Effects of seedbed properties on crop emergence. 5. Effects of aggregate size,sowing depth and simulated rainfall after sowing on harmful surface-layer hardening

Abstract

Rapid, uniform crop establishment is a precondition for efficient crop production. In order to develop guidelines for seedbed preparation and sowing, extensive experiments were carried out in plastic boxes placed in the field directly on the ground for studies of the effects of seedbed properties on crop emergence. This paper deals with the effects on emergence of cereals caused by surface-layer hardening, induced by simulated rainfall (irrigation) after sowing followed by dry weather. The experimental crop was spring barley (Hordeum vulgare L.). Soils for the experiments (Eutric Cambisols, silt loam or clay loam in most cases) were collected from the surface layer of farm fields in various parts of Sweden. On soils with high silt content, irrigation after sowing often caused slumping and subsequent hardening of the whole seedbed. On clay soils, usually only a shallow surface crust formed. The earliest irrigation had the most negative effects on crop emergence. On a silt loam soil with unstable structure, irrigation with only 5 mm reduced emergence to under 20%. Later or heavier irrigation was often less negative, as it allowed the plants to emerge before the surface layer dried and hardened. Deep sowing greatly increased the negative effects on emergence, whereas soil aggregate size usually had negligible effects. It was concluded that when sowing in practice, seedbed preparation and sowing depth should be chosen to promote the fastest possible emergence. Sowing immediately before rain should be avoided, as should shallow sowing that requires rain for the seed to germinate.     

Effects of seedbed properties on crop emergence: 3. Effects of firming of seedbeds with various sowing depths and water contents

Abstract

Extensive experiments were carried out in shallow plastic boxes placed directly on the ground in the field to study the effects of seedbed properties on the emergence of various crops in a cool temperate climate. In a group of experiments with barley (Hordeum vulgare L.), we studied the effects on crop emergence of firming (slight compaction) of the seedbed, simulating the recompacting effects of rolling after sowing or press wheels on the seed coulters. Most of the experiments were carried out without irrigation after sowing. The firming usually had a slightly positive effect on emergence when the water content in the surface layer at sowing was below the wilting point. When the water content in the surface layer was higher, firming often reduced emergence drastically, particularly with deep sowing and in coarse-textured soils. The main reason for negative effects was harmful hardening of the surface layer when the more firm seedbed gradually dried out. When irrigation kept the seedbed continuously moist, the negative effect of firming was almost eliminated. Very high initial water content in the basal layer tended to delay surface layer drying and hardening, and to reduce the negative effect of firming. Firming only slightly influenced the evaporative water losses from the soil. In contrast to the results presented here, previous field trials with cereals have usually resulted in more positive effects of rolling after sowing. This indicates that other effects than firming, such as modification of sowing depth and reshaping of the soil surface, are other important effects of rolling.     

Effects of seedbed properties on crop emergence: 2. Effects of aggregate size,sowing depth and initial water content under dry weather conditions

Abstract

Rapid and uniform crop establishment is a prerequisite for efficient crop production and minimal environmental impact. Extensive experiments were carried out in shallow plastic boxes placed in the field directly on the ground for studies of the effects of seedbed properties on emergence of small-grain cereal crops in a cool, temperate climate. This paper presents studies of the seedbed as a protective layer against evaporation and the requirements for good emergence under dry weather conditions. Without rainfall after sowing, nearly complete emergence of barley (Hordeum vulgare L.) was obtained, when rapidly germinating seed was placed on a moderately compacted basal layer containing at least 5% (w/w) plant-available water and covered by a 4 cm deep surface layer consisting of aggregates < 5 mm. This applied even when initial water content in the surface layer was below the wilting point and potential evaporation was high. If the soil at 4 cm depth is drier or if a sufficiently fine seedbed cannot be obtained, then seedbed and sowing depth should be somewhat greater, and if the soil is wetter, then the depth may be smaller. There were only small differences in emergence between surface layers consisting of aggregates < 2 mm, 2–4 mm or 2–5 mm, or dominated by these aggregate fractions, but when the surface layer consisted of coarser aggregates, emergence was usually much lower. Determinations of water losses from the soil during the experimental period showed that the differences in emergence were caused by differences in efficiency of the seedbed to control evaporation. The results indicate that, in an initially wet soil, it is always possible to find a time and method for seedbed preparation and sowing that lead to good emergence of small-grain cereal crops, even with a lengthy period without rainfall after sowing.     

Effect of potato plants expressing snowdrop lectin (GNA) on the performance and colonization behaviour of the peach-potato aphid Myzus persicae

Abstract

Transgenic potato plants expressing snowdrop lectin (GNA potatoes) are developed to increase resistance against sap-feeding insects. When expressing GNA at relatively high levels such potatoes may have a negative effect on the fecundity and development of the first generation of the important pest, the peach-potato aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae). However, the effects on M. persicae over several generations, and how such plants affect the alate aphids’ colonization behaviour have not been reported. In this laboratory study, the performance of single M. persicae on potatoes with low GNA expression, measured as developmental time, fecundity, size and survival, was compared with the control, following two successive generations of single apterous aphids. Aphid population growth on the two plant lines was also studied. In addition, colonization behaviour was investigated in a choice experiment where the alate aphids could choose between the GNA and an isogenic control potato plant in a cage. The present study showed that the apterous aphid performance was not significantly different on the two potato lines, although the aphids tended to perform slightly poorer on the transgenic potato. However, the transgenic potato was less likely to be colonized by alate aphids. It is concluded that such transgenic potato plants expressing the lectin at a relatively low level, maximum 0.2% of the soluble protein, have no significant impact on the performance of apterous M. persicae once on the plant, but may have a potential in controlling the aphids by altering the colonization behaviour of alates.     

Impact of molybdenum on Chinese cabbage response to selenium in solution culture

Molybdenum (Mo) and selenium (Se) are both essential micronutrients for animals and humans. Increasing Mo and Se contents in food crops offers an effective approach to reduce Mo and Se deficiency problems. A hydroponic trial was conducted to investigate the interactions of Mo and Se on uptake, transfer factors (TF _shoot ) as well as distribution coefficients (DC) of Mo and Se on Chinese cabbage (Brassica campestris L. ssp. Pekinensis). In Experiment 1 three concentrations of Mo (0.01, 0.1 and 1?mg?L^?1) and four concentrations of Se (0, 0.01, 0.1 and 1?mg?L^?1) were arranged with a randomized block design. In Experiment 2, there were three treatments, 0.1?mg?L^?1 Mo, 0.1?mg?L^?1 Se and a combination of 0.1?mg?L^?1 Mo?+?0.1?mg?L^?1 Se. Experiment 1 showed that Se decreased Mo concentrations in shoots and roots. The impact of Mo on Chinese cabbage response to uptake of Se varied, depending on whether the root Se concentration was saturated or not; Experiment 2 showed that there is a strong antagonism between Mo and Se on nutrition uptake when Mo and Se deficiencies persist for long periods; Mo and Se were easily translocated from solution to plants and from roots to shoots. The results will also be of help in cultivating Mo-enriched and Se-enriched crops.     

Prediction of potentially mineralizable N from amidohydrolase activities in a manure-applied, corn residue-amended soil

Prediction of potentially mineralizable N as an important N pool from soil amidohydrolases was investigated. Composite soil samples were collected from plots of a field experiment in which 0, 50 and 100 Mg cow manure ha⁻¹ year⁻¹ had been applied for five consecutive years. The soils were treated with corn shoots or roots or remained untreated in a factorial combination with the manure treatments, with three replications. The mineralized inorganic N was measured periodically in 20-week incubations and potentially mineralizable N (N₀) was calculated based on a first-order kinetic model. Urease, l-glutaminase and l-asparaginase activities were measured before and after incubation. The values of N₀ ranged from 208.6 in the controls to 388.4 in soils that had received 50 Mg ha⁻¹ year⁻¹ of cow manure and were amended with corn shoots. Corn residue amendment in the manure treated soils, increased the values of N₀ or changed the N mineralization kinetic pattern from a first-order to a zero-order model. According to a relative sensitivity index, l-asparaginase was the most sensitive enzyme to the treatments. Multiple regression analysis revealed that 92% of N₀ variations can be described by the activities of urease and l-asparaginase and therefore the soil amidohydrolase activities have the potential to evaluate potentially mineralizable N.     

Nitrogen balance in a soil‐wheat system under plow‐ and no‐tillage in the argentine humid pampa

Abstract

Changing conventional tillage to conservation tillage systems affects nitrogen (N) cycling in agroecosystems. Our objective was to evaluate the role of soil organic pools, specially plant residues, as sources‐sinks of nitrogen in an humid and warm temperate environment cropped to wheat, under plow‐ and no‐tillage. The experimental site was in the Argentine Pampa on a Typic Hapludoll. A balance‐sheet method was used: Nupt+Nres=Nsow+Nmin, where Nupt=N uptake by the crop at harvest; Nsow=soil mineral N as NH₄ and NO₃ at 0–90 cm depth, one month before sowing, plus N added as fertilizer; Nres=residual soil mineral N as NH₄ and NO₃ at 0–90 cm depth, at harvest; Nmin=N mineralized from humus and plant residues during wheat growing period. Nupt did not differ between tillage systems. Nitrogen supply by the mineral N pool, estimated by the difference Nsow‐Nres, was ca. 150 kg N ha^‐1 in both tillage systems. Plant residues decomposed and released N under both treatments. This organic N pool decreased 77% along the crop cycle. Nmin, calculated using the balance equation was 83 kg N ha^‐1, and did not differ between tillage managements, representing 35% of Nupt. This results highlight the importance of the organic pools as sources of N for wheat in the Humid Pampa. They also brink our attention on the importance for evaluate residue decomposition and humus mineralization in warm‐temperate regions when fertilizer requirements are determined, in order to minimize environmental hazard and economic losses by overfertilization.     

Grain yield increases in wheat and barley to nitrogen applied after transient waterlogging in the high rainfall cropping zone of western Australia

Nitrogen (N) is a major factor limiting grain production in the high rainfall zone (HRZ, 450–700 mm annual average rainfall of southwestern Australia (SWA). Transient waterlogging and leaching of applied N fertilizer are hazards faced in most years by crop producers. The major crops are wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), canola (Brassica napus L.) and lupin (Lupinus angustifiolius L.) grown in rotation. Two series of experiments involving, levels and timing of N fertilizer application and levels of plant population were done. The first series, in 2003–2004, consisted of 3 experiments in 3 growing seasons (early May to late-October) to measure the grain yield (GY) increase (response) of wheat and barley to various methods of N fertilizer application (methods of split N application were compared to N applied at sowing). The aim of the experiments was to determine the optimal N fertilizer application strategy for maximum GY and quality in situations where transient waterlogging was a frequent occurrence. The second series of four experiments, from 2007–2009, measured the GY of wheat sown at three levels of plant population to 4 levels of N applied after transient waterlogging (taken to be rainfall events in which >25 mm of rain was recorded in 24 to 48 hours).

Applying the N fertilizer after high rainfall and transient waterlogging (tactical N application) increased GY and protein percentage of grain compared to applying all of the N fertilizer at sowing. Where transient waterlogging was not frequent, applying the N after waterlogging was not always better than applying part of the N according to growth stage of the crop or according to fixed times after sowing. When the crop was water-logged three or more times, N uptake by the crop at anthesis and apparent fertilizer N recovery in the crop was substantially increased by applying the N after waterlogging compared to applying the entire N at sowing. This study found that a tactical N management strategy for the HRZ of SWA is to apply some N at sowing with subsequent applications made after heavy rainfall that leads to transient waterlogging. Split N fertilizer applied either according to time after sowing or to growth stage of the crop was equally effective for increasing GY in situations where waterlogging was less frequent.

The observation from these experiments, that grain yield increases due to splitting the N dose were associated with increases in ear numbers, lead to a further set of experiments where plant population was increased in conjunction with N applied after waterlogging events. The combined strategy of increased plant population with strategic N application decreased the amount of N required for maximum GY where more than 3 heavy rainfall events occurred in a growing season.

One practical outcome of this research is to indicate that farmers can withhold applications of N fertilizer after sowing in seasons when transient waterlogging does not occur.     

Effects of seedbed properties on crop emergence: 1. Temporal effects of temperature and sowing depth in seedbeds with favourable properties

Abstract

Rapid and uniform crop establishment is a prerequisite for efficient crop production and minimal environmental impact. Experiments were carried out in shallow plastic boxes placed directly on the ground in the field for studies of the effects of seedbed properties on emergence of various crops. This paper presents an analysis of the time required for germination and emergence under near-optimal seedbed conditions. The crops studied were barley, oats, wheat, pea, rape seed, white mustard, sugar beet, red clover and timothy. The time required for germination generally increased with size of the seeds, presumably because larger seeds needed more water to initiate germination. This applied both when comparing different crops and different seed sizes of the same crop. However, considerable differences occurred between seed lots of the same crop and there were greater differences between seed lots of the same crop than between the three small grain crops studied. Growth rate of the seedlings generally increased with seed size, presumably because of increased energy content in the seed. Consequently, the most rapid emergence was obtained from small seeds at shallow sowing and from large seeds at deep sowing. The crops studied had a minimum temperature for germination and seedling growth close to 0 °C. Under optimal seedbed conditions, thermal time required for 50% germination of barley was typically about 65 °C days over this base temperature and for seedling growth about 6 °C days cm^?1. From 4 cm sowing depth, about 80 °C days were required for emergence but with considerable variations between seed lots. For rape seed or white mustard about 40 °C days were required for germination and about 8 °C days cm^?1 for the seedling growth.     

Effects of seedbed structure and water content at sowing on the development of soil surface crusting under rainfall

This study was carried out to observe the dynamics of crust formation on the soil surface under field conditions and analyse the effects of seedbed structure and water content on soil surface crusting. Seedbed sensitivity to crusting was also estimated in the laboratory by stability tests on aggregates. We observed 57 plots during the sowings of spring and autumn crops in fields in Northern France (Estrees-Mons, 50°N latitude, 3°E longitude). The soil is an Orthic Luvisol according to the FAO classification (0.17–0.25 g g⁻¹ clay and 0.02 g g⁻¹ organic matter on average). Visual assessments in situ were performed and photographs taken of crust stages on delimited areas, each 5 mm of cumulated rainfall since sowing. In 2004–2005, the seedbeds were characterised by their distribution of aggregate sizes and tests of aggregate stabilities of surface samples kept with their water content at sowing. A penetrometer was used to measure crust resistance and estimate its thickness. These data were analysed to detect the cumulative rainfall values needed for the initiation and development of the successive stages of crusts. A fully developed structural crust (stage F1) required 13, 22, 27 mm cumulated rainfall respectively for seedbeds with proportions of clods over 2 cm ranging from 0 to 0.15 (fine seedbed), 0.15 to 0.30 (medium seedbed), >0.30 g g⁻¹ (coarse seedbed). Aggregate stability measured on samples kept at sowing water content was low for soil with low water content (<0.17 g g⁻¹) but increased sharply for water contents over 0.17 g g⁻¹. Stage F1 was reached more rapidly (only 11 mm versus 19 mm cumulated rainfall) only for fine seedbeds with less than 0.15 g g⁻¹ of clods over 2 cm and with a low water content at sowing, The stage of 50% of soil surface covered with sedimentary crusts was reached for 85 mm for fine seedbed versus 120 mm for medium seedbed. The mean penetrometer resistance of dry crusts was 0.55 ± 0.43 MPa for stage F1 and 3.54 ± 0.83 MPa for a sedimentary stage; mean penetrometer resistance increased continuously with cumulated rainfall and was much lower for wet crusts. These quantitative data gathered under field conditions constitute the first step towards the prediction of soil surface crusting. The cumulative rainfalls were used in order to estimate the risk of occurrence of structural and sedimentary crusts forming during crop emergence with several types of seedbeds.     

Effects of Topographic Factors of the Site on the Essential Oil Compounds of Artemisia aucheri Aerial Parts Grown in a Mountainous Region

In this study, the chemical compositions of the essential oils from the aerial parts of Artemisia aucheri, grown in a mountainous region in Ghamsar Province, central Iran, have been analyzed by using gas chromatography (GC)–mass spectroscopy (MS) to determine how they are affected by topographic factors (site direction and elevation). Plants were sampled at random in full flowering stage in a completely randomized (CR) design with three replications. The essential oil was extracted by a modified Likens–Nickerson's simultaneous distillation–extraction (SDE) technique. Analysis of the plant oils on the sites [in two directions, north–south (N/S) and east–west (E/W)] and five elevations resulted in 30 identified compounds. Of the oil samples collected, the 99.94% of the components at the N/S site and 99.89% at the E/W site were identified. The main component in the two directions (N/S and E/W) was α-thujone with 19.5% and 18%, respectively. Other significant components in the two directions include α-pinene, davanone, camphor, and camphene. The percentages of all these main compounds on the N/S site were more than on the E/W site. Also, the main compounds increased with increasing altitude and at all five elevations. Among all the oil compounds, the percentages of α-thujone, α-pinene, and camphor were the greatest. Finally, at all the elevations, the percentage of the α-thujone was the greatest among the different oil compounds.     

Optimizing seed crops of common bent (Agrostis capillaris) by sowing rate

Abstract

As part of a project to stimulate Norwegian seed production of common bent (syn. browntop, US: colonial bentgrass, Agrostis capillaris L. syn. A.tenuis Sibth.) field trials comparing sowing rates of 2.5, 5.0, 7.5 or 10 kg ha^?1 were conducted at Landvik, south-east Norway, (58°N) from 1989 to 1994. Three trials were laid out of the forage cultivar ‘Leikvin’ and three trials of the lawn cultivar ‘Nor’, each trial being harvested for three consecutive years. While the average per cent ground cover in spring increased from 87% at 2.5 kg ha^?1 to 94–96% at 7.5 kg ha^?1, seed yields decreased with increasing sowing rate in both cultivars. On average for all harvests, quadrupling the sowing rate from 2.5 to 10 kg ha^?1 reduced seed yield by 9% in ‘Leikvin’ and 15% in ‘Nor’, the stronger effect probably being associated with a greater competition between tillers in the lawn cultivar. Seed yield reductions with increasing sowing rate showed no relationship with crop age, but were less accentuated for crops undersown in spring wheat in a dry year than for crops established without cover crop in years with ample rainfall in early summer. Increasing sowing rates reduced plant height and panicle number in ‘Nor’, but had no effect on seed weight or germination in any of the cultivars. It is concluded that seed crops of common bent should be established with a sowing rate of 2–5 kg ha^?1, with the lowest rate in lawn cultivars, under ideal seedbed conditions and when seed crops are sown without cover crop.     

A comparison between continuous and controlled grazing on a red duplex soil. II. Subsequent effects on seedbed conditions, crop establishment and growth

The effects of past grazing management practice on subsequent seedbed condition, draft requirements, fuel consumption, crop establishment and growth, and grain yield and quality were examined using three tillage systems on two sowing dates. The crop was wheat (Triticum aestivum), sown on a fragile sandy clay loam (red duplex soil) in a dryland agricultural area (307 mm average annual rainfall) of Western Australia. The three tillage-sowing systems investigated were: (i) scarifying followed by sowing with wide (180 mm) points; (ii) direct drilling with wide (180 mm) points; (iii) direct drilling with narrow (50 mm) inverted ‘T’-shaped Super-Seeder points. The two sowing dates provided differences in seedbed water content at sowing time. The three grazing management strategies practised in the previous pasture year were: (i) traditional set-stocking (where sheep were grazed continuously for 17 weeks, beginning soon after the start of the early winter rains); (ii) controlled grazing (where sheep were temporarily removed from the enclosure when the topsoil was close to its plastic limit); (iii) no grazing (where the pasture was mown to simulate grazing without trampling).Tillage prior to sowing with wide points reduced the mechanical impedance of the soil following set-stocking and provided a good seedbed for successful crop establishment and growth. In both the controlled-grazing management treatment and the treatment where the pasture had been mown the soil was suitable for direct drilling with both wide and narrow points (i.e. no pre-sowing tillage was required). The use of narrow points had the added advantage of requiring less fuel, but the need for a suitable implement to cover seeds was more critical than for wider sowing points. There were no advantages with respect to grain yield from adopting a controlled-grazing management practice owing to the lack of finishing rainfall. However, grain protein levels were higher in both the controlled and ungrazed treatments compared with the set-stocking treatment.     

Development of an appropriate procedure for estimation of RUSLE EI30 index and preparation of erosivity maps for Pulau Penang in Peninsular Malaysia

Pluviographic data at 15 min interval from 6 stations in Pulau Penang of Peninsular Malaysia were used to compute rainfall erosivity factor (R) for the revised universal soil loss equation (RUSLE). Three different modelling procedures were applied for the estimation of monthly rainfall erosivity (EI₃₀) values. While storm rainfall (P) and duration (D) data were used in the first approach, the second approach used monthly rainfall for days with rainfall ≥ 10 mm (rain₁₀) and monthly number of days with rainfall ≥ 10 (days₁₀). The third approach however used the Fournier index as the independent variable. Based on the root mean squared error (RMSE) and the percentage error (PE) criteria, models developed using the Fournier index approach was adjudged the best with an average PE value of 0.92 and an average RMSE value of 164.6. Further, this approach was extended to the development of a regional model. Using data from additional sixteen stations and the Fournier index based regional model, EI₃₀ values were computed for each month. ArcView GIS was used to generate monthly maps of EI₃₀ values and also annual rainfall erosivity (R). The rainfall erosivity factor (R) in the region was estimated to vary from 9000 to 14,000 MJ mm ha^− 1 h^− 1 year^− 1.     

Time of emergence of Rumex crispus L. as affected by dispersal time,soil cover,and mechanical disturbance

Abstract

Emergence pattern of the perennial weed species Rumex crispus L. was studied under semi-field conditions. Seeds from three populations were harvested from the mother plants and sown in pots buried in an experimental field, either in late autumn or after winter storage at outdoor temperatures. Seeds were sown on the soil surface or covered by a 2-cm soil layer. In addition, some of the seeds sown in autumn were also subjected to mechanical disturbance in autumn or in spring. Population and soil cover both had a strong effect on total emergence. The light requirement of the species for germination was indicated by a higher rate of emergence from seeds sown on the soil surface than from covered seeds. Sowing date influenced the timing of emergence but not the total emergence. Autumn sowing led to earlier and more concentrated emergence, while seedlings from spring-sown seeds showed a more intermittent emergence pattern. Stirring after sowing had a positive effect on emergence compared with emergence from undisturbed, covered seeds. In all populations and treatments, emergence continued throughout the growing season, contradicting earlier findings that R. crispus seeds germinate mainly in early spring and autumn, and enter secondary dormancy during summer. The delay of emergence in seeds that had remained on the mother plant over winter indicates a certain level of dormancy, which was gradually broken in early summer.     

Influences of Seedbed Bottom Properties on Seed Germination and Water Relations

Placement of seeds on a firm, moist seedbed bottom has proved important for crop establishment in spring in Sweden. However, most modern seed drills can place the seed at the desired depth independent of a firm seedbed bottom. This was not the case with older equipment. In this work, the importance of a firm seedbed bottom was evaluated, where germination of spring barley (Hordeum vulgare L.) under dry weather conditions was studied on a clay, a silty clay loam and a sandy loam with or without a firm seedbed bottom. The experiments were carried out in lysimeters protected from precipitation. Time-domain reflectometry was used for soil water content measurements, and thermocouples to monitor soil temperature. A firm seedbed bottom greatly improved seed germination on the clay soil with a small content of plant available water, but not on the other soils. On all soils, a loose seedbed bottom increased maximum surface soil temperature.     

Effect of tillage and residue management on enzyme activities in soils

Recent interest in soil tillage and residue management has focused on low-input sustainable agriculture. In this study we investigated the effect of three tillage systems (no-till, chisel plow, and moldboard plow) and four residue placements (bare, normal, mulch, and double mulch) on the activities of four amidohydrolases (amidase, L-asparaginase, L-glutaminase, and urease) in soils from four replicated field-plots. Correlation coefficients (r) for linear regressions between the activities of each of the enzymes and organic C or pH and between all possible paired amidohydrolases were also calculated. The results showed that the effects of tillage and residue management on pH in the 28 surface soil (0–15 cm) samples were not significant. The organic C content, however, was affected significantly by the different tillage and residue-management practices studied, being the greatest in soils with notill/double mulch treatment, and the least with no-till/bare and moldboard/normal treatments. Within the same tillage system, mulch treatment resulted in greater organic C content compared with normal or bare treatment. The activities of the amidohydrolases studied were generally greater in mulch-treated plots than in non-treated plots, and were significantly correlated with organic C contents of soils, with r values ranging from 0.70^*** to 0.90^***. Linear regression analyses of enzyme activities on pH values (in 0.01 M CaCl₂) of the 28 surface soils showed significant correlations for L-asparaginase, L-glutaminase, and urease, with r values of 0.74^***, 0.77^***, and 0.72^***, respectively, but not for amidase (r=0.24). The activities of the four amidohydrolases studied in the 40 soil samples tested were significantly intercorrelated, with r values ranging from 0.72^*** to 0.92^***. The activities of the four amidohydrolases decreased with increasing soil depth of the plow layer, and were accompanied by a decrease in organic C content.     

Effects of tillage, seeding method and time of sowing on the establishment of mungbean in drying, previously puddled soil

Two field experiments were conducted on small plots in the Philippines to determine the effects of tillage, seeding method and time of sowing on the establishment of mungbean (Vigna radiata (L.) Wilczek cv. IPB-M79-17-79) in seedbeds created in drying soil that had been puddled as for an immediately preceding wetland rice crop.

Conditions following rice were simulated by flooding, puddling and then draining the plots. Mungbean was sown at 2–14 days after draining (DAD) as the soil dried. In one experiment, seeds were sown manually into plots that were either non-tilled or for which the surface 10 cm had been ploughed and harrowed. In a second experiment, manual sowing into non-tilled plots was compared with prototype machine seeding. Soil matric potential and temperature were monitored throughout the experiments, and germination and seedling emergence recorded.

Surface cultivation slowed the rate of water loss from depths below 5 cm and resulted in lower thermal diffusivity than in non-tilled soil. Germination results indicated that following drainage of a seedbed in previously puddled soil, manual sowing at a depth of 5 cm could be delayed until 8 DAD (while soil matric potentials remained > − 0.1 MPa) without a significant reduction in seed germination. The seeding machine was quicker and easier to use, but its constraint of shallow sowing (maximum depth 2 cm) meant that sowing could be delayed only to 5 DAD before germination and emergence were inhibited. Predictions of germination from measured values of temperature and water potential were made using equations derived from controlled-environment studies. Differences from germination observed could probably be accounted for by seed/soil/water contact effects, which appeared to be especially important in dry soil (< − 0.7 MPa). Subsequent seedling emergence was, however, often severely restricted in non-tilled soil by soil mechanical constraints in the drying, strengthening seedbed. In the first experiment, these conditions were alleviated by the cultivation treatment; in the second, disturbance of surface soil before drainage resulted in greater emergence and faster seedling growth.     

Variable effects of emergence-promoting rhizobacteria on conifer seedling growth under nursery conditions

Summary Inoculation of white spruce (Picea glauca Voss.) seed with Bacillus polymyxa strain L5 under nursery conditions significantly increased the number of seedlings that emerged after sowing. No significant effects on seedling emergence were detected when white spruce seed was inoculated with Bacillus polymyxa strain L6, or when Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] seed was inoculated with either strain L5 or L6. However, white spruce seedlings originating from L5-inoculated seed had significantly lower root dry weights when measured 13 weeks after sowing, and reduced shoot dry weights 26 weeks after sowing compared with uninoculated controls. Inoculation of white spruce seed with strain L6 also resulted in seedlings with decreased root dry weights compared with uninoculated controls 13 weeks after sowing, but the significant inhibition of root growth was not apparent 26 weeks after sowing. Douglasfir seedlings originating from L5-inoculated seed had significantly lower root and shoot dry weights compared with uninoculated controls 13 but not 26 weeks after sowing. Inoculation of Douglas-fir seed with strain L6 resulted in seedlings with decreased root collar diameters and shoot dry weights 13 weeks after sowing, and lower root dry weights 26 weeks after sowing compared with uninoculated controls. These results demonstrate that the effects of bacterial inoculation on seedling emergence and on plant growth are independent, and that emergence-stimulating bacteria may inhibit subsequent seedling growth.     

Composition of amino acids,organic acids,and sugars in the peribacteroid space of soybean root nodules

In leguminous root nodules, bacteroids are differentiated from rhizobia and are surrounded by a peribacteroid membrane (PBM) forming an intracellular structure designated as symbiosome. Through the peribacteroid space (PBS) between the PBM and bacteroids, metabolic substances and signal compounds are exchanged between two symbionts. In this study, organic compounds with low molecular weight in the PBS were collected from isolated symbiosomes of soybean (Glycine max L.) root nodules, and their composition was analyzed and compared with that of the organic compounds in whole root nodules and bacteroids. Major differences were detected in the molar percentages of amino and organic acids, and sugars, to the total low molecular weight organic compounds among whole root nodules, PBS, and bacteroids. The PBS composition was characterized by abundant sugars and poor amino acids. Also the composition of the amino acids, organic acids, and sugars in the PBS was clearly different from that in whole root nodules and bacteroids. The PBS sugar composition was characterized by the predominance of inositols, especially myo-inositol at the 5th and 7th weeks of the host plant growth stages. Changes in the myo- and D-chiro- inositol balance at the host plant growth stages occurred and a syntony was observed between the PBS and bacteroids. The localization of myo-inositol in the PBS accounted for almost 70% of the total myo-inositol in root nodules. A small difference in the PBS composition between two soybean cultivars was recorded but it varied with the growth stages. It was tentatively concluded that the PBS sugar composition affected the bacteroidal sugar composition in soybean plants, and that inositol utilization in the bacteroids could be a factor controlling the bacteroidal function level which varied with the host plant growth stages.