Pattern separation in the human hippocampal CA3 and dentate gyrus

Pattern separation, the process of transforming similar representations or memories into highly dissimilar, nonoverlapping representations, is a key component of many functions ascribed to the hippocampus. Computational models have stressed the role of the hippocampus and, in particular, the dentate gyrus and its projections into the CA3 subregion in pattern separation. We used high-resolution (1.5-millimeter isotropic voxels) functional magnetic resonance imaging to measure brain activity during incidental memory encoding. Although activity consistent with a bias toward pattern completion was observed in CA1, the subiculum, and the entorhinal and parahippocampal cortices, activity consistent with a strong bias toward pattern separation was observed in, and limited to, the CA3/dentate gyrus. These results provide compelling evidence of a key role of the human CA3/dentate gyrus in pattern separation.     

Requirement for hippocampal CA3 NMDA receptors in associative memory recall

Pattern completion, the ability to retrieve complete memories on the basis of incomplete sets of cues, is a crucial function of biological memory systems. The extensive recurrent connectivity of the CA3 area of hippocampus has led to suggestions that it might provide this function. We have tested this hypothesis by generating and analyzing a genetically engineered mouse strain in which the N-methyl-D-asparate (NMDA) receptor gene is ablated specifically in the CA3 pyramidal cells of adult mice. The mutant mice normally acquired and retrieved spatial reference memory in the Morris water maze, but they were impaired in retrieving this memory when presented with a fraction of the original cues. Similarly, hippocampal CA1 pyramidal cells in mutant mice displayed normal place-related activity in a full-cue environment but showed a reduction in activity upon partial cue removal. These results provide direct evidence for CA3 NMDA receptor involvement in associative memory recall.     

Pattern separation in the dentate gyrus and CA3 of the hippocampus

Theoretical models have long pointed to the dentate gyrus as a possible source of neuronal pattern separation. In agreement with predictions from these models, we show that minimal changes in the shape of the environment in which rats are exploring can substantially alter correlated activity patterns among place-modulated granule cells in the dentate gyrus. When the environments are made more different, new cell populations are recruited in CA3 but not in the dentate gyrus. These results imply a dual mechanism for pattern separation in which signals from the entorhinal cortex can be decorrelated both by changes in coincidence patterns in the dentate gyrus and by recruitment of nonoverlapping cell assemblies in CA3.     

Role of NMDA receptor subtypes in governing the direction of hippocampal synaptic plasticity

Activation of N-methyl-d-aspartate subtype glutamate receptors (NMDARs) is required for long-term potentiation (LTP) and long-term depression (LTD) of excitatory synaptic transmission at hippocampal CA1 synapses, the proposed cellular substrates of learning and memory. However, little is known about how activation of NMDARs leads to these two opposing forms of synaptic plasticity. Using hippocampal slice preparations, we showed that selectively blocking NMDARs that contain the NR2B subunit abolishes the induction of LTD but not LTP. In contrast, preferential inhibition of NR2A-containing NMDARs prevents the induction of LTP without affecting LTD production. These results demonstrate that distinct NMDAR subunits are critical factors that determine the polarity of synaptic plasticity.     

Asymmetrical allocation of NMDA receptor epsilon2 subunits in hippocampal circuitry

Despite its implications for higher order functions of the brain, little is currently known about the molecular basis of left-right asymmetry of the brain. Here we report that synaptic distribution of N-methyl-D-aspartate (NMDA) receptor GluRepsilon2 (NR2B) subunits in the adult mouse hippocampus is asymmetrical between the left and right and between the apical and basal dendrites of single neurons. These asymmetrical allocations of epsilon2 subunits differentiate the properties of NMDA receptors and synaptic plasticity between the left and right hippocampus. These results provide a molecular basis for the structural and functional asymmetry of the mature brain.     

Two gap genes mediate maternal terminal pattern information in Drosophila

In Drosophila three maternal pattern organizing activities, the anterior, the posterior, and the terminal, establish the anterior-posterior body pattern of the embryo by initiating the spatially restricted activities of the gap class of zygotic segmentation genes. The activities of tailless (tll) and the newly identified gap gene huckebein (hkb) are specifically involved in mediating the maternal terminal information at the posterior end of the blastoderm embryo.     

Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation

Calcium-calmodulin-dependent protein kinase II (CaMKII) is thought to increase synaptic strength by phosphorylating postsynaptic density (PSD) ion channels and signaling proteins. It is shown that N-methyl-D-aspartate (NMDA) receptor stimulation reversibly translocates green fluorescent protein-tagged CaMKII from an F-actin-bound to a PSD-bound state. The translocation time was controlled by the ratio of expressed beta-CaMKII to alpha-CaMKII isoforms. Although F-actin dissociation into the cytosol required autophosphorylation of or calcium-calmodulin binding to beta-CaMKII, PSD translocation required binding of calcium-calmodulin to either the alpha- or beta-CaMKII subunits. Autophosphorylation of CaMKII indirectly prolongs its PSD localization by increasing the calmodulin-binding affinity.     

Role for rapid dendritic protein synthesis in hippocampal mGluR-dependent long-term depression

A hippocampal pyramidal neuron receives more than 10(4) excitatory glutamatergic synapses. Many of these synapses contain the molecular machinery for messenger RNA translation, suggesting that the protein complement (and thus function) of each synapse can be regulated on the basis of activity. Here, local postsynaptic protein synthesis, triggered by synaptic activation of metabotropic glutamate receptors, was found to modify synaptic transmission within minutes.     

Contributions of quisqualate and NMDA receptors to the induction and expression of LTP

The contributions of two subclasses of excitatory amino acid transmitter receptors to the induction and expression of long-term potentiation (LTP) were analyzed in hippocampal slices. The quisqualate/kainate receptor antagonist DNQX (6,7-dinitro-quinoxaline-2,3-dione) blocked 85% of the evoked field potential, leaving a small response that was sensitive to D-AP5 (D-2-amino-5-phosphonopentanoate), an N-methyl-D-aspartate (NMDA) receptor blocker. This residual D-AP5-sensitive response was of comparable size in control and previously potentiated inputs. High-frequency stimulation in the presence of DNQX did not result in the development of robust LTP. Washout of the drug, however, revealed the potentiation effect. Thus NMDA-mediated responses can induce, but are not greatly affected by, LTP; non-NMDA receptors, conversely, mediate responses that are not needed to elicit LTP but that are required for its expression.     

The role of excitatory amino acids and NMDA receptors in traumatic brain injury

Brain injury induced by fluid percussion in rats caused a marked elevation in extracellular glutamate and aspartate adjacent to the trauma site. This increase in excitatory amino acids was related to the severity of the injury and was associated with a reduction in cellular bioenergetic state and intracellular free magnesium. Treatment with the noncompetitive N-methyl-D-aspartate (NMDA) antagonist dextrophan or the competitive antagonist 3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid limited the resultant neurological dysfunction; dextrorphan treatment also improved the bioenergetic state after trauma and increased the intracellular free magnesium. Thus, excitatory amino acids contribute to delayed tissue damage after brain trauma; NMDA antagonists may be of benefit in treating acute head injury.     

Artificial neural network models of the rumen fermentation pattern in dairy cattle

The objectives of this study were: (1) to predict the rumen fermentation pattern from milk fatty acids using a machine learning technique, i.e. artificial neural networks (ANN) combined with feature selection and (2) to compare the prediction accuracy of the resulting model to that of a statistical multi-linear regression model, based on odd and branched chain milk fatty acids. Data were collected from 10 experiments with rumen fistulated dairy cows, resulting in a dataset of 138 observations. Feature selection was based on correlation and principal component analysis, and background physiological knowledge. Different ANN architectures and training algorithms were assessed. The evaluation of the model performance, based on the test dataset, showed a root mean square prediction error, expressed relative to the observed mean, of 2.65%, 7.67% and 7.61% of the observed mean for acetate, propionate and butyrate, respectively. Compared to a multi-linear regression model, the ANN revealed not to perform significantly better. However, the results confirm that milk fatty acids have great potential to predict molar proportions of individual volatile fatty acids in the rumen.     

“分散式”:农业信息网络建设的一种新模式

本文通过介绍一个目前渐成规模的农业信息网站WISARD及其建设背景 ,分析了其“分散式”网络建设模式的特点和对我国农业信息网络建设的启示     

A rapid shift in a classic clinal pattern in Drosophila reflecting climate change

Geographical clines in genetic polymorphisms are widely used as evidence of climatic selection and are expected to shift with climate change. We show that the classic latitudinal cline in the alcohol dehydrogenase polymorphism of Drosophila melanogaster has shifted over 20 years in eastern coastal Australia. Southern high-latitude populations now have the genetic constitution of more northerly populations, equivalent to a shift of 4 degrees in latitude. A similar shift was detected for a genetically independent inversion polymorphism, whereas two other linked polymorphisms exhibiting weaker clinal patterns have remained relatively stable. These genetic changes are likely to reflect increasingly warmer and drier conditions and may serve as sensitive biomarkers for climate change.     

Dual activation of the Drosophila toll pathway by two pattern recognition receptors

The Toll-dependent defense against Gram-positive bacterial infections in Drosophila is mediated through the peptidoglycan recognition protein SA (PGRP-SA). A mutation termed osiris disrupts the Gram-negative binding protein 1 (GNBP1) gene and leads to compromised survival of mutant flies after Gram-positive infections, but not after fungal or Gram-negative bacterial challenge. Our results demonstrate that GNBP1 and PGRP-SA can jointly activate the Toll pathway. The potential for a combination of distinct proteins to mediate detection of infectious nonself in the fly will refine the concept of pattern recognition in insects.     

网络环境下信息服务模式初探

本文介绍了网络环境下信息服务的特点及网络化信息服务模式，分析了网络化信息服务的未来发展趋势，并指出我国发展网络化信息服务的对策。     

快速交通对城市郊区獐的生境影响分析

掌握城市郊区的野生动物生境动态变化,对于制定野生动物保护策略具有重要的理论和现实意义。以南京东郊野生哺乳动物獐Hydropotes inermis为研究对象．以2006年中巴资源卫星、2011年HJ-1A小卫星、47个獐物种痕迹点数据为主要信息源．在南京东郊快速交通网络景观格局动态变化分析基础上．采用最大熵法Maxent生态位模型．进行快速交通对城市郊区大型哺乳动物生境影响分析。结果表明：2006—2011年。南京东郊快速交通网络日益复杂．道路密度从2006年的1116．27m·km-2增加到2011年1371．95m·km-2：快速交通修建对獐生境产生囚笼效应．导致獐的潜在生境呈孤岛状空间分布格局。随着快速交通网络日益复杂,与2006年相比,2011年獐潜在生境呈缩小趋势：獐生境概率分布冷热点分析表明．距道路和居民点距离这2个人为干扰因子对獐生境概率分布影响最大。图4表2参11     

我国九亿农民网络信息传递方式的探索

我国农业面临着一场深刻的变革，农村信息传递手段的落后，制约着农民难以参与这场变革。为使农民以较高的科技素质和市场竞争力迎接农业革命的挑战，河南农科院信息所近年来加强了农业网络建设和网上信息资源建设，探索了网络环境下各种类型的适合于农村的信息传递方式，有效地解决了农民的信息需求。     

快速城市化影响下超大型城市景观生态格局演变特征分析

针对超大型城市建设对景观生态格局及其变化定量研究的应用需求,基于多时相遥感变化监测和格网划分方法,结合景观指数对北京景观空间格局及动态变化进行定量分析,同时探究城市化对城市景观影响的阈值效应。结果表明:1)2000—2015年,由于北京城市化水平逐渐提高,导致建筑用地增加了56%,斑块破碎程度较低;而城市耕地及绿地景观有所减少、斑块分布不均,破碎程度上升;2)空间格局方面,由于建筑用地的覆盖及彼此间的高度连接,使城区景观破碎程度明显低于城郊,景观多样性水平呈下降趋势;3)回归分析表明城市化对城市景观破碎度有显著影响,相关系数达0.84,具体表现为倒U关系,即景观破碎度随城市化水平的提高呈先上升后下降的关系,城市化水平在51%～53%时达到峰值。     

A role for the macaque anterior cingulate gyrus in social valuation

Complex human social interaction is disrupted when the frontal lobe is damaged in disease, and in extreme cases patients are described as having acquired sociopathy. We compared, in macaques, the effects of lesions in subdivisions of the anterior cingulate and the orbitofrontal cortices believed to be anatomically homologous to those damaged in such patients. We show that the anterior cingulate gyrus in male macaques is critical for normal patterns of social interest in other individual male or female macaques. Conversely, the orbitofrontal cortex lesion had a marked effect only on responses to mildly fear-inducing stimuli. These results suggest that damage to the anterior cingulate gyrus may be the cause of changes in social interaction seen after frontal lobe damage.