玉米氮高效品种的生物学特征

提高氮肥利用率依赖于氮肥优化管理及作物氮素营养效率的遗传改良。本文分析了作物氮高效的定义,并以玉米为例,分析了氮高效的生物学机制,提出了玉米氮高效品种的生物学特征。本文认为,玉米氮高效品种的生物学特征为:(1)在开花前,维持稳定的氮吸收,并将所吸收的氮素高效利用于穗的发育,提高小花结实率,为产量形成过程中的碳、氮积累提供较大的库;根系生长发育能力强,能建成较大的根系,以满足籽粒生长期氮素吸收的要求;有较强的叶片扩展能力,保持较大的叶面积。(2)在开花后,充分利用前期建成的根系,高效吸收土壤中的矿化氮,用于籽粒生长所需,从而减少叶片中氮素的输出,减缓叶片衰老(保绿性强),维持叶片较高的光合效率,为籽粒灌浆提供碳化合物。因此,在氮高效育种中,应注重穗部性状(大穗,结实能力强)、根系性状(发达的根系,功能期长)与叶片性状(保绿性好)的结合。     

SUBSOIL ROOT GROWTH OF FIELD GROWN SPRING WHEAT GENOTYPES (TRITICUM AESTIVUM L.) DIFFERING IN NITROGEN USE EFFICIENCY PARAMETERS

In a two-year (1999–2000) field experiment four Swiss spring wheat (Triticum aestivum L.) genotypes (cvs. ‘Albis’, ‘Toronit’ and ‘Pizol’ and an experimental line ‘L94491’) were compared for genotypic differences in the root parameters that determine uptake potential and nitrogen use efficiency (NUE):root surface area (RSA) and its components, root length density (RLD) and the diameter of the roots. The genotypes were grown under no (N0) and under ample fertilizer nitrogen (N) [ammonium nitrate (NH₄NO₃); N1; 250 kg N ha^?1] supply. Root samples were taken from all the genotypes at anthesis from the subsoil (30–60 cm). Genotypic effects on RLD and RSA were evident only in 2000 and large amounts of N fertilizer usually diminished root growth. Adequate soil moisture in 1999 may have favored the establishment of the root system of all the genotypes before anthesis. Parameters of NUE for each genotype were also determined at anthesis and at physiological maturity. ‘Albis’ the least efficient cv. in recovering fertilizer N (ranged from 36.5 to 61.1%) with the lowest N uptake efficiency (0.47 to 0.79 kg kg^?1) had the lowest RLD and RSA in both seasons. Among genotypes ‘Toronit’, a high-yielding cv., efficient in recovering fertilizer N, exhibited the higher NUE (22.4 to 29.3 kg kg^?1) and tended to have the highest values of RLD and RSA. Nitrogen fertilization also led to an increase in the proportion of roots with diameters less than 300 μm and decreased the proportion of roots with diameters of 300 to 700 μm. These trends were more pronounced for cv. ‘Pizol’ in 1999 and for cv. ‘Toronit’ in 1999 and 2000. By anthesis in a humid temperate climate, there are no marked differences in the subsoil root growth of the examined genotypes. Some peculiarities on the root growth characteristics of the cultivars ‘Albis’ and ‘Toronit’ may partially explain their different NUE performance.     

不同滴灌施肥策略对棉花氮素吸收和氮肥利用率的影响

在温室条件下应用^15N标记尿素进行了不同滴灌施肥策略对棉花氮素吸收和氮肥利用率影响的盆栽试验.根据滴灌灌水施肥时段的分配,设置四种不同氮肥滴灌施肥策略.研究结果表明,不同滴灌施肥策略显著影响棉花的干物质重和氮素吸收量,棉花的氮素吸收量明显受到根系生长的影响,整株氮素吸收量与根干物质重之间呈显著的正相关关系.在一次灌溉过程中先滴1/2时间的肥液,然后再滴1/2时间清水的施肥策略可显著促进棉花根系的生长,增加棉花的氮素吸收量,减少氮肥在土壤中的残留,提高氮肥利用率.因此,在膜下滴灌条件下采用合适的施肥策略有助于提高肥料的利用效率.     

Durum Wheat Genotypic Variation of Yield and Nitrogen use Efficiency and Its Components Under Different Water and Nitrogen Regimes in the Mediterranean Region

Farmers in dry areas of the Mediterranean region are reluctant to apply nitrogen (N) fertilizer to rainfed wheat because of frequent drought. So, it is desirable to select varieties with high nitrogen use efficiency (NUE). The objectives of this study, conducted in 2009/10 and 2010/11 in Syria, were to study the response of genotypes of durum wheat to low and high N applications and to evaluate the contribution of N uptake efficiency and utilization efficiency to NUE under rainfed and supplemental irrigation conditions. Under the rainfed regime, grain yield decreased significantly in year 1 and year 2 when applying N fertilizer at a high rate. The early maturing genotypes gave the highest average yields under rainfed and irrigated regimes. High N fertilizer rate decreased significantly NUE from 36.1 to 24.3 in year 1 and 37.0 to 5.8 in year 2. Under irrigation, NUE fell from 84.6 to 67.1 in year 1 and from 117.7 to 33.3 in year 2. The contribution of N uptake efficiency and utilization efficiency to NUE varied from one year to another. In year 1, the contribution of N utilization efficiency was more important at all nitrogen levels; while the opposite was observed in year 2 when more N was applied. The fractions of the genotype sum of squares, respectively, for N uptake efficiency and utilization efficiency were in average 0.15 and 0.78 in year 1 and 0.75 and 0.25 in year 2. From this study we conclude that high N levels in the soil exacerbate the effect of water stress on productivity and NUE of wheat. Early maturing new genotypes tend to be better adapted and to use nitrogen more efficiently under limited water conditions. N utilization efficiency contributes more to NUE under high N availability than N uptake efficiency and vice-versa.     

Nitrogen Leaching Of Spring Wheat Genotypes (Triticum Aestivum L.) Varying In Nitrogen-Related Traits

Efficient use of nitrogen (N) by wheat crop and hence prevention of possible contamination of ground and surface waters by nitrates has aroused environmental concerns. The present study was conducted in drainage lysimeters for three years (1998–2000) to identify whether spring wheat genotypes (Triticum aestivum L.) that differ in N-related traits differ in N leaching and to relate parameters of N use efficiency (NUE) with parameters of N leaching. For this reason two spring wheat cultivars (‘Albis’ and ‘Toronit’) and an experimental line (‘L94491’) were grown under low (20 kg N ha^?1) and ample N supply (270 kg N ha^?1). The genotypes varied in parameters of NUE but not in N leaching. Grain yield of the high-protein line (‘L94491’) was, on average, 11% lower than that of ‘Toronit’ but among genotypes had significantly higher N in the grain (%), grain N yield, and N harvest index. Nitrogen lost through leaching was considerably low (0.42–0.52 g m^?2) mainly due to low volume of percolating water or the ability of the genotypes to efficiently exploit soil mineral N. There were no clear relationships between N-related genotype traits and N leaching, but across all treatments significantly negative correlations between volume of leachate and the amount of N in the total biomass and grain N yield existed.     

根性状的共变性和可塑性驱动不同水稻基因型对氮环境的适应性

[目的]研究不同氮敏感基因型的植物生长与根性状之间的关系及其对氮环境变化的响应,能够从根性状变化的角度来揭示植物对环境变化的适应性,从而有助于水稻育苗基因型的选育.[方法]在宁夏引黄灌区,采用双因素(水稻基因型×氮水平)随机区组设计田间试验,选取12个水稻基因型(高氮敏感基因型和低氮敏感基因型各6个)作为供试材料,设置...     

不同品种油菜氮效率差异及其生理基础研究

采用盆栽土培试验,以氮胁迫与正常供氮条件下的子粒产量比值作为氮效率系数,探讨了不同品种油菜氮效率差异及其生理基础。结果表明,供试5个油菜品种的氮效率有显著差异,其氮效率系数的变化范围是0.37~0.69。在氮胁迫条件下,氮高效品种的植株含氮量与氮素累积吸收量、叶片叶绿素含量与硝酸还原酶活性、茎叶可溶性糖含量与硝态氮再利用量高于氮低效品种。在正常供氮条件下,高潜力品种的植株含氮量低于低潜力品种,但由于其生物量较高,氮素累积吸收量并不低于低潜力品种,甚至苗期的氮素累积吸收量高于低潜力品种;高潜力品种的叶片叶绿素含量与硝酸还原酶活性、茎叶可溶性糖含量与硝态氮再利用量高于低潜力品种。说明上述4种生理指标均可作为评价油菜氮效率及增长潜力差异的间接指标。     

Nitrogen Use Efficiency of Rice Under Cadmium Contamination:Influence of Rice Cultivar Versus Soil Type

There is a need for rice cultivars with high yields and nitrogen (N) use efficiency (NUE), but with low cadmium (Cd) accumulation in Cd-contaminated paddy soils. To determine the relative effects of rice genotype, soil type, and Cd addition on rice grain yield and NUE, a pot experiment consisting of nine rice cultivars was conducted in two types of paddy soils, red soil (RS) and yellow soil (YS), without or with Cd spiked at 0.6 mg kg^-1. The N supply was from both soil organic N pools and N fertilizers; thus, NUE was defined as the grain yield per unit of total crop-available N in the soil. Cd addition decreased grain yield and NUE in most rice cultivars, which was mainly related to reduced N uptake efficiency (NpUE, defined as the percentage of N taken up by the crop per unit of soil available N). However, Cd addition enhanced N assimilation efficiency (NtUE, defined as the grain yield per unit of N taken up by the crop) by 21.9% on average in all rice cultivars. The NpUE was mainly affected by soil type, whereas NtUE was affected by rice cultivar. Hybrid cultivars had higher NUEs than the japonica and indica cultivars because of their greater biomass and higher tolerance to Cd contamination. Reduction of NUE after Cd addition was stronger in RS than in YS, which was related to the lower absorption capacity for Cd in RS. Canonical correspondence analysis-based variation partitioning showed that cultivar type had the largest effect (34.4%) on NUE, followed by Cd addition (15.2%) and soil type (10.0%).     

Nitrogen Use Efficiency in Cacao Genotypes

Cacao (Theobroma cacao L) is mostly grown on soils with low natural fertility. On such soils nitrogen (N) is one of the most yield limiting nutrients for cacao. Information is lacking on N use efficiency in cacao. A greenhouse experiment was conducted to evaluate growth response and N use efficiency by two cacao genotypes. The genotypes used were TSH-565 and ICS-9 and N rates adapted were 0, 120, 240, 360, and 480 mg N /pot. In both genotypes, increasing levels of applied N improved growth (stem girth, dry weight of shoot and roots and shoot/root ratio), and concentration and uptake of N. Genotypes differed significantly for stem girth and ICS -9 produced greater stem girth compared with TSH-565. Nitrogen uptake had a linear relationship with root dry weight of the two genotypes. In both genotypes, increasing levels of applied N overall increased N-uptake efficiency (NEFF = N concentration in shoot x shoot/root), but decreased N-use efficiency by shoot and roots (NUE = g dry matter of shoot or root/mg N) and N-use efficiency of carbohydrate (NUEC = mg of total carbohydrates in shoot/mg of N in shoot). Both genotypes responded differently to applied N, despite the existence of close genetic relatedness between them. The method used here appears to be suitable method for identification of cacao genotypes that are efficient in uptake and utilization of N.     

Yield and nitrogen use efficiency of wheat increased with root length and biomass due to nitrogen,phosphorus, and potassium interactions

Balanced applications of nitrogen (N), phosphorus (P), and potassium (K) are known to increase grain yield of wheat but the impact of the interactions among N, P, and K on root growth and nitrogen use efficiency (NUE) have not been proven. The aim of this study was to investigate the effect of balanced applications of N, P, and K on the rooting patterns and NUE of wheat. Two glasshouse experiments were conducted. A rhizobox study was used to assess the impact of interactions among N, P, and K fertilisers on total root length, biomass, specific root length, root length density, N use efficiency (NUE), and N uptake efficiency of the shoots (NUpE_shoot) and N nutrition index. In a separate pot study, plants were grown to maturity to confirm the effect of the observed changes in root growth on NUE, NUpE_grain, and grain/biomass yield. In the rhizobox experiment when plants were supplied with N+P+K, total root biomass increased approximately six‐fold relative to plants grown with N alone or with no fertiliser. Plants exposed to N+P+K had NUpE_shoot and NUE values that were five and ten times higher, respectively, than plants that received just fertiliser N. Plants supplied with N+P or N+P+K had N nutrition indices close to one (N‐adequate), while plants that only received N had an index of 0.62 (N‐deficient). The pot study confirmed that the changes in root length and biomass in plants exposed to N+P+K resulted in significant increases in NUE, NUpE_grain, shoot biomass, and grain yield at maturity. Interactions among fertiliser N, P, and K played a critical role in influencing root biomass and length, which was associated with increases in NUE, NUpE_shoot and NUpE_grain.     

Identification of Phosphorous Efficient Germplasm in Oilseed Rape

ABSTRACT

Plant species and genotypes within one species may significantly differ in phosphorus (P) uptake and utilization when they suffer from P starvation. The objective of this research was to screen P-efficient germplasm of oilseed rape (Brassica napus L.) and analyze the possible mechanism responsible for P efficiency by two-steps screening experiments and validation of P efficiency. Phosphorus efficiency coefficient at seedling stage, namely, ratio of shoot dry weight under low P to that under adequate P (PECS) of 194 oilseed rape cultivars varied from 0.050 to 0.62 and was significantly related with shoot dry weight under low P level (r = 0.859??, P < 0.01). Oilseed rape cultivar ‘Eyou Changjia’ presented the highest P efficiency coefficient in each growth stage and had the highest seed yield at low P, whereas oilseed rape cultivar ‘B104-2’ was the most sensitive to low P stress among the 12 candidate cultivars obtained from the two-steps screening experiments. Under low P condition in validation experiments of soil and solution cultures, ‘Eyou Changjia’ could produce much more dry matter and acquire more P than ‘B104-2.’ Moreover, P efficient coefficient obtained from the pot experiment was comparable to those from the field experiment. This might be attributed to high P uptake efficiency for ‘Eyou Changjia’ when it suffered from low-P stress. Comparison of results from the hydroponics with those from the pot and field experiments led to the conclusion that the P uptake efficiency in the hydroponics is highly related to that in soil culture conditions. These results show that there are large genotypic differences in response to phosphorus deficiency in oilseed rape germplasm (Brassica napus L.) and ‘Eyou Changjia’ is P-efficient and ‘B104-2’ is P-inefficient. By comparing these results further, the mechanism responsible for P efficiency was suggested to be mainly due to high P uptake efficiency by forming larger root system, and improving the ability of mobilizing and acquiring soil P in P-efficient oilseed rape under the condition of P starvation.     

Impact of integrated application of biochar and nitrogen fertilizers on maize growth and nitrogen recovery in alkaline calcareous soil

Biochar application has been considered as a rich source of carbon which helps to improve the physico-chemical properties and fertility of the soil. In Pakistan, excessive use of nitrogen fertilizer is considered a serious problem, so it is of vital importance to examine the effect of biochar on soil with varying doses of nitrogen fertilizer. We hypothesized that addition of biochar to an alkaline calcareous soil could improve not only soil quality and crop yield but also nitrogen use efficiency (NUE), reducing the loss of nitrogen (N) in the form of denitrification, ammonia volatilization, and nitrate leaching. A pot experiment was conducted under 2-factorial completely randomized design having three replications to evaluate the NUE in biochar amended calcareous soil. Biochar was applied at the rate of 0%, 1% and 2% (w/w) in pots filled with 17 kg of soil using various levels of N (0%, 50% and 100% of recommended dose) on maize (Zea mays L.). Several soil quality indicators, uptake, and yield of maize were monitored. Biochar application significantly decreased soil pH, increased water-holding capacity, total organic carbon, maize yield, stomatal conductance, and nitrogen uptake in plant. The results of the study indicated that addition of biochar could not only decrease the use of inorganic fertilizers by improving its quality and yield as in our case biochar at the rate of 1% and N at the rate of 50% provided optimum output minimizing the economic cost eventually.     

Interactive effects of water and nitrogen stresses on nitrogen utilization efficiency,leaf water status and yield of corn genotypes

Abstract

Information on the combined effects of N and water stresses on corn (Zea mays L.) is of importance for selecting nitrogen efficient and drought tolerant genotypes. Greenhouse and field experiments were conducted to determine the interactive effects of N and water stresses on N utilization efficiency (NUE), leaf water status and yield of diverse corn genotypes. Three genotypes which normally rank differently for NUE in the field, were grown to the 9‐leaf stage in the greenhouse with four N rates of 0, 30, 60, and 90 mg/kg and two water regimes (stressed for 8‐d, and unstressed). In the field experiment, four genotypes with different NUE history, were grown with N rates of 0, 60, 120, and 180 kg/ha, and water regimes of irrigated and dryland. Maximum grain yield was obtained at different N rates for different genotypes and for different water regimes. The genotypes differed significantly in NUE as expected, but also for water use efficiency (WUE) in both experiments. Nitrogen x water regime interactions for NUE were not significant in either experiment. Water regime did not significantly change the rank among genotypes for NUE as the genotype x water regime interactions for NUE were not significant in either of the two environments. This should greatly simplify selection of corn genotypes for NUE. NUE paralleled WUE closely for the genotypes studied. There was 19% difference between the best and poorest nitrogen efficient hybrids in terms of grain produced per kg total N uptake. Increasing N rate further decreased the total water potential of water stressed plants. There was a significant N x water regime interaction for grain yield. The grain yield increases due to irrigation compared to dryland ranged from 23% to 84% for the genotypes studied. Leaf water and osmotic potentials decreased significantly as N rate increased in the dryland, but were the same over N rates in irrigated plants. Apparent remobilization of N from leaves to grain was the major source of grain N in all genotypes, however, there were differences between genotypes for the fraction of leaf N remobilized. Genotypic selection for NUE does not appear to be influenced by water regime, and may result in simultaneous selection for WUE.     

Agronomic Assessment of Nitrogen Use Efficiency in Spring Wheat and Interrelations with Leaf Greenness Under Field Conditions

Four spring wheat genotypes (Triticum aestivum L.) were grown without (N0 = 0 kg N ha^?1) and under ample (N1 = 250 kg ha^?1) nitrogen (N) fertilizer in field experiments in two seasons. The aim was to assess genotypic variation in N use efficiency (NUE) components and N-related indices during grain filling thus to identify superior wheat genotypes. Leaf chlorophyll (SPAD) readings at crucial growth stages were employed to help differentiate genotypes. Interrelations between yield and N-related indices with SPAD, where also assessed to explain possible pathways of improving NUE early in the growing season. Results showed that genotypic effects on NUE were mostly evident in 2000, a year with drier preanthesis and wetter postanthesis than the normal periods. ‘Toronit’ almost always had the highest biomass yield (BY) and grain yield (GY). Except in 1999 under N0, ‘L94491? showed the highest % grain N concentration (GNC). Genotypes affected SPAD at almost all stages and N fertilization delayed leaf senescence for all genotypes and growth seasons. Correlations between SPAD at different growth stages and GY, N biomass yield at maturity (NBYM) and GNC were significant (P≤ 0.001), positive and strong/very strong (>r = 0.7). N translocation efficiency (NTE) was inversely related to PANU (~r = ? 0.77, P≤ 0.001), suggesting that N after anthesis is being preferentially transported to the ears to meet the N demand of the growing grains. It is concluded that there is still a large potential for increased NUE by improved N recirculation, use of fast and inexpensive crop N monitoring tools and high yielding, N uptake efficient genotypes.

Abbreviations: NUE, Nitrogen use efficiency; SPAD, Minolta SPAD-502 chlorophyll meter, NHI, nitrogen harvest index; HI, Harvest index; NTE, N translocation efficiency from vegetative plant parts to grain; DMTE, dry matter translocation efficiency; CPAY, contribution of pre-anthesis assimilates to yield; PANU, Post-anthesis N uptake, d.a.s., days after sowing, N0, zero (0) kg ha^?1 applied N fertilizer, N1, 250 kg ha^?1 applied N fertilizer.     

油菜NO3-的吸收、分配及氮利用效率对低氮胁迫的响应

【目的】探究油菜NO3-的吸收、分配和对低氮胁迫的响应及其氮利用效率,为理解油菜在不同低氮胁迫下相关生理变化及其氮素利用效率提供科学依据。【方法】以常规油菜品种814为研究材料,采用砂培试验,在正常供氮水平（10 mmol/L）和低氮胁迫水平（3 mmol/L、1 mmol/L）下,研究油菜的根系特性、蒸腾作用对低氮胁迫的响应及其氮素吸收效率,并研究油菜NO3-的运输分配与同化对低氮胁迫的响应及其氮素利用效率。【结果】与正常供氮处理（10 mmol/L）相比,低氮胁迫处理（3 mmol/L、1 mmol/L）的油菜NO3-含量、全氮含量均显著下降,但（NO3-）叶/根、（全氮（%））叶/根显著升高,植株根系干物质重、根系吸收面积均显著下降,但根冠比显著升高。油菜植株在低氮胁迫下气孔导度和蒸腾速率显著增加,一方面促进植株对NO3-的捕获,另一方面也促使更大比例的NO3-分配在植物的地上部分,但植株的水分散失加剧,水分利用效率显著下降。低氮胁迫处理油菜根和叶中NR、GS活性与正常供氮处理之间的差异不显著或有增加,其叶绿素含量、光合速率均显著下降,但光合氮素利用率显著升高。【结论】在低氮胁迫条件下,油菜植株的氮素和干物质累积均显著下降,但NO3-在植株的地上部分分配比例的增加以及光合氮素利用率的升高促使植株的氮素利用效率显著提高。     

Early nitrogen deficiency favors high nitrogen recovery efficiency by improving deeper soil root growth and reducing nitrogen loss in wheat

ABSTRACT

A two-year field and micro-plot ¹⁵N-labelled experiment was conducted under two levels of N application rate (240 and 180 kg N ha^–1) with three basal N application stages [seeding (L0), four-leaf stage (L4), and six-leaf stage (L6)] to investigate the effects of reducing basal N application amount and postponing basal N fertilization period on wheat growth and N use efficiency (NUE). No significant differences were observed in grain yield, root growth and root morphology between the N180L4 and N240L0 treatments, while the root-shoot ratio of N180L4 was significantly improved. Postponing basal N application period increased the residual basal ¹⁵N in soil and reduced basal ¹⁵N loss, and N180L4 treatment favored the highest ¹⁵N recovery efficiency (NRE), mainly due to reduced ¹⁵N loss. Grain yield and basal NRE were significantly positively correlated with root dry weight in deeper soil layers (40–60 cm), and the contribution of root growth to improved grain yield and NRE increased with the downward distribution of the roots. Therefore, postponing the basal N fertilization period under N deficiency promotes deeper root growth during the post-jointing period and increases basal N uptake, as well as reducing basal N loss and increasing grain yield and NUE.     

Corn Tolerance to Atrazine and Cadmium and Sunflower to Cadmium in Soil and Hydroponic Culture

Abstract

Hydroponic culture is often used for screening plant tolerance or remediation efficacy to environmental pollutants. Field experiments are usually avoided because of soil and groundwater contamination; thus pot experiments are the next step. In this study, the effect of various levels of atrazine in corn and cadmium (Cd) in corn and sunflower on physiological traits was comparatively investigated in hydroponic and soil culture. Also, Cd absorption and transfer factor to shoots were determined in soil. Atrazine affected corn growth at about 20% at the rates of 2 mg L^?1 and 20 mg kg^?1. In both species, Cd was largely stored in the root (75–88%), and the transfer factor was higher in corn than sunflower (0.59 vs. 0.37, at 20 mg kg^?1). Cadmium threshold of 20% reduction in shoot growth, leaf area, and chlorophyll in corn was observed at about 20, 40, and 80 mg kg^?1, respectively. The ratio of hydroponic–soil pollutant rate that similarly affected plant growth was about 1:10 and 1:5 for atrazine and Cd, respectively. The linear correlation coefficient between traits was also studied.     

Genotypic Differences in Dry Bean Yield and Yield Components as Influenced by Nitrogen Fertilization and Rhizobia

Dry bean (Phaseolus vulgaris L.) is an important legume worldwide and nitrogen (N) is most yield limiting nutrients. A field experiment was conducted for two consecutive years to evaluate response of 15 dry bean genotypes to nitrogen and rhizobial inoculation. The N and rhizobia treatments were (i) control (0 kg N ha^?1), (ii) seed inoculation with rhizobia strains, (iii) seed inoculation with rhizobia strains + 50 kg N ha^?1, and (iv) 120 kg N ha^?1. Straw yield, grain yield, and yield components were significantly influenced by N and rhizobial treatments. Grain yield, straw yield, number of pods m^?2, and grain harvest index were significantly influenced by year, nitrogen + rhizobium, and genotype treatments. Year × Nitrogen + rhizobium × genotype interactions were also significant for these traits. Hence, these traits varied among genotypes with the variation in year and nitrogen + rhizobium treatments. Inoculation with rhizobium alone did not produce maximum yield and fertilizer N is required in combination with inoculation. Based on grain yield efficiency index, genotypes were classified as efficient, moderately efficient, and inefficient in nitrogen use efficiency (NUE). NUE defined as grain produced per unit N applied decreased with increasing N rate. Overall, NUE was 23.17 kg grain yield kg^?1 N applied at 50 kg N ha^?1 and 13.33 kg grain per kg N applied at 120 kg N ha^?1.     

籼粳杂交稻高效吸收氮素的相关机理研究

通过盆栽和水培实验研究了籼粳杂交稻甬优12（YY-12）、嘉优中科6（JY-6）和常规粳稻秀水134（XS-134）常规供氮（M-N）和低氮（L-N）下分蘖盛期水稻氮素利用效率差异，并初步探讨相关机理机制。结果表明：YY-12和JY-6在两种供氮水平下氮素吸收效率明显大于XS-134。与XS-134相比，YY-12和JY-6根系干物质累积明显增大，加之根孔隙度（POR）较大，使其根系径向泌氧量（ROL）明显增加。从而很可能造成YY-12和JY-6根际土壤氨氧化菌（AOB）数量和硝化强度明显增加；使YY-12和JY-6根际土壤矿质氮含量明显大于XS134。相关分析表明根际土壤矿质氮含量与水稻氮素吸收效率极显著正相关。因此，YY-12和JY-6氮素吸收效率高很可能与其种植使根际土壤硝化强度增加和有机氮矿化加剧紧密相关。另外，与M-N处理相比，YY-12和YY-6氮素吸收效率在L-N水平下明显增加，而XS-134无明显差异。主要原因为：与M-N相比，YY-12和YY-6在L-N水平下根系干物质累积和POR增加比例均大于XS-134；ROL和根际土壤硝化强度，以及根际土壤矿质氮含量下降比例小于XS-134。