南京地区犬巴贝斯虫病的诊断与治疗

4年来,笔者在宠物门诊工作过程中,通过对161例犬巴贝斯虫病的诊治与跟踪调查(治愈148只,死亡13只,治愈率达91.93%),总结出发病规律和治疗方案,发现本地区犬巴贝斯虫病主要是由吉氏巴贝斯虫和犬贝斯虫感染所致。其中由吉氏贝斯虫感染的病例为106例,由犬巴贝斯虫感染的病例为55例,未发现韦巴贝斯虫感染的病例。针对犬巴贝斯虫病的发病特点,提出了预防新措施。     

一例藏獒犬巴贝斯虫继发肝硬化死亡病例的诊治体会

犬巴贝斯虫病是由吉布索尼氏巴贝斯虫和犬巴贝斯虫引起的、以蜱为媒介的、寄生于红细胞内的血液寄生虫病。临床上以严重贫血、高热、消瘦以及血红蛋白尿为特征。近几年来武邑职教中心动物门诊收治多例犬感染巴贝斯虫的病例,现将其中一印象深刻的病例治疗体会报道如下。     

犬巴贝斯虫病的流行与诊疗方案

正犬巴贝斯虫病是犬的一种血液寄生虫病,其病原体为巴贝斯虫或其他焦虫。临床上,本病主要表现为呼吸困难、高度贫血、黄疸、血红蛋白尿等特征。犬巴贝斯虫病在我国目前发病越来越多。1病原特征据报道,引起本病的病原主要有犬巴贝斯虫、吉氏巴贝斯虫及韦氏巴贝斯虫。犬巴贝斯虫和吉氏巴贝斯虫的感染在我国常见,但韦氏巴贝斯虫在我国尚未见报道。1.1犬巴贝斯虫犬巴贝斯虫寄生于犬的红细胞内。虫体为梨形,一端尖,另一端圆,内有一个空泡,典型虫体大于1/5红     

犬巴贝斯虫病的诊治体会

犬巴贝斯虫病是以蜱虫为传播媒介,由犬巴贝斯虫或吉氏巴贝斯虫所引起的一种血液原虫病,临床上以高热、贫血、黄疸和血红蛋白尿为主要特征。2005年以来,由于气候变化等因素,福建地区犬蜱虫病传播严重,以此为传播媒介的巴贝斯虫病也随之多发。笔者共参与诊治36例,治愈32例,死亡4例,治愈率为89%。     

犬巴贝斯虫病的诊治

犬巴贝斯虫病是一种经硬蜱传播的血液原虫病，临床上以严重贫血和高热为特征。本病的病原体为犬巴贝斯焦虫。犬巴贝斯虫病在我国报道很少，一般只在有蜱滋生的地区呈地方性流行。2002年我院曾收治过犬巴贝斯虫感染病例3例，为比特犬，经治疗后均痊愈。报道如下。     

犬巴贝斯虫病的诊治体会

犬巴贝斯虫病是以蜱虫为传播媒介,由犬巴贝斯虫或吉氏巴贝斯虫所引起的一种血液原虫病,临床上以高热、贫血、黄疸和血红蛋白尿为主要特征。2005年以来,由于气候变化等因素,福建地区犬蜱虫病传播严重,以此为传播媒介的巴贝斯虫病也随之多发。笔者共参与诊治36例,治愈32例,死亡4例,治愈率为89%。     

68例犬巴贝斯虫病病例研究

<正>犬巴贝斯虫病是经蜱传播的血液原虫病,自从1910年Pattom首次在印度报道了犬的巴贝斯虫感染猎犬和豹以后,世界各地相继发现该虫。巴贝斯虫病在我国呈地方性流行,尤其在有蜱滋生的地区,春、夏、秋、冬季均可发病[1]。目前随着城市养犬数量增多,本病的发病率也越来越高,危害十分严重。犬巴贝斯虫主要寄生于犬的血液,引起犬贫血、黄疸、血红蛋白尿和脾脏肿大[2]。笔者通过血涂片、血液常规检验、生化检验、X射线检查并结合病史和     

犬巴贝斯虫的诊疗与防控技术

<正>犬巴贝斯虫病是由巴贝斯虫引起的经蜱传播的寄生血液红细胞内的原虫病。临床特征为严重贫血和血红蛋白缺乏。对犬、特别是军、警犬危害严重。犬巴贝斯虫属于原生生物界,顶复门,孢子虫纲,梨形虫目,巴贝斯虫科,巴贝斯虫属。引起犬巴贝斯虫病的病原主要有3种,即犬巴贝斯虫、吉氏巴贝斯虫和韦氏巴贝斯虫。1犬巴贝斯虫寄生于犬红细胞内,虫体     

一例藏獒犬巴贝斯虫继发肝硬化死亡病例的诊治体会

<正>犬巴贝斯虫病是由吉布索尼氏巴贝斯虫和犬巴贝斯虫引起的、以蜱为媒介的、寄生于红细胞内的血液寄生虫病。临床上以严重贫血、高热、消瘦以及血红蛋白尿为特征。近几年来武邑职教中心动物门诊收治多例犬感染巴贝斯虫的病例,现将其中一印象深刻的病例治疗体会报道如下。1病例介绍2013年5月份,河北省衡水市武邑县一养犬者带着一只体重72 kg的藏獒来动物医院就诊。主诉     

河南省豫东地区犬巴贝斯虫病的病例研究

正犬巴贝斯虫病是一种以蜱为媒介的血液寄生虫病,病原主要有犬巴贝斯虫、吉氏巴贝斯虫和韦氏巴贝斯虫三种~([1])。犬巴贝斯虫感染的指示性临床特征为高热、贫血,特别在一些感染严重的病例中,血色蛋白尿是一个很好的指标~([2])。该病多发于蜱孳生地区,具有明显的季节性,在蜱虫活动频繁的春秋季节,宠物犬常因到草地牵遛或爬山而感染。目前检测犬巴贝斯虫最常见方法有直接血涂片观察虫体法、PCR检测方法和免疫学诊断法。笔者于2014年6—9月     

Babesia canis canis and Babesia canis vogeli clinicopathological findings and DNA detection by means of PCR-RFLP in blood from Italian dogs suspected of tick-borne disease

The aims of this study were to determine the presence of Babesia spp. in blood samples from Italian dogs with clinical signs compatible with tick-borne diseases by means of PCR-restriction fragment length polymorphism (RFLP) and describe the clinicopathological findings of dogs with Babesia infection. We evaluated the majority of canine babesiosis cases by means of clinical history, physical examination, hematological, biochemical, serum electrophoresis, urinalysis and hemostatic tests. Forty-five out of 164 canine blood samples studied were positive to Babesia PCR-RFLP with the following results: Babesia canis canis (n=34) and Babesia canis vogeli (n=11). The majority of B. c. canis infections were detected in Northern Italy (29.1%; 30/103). B. c. vogeli cases were detected mainly in Central and Southern Italy (16.3%; 10/61). Only one B. c. vogeli was detected in Northern Italy (0.9%; 1/103). Three positive samples to B. c. canis and four positive samples to B. c. vogeli were selected for sequencing of a fragment of the 18S rRNA gene (410bp) for further molecular characterization. The sequence obtained from all seven dogs was 99/100% homologous to sequences from B. c. canis and B. c. vogeli, respectively, present in GenBank. Sixty-two percent of dogs infected with B. c. canis had recently travelled on a hunting trip to East European countries. The main acute clinical signs were dehydration, apathy, anorexia and fever. The majority of dogs infected with B. c. canis presented at initial clinical examination mild to severe thrombocytopenia, hyperfibrinogenemia, mild to moderate normocytic-normochromic non-regenerative anemia, hemolysis and neutropenia. The urinalysis showed hemoglobinuria in 13/19 dogs suggesting intravascular hemolysis. Dogs with B. c. canis infection had high levels of C-reactive protein. Hypoalbuminemia was present in 17/26 dogs. The 11 cases of B. c. vogeli infection did not present a homogenous clinicopathological pattern. B. c. vogeli infections were observed in young dogs causing hemolytic anemia and in adult/old does that frequently presented predisposing factors such as splenectomy or immunocompromised conditions. In conclusion, this study demonstrates the presence of B. c. canis and B. c. vogeli in Italian sick dogs and differences in clinicopathological pattern in these two species of B. canis.     

The epidemiology of canine Babesia infections in Zambia

This study of 1196 dogs over a period of 18 months determined the seasonal infection patterns of canine babesiosis in Lusaka, the capital city of Zambia. The work also describes a retrospective study of the prevalence of canine babesiosis in laboratory clinical blood samples submitted to the University of Zambia, School of Veterinary Medicine for routine haematological examination from the year 1994 to 2009. A cross-sectional study was also performed to determine the levels of Babesia in a low-income society (during the dry season and the wet season of the year), where 361 samples were collected from dogs presented for mass rabies vaccination campaigns. Morphology of the Babesia indicated that all were of the large-sized Babesia canis infection. Babesia-positive dogs had significantly higher rectal temperatures than negative ones, and dogs younger than 1 year were more likely to be Babesia positive followed by those between 2 and 5 years old. Seasonal trends indicate two peaks, one in the rainy season (November-March) and another in the cold dry season (June/July). Monthly prevalence rates of Babesia ranged from 0% to 2.4% in natural populations and from 0% to 28.6% in laboratory specimens. This study shows that Zambia has lower Babesia prevalence than reported in other African countries.     

Confirmation of occurrence of Babesia canis vogeli in domestic dogs in South Africa

The prevalence of Babesia infections in domestic dogs in South Africa was studied using reverse line blot hybridization and 18S sequence analysis. Babesia canis vogeli was confirmed for the first time in domestic dogs in South Africa. Out of a total of 297 blood samples collected from domestic dogs in Bloemfontein, East London, Johannesburg, Durban and from the Onderstepoort Veterinary Academic Hospital, 31 were positive for Babesia canis rossi, whereas B. c. vogeli was detected in 13 dogs. None of the dogs carried both parasites. The detection of B. c. vogeli has implications with regard to prevalence and varied clinical manifestation of canine babesiosis in South Africa.     

湖南省常德地区宠物犬皮肤病的流行病学调查

为了解湖南省常德地区宠物犬皮肤病流行病学规律,采用回顾性查阅常德地区动物医院的病历、临床诊断及跟踪调查、显微镜检查、数据统计等方法,对该地区宠物犬皮肤病总发病率、皮肤病种类以及皮肤病的发生与宠物犬的种类、性别、年龄、季节的关系进行整理分析。结果表明：常德地区犬皮肤病的发病率为15.9%,其中发病率最高的是贵宾犬,占总皮肤病患犬病例的41.1%;发病年龄以1~3岁的幼犬发病率最高;耳部为易发部位;以混合感染为主;夏季为多发季节。     

477例门诊犬寄生虫种类和感染情况的调查

对2012年-2015年西北农林科技大学西安动物医院门诊确诊的477例犬寄生虫感染资料进行分类统计和分析,以期了解西安地区犬寄生虫的种类和感染情况,对犬寄生虫的防控提供依据。结果显示,从感染犬中共检出14种寄生虫,每种寄生虫的阳性率依次为:吉氏巴贝斯虫51.79%(247/477),等孢球虫16.15%(77/477),贾第鞭毛虫11.74%(56/477),犬弓首蛔虫6.71%(32/477),疥螨4.41%(21/477),蠕形螨4.41%(21/477),犬复孔绦虫3.78%(18/477),犬钩口线虫2.31%(11/477),犬耳痒螨1.89%(9/477),吸吮线虫0.63%(3/477),华支睾吸虫0.42%(2/477),弓形虫0.42%(2/477),犬尾旋线虫0.21%(1/477),犬恶丝虫0.21%(1/477)。混合感染两种及以上寄生虫的犬占5.45%(26/477)。肠道寄生虫中的等孢球虫、贾地鞭毛虫、犬弓首蛔虫和犬钩口线虫等容易发生混合感染。寄生虫和病毒合并感染犬占8.39%(40/477),犬细小病毒(CPV)、犬瘟热病毒(CDV)和犬冠状病毒(CCV)常与犬肠道寄生虫合并感染。许多寄生虫感染与犬的年龄有密切关系,季节对寄生虫感染也有影响。原虫感染特别是吉氏巴贝斯虫感染应当引起重视。控制犬与寄生虫传播媒介的接触、改善犬的饲养管理条件对寄生虫感染的预防十分重要。     

Molecular fluorescent approach to assessing intraerythrocytic hemoprotozoan Babesia canis infection in dogs

The development of recent flow cytometry-based protocols for the diagnosis of canine babesiosis, Babesia gibsoni in particular, has encouraged us to investigate its applicability to detect B. canis-infected erythrocytes as well as optimize the hydroethidine-flow cytometry methodology (HE-FC), using peripheral blood samples from naturally and experimentally infected dogs. Our data demonstrated that HE at 25 microg/ml provided the most outstanding fluorescence profile, able to discriminate between infected and uninfected dogs with no alterations in cell properties such as forward scatter and unspecific fluorescence. The results were expressed as the percentage of positive fluorescent erythrocytes (PPFE) for each individual sample, with 1.53% of PPFE as the cut-off determined between infected and uninfected animals. B. canis-infected erythrocytes during both acute and chronic experimental infection were identified through HE-FC, validating its use for diagnosis purposes in endemic areas for canine babesiosis. In a clinical trial, 22.8% out of 162 dogs showed to be positive to Babesia infection through this approach. Such prevalence was similar to that estimated for altered hematological profiles (HT) < or = 30% (29%), but highly distinct from the prevalence provided by direct blood smear (BS) examination (1.8%) or immunofluorescent assay (IFA) (60.5%). Furthermore, our findings indicate that positive PPFE data was associated with HT < or = 30%, emphasizing that, in clinical practice, the haematocrit should be used as a screening test followed by HE-FC, suitable to confirm hypotheses of canine babesiosis.     

Antibody activity to Babesia canis in dogs in North Carolina

Sera from 600 dogs from 6 geographic regions of North Carolina were analyzed for antibody activity to Babesia canis, using an indirect fluorescent antibody test. Overall, 3.8% of the dogs had an antibody titer greater than or equal to 1:80, which was considered to be indicative of infection. Dogs were more likely to be seropositive if they were housed at humane facilities and were located within areas of the state with a milder climate than were pet animals living within colder regions of North Carolina.     

犬细小病毒病的流行现状调查

对犬细小病毒病流行现状进行调查,为本病的预防、诊断和治疗提供依据。 用犬细小病毒抗原检测试剂盒对2008年门诊的具有腹泻、呕吐、粪便带血等体征的189只患犬作病原检测,对犬细小病毒阳性病例兼有发热、咳嗽体征的16例病犬同时作犬瘟热病毒抗原检测。结果犬细小病毒阳性病例共137只,发病年龄从1个月～15岁各年龄段均有分布,以6月龄以下幼龄动物发病较多,占65.7%。流行季节差异不大,以春季病例略多,占27.0%。犬细小病毒与犬瘟热病毒混合感染的有12例,占8.8%。对在疫苗接种免疫保护期内的4只患犬进行检测,3只犬细小病毒阳性。结论：①免疫力尚未建立是幼犬发病的主要原因;②不按规定的免疫程序对动物进行加强免疫是成年犬发病的主要原因;③在部分发病幼犬中存在犬细小病毒与犬瘟热病毒的混合感染;④老龄动物对免疫的应答能力下降可导致免疫失败。     

潍坊市犬奇异变形杆菌分离鉴定及耐药性检测

奇异变形杆菌为人兽共患病原菌,多为继发感染.本研究从潍坊地区宠物医院的犬瘟热患病犬眼鼻分泌物和犬细小病毒感染患病犬直肠粪便中,共采集样品39份,进行奇异变形杆菌分离鉴定和耐药性检测.结果显示:分离鉴定出11株奇异变形杆菌,分离率为28.20％,其中犬细小病毒感染犬的奇异变形杆菌分离率为47.37％;16S rRNA序列...