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1.
The effect of 1-methylcyclopropene (1-MCP) on shelf-life and postharvest quality of sweet basil detached leaves was examined. Treatment with 0.2, 0.4 and 0.6 g m−3 1-MCP for 8 h was conducted at 15 °C in the dark. After the treatment the leaves were packed in polyethylene bags then sealed and stored at 20 °C. All 1-MCP concentrations significantly increased the shelf-life of leaves compared to the untreated control, and leaf weight loss with 1-MCP treatment was minimal. 1-MCP treatment significantly retarded the degradation of chlorophyll and protein content of detached leaves during storage and decreased leaf ethylene production. 1-MCP treatment also significantly retarded the decrease of volatile oil percentage in detached leaves during storage compared to the control. Among 1-MCP concentrations, 0.4 g m−3 resulted in the maximum shelf-life as well as improved postharvest quality of the leaves. The results clearly indicate that a single treatment with 1-MCP may provide a feasible technique for extending the shelf-life and maintaining higher volatile oil percentage of sweet basil leaves.  相似文献   

2.
Our previous studies demonstrated that tomato fruit (breaker or pink) exposed at the midclimacteric stage to hypobaric hypoxia for 6 h exhibited transient increased sensitivity to subsaturating levels of 1-methylcyclopene (1-MCP). In the present study, we examined the effect of gaseous 1-MCP (500 nL L−1, 20.8 μmol m−3) applied to mid-climacteric (>60% peak ethylene production) tomato fruit under hypobaric hypoxia (10 kPa, 2.1 kPa O2,) for 1 h. Application of 500 nL L−1 1-MCP under atmospheric conditions had little effect on softening and timing and magnitude of peak ethylene production, and moderate effects on respiration and lycopene and PG accumulation. By contrast, midclimacteric fruit exposed to 500 nL L−1 gaseous 1-MCP under hypobaric hypoxia for 1 h showed acute disturbance of ripening. Firmness and hue angle declines were delayed for ten days and peak ethylene production for eleven days compared with trends for the other treatments. Maximum ethylene production did not exceed 50% of maxima for the other treatments and a definitive respiratory climacteric was not observed. Accumulation of internal gaseous 1-MCP was enhanced under hypobaric hypoxia. Internal 1-MCP in fruit exposed to 20 μL L−1 1-MCP (831 μmol m−3) under hypobaric hypoxia for 2 or 10 min averaged 7.5 ± 0.5 and 8.7 ± 1.4 μL L−1, respectively, compared with 0.8 ± 0.3 and 3.9 ± 0.7 μL L−1 in fruit exposed under atmospheric conditions. After 1 h exposure, internal 1-MCP averaged 10.8 ± 2.2 μL L−1 under hypobaric hypoxia compared with 5.3 ± 1.4 μL L−1 under atmospheric conditions. The results indicate that high efficacy of 1-MCP applied under hypobaric hypoxia is due to rapid ingress and accumulation of internal gaseous 1-MCP.  相似文献   

3.
The storability of onion bulbs is dependent on the incidence and rate of sprout growth. Exogenous ethylene applied continuously has been demonstrated to act as a sprout suppressant in onion. However, the ethylene binding inhibitor, 1-methylcyclopropene (1-MCP), can also suppress sprouting in onion. Given this seemingly contradictory result, the precise role that ethylene plays during onion storage and the effect of curing on its efficacy is not understood.‘Sherpa’ and ‘Wellington’ onion bulbs were treated before or after curing (28 °C for 6 weeks) with a single dose of 10 μL L−1 ethylene or 1 μL L−1 1-MCP for 24 h at 20 °C, or no treatment (control). Replicated out-turns were sampled during 38 weeks storage at 0–1 °C. Sprout growth (31 weeks after harvest) was reduced in ‘Sherpa’ treated before curing with ethylene or before or after curing with 1-MCP. However, sprout growth of ‘Wellington’ was not affected by any treatment. Following treatment, the cured, thick-skinned ‘Wellington’ released a lower concentration of treatment gas compared with the newly harvested, thin-skinned ‘Sherpa’. Onion bulb respiration rate increased immediately after being treated with ethylene but to a lesser extent or not at all when treated with 1-MCP. Fructose concentrations of onions treated with ethylene or 1-MCP before curing were not significantly different, however, after curing concentrations were about 2-fold higher compared with the control. Mean glucose and sucrose concentrations for both cultivars were higher immediately after being treated before curing with ethylene or 1-MCP than control bulbs. It appears that inhibition of sprout growth can be achieved using just a short 24 h treatment with ethylene or 1-MCP. However, skin thickness or permeability, which is dependent on cultivar and curing, may affect ethylene or 1-MCP influx and therefore efficacy of sprout suppressant action.  相似文献   

4.
The influence of aqueous 1-methylcyclopropene (1-MCP) concentration, immersion duration, and solution longevity on the ripening of early ripening-stage tomato (Solanum lycopersicum L.) has been investigated. Tomato fruit at the breaker-turning stage were fully immersed in aqueous 1-MCP at 50, 200, 400 and 600 μg L−1 for 1 min, quickly dried, and then stored at 20 °C. Ethylene production, respiration, surface color development, and rate of accumulation of lycopene and polygalacturonase (PG) activity were suppressed and/or delayed in fruit exposed to aqueous 1-MCP. Suppression of ripening was concentration dependent, with maximum inhibition in response to 1 min immersion occurring at concentrations of 400 and 600 μg L−1. Climacteric ethylene peaks were delayed approximately 6, 7, and 9 d and respiration was strongly suppressed in fruit treated with aqueous 1-MCP at 200, 400, and 600 μg L−1, respectively, compared with control fruit. Fruit firmness, lycopene content, PG activity, and surface hue of fruit treated at the three higher levels remained strongly suppressed compared with control. Skin hue values and pericarp lycopene content in response to treatment at the subthreshold 50 μg L−1 provided evidence for differential ripening suppression in external versus internal tissues. Maximum delay of softening and surface color development in response to 50 μg L−1 aqueous 1-MCP occurred following immersion periods of between 6 and 12 min. Factors affecting fruit penetration by aqueous 1-MCP and mechanisms contributing to recovery from 1-MCP-induced ripening inhibition are discussed.  相似文献   

5.
M. Hatano    R. Nakai    F. Kawanishi  K. Kedo  Y. Shoyama 《Plant Breeding》1997,116(6):589-591
Shoot formation was induced from the tip tissue of Rehmannia glutinosa f. hueichingensis on hormone-free Murashige-Skoog medium within 4 weeks of culture. Shoots were propagated on Murashige-Skoog medium supplemented with l.0mg/l benzy0ladenine for 4 weeks. Propagated shoots rooted on the hormone-free Murashige-Skoog medium during 4 weeks of culture. Total DNA was extracted from the leaves of a F1 hybrid and its parents. R. glulinosa f. huekliingensis and R. glutinosa var, purpurea. Analysis of random-amplified polymorphic DNA (RAPD) using 10 arbitrary oligonucleotide 10-mers. showed the genetic homogeneity ofthe above three species. The F1 hybrid was genetically intermediate between both parental plants, compared with the genetic distance between the F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between the F1 hybrid, obtained from the crossing clearly showed that parts of the bands of both parents. R. glutinosa f. hueichingensis and R. glutinosa var. purpurea, were introduced into the F1 hybrid.  相似文献   

6.
Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F2 plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F2 population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis. The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed in the analysis with several melon cultivars and lines.  相似文献   

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