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1.
Parisi A Lanzilotta SG Addante N Normanno G Di Modugno G Dambrosio A Montagna CO 《Veterinary research communications》2007,31(1):113-123
Eleven cattle farms, 8 layer farms, 7 broiler farms and 30 broiler meat samples were investigated in south-eastern Italy throughout
2003 to evaluate the prevalence, the molecular type and antimicrobial resistance of thermophilic Campylobacters. A total of
398 samples were analysed. One Campylobacter isolate for each positive faecal swab and three isolates per positive broiler meat sample were selected for further analysis.
Multiplex PCR was performed for species-level identification and PCR-RFLP of the flagellin A gene for genotyping. Resistance
to 14 antimicrobials was studied in 188 Campylobacter isolates. Prevalence of campylobacters was high both on farms (100%) and in food samples (73%). On 4/11 cattle farms and
on 10/15 poultry farms more than one species was isolated. The presence of more than one genotype was found on 8/11 cattle
farms, on 10/15 poultry farms and in 8/22 Campylobacter-positive food samples. High rates of resistance to quinolone were observed: 9/31 (29%) C. jejuni bovine isolates, 4/22 (18%) C. jejuni poultry isolates, and 14/26 (54%) C. coli poultry isolates. Resistance to sulphamethoxazole-trimethoprim was also observed frequently: 18/26 (69%) of the avian C. coli strains, 25/31 (80%) of the C. jejuni strains isolated from poultry and 15/22 (68%) of those isolated from cattle were resistant. There was a significant difference
between the rate of resistance to macrolides of C. coli and C. jejuni isolated in poultry, which amounted to 23% and 3%, respectively. This study provided data on the prevalence and antimicrobial
resistance of thermophilic campylobacters in south-eastern Italy and confirmed that flaA-typing is an efficient tool to study the epidemiology of Campylobacter strains in short-term investigations. 相似文献
2.
Between September and December 1995, 72 out of 476 cattle on 15 dairy farms in the Dar es Salaam region of Tanzania, died of suspected Dichapetalum poisoning. Following a drought and a resultant forage shortage, 12 of the farms had purchased hay from commercial farms in the Coast region, particularly from one seed and hay farm located about 60 km west of Dar es Salaam city. Following ingestion of the purchased hay, affected animals were acutely ill and their clinical signs included inappetence, dullness, high stepping gait, disinclination to move, jugular vein distension and gastrointestinal malfunctions. Fatal cases died suddenly after a short course of illness. Toxic plants identified as Dichapetalum mossambicense Engl. and D. stuhlmannii Engl. were found mixed in the hay. A diagnosis of Dichapetalum poisoning was made on the basis of history, clinical signs and post-mortem findings in fatal cases. On withdrawal of the contaminated hay, the outbreak subsided and deaths ceased. The findings of the investigation are discussed. 相似文献
3.
Risk factors of bovine tuberculosis in cattle in rural livestock production systems of Ethiopia 总被引:2,自引:0,他引:2
Rea Tschopp Esther Schelling Jan Hattendorf Abraham Aseffa Jakob Zinsstag 《Preventive veterinary medicine》2009,89(3-4):205-211
This study shows a representative stratified cluster sample survey of the prevalence of comparative intradermal tuberculin test in cattle from four regions in Ethiopia. Using a cut-off for positivity of 2 mm, it assesses possible risk factors for tuberculin-positive reaction in cattle. Seventy-three villages in 24 kebeles (administrative units) were randomly selected, from which 2216 cattle from 780 owners were tested. In addition, 450 of these cattle owners were interviewed for risk factor assessment. Ninety-nine percent of the tested cattle in this rural livestock production system were traditional zebus. The individual overall prevalence of cattle bovine tuberculosis (BTB)e was 3%, with the highest found in Meskan Mareko, in Central Ethiopia (7.9%) and the lowest in Woldia, in the North East edge of the Rift Valley (1.2%). Generalised Linear Mixed Models (GLMM) with random effect on kebeles was used to analyse risk factors of cattle reactors and human tuberculosis (TB) infection. Purchase of cattle and presence of other livestock in the herd were statistically significant, with OR: 1.7, p-values of 0.03 and OR: 2, p = 0.05, respectively. Family members diagnosed with TB or showing clinical signs of extra-pulmonary TB (EPTB) were reported in 86 households (19%). None of the assessed potential risk factors of disease transmission between cattle and human (food consumption, livestock husbandry and presence of BTB-positive cattle) were statistically significant. 相似文献
4.
Liebana E Johnson L Gough J Durr P Jahans K Clifton-Hadley R Spencer Y Hewinson RG Downs SH 《Veterinary journal (London, England : 1997)》2008,176(3):354-360
The aim of this study was to obtain a contemporary data set of pathology in tuberculin reactor and in-contact cattle in England and Wales. Four hundred animals (200 reactors and 200 in-contacts) from 242 farms located in 14 counties in Western England and Wales were examined. The mean number of lymph nodes (LNs) with tuberculosis (TB)-like lesions per TB-confirmed animal was 1.7 in reactors and 1.5 in in-contact animals. Tuberculous lesions in both reactor and in-contact animals were most commonly observed in the LNs of the thorax, followed by the head and abdomen, particularly the mediastinal, retropharyngeal and tracheobronchial LNs. Twenty-five reactors had macroscopic lesions in the palatine tonsils. Among TB-confirmed cattle, 27% of reactors and 9% of in-contact animals had gross TB-like lesions in the lungs, particularly in the caudal lobes. Gross lesions that were not TB-confirmed were parasitic granulomas (45%), bacterial or mycotic club-forming pyogranulomas (27%) and bacterial abscesses (23%). Diagnostic sensitivity was maximised when bacteriology and histopathology were used concurrently. Stage IV granulomas, alone or in combination with other stages, constituted 63% of lesions, while 16% of lesions were stage I/II granulomas. Caseous necrosis and calcification were common features of the granulomas encountered in natural Mycobacterium bovis infections, even with pathology limited to a small number of sites. Granulomas often covered large areas of histological sections and typically contained only small numbers of acid fast bacilli. 相似文献
5.
A cross-sectional study was conducted between May 2003 and January 2004 on 130 households and 655 (246 indigenous and 409
crossbred) cattle to determine the prevalence of bovine tuberculosis (bTB) and assess risk factors associated with prevalence
in smallholder dairy and traditionally managed herds in the Tanga region of North-eastern Tanzania. Random sampling, single
intradermal tuberculin (SIT), comparative intradermal tuberculin (SCIT) tests and a questionnaire were used to gather individual
animal and herd level information. From 642 animal tested by SIT, 35 (5.4%) were positive reactors for tuberculosis. Out of
those 35 bTB positive reactors, eight (1.25%) proved to be positive reactors for tuberculosis upon further testing by SCIT.
Based on the SCIT test, individual animal prevalences of bTB in the smallholder dairy and traditionally managed cattle was
2% and 0%, respectively. The corresponding overall herd prevalence was 5.7% and 0%, respectively. In conclusion, bTB prevalence
seems low; however, its potential risk to public health is of concern; underscoring the need for further research, active
surveillance to better understand the epidemiology of the disease in different cattle production systems in Tanzania. 相似文献
6.
A 31-year record-based retrospective study was carried out to determine the aetiology and temporal distribution of bovine clinical mastitis in smallholder dairy herds in the Dar es Salaam region of Tanzania over the period November 1971-December 2002. Laboratory information on 1964 quarter samples from 1365 cows in 281 smallholder dairy herds were retrieved, compiled and studied. Eighty-eight percent of the quarter samples were culture-positive and the predominant mastitis pathogens isolated were Staphylococcus aureus (25.7%), Streptococcus agalactiae (15.4%), Klebsiella pneumoniae (14.3%) and Escherichia coli (14.1%). Other isolates included Pseudomonas aeruginosa (7.5%), Streptococcus dysgalactiae (5.2%) and Streptococcus uberis (4.2%). Contagious mastitis pathogens were isolated from 45.6% of the culture-positive samples, whereas environmental and miscellaneous pathogens were isolated from 48.2% and 5.7%, respectively. Thirty percent of the miscellaneous mastitis pathogens were Candida species. The results demonstrate a steady increase in clinical Candida albicans mastitis. The prevalence of Candida albicans has increased from 1% in 1971 to 17.0% in November 2002. Conversely, despite some fluctuations, the prevalence of Staphylococcus aureus, Streptococcus agalactiae, E. coli and K. pneumoniae remain above 10%. The possible risk factors for these observations are discussed. 相似文献
7.
Thekisoe OM Omolo JD Swai ES Hayashida K Zhang J Sugimoto C Inoue N 《The Onderstepoort journal of veterinary research》2007,74(4):339-342
The sensitivity of LAMP, PCR and microscopy to detect Theileria spp. and Trypanosoma congolense in field-derived bovine blood samples from Tanzania was evaluated and compared. No parasites were detected by microscopy. Furthermore, no bovine Theileria spp. were detected by LAMP and PCR from all the 24 samples collected from Arusha. Four and one out of 24 samples were positive for Theileria congolense infection by LAMP and PCR respectively while, 18 and nine out of 40 samples from Dar es Salaam were positive by LAMP and PCR for Theileria spp. Infection, respectively. Although all samples from Dar es Salaam were negative for Trypanosoma congolense infections by PCR, 12 out of 40 samples were LAMP positive. Whilst PCR is an established gene amplification method for the detection of Theileria and trypanosome parasites, this study introduces LAMP as an alternative molecular diagnostic tool that could be used in large-scale epidemiological surveys. 相似文献
8.
Waruiru RM Kyvsgaard NC Thamsborg SM Nansen P Bøgh HO Munyua WK Gathuma JM 《Veterinary research communications》2000,24(1):39-53
A survey of gastrointestinal parasite infections of young (<6 months old), immature (6–12 months old) and adult (>12 months old) dairy cattle on 16 farms in Kiambu District, Kenya was conducted during a dry season (September 1991 to January 1992) and during a wet season (March to July 1992). The survey was based on monthly coproparasitological examination of cohorts and worm counts in tracer calves. The effects of age, sex, farm and season on the prevalence and intensity of helminth and coccidial infections were determined. Faecal egg and oocyst counts revealed that the overall prevalences were: strongyles (including trichostrongyles) (85.5%), liver flukes (Fasciola gigantica) (34.0%), coccidia (30.9%) and tapeworms (9.6%). Eight species of the protozoan Eimeria were identified, the most prevalent species being E. bovis and E. zuernii. The most prevalent nematode genera were Haemonchus, Cooperia, Oesophagostomum and Trichostrongylus. Season, farm and age of the animals had a significant (p<0.05) influence on the intensity of infection with strongyles, liver flukes and coccidia, whereas the sex of the animals had no significant (p>0.05) effect on the prevalence or intensity of infections. A higher intensity of infection with strongyles and coccidia was found in the wet season than in the dry season (p<0.05). The age-specific intensity was in the following order: for strongyles, immature animals of 6–12 months of age had the highest egg counts, followed by young calves and adults. Calves had significantly (p<0.05) higher oocyst counts than immatures or adults. Liver fluke egg counts did not differ significant (p>0.05) between immatures and adult cattle. 相似文献
9.
The prevalence of Taenia saginata cysticercosis in cattle slaughtered for meat in Addis Ababa Abattoir, Ethiopia between September 2004 and August 2005 was
reported. The examination of various organs of 11227 cattle in Addis Ababa Abattoir showed that 842 (7.5%) were infected with
T. saginata cysticercosis. The tongue, masseter muscles, cardiac muscles, triceps muscles and thigh muscles were the main predilection
sites of the cysts. The cysts of bovine cysticercosis were also identified on the spleen, intercostal muscles, diaphragm and
liver. Out of 10329 male cattle, examined, 783 (7.6%) had cysts of bovine cysticercosis while 59 (6.6%) of the 898 female
animals investigated were infected. The animals slaughtered were all adults. No significant difference in prevalence rates
was recorded between the sexes. The prevalence of bovine cysticercosis was higher in local zebu cattle breeds than Holstein-Frisian
cattle. 相似文献
10.
A study was carried out to establish the prevalence of extra-intestinal porcine helminth infections and to assess the pig
slaughter slab sanitary conditions in Dar es Salaam city, Tanzania. A total of 24 privately owned pig slaughter slabs were
assessed. All slaughter slabs were sub-standard; wrongly located, poorly designed and constructed and lacked most basic requirements
for a slaughter house. Because of inadequate slaughtering, disposal and cleaning facilities, the slaughter slabs were under
unhygienic condition with questionable safety, soundness and wholesomeness of the pork produced. Routine meat inspection procedures
were used to detect extra-intestinal porcine helminth infections. Of the 731 examined pigs; 8.1%, 5.9% and 0.4% were infected
with ascariosis, porcine cysticercosis and hydatidosis, respectively. It was noted that almost all slaughter pigs in Dar es
Salaam originated from different regions. Based on the region of origin, the status of porcine cysticercosis was 8.2% for
Dodoma (n = 98), 8.2% for Manyara (n = 260) and 6.9% for Mbeya (n = 116). This study disclosed the unhygienic sanitary condition prevailing in Dar es Salaam pig slaughter slabs and recommends
that strategies should be devised to improve the situation. Porcine ascariosis and cysticercosis were widely prevalent and
caused economic losses due to condemnations. Because of their zoonotic nature, the observed extra-intestinal porcine helminth
infections in pig pose a public health risk among consumers. Thus, there is a need to introduce appropriate control measures
of parasitic infections in pigs. 相似文献
11.
Bovine brucellosis, caused by Brucella
abortus, is a significant problem for both public and animal health in Turkey. This study was conducted on the calving seasons between
2001 and 2006. A total of 626 serum samples of cattle obtained from 27 herds with a history of abortions was examined for
Brucella antibodies by RBPT, SAT and ELISA. Of the cattle sera analysed, 221 (35,30%) and 206 (32, 92%) and 247 (39,45%) were found
to be positive by RBPT , SAT and ELISA, respectively. B. abortus was isolated from 48 (32,21%) of 149 lung samples and stomach contents of the aborted fetuses. Based on the biochemical tests
and the agglutination tests with monospecific A and M antisera, only 3 of the isolates were found to be B. abortus biotype 1 and the remaining 45 were biotype 3. This study also revealed that the dominant biotype of B. abortus was biotype 3 in this region. The determination of the agents responsible for bovine brucellosis and serosurvey of this disease
are expected to help better understanding of this zoonotic infection in this region and neighbouring countries. 相似文献
12.
Mycobacterium farcinogenes is the causal agent of bovine farcy, a chronic infectious disease of zebu cattle in some parts of tropical Africa. Whole
cell homologous antigen of M. farcinogenes was used in the standardization and evaluation of an enzyme linked immunosorbent assay (ELISA) for the detection of circulating
antibodies against bovine farcy using sera from confirmed bovine farcy and from bovine farcy-free cattle. The cut-off optical
density (OD) value was decided at 1.8 using filter 405nm after one hour of incubation at 37°C. Accordingly, 115 out of 124
(92.7%) serum samples from clinically proven bovine farcy cattle were reported sero-positive. Sera from cattle infected with
M. avium and M. paratuberculosis revealed OD value <1.8, indicating the differential diagnostic ability of M. farcinogenes antigen. Our test sensitivity was 92.7% and specificity was 97%, therefore could be routinely employed to support early clinical
diagnosis, epidemiological surveys and for screening animals before exportation to farcy-free regions. 相似文献
13.
A cross-sectional study was conducted to determine three parameters of the quality of the raw milk marketed by milk selling
points (MSPs) in Dar es Salaam region. Total bacterial count (TBC) was used as an indicator of the microbial quality of the
milk; antimicrobial residues were determined; and the California mastitis test (CMT) was used to screen for milk somatic cells
as an indication of the mastitis level in the cows that provided the milk. Moreover, a water sample at each MSP was taken
for bacteriological culturing. Finally, a questionnaire survey was conducted with the milk sellers at the MSPs to identify
risk factors for poor milk hygiene. A total of 128 milk samples and corresponding water samples were collected from randomly
selected milk selling points in Dar es Salaam region. The mean TBC was (8.2± 1.9) × 106 cfu/ml, and major bacterial isolates from the milk samples were Escherichia coli (6.3%), Bacillus cereus (6.3%), Staphylococcus aureus (6.3%) and Streptococcus agalactiae (6.3%), Enterobacter aerogenes (5.6%) and Enterococcus faecalis (4.7%). In most cases, the organisms identified in milk corresponded to those isolated from the corresponding water samples.
Of milk samples, 79.0% were positive to the CMT and 7.0% were positive for antimicrobial residues. TBC was normalized by log-transformation,
and the possible predictors of TBC were identified by fitting two linear regression models. In a random effect model, water
microbial quality, frequency of cleaning the milk containers, frequency of milk supply, milk storage time and the type of
containers, and mixing of fresh and previous milk were significantly (p < 0.05) associated with the mean log TBC. In a fixed effect model, in addition to these indicators, water shortage, water
source and the refrigerator condition were significantly (p ≤ 0.01) associated with log TBC. It was concluded that the milk sold in Dar es Salaam region is of poor quality and is of
public health significance. 相似文献
14.
Bovine tuberculosis (bTB) is a continuing problem in British herds. Micro-nutrients are important for the maintenance of well-functioning immune system. The aim of this study was to determine whether the selenium, copper and vitamin B12 status of cattle was associated with Mycobacterium bovis (M. bovis) infection. Between 2002 and 2005, 200 cattle (43% dairy, mean age 4.6 years), reactors according to the standard interpretation of the tuberculin test, and 200 in-contacts (41% dairy, mean age 4.4 years) non-reactors, which had been in contact with cattle with bTB, were selected from herds in England and Wales. Levels of the seleno enzyme glutathione peroxidase (GSHPx), copper and vitamin B12 were measured in blood. Confirmation of bTB infection was made by bacteriological culture and histopathology following a detailed postmortem. Levels of selenium and copper were also measured in a random sample of 63 livers. bTB was confirmed by culture/histology in 23/200 (11.5%) of in-contacts and 110/200 (55%) of reactors. In blood drawn at recruitment, GSHPx was lower in cattle with confirmed bTB compared to other cattle (geometric means 59.7 u/mL versus 78.9 u/mL red blood cells (RBC), p < 0.01). Vitamin B12 was similar (geometric means 161.5 pmol/L versus 165.5 pmol/L, p = 0.62) and copper was similar (geometric means 14.4 μmol/L versus 14.1 μmol/L, p = 0.55). In logistic regression models including all micro-nutrients simultaneously and controlling for age, sex, animal production class, herd size, number of reactors, postmortem laboratory and seasonal trends, lower levels of GSHPx (adjusted OR 0.42, 95% CI 0.21–0.81 per 100 u/mL RBC, p = 0.01) and higher levels of copper (adjusted OR 1.69, 95% CI 1.21–2.36 per 5 μmol/L, p < 0.01) were associated with an increased risk of confirmed bTB but there was no association with vitamin B12. There was evidence for a stronger association between confirmed bTB and GSHPx in in-contacts (adjusted OR 0.21, 95% CI 0.06–0.79 per 100 u/mL RBC) compared to reactors (adjusted OR 0.50, 95% CI 0.21–1.23 per 100 u/mL RBC) (p = 0.08 for interaction). Lower liver copper was associated with a higher risk of confirmed bTB (adjusted OR 0.15, 95% CI 0.02–1.0 per 5000 μmol/kg dry mass, p = 0.05) but there was no association between liver selenium and bTB. Trace micro-nutrient status may affect susceptibility to M. bovis infection in cattle. Further studies are needed. 相似文献
15.
16.
Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran 总被引:1,自引:0,他引:1
Akbar Keshavarz Ali Haghighi Amid Athari Bahram Kazemi Alireza Abadi Ehsan Nazemalhosseini Mojarad 《Veterinary parasitology》2009,160(3-4):316-318
The aim of this study was to determine the prevalence, variability with host age, and the genotypes of species of Cryptosporidium in cattle from 15 dairy farms in Qazvin province, Iran. Fecal samples, collected from 272 cattle during May 2006 to December 2007, were characterized microscopically. Oocysts from 51 positive samples were analyzed using PCR assay of 18S SSU rRNA, restriction fragment length polymorphism (RFLP) and sequencing. We identified 72.6% of the positive samples as Cryptosporidium parvum, 17.7% as Cryptosporidium andersoni, 7.8% as Cryptosporidium bovis and 1.9% as a novel genotype of C. parvum possessing a single mutation on MboII restriction. An infection rate of 19.5% of C. parvum among 174 pre-weaned calves was significantly higher than the 3.1% among 98 post-weaned calves (P < 0.0006). This is the first report of C. bovis and the new subgenotype of C. parvum in Iranian cattle. 相似文献
17.
18.
Waruiru RM Thamsborg SM Nansen P Kyvsgaard NC Bogh HO Munyua WK Gathuma JM 《Tropical animal health and production》2001,33(3):173-187
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round. 相似文献
19.
Duong MC Alenius S Huong LT Björkman C 《Veterinary journal (London, England : 1997)》2008,175(3):390-394
The main purpose of this study was to investigate the prevalence of Neospora caninum and bovine viral diarrhoea virus (BVDV) in some dairy herds in Southern Vietnam, and to ascertain whether there were differences in seroprevalences between herds with imported and locally bred cows. Serum samples collected on five state farms and 97 smallholder herds were analysed for the presence of antibodies to N. caninum and BVDV. All BVDV antibody-negative sera were further tested by antigen-ELISA in order to identify persistently infected individuals. The N. caninum prevalence varied between 16% and 53% in the state herds, and was higher in the four herds that had imported cows than in the herd that only had locally bred cows. Nineteen percent of the samples collected on smallholder farms, which all had only locally bred cows, had antibodies to N. caninum. The BVDV seroprevalence varied between 58% and 93% on the state farms. In smallholder herds, the prevalence of BVDV among the sampled cows was 18% and even lower on the state farms. Despite the high seroprevalence for BVDV in the state herds, no persistently BVDV infected cows were found. Given the high prevalence for Neospora and BVDV among herds with imported cows, it seems advisable to test for both infections before cattle are imported into the country. 相似文献
20.
2021年—2022年云南省寻甸县肉牛科技特派团成员对寻甸县肉牛主要疫病进行系统调查及疫病防治研究,2年来共完成了16个乡(镇)175个肉牛养殖场(户)的现场调查,并随机在全县16个乡镇71个肉牛养殖场(户)、1个屠宰场和1个大牲畜交易市场采集样品3267份,进行了口蹄疫、牛结节性皮肤病、牛蓝舌病、巴氏杆菌病、牛支原体、牛结核分枝杆菌、牛病毒性腹泻、牛传染性鼻气管炎、牛布鲁氏菌病、巴贝斯虫10个病的病原学检测,结果显示:牛结节性皮肤病、蓝舌病病毒、牛巴氏杆菌病、牛支原体、牛结核病、巴贝斯虫病6个病种的病原监测均为阴性;在73个养殖场(户)1394份棉拭子中检出牛病毒性腹泻阳性3份、牛传染性鼻气管炎阳性2份;在29个养殖场(户)899份全血样品中检出牛巴贝斯虫病12份,样品阳性率分别为0.22%,0.14%,1.33%;场阳性率分别为1.37%、1.37%,24.13%。通过2年持续地边监测边排查隐患,精准指导养牛户科学防控疫病、规范使用兽药、强化生物安全等措施,使养殖户掌握了科学养牛和科学防病技术,切实提高肉牛疫病防控和养殖水平,提高肉牛养殖效率和肉牛产品品质。 相似文献