蛹虫草多糖对小鼠抗疲劳作用的研究

采用雄性ICR小鼠随机分组,分别灌胃蒸馏水、不同剂量的蛹虫草(Cordyceps militaris)多糖(低、中、高剂量组分别每天灌胃75、150、450mg/kg BW),40d后建立疲劳模型,比较力竭游泳时间、肝肌糖原水平、乳酸含量和机体相关的乳酸脱氢酶和超氧化物歧化酶活力。结果表明:高剂量蛹虫草多糖能够显著延长小鼠力竭游泳时间,中高剂量多糖显著提高小鼠肝肌糖原水平,有效的遏制运动后小鼠乳酸生成量,而低剂量多糖显著增加运动后小鼠乳酸脱氢酶活力,3个剂量的多糖均能提高运动后小鼠血清中超氧化物歧化酶的活力。     

姬松茸发酵液对小鼠抗运动疲劳及肝组织抗氧化能力的影响

研究姬松茸(Agaricus blazei)发酵液灌胃小鼠,对小鼠抗运动疲劳及肝组织抗氧化能力的影响.试验结果表明,与对照组(10 mg/kg·d ddH2O)相比,姬松茸发酵液中剂量组(20mg/kg·d)、高剂量组(30 mg/kg·d)小鼠的负重游泳、爬杆、耐缺氧时间均显著延长,血清尿素氮和血乳酸含量显著降低,肝糖原储存量显著升高,肝组织中SOD、GSH-Px活性显著提高,MDA值显著下降.说明灌胃姬松茸发酵液,能提高小鼠抗运动疲劳功能和加强肝组织抗氧化能力.     

野生红菇子实体提取液的抗运动性疲劳作用

分析红菇(Russula vinosa)的抗运动性疲劳的作用。利用昆明小鼠进行灌胃红菇子实体提取液的处理方式,并且连续灌胃21 d后,分析2组小鼠游泳力竭时长、肝糖原水平、乳酸脱氢酶等参数改变情况,并基于此分析容县野生红菇子实体提取液的抗运动性疲劳效果。结果表明,对照组小鼠游泳至游泳力竭的时间为114.31min～184.53 min,且肝糖原的含量在1.32 mg·g~(-1)～1.42 mg·g~(-1),乳酸脱氢酶含量在1.96 mg·g~(-1)～2.08 mg·g~(-1)。相较于对照组,高剂量、低剂量试验组在经过连续21 d红菇子实体提取液灌胃处理后,小鼠游泳力竭时间明显优于对照组;肝糖原水平也明显提高,乳酸脱氢酶活力显著下降。结果说明红菇提取液可以增强小鼠适应运动负荷、疲劳消除、抗疲劳等能力,提高抗疲劳效果。     

红菇提取液抗运动性疲劳作用的研究

目的：探讨红菇抗运动性疲劳的功效。方法：以容县红菇子实体为材料,昆明小鼠为试验对象,采用不同剂量的红菇子实体提取液对试验组小鼠连续灌胃21d后,对试验组与对照组的小鼠游泳力竭时间、乳酸脱氢酶、肝糖原含量等指标进行检测,研究容县野生红菇子实体提取液抗运动性疲劳作用。结果：经21d连续灌胃红菇子实体提取液的小鼠负重游泳时间与对照组相比显著延长;肝糖原含量比对照组显著提高,乳酸脱氢酶活力比对照组显著降低。结论：红菇提取液均能增强机体对运动负荷的适应能力、抵抗疲劳的产生和加速疲劳的消除,具有一定的抗疲劳作用。     

枇杷叶多糖对小鼠抗疲劳能力的影响

以热水提取枇杷叶中的水溶性多糖,用灌胃方法研究了枇杷叶多糖对小鼠抗疲劳能力的影响。结果显示,服用枇杷叶多糖小鼠的游泳时间比对照组小鼠延长了37.4%。从而证实枇杷叶多糖是枇杷叶的功能成分之一,能明显提高小鼠的抗疲劳能力。     

构菌菌丝酒提物的药效学研究

用东莨菪碱造成小鼠记忆获得障碍或用亚硝酸钠破坏小鼠记忆巩固，用明暗箱法来：观察菌丝酒提物对小鼠学习记忆障碍的改善作用；用断头喘息法观察菌丝酒提物的抗氧化作用；用游泳法观察菌丝酒提物的抗疲劳作用。结果显示，小鼠灌胃菌丝酒提物能明显延长避暗实验中记忆障碍小鼠从明场进入暗场的潜伏期，减少错误次数；能显著延长小鼠断头喘息时间和小鼠在冷水中的游泳时间。说明菌丝酒提物具有较强的改善小鼠学习记忆障碍、抗氧化作用和抗疲劳作用。     

灵芝发酵茶提取液对小鼠抗疲劳作用的研究

试验采用BABL/c小鼠，按体重随机分为对照组和灵芝发酵茶高（4.198g/kg）、中(3.358g/kg）、低（2.798g/kg）剂量组。连续给样30d，末次给样30min后，进行小鼠负重游泳试验与测定血清尿素氮、血乳酸和肝糖原三个生化指标的抗疲劳试验。结果表明，一定剂量的灵芝发酵茶提取液有显著的抗疲劳作用。     

杏鲍菇多糖的抗疲劳活性研究

对杏鲍菇(Pleurotus eryngii)多糖的抗疲劳活性进行研究。试验结果显示,给药组的小鼠游泳时间和爬杆时间更长,且肌糖原和肝糖原含量明显超过对照组,但是对照组和给药组的小鼠体重和脏器系数并无明显差别。由此可见,杏鲍菇多糖在抗疲劳方面具备显著功效。     

杏鲍菇多糖抗运动性疲劳机制

以昆明鼠为试验动物,将小鼠分为4组,对照组灌胃生理盐水,低剂量组、中剂量组、高剂量组分别灌胃50 mg·kg~(-1)d~(-1)、100 mg·kg~(-1)d~(-1)、200 mg·kg~(-1)d~(-1)的杏鲍菇(Pleurotus eryngii)多糖溶液,进行爬杆试验和负重游泳试验,并测定相关生化指标。结果显示低剂量组、中剂量组、高剂量组的爬杆和游泳时间均比对照组有延长,差异显著(P0.05),超氧化物歧化酶活性及肝糖原含量高于对照组,血乳酸含量低于对照组,均有显著性差异(P0.05),表明杏鲍菇多糖能够增强运动耐力,有抗运动性疲劳作用。     

皱环球盖菇抗疲劳作用活性部位筛选

为了探讨和筛选皱环球盖菇(Stropharia rugosoannulata)抗疲劳作用和活性部位,将160只昆明种小鼠分为2批,每批8组(80%乙醇提取物、石油醚萃取物、氯仿萃取物、乙酸乙酯萃取物、正丁醇萃取物、水层、阳性对照和空白对照组),每组10只,以红景天胶囊为阳性对照,每日灌胃1次,给药30 d。一批小鼠用来观察记录负重力竭游泳时间;另一批小鼠游泳30 min后,摘除眼球取血测定血清中尿素氮(blood urea nitrogen,BUN)、乳酸(lactic acid,LA)、乳酸脱氢酶(lactate dehydrogenase,LDH)、肌酸激酶(creatine kinase,CK)的值;取血后摘出左后肢股四头肌和肝脏,计算肝脏系数,分别测定左后肢股四头肌中肌糖原(muscle glycogen,MG)含量与肝脏中肝糖原(liver glycogen,LG)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的值。结果表明:皱环球盖菇氯仿萃取物提高了52.93%的小鼠负重力竭游泳时间,也可明显提高LDH和SOD活力,提高肌肉组织与肝脏中糖原含量,小鼠体内LG含量达(11. 10±1.41) mg/g,运动后BUN、LA、CK、MDA水平不同程度下降,小鼠肝脏中MDA含量与空白对照组相比下降17.58%。皱环球盖菇具有抗疲劳作用,发挥抗疲劳作用的活性成分主要分布于氯仿分部。     

裂褶菌对小鼠抗疲劳作用的研究

以裂褶菌子实体干粉（试验Ⅰ组和试验Ⅱ组）和裂褶菌发酵液（试验Ⅲ组）饲喂小鼠，检测裂褶菌子实体干粉和发酵液对小鼠肝糖原含量、肌糖原含量、琥珀酸脱氢酶（succinate dehydrogenase，SDH）活力、乳酸脱氢酶（lactic dehydrogenase，LDH）活力、血清尿素氮（blood urea nitrogen，BUN）含量及血乳酸（lactic acid，LD）含量的影响。结果表明，试验Ⅰ组、试验Ⅱ组和试验Ⅲ组小鼠的肝糖原含量、SDH活力及LDH活力极显著高于对照组（饲喂100％基础饲料）（p〈0．01）；试验Ⅰ组和试验Ⅱ组的肌糖原含量极显著高于对照组（P〈0．01）；运动后试验Ⅰ组、试验Ⅱ组和试验Ⅲ组小鼠的BUN增量和LD的水平极显著低于对照组（P〈0．01），说明裂褶菌具有明显解除运动疲劳的作用。     

Transplantation of mouse fetus-derived MSCs alleviates physical fatigue in mouse

AIM:To observe the effect of mouse fetus-derived mesenchymal stem cell(MSC) transplantation on alleviating fatigue in mice. METHODS:MSCs were derived from the mouse fetus at 13.5 d. The 6-month-old BALB/c mice were randomly divided into non-swimming group, swimming control group and swimming+MSCs group. The mice in swimming+MSCs group were injected with fetus-derived MSCs through the tail vein, while the mice in non-swimming group and swimming control group were injected with equal volume of normal saline. The anti-fatigue activity was evaluated using a weight-loaded swimming test, along with the determination of blood urea nitrogen(BUN), blood lactic acid(BLA),and glycogen in the hepatic and muscular tissues 24 h later. The antioxidant activity was evaluated by determining the activity of superoxide dismutase(SOD) and the content of malondialdehyde(MDA). The cardiac functions were measured by echocardiography. RESULTS:Primary cultured cells were spindle-shaped in scattered colony. The cells in fifth passage grew parallelly or swirlingly. The cells expressed CD44 and CD105, but not CD34 or CD45. Inducing experiments showed that the cells differentiated into osteoblasts or adipocytes. Transplantation of MSCs alleviated fatigue in mice. In swimming+MSCs group, weight-loaded swimming time was longer, the levels of BUN, BLA, hepatic and muscular MDA were lower, and the levels of glycogen and SOD in the hepatic and muscular tissues were higher than those in control group. Stroke volume, cardiac output and left ventricular diastolic volume in swimming+MSCs group increased, indicating that the cardiac functions were enhanced. CONCLUSION: Transplantation of mouse fetus-derived MSCs alleviates physical fatigue.     

块菌胞外多糖合成机制和运动抗氧化作用

针对块菌胞外多糖合成机制和运动抗氧化作用进行研究,采用了超声波辅助热水浸提法来提取块菌胞外多糖,并进行了多糖的精密度和稳定性测试;给小鼠灌胃块菌胞外多糖样品溶液后进行了小鼠负重游泳试验,试验结果表明,块菌胞外多糖能够明显延长小鼠的肌肉耐力,具有显著的运动抗氧化作用。通过小鼠肝脏称重试验结果表明,块菌胞外多糖能够有效提高小鼠肝糖原的储备,具有显著的抗氧化活性。     

金针菇多糖对运动疲劳免疫恢复的影响

针对运动疲劳的恢复问题,以小白鼠为动物研究对象,采用了分组对比试验法,研究了金针菇多糖对小白鼠血乳酸、尿素氮和运动时间的作用;以20名运动员为人体研究对象,采用分组试验法,对比了金针菇多糖对运动员最大摄氧量的影响。对比试验结果表明,金针菇多糖能够延缓血液中的血乳酸、尿素氮累积,调节机体的自我代谢和调节恢复能力,可以有效缓解运动疲劳,有效补充体力,促进运动疲劳的恢复。     

云芝多糖特征及其对运动耐力的影响

对云芝多糖特征及其对运动耐力的影响开展研究。通过云芝多糖的室温稳定性试验表明,云芝多糖具有良好的室温稳定性;抑菌性试验表明云芝多糖具有广谱抑菌效果;小鼠负重游泳试验表明,云芝多糖对运动耐力的提高有一定作用。证明了云芝多糖是一种安全的多糖来源,能够提升运动耐力。     

桑黄多糖的研究进展

桑黄(Phellinus)是国际公认抗癌效果较好的大型药用真菌之一,桑黄代谢产生的多糖具有明显的抑制肿瘤和提高机体免疫力的功效,为当前新型抗癌药物研究领域的热点。就桑黄多糖的化学结构、提取纯化方式及其药理作用进行了综述,并总结了桑黄多糖的产品开发情况,旨在为开发桑黄功能保健品和药品提供理论依据。     

Dexmedetomidine reduces renal injury induced by lung ischemia/reperfusion in mice through inhibiting endoplasmic reticulum stress response

AIM:To investigate the effect of dexmedetomidine (DEX) on renal injury induced by lung ischemia/reperfusion (I/R) in mice and its relationship with endoplasmic reticulum stress response.METHODS:Healthy SPF male C57BL/6J mice,weighing 20~24 g,aged 8~10 weeks,were randomly divided into 5 groups (n=10 each):sham operation group (sham group),I/R group,atipamezole (Atip) group,DEX group,and DEX+Atip group.In vivo lung I/R model was established by occlusion of the left pulmonary artery for 30 min followed by 180 min of reperfusion in the mice.The Atip (250 μg/kg),DEX (20 μg/kg) and DEX+Atip were intraperitoneally infused into the mice before left pulmonary hilus was blocked in Atip group,DEX group and DEX+Atip group,and other operations were the same as I/R group.After experiment,the mice were killed,and the renal tissues were harvested to observe the morphological changes.The enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,and cell apoptotic index of the renal cells were also analyzed.The expression of c-Jun N-terminal kinase (JNK),caspase-12,CCAAT/enhancer-binding protein homdogous protein (CHOP) and glucose-regulated protein 78(GRP78) at mRNA and protein levels in the renal tissues was determined by RT-PCR and Western blot.RESULTS:Compared with sham group,the enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,renal cell apoptotic index,and the mRNA and protein levels of JNK,caspase-12,CHOP and GRP78 in I/R group were significantly increased (P<0.01),and the renal tissues had obvious damage under light microscope.Compared with I/R group,Atip group and DEX+Atip group,the enzymatic activity of caspase-3,serum creatinine and blood urea nitrogen,renal cell apoptotic index,and the mRNA and protein levels of JNK,caspase-12 and CHOP in DEX group were significantly decreased,and the expression level of GRP78 significantly increased (P<0.01).Furthermore,the renal tissue damage was obvious reduced.CONCLUSION:DEX effectively relieves the renal injury induced by lung I/R in mice,which may be associated with exciting α₂-adrenergic receptor and inhibiting endoplasmic reticulum stress response.     

冬虫夏草固体发酵物水提液抗疲劳作用初步研究

通过研究冬虫夏草(Ophiocordyceps sinensis)固体发酵物水提液对负重小鼠游泳时间的影响,初步探讨其抗疲劳作用。结果表明:与阴性对照组相比,灌胃喂养冬虫夏草固体发酵物水提液的小鼠负重游泳时间显著延长,证明在本试验条件下冬虫夏草固体发酵物具有显著的抗疲劳作用。     

桑黄液体发酵培养基优化的初步研究

实验研究了桑黄液体发酵培养基中不同营养组成对桑黄菌丝生长的影响。以菌丝生物量和多糖产量为主要指标,对桑黄液体发酵培养基进行了优化。通过单因素试验和正交试验筛选出了桑黄液体发酵的最佳培养基。单因素试验结果表明最适氮源为麦麸,最佳碳氮比为20:1;正交试验结果表明,优化培养基配方为玉米粉50g·L-1、麦麸50g·L-1、KH2PO42g·L-1、MgSO41g·L-1、VB1300μg·L-1、VB2400μg·L-1。