Component yields and quality of binary mixtures of lucerne and perennial, Italian or short rotation hybrid ryegrass

Studies were conducted to characterize sward dynamics of lucerne ( Medicago sativa L.) and perennial, Italian or short rotation hybrid rye-grass ( Lolium perenne L. and L. multiflorum Lam.) binary mixtures under hay management. Lucerne/timothy ( Phleum pratense L.) mixtures were included as controls. The study was conducted on two farms in central Pennsylvania, USA on Hagerstown silt loams (fine, mixed, mesic hapludalf). Total dry matter yield, grass yield and yield of digestible dry matter were higher for tetraploid perennial and tetraploid Italian ryegrass mixtures than for timothy mixtures. Seasonal production differed for the three kinds of ryegrass. Italian ryegrass dry matter yields were higher in cuts 2 and 3 than perennial ryegrass yields. Grass yields represented 34%, 24% and 12% of total yields of the Italian, perennial or short rotation, and timothy mixtures, respectively. However, herbage in vitro dry matter digestibility of Italian was lower than that of perennial ryegrass.
The tetraploid perennial ryegrasses Grimalda and Reveille had high-ranking N concentrations more frequently than other cultivars, while Bar-vestra and Reveille tetraploid perennials and Bison short rotation ryegrass had high-ranking dry matter digestibility values more frequently than the other cultivars. Perennial ryegrass cultivars and Lipo tetraploid Italian ryegrass persisted well for 4 years and produced higher yields with a lower weed content than timothy mixtures. It is concluded that perennial ryegrasses and Lipo Italian ryegrass should be recommended over timothy, as companion grasses with lucerne.     

The effect of autumn fungicide application on spring dry matter accumulation in perennial ryegrass cv. S24

Fungicide applied to plots of perennial ryegrass cv. S24 in the autumn of 1981 significantly increased tiller number and total dry matter accumulation in February and March of 1982. However, fungicide application in the autumn of 1982 failed to increase tiller number or dry matter accumulation in the spring of 1983. It is suggested that fungicide application in autumn 1981 prevented the death of tillers caused by fungal attack after snow covered the plots in December 1981.     

THE PRODUCTION OF EARLY SPRING GRASS

The effects of autumn management and nitrogenous manuring on the production of early spring grass were studied from 1954 to 1957 on farms throughout England and Wales.
The most important management factor influencing spring yields was the application of spring nitrogen. In the early spring 6 to 10 lb. of dry matter were produced per lb. of nitrogen applied. Autumn nitrogen increased herbage yields in the auttimn by 8 to 17 lb. of dry matter per lb. of nitrogen applied.
Italian and perennial ryegrass responded well to autumn nitrogen but it was important to graze off the extra growth fairly quickly to prevent the sward becoming too open. The yield of ryegrass swards which received spring nitrogen were very much higher if they were grazed during the previous September and October than if they had been rested during those months.
In two years neither the autumn management nor alumni fertilizer appeared to influence the spring yields of cocksfoot, timothy or meadow fescue swards, in one year, however, when autumn growth was poor, autumn nitrogen and resting resulted in slightly better yields on these swards in the following spring.
It is suggested that resting swards during September and October to build up "reserves" is relatively unimportant in relation to the yields of grass in the following spring.     

Progress in breeding interspecific hybrid ryegrasses

Stable hybrids were produced between tetraploid perennial and Italian ryegrasses derived from commercial varieties and germplasm introduced from European collections. Spaced plant characteristics were used to select Fl plants for the production of F2 families which were assessed in small plots for growth and quality traits. Ten of the 19 selected hybrid families were based on perennial ryegrass collected from the Zurich Uplands. The influence of this parental material in improving vegetative growth during early spring, late summer and autumn was demonstrated. Its use in improving feeding value by increasing the water-soluble carbohydrate content of hybrids was also shown. Consequently, selected hybrid varieties based on this new genetic resource showed considerable improvements in seasonal growth and quality over the older hybrid variety Augusta and Italian ryegrass variety RvP.     

Effect of combined insecticide and fungicide treatments on newly sown swards of Italian and perennial ryegrass using two methods of sowing, two rates of seed and N fertilizer, with and without herbicide

The effects of six factors, each at two levels and in all combinations, on herbage yield and weed content of perennial ( Lolium perenne ) and Italian ( L. multiflorum ) ryegrass were assessed in four autumn sowings of field plots, in 3 years and at two sites. The factors were (i) perennial (PRG) or Italian (IRG), (ii) normal or low seed rate, (iii) drilling or broadcasting the seed, (iv) normal or low N fertilizer rate, (v) ryegrass fungicide seed treatment and post-emergence insecticide sprays (F + I) or untreated, and (vi) herbicide treatment or untreated. Overall, herbage yield at the first harvest was increased by F + I and IRG (all sowings), and the higher rates of seed (three sowings) and N (two sowings). Variable results were obtained for the other two factors. Increased yield was often associated with reduced weed content. Significant interactions between factors were obtained at two sowings; in particular, F + I increased yield of IRG by 12% and 22%, but had no effect on PRG. A second harvest, in the following spring, was taken for two sowings and there was no effect of F + I or the higher rates of seed and N. Thus, it is concluded that the desired aim when establishing grass swards of an adequate plant population without excessive competition from weeds appeared to be obtained even with plots receiving the least inputs.     

Effect of ryegrass endophyte in mixed swards of perennial ryegrass and white clover under two levels of irrigation and pesticide treatment

In a small-plot experiment at Hurley, UK, infection of perennial ryegrass with the endophytic fungus Acremonium lolii had no apparent effect on seedling populations and herbage yield of ryegrass and white clover sown in mixture. The plots were maintained under two levels of irrigation with or without pesticide (carbosulfan granules) to study possible interactions with stress due to drought or pest damage. The higher level of irrigation consistently increased yield of both grass and clover during 1989 and 1990, but the effects of endophyte and pesticide on yield were not consistent, nor were the few significant interactions between factors. It is concluded that infection of ryegrass does not appear to increase ryegrass production or to decrease white clover production in the UK, in contrast to reports from New Zealand.     

The effect of height and frequency of mowing on the yield and composition of perennial ryegrass—white clover swards in the autumn to spring period

Ninety-six plots (3 × 2 m) of well-established perennial rye grass/white clover pasture were mown to heights of 2·7 (Low) or 3·96 (High) cm (rising plate meter) at 14-, 28-, 84- or 112-d intervals in autumn-winter. A 7-, 14- and 28-d mowing interval was superimposed in spring on each autumn–winter mowing interval treatment with the low and high mowing heights altered to 2·92 and 4·80 cm, respectively.
With the low cutting height, accumulated herbage DM was more than doubled (1806 ± 79 kg DM ha^-1) compared to a 'high' (754 ± 49 kg DM ha^-1) cutting height in autumn–winter and this was due to increased harvesting efficiency rather than growth as estimated by leaf extension. Although defoliation interval had no effect on DM yield, the grass component increased and clover decreased. The composition effect carried over into spring. On average, 3·5 tillers were produced over winter for each ryegrass tiller present in autumn and tiller densities were higher in spring. Tillers produced over autumn–winter contributed more than 60% of ryegrass growth by early spring.
In early spring (16–30 September), the low cutting height increased herbage DM yield, in mid-spring (1–14 October) it reduced DM yields particularly in combination with short defoliation intervals, while in late spring (14 October to 11 November) cutting height had no effect on DM yields.
Over the entire spring period there was a very marked effect of defoliation interval on DM yields.     

The relationships between stolon characteristics, winter survival and annual yields in white clover (Trifolium repens L.)

Five white clover populations of Swiss origin and three bred varieties were grown in binary mixtures with two perennial ryegrass varieties, Aurora and S23. The seasonal yields of clover and grass plus clover were measured under a cutting regime during the second and third years after establishment. A series of destructive detailed sward measurements was made during the late autumn to spring period preceding each harvest year. In this way changes in the amounts of stolon, leaf plus petiole and numbers of growing points were monitored during the winter.
There were large differences in clover yield between populations in both years. These were evident from the first (spring) harvest in each year. Higher-yielding clovers in spring tended to produce higher annual clover yields. No grass × clover interaction was evident at any harvest. Large differences between clovers were also apparent in the morphological characteristics measured, with the Swiss material generally having greater amounts of stolon, leaf plus petiole and numbers of growing points present in early spring. It is proposed that these factors contribute to the high spring yield in the Swiss populations. Loss of stolon length over each winter was less in the Swiss material, indicating that its good spring growth was not obtained at the expense of winter hardiness. Annual clover yield was found to be significantly positively correlated with the amount of stolon present in spring, exemplifying the importance of stolon survival over the winter.     

STUDIES ON THE ROOT DEVELOPMENT OF HERBAGE PLANTS

Herbage, stubble and root weights were recorded for thirteen months on various leys subjected to two treatments differing in frequency of cutting.
The root-weight per unit area of a ryegrass/white-clover ley was not affected by the frequency of cutting, but the less frequently cut plots had fewer tillers per unit area; the root- and stubble-weight per tiller was, therefore, higher on the less frequently cut plots, On a cocksfoot ley the root-weight was not affected by the cutting treatment, but in late summer and early autumn the stubble-weight was considerably higher on the less frequently cut plots.
When cocksfoot was grown in rows 2 feet apart, the more frequent cutting decreased both the root- and stubble-weight.
On grass leys root-weights were heaviest in the summer and thereafter decreased until the following spring.
The root-weight of lucerne decreased from May to July, increased to a maximum in December and then decreased in weight. The changes in root-weight under lucerne leys were mainly due to changes in the weight of the tap-roots.
The vertical distribution of roots was recorded, and, in the case of the cocksfoot in rows, the lateral distribution of roots is also given.     

The silage yield potential of hybrid ryegrass over a 5-year period in Northern Ireland relative to other ryegrasses and timothy

The yield and persistency of grass species within a managed sward are a major consideration when determining species mixtures for either long-term or short-term sward management systems. Perennial ryegrass is lower yielding but more persistent than Italian ryegrass. Hybrid ryegrass, an interspecies cross between perennial and Italian ryegrass, may be higher yielding than perennial ryegrass and more persistent than Italian ryegrass. Therefore, the yield and persistency of hybrid ryegrass was investigated in Northern Ireland in five experiments, each harvested over five consecutive years, and compared with that of Italian ryegrass, perennial ryegrass and timothy. The performance of the five grass species were in the expected order; Italian ryegrass was higher yielding than hybrid ryegrass, which was higher than perennial ryegrass, with timothy the lowest, whereas perennial ryegrass and timothy had significantly higher sward densities than the hybrid and Italian ryegrasses. In addition, it was found that the rate of decline in yield and sward density with age was the same for all species. Consequently, this study indicated that the higher yielding Italian and hybrid ryegrasses could continue to out-perform perennial ryegrass for 5 years, opening the possibility of more extensive farming use of these species, particularly in Northern Ireland.     

SEASONAL VARIATION IN APPEARANCE AND GROWTH OF GRASS ROOTS

Data from two field experiments, and from a root observation trench, suggest an annual cycle of root growth by grass swards. The production of adventitious roots from the tillers of four grass species, and from perennial ryegrass in a grass/clover sward varied greatly during the year. They were produced in increasing numbers through late winter to early spring; this rate fell sharply in April or May, and was low in summer. The differences in this annual cycle between grass species were small. Examination of roots of perennial ryegrass in a glass-sided trench showed that root elongation was most rapid in summer and was largely confined to the lower soil horizons. Rather more roots were produced in early autumn than during summer, but the rate of elongation was slow from October until spring.
Individual roots of grasses appeared to survive for limited periods. Their longevity depended upon the time of year in which they were first formed; those produced in autumn or winter lived longer than those produced in spring or summer.     

THE EFFECT OF SHOOLY LARVAE (DIPTERA) ON GRASS YIELD

Elimination of shooly larvae increased grass yields daring the September feeding period. Increases were usually less than 10% bat in one case, where growth was abnormally slow, die increase was over 30% and in another, where the larval population was atypically dense, it was almost 60%. The effects of damage were not cumulative daring the feeding period; and at the end of this period insecticidreated plots sometimes gave lower yields than untreated plots. Fertilizer increases had a much greater effect on yield than did control of larvae and, where N was applied at 75 kg/ha (67 lb/ac) in spring, the additional effect of larval control was usually negligible. It is suggested that shoot-fly damage may be more serious in other localities. When turf containing shoot-fly larvae was ploughed, Oscinella spp. re-established themselves more successfully than others in seedling ryegrass bat did not damage the new sward appreciably.     

THE EFFECT OF DEFOLIATION ON THE SEED-PRODUCING CAPACITY OF BRED VARIETIES OF GRASSES

Leafy grass varieties S24 perennial ryegrass, S26 cocksfoot, S215 meadow fescue and S51 timothy were cut in October and at various growth stages in spring; S53 meadow fescue was grazed at similar periods. Sub-plots were given different applications of N. The control plots had similar manurial treatments but were not defoliated. The seed yield of S24 following October cutting was significantly lower than the control in the first harvest year and significantly higher in the second; the mean yields over 2 years, as a result of cutting at this stage and before ear formation in March, were very similar to those of the control treatment. The mean yields from 3 harvest years for S26, S215 and S51 were generally improved by cutting in October and before ear formation, but cutting after ear formation reduced the number of heads and the seed yields. Extra N applied in conjunction with the defoliation had no significant effect on the number of seed heads or on seed yield. Double grazing of S53 in Sept. and Dec. reduced significantly its seed production in both harvest years. Application of N in spring gave a greater response than a similar application in autumn.     

Relationships between the yield of perennial ryegrass and of small-leaved white clover under cutting or continuous grazing by sheep

Seven varieties or advanced breeding lines of white clover ( Trifolium repens L.), all of small leaf size, were grown separately in mixtures with perennial ryegrass ( Lolium perenne L.) in an experiment encompassing three harvest years. Harvestable dry-matter (DM) yield measurements were taken of these mixtures and of perennial ryegrass monocultures under two management regimes: cutting and continuous sheep grazing. Considerable differences were observed in the harvestable DM yields of white clover, perennial ryegrass and total yields of the mixtures between plots containing different white clover varieties. White clover yields were generally higher under cutting, and perennial ryegrass yields were higher under grazing. The difference between perennial ryegrass yield in monoculture and in mixture was variable. In the second harvest year, a significant interaction effect was seen between management and white clover variety for white clover yield but not for perennial ryegrass yield. The relationship between clover yield and grass yield differed between the two management regimes. Under cutting, a negative correlation was observed, indicative of competitive effects. However, under grazing, no such correlation was seen. Possible mechanisms underlying these outcomes are discussed.     

Tillering and leaf area index in grasses in the vegetative phase

The relationship between tillering of a vegetative grass stand and Leaf Area Index (LAI) has been studied during sward establishment of perennial ryegrass and Italian ryegrass with different seedling densities and nitrogen fertilizer rates and on established swards of tall fescue during winter and early spring after different dates of last cut in autumn.
The different experiments on different species led to the conclusion that in every instance the tillering rate slows down as soon as the LAI reaches a value of 3 and terminates rapidly at higher LAI, which corresponds with the almost complete extinction of light at the level of the tiller buds. The results confirm the direct role of light on morphogenesis previously demonstrated by several authors. The existence of such a limit of LAI helps to explain some contradictory effects of temperature or nitrogen on tillering.     

Breeding perennial grasses for forage usage: An experimental assessment of trait changes in diploid perennial ryegrass (Lolium perenne L.) cultivars released in the last four decades

Breeding to improve perennial grasses for forage usage aims to increase the whole-plant aerial biomass. However, the ability to increase the whole-plant yield by breeding may be questionable in grasses which are expected to reach an optimal leaf area index during grass stand growth rather rapidly. We carried out a field experiment aiming to compare herbage yield and other performances of seven natural populations and 21 cultivars of diploid perennial ryegrass registered on European National lists in the last 40 years. Cultivars were sampled in the spike emergence earliness range used for pasture usage, i.e. in the semi-late and late earliness range according to the French classification into spike emergence earliness groups. Forage performances of populations and cultivars were tested in four locations in monthly cut dense sward plots. Morphological and phenological traits were recorded in two spaced-plant experiments, and seed yield was assessed in two locations in standard seed production conditions. Trait regression on cultivar registration year pointed out that total dry-matter yield of cultivars increased by +3.2% per decade. However, dry-matter yield was not improved in the most favourable period for grass growth, i.e. in spring. On the other hand, it was improved in summer (+2.8% per decade) and autumn (+7.4% per decade) when the aerial biomass production is on average rather small. Breeders succeeded in significantly reducing aftermath heading. Reduction in aftermath heading is likely to have contributed to the noted increase in summer and autumn dry-matter yields, and to a moderate improvement in herbage feeding value. Breeding was also efficient in improving rust resistance and in bringing forward the start of spring growth. For monthly cut swards, we evidenced a clear association of leaf and lamina lengths with spring and summer dry-matter yields. Breeding for long leaves, or for high leaf elongation rate, should contribute to improve the cumulated intercepted radiation during re-growths, and consequently could be a way of improving the spring dry-matter yield. Seed yield did not improve with breeding for forage performances. However, we did not notice any negative association between the seed yield and traits related with forage usage. Direct selection pressures on seed yield criteria applied at the core of the breeding process should enable to improve the seed yield without any negative impact on forage performances.     

Soil moisture and growth of contrasting varieties of Lolium, Dactylis and Festuca species

Varieties of Italian, perennial and Italian × perennial ryegrasses, tall fescue and cocksfoot were used to determine the effects of soil moisture on grass growth. Weather conditions were monitored and herbage accumulation, leaf extension rate, leaf appearance rate and tillering were recorded under natural (control), covered and irrigated treatments.
Water deficit reduced crop growth rate in the spring and drought was the major factor influencing crop growth rates in all varieties during the summer. The ryegrasses appeared most sensitive to drought, and particularly poor recovery growth was exhibited by the Italian ryegrass RvP and the hybrid ryegrass Snowdon.
Leaf extension rate and leaf appearance rate were both reduced by increasing soil water deficit. Herbage accumulation was increased by irrigation when potential soil water deficits were greater than 100 mm. When water deficits were large, irrigation increased leaf extension more than leaf appearance or tiller number. Increasing moisture deficit had a greater effect upon tiller number than on leaf extension.     

Influence of the fungal endophyte Acremonium lolii on agronomic traits of perennial ryegrass in France

Four sets of half-sib families of perennial ryegrass with (E+) and without (E-) infection with the fungal endophyte Acremonium lolii were established in spaced-plant nurseries and trial plots in 1989 and 1990 at eight locations in France. In the nurseries, families were scored visually for six agronomic traits; in trial plots, dry matter yields or visual scores were obtained for eleven traits in the planting year and in the following two years. Significant differences between E-and E+ families in each set were more frequent in trial plots than in nurseries, and in all cases there was a positive response to A. lolii infection. In the nurseries, the traits showing the greatest number of significant responses to infection were spring growth (3/28 comparisons) and autumn vigour (3/26 comparisons). In trial plots, E+ families had a significantly ( P < 0·05) higher yield than E- families at three dry locations (5/7 comparisons at Clermont-Ferrand, 3/9 at Montours, and 4/7 at Vemeuil I'Etang) in the 1989 sowings. However, in the 1990 sowings, when different families were studied, endophyte infection had no effect on yield at the driest location. Therefore it is concluded that infection of perennial ryegrass with some strains of A. lolii seems to benefit the host in stressful conditions. However, in Europe generally, climatic conditions are not often stressful for perennial ryegrass and the chances of E+ plants being favoured by either natural or artificial selection seem to be low.     

Effects of white clover cultivar and companion grass on winter survival of seedlings in autumn-sown swards

The aim was to study the effects of white clover cultivar and combinations with perennial ryegrass cultivars on seedling establishment in autumn‐sown swards and on winter survival of seedlings. Large‐leaved white clover cv. Alice and small‐leaved white clover cv. Gwenda, and an erect and a prostrate perennial ryegrass cultivar were sown in autumn in pure stands and as four binary grass‐clover mixtures. Mixtures of white clover cv. Huia and Aberherald with perennial ryegrass were also sown. Companion grasses had no significant impact on the establishment of white clover. The number of seedlings of white clover cv. Alice in mixtures (335 m^?2) was higher than cv. Gwenda (183 m^?2) and pure swards had similar white clover population densities as mixed swards. White clover cv. Huia tended to have more seedlings than Aberherald (355 and 205 m^?2 respectively). No stolons were produced prior to a severe winter, because of the late sowing date. Winter survival of clover seedlings was 0·56 in mixtures and 0·69 in pure stands, irrespective of white clover or companion grass cultivar. Stolon development of white clover in autumn is often considered essential for overwintering survival and spring growth. In this study, there was considerable survival of the non‐stoloniferous tap‐rooted seedlings of all four clover cultivars despite a severe winter.     

Some differences between eight grasses within the Lolium-Festuca complex when grown in conditions of severe water shortage

Differences between eight related grasses within the Lolium-Festuca complex in conditions of severe water shortage were investigated. Transparent covers were used to restrict the access of rain to field plots of pure stands of Westerwolds ryegrass ( Lolium multiflorum var. Westerwoldicum), Italian ryegrass ( L. multiflorum ), hybrid ryegrass ( L. multiflorum × L. perenne ), perennial ryegrass ( L. perenne ), meadow fescue ( Festuca pratensis ), Italian ryegrass × meadow fescue, perennial ryegrass × meadow rescue, tall fescue ( Festuca arundinacea ) and three mixtures (Italian ryegrass with perennial ryegrass; Italian ryegrass with tall fescue; perennial ryegrass with tall rescue) grown in west Wales. Westerwolds ryegrass and Italian ryegrass died out after 12–15 months of exclusion of rain; meadow fescue, meadow rescue × ryegrass hybrids and perennial ryegrass died out after ≊ 2 years of exclusion of rain; tall rescue was still alive after 4 years of exclusion of rain. During the 2 years after the year of sowing, the loss of water from the top 1 m of soil was greater with tall rescue than with the other grasses. Tall fescue and perennial ryegrass had a low rate of leaf expansion, a small increase in length of exposed leaf sheath and a high weight per unit area of emerging leaf blade. Tall rescue had a greater number and weight of roots than the other grasses, particularly in the 50–100 cm layer of soil, on an adjoining, uncovered site. The order of suitability of the eight grasses for conditions of severe water shortage appears to be tall fescue > perennial ryegrass > perennial ryegrass × meadow rescue, meadow fescue, Italian ryegrass × meadow rescue, hybrid ryegrass > Italian ryegrass > Westerwolds ryegrass.