Management of Italian and Perennial Ryegrasses for Seed and Forage Production in Crop Rotations

Italian ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (L. perenne L.) can be grown for seed and forage in cold winter regions provided the stand persists well over winter. Seed yield and plant characteristics during primary growth, and forage yield during regrowth, were determined for two Italian and one perennial ryegrass cultivars in Atlantic Canada. Establishment methods and dates included sowing ryegrass in cultivated soil alone or with barley in mid‐May and, after harvesting the barley crop, by sowing ryegrass following conventional or reduced cultivation and by no‐till drilling into barley stubble in mid‐August and early September. Despite some winterkill, particularly in Italian ryegrass, seed and forage yields were adequate in post‐establishment growing seasons. Seed yield for Italian ryegrass was greatest (1270 kg ha^?1) when it was sown into cultivated soil in mid‐August and least (890 kg ha^?1) when sown alone in May. Italian ryegrass yielded 15–17 % more seed when plots were established in mid‐August rather than in mid‐May or early September. Italian ryegrass cv. Lemtal had a greater density of fertile tillers (1030 m^?2) in the sward than cv. Ajax (860 m^?2) and its tiller density was greater when seeded into cultivated soil in September than in mid‐August. There were fewer spikelets per seed head for sowing Italian ryegrass with barley in May than for the other methods of establishment. Forage yield in regrowth was greater for Italian ryegrass cv. Ajax (2770 kg ha^?1) than for cv. Lemtal (2480 kg ha^?1). Seed yield of perennial ryegrass was greater when seeded in mid‐May than in mid‐August or early September. The seed yield of perennial ryegrass was greater when it was sown with barley in May and harvested for grain, than when it was sown alone or with barley harvested at late milk stage. The establishment methods for mid‐August and early September sowing had little effect on seed yield. However, the no‐till and reduced tillage methods resulted in a greater tiller density than sowing into the cultivated seedbed. Fertile tillers tended to be denser under reduced cultivation for sowing in August. Forage yield of perennial ryegrass regrowth was not influenced by the sowing method and timing. In conclusion, Italian and perennial ryegrasses produce adequate seed and forage regrowth under different establishment methods and timing. However, the poor persistence of Italian ryegrass may limit commercial production after the establishment year in Atlantic Canada.     

Assessment of Cattle Preference for Perennial Ryegrass Varieties in Association with White Clover. Does White Clover Content Interfere?

There is a need to evaluate grass varieties under grazing management to allow selection of varieties to increase animal performance. However, the evaluation of animal performance under grazing with each variety is arduous and time-consuming. An alternative is to describe the preference by grazing animals when given a choice of different varieties. Indeed, there is some indications that preference is related to variety characteristics such as leaf content or digestibility, characteristics having also an impact on the level of intake of this variety. However, preference for grass varieties, when sown alone, may not reflect agricultural practice as grasses often occur in mixed swards, associated with white clover. So, the aim of the present work was to determine whether, under grazing management, cattle expressed selections when given a choice of mixed swards in which different perennial ryegrass varieties were grown in association with a common white clover variety at two levels of fertilizer application (60 or 160 kg N ha⁻¹ annum⁻¹). The preference for perennial ryegrass variety per se was then assessed after taking white clover content into consideration. The results show that there were significant differences in preference for perennial ryegrass varieties, per se, even for varieties with the same heading date and ploidy. The effect of white clover cover on sward selection was more marked in swards which received the higher level of nitrogen fertilizer application which may have been related to a more variable white clover content between swards. This suggests that, under low nitrogen fertilizer application, differences in perennial ryegrass varieties preference can be determined in the same experiments as are used to determine ryegrass varieties persistence in association to white clover. Nevertheless, before such ‘variety preference’ tests can be applied in practice, a validation of the possible correlation between ‘variety preference’ and ‘variety intake’ will be necessary.     

Contribution of White Clover Varieties in High-Productivity Systems under Grazing and Cutting

Two medium leaf size varieties of white clover (Trifolium repens L.) were grown in mixture with a range of grass species and varieties in a field experiment conducted over 4 years. The clovers were grown singly or as a blend in swards with a tetraploid perennial ryegrass (Lolium perenne L.), a diploid Italian ryegrass (Lolium multiflorum L.) or tetraploid hybrids between these two species (Lolium x boucheanum Kunth). These grasses were also grown in monoculture. Swards were managed in a regime involving two periods of sheep grazing, two periods of cattle grazing and a silage cut in the same year for 4 years. The objectives of this experiment were 3‐fold: (1) to examine the yield and persistency of white clover in highly productive systems with modern perennial, hybrid and Italian ryegrasses; (2) to compare the performance of white clover varieties grown singly in mixture with grasses with their performance together as a blend, and (3) to examine the persistency of new Italian ryegrass varieties, exemplified by AberComo. The clover content of the sward remained high throughout the experiment, with a mean of 31 % overall. Differences in clover yield between plots were largely attributable to the effects of companion grasses. Total yields were very similar in plots containing different clover varieties and in plots where these varieties had been grown singly or as a blend. Grass yields in mixture were greater than those in monoculture but showed similar relative rankings. The diploid Italian ryegrass variety AberComo showed an unexpectedly high yield in the 4th year of the experiment. The implications of these results for strategies for productive, sustainable agriculture using modern white clover and ryegrass varieties are discussed.     

Winter cover crop: the effects of grass–clover mixture proportion and biomass management on maize and the apparent residual N in the soil

The advantages and disadvantages of varying mixture proportion of crimson clover (Trifolium incarnatum L.) and Italian ryegrass (Lolium multiflorum Lam.), used as winter cover crops, and cover crop biomass management before maize sowing (Zea mays L.) were studied in a series of field experiments in Eastern Slovenia. Pure stands and mixtures of cover crops on the main plots were split into different cover crop biomass management subplots: whole cover crop biomass ploughed down before maize sowing, aboveground cover crop biomass removed before ploughing and sowing, or aboveground cover crop biomass removed before sowing directly into chemically killed residues.Cover crop and cover crop biomass management affected the N content of the whole aboveground and of grain maize yields, and the differences between actual and critical N concentrations in the whole aboveground maize yield. The whole aboveground and grain maize dry matter yields, and the apparent remaining N in the soil after maize harvesting, showed significant interaction responses to cover crop × management, indicating positive and negative effects. Crimson clover in pure stand provided high, and pure Italian ryegrass provided low maize dry matter yields and N content in the yields in all the observed methods of biomass management. However, within individual management, mixtures containing high proportions of crimson clover sustained maize yields and N contents similar to those produced by pure crimson clover. Considering the expected ecological advantages of the mixtures, the results thereby support their use.     

The Effects of Different Traditional Sources of Nutrients on the Infestation of Pepper Fruits by the Pepper Fruitfly, Atherigona orientalis (Schiner), in Nigeria

Experiments were conducted in 1995 and 1996 to determine the effects of different sources of nutrients on the infestation of fruits of five pepper varieties by Atherigona orientalis (Schiner). Different sources of nutrients — chicken droppings, plant residues (compost manure), wood ash and NPK (chemical fertilizer) — were used in the planting of each of the five pepper varieties [Nsukka yellow, Atarugu, and Sweet pepper, all of Capsicum annum; Bird's eye chilli and Local medium red (Ogoni pepper) both of C. frutescens ]. In 1995 plantings, pepper plots of Nsukka yellow and Atarugu varieties that received chicken droppings as source of nutrients suffered the highest percentage fruit damage of 90.7 % and 80.8 %, respectively. Pepper plots of Nsukka yellow, Atarugu, Sweet pepper and Local medium red grown in 1996 and treated with chicken droppings suffered the highest percentage fruit damage of 93.7 %, 56.6 % and 52.2 %, respectively. Plots of the Bird's eye chilli variety received minimal or no fruit damage in all. Pepper fruits in the control plots also did not undergo A. orientalis attack. Chicken droppings offered the lowest Relative Protection (RP) values to most pepper varieties in 1995 and 1996. NPK chemical fertilizer offered the highest percentage RP values in 1995 and wood ash offered the highest RP in 1996 to most pepper varieties. Also, wood ash in both 1995 and 1996 offered a relatively high percentage RP to most pepper varieties. Compost manure offered a moderate percentage RP in both years.     

Symbiotic Nitrogen Fixation of White Clover in a Mixed Sward is not Limited by Height of Repeated Cutting

The aim of this study was to investigate whether symbiotic nitrogen fixation in white clover nodules limits nitrogen supply and hence clover growth by repeated defoliation at two cutting heights. Other possible factors governing symbiotic nitrogen fixation in the field were also elucidated. Using ¹⁵N, a 2-year field experiment including white clover ( Trifolium repens L. cv. Ladino) and perennial ryegrass ( Loliumperenne L. cv. Bastion) in monocultures and in mixtures was conducted in Eschikon, Switzerland. The effect of two cutting heights (4 cm and 10 cm above ground level) on the performance of symbiotic nitrogen fixation of white clover in the different sward-types was investigated. After each harvest, the plots were fertilized with 3 g N m^-2(equivalent to 30 kg N ha^-1 cut^-1 or 210 kg N ha^-1 year^-1). In both years, white clover grown in a mixture with grass received a significantly higher percentage of nitrogen from symbiotic fixation compared with clover grown in monoculture. This phenomenon is attributed to the strong competitiveness of ryegrass in soil nitrogen uptake. Consequently, white clover in the clover-ryegrass mixture was more dependent on symbiotic nitrogen fixation than that grown in monoculture. The cutting height did not preferentially influence symbiotic nitrogen fixation, as opposed to the uptake of mineral nitrogen from the soil. From this finding it is suggested that symbiotic nitrogen fixation did not limit the supply of nitrogen to clover and hence its growth. It is proposed that symbiotic nitrogen fixation in white clover is regulated by the demand for nitrogen rather than by the availability of carbohydrate reserves in the stolons. Symbiotic nitrogen fixation should thus be looked upon as an integrated plant growth factor and not as an isolated phenomenon.     

Intercropping of Corn with Soybean, Lupin and Forages: Silage Yield and Quality

Intercropping of corn with legumes is an alternative to corn monocropping and has a number of advantages, for example, lower levels of inputs, lower cost of production and better silage quality than monocrop systems. An experiment was carried out at two sites in 1993 and 1994 to investigate the effects of seeding soybean or lupin alone or in combination with one of three forages (annual ryegrass, Lolium multiflorum Lam.; perennial ryegrass, Lolium perenne L.; red clover, Trifolium pratense L.) on silage yield and quality. The intercrop plots received 90 kg ha⁻¹ less nitrogen fertilizer than monocrop plots, which received 180 kg ha⁻¹. Corn biomass yield had a variable response to the treatments, but showed no change at most site-years. Soybean and lupin biomass yields were decreased by intercropping (80–98 % for soybean, and 94–100 % for lupin). However, when corn growth was limited due to poor establishment at one site in 1994, soybean was able to grow well and produce yields similar to those of monocropped soybean. The three underseeded forages did not grow well during the period examined (up to silage harvest) and had no effect on the yield of any crop. Total silage yields were similar to corn monocrop biomass yields even during 1994 at the site with low corn population densities because soybean was able to compensate for reduced corn growth.     

Useful variation in in vitro digestibility within perennial ryegrass

In vitro dry matter digestibility (DMD) and dry matter yield (DMY) of herbage from plots sown with three selected perennial ryegrass varieties with similar heading dates (Ba11778, Talbot and Gator) under a range of harvesting frequencies (2, 3, 4 and 5-weekly harvests) were compared over two harvest years (1994 and 1995). Varieties varied in mean percentage DMD over all harvests and harvesting frequencies by 3.4 and 3.5 units in 1994 and 1995 respectively. Varietal ranking in mean DMD over all harvests was the same under each harvesting frequency in both years: Ba11778 being the highest, Gator the lowest and Talbot intermediate. In 1995, Gator had a higher proportion of unsown species in the herbage than both Ba11778 and Talbot but this was only a minor factor contributing to the differences among the varieties in mean DMD over all harvests. Although varieties consistently ranked in the same order of mean DMD over all harvesting frequencies during 13 5-week periods, the range among varieties varied from less than 1 unit to 8.6 units. This range was greatest in mid-season of each year when mean DMD over all varieties was lowest. The minimum mean DMD over all harvesting frequencies during both years was 75.5%, 70.9% and 66.8% for Ba11778, Talbot and Gator respectively. The most digestible variety Ba11778 also had higher annual DMY than the other two varieties in both harvest years and under all four harvesting frequencies. These results indicate that there is sufficient useful genetic variation for DMD within perennial ryegrass to justify breeding for the trait and its routine assessment in official variety trials.     

The Persistence of Tetraploid Perennial Ryegrass in a Mixture with Diploid Perennial Ryegrass

An investigation was carried out into the survival of two tetraploid varieties of perennial ryegrass ( Lolium perenne L.), sown in a mixture with a diploid and very intensively grazed for over five years.
Mixture M1 consisted of the late heading varieties Meltra and Vigor , and mixture M2 of the early heading varieties Merlinda and Melino.
The ploidy was determined by microscopic analysis.
The share of the tetraploid component decreased in both mixtures. The decrease was more pronounced in the early heading mixture. Information was also sought on the technique of sampling such swards.     

Cover Crops for Saline Soils

A 3-year study was conducted in the Central Valley of California to evaluate 125 prospective winter-growing cover crops for growth and nitrogen productivity in saline soils. Soil saturation paste electrical conductivities (ECes) in the surface 15 cm averaged 7 dS  m⁻¹ at fall planting and 5.3 dS m⁻¹ at spring harvest dates of each experiment. Species evaluated varied substantially in plant height. In general, the tallest plants were the Brassica species, which consistently grew to over 1.4 m. Annual grasses (barley, rye, triticale and wheat) averaged about 1.0–1.3 m in each year. Of the legume species screened, heights were greatest for Hedysarum coronarium , Trifolium alexandrium , Vicia spp., and Medicago polymorpha and truncatula , averaging 59, 47, 39, 38 and 37 cm, respectively, over all experiments. About one third of the species screened produced crop cover in excess of 90 % in each year. Groups of plants with consistently high crop cover percentages included various species/accessions of Brassica , Hedysarum , annual grasses, cool-season annual medics, Medicago polymorpha and Medicago truncatula , and two annual clovers, Trifolium alexandrium cv 'Multicut' and rose clover, Trifolium hirtum cv 'Hykon.' Total above-ground plant dry weights were highest for Brassica spp., which produced twice as much biomass as the annual grass species, and roughly four times as much dry matter as any of the legume species. Hedysarum , Lana and Namoi woolypod vetch, purple vetch, berseem clover, and several of the annual medic species consistently had the highest biomass among the legumes.     

Intercropping corn with soybean,lupin and forages: yield component responses

Intercropping systems influence yield variables of the component crops, such as harvest index, hundred seed weight, number of reproductive organs and number of seeds, within each reproductive unit. Two experiments were carried out at each of two sites during 1993 and 1994. The first experiment investigated the effects of seeding soybean or lupin alone or in combination with one of three forages (annual ryegrass, Lolium multiflorum Lam.; perennial ryegrass, Lolium perenne L.; red clover, Trifolium pratense L.) with corn on the yield components of corn, soybean and lupin. The second experiment examined the effects of seeding date (simultaneous with corn or 3 weeks later) and number of rows of large seeded legumes (one or two) seeded between the corn rows. Corn grain yield was generally not affected by any intercrop treatment, although in 1993 some simultaneously seeded treatments resulted in decreased yields. Soybean grain yield was decreased by most treatments, although some simultaneous seedings produced yields similar to soybean monocrops. Lupin grew poorly as an intercrop component, producing little or no grain. Corn harvest index was not affected by any intercrop treatments. Seeding corn and large-seeded legumes simultaneously resulted in decreases in corn hundred seed weights by as much as 6.6 g compared with the monocropped corn. In 1993 (a year with normal precipitation levels), the hundred seed weight and number of seeds per soybean pod were decreased by intercropping, although the harvest index was not affected. In a high precipitation year (1994), the soybean harvest index was decreased by intercropping, but not the seed components. The underseeded forages, annual ryegrass, perennial ryegrass and red clover, had no effect on yields or yield components of the other intercropped species.     

Selection for yield in Lolium perenne. II. performance of spaced plant selections under competitive conditions

Summary The performance of six lines of perennial ryegrass (Lolium perenne L.) which had been selected for three generations for production under spaced plant conditions were assessed under increasing levels of competition, namely spaced plants, drills, miniplots and large sown plots. Successful selection for yield under spaced plant conditions was not accompanied by comparable production patterns in the alternative managements. The extreme situation occurred in swards where a significant decline in production was encountered. Negative or no relationships were found in the establishment year between spaced plant production and yield in the alternative conditions whereas in the first harvest year positive correlations occurred between spaced plants, drills and miniplots and only the latter with plots. These varying patterns of performance are considered in relation to the prediction of sward behaviour and the appropriate environment for selection.INIA. CRIDA 01, La Coruna, Spain.     

行间生草对秦岭北麓猕猴桃园土壤养分及pH的影响

[目的]通过对秦岭地区猕猴桃园进行行间生草,来探讨多年生草对土壤pH、有机质及养分的影响。[方法]在西安猕猴桃试验站4年生的海沃德种植园,设置间作黑麦草、三叶草和林间组合,以自然生草为对照,于种植后第1、第2和第3年萌芽期分别测定果园不同土层的土壤pH、有机质、全氮、全磷、全钾、速效磷、速效钾含量的变化,并采用方差分析对数据进行分析。[结果]随着生草年限的递增,通过生不同的草种,土壤中全氮、有机质含量整体呈递增趋势;当生草年限达到3年时,随着土壤深度的增加,土壤中速效磷、速效钾含量逐渐降低;随着生草年限的递增,不同土层中自然生草的pH先增加后降低,三叶草的pH先降低后增加,黑麦草和林间组合的pH逐渐降低。[结论]在猕猴桃园生草能改良土壤养分状况,提高土壤有机质含量调节土壤pH。不同草种对土壤pH的影响不同,行间生林间组合和黑麦草能够改良北方的盐碱地。     

Improved persistence of red clover (Trifolium pratense L.) increases the protein supplied by red clover/grass swards grown over four harvest years

UK livestock agriculture can significantly reduce its protein imports by increasing the amount of forage based protein grown on-farm. Forage legumes such as red clover (Trifolium pratense L.) produce high dry matter yields of quality forage but currently available varieties lack persistence, particularly under grazing. To assess the impact of red clover persistence on protein yield, diploid red clover populations selected for improved persistence were compared with a range of commercially available varieties. All populations were grown over four harvest years in mixed swards with either perennial ryegrass (Lolium perenne L.) or perennial plus hybrid ryegrass (L. boucheanum Kunth). Red clover and total sward dry matter (DM) herbage yields were measured in Years 1–4, red clover plant survival in Years 3 and 4 and herbage protein (CP) yield and concentration in Years 2 and 4. In general, red clover DM yield in year 4 (3.4 t ha⁻¹) was lower than in year 1 (13.9 t ha⁻¹) but the red clover populations differed in the extent of this decline. Differences in the persistence of the red clover populations in terms of plant survival and yield were reflected in the contribution of red clover to the total sward yield in Year 4, which ranged from 61% for the highest yielding population, AberClaret, to 11% in the lowest yielding, Vivi. Increased red clover DM yield was reflected in a greater CP yield (protein weight per unit area), which ranged from 1.6 t ha⁻¹ year⁻¹ to 2.9 t ha⁻¹ year⁻¹ in Year 2 and from 1.1 t ha⁻¹ year⁻¹ to 1.9 t ha⁻¹ year⁻¹ in Year 4. CP concentration (protein weight per unit herbage weight) of all of the red clover populations was within a range considered suitable for ruminant production. The implication of these results for the future use of red clover in sustainable grassland systems is discussed.     

The use of hedges to reduce winter wheat losses caused by Italian ryegrass (Lolium multiflorum)

We hypothesized that short duration plant interference aiming to change mainly light intensity and red:far red ratio during crop establishment in Italian ryegrass-infested winter wheat fields, could hinder the development of Italian ryegrass and thus reduce its effect on wheat yield. To test this hypothesis, wheat was planted between previously established live (low red:far red ratio) or dead (high red:far red ratio), barnyardgrass or maize, plant hedges. Experimental results demonstrated that the presence of live or dead plant hedges during the first 30 days of crop establishment and also hedge orientation were important factors regulating weed and crop biomass production and competitive relationships. In barnyardgrass hedge treatments wheat yield was improved up to 67% and Italian ryegrass production was reduced by more than 20%. In plots with maize live hedges oriented N–S the biomass production of wheat was independent of production of Italian ryegrass. Data presented here suggests that there is room for developing weed control technologies on the basis of understanding photosensory processes of weed and crop species.     

Establishment, Development and Yield of Forage Legumes and Grasses as Cover Crops in Grain Corn in Eastern Canada

Interseeded cover crops can minimize soil erosion, increase organic matter and nutrient levels and the yield of subsequent crops. However, their performance is very sensitive to local conditions. Field experiments were conducted at two Quebec locations in 1993 and 1994 in order to evaluate the potential use of forage legumes and grasses as interseeds in corn in eastern Canada. Twelve forage species were evaluated. Fall rye ( Secale cereal L.), hairy vetch ( Vicia villosa Roth), a mixture of red clover ( Trifolium pratense L.) and ryegrass ( Lolium multiflorum Lam), a mixture of white clover ( Trifolium repens L.) and ryegrass, subterranean clover ( Trifolium subterraneum L.), yellow sweet clover ( Melilotus officinalis Lam.), black medic ( Medicago lupulina L.), Persian clover ( Trifolium resupinatum L.), strawberry clover ( Trifolium fragiferum L.), crimson clover ( Trifolium incarnatum L.), annual alfalfa ( Medicago saliva L.) and berseem clover ( Trifolium alexandrinum L.) were seeded at two planting dates (10 and 20 days after corn emergence). The control treatments were: hand weeding, chemical weeding and non-weeded. Early seeded forages established better and had higher biomass accumulation than the late seeded ones. In the presence of larger weed populations, the interseeded forages did not develop well due to competition with the weeds. At Macdonald crimson clover provided good soil cover while Persian clover, fall rye and alfalfa provided relatively little cover. Strawberry clover and hairy vetch did not provide early ground cover due to their late development. Forage mixtures of red or white clover and rye grass established well and achieved high populations at the end of the growing season. Fall rye provided good early ground cover but senesced by the middle of the season. The better establishment and early germination of crimson clover caused a 19% reduction in corn grain yield in 1993. In 1994 none of the cover crops caused a reduction in corn yield.     

Temperature Before Cold Acclimation Affects Cold Tolerance and Photoacclimation in Timothy (Phleum pratense L.), Perennial Ryegrass (Lolium perenne L.) and Red Clover (Trifolium pratense L.)

The expected temperature rise in late summer/early autumn can change the conditions for acclimation and affect the winter survival of perennial crops. This study examined the effect of the temperature just before the onset of cold acclimation (pre‐acclimation) on freezing tolerance of timothy (Phleum pratense L.), perennial ryegrass (Lolium perenne L.) and red clover (Trifolium pratense L.) populations (both cultivars and breeding populations) adapted to either northern or southern parts of Norway. Using phytotron experiments, we studied whether increasing pre‐acclimation temperature delays growth cessation, affects photoacclimation and reduces freezing tolerance. Furthermore, we assessed whether these effects were related to the latitudinal adaptation of the plant material. The results showed that a rise in pre‐acclimation temperature decreased both cold acclimation capacity and photoacclimation in these species. This affected the freezing tolerance, which was reduced significantly more in northern‐adapted population of timothy and perennial ryegrass compared with southern‐adapted populations. Red clover was less affected by temperature changes than the grasses.     

Response of Mustard (Brassica juncea L.) to Applied Nitrogen with or without Ethrel Spray under Non-irrigated Conditions

Field studies were conducted during the winter seasons of 1995–96 and 1996–97 at the Agricultural Farm of Aligarh Muslim University, Aligarh, India on mustard ( Brassica juncea L. Czern & Coss., var. Alankar) under non-irrigated conditions, to evaluate the effect of foliar spray of 200 p.p.m. ethrel (2-chloroethyl phosphonic acid) at flowering growth stage along with basal 0, 40, 80 or 120 kg N ha⁻¹ on net photosynthetic rate (P_N), stomatal conductance (C_S), stomatal resistance (R_S), leaf K content, relative water content (RWC), leaf area index (LAI) and total dry matter (TDM) production monitored at 20 days after spray application, and plant N content, seed N content, nitrogen harvest index (NHI), nitrogen yield merit (NYM), pods plant⁻¹, 1000 seed weight, seed yield, biological yield, harvest index (HI), seed yield merit (SYM) and merit of genotype (MOG) at harvest. Results indicated that, at 0 or 40 kg N ha⁻¹, ethrel did not produce any significance effect, but at basal 80 kg N ha⁻¹, ethrel affected the parameters favourably with the exception of 1000 seed weight, HI, seed N and NHI. Ethrel-sprayed plants utilized N from the soil more effectively and showed increased NYM. Yield attributes, seed yield and merit of genotype (in terms of NYM and SYM) were also enhanced. Ethrel spray enhanced seed yield under water stress conditions mainly by increasing K uptake and retaining higher RWC, thereby decreasing R_S and increasing LAI, P_N and TDM production.     

Endophyte Infection May Affect the Competitive Ability of Tall Fescue Grown with Red Clover

Cool-season grasses infected with Neotyphodium endophytes may be more persistent and competitive than non-infected plants. In a greenhouse experiment, two tall fescue ( Festuca arundinacea Schreb.) cloned genotypes (DN2 and DN11) with different growth characteristics and endophyte status (E−, non-infected; E+, infected) were grown in monocultures and in full competition with red clover ( Trifolium pratense L.) cv. Reddy Red for 20 weeks. When grown in monoculture, endophyte infection reduced the root dry matter (DM) of DN2 (16 %) and DN11 (5 %) when compared to E− plants, while shoot DM was not affected. In full competition with red clover, root and shoot DM of tall fescue were not influenced by endophytes, but cumulative herbage DM yield was less in E+ than in E− plants. Root growth of red clover was significantly depressed (22 %) in competition with E+ plants of DN2 compared to E− plants, but was not affected when plants were grown in competition with DN11. The relative yield total (RYT) did not differ from 1.0 in red clover mixtures with E− plants of DN2 and DN11, and with E+ plants of DN11, indicating competition between the competitors for the same resources. In contrast, RYT was less than 1.0 in the red clover/DN2 E+ mixture, suggesting a negative interaction between the competitors. Endophyte infection increased the competitive ability of DN2 but reduced that of DN11 when compared to E− plants. Because the concentration of pyrrolizidine alkaloids in roots of E+ plants of DN2 was twice that found in roots of DN11 (1083 and 536 μg g⁻¹, respectively), we suggest that pyrrolizidine alkaloids might influence the competitive ability of some endophyte–tall fescue associations through a possible allelopathic effect on companion species.