Detection of Soil Organic Nitrogen in Xylem Sap Collected from Nonmycorrhizal Plants using an Immunological Technique

Phosphate buffer–extractable organic nitrogen (PEON) is considered to be a ubiquitous high-molecular-weightnitrogen (N) compound in soil and may be an important N source for some plant species. We examined whether nonmycorrhizal plants qing-geng-cai (Brassica rapa var. chinensis) and spinach (Spinacia oleracea L. cv. Atras) could uptake PEON from the soilusing animmunoassay that was previously developed from a PEON determination method using anti-PEON IgG. Xylem sap analysis of spinach using an enzyme-linked immune sorbent assay (ELISA) showed that this plant hadhigh capabilities to take up PEON from the soil. Spinach and qing-geng-cai exuded substantial amounts of oxalate or citrate under inorganic N-deficient conditions, indicating exudation of organic acids is probably involved in PEON mobilization. Further results of immunohistochemical localization of PEON in the root section of qing-geng-cai revealed that intact or at least a fraction of PEON absorbed by the epidermis passes through the cortex cell and intercellular space. Our findings indicated that some specific plants have the ability to mobilize PEON by root exudation and take up mineralizable organic N by endocytic invagination.     

Organic nitrogen stimulates the heterotrophic nitrification rate in an acidic forest soil

Many previous studies have demonstrated that heterotrophic nitrification processes play an important role in the production of NO₃⁻ in acidic soils. However, it is not clear whether a low concentration of nitrogenous organic compounds support heterotrophic nitrification processes in natural soils. In this study, we performed an ¹⁵N tracer experiment with a glycine concentration gradient (20, 40, 80, and 160 mg N kg⁻¹) to investigate the effect of the organic nitrogen concentration on the heterotrophic nitrification rate and its relative contribution to the total nitrification of the studied acidic forest soil. This experiment demonstrated that ¹⁵N–NO₃⁻ accumulated over time with all nitrogen treatments in the presence of acetylene, confirming that heterotrophic nitrification occurred even at a low organic nitrogen concentration (20 mg kg⁻¹) in the studied acidic forest soil. In the presence of acetylene, the ¹⁵N–NO₃⁻ concentration in the 20 and 40 mg kg⁻¹ glycine-N treatments was significantly lower than in the 80 and 160 mg kg⁻¹ glycine-N treatments (p < 0.05), indicating that a high organic nitrogen concentration stimulated the heterotrophic nitrification rate. There was no significant difference in the average contribution of heterotrophic nitrification to total nitrification among the different nitrogen treatments, suggesting that the organic nitrogen concentration did not affect the relative contribution of heterotrophic nitrification to total nitrification in the studied acidic soil. Our results confirmed that a low concentration of organic N (20 mg kg⁻¹) supported heterotrophic nitrification in the studied soil. The organic nitrogen concentration stimulates the heterotrophic nitrification rate, but does not affect the relative contribution of heterotrophic nitrification to total nitrification in the studied acidic soil.     

Phosphate buffer–extractable organic nitrogen as an index of soil‐N availability for sorghum and pearl millet

The availability of soil nitrogen (N) is usually quantified by the amount of mineralized N as determined after several weeks of soil incubation. Various alternative methods using chemical solvents have been developed to extract the available organic N, which is easily mineralized. We compared one such solution, neutral phosphate buffer (NPB), with conventional incubation and 0.01 M–CaCl₂ extraction, as measures of soil N available to two major cereal crops of the semiarid tropics, based on the total N uptake by plants in a pot experiment. Mineralized N had the highest correlation with N uptake by pearl millet (Pennisetum glaucum L., r = 0.979***) and sorghum (Sorghum bicolor [L.] Moench, r = 0.978***). NPB‐extractable N was also highly correlated with N uptake (pearl millet, r = 0.876***; sorghum, r = 0.872***). Only one major peak was detected when NPB extracts were analyzed using size‐exclusion high‐performance liquid chromatography, regardless of soil properties. In addition, the organic N extracted with NPB was characterized by determining the content of peptidoglycan, the main component of bacterial cell walls. Although the characteristics of NPB‐extractable organic N are still unclear, it offers a promising quick assay of available N.     

萝卜适宜施氮量和氮肥基追比例研究

运用15N示踪法研究了大田条件下不同氮肥用量与施肥方式对萝卜氮素吸收、分配及肉质根产量的影响。试验设置3个氮水平（0、 60和120 kg/hm2）和两种基追肥比例［基肥∶破肚期肥料∶膨大期肥料=50%∶20%∶30%（A）和30%∶20%∶50%（B）］,共5个处理,依次记作 N0、N60A、N60B、N120A、N120B。结果表明,在施N 0120 kg/hm2范围内,随氮施用量的增加,萝卜吸收的肥料氮素、土壤氮素数量及肥料氮在土壤中的残留量显著增加,氮素的吸收利用率和土壤残留率显著下降,氮素损失率显著增加。当氮用量为120 kg/hm2 时, N120A和N120B处理萝卜吸收的肥料氮素、土壤氮素及肥料氮在土壤中的残留量分别为30.50、 53.64、 14.88 kg/hm2和35.56、 56.61、 17.81 kg/hm2,采收期肉质根产量分别为67.6 t/hm2和72.5 t/hm2,比对应的低氮处理（N60A和N60B）分别增加64.07%和66.67%,且N120B处理萝卜氮素吸收利用率显著提高。因此,适量施氮并增加肉质根膨大期的施氮比例,可有效提高氮肥利用率,显著增加萝卜肉质根产量。在本试验条件下,施氮量为120 kg/hm2, 按照基肥∶破肚期肥料∶膨大期肥料比例30%∶20%∶50%进行施肥,是兼顾产量和氮肥利用效率的最佳氮肥运筹方式。     

Soluble organic nitrogen in temperate forest soils

Very few studies have been related to soluble organic nitrogen (SON) in forest soils. However, this nitrogen pool could be a sensitive indicator to evaluate the soil nitrogen status. The current study was conducted in temperate forests of Thuringia, Germany, where soils had SON (extracted in 0.5 M K₂SO₄) varying from 0.3 to 2.2% of total N, which was about one-third of the soil microbial biomass N by CFE. SON in study soils were positively correlated to microbial biomass N and soil total N. Multiple regression analysis also showed that mineral N negatively affected SON pool. The dynamics of the SON was significantly affected by mineralization and immobilization. During the 2 months of aerobic incubation, the SON were significantly correlated with net N mineralization and microbial biomass N. SON extracted by two different salt solution (i.e. 1 M KCl and 0.5 M K₂SO₄₎ were highly correlated. In mineral soil, SON concentrations extracted by 1 M KCl and 0.5 M K₂SO₄ solutions were similar. In contrast, in organic soil layer the amount of KCl-extractable SON was about 1.2-1.4 times higher than the K₂SO₄-extractable SON. Further studies such as the differences of organic N form and pool size between SON and dissolved organic N (DON) are recommended.     

新疆石河子地区玉米产量及氮素平衡的施氮量阈值研究

【目的】合理施用氮肥不仅会提高肥料利用率,还会降低氮素面源污染的风险。通过2年田间肥料定位试验,研究北疆灰漠土区不同氮肥用量下,土壤无机氮积累量、 氮素平衡和玉米产量间的相互关系,为氮肥合理施用提供依据。【方法】研究采用肥料田间定位试验,小区试验于2011-2012年开展,设计6个氮肥（N）用量水平: 0、 225、 300、 375、 450、 600 kg/hm2,分别以N0、 N225、 N300、 N375、 N450、 N600表示,其中300 kg/hm2为当地玉米农田氮肥推荐用量,磷肥（P2O5）施用量为75 kg/hm2,钾肥（K2O）施用量为37.5 kg/hm2。【结果】 1）施用氮肥增加了土壤硝态氮和铵态氮残留量,硝态氮主要残留于060 cm土层,铵态氮主要分布在020 cm土层深度。2011年试验中,土壤无机氮残留量随氮肥用量增加而显著增加,与对照相比,施氮处理无机氮残留量增幅为12%~102%,与施氮量呈指数增长关系。2012年氮肥用量对土壤无机氮残留量的影响与2011年相似。2）施氮量 225 kg/hm2时,0100 cm土层深度土壤无机氮积累量降低,表现为负积累效应,N0和N225处理下2012年土壤无机氮积累量分别较2011年降低165%和170%; 施氮量高于 300 kg/hm2时,土壤无机氮积累量显著增加,表现为富集现象,其中,N375、 N450和N600处理下2012年土壤无机氮积累量分别较2011年增加17%、 388%、 170%。土壤无机氮积累量与施氮量显著呈二次抛物线关系,2011年回归方程为y=0.0001x2 + 0.1013x－22.537（R2 = 0.9288）,无机氮无积累时施氮量为187 kg/hm2; 2012年为 y = 0.0003x2 + 0.1417x - 52.78（R2 = 0.9583）,无机氮无积累时施氮量为245 kg/hm2。土壤氮素表观损失量和氮素盈余量的增加幅度随氮肥用量增加而显著加大。3）氮肥投入可提高玉米产量,产量与施氮量呈显著的二次抛物线或线性加平台的关系,施氮量高于300 kg/hm2时,玉米产量与最高产量差异不显著; 产量与无机氮积累量呈二次抛物线形关系,当土壤无机氮达到平衡时,玉米产量显著低于最高产量,当玉米产量达到最大时,土壤无机氮有一定积累。氮肥利用率则随氮肥用量增加呈指数关系显著降低。施氮量270 kg/hm2为产量与氮肥利用率的交点,施氮量340 kg/hm2 是土壤无机氮残留量与氮肥利用率的交点。【结论】利用产量效应、 环境效应与肥料效应函数的交点确定氮肥投入阈值,是较为优化的方法。合理的氮肥投入不仅能获得玉米高产,降低氮素面源污染风险,还能获得较高的氮肥利用率。因此,施氮量260340 kg/hm2为本研究区玉米高产与环境友好的氮肥投入阈值。     

水氮耦合对绿洲灌区土壤硝态氮运移及甜瓜氮素吸收的影响

在地处沙漠绿洲的甜瓜种植区,研究不同水、 氮输入量对土壤氮素平衡和运移的影响,为当地甜瓜生产的水肥管理提供科学依据。通过2009、 2010连续两年田间裂区试验,研究了不同灌水量（1500、 2100、 2700、 3300 m3/hm2,以W1500、 W2100、 W2700和W3300表示）和施氮量（N 0、 120、 240、 360 kg/hm2,以N0、 N120、 N240和N360表示）对土壤硝态氮分布、 累积和甜瓜的水、 氮吸收以及产量的影响。结果表明,甜瓜收获后各处理土壤硝态氮含量在040 cm土层最高, 0200 cm土层呈现先减少后增加再减少的变化趋势,且施氮量越大,硝态氮在80120 cm土层大量累积的趋势越明显。土壤硝态氮累积量随施氮量的增加而增加,随灌水量的增加而减少,灌水量超过2700 m3/hm2 时,仅有不到53%的硝态氮留存在0100 cm土层。甜瓜产量和果实氮素吸收量随灌水量和施氮量的增加而提高,但在W3300N360处理略有下降。氮素回收率随施氮量的增加持续降低,氮收获指数以处理W2700N240最大,水分利用效率以W1500N240处理最大。W2700N240处理能够兼顾甜瓜产量,平衡氮素吸收运移与土壤中硝态氮的留存空间3个方面,是绿洲灌区甜瓜种植的高产高效的水氮输入模式。     

NO3‐N Soil Test—approaches to use and interpretation

Abstract

Nitrate‐nitrogen (NO₃‐N) soil tests have been instituted by several Great Plains States to aid in making nitrogen fertilizer recommendations. The NO₃‐N soil test is useful in the Great Plains because climatic conditions are such that NO₃‐N leaching is low and soil rooting depths are great.

Essentially four basic approaches to the use and interpretation of the NO₃‐N soil test have been developed and are discussed. They are: (1) total amount of NO₃‐N extracted from a two foot soil profile is available to the growing crop; (2) only a portion of the extractable NO₃‐N is available to the growing crop; (3) other mineral nitrogen, such as NH₄‐N, is just as available to the growing crop and (4) some nitrogen becomes available from the soil organic matter. The NO₃‐N soil test requires that deep samples (two feet or more) be taken and that they are air‐dried as rapidly as possible. Late fall or early spring soil samples are necessary for interpretation of the NO₃‐N soil test.     

黄土高原人工油松林土壤碳氮对短期氮添加的响应

为了更好地理解土壤碳氮元素对氮添加的响应,通过短期原位模拟氮沉降试验,揭示黄土高原子午岭人工油松(Pinus tabulaeformis Carrière)林土壤碳氮对外源氮添加的响应过程和机制。从2015—2016年设置4个氮添加水平,分别为对照(0kg/(hm~2·a),N0)、低氮(50kg/(hm~2·a),N50)、中氮(100kg/(hm~2·a),N100)和高氮(200kg/(hm~2·a),N200),研究人工油松林地不同深度土层土壤有机碳和全氮以及土壤碳氮储量对模拟氮添加的响应。结果表明:土层对土壤有机碳、全氮和碳氮储量有显著影响,上层土壤有机碳、全氮和碳氮储量显著高于下层土壤;氮添加水平对土壤有机碳、土壤碳储量影响不显著,但可显著影响土壤全氮和氮储量。此外,土壤有机碳、全氮和碳氮储量和土壤碳氮储量比受地下生物量碳氮比的影响显著。因此,短期氮添加对人工油松林地土壤碳的影响不显著,但可显著影响土壤氮,地下生物量碳氮比是影响土壤碳氮的重要因素。     

Elevated CO2, but not defoliation, enhances N cycling and increases short-term soil N immobilization regardless of N addition in a semiarid grassland

Elevated CO₂ and defoliation effects on nitrogen (N) cycling in rangeland soils remain poorly understood. Here we tested whether effects of elevated CO₂ (720 μl L⁻¹) and defoliation (clipping to 2.5 cm height) on N cycling depended on soil N availability (addition of 1 vs. 11 g N m⁻²) in intact mesocosms extracted from a semiarid grassland. Mesocosms were kept inside growth chambers for one growing season, and the experiment was repeated the next year. We added ¹⁵N (1 g m⁻²) to all mesocosms at the start of the growing season. We measured total N and ¹⁵N in plant, soil inorganic, microbial and soil organic pools at different times of the growing season. We combined the plant, soil inorganic, and microbial N pools into one pool (PIM-N pool) to separate biotic + inorganic from abiotic N residing in soil organic matter (SOM). With the ¹⁵N measurements we were then able to calculate transfer rates of N from the active PIM-N pool into SOM (soil N immobilization) and vice versa (soil N mobilization) throughout the growing season. We observed significant interactive effects of elevated CO₂ with N addition and defoliation with N addition on soil N mobilization and immobilization. However, no interactive effects were observed for net transfer rates. Net N transfer from the PIM-N pool into SOM increased under elevated CO₂, but was unaffected by defoliation. Elevated CO₂ and defoliation effects on the net transfer of N into SOM may not depend on soil N availability in semiarid grasslands, but may depend on the balance of root litter production affecting soil N immobilization and root exudation affecting soil N mobilization. We observed no interactive effects of elevated CO₂ with defoliation. We conclude that elevated CO₂, but not defoliation, may limit plant productivity in the long-term through increased soil N immobilization.     

Sequential extraction methods for soil organic nitrogen

(pp. 833–841)

In order to learn the status of available soil nitrogen (N), two sequential extraction methods (A and B) were tested. Proposed methods are as follows.

(A) Extraction with water, KCl, acetic acid, 1/15 M phosphate buffer, sulfuric acid, and NaOH.

(B) Extraction with water, KCl, 0.01 M sulfuric acid, 0.1 M sulfuric, 0.2 M sulfuric acid and 0.4 M sulfuric acid.

1) NO₃-N was only detected in a solution extracted with water. Major N in an extract with KCl was in ammonium form. Extracts with phosphate buffer and sulfuric acid contained organic-N only. When these two fractions were examined by size exclusion chromatography (HPLC-SEC), only one major peak in each extract appeared.

2) Minerals, such as Al and Fe in each extract were determined using ICP (Varian Co., Ltd.). With the increase in concentration of sulfuric acid, the high Fe concentration in each extract increased. On the other hand, much higher Al was found in extracts with lower concentrations of sulfuric acid compared to Fe.

3) Though some crop species such as spinach and carrot show the potential to take up organic-N in a soil applied with organic matter, this sequential soil-nitrogen extraction method may be useful to evaluate availability of soil nitrogen fertility with more accuracy compared to conventional methods in the case of these superior crop species.     

Microbial mineralization of biochar and wheat straw mixture in soil: A short-term study

A short-term incubation study was carried out to investigate the effect of biochar addition to soil on CO₂ emissions, microbial biomass, soil soluble carbon (C) nitrogen (N) and nitrate–nitrogen (NO₃–N). Four soil treatments were investigated: soil only (control); soil + 5% biochar; soil + 0.5% wheat straw; soil + 5% biochar + 0.5% wheat straw. The biochar used was obtained from hardwood by pyrolysis at 500 °C. Periodic measurements of soil respiration, microbial biomass, soluble organic C, N and NO₃–N were performed throughout the experiment (84 days). Only 2.8% of the added biochar C was respired, whereas 56% of the added wheat straw C was decomposed. Total net CO₂ emitted by soil respiration suggested that wheat straw had no priming effect on biochar C decomposition. Moreover, wheat straw significantly increased microbial C and N and at the same time decreased soluble organic N. On the other hand, biochar did not influence microbial biomass nor soluble organic N. Thus it is possible to conclude that biochar was a very stable C source and could be an efficient, long-term strategy to sequester C in soils. Moreover, the addition of crop residues together with biochar could actively reduce the soil N leaching potential by means of N immobilization.     

The effect of precipitation and application rate on dicyandiamide persistence and efficiency in two Irish grassland soils

The nitrification inhibitor dicyandiamide (DCD) has had variable success in reducing nitrate () leaching and nitrous oxide (N₂O) emissions from soils receiving nitrogen (N) fertilizers. Factors such as soil type, temperature and moisture have been linked to the variable efficacy of DCD. As DCD is water soluble, it can be leached from the rooting zone where it is intended to inhibit nitrification. Intact soil columns (15 cm diameter by 35 cm long) were taken from luvic gleysol and haplic cambisol grassland sites and placed in growth chambers. DCD was applied at 15 or 30 kg DCD/ha, with high or low precipitation. Leaching of DCD, mineral N and the residual soil DCD concentrations were determined over 8 weeks high precipitation increased DCD in leachate and decreased recovery in soil. A soil × DCD rate interaction was detected for the DCD unaccounted (proxy for degraded DCD). In the cambisol, degradation of DCD was high (circa 81%) and unaffected by DCD rate. In contrast, DCD degradation in the gleysol was lower and differentially affected by rate, 67 and 46% for the 15 and 30 kg/ha treatments, respectively. Variation in DCD degradation rates between soils may be related to differences in organic matter content and associated microbiological activity. Variable degradation rates of DCD in soil, unrelated to temperature or moisture, may contribute to changing DCD efficacy. Soil properties should be considered when tailoring DCD strategies for improving nitrogen use efficiency and crop yields, through the reduction of reactive nitrogen loss.     

Distribution of Inorganic and Organic Phosphorus Fractions in Two Phosphorus-Deficient Soils as Affected by Crop Species and Nitrogen Applications

It is desirable to know the distribution of phosphorus (P) fractions in soil so that plants may use P efficiently. Here we report the dynamics of inorganic and organic P in P-deficient black and rice soil cropped by soybean, white lupin, and maize supplied with nitrogen (N) inputs by N fixation and urea fertilizer. Inorganic P fractions of the three cropped soils could be ranked as O-P (organic phosphorus) > Al-P (aluminum phosphorus) > Fe-P (iron phosphorus) > Ca₁₀-P (calcium-10 phosphorus) > Ca₈-P (calcium-8 phosphorus) > Ca₂-P (calcium-2 phosphorus), irrespective of soil type. The potential of various inorganic P fractions to plant nutrition differed between soybean and white lupin. The percentage of total P present as inorganic P was affected by crop, soil type, and N source. In black soil, the change of organic P fraction induced by N fixation was larger than by urea application. The moderately labile organic P (MLOP) concentration was not affected significantly by soil type and crop species, and it was probably the main P source to the inorganic P fraction because the correlation between the two pools was high (r = 0.945; P < 0.05). Crop species differed in their uptake of inorganic and organic P from soil. Though P fraction concentrations varied between black soil and rice soil, their response to crop species and N source was similar. The amounts of P removed from soil were affected by N source. The right choice of crop species and the application a suitable N source may increase crop yield and P uptake by plant in P-deficient soils.     

Tree pruning mulch increases soil C and N in a shaded coffee agroecosystem in Hawaii

Agroforestry can increase the sequestration of carbon (C) in soils of tropical agroecosystems through increased litter and tree pruning inputs. Decomposition of these inputs is a key process in the formation of soil organic matter and in nutrient cycling. Our objectives were to study decay of tree pruning mulch and effects on soil C and N in a shaded coffee agroecosystem in Hawaii. Chipped tree pruning residues (mulch) were added to coffee plots shaded with the Leucaena hybrid KX2 over three years. We measured mulch decomposition and nitrogen loss over one year and changes in soil carbon and nitrogen (N) over two years. Mass loss of mulch was 80% over one year and followed first-order decay dynamics. There was significant loss from all major biochemical components. Net N loss from the mulch was positive throughout the entire period. The C:N and lignin:N ratios of the mulch declined significantly over the decomposition period. Mulch additions significantly increased soil C and N in the top 20 cm by 10.8 and 2.12 Mg ha⁻¹, respectively. In the no-mulch treatment, there was no significant change in soil C or N concentration, but a decline in soil bulk density led to a significant decline in total soil C. Leucaena mulch can provide an important source of organic C and N to coffee agroecosystems and can help sequester C lost as plant biomass during shade tree management.     

Asymbiotic biological nitrogen fixation in a temperate grassland as affected by management practices

Estimates of asymbiotic biological N fixation (BNF) in temperate grasslands are few with large variations. In the past six decades, European grasslands have been subjected to intensive management practices and presently it is not known how asymbiotic BNF is influenced by these practices. Our objective was to assess the impact of fertilizer application and mowing frequency on asymbiotic BNF in a Central European grassland. In 2008, we established a three-factorial experiment with two fertilizer treatments (no fertilizer application and combined nitrogen (N), phosphorus (P) and potassium (K) fertilization at 180–30–100 kg ha⁻¹ yr⁻¹), two mowing frequencies (cut once and thrice per year) and three sward compositions through the application of herbicides (control, monocot- and dicot-enhanced swards). Three years after the initial sward manipulation, there was no more difference in functional group composition. Between June 2011 and May 2012, we measured in-situ asymbiotic BNF using the acetylene reduction assay, calibrated with ¹⁵N₂-fixation method. Across treatments, asymbiotic BNF rates in the 0–5-cm soil depth ranged from 1.7 (±0.2 SE) kg ha⁻¹ yr⁻¹ for fertilized plots cut once a year to 5.7 (±2.3 SE) kg ha⁻¹ yr⁻¹ for unfertilized plots cut thrice a year. Fertilization decreased asymbiotic BNF, suggesting that the potential positive effect of increased soil P levels might have been overruled by the negative effect of increased soil mineral N levels. Intensive mowing stimulated asymbiotic BNF, which was probably due to an increase in rhizodeposition. Our calibration of the acetylene reduction assay with the ¹⁵N₂-fixation method resulted in a conversion factor of 0.61, which largely deviates from the theoretical conversion factor of 3. Furthermore, laboratory incubations under increased soil moisture and temperature conditions overestimated BNF rates compared to in-situ measurements. Thus, laboratory measurements with altered soil moisture, temperature or disturbed soil may lead to strong biases in estimates of asymbiotic BNF. Our results suggest that input of N through BNF may be considerable in temperate grasslands. We conclude that BNF studies should be conducted in-situ and that the acetylene reduction assay should be calibrated against ¹⁵N₂-fixation calibration for reliable estimates.     

Nitrogen release and mineralization potential of derivatives from nutrient recovery processes as substitutes for fossil fuel‐based nitrogen fertilizers

The need to meet rapidly increasing demands for synthetic nitrogen (N) while reducing dependence on fossil fuels has been driving widespread attention to the recuperation and reuse of nutrients present in digestate and animal manure. The N release and mineralization potential of animal manure (AM), digestate (DIG), liquid fraction of digestate (LFDIG) and mineral concentrate (MC) were assessed in comparison with N availability from calcium ammonium nitrate (CAN) as a reference. The release was highly dependent on the product ‐N/N_total ratio, while mineralization occurred only for products containing more than 5% of organic N. The magnitude of the released N, on average after 120 days of an incubation experiment, was in the order: CAN > MC > LFDIG > DIG ≥ AM. These results indicate that only the N release from MC exhibited patterns similar to CAN, suggesting that this product will provide plant available N in a similar fashion as synthetic fertilizers. The N release from LFDIG was higher than AM, but did not closely follow the pattern of CAN. The N availability in LFDIG may be increased using substrates richer in N, such as animal manure or waste food and not only plant residues.     

模拟氮沉降对华北落叶松人工林土壤微生物碳和微生物氮的动态影响

为揭示过量的大气氮沉降对华北落叶松人工林土壤微生物碳、氮和土壤呼吸的影响,通过对照(N0:0 g/(m^2·a))、轻度施氮(N1:8 g/(m^2·a))、重度施氮(N2:15 g/(m^2·a))3个外源施氮水平下5年的野外定点试验和观测,模拟过量氮沉降条件下华北落叶松人工林土壤微生物碳、氮和土壤呼吸的变化,旨在阐明林下土壤微生物和呼吸对过量氮沉降的响应及其对土壤碳氮循环的影响。结果表明:在5-10月生长季中,土壤微生物碳和氮的平均含量分别为1 098.93,97.31 mg/kg,二者都随土层深度的增加呈下降趋势;轻度施氮促进土壤微生物碳和氮的增加,重度施氮抑制土壤微生物碳和氮的增加;土壤微生物碳和微生物氮从生长初期5月起,5-7月呈增加趋势,7月出现峰值,8月降低,9-10月小幅增加,呈现"N"形曲线。土壤微生物碳氮比为4.94~18.54,且随施氮量增加而减小。各氮处理下,华北落叶松人工林土壤呼吸速率5,6月较低,7-8月持续增加,并在8月达到最高,9-10月逐渐降低。相关分析表明,土壤呼吸与土壤全氮、含水量、微生物碳和微生物氮含量呈极显著正相关关系,与土壤有机质呈显著正相关关系。在全球变化背景下,研究结果可为进一步明确过量大气氮沉降对森林生态系统碳氮循环的影响途径和机制研究提供重要参考。     

模拟氮沉降对湿性常绿阔叶次生林土壤碳氮组分和酶活性的影响

为了研究氮沉降对次生林土壤碳氮组分和酶活性的影响,以华西雨屏区湿性常绿阔叶次生林为对象,从2014年1月起进行野外定位模拟氮沉降试验,分别设置对照(CK,+0 g/(m^2·a))、低氮(LN,+5 g/(m^2·a))和高氮(HN,+15 g/(m^2·a))3个氮添加水平。在氮沉降进行27个月后,按照腐殖质层和淋溶层表层进行取样,测定不同土层土壤总有机碳(TOC)、可浸提溶解性有机碳(EDOC)、易氧化碳(ROC)、全氮(TN)、硝态氮(NO_3^-—N)和铵态氮(NH_4^+—N)含量以及蔗糖酶、脲酶、酸性磷酸酶和多酚氧化酶活性。结果表明:模拟氮沉降显著增加该次生林腐殖质层土壤的TOC和NH_4^+—N含量,显著增加腐殖质层和淋溶层表层土壤的NO_3^-—N含量,腐殖质层土壤C/N显著升高。淋溶层表层土壤TOC、NH_4^+—N、C/N以及2层土壤的EDOC、ROC、TN和NH_4^+—N/NO_3^-—N均无显著影响。2层土壤的多酚氧化酶活性均随着氮添加量的升高而降低,其中淋溶层表层达到显著差异。模拟氮沉降对蔗糖酶、脲酶和酸性磷酸酶活性均无显著影响。腐殖质层中,NH_4^+—N和NO_3^-—N含量与TOC含量存在极显著正相关关系。2层土壤的多酚氧化酶活性均与NO_3^-—N含量呈极显著负相关。结果说明,模拟氮沉降使该次生林中原本较高的腐殖质层土壤TOC含量进一步显著增加,并且促进土壤无机氮的积累,而模拟氮沉降对多酚氧化酶的抑制作用更加有利于土壤有机质的积累。     

Available organic nitrogen in temperate, subtropical, and tropical soils extracted with different solutions

Extraction of organic N by chemical solutions has been used to assess the amount of available N in soil. We tested the efficiency of several solutions in extracting organic N from tropical, subtropical and temperate soils. A conventional 0.067 M phosphate buffer successfully extracted organic N from all 23 soils examined. High-performance size exclusion chromatograms showed a single peak at about 7,800 Da for all phosphate buffer extracts irrespective of soil types. The peak area correlated with the organic N concentration of extracts. Tropical soils had lower retention of organic N than other soils according to the conventional and sequential extraction with phosphate buffer. Organic N extracted with sulfuric acid was significantly (P < 0.001) correlated with the amount of extracted Fe, suggesting that Fe might play a role in the retention of organic N in soil.