Assessment of hygienic quality of haylage fed to healthy horses

Wrapped forages such as haylage and silage are increasing in popularity in equine feed rations, but knowledge of their microbial composition, especially of haylage, is scarce. Haylage may be more at risk of mould growth compared to silage, and methods for the assessment of hygienic quality of haylage are needed. Varying culturing conditions, including aerobic/anaerobic incubation, four substrates and three incubation temperatures were therefore evaluated for analysis of fungi in haylage samples from eighteen horse farms, taken on two occasions (autumn and the following spring). Average mould counts in forage samples were low, but fungi with the potential for producing mycotoxins, such as Aspergillus fumigatus and Penicillium spp., were present. The microbial composition and content of fermentation products were similar in haylage samples taken in autumn and in spring. The type of substrate used for cultivation did not influence mould or yeast counts, but not all mould species were present on all substrates. Incubation temperature influenced fungal counts and the presence of mould species. By using at least two substrates (malt extract agar and dichloran 18% glycerol agar) and two incubation temperatures (25 and 37°C), all mould species/genera that were identified in this study could be detected.     

Effect of anaerobic digestion residue vs. livestock manure and inorganic fertilizer on the hygienic quality of silage and haylage in bales

An increased recycling of nutrients from organic waste to support feed and food production is important for achieving sustainability. However, organic waste may contain undesired microorganisms that may increase the risk of impaired hygienic quality of feeds when used in forage‐cropping systems. This study aimed to investigate how the hygienic quality of silage and haylage was affected after fertilization of grassland with organic fertilizers [anaerobic digestion residue (ADR) pasteurized before spreading, or liquid cattle manure] compared with inorganic NPK fertilization. The experiment was performed on the same grass ley for 2 years, with fertilization carried out before each harvest (year 1, two harvests; year 2, one harvest). The crop was conserved as silage (300 g DM kg^?1) and haylage (500 g DM kg^?1) in bales (ca 40–55 kg) stored for 1, 5 or 10 months before opening, including effects of storage time and conservation method (silage or haylage) on hygienic quality. Results showed that ADR‐treated crops did not produce silage or haylage with higher microbial counts in comparison with manure‐treated crops on any occasion. The fermentation pattern in silage and haylage from ADR‐treated crops was similar to the pattern in manure‐treated crops. The use of inorganic fertilizer sometimes produced lower pH and higher content of lactic acid in silage, compared with using organic fertilizers. In conclusion, ADR could be used as an organic fertilizer for forage crops without increased risk of impaired hygienic quality compared with using liquid manure or inorganic NPK fertilizers.     

Cutting frequency vs. N application: effect of a 20‐year management in Lolio‐Cynosuretum grassland

The aim of this study was to determine the effects of different long‐term management options on re‐establishment of traditional species‐rich grassland in a Lolio‐Cynosuretum grassland. The experiment was set up in 1987 in a low‐fertilized mown pasture in the Eifel Mountains (Germany). Two and four cuts per year each with three levels of nitrogen fertilizer application were compared to no application of P and K fertilizer. Biomass production increased as a result of N application, whereas crude protein content and digestibility of organic matter improved in the four‐cut treatments. During a 20‐year period, we found a significant shift in dominant grass species without any significant effect on species richness. Lolium perenne and Elytrigia repens were replaced by Holcus lanatus and Alopecurus pratensis, especially in the two‐cut treatments with N application. In the unfertilized plots, several individuals of Platanthera bifolia appeared when swards were cut only twice per year, probably as a result of nutrient depletion together with low cutting frequency. An increase in number of cuts mainly supported plant species well adapted to intensive management, even under low N application and relatively low plant‐available P content in the soil. Therefore, we conclude that cutting frequency was a key driver on plant species composition in this experiment. The re‐establishment of traditional species‐rich grassland from intensively managed Lolio‐Cynosuretum was not achieved within 20 years of cutting management combined with absence of P and K application.     

Hay production from lowland semi-natural grasslands: a review of implications for ruminant livestock systems

Data on total, seasonal and between-year variability in the productivity and nutritional quality of cut forages obtained from a range of lowland semi-natural grasslands are reviewed. Dry-matter production, as hay, from unfertilized species-rich semi-natural grasslands ranged from less than 20% to about 80% of the production that might be expected from agriculturally improved and intensively managed grasslands. Unfertilized species-rich grasslands had lower growth rates, reflecting low nutrient availability in spring and early summer. Low soil phosphorus availability appears to be a key factor allowing high species-richness to be maintained. The in vitro digestibilities of herbage cut for hay from semi-natural grasslands were 20%, or more, below values for conserved forages from intensively managed grassland. When losses incurred during the hay-making process were taken into account, then the nitrogen content of hays from many semi-natural grasslands appear to be sub-optimal for productive livestock. Metabolizable energy values of herbage cut for hay were 10–40% below values for intensively managed conserved grass. Phosphorus content of forages from most species-rich grasslands was either below the metabolic requirement of livestock or inadequate to sustain high individual animal performance. Potential problems associated with supplementation of the diet with phosphorus, and with high calcium-to-phosphorus ratios, are discussed. Although potassium content of most of the herbage cut for hay was adequate for productive ruminant livestock, exceptions arise with hay cut after July. The magnesium content of cut herbage from some semi-natural grasslands was below an adequate level for productive livestock. Most cut herbage appeared to contain adequate amounts of sodium. Inadequacy of the mineral content of hays from semi-natural grasslands for ruminant livestock will be exacerbated by nutrient losses incurred during the hay-making process and particularly so when hay- making is prolonged by wet weather. Few feeding studies using hays from semi-natural grasslands of high nature conservation interest have been conducted. Lack of such data makes it difficult to plan any integrated use of these hays in ruminant feed budgets for livestock systems.     

Factors affecting digestibility of temperate forages from seminatural grasslands: a review

Abstract To indicate possibilities for the use of forages from seminatural grasslands in ruminant production systems, a literature study to describe the nutritive value of those forages was carried out. In species‐rich grasslands, the digestibility of forages is usually lower than the digestibility of forages produced by grasslands used for intensive production. There is also more variation within a species‐rich sward, because of different stages of maturity of the individual species and because of likely variations in digestibility among forage species independent of stage of maturity. Moreover, the presence of dicotyledonous species may have a positive or negative effect on digestibility. In forages from seminatural grasslands, the relationship between chemical composition and digestibility differs from that of Lolium perenne, which is often used as a research standard. Therefore, predictions of the digestibility of species‐rich forage can be inaccurate. The relationship between in vivo and in vitro digestibility may also be different from the relationship that is common for L. perenne. In some cases, the in vivo digestibility is higher than the in vitro value, as calibrations are based on L. perenne. Therefore, the quality of forages from seminatural grasslands might be higher than predicted, and this may increase the scope for practical use of this kind of forage in ruminant nutrition systems.     

Chemical composition and in vitro total gas and methane production of forage species from the Mid Rift Valley grasslands of Ethiopia

There is increasing interest in sustainable land use in the tropics to optimize animal production while also reducing methane (CH₄) emissions, but information on nutritive value and CH₄‐emission potential of tropical forage species is limited. Samples of 24 grasses and five other forages were collected during the main rainy season on randomly positioned quadrats in semi‐arid grassland in the Mid Rift Valley of Ethiopia. Samples were pooled by species, analysed for chemical composition and incubated with rumen fluid to determine total gas and CH₄‐emission potentials using a fully automated in vitro gas production apparatus. Organic matter digestibility (OMD) and metabolizable energy (ME) contents were calculated from chemical composition and gas production data. Large variability was observed among forages for all nutritional variables considered. The grasses Eleusine multiflora, Pennisetum stramineum, Dactyloctenium aegyptium, Eragrostis aspera, Cenchrus ciliaris and Eragrostis cilianensis showed relatively high OMD (68–72%) and ME values (9·1–10·2 MJ kg^?1 dry matter). Melinis repens, E. multiflora and the non‐legume forb Zaleya pentandra showed relatively low CH₄ to total gas ratios; these species may have potential for use in low CH₄‐emission forage diets. Acacia tortilis fruits had high content of crude protein and moderate ME values, and may be an ideal feed supplement for the grazing ruminant. Sodium content was below the recommended level for ruminants in all the forage species. Overall, the pasture stand during the main growing season was evaluated as having moderate nutritional quality.     

Annual forage legumes in dryland agricultural systems of the West Asia and North Africa Regions: research achievements and future perspective

Forage legumes are vitally important to animal production in the dryland farming systems of the Mediterranean region. Of the diverse forage‐legume species adapted to the Mediterranean climate, vetches, (Vicia spp.), chicklings (Lathyrus spp.), annual medics (Medicago spp), clovers (Trifolium spp.) and species of the Lupinus, Lotus, Onobrychis, Hedysarum and Ornithopus genera are considered to be the most agronomically important and economically valuable species for the region. Adoption of perennial self‐regenerating medic (Medicago spp.) has been limited because of technical difficulties, but annual vetch (Vicia spp.) has the greatest potential as a viable animal‐feed source and a rotation crop with cereals. Some forage legumes survive harsh conditions by their unique underground growth habit, for example, V. amphicarpa and Lathyrus ciliolatus. Efforts to improve forage legumes have been based on both management/cultural factors and breeding. Research based on several long‐term barley‐ and wheat‐based rotation trials has demonstrated the viability of forage legumes, especially vetch, in the region's improved farming system. An additional benefit to such legumes is the enhancement of soil quality, that is, soil fertility, soil organic matter and soil physical properties. Thus, the development of forage legumes is essential to agricultural sustainability in the Mediterranean region and in other dryland cereal‐growing areas of the world where grazing livestock is a dominant enterprise. To build upon the considerable research conducted on forages, intensified efforts are needed to develop locally adapted forage cultivars, to provide economic assessment of forages in cropping systems and to promote technology transfer at the farm and community level.     

In vitro fermentation patterns and methane production of sainfoin (Onobrychis viciifolia Scop.) hay with different condensed tannin contents

Sainfoin (Onobrychis viciifolia Scop.) is a perennial legume recently reappraised for some positive characteristics leading to highly satisfactory animal performance. Sainfoin’s characteristics may be partly explained by the presence of moderate levels of condensed tannins (CTs) able to protect dietary protein from microbial degradation in the rumen. Decreased CH₄ emissions have been reported for ruminants consuming CT‐containing forage. The aim of this study was to evaluate the effects of CT content on the in vitro fermentation characteristics and kinetics and methane production of four samples of O. viciifolia cut at different phenological stages. Sainfoin hays and one sample of alfalfa hay were incubated at 39°C in anaerobiosis using the in vitro gas production technique. The chemical composition, tannin content and fermentation characteristics and kinetics of sainfoin samples were significantly affected by phenological stage. After 48 h, the CH₄ production in sainfoin hays showed a tendency to increase with the advancement of phenological stage [from 38·6 to 49·8 mL g⁻¹ of degraded organic matter (OM)]. The best period to cut sainfoin for hay making is between early and late flowering, when the forage combines high OM digestibility, low CH₄ production and more efficient microbial fermentation.     

Effect of six-weekly harvests on the yield, chemical composition and dry matter degradability of Panicum maximum and Stylosanthes hamata in Nigeria

The dry matter (DM) yield and degradability of 6‐week‐old harvests of tropical forages were measured over a season. The forages were nitrogen‐fertilized Guinea grass (Panicum maximum, NFG), unfertilized Guinea grass (UFG), Verano stylo (Stylosanthes hamata,VS), a Guinea grass–Verano stylo mixture (GSM) and Guinea grass in the grass–Verano stylo mixture (GGSM). Six‐week‐old forages were made possible through a cutting regime, which produced four harvests in the growing season. The DM yields of the forages differed significantly (P < 0·001) and showed a significant reduction (P < 0·01) across the season. Crude protein and neutral‐detergent fibre concentrations were significantly (P < 0·01) different between the forages but there was no difference between harvests. The DM degradability of the forages at all harvests were significantly (P < 0·001) different with differences in the soluble fraction (a), degradable fraction (b), potential degradability (PD) and effective degradability (ED), but rate of degradability (c) did not show any significant difference between the forages. Significant (P < 0·01) differences were found between harvests for b and PD, and for the interaction between forage and harvest for b, PD and ED but were not found for the a and c fractions. Both the PD and ED values of all the forages fell with advancing harvests. Although the 6‐week‐old harvests of forage were found not to influence the characteristic reduction in yield of tropical grasses over time, it is concluded that such a management system could be used to obtain forage of relatively high nutritive value during the growing season.     

Shoot characteristics of Trifolium repens grown in association with Lolium perenne or Holcus lanatus in pastures grazed by sheep

The morphological characteristics of Trifolium repens, grown in association with either Lolium perenne or Holcus lanatus and grazed by sheep to maintain sward surface heights of 3 cm, 5 cm and 10 cm, were measured. The lamina area, petiole length, internode length and internode weight of T. repens were significantly greater when H. lanatus was the companion grass than when L. perenne was the companion grass. All of these characteristics, together with the number of laminae per stolon, were also greater in the taller swards than in the shorter swards. The number of branches on each shoot did not vary significantly with either companion species or sward height treatment. Except for the effects of the treatments on the number of branches, the results are generally consistent with the known effects of variation in foliar shading on the morphology of T. repens.     

Changes in species composition of abandoned sown swards after imposing seasonal cutting treatments

Abstract Seasonal cutting treatments were imposed on abandoned perennial ryegrass/white clover swards at three sites in the UK to determine the impact on species diversity and the contribution of different species to biomass. Before the cutting experiment, the abandoned swards had received no fertilizer applications and had been neither grazed nor cut for 3 years. The original sown species, Lolium perenne and Trifolium repens, had virtually disappeared during this period. Self‐sown grasses, including Agrostis capillaris, Dactylis glomerata, Festuca rubra, Holcus mollis, Poa pratensis and P. trivialis, had become dominant at two sites. Ranunculus repens was the dominant species at the third site, and self‐sown grasses, such as Holcus lanatus and P. trivialis, and Juncus and Carex species were also present. The standing herbage in 2·5 m × 2·5 m subplots was cut to 2·5 cm above ground level and removed annually in either June or October for 7 years at two sites and 6 years at the third site. Total biomass removed was generally < 4 t dry matter (DM) ha^?1, and both total and live biomass decreased over time in the June cutting treatment at two sites. There were large changes in species composition after initiating the two cutting treatments. The numbers of grass and dicot species increased, although many new species had a low frequency of occurrence. The contribution of species to biomass changed over time, with species showing a vegetative regenerative strategy decreasing over time at one site. At this site, annual cutting in June was slightly more effective than cutting in October in reducing the dominance of R. repens; cutting in early October effectively reduced Juncus spp. At two sites, there was a decrease in the contribution of the species group showing seasonal regeneration by seed, and differences between cutting dates for two species within this group at one site. L. perenne and T. repens remained at much lower levels than in adjacent unfertilized, grazed swards. Reseeding may be necessary to create species assemblages for ecological and agricultural objectives.     

Effects of sheep stocking on the plant community and agricultural characteristics of upland Anthoxanthum odoratum–Geranium sylvaticum meadow in northern England

In the UK, upland hay meadows (Anthoxanthum odoratum–Geranium sylvaticum grassland) with high plant biodiversity are rare and confined to submontane areas of northern England. We report results from a 5‐year experiment to test suggestions that recent biodiversity declines were attributable to increased sheep‐stocking density and a longer spring stocking period, thus delaying the shut up date for the growth of the hay crop. Longer stocking periods and higher stocking densities decreased the forage mass at 8 July, but they increased herbage N content and digestibility, reduced plant species diversity and reduced populations and seed production of Rhinanthus minor. Compared with unstocked swards, the similarity of the vegetation to Anthoxanthum odoratum–Geranium sylvaticum grassland was reduced by 16·9% when stocking with sheep continued until 27 May each year, and by 8·3% when sward heights were maintained at 3 cm compared with 5 cm. Increased mean sward height and height of R. minor were positively correlated with accumulated temperatures. Results support suggestions that recent reductions in the nature value of these grasslands might be a consequence of high stocking densities persisting until later in the spring, carried out during a 1‐year period with warmer temperatures.     

Forage herbs improve mineral composition of grassland herbage

Provision of an adequate mineral supply in the diets of ruminants fed mainly on grassland herbage can present a challenge if mineral concentrations are suboptimal for animal nutrition. Forage herbs may be included in grassland seed mixtures to improve herbage mineral content, although there is limited information about mineral concentrations in forage herbs. To determine whether herbs have greater macro‐ and micromineral concentrations than forage legumes and grasses, we conducted a 2‐year experiment on a loamy‐sand site in Denmark sown with a multi‐species mixture comprised of three functional groups (grasses, legumes and herbs). Herb species included chicory (Cichorium intybus L.), plantain (Plantago lanceolata L.), caraway (Carum carvi L.) and salad burnet (Sanguisorba minor L.). We also investigated the effect of slurry application on the macro‐ and micromineral concentration of grasses, legumes and herbs. In general, herbs had greater concentrations of the macrominerals P, Mg, K and S and the microminerals Zn and B than grasses and legumes. Slurry application indirectly decreased Ca, S, Cu and B concentrations of total herbage because of an increase in the proportion of mineral‐poor grasses. Our study indicates that including herbs in forage mixtures is an effective way of increasing mineral concentrations in herbage.     

Adaptability and productivity of some warm-season pasture species in a Mediterranean environment

Warm‐season grasses and legumes have the potential to provide forage throughout the Mediterranean summer when there are high temperatures and low rainfall and when cool‐season grasses become less productive. Twenty‐nine non‐native, warm‐season pasture species (twenty‐three grasses and six legumes) were assessed for their adaptability to the coastal plain of southern Italy in terms of their productivity and nutritional quality. The investigated species were compared with two reference species widely used in a Mediterranean environment: a grass (Festuca arundinacea) and a legume (Medicago sativa). The species differed in their phenological and biological characteristics, i.e. start of vegetative resumption, first flowering and cold resistance, from each other and from the control species. From the second year after establishment, warm‐season perennial grasses had high dry‐matter (DM) yields and, in many cases, a more than adequate nutritional quality. As for legumes, the control, M. sativa gave the best results in all the investigated characters. Among the grasses, seven species (Chloris gayana, Eragrostis curvula, Panicum coloratum, Paspalum dilatatum, Pennisetum clandestinum, Sorghum almum, Sorghum spp. hybrid) had DM yields greater than the control species and had their maximum growth during the hottest period of the year, when F. arundinacea, the control grass species, was dormant. Eragrostis curvula had the highest annual DM yield (21·1 t ha^?1) and P. clandestinum provided the best combination of agronomic and yield characteristics which were similar to those of M. sativa. The seven above‐mentioned species have the potential to supply hay or grazing and contribute to broadening and stabilizing the forage production calendar in Mediterranean‐type environments.     

Yield response of long-term mixed grassland swards and nutrient cycling under different nutrient sources and management regimes

Abstract The response of a long‐term, mixed‐species hayfield in Maine, USA, to commercial fertilizers and liquid dairy manure was evaluated over a 6‐year period, including the effects on yield, nutrient concentration and cycling, forage species composition and soil nutrient levels. Nutrient treatments included an unamended control, N fertilizer, NPK fertilizer and liquid dairy manure (LDM). The application rates of plant‐available N, P, and K were constant across treatments. Application of nutrients in any form increased forage yield, generally by 2–4 t dry matter (DM) ha^?1 year^?1. Yield from NPK fertilizer was 0·05–0·25 higher than from LDM, due to differences in N availability. Yield responses to P and K were minimal and there appeared to be no difference between P and K in fertilizer and manure. The forage sward became increasingly dominated by grass species as the experiment progressed; application of P and K in fertilizer or LDM allowed Agropyron repens to increase at the expense of Poa pratensis. Forage nutrient removal accounted for all applied N and K, and nearly all applied P, throughout the study period, demonstrating the important role these forages can play in whole‐farm nutrient management.     

Origin and history of grasslands in Central Europe – a review

In terms of origin, grasslands in Central Europe can be classified into (i) natural grasslands, predetermined by environmental conditions and wild herbivores; (ii) seminatural grasslands, associated with long‐term human activity from the beginning of agriculture during the Mesolithic–Neolithic transition; and (iii) improved (intensive) grasslands, a product of modern agriculture based on sown and highly productive forage grasses and legumes. This review discusses the origin, history and development of grasslands in Central Europe from the Holocene (9500 BC) to recent times, using archaeobotanical (pollen and macroremains), archaeozoological (molluscs, dung beetles, animal bones) and archaeological evidence, together with written and iconographic resources and recent analogies. An indicator of grasslands is the ratio of non‐arboreal/arboreal pollen and the presence of pollen of species such as Plantago lanceolata and Urtica dioica in sediments. Pastures can be indicated by Juniperus communis pollen and charcoal present in sediments and the soil profile. Insect‐pollinated species can be studied using cesspit sediments and pollen (from honey) in vessels in graves. In Central Europe, natural steppe, alluvial grasslands and alpine grasslands occurred before the start of agriculture in the early Neolithic (5500 BC); their area was small, and grassland patches were fragmentary in the forested landscape. Substantial enlargement of grasslands cannot be expected to have occurred before the late Bronze Age. The first scythes come from the 7th–6th century BC; therefore, hay meadows probably did not develop before this time. There is evidence of hay meadows in Central Europe during the Middle Ages, documented by macroremains of Arrhenatherum elatius in sediments, written records and long scythes in archaeological assemblages. Based on macroremains analyses, we conclude that there was generally high diversity of seminatural grasslands in the cultural landscape in the Middle Ages, and individual grassland communities were generally species rich. From the beginning of the agriculture until the 18th century, pastures and pasture forests were dominant sources of forage. Large‐scale enlargement of hay meadows and decline of pastures in many regions occurred from the 18th century. Hay making is associated with enlargement of arable fields and the use of cattle as draught animals for ploughing and soil preparation. The spread of A. elatius in Central Europe was enabled by the decline of grazing management and an increased proportion of hay meadows in the 18th and 19th centuries. In some mountain areas, there are no records of large‐scale deforestation and enlargement of grasslands until the 14th century, and the peak of the agriculturally used area was recorded for the period from the 18th to the first half of the 20th century. Grasslands were converted into arable land during periods of war; conversely, grasslands replaced arable land after the collapse of agriculture in many regions of former communist countries following political regime change in the 1990s. The dynamics of the grassland area reflect the development of human society and the political situation, because grasslands are an integral part of the cultural landscape in Central Europe.     

Preservation of protein in wilted lucerne using formic,sulphuric or trichloroacetic acid

A laboratory-scale experiment was conducted with lucerne (Medicago sativa) to determine the effects of acid treatment on proteolysis during ensiling and during subsequent in vitro ruminal protein incubations. Lucerne [300 g dry matter (DM) kg^?1 forage] was either untreated (control) or treated with sulphuric, formic or trichloroacetic acid (a protein precipitant that stops enzyme activity) at levels sufficient to adjust immediately forage pH to 4·0, then conserved as either silage or hay. Time-course data indicated that non-protein nitrogen (N) formation was 70–90% complete after 1 d of fermentation in the silo. Non-protein N concentrations (g kg^?1 total N) were 177 at ensiling and increased to 567 (control), 426 (sulphuric), 398 (formic) and 263 (trichloroacetic) after 60 d of ensiling. Because non-protein N in silage treated with formic and sulphuric acids was nearly three times greater than that in silage treated with trichloroacetic acid, it is clear that the typical acid treatments only slow proteolysis and do not destroy protease activity during ensiling. The ruminal protein degradation rate of conserved forages was slower than that of fresh-cut forage that was preserved with dry ice immediately after cutting. The degradation rate of all acid-treated forages was similar, indicating a consistent effect on ruminal degradation regardless of method of preservation. There was a clear effect of acid treatment on reducing the rate and extent of ruminal degradation of protein in lucerne hay.     

The effect of grazing management on plant species richness on the Qinghai‐Tibetan Plateau

In Maqu County, Gansu Province, China, there are two types of grazing management. Under multi‐household (MH) management, grassland is jointly managed by two or more households without fences between pastures. Under single‐household (SH) management, fenced‐off parcels of grassland are used. SH management was imposed in the belief that it would alleviate grassland degradation. Comparable land parcels with similar stocking rates subject to MH and SH management were identified and surveyed to determine the species present and species density (m^?2). MH land had greater number values than SH but the differences were not significant. A further analysis indicated that the number of households using MH land influenced species richness. There was also evidence to suggest that the quality of the forage available deteriorated under SH management. It was concluded that MH grazing resulted in greater species richness than SH and is perhaps an important contributor to biodiversity conservation and grassland management for the region.     

Effect of forage species and ensiling conditions on silage composition and quality and the feed choice behaviour of goats

The hypothesis was that forage species and ensiling conditions have an impact on the formation of biogenic amines and the feed choice of goats in short‐term preference trials. At ensiling, lucerne (Medicago sativa L., first cut), red clover (Trifolium pratense L., first cut) and Italian ryegrass (Lolium multiflorum Lam ., second cut) were treated differently to obtain a range of fermentation qualities. Six treatments of each forage species were prepared and included different dry‐matter concentrations, chemical and biological silage additives, and additions of soil. Silages were sampled for chemical analyses (proximate constituents, fermentation products and other volatile compounds, crude protein fractions and biogenic amines) and stored anaerobically in vacuum‐sealed plastic bags for use in preference trials (one trial for each forage species) with Saanen‐type wethers (n = 8 or 6). Each possible two‐way combination of the six silage treatments and a standard hay (n = 21 combinations) was offered for ad libitum intake for 3 hr. Data were analysed using multidimensional scaling, analysis of variance and correlation analysis between silage characteristics and dry‐matter intake (DMI). For each forage species, fermentation characteristics and crude protein fractions revealed only small differences among treatments. Although the degree of proteolysis, as measured by non‐protein nitrogen, of all silages was high, biogenic amine and butyric acid concentrations were low. The different treatments apparently had no direct influence on the formation of biogenic amines and feed choice. The preference behaviour within one forage species was strongly divergent, but DMI rankings of the three species were very similar.     

Cattle select against the invasive grass tall fescue in heterogeneous pastures managed with prescribed fire

Tall fescue (Festuca arundinacea) is a Eurasian forage grass extensively planted in the United States. However, an endophytic fungus in tall fescue, Epichloë coenophiala, causes health problems in cattle. We predicted that cattle prefer to graze alternative forages when available. We also predicted that cattle use tall fescue more intensively in recently burned areas, as fire can increase forage quality. We tested these predictions in four diverse‐forage pastures in Iowa, comparing use by cattle of tall fescue and four alternative forages (non‐fescue cool‐season grasses, native warm‐season grasses, non‐leguminous forbs and legumes) to their availabilities at the pasture scale. We also examined how tall fescue influences the distribution of grazing at a fine scale (0.1‐m² quadrats). Tall fescue was the most abundant forage (46% of plants), but composed only 26% of grazed vegetation. In contrast, legumes composed 12% of available forage but 25% of grazed vegetation. Other forages were used in proportion to availability. At a fine scale, total grazing frequency (proportion of plants grazed) was lower in quadrats containing abundant tall fescue, and higher in quadrats with abundant warm‐season grasses. Grazing frequency of tall fescue and other cool‐season grasses was greatest in recently burned quadrats, but total grazing frequency did not increase after burning. Our results show that although cattle graze tall fescue, particularly following burns, they limit their use of this grass. Given that tall fescue is underused, creates health risks for cattle, and degrades wildlife habitat quality, it may be advisable to reduce tall fescue in pastures.