Utilization of polar metabolite profiling in the comparison of juvenile wood and compression wood in loblolly pine (Pinus taeda)

Juvenile wood (JW) of conifers is often associated with compression wood (CW), with which it is sometimes believed to be identical. To determine whether JW and CW can be distinguished metabolically, we compared gas chromatographic profiles of 25 polar metabolites from rooted cuttings of a single loblolly pine (Pinus taeda L.) clone raised in controlled environment chambers and subject to three treatments: (1) grown erect with minimal wind sway (control); (2) swayed by wind from oscillating fans; and (3) with 30-cm growth increments successively bent at an angle of 45 degrees to the vertical. Profiles were compared by principal component analysis. Substantial increases in abundances of coniferin and p-glucocoumaryl alcohol separated immature JW-forming xylem tissues of the control trees from the CW-forming xylem of the bent and swayed trees.     

Seasonal variation in gene expression for loblolly pines (Pinus taeda) from different geographical regions

In developing xylem, gene expression levels vary in different genotypes, at different stages of development, throughout a growing season, and in response to stresses. Commercially important characteristics such as wood-specific gravity are known to differ with seed source. For example, when grown on a common site, the specific gravity of Arkansas loblolly pine (Pinus taeda L.) trees is greater than that of Louisiana loblolly pine, and Texas loblolly pines have a greater specific gravity than loblolly pines from the Atlantic coast. A microarray analysis was performed to examine variation in gene expression among trees from different geographical sources when grown on a common site, and seasonal variation in gene expression in each seed source. We used microarrays containing 2171 expressed sequence tags (ESTs) with putative functions of interest, selected from several loblolly pine xylem partial cDNA libraries and a shoot tip library. Genes with significant variation in expression for each factor were identified. Many genes preferentially expressed in latewood compared with earlywood were for proteins involved in cell wall biosynthesis. Variation in gene expression among trees from the two seed sources in each growing season suggests that there may be more differences between South Arkansas trees and South Louisiana trees in latewood than in earlywood. Variation in gene expression among trees from different regions may reflect adaptation to different environments.     

湿地松,火炬松木材密度变异和优树选择的研究

研究了影响湿地松、火炬松木材密度变异的主要因素；探讨了对木材密度进行综合选择的选优方法，选出湿地松，火炬松优良单株７３株。     

Proteomic analysis of the G-layer in poplar tension wood

Angiosperm trees bend their stems by forming tension wood at the upper side of leaning stems. Most tension wood has a cellulose-rich G-layer in the innermost surface of the fiber cell wall. Strong tensile stress is considered to occur in the G-layer. This study undertook to identify the proteins involved in G-layer formation and function through a proteomic analysis of G-layer-localized protein. G-layers of poplar were loosened by sonication and isolated as doughnut-shaped pieces of thinly sliced transverse sections. The proteins, once extracted with urea/detergent solution, were separated by two-dimensional polyacrylamide gel electrophoresis, and 110 spots were subjected to liquid chromatography tandem mass spectrometry (LC/MS/MS). A database search for these spots’ mass spectrum patterns identified 72 proteins. In addition, all peptide digestion mixtures of G-layer proteins were separated by strong cation exchange chromatography and 39 proteins were identified using LC/MS/MS analysis. Proteins involved in wall formation, such as lignin biosynthesis-related protein, xyloglucan endotransglucosylase, and fasciclin-like arabinogalactan protein, were notably detected in the G-layer.     

不同种源火炬松和湿地松木材基本密度和管胞长度的变异

对1984年种植在广西和浙江的22个种源的火炬松及在浙江的8个种源的湿地松的木材密度和管胞长度进行了测定和分析。结果表明,两个树种的木材密度和管胞长度在种源间和种源内单株间均表现出显著差异,但株间差异较种源间差异显著得多。不同地区火炬松的差异较之同一地区不同种源间的差异更为显著。种源、地区的交互作用对火炬松的木材密度和管胞长度都表现出显著影响,种源和单株的交互作用对两个树种的木材密度也有显著影响。两个树种的木材材性与年轮宽度之间没有显著的相关关系。与马尾松和早期引种的火炬松、湿地松比较表明,用新引进的材料可选育出木材材质更为优良的品种。     

增加林木的纤维素生产和转基因树的生长(英文)

纤维素是植物组成中的重要多聚物。纤维素也是重要的工业原料和可再生的能源物质。利用转基因技术可以降低林木中木质素含量并增加纤维素含量。木质素合成酶基因4-CL是一个重要的和木材再生有关的基因。在我们的研究中,将利用反义表达方法降低木质素含量,增加纤维素含量。研究包括:D4-CL基因的分离;转基因植物的生产;纤维素和木质素含量分析;中试。参69。     

Ice Damage in a Chronosequence of Agroforestry Pine Plantations in Arkansas,USA

Abstract

Acute (broken and leaning) and transient (bending) damage to loblolly pine (Pinus taedaL.) were assessed in a case study of experimental agroforestry plantations following a December 2000 ice storm. Stand ages were 7-, 9-, and 17-years-old and tree density ranged from 150 to 3,360 trees ha^?1 in rectangular and multi-row configurations. Wider tree spacing or lower stand density of 7-year-old trees increased stem breakage, while closer spacing increased bending. There was substantial straightening of bent 7-year-old trees 8 months after the storm, and this recovery was determined more by degree of initial bend rather than height or diameter. Nine-year-old loblolly pine had 19% more top breakage and 59% more stem breakage than shortleaf pine (P. echinataMill.) (P < 0.001). Agroforestry design influenced ice damage in 7-year-old stands, but no design had catastrophic loss. Thinning from above caused an increased susceptibility of ice damage to a 17-year-old stand compared to a nonthinned stand. The case study supports the cultivation of loblolly pine in areas prone to ice damage.     

Growth of young loblolly pine trees following pruning

Pruning loblolly pine trees is sometimes practiced to improve wood quality even though reduced growth following treatment may occur. Two experiments were established in February 2000 in the Piedmont region of Virginia, USA, to examine the impact of timing and intensity of pruning on subsequent growth of young loblolly pine trees. Results of one study indicated that there is a window of opportunity during the early portion of stand development where up to 50% of the live crown length can be removed without a significant loss of long-term height or diameter growth. Within a year following pruning at ages 3, 6 and 9 (all pruning treatments occurred prior to crown closure), crown mass had been restored and growth comparable to an unpruned control resumed. By age 11 there were no significant differences in cumulative height or dbh of any of the one-lift pruning treatments and the control. Findings from a second study planted at closer spacings where pruning treatments occurred at crown closure (age 6) showed that pruning some of the trees in a loblolly pine plantation does not result in a loss of long-term height or diameter growth or crown dominance for the pruned trees as compared to their unpruned neighbors. For both studies, growth reductions following pruning were small and transitory.     

基于纳米压痕技术的木材胶合界面力学行为

【目的】研究木材胶合界面的静态和动态力学行为,探讨树脂渗透对木材管胞壁层力学性能的影响,为木质复合材料制造工艺优化和增强改性提供理论依据。【方法】采用纳米压痕静态和动态力学测试技术(Nano-DMA),对针叶材火炬松与酚醛树脂(PF)、脲醛树脂(UF)胶黏剂所形成胶合界面区域各相材料的静态弹性模量、硬度、蠕变性能以及储能模量和损耗模量等力学行为进行分析。【结果】静态力学行为方面,在界面区域,PF和UF渗透进入管胞壁层后,木材管胞壁的弹性模量( E r)和硬度( H )提高;经PF渗透后,木材管胞壁的 E r和 H 分别增加7%和26%;Burgers蠕变力学模型可有效描述胶合界面区域管胞壁的纳米压痕蠕变特性,经树脂渗透后,木材管胞壁的瞬时弹性模量增加,黏弹性模量和黏性系数减小;在保载初期,PF界面区域木材管胞壁的蠕变柔量约下降60%,UF界面区域木材管胞壁的蠕变柔量约下降58%。动态力学行为方面,随着加载频率增加,界面材料的储能模量( E ′ r)逐渐增大,而损耗模量( E ″ r)和损耗因子(tan δ)呈减小趋势;当加载频率为10 Hz时,PF和UF树脂渗透使得管胞壁层的储能模量分别增加16%和29%。【结论】胶合界面区域胶黏剂进入管胞壁层,对木材管胞的静态力学性能具有增强作用,同时胶黏剂可提高管胞壁的短期抗蠕变能力;木材管胞壁具有较高的储能模量和损耗模量,而树脂的储能模量和损耗模量较低,经树脂渗透后,木材管胞壁的储能模量增加,但损耗模量和损耗因子呈下降趋势,可能对界面传递和分散应力产生不利影响。     

Use of near infrared spectroscopy to measure the chemical and mechanical properties of solid wood

Near infrared (NIR) spectroscopy (500 nm–2400 nm), coupled with multivariate analytic (MVA) statistical techniques, have been used to predict the chemical and mechanical properties of solid loblolly pine wood. The samples were selected from different radial locations and heights of three loblolly pine trees grown in Arkansas. The chemical composition and mechanical properties were measured with traditional wet chemical techniques and three point bending tests, respectively. The microfibril angle was measured with x-ray scattering. These chemical and mechanical properties were correlated with the NIR spectra using projection to latent structures (PLS) models. The correlations were very strong, with the correlation coefficients generally above 0.80. The mechanical properties could also be predicted using a reduced spectral range (650 nm–1150 nm) that should allow for field measurements of these properties using handheld NIR spectrometers.     

松材线虫病病原与致病机理研究进展

松树是我国重要的造林树种,而松材线虫病是松树的一种毁灭性病害。近年试验表明,松材线虫病病原是松材线虫与伴生细菌。组织病理学研究看出,树脂道泌脂细胞最先受到线虫侵袭,薄壁细胞的死亡是寄主最初的组织病理学反应。松苗皮层薄壁细胞发生程序性死亡,表现出核染色质浓缩,核变形,降解,解体等细胞凋亡。纤维素酶作为松材线虫的致病物质参与早期寄主发病过程.线虫伴生细菌产生活性毒素。管胞空穴化造成输水堵塞致使受害松树死亡。松材线虫携带的细菌在致病过程中起到至关重要作用。可以认为,松材线虫病是由松材线虫和伴生细菌共同侵染引起的复合侵染病害。     

Stand-level allometry in Pinus taeda as affected by irrigation and fertilization

Changing environmental conditions have the potential to alter allometric relationships between plant parts, possibly leading to ecosystem-level feedbacks. We quantified allometric shifts in field-grown loblolly pine (Pinus taeda L.) in response to altered resource availability based on data from multiple harvests to correct for size-related changes in biomass partitioning. A replicated factorial arrangement of irrigation and fertilization treatments was applied for 4 years to an 8-year-old loblolly pine plantation on a well-drained, low fertility site in North Carolina. Destructive and nondestructive growth measurements were used to develop treatment-specific regressions to estimate stand-level biomass for ephemeral and perennial plant parts, both above- and belowground. Stand-level allometric analysis indicated that irrigation increased biomass partitioning to fine roots and decreased partitioning to foliage, relative to other plant parts. Fertilization increased partitioning to perennial tissues (coarse roots, taproots, and branches) and decreased partitioning to ephemeral tissues (foliage and fine roots). Changes in allometry were small (< 6 %) but statistically significant, indicating that biomass partitioning in loblolly pine changes with altered resource availability, but is probably under strong ontogenetic control.     

Preliminary Studies on Establishment of Genetic Transformation System in Loblolly Pine

Introducti0nt,ollilcrsareec0n0l11icall}impo11a11tl0resttree\`orlLl-tvide.beingusedtbrs0iIc0nservatio11-el1vir()11111e11lprotectiol1,\`ood.prodtlctiOI1.andasavariet}ofhIIiId-ingandil1dLlstr}'Inaterial.ForcOI1itbrgel1cticiI11-provel11el1tplan-classicalbreed…     

Cell wall fracture properties in relation to lignin distribution and cell dimensions among three genetic groups of radiata pine

Summary The position of fracture within the cell wall on split tangential longitudinal surfaces of air dry wood was quantified for three genetic groups of Pinus radiata D. Don. Differences in the position of fracture within the cell wall among these groups of trees were compared with lignin distribution and cell wall dimensions. In the control trees equivalent to typical New Zealand grown radiata pine, fracture occurred predominantly between the middle lamella and S1 layers as well as within the S1 layer, producing large numbers of fines on the fracture surface. In the open pollinated NZ850–55 group, fracture occurred predominantly between the S1 and S2 layers as well as within the S1 layer, producing fewer fines on the surface. In the NZ850–55 x Guadalupe group, fracture occurred in a similar fashion to the open pollinated group except for a greater number of transwall fractures exposing the cell lumen on the fracture surface. The differences in fracture behaviour between the control and genetically select groups are attributed to reduced lignification at the S1/S2 boundary in the genetically select trees. Observed differences in both the type of fracture and its location were unrelated to cell dimensions. These observations are discussed in relation to the observed differences in thermomechanical pulping properties exhibited by these groups of trees.