Thymus zygis subsp. Gracilis: watering level effect on phytomass production and essential oil quality

Thymus zygis subsp. gracilis (chemotype thymol) was evaluated on the basis of its phytomass production and essential oil quality. Three different watering levels were assayed to achieve 63, 44, and 30% of the local potential evapotranspiration (Eto). According to the statistical analysis, a water supplement equivalent to 44% Eto in this cultivation area was optimal for maximum plant dry matter production and essential oil yield. Capillary GC-MS analysis of the essential oil allowed the identification of 86 volatile components. Among them, 30 are described for the first time as volatile constituents of the essential oil in this thyme subspecies and chemotype. The watering level effect on essential oil composition was noticeable, because the application of a water supplement equivalent to the 63% Eto favored the production of an essential oil richer in low molecular weight components. However, the greatest thymol concentrations were obtained under the 30 and 44% Eto watering levels.     

Responses of Anise Medicinal Plant Species in Terms of Essential Oil Contents and Concentrations to Different Planting Times and Various Nitrogen Fertilizer Sources under Semi-Arid Climatic Conditions

Essential oil content and concentration of anise plant planted in different times under various sources of nitrogen was evaluated in this study. The experiment was conducted in a split plot based on a randomized complete block (RCB) design (different sources of fertilizer as the main plot and planting dates as subplots) with three replications in the Agricultural Research Station at Ferdowsi University of Mashhad in 2015–2016. Treatments included: planting times (sub plot factor): (27^th of October, 27^th of November, 1^st of March, 10^th of March, and 8^th of April) and nutritional sources (main plot factor): (Cow Manure, Chemical Fertilizer, and mixture of Cow Manure + Chemical Fertilizer). The results showed that the anise plant essential oil qualitative characteristics were significantly influenced by treatments. Also, interaction between the fertilizer treatments and planting dates on the essential oil content and concentration of the plants were significant. The highest percentage of plant essential oil (3.71%) was found in the cow manure treatment, and the lowest percentage of essential oil (2.95%) was obtained in the chemical fertilizer. The highest essential oil yield (16.09 kg/ha) was found in the mixed treatment of the cow manure and the chemical fertilizer, and the chemical fertilizer treatment had the lowest rate of essential oil yield (11.41 kg/ha). The highest percentages of the plant oil (14.31%) and the plant oil yield (77.46 kg/ha) were found in the integrated treatment of the cow manure and the chemical fertilizers. The lowest amounts of the plant oil (12.19%) and the plant oil yield (41.28 kg/ha) were found in the chemical fertilizer. According to the percentage and the yield of the essential oil, the best planting time and fertilizer treatment was the one on 10th of March and using integrated cow manure and chemical fertilizer.     

Effect of Vermicompost and Chemical Fertilizer on Growth,Herb, Oil Yield,Nutrient Uptake,Soil Fertility,and Oil Quality of Rosemary

Essential oil of rosemary (Rosmarinus officinalis L.) possesses good olfactory properties and is suitable for use in perfumes, soaps, and fragrances. Field experiments were conducted for 2 years (2003?2005) in an area experiencing a semi-arid tropical climate to study the influence of vermicompost and chemical fertilizer on growth, herb, oil yield, nutrient uptake, soil fertility, and oil quality of rosemary. Results from the experiment revealed that among the seven treatments, the application of vermicompost (8 t ha^?1) + fertilizer nitrogen (N)?phosphorus (P)??potassium (K) (150:25:25 kg ha^?1) produced optimum herbage and oil yield of rosemary compared with control (no fertilizer) and was found to be on par with application of fertilizer NPK 300:50:50 kg ha^?1. Content and quality of oil were not influenced by vermicompost and chemical fertilizers. Furthermore, it was noticed that available N and P were greater in postharvest soils that received vermicompost alone or in combination with inorganic fertilizers than control (no fertilizer) and inorganic fertilizer?treated soil. This study indicates that combined application of vermicompost and chemical fertilizer helps to increase crop productivity and sustain the soil fertility.     

Influence of organic mulching and nitrogen application on essential oil yield and nitrogen use efficiency of rosemary (Rosmarinus officinalis L.)

In a field study conducted at Bangalore, India for two years (2003–2004 and 2004–2005), eight treatment combinations consisting of two variables, organic mulch (lemongrass spent material as mulch at 7.5 t ha^?1 and no mulch) and four levels of nitrogen fertilizer (0, 100, 200 and 300 kg ha^?1) were examined to observe the effect of organic mulching on nitrogen use-efficiency, and herb and essential oil yield in a multiharvested rosemary crop. The results revealed that application of lemongrass spent material as mulch increased the herb and essential oil yields in rosemary by 16.2 and 24.2%, respectively, over the non-mulched control at first harvest. Corresponding values for the regenerated crop harvest were 18.8 and 16.8%. A significant response to N was observed with 300 kg N ha^?1 in non-mulched plots compared with 200 kg N ha^?1 in mulched plots. Using lemongrass spent material as mulch, nitrogen uptake by the rosemary crop increased by 15.1% over the non-mulched control. At 200 kg N ha^?1, apparent recoveries by the crop were estimated to be 33.64% for the non-mulched control, and 37.79% with mulch. The quality of the rosemary essential oil, in terms of the concentrations of its major constituents, α-pinene, 1 : 8 cineole, camphor and verbenone, was not affected by the use of organic mulching and nitrogen fertilization, and these constituents were found to be of astandard acceptable in international trade.     

Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris)

The influence of nitrogen fertilizers on the yield of crop, as well as on the production and composition of the essential oil and some other chemical characteristics of thyme, was investigated. Different levels of fertilizers (N = 0, 45, 90, and 135 kg x ha(-)(1)) were applied. It was found that fertilizers increase thyme crop, but differences in the yield of essential oil were not remarkable. However, the use of certain amounts of nitrogen fertilizers resulted in higher yields of essential oil obtainable from the cultivation area unit (dm(3) ha(-)(1)). Totally, 61 constituents were identified in thyme essential oil by capillary GC and GC-MS. Thymol was the dominating compound in the all analyzed oils (44.4-58.1%), followed by p-cymene (9.1-18.5%), gamma-terpinene (6.9-18.9%), and carvacrol (2.4-4.2%). Differences in the percentage of these and other compounds in thyme herb cultivated under different fertilization doses were not significant; very slight changes in the percentage composition were detected after drying. Some variations in the amount of individual constituents expressed in arbitrary units per kilogram of herb (which is almost equivalent to mg x kg(-)(1)) were observed. The highest amounts of sugars and sucrose, in particular, were determined in the second year of thyme cultivation. Differences in the content of dry soluble substances were not meaningful, and there was no effect of nitrogen fertilizers on this chemical characteristic. Some effect of fertilization on the content of vitamin C and carotenes was observed in the first year of thyme cultivation. It was determined that nitrogen fertilizers influence the amount of nitrates, which was highest in the second-year-first-harvest.     

Influence of Nitrogen and Potassium Fertilization on Yield and Quality of Rosemary in Relation to Harvest Number

Rosemary is an important aromatic and spice plant. Its cultivation for production of its essential oil has gained importance in recent years. An experiment was conducted in a red sandy loam (Kandiustalf) soil to study the impact of cultivation of this multiharvest crop at different nitrogen (N) and potassium (K) application regimes on soil fertility and crop yield at the farm of the Central Institute of Medicinal and Aromatic Plants, Resource Center, Bangalore, during 2003–2005. The treatments consisted of all combinations of N levels (0, 150, and 300 kg per hectare per year) and K levels (0, 50, and 100 kg per hectare per year). Five harvests were taken at about 5‐month intervals. The results showed that initially the soil alone was able to meet the crop requirements for K, but with the progress of time, addition of K to the soil in the form of fertilizer became necessary for obtaining optimum yields. At the end of five harvests, exchangeable K in soil was significantly lower as application of K decreased from 100 to 50 and 0 kg per hectare per year. Soil fertility could be maintained and oil yields of rosemary from four harvests could be increased from 146.6 L to 344.7 L per hectare by application of 150 kg N and 100 kg K.     

Influence of Spent Mushroom Compost (SMC) as an Organic Fertilizer on Nutrient,Growth, Yield,and Essential Oil Composition of German Chamomile (Matricaria Recutita L.)

To investigate the effect of Spent Mushroom Compost (SMC) as an organic fertilizer on German chamomile (Matricaria recutita L.) nutrient, growth, yield, essential oil and osmolytes a greenhouse experiment was conducted through a randomized complete design in six replications. A mixture of sandy loam soil with 5, 10 and 15% volume rates of spent mushroom compost was used as the partial substrate for German chamomile pot culture. Finding of results revealed that usage of SMC in the growing media increased significantly plant growth, flower yield, essential macro nutrient uptake, sodium concentration, proline and soluble sugars content as well as essential oil percentages in compared to control. Comparison between the treatments indicated that addition of 10% SMC to the plant soil substrate cause to higher growth and addition of higher rates of SMC (10% and 15%) led to better yield. The obtained results showed that absorption of K and Na enhanced significantly by increasing of SMC percentage in growing media but there was no significant difference in N and P uptake in SMC treatments. The results of GC and GC-MS analysis presented that the main compositions of essential oil extracted from German chamomile flowers accounted for 83.99–99.84% of total essential oil in SMC treatments. Regarding important role of SMC on the essential oil yield, we can consider SMC could be a suitable substitute for chemical fertilizers as environmentally friendly material in cultivation medicinal plant German chamomile.     

Effect of nutrient solution salinity and ionic concentration on parsley (Petroselinum crispum Mill.) essential oil yield and content

The growth and essential oil (EO) production of parsley were evaluated in response to salinity and nutrient solution concentrations in a soilless culture. Parsley plants that were 60 days old were potted in a coconut fiber and peat moss medium and were treated with four different nutrient solutions, including T1, T2, T3 and T4. The T1 nutrient solution was the standard, the T2 and T3 solutions contained incremental macronutrient concentrations with an electrical conductivity (EC) of up to 2.2 and 3.2 dS m^?1, respectively, and the T4 solution was the same as T2 but with sodium chloride (NaCl) and an incremental macronutrient concentration with an EC of 3.2 dS m^?1. Next, these plants were grown for 90 days in a greenhouse with natural daylight in Nador, Morocco. Shoot and root growth significant decreased with increasing EC. However, the salinity that resulted from the addition of NaCl did not affect plant growth in the nutrient solutions. The optimum obtained growth and EO production were 1.2 and 2.2 dS m^?1, respectively. Consequently, the optimum EC value (based on the EO production) of parsley in the soilless culture was 1.2–2.2 dS m^?1.     

Variation of the chemical profile and antioxidant behavior of Rosmarinus officinalis L. and Salvia fruticosa Miller grown in Greece

In this study, the essential oil and the phenolic composition along with the antioxidant activity of R. officinalis L. and S. fruticosa Miller, collected in Zakynthos island (Ionian Sea, Greece), were investigated. The essential oil composition of the plants was characterized by the presence of 1,8-cineole. Mean values of the antioxidant activities of rosemary and sage essential oils indicated slight differences. The antioxidant activity of sage oil was correlated with the oxygenated sesquiterpenes and diterpenes concentrations. Concerning the methanolic extracts, a close relationship between the phenolic content and the development stage during vegetative cycle of these plants was observed. The identified flavonoids, except rutin, seemed to increase with the advancement of developmental stages, while phenolic acids followed an opposite pattern. The antioxidant activity was correlated with the amount of total phenolic content.     

栽培方式与钾互作对食用向日葵产量、品质和钾素利用率的影响

  【目的】  研究阴山北麓旱作区不同栽培方式下钾肥施用量对食用向日葵钾肥效应、产量性状、钾素吸收利用、油分品质和土壤–植物系统钾素平衡的影响，为向日葵上钾肥的科学施用提供理论依据。  【方法】  2014—2016年在内蒙古阴山北麓旱作区以食用向日葵 (3638C) 为研究对象，采用田间定位试验方法，裂区设计，主因素为3种栽培方式:平作雨养种植 (R)、全覆膜垄膜沟植集雨 (RC) 和全覆膜垄膜沟植滴灌 (I)。副因素为4个施钾水平:0、48、84和120 kg/hm2，表示为K₀、K₄₈、K₈₄和K₁₂₀。研究水钾互作对食用向日葵籽粒产量、产量性状、钾素吸收、钾肥利用效率、油分品质及土壤钾素平衡等的影响。  【结果】  栽培方式是影响向日葵籽实产量、花盘直径、千粒重、出仁率、钾素吸收量和钾肥利用效率的主要因素，3个栽培处理表现为全覆膜垄膜沟植滴灌 (I) > 全覆膜垄膜沟植集雨 (RC) > 平作雨养种植 (R)。全覆膜垄膜沟植滴灌 (I) 条件下，随着施钾量的增加，产量、花盘直径、千粒重、出仁率和钾素吸收量也增加，推荐施钾量 (K₂O) 为120 kg/hm2时，产量、花盘直径、千粒重、出仁率和钾素吸收量最高；全覆膜垄膜沟植集雨 (RC) 条件下，推荐施钾量 (K₂O) 为84 kg/hm2时，产量最高；平作雨养 (R) 条件下各施钾处理之间的产量、花盘直径、千粒重差异不显著。钾肥利用率和农学效率随着施钾量的增加而降低。栽培方式对籽实含油率影响较小，水分条件的改善有利于亚油酸含量的增加。随着钾肥用量的增加向日葵籽实的含油率也相应增加，主要是增加了亚油酸的含量。全覆膜垄膜沟植滴灌 (I) 条件下，推荐施钾 (K₂O) 量为120 kg/hm2时，土壤–植物系统的钾素基本平衡；全覆膜垄膜沟植集雨 (RC) 条件下，推荐施钾 (K₂O) 量为84 kg/hm2时，土壤–植物系统的钾素基本平衡；平作雨养 (R) 条件下，推荐施钾 (K₂O) 量为48 kg/hm2时，土壤–植物系统的钾素基本平衡。  【结论】  在内蒙古阴山北麓旱作区，全覆膜垄膜沟植滴灌 (I)、全覆膜垄膜沟植集雨 (RC) 和平作雨养种植 (R) 的推荐施钾 (K₂O) 量分别以120、84、48 kg/hm2为宜，此时，土壤–植物系统的钾素基本平衡。增施钾肥可增加向日葵籽实的含油率，主要是增加了籽实的亚油酸含量。     

Identification of the genotype from the content and composition of the essential oil of lemon verbena (Aloysia citriodora Palau)

Aloysia citriodora accessions from cultivated material, botanical collections, and wild populations were studied by means of their biomass and essential oil production and composition to assist the selection of the most promising genotype. The study was carried out through both field experiments during two year's time and laboratory processes. Data were evaluated by means of univariate and multivariate techniques. Aloysia citriodora intraspecific variation was accounted for by differences in both yield and chemical profiles of the essential oils, but no differences were found in the biomass production. Three chemotypes were identified according to qualitative and quantitative differences of the essential oils. For the higher contents of neral and geranial, Mendoza accession was the most promising to be encouraged for future crops.     

Low nitrogen availability and shallow plow layer decrease sugarcane (Saccharum officinarum L.) productivity in Kitadaito Island,Japan

Sugarcane is the main crop in Kitadaito Island, Okinawa, Japan. The average yield in Kitadaito Island for the past 30 years (1985–2015) was 44 Mg ha^?1, 31% lower than that (64 Mg ha^?1) in Okinawa Prefecture. Among the reasons for the low sugarcane productivity in Kitadaito Island, the effects of soil and cultivation factors have not been sufficiently studied. The objectives of this study were i) to evaluate the effects of soil physical and chemical properties and cultivation factors on sugarcane yield and sugar content in 50 fields of Kitadaito Island, and ii) to clarify factors affecting the sugarcane yield and sugar content. Statistical analysis of classification and regression trees (CART) showed that the sugarcane yield was low in fields with low available nitrogen (less than 16.5 mg kg^?1) and with shallow plow layer depth (less than 29 cm). The soil available nitrogen had a significant positive correlation with soil humus (r = 0.39, P < 0.05). Two-times of subsoiling in the field before planting and during growing periods resulted in the significant increase of the depth of plow layer, compared to the fields with single and no subsoiling. A high sugar content was obtained in fields with exchangeable Mg more than 3.8 cmol_c kg^?1 and pH(H₂O) less than 7.2. Our study therefore concluded that soil management practices for sugarcane production in Kitadaito Island should primarily be focused on available nitrogen, plow layer depth, and soil exchangeable Mg and pH.     

Supercritical fluid extraction and fractionation of different preprocessed rosemary plants

Two-step supercritical fluid extraction of rosemary leaves at selected conditions of pressure and temperature is proposed to divide the oleoresin into two fractions with different antioxidant activities and essential oil compositions. Rosemary leaves obtained from different sources have been extracted and evaluated in terms of antioxidant activity and essential oil yield and composition. Also, a new device is proposed to improve the performance of the technique in terms of sample collection after SFE.     

Grain oil and fatty acids composition of soybean affected by nano-iron chelate,chemical fertilizers and farmyard manure

Applying organic sources has been recommended to increase soil fertility and crop growth in sustainable production systems. Less information is available on the effects of organic inputs on soybean oil quality in the presence of nano-iron chelate. Field experiments were conducted during 2012 and 2013 to investigate the impact of organic, nano- and chemical sources on oil content and fatty acid composition of soybean (Glycine max [L.] Merr.). In split-plot arrangement of treatments, the main plots consisted of four fertilization systems: (F1): cattle manure, (F2): chemical fertilizers, (F3): cattle manure + chemical fertilizers, and (F4): control (without fertilizers). The subplots were (N1): soybean treated with nano-iron chelate fertilizer and (N2): control. Results showed that the highest oil yield was obtained in F3 treatment. The application of farmyard manure with nano-iron chelate significantly increased oleic acid compared to chemical fertilizers. The highest stearic acid and linolenic acid were observed in chemical fertilizer-contained treatments. Finally, the organic manure increased the oil quality of soybean.     

Soil properties and yam yield under different tillage systems in a tropical Alfisol

In Nigeria, information is lacking regarding the most suitable tillage method in extensive yam production. Hence, five tillage methods were compared at two sites in 2008–2010 with reference to their effects on soil physical and chemical properties, leaf nutrient concentrations, growth and tuber yield of yam (Dioscorea rotundata Poir) on Alfisols at Owo (site A) and Akure (site B), south-west Nigeria. The tillage methods were: zero tillage (ZT), manual ridging (MR), manual mounding (MM), ploughing + harrowing (P + H) and ploughing + harrowing + ridging (P + H + R). P + H + R had lower soil bulk density than other tillage methods and resulted in higher leaf N, P, K, Ca and Mg and yam tuber yield. In ZT, bulk density, soil moisture content, soil organic C, N, P, K, Ca and Mg were significantly higher and temperature lower than other tillage methods. Results of multiple regressions revealed that bulk density significantly influenced the yield of yam rather than soil chemical properties. Compared with MR, MM, P + H and ZT, and averaged across years, P + H + R increased yam tuber yield by 12.3, 12.8, 34.9 and 50.7%, respectively, in site A and 12.9, 13.5, 25.2 and 44.5%, respectively, in site B. P + H + R was found to be most advantageous and is therefore recommended for yam cultivation.     

The effect of N and NaCl on growth,yield, and nitrate content of salad rocket (Eruca sativa Mill.)

Hydroponic production of rocket as a salad vegetable has become increasingly important in recent years. Rocket is known to be a high nitrate (NO₃^–)-accumulating vegetable, which can be grown throughout the year. In the present study, rocket was grown in a floating hydroponic system at three levels of nitrogen (N) and sodium chloride (NaCl). The highest yield was obtained at 14 mM N, whereas the yield was lower at 20 mM and 40 Mm NaCl. Leaf elongation was more sensitive to salinity than leaf differentiation. Adding NaCl to the nutrient solution increased the relative chlorophyll content. Na⁺ and Cl^– concentrations increased as salinity increased. NO₃^? levels in fresh biomass increased with increased amounts of NO₃^? in the nutrient solution, and plants at 18 mM N were able to maintain a higher NO₃^? : Cl^? ratio than those at 10 mM N.     

Effects of biochar and poultry manure on soil properties,growth, yield and quality of cocoyam (Xanthosoma sagittifolium Schott) grown in sandy soil

ABSTRACT

Field experiments were conducted during the 2017 and 2018 cropping seasons, to evaluate the effects of biochar (B) and poultry manure (PM) on soil physical and chemical properties, leaf nutrient concentrations, growth, mineral composition and corm and cormel yield of cocoyam. The experiment each year consisted of 4 × 2 factorial combinations of B (0, 10, 20 and 30 t ha^?1) and PM (0 and 7.5 t ha^?1). Results of the study indicated that in both years, the application of B and PM alone, and in combination, improved soil physical and chemical properties, leaf nutrient concentrations, growth, mineral composition and corm and cormel yield of cocoyam. There was a significant interaction effect of B and PM (B x PM) which was adduced to the ability of the B to increase PM-use efficiency and promote better use of the nutrients in the PM. It was found that combination of 30 t ha^?1 B and 7.5 t ha^?1 PM (B₃₀+ PM_7.5) gave the highest corm and cormel yield of cocoyam compared with other treatments. The combination of 30 t ha^?1 B and 7.5 t ha^?1 PM (B₃₀+ PM_7.5) exhibited the highest impact and is therefore recommended for soil sustainability and cocoyam productivity on sandy soil.     

栽培模式对直播油菜生长、产量和养分吸收利用的影响

发展直播油菜对增加我国油菜种植面积和总产有重要意义,但栽培措施尤其是施肥技术的滞后极大地影响了油菜的产量及施肥效果。2009/2010年度在湖北省油菜主产区设置田间试验,研究栽培模式对直播油菜生长、产量、经济效益和养分吸收利用的影响,探讨适合当前生产的高产高效栽培技术。结果表明,各优化模式比农民习惯栽培模式均有增产增收效果,其中在30104 plant/hm2种植密度、秸秆还田和加强病虫草害防治的基础上进行优化施肥（氮、磷、钾肥用量分别为N 195 kg/hm2、 P2O5 90 kg/hm2、K2O 90 kg/hm2,硼砂用量15 kg/hm2,氮肥和钾肥分次施用）的模式Ⅲ表现最好,比农民习惯施肥增产35.9 %、增收1632 Yuan/hm2,地上部干物质量和养分累积也均较高,氮、钾肥偏生产力分别为N 13.7 kg/kg和K2O 29.8 kg/kg,实现高产高效目标。说明当前直播油菜的栽培模式应结合其生长发育进程和养分吸收规律,适当密植以增库促源,加强植保防治病虫草害,更重要的是积极推行平衡施肥和有机、无机配施,并合理安排施肥时期及比例。     

chemical composition, plant genetic differences, antimicrobial and antifungal activity investigation of the essential oil of Rosmarinus officinalis L

The chemical composition of the essential oil of the Sardinian Rosmarinus officinalis L. obtained by hydro distillation and steam\hydro distillation was studied using GC-FID and MS. Samples were collected at different latitude and longitude of Sardinia (Italy). The yields ranged between 1.75 and 0.48% (v/w, volume/dry-weight). A total of 30 components were identified. The major compounds in the essential oil were alpha-pinene, borneol, (-) camphene, camphor, verbenone, and bornyl-acetate. Multivariate analysis carried out on chemical molecular markers, with the appraisal of chemical, pedological, and random amplified polymorphic DNA data, allows four different clusters to be distinguished. The antimicrobial and antifungal tests showed a weak activity of Sardinian rosemary. On the other hand, an inductive effect on fungal growth, especially toward Fusarium graminearum was observed.     

Effect of phosphorus concentration of the nutrient solution on the volatile constituents of leaves and bracts of Origanum dictamnus

The chemical composition of the essential oils obtained from the leaves and bracts of hydroponically cultivated Origanum dictamnus were analyzed by GC-MS techniques. Three different concentrations of phosphorus (5, 30, and 60 mg/L) in the nutrient solution were used for the cultivation, using the nutrient film technique (NFT). A total of 46 different compounds were identified and significant differences (qualitative and quantitative) were observed between the samples. Carvacrol and p-cymene were identified as the main compounds in all samples analyzed, whereas thymoquinone was found in higher percentage in the leaves than in bracts. The essential oils were tested for their antibacterial activity against Gram-positive and Gram-negative bacteria. The oils obtained from the bracts were found to be more active. The results obtained from GC-MS analyses were submitted to chemometric analysis.