Small-scale hatcheries in north-east Thailand

Abstract. The development of small-scale hatcheries is placed in perspective with olher commercial methods of fish seed production, private and public, in the north-east region of Thailand. Case studies of six small-scale hatcheries operated by farmers in this region are presented with details of the production, costs and returns of these farms in relation to their size, location, species of fish produced, amount of capital invested and marketing and sales strategies, Rates of return were found to be favourable, with the exception of a group-based hatchery. The relationship between the production of carp and tilapia fry. which is believed tn be incompatible under present systems, is discussed. The wider implications of the role of small-scale hatcheries to provide fish seed for farmers in rural areas and the future of public sector involvement are considered.     

Design and Cost Analysis of a Self‐contained Mobile Laboratory for Commercial‐scale Aquatic Species Cryopreservation

Although aquatic species cryopreservation protocols have been studied around the world over the past 60 yr., germplasm repository development efforts and commercialization have begun only recently. The goal of this project was to develop a self‐contained mobile laboratory for on‐site high‐throughput cryopreservation of aquatic species. The objectives of this study were to: (1) identify how a mobile laboratory would function in different operational scenarios, (2) customize an enclosed cargo trailer to function as a mobile laboratory, (3) evaluate the laboratory layout and ability of cryopreservation equipment to operate from generator power, and (4) document the investment costs for private and public groups to integrate a mobile laboratory into an existing cryopreservation facility at three levels of automation and estimate the total cost per trip based on hypothetical assumptions for two scenarios (aquaculture production and repository development). There were three operational designs identified for the mobile laboratory: (1) self‐contained work inside the unit using generator power, (2) work inside the unit using external facility power, and (3) using the equipment inside of a host facility. The investment costs for a base‐level mobile laboratory ranged between US$5670 and US$5787 for private groups and between US$5208 and US$5315 for public groups. With the addition of a range of automated processing equipment, total investment costs ranged from US$13,616 to US$103,529 for private groups and US$12,494 to US$94,891 for public groups. The total cost per trip to cryopreserve sperm of 59 blue catfish, Ictalurus furcatus, males to produce 6300 0.5‐mL French straws was estimated to range from US$6089 to US$14,633 for private and between US$5703 and US$16,938 for public groups depending on the level of automation. Total cost per trip to cryopreserve sperm of 500 males of five different species in the genus Xiphophorus to produce 641 0.25‐mL French straws was estimated to range from US$6653 to US$7640 for private and US$7582 to US$8088 for public groups depending on level of automation. Overall, a commercial‐scale mobile laboratory was developed that can assist current germplasm activities and support future repository and industry development, and the layout information provided can help others to design and build comparable units.     

The Cost of Production of Giant Clam Seed Tridacna gigas

The costs of providing giant clam seed in Australia are examined for alternative annual volumes of production. Considerable economies of scale in production are available, both in relation to labor costs and non-labor costs (mostly capital costs). The fall in per-unit cost of producing giant clam seed is considerable when annual production is expanded from 100,000 to 500,000 seed clams per year. At 10% rate of interest, the unit cost per clam seed falls from $1.43–$2.04 at a production level of 100,000 to $0.41–$0.55 at a production level of 500,000. Per-unit operating costs also fall. They decline from $1.01–$1.22 to $0.29–$0.35. This suggests that there are likely to be cost economies in having large centralized hatcheries.     

Economies of scale for juvenile production of common snook (Centropomus undecimalis Bloch)

Abstract

Construction and operating costs for three common snook (Centropomus undecimalis) hatcheries in Florida, USA, are illustrated. Hypothetical capacities and targets of the three hatcheries (Scenarios I, II and III respectively) were 615, 000 juveniles (8 ‐ 10 g in weight) for stock enhancement, and 1, 275, 000 and 3, 000, 000 juveniles (1‐g in weight) for commercial grow‐out. Estimated production costs were lower than for many marine finfish species. The 20‐year mean costs per 1, 000 juveniles were estimated to be $324, $215, and $159, for hatcheries I, II, and III respectively, and the initial capital investments were $731, 660, $1, 162, 460, and $1, 896, 030¹, respectively. Major cost items of annual production were labor (22 ‐ 33%), supplies (14 ‐ 20%), fixed costs (15 ‐ 17%), and interest (13 ‐ 15%). The most profitable hatchery was the 3 million 1‐g juvenile hatchery due to the hatchery size effect, with a net present value (NPV) of $1, 760, 000, an internal rate of return (ERR) of 81%, a payback period of 3.6 years, and a profitability index of $2.86 for every dollar invested. Possible cost reductions to increase financial profitability were discussed.     

Evaluation of Commercial‐scale Approaches for Cryopreservation of White Crappie,Pomoxis annularis,Sperm

Crappie, Pomoxis spp., are popular game fish throughout North America and are produced by public and private hatcheries. However, production is limited by a lack of information on tank culture and induced spawning methods. Development of techniques for storage of sperm and in vitro fertilization would increase flexibility in spawning. Therefore, techniques for sperm cryopreservation were examined in white crappie, Pomoxis annularis. Sperm from adult wild white crappie were used to evaluate sperm extender, cryoprotectant agent and concentration, and cooling technique based on post‐thaw sperm motility. Percent egg fertilization was also compared between sperm stored in the two best cryopreservation protocols and two different osmotic activator solutions. Sperm were cryopreserved using treatment combinations of two extenders (350 mOsmol/kg Hanks' balanced salt solution [HBSS] and 350 mOsmol/kg Ca²⁺free HBSS) and two cryoprotectants (dimethyl sulfoxide [DMSO] and methanol) at concentrations of 5, 10, and 15% that were cooled at four different rates: 5, 10, 20, and 40 C/min. Post‐thaw sperm motility and fertilization rates indicated white crappie sperm can be cryopreserved using either extender, cryoprotectants of either 5% DMSO or 10% methanol, and cooling at 40 C/min. A follow‐up experiment demonstrated sperm in suspensions on ice retained viability after overnight transport.     

Simulation modelling of high‐throughput cryopreservation of aquatic germplasm: a case study of blue catfish sperm processing

Emerging commercial‐level technology for aquatic sperm cryopreservation has not been modelled by computer simulation. Commercially available software (ARENA, Rockwell Automation, Inc. Milwaukee, WI) was applied to simulate high‐throughput sperm cryopreservation of blue catfish (Ictalurus furcatus) based on existing processing capabilities. The goal was to develop a simulation model suitable for production planning and decision making. The objectives were to: (1) predict the maximum output for 8‐h workday; (2) analyse the bottlenecks within the process, and (3) estimate operational costs when run for daily maximum output. High‐throughput cryopreservation was divided into six major steps modelled with time, resources, and logic structures. The modelled production line processed 18 fish and produced 1164 ± 33 (mean ± SD) 0.5‐mL straws containing one billion cryopreserved sperm. Two such production lines could support all hybrid catfish production in the United States and 15 such lines could support the entire channel catfish industry if it were to adopt artificial spawning techniques. Evaluations were made to improve efficiency, such as increasing scale, optimizing resources, and eliminating underutilized equipment. This model can serve as a template for other aquatic species and assist decision making in industrial application of aquatic germplasm in aquaculture, stock enhancement, conservation and biomedical model fish.     

Nonparametric estimation of returns to investment in Honduras shrimp research

Abstract

Economic returns to the investment in shrimp research in Honduras by Auburn University researchers, as a part of the Pond Dynamics/Aquaculture Collaborative Research Support Program (1993 to 1998), were estimated using a nonparametric approach. A survey of shrimp growers in Honduras provided data on yield, input application, and prices for their first year of production and for the year 1997. Research investment data included funding from both public and private sectors. Results showed that total factor productivity indices increased from 1995 to 1997 indicating technical progress due to research. When both private and public investment were considered, the internal rate of return to the investment in research was 46%. However, the internal rate of return to public‐sector investment alone was above 6,681%. This indicated that the public funds invested in shrimp research in Honduras have been leveraged effectively with private‐sector capital to generate technological progress.     

An Analysis of Biological, Economic, and Engineering Factors Affecting the Cost of Fish Production in Recirculating Aquaculture Systems

Aquaculture production in recirculating systems has been the focus of research and development efforts for decades. Although considerable resources have been expended on these systems in the private sector, there is a scarcity of data on the economic or engineering performance of commercial scale recirculating production systems. This paper presents the results of a computer simulation of tilapia production in a small recirculating production system. Much of the performance data has been developed at a demonstration facility at North Carolina State University. Given the assumptions of the base case simulation, the cost of producing a kilogram of tilapia in the recirculating system described is estimated to be $2.79 ($1.27/lb). The results of a model sensitivity analysis indicate that while improvements in the performance efficiency of system components did not greatly affect fish production costs, reductions in feed costs and improvements in the feed conversion ratio caused the greatest reduction of production cost of all of the operational variables investigated. The analysis further indicates that the greatest gains to be realized in improving profitability are those associated with increasing the productive capacity or decreasing the investment cost of a recirculating fish production system.     

THE FARMING PRACTICES AND ECONOMICS OF AQUACULTURE IN THAILAND

ABSTRACT

The paper reviews freshwater and coastal aquaculture practices in Thailand, and compares the productivity, costs, and benefits across various types of cultivation and various intensities of production. The paper is based on data that were collected in surveys conducted during 1998–2001 by the Department of Fisheries (DOF), Thailand and the WorldFish Center. More than 22% of Thailand's fish supply comes from aquaculture, with coastal aquaculture accounting for more than 88% of this in terms of value. Intensive culture of shrimp is the dominant form of coastal aquaculture, occupying 69% of the area under production. However, in some regions, the average net profit/kg of intensive shrimp culture is negative, and semi-intensive farming, with relatively lower fixed investment and operating costs, delivers the highest rate of return on investment. On the coast, grouper and sea bass are the most important cage-cultivated species, achieving an economic rate of return as high as 92%. In the same environment, culture of mollusks, such as green mussels, oysters, and blood cockles, is widespread. It can also be economically sustainable, with relatively low capital and operating costs. Although the relative share of freshwater aquaculture production is declining, the level of output has been increasing rapidly. While the average production from monoculture of carnivorous species is higher than that from polyculture, the average capital investment and operating costs associated with the former are also higher. The expansion of freshwater polyculture and of mollusk culture in coastal areas would greatly assist poor fish farmers.     

The Use of Dairy Protocols for Sperm Cryopreservation of Blue Catfish Ictalurus furcatus

The commercial‐scale production of fish by use of artificial (induced) spawning would require reliable, large‐volume sources of sperm. Cryopreservation can be used to preserve and store sperm within commercial and research germplasm repositories, but is limited in its application to aquaculture. Straw volume and cooling chamber size restrict the quantity of sperm that can be frozen, and straws must be filled by hand. In contrast, the dairy industry has refined methods for freezing of bull sperm, including automation of straw filling and the use of large cooling chambers. These methods could be used for commercial‐scale cryopreservation of fish sperm, although application would require testing. To supply sperm in large volumes, bags originally developed for swine semen could be cooled using dairy protocols and used as a container for fish sperm. The current study documented the use of commercial‐scale dairy cryopreservation techniques for the production of hybrids of channel catfish Ictalurus punctatus (female) by blue catfish Ictalurus furcarus. Four cryoprotectants (methanol, dimethyl sulfoxide, dimethyl acetamide, and glycerol) were initially evaluated for use with blue catfish sperm. During May 2000 and March to April 2001, suspensions of blue catfish sperm were cryopreserved with 10% methanol in 0.5‐mL French straws and in commercial swine semen bags (Cochette^* bags, IMV International. Minneapolis, Minnesota, USA). Cryopreservation took place at a dairy breeding cooperative, using technology employed for bull semen. Sperm motility before freezing was 26 ± 18% during Year 1 (2000) and 62 ± 30% during 2001. Sperm were thawed at 40 C and used to fertilize the eggs of channel catfish (yielding hybrids). Motility after thawing for sperm frozen in 0.5‐mL straws was 11 ± 10% during 2000 and 50 ± 24% during 2001. Motility after thawing was 41 ± 17% for sperm frozen in swine semen bags in 5‐mL aliquots and 43 ± 10% for sperm frozen in 10‐mL aliquots. Neurulation of eggs fertilized with thawed sperm from straws was 83 ± 13% during 2000 and 54 ± 27% during 2001. Neurulation was 57 ± 24% using sperm frozen in swine semen bags in 5‐mL aliquots and 55 ± 10% using sperm frozen in 10‐mL aliquots. There was no correlation between sperm motility before freezing (in 0.5‐mL straws) and after thawing during 2000 (r= 0.52) or during 2001 (r= 0.49). In addition, there was no correlation between initial motility and neurulation of channel catfish eggs fertilized using thawed sperm during 2000 (r= 0.14) or during 2001 (r= 0.29). Sperm of blue catfish can thus be cryopreserved at a commercial scale using dairy protocols and can be made available for the production of hybrid catfish when viable eggs are available.     

Evaluation of the potential source of bacterial contamination during cryopreservation process of silver barb (Barbodes gonionotus) sperm

Application of good sanitation practice in cryopreservation process is the key issue to improve the quality of cryopreserved fish sperm. This study implemented standard sanitation protocol with minimal contamination and evaluated the source of bacterial contamination associated with laboratory equipment and related materials across a series of cryopreservation process of silver barb (Barbodes gonionotus) semen. The use of 16s rRNA sequencing and traditional biochemical methods were performed for bacterial identification. Animal origin (anal fin, culture water and semen contaminated with faeces and urine) and non‐animal origin (liquid nitrogen from liquid nitrogen dewar, outer surface of straw, air circulation in cryopreservation laboratory and latex gloves used during cryopreservation procedure) were determined for bacterial contamination. Aeromonas punctata subsp. caviae was the most abundant species in anal fin, latex groves and semen contaminated with faeces and urine. Bacillus safensis and Bacillus sp. were found as frequently recovered species from liquid nitrogen dewar. Aeromonas hydrophila subsp. hydrophila and Pseudomonas fluorescens, fish pathogenic bacteria, were isolated from all animal origin samples. This was the first report indicating that standard sanitation and hygiene methodologies are recommended for sperm cryopreservation of B. gonionotus to prevent bacterial contamination.     

Economic feasibility of recirculating aquaculture systems in pangasius farming

This study aims to analyze the economic feasibility of recirculating aquaculture systems (RAS) in pangasius farming in Vietnam. The study uses a capital budgeting approach and accounts for uncertainty in key parameters. Stochastic simulation is used to simulate the economic performance of medium and large farms operating with a traditional system or RAS. Data are obtained through structured surveys and a workshop in the Mekong River Delta. Results show that for large farms, net present value increases from an average of 589,000 USD/ha to 916,000 USD/ha after implementing RAS. Overall, the probability that RAS is a profitable investment is found to be 99% for both farm sizes. With RAS, the crucial parameters determining profitability are price, yield, costs of fingerling, feed, and initial investment. Findings on the robustness of the economic performance of RAS are useful to support public and private decision making towards increasing the sustainability of pangasius production.     

ECONOMIC FEASIBILITY OF SMALL-SCALE BLACK-LIPPED PEARL OYSTER (PINCTADA MARGARETIFERA) PEARL FARMING IN THE CENTRAL PACIFIC

ABSTRACT

This work provides an analysis of the economic feasibility of one of many small-scale aquaculture operations being considered, black pearl oyster farms, as one type of supplemental economic activity for outer island communities in the Central Pacific. Specifically, projections of financial performance of a small-scale 25,000 seeded pearl oyster farm using the Tahitian long-line method are being conducted. Estimates of initial capital investment and annual operating costs are being formulated, an annual cash flow and enterprise budget are being developed. Results show that initial capital investment is $202,076. Annual operating expenses are $293,726 during full operation. The largest costs contributing to annual operating expenses are seeding (46%), labor including farm owner's opportunity cost (24%), and depreciation (9%). The base model presented in this work suggests profitability over a 20-year horizon.

Net returns over a 20-year farm horizon based on an 8% discount rate indicate a positive NPV of $ 102945. Sensitivity analysis on profit due to the variability of market price, survival, and cost of seed and other inputs are conducted and results presented.     

Potential of New Strains of Marine and Inland Saline-Adapted Microalgae for Aquaculture

Several problems currently limit the reliability of algal cultivation systems for the production of aquaculture feeds. Many of these problems may be eventually solved through research in culture system design and operation. However, research into the isolation and development of new strains of microalgae may also directly lead to improved system performance.
Through a collection and screening program aimed at isolating microalgae tolerant to high light, high salinity, and high temperatures, we have been able to isolate several new strains with enhanced production potential (30-35 g/m²/d). The overall range of environmental tolerance exhibited by these strains may result in enhanced culture stability, leading to the high production rates.
The estimated costs of producing strains with these high production rates range from $1.18 to S1.71 per kg (unharvested), depending on the size of the production system. Total production costs for harvested algae (15% solids algal cell paste) range from $1.63 to $2.45 per kg. The major costs for producing harvested algae include the cost of CO₂, as well as capital and operating costs for the harvesting systems.     

Farm-level costs of settling basins for treatment of effluents from levee-style catfish ponds

Compelled by pending regulatory rule changes, settling basins have been proposed as a treatment alternative for catfish pond effluents, but the associated costs to catfish farmers have not been estimated. Economic engineering techniques were used to design 160 scenarios as a basis for estimating total investment and total annual costs. For static-water, levee-style catfish pond facilities, sizing of settling basins is controlled by factors such as type of effluent to be treated, pond layout, size of the largest foodfish pond, number of drainage directions, scope of regulations governing effluents, and the availability of land. Regulations that require settling basins on catfish farms would increase total investment cost on catfish farms by $126–2990 ha⁻¹ and total annual per-ha costs by $19–367 ha⁻¹. More numerous drainage directions on farms resulted in the greatest increase in costs. While both investment and operating costs increased with larger sizes of foodfish ponds, costs per ha were relatively greater on smaller than on larger farms. For farms on which existing fish ponds would have to be converted to settling basins, over half of the cost was due to the production foregone and annual fixed costs of the pond. Requiring catfish farmers to construct settling basins would impose a disproportionately greater financial burden on smaller farms. The magnitude of the increased costs associated with settling basins was too high relative to market prices of catfish for this technology to be economically feasible.     

Economic Evaluation of a Small-Scale Recirculating System for Ongrowing of Captive Wild Black Sea Bass Centropristis striata in North Carolina

An economic analysis of a hypothetical small‐scale marine recirculating aquaculture system (RAS) is conducted for ongrowing small, wild black sea bass Centropristis striata at the University of North Carolina Wilmington, Center for Marine Science (UNCW‐CMS) aquaculture facility in Wrightsville Beach, North Carolina (NC). The analysis is based on production data from field trials and marketing data from the sale of tank‐grown product. The growout facility consists of four 16.7‐m³ (dia. x ht. = 5.58 × 1 m) fiberglass tanks supported by state‐of‐the‐art RAS components, including particle traps and swirl separators, drum screen filter, trickling biological filter, UV sterilizer, heat pump, protein skimmer, and oxygen cone. Wild‐caught, above minimum legal size black sea bass (24.2 cm TL, 350 g, 0.77 lb) were purchased from a commercial fisherman for $3.14/ kg ($1.4011b), stocked at a density of 21.1 kg/m³, and grown to a final weight of 1 kg (2.24 lb) in 200 d at 23 C resulting in 1.8 production cycles per year. Fish were fed a commercial pelleted diet ($0.94/kg; $0.42/Ib) with a feed conversion ratio of 1.5. Final harvest density was 60 kg/m³ (0.50 lb/gal), and total harvestable weight was 3,982 kg (8,919 Ibs) of fish per cycle, or 6,760 kg (15,022 lb) per year. The economic analysis assumes that the facility owner manages and operates the system on coastal property zoned commercial/industrial, where full strength seawater is available on demand from natural sources. Under the base case scenario, initial investment in construction and equipment is $84,506 (10‐yr life), fish are grown to a harvestable weight of lkg/fish (2.24 lb/fish), product price (farm gate basis) is $10.10/kg ($4.50/lb), and breakeven price is $7.02/kg ($3.13/lb). Depreciation, fingerlings, interest paid, electricity, and feed, account for 19.6%,17.4%, 16.9%, 16.6%, and 12.3%, respectively, of total annual costs. Measures of financial performance for the base case, 10‐yr scenario are: annual return to management, $18,819; net present value (5% discount rate), $145,313; internal rate of return on initial investment, 37%; and discounted payback period on initial investment, 3.2 yr. Sensitivity analysis showed that product price changes have the largest impact on annual returns, while changes in daily growth rate, initial weight, and survival have a strong impact on financial performance. Moderate effects are seen with changes in fingerling costs, feed costs, feed conversion ratio (FCR), final weight, and interest rates.     

Cryopreservation of sperm from spotted wolffish

The effects of different concentrations of cryoprotectant (dimethyl sulfoxide; DMSO), cooling rate and straw size on the post-thaw motility of frozen sperm from spotted wolffish, Anarhichas minor, were studied. There was no significant difference in the post-thaw motility of sperm treated with three different concentrations of DMSO (10, 20 and 30%). Similarly, there was no significant difference in the post-thaw motility of spermatozoa when using different freezing rates (i.e. distance of straws from the surface of liquid N₂, 4.7, 5.5 and 7.1°C min⁻¹) and the straw size (0.5 and 1.0 ml) did not affect survival. The cryopreservation of sperm can be used to make up for the frequent lack of sperm and/or the unsynchronised timing of sperm production in spotted wolffish males and the ovulation time in females. The results show that sperm from spotted wolffish can be frozen to secure access to viable sperm, but further experiments are needed in order to reveal the effect of different parameters on the post-thawing mortality and define the optimum conditions for cryopreservation.     

Challenges and emerging technical solutions in on-growing salmon farming

Farming of Atlantic salmon has grown rapidly from its start in the early 1970s until today, with production approaching two million tonnes. Sea cages are the dominant production system for the on-growing stage of salmon farming. It represents an effective production system with lower investment and running costs than land-based systems. The development and improvement of the sea cage farming system has been one of the most important factors for the growth of the salmon farming industry. However, during recent years certain problems related to their placement in the open marine environment have proved highly challenging, increasing operating costs and impacting on industry public relations. The problems are mainly due to parasites, diseases and escape of fish. In this article, emerging technical solutions for solving those problems are described.     

Sperm quality and cryopreservation of Brazilian freshwater fish species: a review

The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as piracema). Urbanization, pollution, hydroelectric dams and deforestation are some of the causes of stock depletion or even local extinction of some of these species. The knowledge concerning sperm quality and minimum sperm:egg ratio is important to maximize the use of males without reducing hatching rates. Furthermore, sperm cryopreservation and gene banking can guarantee the conservation of genetic diversity and development of adequate breeding programs of native fish species. In this review, we present and evaluate the existing information on Brazilian fish species that have been subject to sperm quality and cryopreservation studies. The following parameters were evaluated: volume of extractable sperm, sperm motility, sperm concentration, freezing media, freezing methods, and post-thaw sperm quality. Although the existing protocols yield relatively high post-thaw motility and fertilization rates, the use of cryopreserved sperm in routine hatchery production is still limited in Brazil.     

Cryopreservation of turbot Scophthalmus maximus (L.) sperm: fertilization and hatching rates

The present paper assesses the fertilization and hatching rates of an artificial fertilization series (n=1153) using fresh and cryopreserved sperm from 49 specimens of turbot Scophthalmus maximus (L.), carried out to confirm the results of a previous study, with the ultimate aim of transferring these cryo‐preservation techniques to commercial hatcheries. No significant differences were found between the fertilization rates of the two groups (fresh and cryopreserved sperm) when their respective fertility rates were >69.2%, which was the case in 75% of all fertilizations. Likewise, no significant differences in hatching rates were found. In order to use the cryopreserved sperm more efficiently, a key concern for commercial use of this technique, we also experimented with a lower sperm:diluent ratio (1:1) than used previously at our centre. We also compared the traditional 0.5‐mL straws with 2‐mL cryotubes able to contain a higher volume of sperm, finding no significant differences in the resulting fertilization and hatching rates. In conclusion, the use of cryopreservation for turbot sperm presents major advantages for broodstock management in commercial hatcheries.