The influence of age and gender on haematological parameters in Lipizzan horses

Haematological parameters [red blood cell count (RBC), white blood cell count (WBC), packed cell volume (PCV), haemoglobin concentration, mean cell volume (MCV), mean cell haemoglobin (MCH) and mean cell haemoglobin concentration (MCHC)] in resting Lipizzan horses were determined for 143 stallions, 104 mares and 25 foals. The mean RBC and WBC values in Lipizzans were in the lower part of the normal range for warm-blooded horses. The mean PCV, MCV and MCH values were higher, but the mean haemoglobin concentration and MCHC values were lower than reported for other warm-blooded horses. In foals, the mean RBC, WBC, PCV, haemoglobin concentration and MCHC values were higher, whereas MCV and MCH were lower than in older animals. Results indicating a significant decrease in WBC (P < 0.01) and an increase in MCV, MCH and MCHC (P < 0.05) with increasing age are consistent with some other reports on warm-blooded horses. The age-related variations in RBC and PCV were less marked. Contrary to some reports, RBC (P < 0.01), WBC and haemoglobin concentration (P < 0.001) were higher in Lipizzan stallions than in mares, but differences in MCHC, MCH and MCV were insignificant. The specific haematological values determined in Lipizzans are presumably a result of selection and should be taken into consideration when dealing with this race of horses.     

Haematological responses of repeated large volume blood collection in the horse

The haematological response of regular, repeated blood harvesting was investigated in 40 Thoroughbred and non-Thoroughbred horses that donate 8 litres of blood every 3 weeks for the purposes of commercial blood production. When this volume of blood was removed on five occasions over 12 weeks, no adverse effect on packed cell volume (PCV), haemoglobin (HB), and red blood cell count (RCC) was observed. Although PCV, RCC and Hb values decreased during the first week after blood collection, followed by a gradual increase in values until the next harvest time, all values remained within published reference ranges. Derived red cell indices [mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin concentration (MCHC)] also remained within reference range. We conclude that the removal of approximately 8 litres of blood (approximately 16 ml kg(-1)or 20 per cent of blood volume for a 500 kg horse) from blood donor horses every 3 weeks allows time for adequate recovery of haematological variables and does not result in adverse haematological changes.     

果寡糖对固始鸡血液生理指标的影响

本试验旨在研究不同添加剂量的果寡糖对固始鸡血液生理指标的影响,并确定果寡糖的最佳添加剂量。选用1日龄固始鸡公雏150只,随机分成5个处理组,1个为对照组,其余4个为试验组,对照组不加果寡糖,其他4个组的果寡糖添加水平分别为3、5、7mg/kg和9mg/kg。测定红细胞压积(PCV)、血红蛋白(Hb)含量、红细胞数量、白细胞数量、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)和平均红细胞血红蛋白浓度(MCHC)等指标。试验结果表明:1)21和42日龄时,果寡糖试验组的红细胞压积、血红蛋白含量、红细胞数量和白细胞数量均显著高于对照组(P<0.05);与对照组相比,试验组的MCH和MCHC均略有升高,并且可以显著提高MCHC(P<0.05);2)日粮中添加5mg/kg果寡糖对提高红细胞压积、血红蛋白含量、红细胞数量、白细胞数量、MCH和MCHC具有最佳效果。     

Haematological profile of crossbred dairy cattle to monitor herd health status at medium elevation in Central Himalayas

Haematological profile-haemoglobin concentration (Hb), total erythrocytes count (TEC), packed cell volume (PCV), erythrocyte indices-mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were studied in crossbred dairy cattle (Holstein Friesian x Sahiwal) under various physiological states: non-pregnant heifers (NPH), pregnant heifers (PH), empty dry cows (EDC), pregnant lactating cows (PLC), medium yield early lactating cows (MYELC) and high yield early lactating cows (HYELC) during summer and winter seasons at 1700 metres altitude from mean sea level in the Central Himalayas. On comparison of annual means, the highest values of Hb and PCV were recorded in PH and of TEC in NPH, whereas the lowest values of these parameters were found in EDC. The Hb and TEC tended to decrease with increasing milk yield. Comparison of annual means of erythrocyte indices revealed the highest MCV and MCH in EDC, which simultaneously showed the lowest MCHC. Significant seasonal variations in haematological profile were recorded. The overall group mean (OGM) of Hb, MCV, MCH and MCHC was found to be significantly higher (P < 0.01) during summer whereas the TEC and PCV showed higher OGM (P < 0.01) during the winter season.     

The haematological examination of canine blood samples received by post: the influence of delay in examination on red cell parameters

The mean packed cell volume (PCV) for canine blood samples received by post was significantly higher than that for fresh samples, at each haemoglobin value over the range 9 to 19 g/dl. As a consequence the mean corpuscular volume (MCV) and the mean corpuscular haemoglobin concentration (MCHC) also differed. Apart from one Hb value there was no significant difference in the mean red blood cell (rbc) count over the Hb range 6 to 14 g/dl. It is suggested that the normal values for the PCV and MCV should be 3 to 5% and 5 to 7 f 1 higher respectively for postal samples as compared to fresh samples while the MCHC should be about 3 to 4 g/dl lower.     

家兔在西藏海拔3000m条件下部分生理特性的试验观察

试验对海拔 3 0 0 0m的藏南谷地林芝引入家兔进行了部分生理指标的对比分析。测定了呼吸频率、心率、生理体温、血清蛋白质含量和血红蛋白含量。统计分析表明 :所测定项目的结果在 3品种兔之间均无明显差异 (P >0 0 5) ;但血红蛋白电泳分析 :3品种兔均呈现出A、A2 ,A33个区带 ,发现A3带以青紫兰兔最低 ,与其他 2品种兔差异极显著 (P <0 0 1 ) ;加里福尼亚兔A3带最高 ,显著高于中国白兔 (P <0 0 5)及青紫兰兔 (P <0 0 1 )。血清蛋白质电泳分析表明 :高原家兔的血清白蛋白均明显高于文献报道的低海拔饲养的新西兰白兔 ,血清总蛋白及其球蛋白含量明显下降     

Studies on some paraclinical indices on intoxication in horses from freshly cut Jimson weed (Datura stramonium)-contaminated maize intended for ensiling

Monitoring of changes in some blood laboratory parameters in 34 horses after ingesting freshly harvested maize that was to be used for ensiling, heavily contaminated with young Datura stramonium plants, is described. For a 7-day period the following parameters were monitored: haemoglobin content (HGB), red blood cell counts (RBC), white blood cell counts (WBC), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), differential white cell counts (DWC), erythrocyte sedimentation rate (ESR), protein fractions, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total bilirubin (TB), direct bilirubin (DB), blood glucose (Glu), total protein (TP), globulin (Glob) and albumin (Alb). The intoxication was accompanied by erythrocytosis, leukocytosis, regenerative left shift neutrophilia, lymphopaenia, eosinopaenia, increased haematocrit values, low erythrocyte sedimentation rate, hyperglycaemia, bilirubinaemia, hypoproteinaemia and increased activity of AST and LDH. No changes occurred in the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), albumin, globulin and globulin fractions (alpha 1, alpha 2, beta 1, beta 2 and gamma). The blood parameters returned to normal between post-intoxication days 2 and 5. The observed changes in clinical chemistry indices could be used in the diagnosis, differential diagnosis and prognosis of Jimson weed intoxication.     

The haematological and biochemical effects of the gastrointestinal nematodes prevalent in camels (Camelus dromedarius) in central Saudi Arabia

Coprological examinations on 1200 Najdi camels (Camelus dromedarius) in the Gassim region of central Saudi Arabia between October 1992 and September 1993 revealed the presence of strongyletype, Nematodirus and Strongyloides eggs. In addition, examination of the gastrointestinal tracts of 240 camels slaughtered in different abattoirs in the Gassim region from March to August 1994 revealed 11 species of helminths. Infection with gastrointestinal trichostronglyes resulted in normocytic, normochromic anaemia. Adult infected camels showed leukocytosis, a decrease in total serum protein concentration and increases in blood urea concentration and in serum creatine phosphokinase activity.Abbreviations ALP alkaline phosphatase - AST aspartate aminotransferase - CPK creatine phosphokinase; epg, eggs per gram - GGT gamma-glutamyl transferase - Hb haemoglobin - MCV mean corpuscular volume - MCHC mean corpuscular haemoglobin concentration - PCV packed cell volume - RBC red blood cell     

Haematological changes in domestic fowl experimentally infected with the cestode Raillietina tetragona (Molin, 1858)

Haematological alterations in domestic fowl following three levels of infection with 20, 50 and 100 cysticercoids of Raillietina tetragona (Eucestoda) have been studied over a period of 8 weeks. The infected birds showed significant haemoglobin depression. There was no significant change in the packed cell volume (PCV), RBC counts or mean corpuscular haemoglobin (MCH). In all three levels of infection, the calculated mean corpuscular volume (MCV) increased, while the mean corpuscular haemoglobin concentration (MCHC) decreased considerably. Macrocytic anaemia along with hypochromia could be noticed during different periods of infection. Total white cell count diminished significantly in infected birds. There was a significant lymphocytopenia concurrent with a significant increase in the percentage of heterophilic elements. Eosinophilia was not a consistent feature. Erythrocyte sedimentation rate (ESR) increased significantly in infected birds. The changes in the blood picture were more pronounced in birds infected with 50 and 100 cysticercoids.     

Erythrocyte changes, serum iron concentration and performance following iron injection in neonatal beef calves

The injection of iron-dextran into beef calves at an early age immediately increased values for erythrocyte (RBC) numbers, hemoglobin (Hb) concentration, packed cell volume (PCV), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV), even though the calves were not anemic. There were no significant differences in average daily gain (ADG) from birth through 15 wk between those that were injected and those that were not injected. Hereford calves had lower values for PCV, Hb, MCH and MCV than Angus and crossbred calves, and both Hereford and Angus calves had lesser body weight and slower ADG than crossbred calves. Serum iron (Fe) concentrations were higher in calves born in the spring and pastured in the summer than in calves born in late summer and fall and pastured in the fall. The serum Fe concentration of calves at first sampling (during first week of life) was positively correlated with their weight at that and subsequent times to 15 wk. Mean corpuscular volume and MCH in cows before calving were positively correlated with the MCV and MCH in their calves at first sampling. Serum Fe concentration in cows before calving was negatively correlated with the ADG of their calves through 15 wk.     

Effect of gender and exercise on haematological and biochemical parameters in Holsteiner horses

The objective of this study was to assess the influence of exercise of average intensity in the haematological and biochemical values, as well as acidic resistance of erythrocytes in mares and stallions of Holsteiner breed. A total of seventeen horses of Holstein breed (seven mares and 10 stallions aged 6 years) were used in this study. The blood samples were assessed for haematocrit (HCT) value, haemoglobin concentration (HGB), the amount of red blood cells (RBC), white blood cells (WBC), platelets (PLT), leucogram, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), red cell distribution width (RDW) and platelet distribution width (PDW). Serum concentrations of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) as well as oxidative stress biomarkers were analysed. Stallions showed a significant increase in leucocytes and granulocytes amount, as well as erythrocytes, haemoglobin and haematocrit levels after exercise test. Pre‐exercise level of mean corpuscular haemoglobin concentration was higher in stallions. At the same time, mares showed significant decrease in platelet volume after exercise test. Physical effort in stallions leads to significant increase in aspartate aminotransferase activity. Trained mares and stallions showed a decrease in lipid peroxidation after exercise. Exercise also caused increase in oxidative modified protein of erythrocytes in stallions indicating by exercise‐induced oxidative stress. The resistance of erythrocytes in 0.1 m HCl was similar between females and males. No statistically significant differences in the percentage of haemolysed erythrocytes before and after exercise were observed.     

Studies on the relationship between sway disease of bactrian camels and copper status in Gansu province

The clinical signs of camel sway disease in the Hexi Corridor of Gansu province were defined. The contents of eight minerals in soils, in forage and in the blood and hair of bactrian camels from this region were determined. The related blood indices were also measured. The concentration of molybdenum in soils and forage was 4.8±0.02 and 4.8±0.25 µg/g (dry matter), respectively, the copper to molybdenum ratio in the forage being only 1.3. The concentration of copper in blood and hair from the camels was 0.28±0.17 µg/ml and 3.50±1.00 µg/g, respectively. There was a hypochromic microcytic anaemia and a low level of ceruloplasmin in the blood. It is therefore suggested that sway disease of bactrian camels in this region is caused by secondary copper deficiency, mainly due to the high molybdenum content in soils and forage. The copper deficiency in the camels was aggravated during reproduction. Oral administration of copper sulphate can prevent and cure the disease.Abbreviations AKP alkaline phosphatase - BUN blood urea nitrogen - Chol cholesterol - Cp ceruloplasmin - Crt creatinine - DM dry matter - -GGT -glutamyltransferase - Hb haemoglobin concentration - LDH lactate dehydrogenase - MCH mean corpuscular haemoglobin content - MCHC mean corpuscular haemoglobin concentration - MCV mean corpuscular volume - PCV packed cell volume - RBC red blood cell count     

Hematology in sika deer (Cervus nippon yesoensis Heude, 1884)

Blood samples were taken from 78 wild and 21 farmed sika deer (Cervus nippon yesoensis) using ketamine-xylazine sedation during their excited (82 deer) and resting (17 deer) states. Red cell count (RBC), hemoglobin (Hb), packed cell volume (PCV) and mean corpuscular volume (MCV) were significantly higher and mean corpuscular hemoglobin concentration (MCHC) was lower in excited deer than in resting deer. There was no significant difference in total leukocyte count (WBC) between excited and resting wild males, while a marked increase of WBC with neutrophilia was observed in excited wild females. RBC and PCV were significantly higher and MCH was lower in excited males than in excited females. In wild deer, WBC was significantly higher in females than in males, but there was no significant difference in WBC between farmed males and females. Sex differences in the hematological parameters were not observed in fawns (10 months).     

Change in Some Physiologic Variables Induced by Italian Traditional Conditioning in Standardbred Yearling

The goal of this study was to quantify the effect of a traditional Italian conditioning method (CM). Six Standardbred yearlings were kept in standardized conditions for 140 days. From T1 (day 1) to T10 (day 10), the horses were hitched for 30 minutes/day to accustom them to a cart. From T11 to T70, they were jogged or exercised for 30 minutes/day at a slow trot (4.4 ± 0.3 m/second) and from T71 to T140 for 40 minutes/day (5 m/second ± 0.3 m/second). Every 15 days, the horses performed fast exercise for a distance of 5,000 m with increased speeds. Blood samples were collected before and after daily training (DT) every 21 days (P1…P7), to measure the following variables: erythrocytes (RBC), hemoglobin (Hb), packed cell volume (PCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), total protein (Pt), albumin, aspartate amino transferase (AST), alkaline phosphatase (ALP), creatine kinase (CK), lactate dehydrogenase (LDH), urea, creatinine, glucose, triglycerides, and blood lactate. A three-step standardized exercise test (SET) also was performed. Analysis of variance was used to study differences during the training. Tukey's post-hoc test was used for statistical multiple comparison (P ≤ 0.05 and 0.01). Lactate level did not show an increase over 4 mmol/L in all periods. Glucose was higher at P1 than P7. There was no correlation between method of conditioning and the level of CK, LDH, or AST. Conditioning method induced an increase in triglycerides for the change in exercise metabolism supply. According to SET, conditioning method induced an increase of SVO2_max (speed [m/second] reached to a theoretical maximal oxygen consumption), V₄ (speed [m/second] reached at a lactate content of 4 mmol/L), V₂₀₀ (speed [m/second] at 200 beats/minute) and HR₄ (heart rate [beats/minute] at 4 mmol/L of lactate). We concluded that the Italian conditioning method used in this study improved the fitness of horses.     

Haematological values of post-laying Arrau turtle (Podocnemis expansa) in the Orinoco River, Venezuela

The Arrau turtle (Podocnemis expansa) is an endangered species, as a result of long-lasting, unsustainable exploitation. To obtain reference haematological values from the wild Podocnemis expansa during post-laying, 20 turtles were captured in the Orinoco River. Blood was obtained from the dorsal cervical sinus in lithium heparin tubes. Red blood cells (RBC), white blood cells (WBC), thrombocytes (TC), packed cell volume (PCV), plasmatic protein (PP), haemoglobin (Hgb), mean corpuscular volume (MCV) and differential leukocyte count were determined. Haematological values were: RBC 0.9×10(9)/L, WBC 5.7×10(9)/L, TC 5.4×10(9)/L, PCV 35.6%, PP 4.2g/dL, Hgb 11.8g/dL, MCV 411fL. The differential leukocyte count comprised: 71% heterophils, 23% lymphocytes, 3% eosinophils, 1.6% basophils, and 1% monocytes. The reports of reference haematology values for the wild P. expansa are limited; therefore, the results presented herein contrast with those values obtained in captivity. This study represents a contribution to the referential haematological values of the wild P. expansa.     

Antioxidant and haematological biomarkers in different groups of horses supplemented with polyunsaturated oil and vitamin E

Oxidative stress has been correlated with pathologies that impair the performance of athlete horses. The aim of this study was to assess the effects of supplementation with a mixture of polyunsaturated oil and vitamin E on the antioxidant and haematological biomarkers of horses. Horses under maintenance care (n = 6) and horses in training (n = 10) received 100 and 300 ml of the oil mixture respectively. Supplementation was provided for a period of 8 weeks, together with isocaloric inclusion. Blood samples were collected at three time periods (pretest, after 4 weeks and after 8 weeks) to analyse the following: the red blood cell count (RBCc); haemoglobin (Hb); haematocrit (HT); leucocytes; lymphocytes; platelets; the mean corpuscular volume (MCV); the mean corpuscular haemoglobin concentration (MCHC); the standard deviation of the red blood cell distribution width (RDW‐SD); the coefficient of variation of the red blood cell distribution width (RDW‐CV); glutathione peroxidase (GPx); superoxide dismutase (SOD); uric acid (UrAc); total plasma proteins (TPP); and creatine kinase (CK). After the 8 weeks of supplementation, animals under maintenance care exhibited significant increases in SOD, UrAc, the white blood cell count (WBCc), the RDW‐SD and the RDW‐CV (p < 0.05). The animals in training exhibited increases in GPx, SOD and UrAc (p < 0.05). In conclusion, supplementation with polyunsaturated oil and vitamin E increases blood antioxidants among animals under maintenance and in training, with different trends, while contributing to the fight against oxidative stress in each group analysed.     

HAEMATOLOGY OF THE RACEHORSE: A Recent Study of Thoroughbreds in Victoria

ab>The study was based on 216 blood samples collected from 136 Thoroughbred racehorses in 17 stables. Lower variability in the population studied and lower means than in previous studies were attributed in part to measures taken to reduce excitement of the horses during blood collection. The demeanour of the animals during collection was assessed subjectively as placid, timid, apprehensive or excited. The five horses which became excited had significantly higher RBC, Hb, PCV, WBC, total eosinophil count, serum total protein and probably lower ESR. Timid and apprehensive horses did not show these responses. Because of the location of eight stables, samples from them were stored overnight at 4°C before analysis. These samples had higher mean MCV, PCV, MCH, and serum total protein and lower mean MCHC. The likelihood of this arising from swelling and perhaps rupture of some erythrocytes during transportation and storage was discussed. Serum total protein estimates were within the range determined from other reports from horses of various breeds. However albumin and the A/G ratio determined by electrophoresis were higher, but comparable with two other reports from Thoroughbreds. In comparative studies on the estimation of A/G ratios it appeared that equine serum albumin determination was higher using the bromcresol green reagent than by electrophoresis. After eliminating 14 cases with abnormalities of the total and/or differential leucocyte count, parameters were calculated from 202 samples for the definition of normal ranges. The absence of skewness and kurtosis indicated that the individual estimates of RBC, Hb, PCV, WBC, MCV and MCH followed the normal distribution and that the means and standard deviations were appropriate for defining normal ranges. With only ESR and TEC were the coefficients of variation, skewness and kurtosis so great as to cast doubt on the practical use of these estimates.     

Erythrocytic oxidative damage in crossbred cattle naturally infected with Babesia bigemina

This study aimed to determine the erythrocytic lipid peroxidation and haemoglobin oxidation as contributory factors causing anaemia in cattle (Friesian × Egyptian native breed) infected with Babesia bigemina. Blood was collected from 32 cows infected with B. bigemina along with 18 healthy cows as controls for determination of erythrocytic malondialdehyde (MDA), blood methaemoglobin (MetHb), plasma free haemoglobin (PHb), corpuscular osmotic fragility (COF), red blood cell count (RBC), total haemoglobin (Hb) and packed cell volume (PCV). Percentage of parasitaemia varied from 14% to 36%. MDA, MetHb, COF and PHb were significantly increased (P < 0.001) in infected cows versus controls. Parasitaemia was positively correlated (P < 0.001) with MDA, MetHb, COF and PHb. MDA was positively correlated (P < 0.001) with COF and PHb and negatively correlated (P < 0.001) with RBC, Hb and PCV. MetHb was negatively correlated (P < 0.001) with RBC, Hb and PCV and positively correlated (P < 0.001) with COF. In conclusion, B. bigemina infection in cattle is associated with a parasitic burden-dependent corpuscular oxidative damage as indicated by membrane lipid peroxidation and methaemoglobin formation, which are contributed to COF and intravascular haemolysis.     

Effect of different dietary levels of Yucca schidigera powder on the performance,blood parameters and egg yolk cholesterol of laying quails

A total of 135 laying quails (Coturnix coturnix japonica), 9 weeks old, were divided into three dietary treatment groups. Three replicates were assigned to each treatment group consisting of 15 birds per cage. The diet was supplemented with 0, 100 and 200 ppm Yucca schidigera powder and given ad libitum to the quails for a period of 14 weeks. Body weight, egg production, feed consumption and feed efficiency were not different due to dietary treatments among the groups. Increased egg weight was determined in the control group. Yucca powder supplementation decreased serum glucose, cholesterol triglyceride level in laying quails. Serum total protein concentration was not changed by dietary treatments but albumin level was decreased in quails fed 100 ppm yucca powder. Egg yolk cholesterol concentration was not significantly different among the groups but tended to decline (11.5%) as a result of yucca supplementation. Red Blood Cell (RBC) and White Blood Cell (WBC) counts, packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) were not affected by supplementation of yucca powder. However, haemoglobin (HB) concentration was slightly increased and mean corpuscular haemoglobin concentration (MCHC) was significantly increased by 200 ppm yucca powder supplementation to the diet.     

Disturbance of oxidant/antioxidant equilibrium in horses naturally infected with Trypanosoma evansi

Oxidant/antioxidant equilibrium disturbance has already been reported in trypanosome infections by several authors. The present study was aimed to explore the possible oxidant/antioxidant disturbance in surra of naturally infected horses before and after treatment. Fifteen naturally infected horses were chosen to analyse erythrocytic indices, platelet counts, lipid peroxides (LPO), nitric oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) while six healthy animals acted as control. There was a highly significant (P<0.001) reduction in red blood cell (RBC) count, haemoglobin (Hb), packed cell volume (PCV) and platelet levels and a significant reduction in mean corpuscular haemoglobin concentration (MCHC) (P<0.05) was noticed. A highly significant increase in NO (P<0.001), a significant increase in LPO (P<0.05) and a significant decrease in GSH, SOD and CAT (P<0.05) were found. A negative correlation of RBC count with LPO (r=-0.844) and nitrate (r=-0.702) while a positive correlation with GSH (r=0.489), SOD (r=0.580) and CAT (r=0.689) was observed. All the animals were treated with Quinapyramine sulphate (3mg/kg s.c.) only once. Nine animals recovered completely without any side effects. The recovered animals were monitored and samples were collected every seven days for up to 21 days and parameters were analysed. After treatment, a significant increase in haematological parameters was noticed whereas the oxidative indices varied without any statistical significance. To conclude, the increase in oxidant parameters and decrease in antioxidant enzymes in infected horses indicates the disturbance of oxidant/antioxidant indices. There was a significant increase in post therapy haematological values, while the oxidant/antioxidant indices changed insignificantly indicating that antioxidants might be supplemented in the therapeutic regimen.