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1.
You-Xiu Zheng Ching-Chung Chen Yuh-Kun Chen Fuh-Jyh Jan 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(1):87-95
A putative virus-induced disease showing chlorotic spots on leaves of Phalaenopsis orchids was observed in central Taiwan. A virus culture, phalaenopsis isolate 7-2, was isolated from a diseased Phalaenopsis orchid and established in Chenopodium quinoa and Nicotiana benthamiana. The virus reacted with the monoclonal antibody (POTY) against the potyvirus group. Potyvirus-like long flexuous filament
particles around 12–15 × 750–800 nm were observed in the crude sap and purified virus preparations, and pinwheel inclusion
bodies were observed in the infected cells. The conserved region of the viral RNA was amplified using the degenerate primers
for the potyviruses and sequence analysis of the virus isolate 7-2 showed 56.6–63.1% nucleotide and 44.8–65.1% amino acid
identities with those of Bean yellow mosaic virus (BYMV), Beet mosaic virus (BtMV), Turnip mosaic virus (TuMV) and Bean common mosaic virus (BCMV). The coat protein (CP) gene of isolate 7-2 was amplified, sequenced and found to have 280 amino acids. A homology
search in GenBank indicated that the virus is a potyvirus but no highly homologous sequence was found. The virus was designated
as Phalaenopsis chlorotic spot virus (PhCSV) in early 2006. Subsequently, a potyvirus, named Basella rugose mosaic virus isolated
from malabar spinach was reported in December 2006. It was found to share 96.8% amino acid identity with the CP of PhCSV.
Back-inoculation with the isolated virus was conducted to confirm that PhCSV is the causal agent of chlorotic spot disease
of Phalaenopsis orchids in Taiwan. This is the first report of a potyvirus causing a disease on Phalaenopsis orchids. 相似文献
2.
Tsutomu Matsumoto Hideki Yamamoto Shin-ichi Fuji Masayasu Inoue 《Journal of General Plant Pathology》2007,73(3):222-224
A novel potyvirus, tentatively named Ornithogalum virus 2 (OV-2) because only its nucleotide sequence of the coat protein gene has been revealed, was isolated for the first
time from Ornithogalum thyrsoides. OV-2 had a flexuous particle (700–740 nm in length) and was sap and aphid transmissible. The virus had a narrow host range;
of 36 test plants in 12 families, only O. thyrsoides and O. dubium were infected. Because the virus caused characteristic stripe mosaic on O. thyrsoides, we propose Ornithogalum stripe mosaic virus (OrSMV), instead of OV-2 for the proper name of the virus.
The nucleotide sequence data reported is available in the DDBJ/EMBL/GenBank databases under accession number AB271783. 相似文献
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4.
Kaoru Hanada Fumiyoshi Fukumoto Manabu Kusunoki Mitsuro Kameya-Iwaki Yuko Tanaka Toru Iwanami 《Journal of General Plant Pathology》2006,72(6):383-386
An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two
large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity
with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV.
The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656. 相似文献
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Shin-ichi Fuji Nanae Mochizuki Masashi Fujinaga Makoto Ikeda Kouichi Shinoda Seiji Uematsu Hiromitsu Furuya Hideki Naito Fumiyoshi Fukumoto 《Journal of General Plant Pathology》2007,73(3):216-221
Alstroemeria plants were surveyed for viruses in Japan from 2002 to 2004. Seventy-two Alstroemeria plants were collected from Aichi, Nagano, and Hokkaido prefectures and 54.2% were infected with some species of virus. The
predominant virus was Alstroemeria mosaic virus, followed by Tomato spotted wilt virus, Youcai mosaic virus (YoMV), Cucumber mosaic virus (CMV), Alstroemeria virus X and Broad bean wilt virus-2 (BBWV-2). On the basis of nucleotide sequence of the coat protein genes, all four CMV isolates belong to subgroup IA. CMV
isolates induced mosaic and/or necrosis on Alstroemeria. YoMV and BBWV-2 were newly identified by traits such as host range, particle morphology, and nucleotide sequence as viruses
infecting Alstroemeria. A BBWV-2 isolate also induced mosaic symptoms on Alstroemeria seedlings. 相似文献
7.
<Emphasis Type="Italic">Turnip yellow mosaic virus</Emphasis> isolated from Chinese cabbage in Japan
Namiko Kirino Koji Inoue Koji Tanina Yuya Yamazaki Satoshi T. Ohki 《Journal of General Plant Pathology》2008,74(4):331-334
A virus that caused a distinct yellow mosaic was isolated in Okayama, Japan from Chinese cabbage (Brassica rapa L., Pekinensis group). The virus, with spherical particles ca. 28 nm in diameter, was mechanically transmissible only to
cruciferous species. From the host range, characteristic morphology of virus particles, serology and sequence analysis of
coat protein gene, the causal virus was identified as Turnip yellow mosaic virus (TYMV). Seed transmission of TYMV at 0–2.2% in Chinese cabbage was confirmed. This report is the first of TYMV from Chinese
cabbage and in Japan.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases as accessions AB358971 and AB358972. 相似文献
8.
Hiroyuki UGA Yuki O. KOBAYASHI Kyoji HAGIWARA Yohachiro HONDA Toshihiro OMURA Takahide SASAYA 《Journal of General Plant Pathology》2002,68(4):378-381
The causative virus (isolate No. 4) of gentian (Gentiana spp.) mosaic, which had been identified previously as Clover yellow vein virus (C1YVV) on the basis of host range and serological reactions, was re-identified as Bean yellow mosaic virus (BYMV) on the basis of the nucleotide sequences of the gene for the coat protein (CP) and the 3′-noncoding region, as well
as the predicted amino acid sequence of CP.
Received 16 April 2002/ Accepted in revised form 19 June 2002 相似文献
9.
H. LECOQ M. RAVELONANDRO C. WIPF-SCHEIBEL M. MONSION B. RACCAH J. DUNEZ 《EPPO Bulletin》1994,24(3):555-559
Transgenic Nicotiana benthamiana plants expressing the coat protein of an aphid-transmissible strain of plum pox potyvirus (PPV-D) were infected with an aphid non-transmissible strain of another potyvirus, zucchini yellow mosaic potyvirus (ZYMV-NAT). Non-viruliferous Myzus persicae could acquire and transmit ZYMV-NAT from these plants but not from infected N. benthamiana control plants (not transformed, or transformed by the vector alone). Immunosorbent electron microscopy experiments using the decoration technique revealed that ZYMV-NAT virus particles in the infected transgenic plants expressing the PPV coat protein could be coated not only with ZYMV antibodies but also, on segments of the particles, with PPV antibodies. This suggests that aphid transmission of ZYMV-NAT occurred through heterologous encapsidation, and reveals a potential risk of releasing genetically engineered plants expressing viral coat proteins into the environment. 相似文献
10.
On plants at 59 sugarcane plantations in Central and East Java, Indonesia, we found virus-like symptoms such as streak mosaic.
The virus was transmitted mechanically and was sett-borne. The nucleotide sequence of the coat protein gene had the highest
identity with that of Sugarcane streak mosaic virus (SCSMV) isolate Pakistani. We tentatively designate this isolate as SCSMV-Idn (Indonesia). 相似文献
11.
Tri Asmira Damayanti Desmiarti Susilo Siti Nurlaelah Dewi Sartiami Tetsuro Okuno Kazuyuki Mise 《Journal of General Plant Pathology》2008,74(6):438-442
Severe mosaic with leaf malformation and green vein banding was observed on yam bean in West and Central Java, Indonesia.
Virions of the causal virus were flexuous filaments, about 700 nm in length, with a coat protein of 30 kDa. The virus was
transmitted by mechanical inoculation and by aphids in a nonpersistent manner. The nucleotide sequence of the coat protein
gene had the highest identity with that of Bean common mosaic virus (BCMV, genus Potyvirus) isolate VN/BB2-5. Based on demarcation criteria, including the genome sequence and host range, we tentatively designate
this isolate as BCMV-IYbn (Indonesian yam bean).
The nucleotide sequence reported is available in the DDBJ/EMBL/GenBank databases under accession number AB289438. 相似文献
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A. A. Khan R. Sharma B. Afreen Q. A. Naqvi S. Kumar S. K. Snehi S. K. Raj 《Phytoparasitica》2011,39(2):199-203
Natural occurrence of mosaic disease was observed on basil (Ocimum sanctum L.) in Aligarh, U. P., India, during 2008. The disease could be transmitted by sap inoculations from naturally infected O. sanctum to O. sanctum and some test plant species. Cucumber mosaic virus (CMV) was detected by RT-PCR using coat protein gene specific primers of CMV (Acc. AM180922 & AM180923), which resulted in
the expected size ~650 bp amplicon in infected samples. The amplicon was cloned, sequenced and data were deposited in GenBank
Acc. EU600216. The sequence data analysis revealed 97–99% identities at both nucleotide and amino acid levels with the CMV
strains of subgroup II reported worldwide. Based on the high sequence identities and close phylogenetic relationships with
CMV subgroup II strains, the virus under study has been identified as a new isolate of CMV subgroup II and designated as CMV-Basil. 相似文献
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16.
Eduardo Segundo Dietrich E. Lesemann Germán Martín María P. Carmona Leticia Ruiz Isabel M. Cuadrado Leonardo Velasco Dirk Janssen 《European journal of plant pathology / European Foundation for Plant Pathology》2007,117(1):81-87
Amaranthus leaf mottle virus (AmLMV) was classified as a member of the genus Potyvirus on the basis of its particle morphology, serology, and biological properties (Casetta et al., 1986). Based on these properties, an Amaranthus viridis-infecting virus isolated in Spain, causing mottle and leaf blistering as well as reduced growth has been identified as AmLMV.
The 3′ terminal genomic region of this and a reference isolate from Italy has been sequenced and reveals a 95% nucleotide
identity between the two isolates. The sequenced part comprises the coat protein with 281 amino acids and 315 nucleotides
of the 3′ untranslated region (UTR) preceding a polyadenylated tail. Pairwise comparisons and phylogenetic analysis of the
nucleotide and deduced amino acid sequences of the CP and 3′ UTR of the cloned cDNAs with those of other potyviruses shows
that AmLMV is a distinct potyvirus closely related to Potato virus Y. 相似文献
17.
Ramesh R. Chavan Michael N. Pearson Dan Cohen 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(2):247-259
Actinidia chinensis and A. deliciosa plants from China, showing a range of symptoms, including vein clearing, interveinal mottling, mosaics and chlorotic ring
spots, were found to contain ~300 nm rod-shaped virus particles. The virus was mechanically transmitted to several herbaceous
indicators causing systemic infections in Nicotiana benthamiana, N. clevelandii, and N. occidentalis, and local lesions in Chenopodium quinoa. Systemically- infected leaves reacted with a Tobacco mosaic virus polyclonal antibody in indirect ELISA. PCR using generic and specific Tobamovirus primers produced a 1,526 bp sequence spanning the coat protein (CP), movement protein (MP), and partial RNA replicase genes
which showed a maximum nucleotide identity (88%) with Turnip vein clearing virus and Penstemon ringspot virus. However, when the CP sequence alone was considered the highest CP sequence identity (96% nt and 98% aa) was to Ribgrass mosaic virus strain Kons 1105. The morphological, transmission, serological and molecular properties indicate that the virus is a member
of subgroup 3 of the genus Tobamovirus. 相似文献
18.
Tsutomu Matsumoto Hideki Yamamoto Shin-ichi Fuji Masayasu Inoue 《Journal of General Plant Pathology》2008,74(1):76-80
A potyvirus tentatively named Ornithogalum virus 3 (OV-3) was successfully isolated by single-aphid transmissions from O. thyrsoides mix-infected with OV-3, Ornithogalum
mosaic
virus (OrMV) and Ornithogalum stripe mosaic virus (OrSMV). OV-3, a flexuous, rod-shaped particle of ca. 690 nm, was sap and aphid transmissible. The virus
had a narrow host range and caused necrotic mosaic on O. thyrsoides under cold conditions. We therefore propose the name Ornithogalum necrotic mosaic virus (OrNMV) for OV-3. A synergistic increase in symptom severity was apparent on O. thyrsoides mix-infected with OrSMV/OrNMV, but not with either OrMV/OrNMV or OrMV/OrSMV.
The nucleotide sequence data reported is available in the DDBJ/EMBL/GenBank databases under accession number AB282754. 相似文献
19.
A potyvirus (eggplant mottle virus, EMoV) causing mosaic mottling in eggplant ( Solanum melongena ) was characterized on the basis of biological, serological and partial nucleotide sequence properties. EMoV infected Chenopodium amaranticolor and members of the Solanaceae. Polyclonal antiserum against EMoV showed antigenic relationship with henbane mosaic potyvirus (HMV) and potato Y potyvirus (PVY). Virus-specific antibodies directed to the N-terminal region of EMoV cross-reacted only with PVY. Determination and comparison of nucleotide sequence of the coat protein (CP) and the 3'-untranslated region (UTR) of EMoV with other potyviruses showed that the level of homology was highest with PVY isolates. Comparative sequence analyses of the CP amino acid and 3'-UTR sequences with distinct PVY isolates placed EMoV within the PVYO subgroup. 相似文献
20.
Gerald Ravelo Uiko Kagaya Tsuyoshi Inukai Masanao Sato Ichiro Uyeda 《Journal of General Plant Pathology》2007,73(1):59-65
Clover yellow vein virus (ClYVV) elicits lethal tip necrosis in the pea line PI 118501. Pea line PI 118501 develops necrotic lesions and veinal necrosis
on inoculated leaves, followed by systemic necrosis, leading to plant death. To understand the genetic basis of this lethal
tip necrosis, we crossed lines PI 226564 and PI 250438, which develop mosaic symptoms in response to ClYVV inoculation. In
reciprocal crosses of PI 118501 with PI 226564, all F1 plants had mosaic symptoms with slight stem necrosis and early yellowing of upper leaves. Essentially the same symptom was
manifested in PI 118501 × PI 250438 F1 plants. In F2 populations from the cross between PI 118501 and PI 226564, the observed ratios of necrosis, mosaic with slight stem necrosis,
and mosaic fit the expected 1 : 2 : 1 ratio. These results indicate that a single incompletely dominant gene confers the induction
of necrosis in PI 118501. This locus in pea, conferring necrosis induction to ClYVV infection, was designated Cyn1 (Clover yellow vein virus-induced necrosis). A linkage analysis using 100 recombinant inbred lines derived from a cross of PI 118501 and PI 226564
demonstrated that Cyn1 was located 7.5 cM from the SSR marker AD174 on linkage group III. 相似文献