生长激素（PST）脂质体的药物代谢动力学研究

本文研究了生长激素（PST）脂质体在仔猪体内的药物代谢动力学，分析了血浆中生长激素浓度时间的变化曲线，确定其释药模型为单室模型，求出了各项动力学参数，揭示PST脂质体的缓释效果显著，缓释时间长达7d以上。     

PST脂质体代谢动力学的研究

在猪体内m.i.PST脂质体，研究其在体内的药物代谢动力学和对肥育猪生产能力的影响，分析其血浆中生长激素浓度随时间的变化曲线，确定其释药模型为单室模型，求出各项动力学参数，结果为PST脂质体的缓释效果显著，缓释时间长达7d以上。     

生长激素(PST)脂质体的药物代谢动力学研究

本文研究了生长激素(PST)脂质体在仔猪体内的药物代谢动力学,分析了血浆中生长激素浓度随时间的变化曲线,确定其释药模型为单室模型,求出了各项动力学参数,揭示PST脂质体的缓释效果显著,缓释时间长达7 d以上.     

不同组份对猪生长激素脂质体化学稳定性的影响

本文通过逆相蒸发法制备猪生长激素（PST）脂质体，重点是探讨不同组份原料对PST脂质体化学稳定性的影响。经过试验论证，加入抗氧化剂后，包封率明显提高，而且加BHT的效果比加维生素E（VE）的效果要好。通过对大豆卵磷脂和蛋黄卵磷脂的对比实验，证明了蛋黄卵磷脂所制脂质体效果较好，通过测定酸价，丙二醛含量的变化也证明了这一点。为PST脂质体的临床应用做初步的研究。     

生长激素（PST）脂质体对育肥猪生产性能的影响

选择18头体重20kg左右的太湖二元杂交小公猪，分成A、B、C3组，每组6头。A组为对照组；B组颈部肌肉注射生长激素（PST）4ml/头；C组颈部肌肉注射PST脂质体28mg/头；结果表明PST与PST脂质体对育猪的生产性能影响较为接近，与对照组相比，均可显著提高育肥猪的日增重，饲料报酬，改善猪的胴体品质，说明PST脂质体可代替PST用于生产实践。     

不同组份对猪生长激素脂质体化学稳定怀的影响

本文通过逆相蒸发法制备猪生长激素(PST)脂质体,重点是探讨不同组份原料对PST脂质体化学稳定性的影响.经过试验论证,加入抗氧化剂后,包封率明显提高,而且加BHT的效果比加维生素E(VE)的效果要好.通过对大豆卵磷脂和蛋黄卵磷脂的对比实验,证明了蛋黄卵磷脂所制脂质体效果较好,通过测定酸价,丙二醛含量的变化也证明了这一点.为PST脂质体的临床应用做初步的研究.     

生长激素(PST)脂质体对育肥猪生产性能的影响

选择18头体重20 kg左右的太湖二元杂交小公猪,分成A、B、C 3组,每组6头.A组为对照组;B组颈部肌肉注射生长激素(PST) 4 ml/头;C组颈部肌肉注射PST脂质体28 mg/头;结果表明PST与PST脂质体对育肥猪的生产性能影响较为接近,与对照组相比,均可显著提高育肥猪的日增重,饲料报酬,改善猪的胴体品质,说明PST脂质体可代替PST用于生产实践.     

猪生长激素(PST)脂质体代谢动力学的研究

在猪体内注射脂质体 ,研究其在体内的药物动力学 ,分析其血浆中生长激素浓度随时间的变化曲线 ,确定其释药模型为单室模型 ,求出各项动力学参数 ,结果为PST脂质体的缓释效果显著 ,缓释时间长达7天以上     

PST(生长激素)脂质体对生长猪生产性能的影响

PST脂质体具有显著的缓释效果,本文研究其对生产能力的影响。结果发现PST脂质体效果与PST效果相一致,对猪的增重、瘦肉率及料肉比有明显促进作用,瘦肉率提高达5-10％,料肉比降低。     

PST脂质体在猪血浆中稳定性的研究

PST脂质体将应用于养猪业,并且注射于猪血液中,因而在血液中的稳定性很重要.脂质体在体液中大豆稳定性是发挥药物载体的作用的关键.血液中有多种破坏因素 [13];PST脂质体在进入血循环到达靶位之前,应该保持比较完整的形态才能在体内有效地降低副作用,更好地发挥疗效,因此在本研究中,对PST在猪血中的稳定性进行初步探讨.     

Long-term recombinant porcine somatotropin (PST) treatment mitigates the responses to subchronic lipopolysaccharide in swine

The effect of multiple lipopolysaccharide (LPS) challenges in swine undergoing long-term treatment with porcine somatotropin (PST) was determined. Changes in aspartate serine transaminase (AST) occurred only at 24h following the first LPS challenge dose (P<0.05), while PST treatment moderated any change from occurring. Nonesterified free fatty acid (NEFA) levels were elevated in PST treated animals for the first 3 days following daily LPS treatment (P<0.05), while LPS treatment alone had no effect on plasma NEFA levels. Plasma urea nitrogen (PUN) levels were unchanged by LPS following the initial LPS challenge, but were decreased following the second challenge dose (P=0.014). These changes were long lasting, with a return to normal PUN levels not evident until Day 6. The PST treatment mitigated changes in PUN (P<0.05) when LPS was administered. Haptoglobin plasma levels, along with lipid peroxide production were not affected by LPS challenge or PST administration. LPS challenge reduced the levels of immunoreactive heat shock protein 70 (HSP70) throughout the entire challenge period (P<0.001). PST-LPS animals had normal levels of this protein. The results of the present study demonstrate that long-term PST treatment mitigates the adverse effects of subchronic LPS administration.     

Stability of vitamin E in blood and plasma from cattle, sheep, and pigs.

The stability of alpha-tocopherol concentrations in sheep, cattle, and pig blood and plasma stored at different temperatures was examined. For all species, the vitamin was stable for at least 6 days in plasma stored at -20 C, 4 C, and 25 C and in blood stored at 4 C and 25 C. For sheep and cattle, the vitamin was stable for at least 6 days in plasma stored at 37 C, but it was unstable in blood from all species stored at 37 C and in pig plasma stored at 37 C.     

连翘酯苷脂质体在雏鸡体内的药动学研究

The study was aimed to evaluate the pharmacokinetics and tissue distribution of forsythiaside liposome in chicks by intravenous administration. The pharmacokinetics of chicks with intravenous administration of forsythiaside liposome and forsythiaside solution 20 mg/kg was studied and the concentrations of forsythiaside in heart, liver, spleen, lung, kidney and plasma were determined by HPLC. The plasma concentration-time curves of forsythiaside liposome and forsythiaside solution were both fit to the two-compartment model and the pharmacokinetic parameters were (1.79±0.050) and （0.11±0.006） h for t_1/2β, (39.95±2.32） and (4.26±0.39) (μg·h)/mL for AUC, (0.56±0.04） and （4.73±0.41） mg/(h·kg) for CLs. Compared with the liposome to the solution, the drug distribution in liver, spleen and lung were obviously elevated. Compared with forsythiaside solution, the forsythiaside liposome could significantly prolong the resident time of forsythiaside in the blood circulating system and could be concentrated at the target tissue rich in the reticuloendothelial system.     

The influence of thermal load on the immunochemical detection of dried blood plasma supplements in meat mixtures

Addition of blood plasma to meat products is not permitted in the FRG unless these products are heat processed using an internal temperature of 80 degrees C (German regulation of meat and meat products: "Verordnung für Fleisch und Fleischerzeugnisse"). Such heat process may have an unfavourable effect on the detectability of blood plasma. Since blood plasma or dried plasma may originate from different animal species (porcine or bovine) two different anti dried blood plasma-sera (porcine and bovine) are required for immunochemical analysis. The varying quality of these sera has to be considered when interpreting the results. Seven M urea extract turned out to be suitable for detection of dried plasma additives and proved to be highly effective particularly when examining heated samples. Both the gel-diffusion and the electro immuno assay proved useful for the detection of dried blood plasma, provided the examined extracts had been adequately diluted. Immunochemical reactivity was hampered by the heat process which was given to the sample. Accordingly, the concentration of the plasma in a particular sample cannot be determined unless the time/temperature data of the process applied to the sample were given and model samples were tested for comparison.     

Localization of hyaluronic acid in the seminal vesicles of the miniature pig

We studied the detailed localization of hyaluronic acid in the seminal vesicles of the miniature pig, using hyaluronic acid-binding protein as a specific histochemical probe at the ultrastructural level. According to the results, the basolateral surface of the plasma membrane of the glandular epithelial cells, was found to contain hyaluronan. However, abundantly present was hyaluronan in the subepithelial connective tissue, in particular, in the extracellular matrix surrounding the fibroblasts, smooth muscle cells, small blood vessels and capillaries. The substance was also observed in the surface coat of the plasma membrane of the fibroblasts, but not in that of the smooth muscle cells. The findings suggest that hyaluronan in the seminal vesicles of the miniature pig is synthesized onto the surface coat of the plasma membrane of the fibroblasts, is contributed to the extracellular matrix, and consequently concentrates in the subepithelial connective tissue. The substance may particularly be involved in a variety of cellular functions to maintain morphological organization as well as to regulate physiological homeostasis in the reproductive organ of this species, rather than participate in sperm functions.     

四种猪血抗凝剂抗溶血效果比较及新复合型抗凝剂最佳使用条件研究

采用紫外分光光度计测定血浆液中血红蛋白的吸光值。比较添加不同抗凝剂血液保存时间,确定一种猪血新复合型抗凝剂及其最佳保存条件。试验结果表明,最佳使用条件为抗凝剂溶液浓度15%、抗凝剂添加比例4.5‰、保存温度8℃、保存时间不超过84h。     

日粮纤维调控猪体粪臭素水平的研究进展

粪臭素(3-甲基吲哚)是猪大肠内微生物降解L-色氨酸的产物,是引起公猪膻味的主要物质,易在脂肪组织中沉积,对猪肉风味有负面作用。本文综述了粪臭素的生物合成和代谢途径,以及日粮纤维对猪肠道、血浆、粪便和脂肪组织中粪臭素水平影响的研究进展;并从粪臭素的产生、吸收和代谢等方面,详细讨论了纤维物质影响猪体粪臭素沉积的可能机制。