Comparison of target breeding weight and breeding date for replacement beef heifers and effects on subsequent reproduction and calf performance

A 3-yr study was conducted with spring-born heifers (n = 240) to determine the effects of developing heifers to either 55 or 60% of mature BW at breeding on reproduction and calf production responses. A concurrent study was also conducted with summer-born heifers (n = 146) to examine effects of breeding heifers with the mature cow herd or 1 mo earlier on reproduction and calf production variables. Spring-born crossbred heifer calves were weaned and developed on two different levels of nutrition to achieve the desired prebreeding BW. Summer-born heifers were developed to similar target breeding BW (60% of mature BW) to begin calving either 1 mo before (May) or at the same time as the mature cowherd (June). Blood samples were taken before breeding to determine differences in estrous cyclicity. Pregnancy rates through the fourth pregnancy were determined. Cow and calf production variables were evaluated through the third gestation. Spring-born heifers reached 53 or 58% of mature BW at breeding and had similar reproduction and first calf production traits between the two, groups. Calving difficulty with the second calf was greater (P < 0.05) for heifers developed to 58% of mature BW at breeding. Subsequent second calf weaning weight and ADG were decreased (P < 0.05) for heifers developed to 58% of mature BW at breeding. Feed costs were $22/heifer less for heifers developed to 53% of mature BW. Summer-born first-calf heifers calving in June had less (P < 0.01) calving difficulty than did heifers calving in May; however, calf birth weights were similar. Breeding summer-born heifers 1 mo before the cowherd did not influence pregnancy rates over three calf crops; however, first calf adjusted weaning weights and ADG were greater for calves born earlier. Development costs were $11/heifer more for heifers developed to calve in May vs. June. Developing spring-born heifers to 53% of mature BW did not adversely affect reproduction or calf production traits compared with developing heifers to 58% of mature BW, and it decreased development costs. Breeding summer-born heifers before the cowherd increased heifer development costs, increased calving difficulty, and improved calf performance, but had no effect on pregnancy rates.     

Effects of dam nutrition on growth and reproductive performance of heifer calves

A 3-yr study was conducted with heifers (n = 170) whose dams were used in a 2 x 2 factorial arrangement of treatments to determine the effects of late gestation (LG) or early lactation (EL) dam nutrition on subsequent heifer growth and reproduction. In LG, cows received 0.45 kg/d of a 42% CP supplement (PS) or no supplement (NS) while grazing dormant Sandhills range. During EL, cows from each late gestational treatment were fed cool-season grass hay or grazed sub-irrigated meadow. Cows were managed as a single herd for the remainder of the year. Birth date and birth weight of heifer calves were not affected (P > 0.10) by dam nutrition. Meadow grazing and PS increased (P = 0.02; P = 0.07) heifer 205-d BW vs. feeding hay and NS, respectively. Weight at prebreeding and pregnancy diagnosis were greater (P < 0.04) for heifers from PS dams but were unaffected by EL nutrition (P > 0.10). There was no effect (P > 0.10) of LG or EL dam nutrition on age at puberty or the percentage of heifers cycling before breeding. There was no difference (P > 0.10) in pregnancy rates due to EL treatment. Pregnancy rates were greater (P = 0.05) for heifers from PS dams, and a greater proportion (P = 0.005) of heifers from PS dams calved in the first 21 d of the heifers' first calving season. Nutrition of the dams did not influence (P < 0.10) heifers' average calving date, calving difficulty, and calf birth weight during the initial calving season. Weight at the beginning of the second breeding season was greater (P = 0.005) for heifers from PS dams but was not affected by maternal nutrition during EL (P > 0.10). Dam nutrition did not affect (P > 0.10) heifer ADG or G:F ratio. Heifers from PS dams had greater DMI (P = 0.09) and residual feed intake (P = 0.07) than heifers from NS cows if their dams were fed hay during EL but not if their dams grazed meadows. Heifers born to PS cows were heavier at weaning, prebreeding, first pregnancy diagnosis, and before their second breeding season. Heifers from cows that grazed meadows during EL were heavier at weaning but not postweaning. Despite similar ages at puberty and similar proportions of heifers cycling before the breeding season, a greater proportion of heifers from PS dams calved in the first 21 d of the heifers' first calving season, and pregnancy rates were greater compared with heifers from NS dams. Collectively, these results provide evidence of a fetal programming effect on heifer postweaning BW and fertility.     

Heifer development systems: a comparison of grazing winter range or corn residue

Two experiments at 2 Nebraska locations evaluated effects of heifer development system on growth and pregnancy rate. In Exp. 1, heifers (n=270, BW=225 ± 2 kg) grazed winter Sandhills range (WR) or west central Nebraska corn residue (CR) with a supplement (0.45 kg/animal; 31% CP; 80 mg·animal(-1)·d(-1) of monensin). In Exp. 2, heifers (n=180, BW = 262 ± 3 kg) grazed eastern Nebraska WR or CR with a supplement (0.45 to 0.90 kg/d; 31% CP; 80 to 160 mg·animal(-1)·d(-1) of monensin). The CR heifers tended to have less (P=0.10) ADG compared with WR heifers before breeding in Exp. 1; however, prebreeding ADG was similar (P=0.77) in Exp. 2. Prebreeding BW, percentage of mature BW at breeding, and pregnancy determination BW were similar (P ≥ 0.14) for CR and WR in both experiments. Percentage of heifers pubertal at breeding, AI conception, and AI pregnancy rate (Exp. 2) and final pregnancy rate in both experiments were also similar (P ≥ 0.27) for CR and WR heifers. Precalving BW, percentage of calves born in the first 21 d, calf birth date, calf birth BW, and dystocia score were all similar (P ≥ 0.21) for CR and WR heifers in both experiments. Cow BW at weaning, calf weaning BW, adjusted 205-d calf BW, and second season pregnancy rates were not affected (P ≥ 0.16) by treatment. Heifer development system did not affect (P ≥ 0.56) the cost of producing 1 pregnant heifer in Exp. 1 or 2. Development on CR may reduce ADG before breeding, but did not affect pregnancy rate. Heifer development using CR or WR postweaning resulted in similar reproductive performance and development cost.     

Beef heifer development within three calving systems

A 3-yr study was conducted to evaluate the effects of calving system, weaning age, and postweaning management on growth and reproduction in beef heifers. Heifer calves (n = 676) born in late winter (average birth date = February 7 +/- 9 d) or early spring (average birth date April 3 +/- 10 d) were weaned at 190 or 240 d of age, and heifers born in late spring (average birth date May 29 +/- 10 d) were weaned at 140 or 190 d of age. Heifers were managed to be first exposed to breeding at approximately 14 mo of age. After weaning, the calves were randomly assigned to treatments. Heifers on the constant gain treatment were fed a corn silage- and hay-based diet. Heifers on delayed gain treatments were placed on pasture but were fed grass hay or a supplement, or both, depending on the forage conditions. Three months before their respective breeding seasons, delayed gain heifers were moved to drylot and fed a corn silage- and barley-based diet (late winter or early spring) or moved to spring rangeland (late spring). The data were analyzed using mixed model procedures with calving system, weaning age, and postweaning management options creating 12 treatments. Average daily gain was 0.36 +/- 0.05 (SED) kg/d less (P < 0.001) for delayed gain heifers during the initial phase, whereas these heifers gained 0.44 +/- 0.03 kg/d more (P < 0.001) than constant gain heifers during the last 90 d before breeding. Body weights at the beginning of the breeding season did not differ (P = 0.97) between constant gain and delayed gain heifers but were affected by calving system and weaning age, reflecting some of the differences in initial BW. Prebreeding BW for heifers weaned at 190 d of age were 36 +/- 6.4 kg heavier (P < 0.001) for those born in late winter and early spring compared with late spring and were 388, 372, and 330 kg for heifers weaned in October at 240, 190, or 140 d of age (linear effect, P < 0.001). The proportion of heifers exhibiting luteal activity at the beginning of the breeding season was not affected (P = 0.57) by treatment. Approximately half of the heifers were randomly selected for breeding. Treatment had no effect (P = 0.64) on pregnancy rates. In conclusion, heifers from varied calving systems and weaning strategies can be raised to breeding using either constant or delayed gain strategies without affecting the percentage of heifers cycling at the beginning of the breeding season. These results suggest that producers have multiple options for management of heifer calves within differing calving systems.     

Heifer development systems: dry-lot feeding compared with grazing dormant winter forage

Two hundred ninety-nine Angus-based, nulliparous heifers (253 ± 2 kg initial BW) from 3 production years were utilized to compare traditional postweaning dry lot (DL) development with a more extensive winter grazing system utilizing a combination of corn residue and winter range (EXT). Heifers developed in the DL were offered a common diet after the weaning period for 208 d in yr 1, 194 d in yr 2, and 150 d in yr 3 until breeding. Heifers developed in EXT grazed corn residue for 135 d in yr 1, 106 d in yr 2, and 91 d in yr 3, and then fed in the DL until breeding (yr 1) or grazed dormant winter grass for approximately 60 d before being fed in the DL (yr 2 and 3). All 3 years, heifers were estrus synchronized, with timed AI performed in yr 1. In yr 2 and 3, estrus was detected and those detected in estrus were artificially inseminated approximately 12 h later. Heifers were exposed to bulls 10 d after the last AI for 60 d while grazing summer pasture. During the winter grazing period, EXT heifers gained less (P = 0.01) BW than DL heifers and EXT heifers had lighter (P = 0.02) BW at breeding. Fewer (P < 0.01) EXT heifers reached puberty before breeding. Conception to AI was not different (P = 0.23); however, AI pregnancy rate tended (P = 0.08) to be less in EXT heifers. Final pregnancy rates were not different (P = 0.38) between treatment groups. Although EXT heifers had lighter (P = 0.02) BW at pregnancy diagnosis; however, they did compensate with greater (P = 0.05) ADG after breeding, resulting in similar (P = 0.22) precalving BW. Winter development system did not influence (P > 0.10) percentage of calving in the first 21 d, calf birth date, and calf birth BW, or dystocia score. Pregnancy rate after the second breeding season was not different (P = 0.56) between treatments. Heifer development using extended winter grazing reduced (P < 0.01) the cost of producing a pregnant heifer by $45 compared with DL.     

Reproductive performance and profitability of heifers fed to weigh 272 or 318 kg at the start of the first breeding season

Reproductive performance and weaning weight of the first calf was determined in 221 Brahman crossbred heifers fed to weigh either 272 (TW1) or 318 kg (TW2) at the start of their first breeding season (target weight). Heifers were divided into light- (below average) and heavyweight (above average) groups on the basis of initial weight. Within each target weight, heifers were fed in three lots. One lot contained lightweight heifers, the second contained heavy heifers and the third was composed of one-half heavy- and one-half lightweight heifers. Heifers were fed for 200 d before the start of the first breeding season. More heifers in TW2 showed estrus and became pregnant in the first 20 d of the breeding season and more were pregnant at the end of the first breeding season. These same differences in reproductive performance were also noted the second year. Each heifer exposed in TW2 weaned 43.4 kg more calf than those in TW1. An average heifer in TW2 was fed 220 kg more corn and 100 kg less hay than a corresponding heifer in TW1. Estrus and pregnancy rate for lightweight heifers in TW1 and TW2 were not improved by sorting and feeding them separately.     

Production performance of beef cows raised on three different nutritionally controlled heifer development programs

The objective of this study was to determine primiparous heifer performance following three different heifer development strategies that were the result of timed nutrient limitation. Two hundred eighty-two spring-born MARC III heifers were weaned at 203+/-1 d of age and 205+/-1 kg BW. The experiment was conducted on two calf crops with 120 heifers born in 1996 and 162 heifers born in 1997. Treatments consisted of different quantities of the same diet being offered for a 205-d period. Heifers in the HIGH treatment were offered 263 kcal ME/(BWkg)0.75 daily. Heifers in the MEDIUM treatment were offered 238 kcal ME/(BWkg)0.75 daily. Heifers in the LOW-HIGH treatment were offered 157 kcal ME/(BWkg)0.75 daily the first 83 d and 277 kcal ME/(BWkg)0.75 daily for the remainder of the 205 d. Treatments differed in total ME intake (P < 0.001); heifers on the HIGH treatment consumed 3,072+/-59 Mcal/heifer, those on the MEDIUM treatment consumed 2,854+/-21 Mcal/heifer, and those on the LOW-HIGH treatment consumed 2,652+/-19 Mcal/ heifer. At the beginning of breeding, heifers on the HIGH treatment were taller at the hips (P = 0.01) and weighed more (P < 0.001) than heifers in the other two treatments. The percentage of heifers that calved expressed as a fraction of the cows exposed did not differ among treatments (89.7%; P = 0.83). The age of heifer at parturition (P = 0.74) and the time from first bull exposure to calving (P = 0.38) did not differ among treatments. Birth weight of calves (P = 0.80) and the calves' weaning weight (P = 0.60) did not differ among the treatments. Calf survival rate on the LOW-HIGH treatment (73%) was lower than that on the moderate treatment (89%; P = 0.007) but did not differ from that on the HIGH treatment (81%; P = 0.26). The second-calf pregnancy rate (92.8%) for cows with a nursing calf at the start of breeding did not differ between treatments (P = 0.83). These findings suggest that as long as heifers are growing and meet a minimal BW before mating, patterns of growth may be altered in the post-weaning period without a decrease in the ability of the heifer to conceive or a decrease in calf growth potential. However, limit-feeding heifers may decrease first-calf survival. These alterations in postweaning gain through monitoring the amount of feed offered can be used to optimize feed resources.     

Camelina meal and crude glycerin as feed supplements for developing replacement beef heifers

Angus × Gelbvieh rotationally crossbred yearling heifers (n = 99, yr 1; n = 105, yr 2) were used in a 2-yr randomized complete block design experiment with repeated measures to determine the effect of feeding camelina biodiesel coproducts (meal and crude glycerin) on serum concentrations of triiodothyronine, thyroxine, insulin, β-hydroxybutyrate, and glucose, as well as on growth and reproductive performance. Heifers were assigned to 1 of 15 pens, and pens were assigned initially to receive 7.03 k·?heifer(-1)·d(-1) of bromegrass hay plus 0.95 kg·heifer(-1)·d(-1) of 1 of 3 supplements for 60 d before breeding: 1) control (50% ground corn and 50% soybean meal, as-fed basis); 2) mechanically extracted camelina meal; or 3) crude glycerin (50% soybean meal, 33% ground corn, 15% crude glycerin, 2% corn gluten meal; as-fed basis). Preprandial blood samples were collected via the jugular vein on d 0, 30, and 60 of the feeding period. A 2-injection PGF(2α) protocol (d 60 and 70 of the study) was used to synchronize estrus. Heifers were artificially inseminated 12 h after estrus was first detected. Heifers not detected in estrus within 66 h received a GnRH injection and were artificially inseminated. Dietary treatment × sampling period interactions were not detected (P = 0.17 to 0.87). Dietary treatment did not affect BW (P = 0.44 to 0.59) or serum concentrations of thyroxine (P = 0.96), β-hydroxybutyrate (P = 0.46), glucose (P = 0.59), or insulin (P = 0.44). Serum concentrations of triiodothyronine were greater (P = 0.05) in heifers fed camelina meal. Additionally, dietary treatment did not affect the percentage of heifers detected in estrus before timed AI (P = 0.83), first-service pregnancy rates of those heifers detected in estrus (P = 0.97), or overall first-service pregnancy rates (P = 0.58). Heifers fed camelina meal, however, had greater (P = 0.05) first-service pregnancy rates to timed AI than did heifers fed the control and crude glycerin supplements. The cost per pregnancy was similar for heifers fed the crude glycerin or the control supplement, whereas the cost per pregnancy was the least for heifers fed camelina meal. We conclude that camelina coproducts can replace conventional corn-soybean meal supplements in the diets of developing replacement beef heifers.     

Effects of growth-promoting agents and season on yearling feedlot heifer performance

Angus x crossbred heifers (270 per trial) were used in an experiment conducted over one 105-d summer and one 104-d winter feeding period. Treatments were identical for each trial and included: 1) control, 2) estrogenic implant (E), 3) trenbolone acetate implant (TBA), 4) E + TBA (ET), 5) melengestrol acetate (MGA) in the feed, and 6) ET + MGA (ETM). Each treatment was replicated in five pens, with nine heifers per pen in each season. Initial weights (mean = 384 kg, SE = 57) were the same for each season. There were no treatment x season interactions for final BW, ADG, G:F, water intake, or carcass characteristics. Heifers receiving a growth-promoting agent were 11.6 kg (SE = 4.08) heavier and gained 0.108 kg/d (SE = 0.04) more (P < 0.05) than control heifers. Heifers receiving ET gained 0.09 kg/d (SE = 0.032) more (P = 0.05) than heifers not receiving ET. Heifers receiving ET (with and without MGA) had greater G:F (P < 0.05) than control, E, and TBA heifers. Carcass weights of ET-treated heifers were greater (P < 0.05) than carcass weights for unimplanted heifers, those fed MGA only, and heifers receiving either E or TBA implants. Marbling scores were increased (P < 0.05) by feeding MGA to ET-treated heifers. Water intake was greater (P < 0.01) in the summer (31 L/d) than in the winter (18 L/ d), with no difference among implant treatments. Heifers fed in the winter had heavier carcasses, less 12th-rib fat, greater marbling scores, larger LM area, and a greater incidence of liver abscesses than heifers finished in the summer (P < 0.01). A treatment x season interaction (P = 0.07) was evident for DMI during the 35-d coldest and hottest portions of the year. Heifers fed MGA and implanted with ET tended (P = 0.07) to have greater DMI in the summer but lesser DMI in the winter. In general, differences among growth-promotant programs were relatively similar over the entire summer and in winter.     

Time of zeranol implantation on growth, reproduction and calving of beef heifers

Zeranol implants were administered to 250 crossbred heifer calves at 1, 6 or 9 mo of age to evaluate growth, reproduction and calving performance. Heifers were assigned to eight treatment groups with 25 animals per group. Two additional groups of 25 heifers each were used to study the effects of multiple implants at two levels of nutrition on heifer performance. Implants at 1 mo of age (branding) increased heifer weights at 6 mo of age (weaning) by 5 kg (P = .08). Heifers receiving a combination of two implants gained faster (P less than .05) from weaning to breeding (6 to 13 mo) than controls or heifers implanted three times. Implants at either 6 or 9 mo increased (P less than .05) precalving pelvic areas (247 vs 241 cm2 and 248 vs 240 cm2 over controls, respectively). Implants did not affect the percent of heifers reaching puberty prior to breeding season. Conception rates in 62 d of breeding were comparable for implanted and control heifers (93 vs 96%), with the exception of heifers receiving implants at both 1 and 6 mo of age (56%). Calf birth weight, dystocia score, cow rebreeding rate and calf weaning weight were not affected by implant treatments. Heifers that received three implants and were fed at a high nutritional level (gained .62 vs .49 kg/d for regular level after weaning) tended (P greater than .10) to reach puberty at a higher rate prior to breeding and to have a higher total conception rate than implanted heifers on the regular nutrition level.     

Incidence of puberty in beef heifers fed high- or low-starch diets for different periods before breeding

Spring-born Hereford x Angus heifers (n = 206) were used to determine effects of energy supplementation programs and amount of starch in the diet on incidence of puberty. In Exp. 1, heifers (205 +/- 5 kg; n = 68) grazing dormant native pasture were fed 0.9 kg/d (as-fed basis) of a 42% CP supplement from November until February 14. Heifers were stratified by weaning weight and allotted randomly to treatment before breeding (May to July). Treatments were 1) 0.9 kg (as-fed basis) of a 42% CP supplement/d and pasture (control); 2) a high-starch (HS) diet (73% corn; 53% starch) fed in a drylot for 60 d (HS-60); 3) a HS diet fed in drylot for 30 d (HS-30); or 4) a low-starch (LS) diet (49% corn; 37% starch) self-fed on pasture for 30 d (LS-30). The HS-60 and HS-30 heifers were limited-fed to gain 0.9 kg/d, and the LS-30 heifers had ad libitum access to the diet. High-starch-60 and LS-30 heifers were heavier (P < 0.05) than control and HS-30 heifers at the beginning of the breeding season. Thirty-one, 25, and 26% more HS-60 heifers were pubertal (P < 0.05) on May 1 compared with LS-30, HS-30, and control heifers, respectively. At puberty, HS-60 heifers were 24 and 22 d younger (P < 0.05) than LS-30 and control heifers, and 31 kg lighter (P < 0.01) than LS-30 heifers. In Exp. 2, heifers grazed dormant pasture and were fed 0.9 kg (as-fed basis) of a 42% CP supplement/d from weaning in October to late February; then heifers were assigned randomly to treatments for 60 d before the breeding season. In two years, control heifers (n = 46) grazed pasture and received 0.9 kg of SBM supplement/d; LS (n = 46) heifers were self-fed a distiller's grain and soybean hull-based diet in drylot; and HS heifers (n = 46) were limited-fed a corn-based diet in drylot. During treatment, HS and LS heifers had greater weight gains than control heifers. Pubertal BW (313 +/- 6 kg) was not influenced by treatment, but HS and LS heifers were younger (P < 0.03) than control heifers at puberty. During a 60-d breeding period, the incidence of puberty was greater (P < 0.05) for HS and LS heifers than for control heifers and was greater (P < 0.05) in HS than in LS heifers in Year 1. Feeding a LS or a HS diet for 30 d before breeding may be inadequate to stimulate puberty in beef heifers, but feeding a diet with a greater amount of starch for 60 d before breeding may increase the incidence of puberty during breeding of heifers that have inadequate yearling weight.     

Reproductive response of yearling beef heifers to a melengestrol acetate-prostaglandin F2 alpha estrus synchronization system.

A study was designed to evaluate estrus response and fertility after treatment with melengestrol acetate (MGA) and prostaglandin F2 alpha (PGF2 alpha) in yearling beef heifers. Three hundred four heifers at three locations were allotted to one of two treatments: Treatment 1 served as a nonsynchronized control (CON); and heifers in Treatment 2 received .5 mg of MGA.animal-1.d-1 for 14 d and 25 mg of prostaglandin F2 alpha (PGF2 alpha) 17 d after MGA (MGA-PGF). Heifers in CON and MGA-PGF groups were artificially inseminated 12 h after observed estrus for 21 and 6 d after PGF2 alpha, respectively. Blood samples were collected from each heifer 10 d before and on the day MGA feeding began and 10 d before and on the day PGF2 alpha was administered. Heifers with concentrations of serum progesterone greater than 1 ng/mL on either date before administration of MGA or PGF2 alpha were considered pubertal. More (P = .02) prepubertal heifers that received MGA attained puberty by initiation of breeding than did CON heifers (72 vs 45%, respectively). The proportion of heifers that displayed estrus within 6 d after PGF2 alpha was greater (P less than .001) for MGA-PGF than for CON heifers (77 vs 25%, respectively) but was also influenced by location (P = .03). Conception rate at first service for MGA-PGF heifers that attained puberty during MGA feeding and before PGF2 alpha was not different (P = .50) from that of CON that attained puberty during the same period.(ABSTRACT TRUNCATED AT 250 WORDS)     

Evaluation of Three Estrous Synchronization Protocols in Beef Heifers

Objectives of this study were to evaluate synchronization, conception, and pregnancy rates of heifers synchronized with melengestrol acetate (MGA)-prostaglandin F_2α (PGF_2α,), Select Synch, or Select Synch preceded by MGA (MGA-Select Synch). Heifers in the MGA-PGF_2α group (n = 209; BW = 378 kg) received MGA (0.5 mg/ d per heifer) for 14 d and PGF_2α (25 mg) 19 d later. Select Synch heifers (n = 213; BW = 374 kg) received gonadotropin-releasing hormone (GnRH; 100 μg) followed by PGF_2α (25 mg) 7 d later. The MGA-Select Synch heifers (n = 210; BW = 373 kg) were fed MGA (0.5 mg/d per heifer) for 7 d, GnRH (100 μg) the day following the last MGA feeding, and PGF_2α (25 mg) 7 d after GnRH. More (P<0.01) heifers were in estrus 1 to 4 d before PGF2a administration in both the Select Synch (20%) and MGA-Select Synch (24%) groups than in the MGA-PGF_2α (4%) group. Pregnancy rates for heifers in estrus early (d 1 to 4 before PGF_2α) were greater (P<0.05) for both Select Synch (55%) and MGA-Select Synch (63%) compared with MGA-PGF_2α heifers (18%). Synchronization rate (detected after PGF_2α) was greater (P<0.01) for MGA-PGF_2α heifers (86%) compared with Select Synch (66%) and MGA-Select Synch (68%) heifers; however, conception rate did not differ (P=0.13) and averaged 72, 63, and 62% for MGA-PGF_2α, Select Synch, and MGA-Select Synch heifers, respectively. Select Synch (52%), MGA-Select Synch (58%), and MGA-PGF_2α protocols (61%) provided similar (P=0.18) overall AI pregnancy rates; however, more heifers were in estrus before PGF_2α administration in protocols using GnRH.     

Effects of melengestrol acetate on the inflammatory response in heifers challenged with Mannheimia haemolytica

Previous research from our laboratory has indicated that melengestrol acetate (MGA) added to the diet during the first 35 d after arrival in the feedlot improves growth rates and tends to reduce chronic respiratory disease in heifers naturally challenged with bovine respiratory disease. The current study was conducted to provide further insight into the possible immunomodulatory effects of MGA. Crossbred heifers (n = 48; 232 +/- 5.5 kg of BW) were used in a randomized complete block design to determine the effects of MGA on lung pathology and markers of inflammation after Mannheimia haemolytica challenge. On d 0, cattle were blocked by BW and randomly assigned, within block, to diets (54% concentrate) that provided 0 or 0.5 mg of MGA per heifer daily for the duration of the experiment. Inoculum containing from 1.3 x 10(9) to 1.7 x 10(9) cfu of M. haemolytica (20 mL) was instilled at the bifurcation of the trachea on d 14. Blood samples were collected, clinical observations were made, and rectal temperatures were recorded for each animal at 0, 12, 24, 48, 72, 96, 120, and 138 h after inoculation. Heifers fed MGA had greater circulating concentrations of eosinophils and postchallenge concentrations of segmented neutrophils and white blood cells (P < 0.01) than controls, as well as elevated plasma protein, serum haptoglobin, and fibrinogen after M. haemolytica challenge (P < 0.01). Heifers fed MGA had lower plasma glucose (P < 0.01), greater plasma urea N (P = 0.02), and elevated respiratory indices (P < 0.01) compared with controls. Necropsies performed on d 6 after inoculation suggested that M. haemolytica challenge was relatively mild, because lesions were confined to a small portion of the lungs. On a 0 to 100 scale, average lung lesion scores were 3 and 1 for MGA-fed and control groups, respectively (P < 0.06). Heifers fed MGA before mild M. haemolytica challenge were more susceptible to infection, as evidenced by a greater number of heifers fed MGA exhibiting pulmonary lesions 138 h after inoculation than controls (14 out of 23 vs. 6 out of 24 for MGA and controls, respectively; P < 0.02).     

Effect of early weaning on the performance of three-year-old,first-calf beef heifers and calves reared in the subtropics

The objective of this study was to investigate the effectiveness of early weaning fall-born calves on heifer and calf performance in Florida. Over two consecutive years, 3-yr-old Braford and Brahman x Angus first-calf heifers were assigned randomly to one of two treatments; early-weaned (EW, n = 20 and 30 for yr 1 and 2, respectively) and normal-weaned (NW, n = 20 and 38 for yr 1 and 2, respectively). Calves were EW on January 23 and 3 for yr 1 and 2, respectively. Following EW, all first-calf heifers were returned to bahiagrass (Paspalum notatum) pastures with the mature cowherd. Early-weaned calves were maintained on annual ryegrass (Lolium multiflorum) pastures at 8.2 and 10.7 calves/ha for yr 1 and 2, respectively, and were provided supplemental grain mixture (14% CP) at 1.0% of BW daily. Normal-weaned calves remained with their dams in the mature cowherd on bahiagrass. Final calf BW was collected on April 17 (d 84) and April 24 (d 111) for yr 1 and 2, respectively. Early-weaned calves had a greater (P < 0.001) ADG (0.17 kg/d) in yr 1, but a lower (P < 0.001) ADG (-0.24 kg/d) in yr 2 compared with NW calves. Early weaning resulted in heavier first-calf heifers with greater BCS at the time of normal weaning (August 1; 491 vs. 452 kg, with BCS = 6.34 vs. 4.75 for EW and NW heifers, respectively; SEM = 5.0 and 0.07). Heifers with EW calves had a higher (P < 0.07) pregnancy rate during both years than normal-weaned heifers (89.5 vs. 50.0 and 96.7 vs. 80.0% pregnant during yr 1 and 2, respectively). Early-weaned, first-calf heifers also had a lower (P < 0.05) calving interval in yr 2 (384 vs. 404 d; SEM = 6.0). These data suggest that EW will improve body condition of first-calf heifers resulting in an increased pregnancy rate. Early-weaned calves maintained on winter ryegrass provide producers with the ability to optimize early-weaned calf performance, while capitalizing on low cost of gain and favorable spring markets.     

Comparison of long-term progestin-based protocols to synchronize estrus before fixed-time artificial insemination in beef heifers

Two experiments were conducted to compare pregnancy rates resulting from fixed-time AI (FTAI) after administration of 1 of 2 long-term controlled internal drug release (CIDR)-based protocols. Heifers were assigned to treatment by age, BW, and pubertal status. The CIDR Select-treated heifers (Exp. 1, n = 37; Exp. 2, n = 192) received a CIDR (1.38 g of progesterone) from d 0 to 14, followed by 100 μg of GnRH, intramuscularly (i.m.) 9 d after CIDR removal (d 23) and PGF(2α) (25 mg, i.m.) 7 d after GnRH treatment (d 30). Heifers assigned to the Show-Me-Synch protocol (Exp. 1, n = 40; Exp. 2, n = 200) received a CIDR from d 0 to 14, followed by PGF(2α) 16 d later (d 30). Artificial insemination was performed at 72 or 66 h after PGF(2α) treatment for the CIDR Select- and Show-Me-Synch-treated heifers, respectively, and each heifer was given GnRH (100 μg, i.m.) at the time of AI. In Exp. 1, ovaries of each heifer were examined by transrectal ultrasonography on d 23 and 30 to characterize follicular dynamics. Follicles ≥5 mm and the presence of corpora lutea were recorded. On d 25, ovaries of each heifer were examined to characterize the status of dominant follicles recorded on d 23. Heifers were fitted with HeatWatch (DDx Inc., Denver, CO) estrus-detection transmitters at PGF(2α) to characterize estrus distribution up to FTAI. The diameter of dominant follicles on d 23 at PGF(2α) and on d 30, and the estrous response after PGF(2α) treatment up to the point of FTAI did not differ between CIDR Select- and Show-Me-Synch-treated heifers. Concentrations of progesterone in serum at PGF(2α) were greater (P = 0.07) in Show-Me-Synch- than CIDR Select-treated heifers (6.0 vs. 4.8 ng/mL, respectively). Pregnancy rates of heifers resulting from FTAI did not differ (P = 0.33) between CIDR Select- and Show-Me-Synch-treated heifers (CIDR Select, 59%; Show-Me-Synch, 70%). In Exp. 2, FTAI pregnancy rates tended (P = 0.07) to be greater in Show-Me-Synch-treated (62%) than in CIDR Select-treated (51%) heifers. Pregnancy rates at the end of the breeding season did not differ (P = 0.72; CIDR Select, 85%; Show-Me-Synch, 83%) between treatments. In summary, pregnancy rates resulting from FTAI were comparable for heifers assigned to each of the 2 long-term progestin-based protocols. The reduced treatment cost and animal handling associated with administration of the Show-Me-Synch protocol offer distinct advantages over the CIDR Select protocol despite similarities in pregnancy rates resulting from FTAI.     

Pregnancy rates of beef heifers bred either on puberal or third estrus

The objective of this study was to determine if pregnancy rates (PR) differed between beef heifers bred to fertile bulls on either their puberal (E1, n = 89) or third (E3, n = 67) estrus. Heifers were obtained from two lactations (Manhattan, L1; and Miles City, L2), and the experiment was conducted at Miles City. Heifers were assigned randomly within location to either E1 or E3. Heifers were fed to gain .56 kg.head-1 X d-1 and observed twice daily for estrus. After exhibiting first estrus (puberty) and breeding, each heifer in E1 was palpated rectally on d 6, 9 and 12 +/- 1 d (estrus = d 0) for the presence of a corpus luteum, and a venous blood sample was collected for assay of progesterone by radioimmunoassay. Heifers in E3 were palpated and bled on the same schedule as heifers in E1 after first estrus and after being bred to a fertile bull at third estrus. Pregnancy rates were determined by rectal palpation at approximately 38 d post-breeding. Location of origin did not affect (P greater than .10) weight at puberty or weight at breeding; however, heifers from L1 were younger (P less than .05) than heifers from L2 at puberty and breeding. Pregnancy rates were 57 and 78% for heifers in E1 and E3, respectively (P less than .05). Weight at breeding did not influence (P greater than .10) pregnancy rates. The probability of heifers in E1 becoming pregnant increased (P less than .05) with increasing age, while age was not a factor (P greater than .10) for heifers in E3. These results indicated that fertility of puberal estrus in beef heifers is lower than third estrus. Higher fertility of third estrus may be related to maturational changes associated with cycling activity.     

Postweaning growth and reproduction of beef heifers exposed to calve at 24 or 30 months of age in spring and fall seasons.

Angus and Angus x Hereford heifer calves born in spring or fall seasons were allotted by weight at weaning to be exposed to bulls for calving at either 24 or 30 mo of age. Comparisons were made 1) within birth season--heifers born in the same season were first exposed to bulls at either 14 to 16 or 19 to 22 mo of age and 2) within breeding season--heifers born in different but consecutive seasons (spring-fall or fall-spring) were exposed to bulls during the same season but at different ages. Spring-born heifers exposed for 30-mo calving were heavier (336 vs 302 kg) and taller (P less than .01) at breeding than those exposed for 24-mo calving. Also, more were observed in estrus and became pregnant (P less than .01) during a 21-d AI breeding period, but season-long (63 d) pregnancy rates were not different (86%). Fall-born heifers exposed for 24- or 30-mo calving were similar for weight, hip height, and body condition score (BCS) at breeding, but season-long pregnancy rates were 71 and 94%, respectively (P less than .01). Although fall-born heifers were heavier at weaning (P less than .01), spring-born heifers were heavier, taller, and had higher BCS at breeding (P less than .01), regardless of age. Within spring breeding seasons, breeding weight and initial reproduction were not affected by heifer age. Within fall breeding seasons, older heifers were larger (P less than .01) and had greater reproductive performance (P less than .05) than younger heifers. Age at breeding, irrespective of season, consistently affected (P less than .01) both hip height (116 vs 113 cm) and pelvic area (209 vs 178 cm2) for older vs younger heifers, respectively. These data indicate that heifers exposed for 30-mo first calving are heavier, taller, have larger pelvic area, and have greater reproductive performance than those exposed for 24-mo first calving.     

Supplementation with Whole Sunflower Seeds Before Artificial Insemination in Beef Heifers

The objective of this study was to evaluate synchronization and pregnancy rates of beef heifers supplemented with 0.91 kg of whole sunflower seeds for 0, 30, or 60 d before AI. Beef heifers from four locations (n = 1,014) were assigned by BW to treatment (within location) and randomly to AI sire. Heifers at Location 1 (n = 176; mean BW = 332 kg) received either 0- or 60-d sunflower seed treatments. Heifers at Location 2 (n = 397; mean BW = 334 kg) were fed sunflower seeds for 0, 30, or 60 d. Heifers at Locations 3 (n = 211; mean BW = 345 kg) and 4 (n = 230; mean BW = 343 kg) received 0- or 30-d sunflower seed treatments. Within location, diets were formulated to be isocaloric and isonitrogenous. All heifers received melengesterol acetate (0.5 mg/d per head) for 14 d followed 19 d later by an injection of prostaglandin F_2a (PGF) (25 mg). Heifers were bred by AI according to the AM/PM rule except on d 3 when all heifers that had not exhibited estrus were artificially inseminated in mass. Neither 72-h estrous response nor pregnancy rate was affected (P>0.10) by 30- or 60-d sunflower feeding. In summary, feeding 0.91 kg of whole sunflower seeds for either 30 or 60 d before AI did not improve estrous response or pregnancy rate when compared with controls.     

Supplementation to meet metabolizable protein requirements of primiparous beef heifers: II. Pregnancy and economics

Metabolizable protein (MP) requirements of spring calving heifers increase over the winter due to advancing gestation. The MP content of grazed winter forage is low, which may result in an MP deficiency. The objective was to compare the response of supplementing pregnant yearling heifers to meet MP requirements versus conventional CP supplementation. In 1997-98 and in 1998-99, pregnant, March-calving heifers (2,120 animals; 358 kg) at two locations of a commercial ranch in the Nebraska Sandhills, were used following breeding through calving as 2-yr-olds (cows). Heifers were randomly allotted to one of two supplementation treatments (about 265 heifers/treatment) each year at each of two locations (Ashby and Whitman, NE). Treatments were 1) supplementation to meet MP requirements (MPR) or 2) supplementation to meet CP requirements (CPR). Heifers grazed upland range and meadow and were offered supplements three times weekly from mid-September to mid- or late-February. Increasing amounts of meadow hay were fed from mid-December through calving. After supplementation ended in February, heifers were managed in one group at each location until the following October. Body weights and body condition scores were taken in September, February, and October. Two-yr-old pregnancy rates were determined via rectal palpation in October. Capital budgeting techniques were used to determine the economic return of supplementation strategies. There were no differences in BW (P = 0.41) or body condition score (P = 0.99) change during the winter among treatment groups across years and locations, but MPR cows were heavier (425 kg) than CPR cows (421 kg) at the time of 2-yr-old pregnancy testing (P = 0.07). Pregnancy rate was higher (P = 0.001) in the MPR (91%) compared to the CPR treatment (86%). Regression analysis showed that the response of the MPR treatment on pregancy rates tended to be negatively correlated with precalving body condition score (P = 0.11), body condition score loss over the winter (P = 0.07), and body condition score at weaning of the first calf (P = 0.08). The improvement in 2-yr-old pregnancy by supplementing to meet MP requirements improved the value of each bred heifer by 13.64 dollars. We conclude that balancing MP requirements during gestation may result in a subsequent increase in 2-yr-old pregnancy and increase the value of young females.