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1.
You-Xiu Zheng Ching-Chung Chen Chia-Jin Yang Shyi-Dong Yeh Fuh-Jyh Jan 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(2):199-209
A putative virus-induced disease showing chlorotic ringspots on leaves of Phalaenopsis orchids has been observed in Taiwan for several years. A virus culture, 91-orchid-1, isolated from a Phalaenopsis orchid bearing chlorotic ringspot symptoms was established in Chenopodium quinoa and Nicotiana benthamiana, and characterized serologically and biologically. The virus reacted slightly with the antiserum of Watermelon silver mottle virus (WSMoV) but not with those of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV) and Groundnut ringspot virus (GRSV). Isometric particles measuring about 70–100 nm were observed. Inoculation with isolated virus was conducted to confirm
that 91-orchid-1 is the causal agent of chlorotic ringspot disease of Phalaenopsis orchids. To determine the taxonomic relationships of the virus, the conserved region of L RNA and the complete nucleocapsid
gene (N gene) were cloned and sequenced. The sequence of conserved region of L RNA shares 83.8, 82.5, 64.4 and 64.9% nucleotide
identities and 96.5, 97.7, 67.3 and 67.6% amino acid identities with those of Peanut bud necrosis virus (PBNV), WSMoV, TSWV
and INSV, respectively, indicating that 91-orchid-1 is a tospovirus related to WSMoV. The complete nucleotide sequence of
the N gene determined from a cDNA clone was found to be 828 nucleotides long encoding 275 amino acids. Sequence analyses of
the N gene showed that 91-orchid-1 is an isolate of Capsicum chlorosis virus (CaCV) which has been reported to infect tomato
and capsicum plants in Australia and Thailand. 91-orchid-1 is therefore designated as CaCV-Ph. To our knowledge, this is the
first formal report of a tospovirus infecting Phalaenopsis orchids. 相似文献
2.
You-Xiu Zheng Bing-Nan Shen Ching-Chung Chen Fuh-Jyh Jan 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(1):1-5
A new disorder exhibiting flower crinkle on Phalaenopsis orchids bearing white flowers has been observed in Taiwan, China and Japan for several years. This disorder decreased the
flower longevity and was considered as a physiological syndrome. The objective of this study was to identify and characterize
the real causal agent of this new Phalaenopsis disorder. Five plants of Phalaenopsis hybrids “V3” (Phal. Yukimai × Phal. Taisuco Kochdian) with flower crinkle symptoms were collected and tested by enzyme-linked
immunosorbent assay with antisera against 18 viruses. The extract of leaves and flowers from one diseased plant (96-Ph-16)
reacted positively only to antiserum against Odontoglossum ringspot virus (ORSV), while those from the other four plants (96-Ph-7, 96-Ph-17, 96-Ph-18 and 96-Ph-19) reacted positively to the antisera
against ORSV and Cymbidium mosaic virus (CymMV). Five ORSV isolates, one each from flowers of those five diseased Phalaenopsis orchids, were established in Chenopodium quinoa. A CymMV culture was isolated from the flowers of one of the ORSV/CymMV mix-infected Phalaenopsis orchids (96-Ph-19). To determine the causal agent of the flower crinkle disease, healthy Phalaenopsis seedlings were singly or doubly inoculated with the isolated ORSV and/or CymMV. Results of back inoculation indicated that
ORSV is the sole causal agent of the crinkle symptom on petals of Phalaenopsis orchid. The CP gene of the ORSV isolates from this study shared 97.3–100% nucleotide identity and 96.2–100% amino acid identity
with those of 41 ORSV isolates available in GenBank. This is the first report demonstrating ORSV as the sole virus causing
flower crinkle disease on Phalaenopsis orchids. 相似文献
3.
Hiroyuki UGA Yuki O. KOBAYASHI Kyoji HAGIWARA Yohachiro HONDA Toshihiro OMURA Takahide SASAYA 《Journal of General Plant Pathology》2002,68(4):378-381
The causative virus (isolate No. 4) of gentian (Gentiana spp.) mosaic, which had been identified previously as Clover yellow vein virus (C1YVV) on the basis of host range and serological reactions, was re-identified as Bean yellow mosaic virus (BYMV) on the basis of the nucleotide sequences of the gene for the coat protein (CP) and the 3′-noncoding region, as well
as the predicted amino acid sequence of CP.
Received 16 April 2002/ Accepted in revised form 19 June 2002 相似文献
4.
5.
Kentaro Okuno Tomoko Hama Minoru Takeshita Naruto Furuya Yoichi Takanami 《Journal of General Plant Pathology》2003,69(2):138-142
A potyvirus, for which the name Japanese hornwort mosaic virus (JHMV) is proposed, was isolated from Japanese hornwort plants
(Cryptotaenia japonica) with mosaic disease symptoms. The virus was used to inoculate mechanically 34 plants belonging to 33 species of 10 families.
Of these species seven from two families were infected. Faint chlorotic spots appeared on the inoculated leaves of Chenopodium quinoa and C. amaranticolor, but no systemic infection occurred in these plants. JHMV systemically infected only Umbelliferae plants; they did not infect
26 other species in eight families. JHMV was transmitted in a nonpersistent manner by aphids (Myzus persicae). The virus was a flexuous rod-shaped particle about 750 nm in length. Sequencing the nucleotides in the 3′ terminal region
of JHMV revealed that the coat protein contains 280 amino acids with a molecular mass of 32.2 kDa. The nucleotide sequence
of the coat protein of JHMV had the highest similarity with that of Zantedeschia mosaic virus (83.3%) compared to those of
other potyviruses (57.0%–64.9%). An antiserum against JHMV reacted strongly with JHMV and weakly with Potato virus Y. These results indicate that JHMV is a new potyvirus.
Received: September 9, 2002 / Accepted: November 7, 2002
RID="*"
ID="*" The nucleotide sequence determined in this work appears in the DDBJ/EMBL/GenBank nucleotide sequence databases with
the accession number AB081518 相似文献
6.
7.
Eduardo Segundo Dietrich E. Lesemann Germán Martín María P. Carmona Leticia Ruiz Isabel M. Cuadrado Leonardo Velasco Dirk Janssen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):81-87
Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV.
The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide
identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides
of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the
nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows
that AmLMV is a distinct potyvirus closely related to Potato virus Y. 相似文献
8.
9.
Igor Koloniuk Josef Špak Karel Petrzik 《European journal of plant pathology / European Foundation for Plant Pathology》2008,122(3):447-450
The nucleotide sequence of the 3′-terminal part of the RNA1 genome segment of the M12 isolate of comovirus Turnip ringspot virus (TuRSV) was established. This isolate originated in 1989 in Moscow (Russia) from Chinese cabbage with Radish mosaic virus-like symptoms. Comparison of the M12 RNA polymerase amino acid sequence with that of Radish mosaic virus (RaMV) revealed
significant differences; these proteins are of different length and are only about 75% identical. On the other hand, the amino
acid sequence of the M12 RNA polymerase was more than 94% identical with that of TuRSV recently described in Toledo (USA).
We conclude that TuRSV occurs in Europe as well as in America and probably represents a new species of the genus Comovirus. 相似文献
10.
Zhong-Bin Wu You-Xiu Zheng Chiou-Chu Su Chung-Jan Chang Fuh-Jyh Jan 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(1):71-79
A putative virus-induced disease of pear (Pyrus pyrifolia var. Hengshen) showing symptoms of reduced size of foliage and leaf distortion was observed in orchards in central Taiwan
in 2004. The sap of symptomatic leaf samples reacted positively to an antiserum against Apple stem grooving virus (ASGV). Two virus cultures, designated as TS1 and TS2, were isolated from symptomatic pears. Flexuous filamentous virions
of ∼ 12 × 600 nm were observed in symptomatic pear leaves and purified virus preparations. Results of back inoculation of
pear seedlings with TS1 revealed that ASGV was the causal agent of the disease. Sequence analyses of the cloned coat protein
(CP) genes of TS1 and TS2 shared 88–92.4% nucleotide and 90.7–97.1% amino acid identities with those of other ASGV isolates
available in GenBank. The polyclonal antibody generated against ASGV TS1 has been routinely used for the detection of the
ASGV-infection in the imported pear scions for quarantine purpose via enzyme-linked immunosorbent assays (ELISAs). One of
1,199 samples of pear scions imported from Japan during 2005–2007 was identified as ASGV-positive and the virus was designated
as AGJP-22. The CP gene amplified from this AGJP-22 shared 97.9–98.3% amino acid identities to those of the domestic isolates
and they were closely related phylogenetically. To date, these data present for the first time conclusive evidence revealing
that ASGV is indeed the causal agent of the pear disease displaying symptoms of reduced size of foliage and leaf distortion
in Taiwan. 相似文献
11.
Djabbar Hariri Michel Meyer 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(1):1-10
In April 2001, stunted barley plants bearing mosaic symptoms were observed in a field in France (Marne Department, 51). Rod-shaped
and flexuous particles were visualized by electron microscopy and positive serological reactions were detected by ELISA with
Barley yellow mosaic virus (BaYMV) and Soil-borne cereal mosaic virus (SBCMV) polyclonal antisera. The tubular virus which was soil transmissible to barley cv. Esterel was separated from BaYMV
by serial mechanical inoculations to barley cv. Esterel. This furo-like virus, in contrast to a French isolate of SBCMV, could
be transmitted to Hordeum vulgare, Avena sativa, Beta vulgaris and Datura stramonium. RT-PCR was used to amplify the 3′-terminal 1500 nucleotides of RNA1 and the almost complete sequence of RNA2. Nucleotide
and amino acid sequence analyses revealed that the French virus infecting barley is closely related to a Japanese isolate
of Soil-borne wheat mosaic virus (SBWMV-JT) which was originally isolated from barley. This French isolate was named SBWMV-Mar. The 3′ UTRs of both RNAs can
be folded into tRNA-like structures which are preceded by a predicted upstream pseudoknot domain with seven and four pseudoknots
for RNA1 and RNA2, respectively. The four pseudoknots strongly conserved in RNAs 1 and 2 of SBWMV-Mar show strong similarities
to those described earlier in SBWMV RNA2 and were also found in the 3′ UTR of Oat golden stripe virus RNAs 1 and 2 and Chinese wheat mosaic virus RNA2. Sequence analyses revealed that the RNAs 2 of SBWMV-Mar and -JT are likely
to be the product of a recombination event between the 3′ UTRs of the RNAs 2 of SBWMV and SBCMV. This is the first report
of the occurrence of an isolate closely related to SBWMV-JT outside of Japan. 相似文献
12.
Tri Asmira Damayanti Desmiarti Susilo Siti Nurlaelah Dewi Sartiami Tetsuro Okuno Kazuyuki Mise 《Journal of General Plant Pathology》2008,74(6):438-442
Severe mosaic with leaf malformation and green vein banding was observed on yam bean in West and Central Java, Indonesia.
Virions of the causal virus were flexuous filaments, about 700 nm in length, with a coat protein of 30 kDa. The virus was
transmitted by mechanical inoculation and by aphids in a nonpersistent manner. The nucleotide sequence of the coat protein
gene had the highest identity with that of Bean common mosaic virus (BCMV, genus Potyvirus) isolate VN/BB2-5. Based on demarcation criteria, including the genome sequence and host range, we tentatively designate
this isolate as BCMV-IYbn (Indonesian yam bean).
The nucleotide sequence reported is available in the DDBJ/EMBL/GenBank databases under accession number AB289438. 相似文献
13.
O. O. Odedara J. d’A. Hughes A. C. Odebode B. O. Odu 《Journal of General Plant Pathology》2008,74(4):322-325
Leaf samples of Lablab purpureus collected from two agroecological zones of Nigeria—the northern guinea savanna zone (NGSZ) and the derived savanna zone (DSZ)—were
infected with viruses when serologically indexed against available antisera. Approximately 31.1 and 81.1% of the leaf samples
collected from the NGSZ and DSZ, respectively, were infected. Seven viruses were found: Bean common mosaic virus (BCMV), Cowpea aphid-borne mosaic virus (CABMV), Cucumber mosaic virus (CMV), Cowpea mottle virus (CPMoV), Cowpea severe mosaic virus (CPSMV), Southern bean mosaic virus (SBMV) and Tobacco mosaic virus (TMV) were detected from samples collected from NGSZ, while CMV, CPMoV, Cowpea mosaic virus (CPMV) and CPSMV were detected from samples from DSZ. 相似文献
14.
A. G. Moreira E. W. Kitajima J. A. M. Rezende 《Journal of General Plant Pathology》2010,76(2):172-175
A Carica papaya plant with severe yellow leaf mosaic, leaf distortion, and systemic necrosis was found in the municipality of Piracicaba, state of São Paulo, Brazil. Transmission electron microscopy (TEM) analysis revealed the presence of potyvirus-like particles and bacilliform particles similar to those of the Alfamovirus genus. The potyvirus was identified as Papaya ringspot virus-type P (PRSV-P). Biological, serological, and molecular studies confirmed the bacilliform virus as an isolate of Alfalfa mosaic virus (AMV). Partial nucleotide and amino acid sequences of the coat protein gene of this AMV isolate shared 97–98% identity with the AMV isolates in the GenBank database. This report is the first of the natural infection of papaya plants by AMV. 相似文献
15.
K. S. Kubo J. Freitas-Astúa M. A. Machado E. W. Kitajima 《Journal of General Plant Pathology》2009,75(3):250-255
Brevipalpus-transmitted viruses (BTV) cause chlorotic, necrotic and/or ringspot lesions in leaves and stems of orchids, citrus, coffee
and several other plant species. There are two different types of BTVs, the nuclear and the cytoplasmic, based on maturation
locale in the cell and particle morphology. The orchid fleck virus (OFV) is a BTV that infects orchids. Its short rodlike
particles are 32–40 nm in diameter, 100–150 nm in length. OFV is found in the nucleus and is associated with intranuclear
electronlucent viroplasms. In 1999, transmission electron microscopy analysis revealed a distinct type of virus causing orchid
fleck symptoms. The bacilliform particles, 70–80 nm in diameter and 110–120 nm in length, induced electron-dense viroplasm
inclusions in infected cells and resembled the cytoplasmic type associated with BTV, such as the citrus leprosis virus C.
Our objective in the present study was to verify whether the cytoplasmic type virus found in orchids could be amplified using
primers for other cytoplasmic BTVs, such as CiLV-C and Solanum
violaefolium ringspot virus (SvRSV). Additionally, we aimed to differentiate the two BTVs found in orchids: the nuclear and the cytoplasmic
types of OFV using microscopy and molecular and serological tools. This virus was not amplified by the CiLV-C and SvRSV primers,
and neither the molecular nor the serological tools available to the OFV diagnosis reacted with it, demonstrating that they
are definitely different viruses. 相似文献
16.
A. A. Khan R. Sharma B. Afreen Q. A. Naqvi S. Kumar S. K. Snehi S. K. Raj 《Phytoparasitica》2011,39(2):199-203
Natural occurrence of mosaic disease was observed on basil (Ocimum sanctum L.) in Aligarh, U. P., India, during 2008. The disease could be transmitted by sap inoculations from naturally infected O. sanctum to O. sanctum and some test plant species. Cucumber mosaic virus (CMV) was detected by RT-PCR using coat protein gene specific primers of CMV (Acc. AM180922 & AM180923), which resulted in
the expected size ~650 bp amplicon in infected samples. The amplicon was cloned, sequenced and data were deposited in GenBank
Acc. EU600216. The sequence data analysis revealed 97–99% identities at both nucleotide and amino acid levels with the CMV
strains of subgroup II reported worldwide. Based on the high sequence identities and close phylogenetic relationships with
CMV subgroup II strains, the virus under study has been identified as a new isolate of CMV subgroup II and designated as CMV-Basil. 相似文献
17.
Shu-Chuan Lee Ya-Chun Chang 《European journal of plant pathology / European Foundation for Plant Pathology》2008,122(2):297-306
Antisera against important orchid viruses, Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV), were separately produced using bacterially expressed recombinant capsid proteins (CP), instead of purified virus
particles, as immunogens. These antisera were then designated as home-made CymMV CP antiserum (HM-Cy) and home-made ORSV CP
antiserum (HM-OR). The high specificity of HM-Cy and HM-OR were confirmed by immunoblot. Their detection limits were determined
using indirect-enzyme-linked immunosorbent assay (I-ELISA). Both HM-Cy and HM-OR showed low background reactivity to healthy
plants and thus displayed a high S/H ratio (sample OD405/healthy control OD405) in tested orchids. The data indicated that
our antisera were efficient and accurate in determination of negative and positive results in ELISA test as commercial antibodies.
Therefore, these home-made antisera of CymMV and ORSV are suitable for the certification programme of orchids due to their
low cost and high specificity. HM-Cy and HM-OR were further used for a field survey to study the incidence of CymMV and ORSV.
The results showed that CymMV is more prevalent than ORSV in Taiwan. 相似文献
18.
<Emphasis Type="Italic">Turnip yellow mosaic virus</Emphasis> isolated from Chinese cabbage in Japan
Namiko Kirino Koji Inoue Koji Tanina Yuya Yamazaki Satoshi T. Ohki 《Journal of General Plant Pathology》2008,74(4):331-334
A virus that caused a distinct yellow mosaic was isolated in Okayama, Japan from Chinese cabbage (Brassica rapa L., Pekinensis group). The virus, with spherical particles ca. 28 nm in diameter, was mechanically transmissible only to
cruciferous species. From the host range, characteristic morphology of virus particles, serology and sequence analysis of
coat protein gene, the causal virus was identified as Turnip yellow mosaic virus (TYMV). Seed transmission of TYMV at 0–2.2% in Chinese cabbage was confirmed. This report is the first of TYMV from Chinese
cabbage and in Japan.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB358971 and AB358972. 相似文献
19.
20.
In 2009, chlorotic mottle and necrosis were observed on chrysanthemums (cv. Jimba) in Aomori Prefecture, Japan. A virus was
isolated from the chrysanthemum plants by serial local-lesion transfer. The symptoms exhibited by the test plants, the particle
morphology, the features of the protein and the potential for transmission by thrips were similar to those for Impatiens necrotic spot virus (INSV). The partial nucleotide sequences of the nucleocapsid protein gene and the 3′-untranslated sequence of the S RNA shared
99% identity with that of an INSV isolate. This report is the first of INSV infection of chrysanthemums in Japan. 相似文献