (1), together with two known labdane diterpenes (3, 4), a new flavone, 5,7-dihydroxy-3,6,4'-trimethoxy-3'-(4-hydroxy-3-methyl-but-2-enyl)flavone (2) with three known flavones (5-7) were isolated from the aerial parts of Dodonaea viscosa. Their structures were determined by extensive analysis of spectroscopic data (1D and 2D NMR, MS) and by comparison with literature data. The anti-inflammatory activity of five compounds (1-5) was evaluated with a flow cytometry TNF-α secretion assay on human THP-1 cell line.     

A new flavonoid glycoside from the leaf of Cephalotaxus koreana

A new flavone glycoside, apigenin 5-O-alpha-L-rhamnopyranosyl-(1-->3)-beta-D-glucopyranoside (1), along with four known flavonol glycosides (2-5), were isolated from the leaf of Cephalotaxus koreana. The new glycoside 1 showed inhibitory activity in superoxide radical scavenging assay with IC(50) value of 13.0 microM, while it showed weak activity in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. Compounds 2-5 exhibited antioxidant activity in scavenging DPPH and superoxide radicals with IC(50) values ranging from 5.7 to 22.3 microM.     

Anti-HBV active flavone glucosides from Euphorbia humifusa Willd.

Thirteen flavone glucosides from the herb of Euphorbia humifusa were isolated and elucidated. Among them, five compounds including apigenin-7-O-β-d-glucopyranoside (2), apigenin-7-O-(6′′-O-galloyl)-β-d-glucopyranoside (3), luteolin-7-O-β-d-glucopyranoside (7), luteolin-7-O-(6′′-O-trans-feruloyl)-β-d-glucopyranoside (8) and luteolin-7-O-(6′′-O-coumaroyl)-β-d-glucopyranoside (9) showed anti-HBV activity in vitro. The structure–activity relationship showed that the parent structure was closely relevant to the anti-HBV activity of these compounds (agigenin > luteolin > quercetin). It was found that the number of glucoside in the structure may significantly influence their activities (flavone monoglucoside > flavone diglucoside) and cytotoxicity (flavone > flavone monoglucoside > flavone diglucoside). In addition, the substitution of acyl group on glucoside may be important to keep the anti-HBV activities of these compounds (galloyl > feruloyl > coumaroyl).     

Potent antiviral flavone glycosides from Ficus benjamina leaves

Crude ethanol extracts from Ficus benjamina leaves strongly inhibit Herpes Simplex Virus 1 and 2 (HSV-1/2) as well as Varicella Zoster Virus (VZV) cell infection in vitro. Bioassay-guided fractionation of the crude extract demonstrated that the most efficient inhibition of HSV-1 and HSV-2 was obtained with the flavonoid fraction. The present study was aimed to further isolate, purify and identify substances with potent antiviral activity from the flavonoid fraction of F. benjamina extracts. Flavonoids were collected from the leaf ethanol extracts through repeated purification procedure and HPLC analysis. The antiviral activity of each substance was then evaluated in cell culture. Three known flavone glycosides, (1) quercetin 3-O-rutinoside, (2) kaempferol 3-O-rutinoside and (3) kaempferol 3-O-robinobioside, showing highest antiviral efficiency were selected and their structure was determined by spectroscopic analyses including NMR and mass spectrometry (MS). These three flavones were highly effective against HSV-1 reaching a selectivity index (SI) of 266, 100 and 666 for compound 1, 2 and 3, respectively, while the SI of their aglycons, quercetin and kaempferol amounted only in 7.1 and 3.2, respectively. Kaempferol 3-O-robinobioside showed similar SI to that of acyclovir (ACV), the standard anti-HSV drug. Although highly effective against HSV-1 and HSV-2, these flavone glycosides did not show any significant activity against VZV.     

Iridoid, phenylethanoid and flavonoid glycosides from Sideritis trojana

From the MeOH extract of Sideritis trojana, a new iridoid glycoside, 10-O-(E)-feruloylmelittoside (1) was obtained in addition to four known iridoid glycosides [melittoside (2), 10-O-(E)-p-coumaroylmelittoside (3), stachysosides E (4) and G (5)]. Moreover, five phenylethanoid glycosides [verbascoside (6), isoacteoside (7), lamalboside (8), leonoside A (9), isolavandulifolioside (10), three flavone glycosides (isoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (11), 4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (12), 3'-hydroxy-4'-O-methyisoscutellarein 7-O-[6'-O-acetyl-β-allopyranosyl-(1→2)]-β-glucopyranoside (13) and a benzylalcohol derivative (di-O-methylcrenatin) were obtained and identified. The structures were elucidated on the basis of NMR and HRMS data. All compounds were tested for their antioxidant activity by in vitro TEAC assay and some of them exhibited moderate activity (0.97-1.44 mM) when compared with the reference compound (quercetin 1.86 mM). Glycosides 6-13, the most active compounds in the TEAC assay, were also tested by flow cytometry to evaluate their ability to affect the levels of reactive oxygen species (ROS) in human prostate cancer cells (PC3).     

Flavonoids from Calicotome villosa

Two new acylated flavone glycosides, chrysin 7-(6"-O-acetyl)-O-beta-D-glucopyranoside (1) and chrysin 7-(4"-O-acetyl)-O-beta-D-glucopyranoside (2) were isolated from the aerial parts of Calicotome villosa, along with 17 known flavonoids and one triterpene.     

两种长春花体内黄酮含量的研究

对野生及露地栽培生境中生长的红长春花和白长春花不同部位中总黄酮含量进行比较研究,研究结果显示:1)长春花各部位中总黄酮含量从高到低为果(含种子)、叶片、花、茎、根;2)野生白长春花体内总黄酮含量低于栽培白长春花;3)野生红长春花体内总黄酮含量与露地栽培红长春花的含量相接近;4)红长春花体内总黄酮含量高于白长春花;5)两种长春花在茎和叶片在不同高度上的总黄酮含量有所差异。长春花体内黄酮物质的这种分布特点将为下一步园林花卉的引种驯化工作提供理论支持。     

Artoindonesianin L, a new prenylated flavone with cytotoxic activity from Artocarpus rotunda.

A new prenylated flavone, named artoindonesianin L (1), was isolated from Artocarpus rotunda (Hout) Panzer (Moraceae). Its structure was elucidated as on the basis of spectroscopic evidence. Along with this new compound, four known phenolic compounds were also isolated from this plant and identified as artonins M (2) and E (3), cycloartobiloxanthone (4) and artonin O (5). All these compounds showed significant cytotoxicity against murine P388 leukemia cells.     

小叶买麻藤种子营养及药用成分分析

正小叶买麻藤(Gnetum parvifolium(Warb.)C.Y.Cheng)是一种珍贵的药用裸子植物,主要分布在热带、亚热带地区,作为珍稀濒危物种,已被收入中国物种红色名录[1]。作为我国传统中药,小叶买麻藤具有降血压、祛除风湿、止血凉血等功效[2-5]。除此之外,买麻藤属植物中某些种的叶片是非洲等国家重要的出口蔬菜[6],并且种子煮熟后可以食用。小叶买麻藤     

Anti-inflammatory activity of sulfate-containing phenolic compounds isolated from the leaves of Myrica rubra

Three sulfated phenolic compounds, juglanin B (11R)-O-sulfate (1), myricetin 3´-O-sulfate (2), and ampelopsin 3´-O-sulfate (3), were isolated from the leaves of Myrica rubra. Compound 1 was a new sulfated lignan, 2 was a new sulfated flavone, and 3 was a known sulfated flavone. The structures of the new compounds (1 and 2) were determined by acid hydrolysis and spectroscopic methods, including IR, FAB-MS, 1D and 2D NMR. The inhibitory activities of compounds 1–3 and their hydrolysates (1a–3a) against LPS-induced cytokine (TNF-α, IL-1β, and IL-6) production in macrophage RAW 264.7 cells were evaluated. The 2 new compounds (1 and 2) and their aglycones (1a and 2a) significantly reduced LPS-induced expression of iNOS and COX-2 proteins.     

Sesquiterpene lactones from the aerial parts of Inula oculus-christi

Pulchellin E (1) and gaillardin (2) were isolated from the aerial parts of Inula oculus-christi, along with the flavone hispidulin. The 13C-NMR chemical shifts of 1 and 2 are reported.     

Artoindonesianin L, a new prenylated flavone with cytotoxic activity from Artocarpus rotunda

A new prenylated flavone, named artoindonesianin L (1), was isolated from Artocarpus rotunda (Hout) Panzer (Moraceae). Its structure was elucidated as on the basis of spectroscopic evidence. Along with this new compound, four known phenolic compounds were also isolated from this plant and identified as artonins M (2) and E (3), cycloartobiloxanthone (4) and artonin O (5). All these compounds showed significant cytotoxicity against murine P388 leukemia cells.     

Artoindonesianin P,a new prenylated flavone with cytotoxic activity from Artocarpus lanceifolius

A new prenylated flavone, named artoindonesianin P (1), was isolated from the tree bark of Artocarpus lanceifolius, together with three known related compounds, artobiloxanthone (2), cycloartobiloxanthone (3) and artonol B (4). The structure of artoindonesianin P 1 was determined on the basis of spectral evidence (MS, 1H and 13C NMR) and by comparison with known related compounds. Compounds 1-4 exhibited significant cytotoxicity against murine P388 leukemia cells.     

湘葛一号葛根活性物质提取制备葛根功能饮品研究

以葛根为原料,以葛根素为主要评价指标,研究了葛根中各主要活性成分的含量、葛根黄酮的制备、葛根黄酮饮品的稳定性及极端条件的破坏性、葛根黄酮饮品口感及感官指标、葛根黄酮饮品生产工艺等。结果表明:与泰国葛根、粉葛等相比,湘葛一号葛根中葛根素等活性成分含量明显较高。不同浓度乙醇提取葛根总黄酮,以65%乙醇提取效果最好。湘葛一号天然葛根饮品的制备过程中,低浓度(21.6 mg/500 mL)、中浓度(216 mg/500 mL)饮品在不同温度条件下的葛根黄酮均无明显变化,含量稳定;高浓度(360 mg/500 mL)饮品葛根黄酮含量均有不同程度减少,稳定性稍差;中、高浓度饮品葛根黄酮高温易氧化变色,低温易沉淀析出,故低浓度饮品稳定性最佳。在各浓度葛根饮品中,每500 mL含葛根素100 mg及以下的葛根饮品颜色透明清亮;每500 mL含葛根素2.16 mg等低浓度的饮品与纯净水无明显口感差异,21.6 mg级别的饮品口感微苦,并有一种清凉感,适合饮用。     

A new prenylated arylnaphthalene lignan from Haplophyllum myrtifolium

A novel prenylated arylnaphthalene lignan, 7-O-(3-methyl-2-butenyl)isodaurinol, was isolated from Haplophyllum myrtifolium and identified on the basis of detailed spectral analyses, including 2D-NMR spectrometry. The known furoquinoline alkaloids, dictamnine, robustine, gamma-fagarine and skimmianine, the aryltetralin lignan (-)-1beta-polygamain and the flavone chrysosplenetin were isolated from the same source.     

中国沙棘果实化学成分初步研究

采用系统预试法,对中国沙棘果皮渣所含化学成分进行了定性检验,并测定了油脂和黄酮含量。结果表明,中国沙棘果实含有鞣质,有机酸,酚类,黄酮类、挥发油及油脂,并且油脂和黄酮含量较高。     

A new flavone glycoside from Centaurea furfuracea

A new flavone glycoside, hispidulin 7-O-methylglucuronide (1) has been isolated from the aerial part of Centaurea furfuracea.     

A new flavonoid from the aerial part of Thalictrum atriplex

A new flavone glycoside, identified as kaempferol 3-O-[3'"-acetyl-alpha-L-arabinopyranosyl-(1'"-6")]-beta-D-gluco pyranoside (1), has been isolated from the aerial part of Thalictrum atriplex.     

不同地域银杏叶中总黄酮的测定及开发价值评价

分光光度法测定了黄河流域、长江流域１６个省同一时期银杏叶中总黄酮的含量,测试结果表明,黄酮含量生产地域有密切的相关性。据此,本文对各地域银杏叶的开发价值进行了评价,并对银杏叶的开发利用提出了建设性的建议,具有一定的宏观指导意义,为合理开发利用好我国这一特有资源提供可靠的理论依据。     

竹叶黄酮的生理功能研究进展

竹叶含有丰富的黄酮类化合物,其提取物是我国新开发的一种植物类黄酮制剂,阐述其优良的抗自由基、抗氧化、抗衰老、抗菌、抗病毒及保护心脑血管等生物学功效,展望竹叶黄酮的应用前景。