Increase in DNA fragmentation and the role of ethylene and reactive oxygen species in petal senescence of Osmanthus fragrans

In sweet osmanthus (Osmanthus fragrans Lour.) flowers, petal browning, partial abscission and wilting are the visible symptoms of senescence, 4–5 days after flowering, which seriously affect its ornamental and economic value. In the present study, DNA fragmentation was used as a marker to investigate the role of ethylene and reactive oxygen species (ROS) in flower senescence of O. fragrans ‘Liuye Jingui’. In intact plants, nuclear shrinkage and DNA fragmentation occurred at the late full flowering stage when senescence symptoms became visible. This coincided with a rapid increase in ethylene production, ROS generation and lipid peroxidation. To further determine the role of ethylene and ROS in flower senescence, cut flowers were treated with ethephon, silver thiosulphate (STS), hydrogen peroxide (H₂O₂), and vitamin C (Vc). The vase life of cut flowers was significantly prolonged by 0.2 mM STS and 2.5 mM Vc treatments, but reduced by 0.03% H₂O₂ and 500 mg L⁻¹ ethephon treatments, compared to distilled water. The percentage of DNA fragmentation was dramatically increased by ethephon but reduced by STS treatment throughout vase life. In contrast, the dramatic increase of DNA fragmentation in the H₂O₂ treated samples was only observed at day three, and clear petal abscission and rapid petal wilting occurred only with ethephon. Compared with the distinguishable nuclei and complete vacuoles in both STS treatment and distilled water, ethephon treatment caused substantial damage to large central vacuoles and other organelles, and many petal cells twisted out of shape due to a loss of cytoplasm, resulting in rapid petal wilting. Thus, it is concluded that ethylene plays an important role in flower senescence of sweet osmanthus, enhancing DNA fragmentation, damaging cellular structure, and leading to petal abscission and wilting. In addition, ROS is also involved in the regulation of late DNA degradation and lipid peroxidation.     

A treatment to improve the vase life of cut tulips: Effects on tepal senescence, tepal abscission, leaf yellowing and stem elongation

The vase life of cut tulips (Tulipa spp.) is limited by a combination of leaf yellowing, tepal senescence, and tepal abscission. In many cultivars, moreover, high rates of stem elongation result in stem bending during vase life. In tests with the cvs. Apeldoorn and Frappant, stem bending could be prevented by treatment with ethylene or ethephon. However, these treatments resulted in poor flower opening. The ethephon treatment also resulted in precocious tepal abscission. The negative effect of ethephon on flower opening was overcome by a treatment with gibberellic acid (GA₃). GA₃ also somewhat delayed early leaf yellowing, but did not prevent the early tepal abscission induced by ethephon. The latter problem was overcome by a treatment with benzyladenine (BA). In addition, BA effectively delayed leaf yellowing and also delayed tepal senescence. However, BA produced browning of the lower stem end. This was prevented by the inclusion of calcium ions in the solution. The combination of chemicals (ethephon, GA₃, BA, and calcium ions) was similarly successful in a large number of other tulip cultivars tested. After adaptation of the concentrations the four chemicals were also effective if given as a pulse treatment shortly after harvest.     

Postharvest quality of cut lily flowers

This paper reviews the literature on the postharvest quality of cut lily inflorescences and highlights the need for more information. The main symptoms that limit the length of vase life are abscission of floral buds, lack of flower opening, tepal wilting, and leaf yellowing. Floral bud abscission is regulated by ethylene and can be prevented by treatments with inhibitors of the ethylene receptor. Lack of bud opening is also mimicked by ethylene treatment. It is alleviated by treatment with sugars. These might reduce ethylene effects or act as a source of energy. Depending on the cultivar, tepal wilting is not or is only slightly affected by ethylene. The time to tepal wilting is positively correlated with the levels of endogenous sugars. Leaf yellowing is apparently not affected by ethylene, and is aggravated by the inclusion of sugars in the vase solution. A treatment with hormones, in particular GA₄₊₇ and benzyladenine, can prevent or alleviate the negative effects of sugars on leaf quality.A relatively short period of cold storage often drastically increases the number of floral buds that fail to open. Cold storage also hastens tepal wilting, induces or increases leaf yellowing, and promotes bud abscission. Several lily hybrids, therefore, seem chilling-sensitive. Some of the negative effects of cold storage can be alleviated by sugars, and others by GA₄₊₇, with or without benzyladenine.The lack of ethylene sensitivity of tepal senescence, in several, but not all, cultivars, implies that pollination will also not affect senescence. It would be interesting to test this hypothesis in a few cultivars. It is also not clear if ethylene (or pollination) has an effect on tepal abscission in cut lilies. More data are also needed with regard to chilling injury. Pulsing with sugars might, for example, reduce the degree of injury.     

Postharvest characteristics of cut dahlia flowers with a focus on ethylene and effectiveness of 6-benzylaminopurine treatments in extending vase life

With the aim of extending vase life of cut dahlia flowers, we investigated the postharvest characteristics of the flowers. Our focus was on the role of ethylene on senescence and on treatments that have extended vase life of other flowers. Continuous exposure to ethylene at 2 or 10 μL L⁻¹ significantly accelerated petal abscission in cut flowers. Flowers continuously immersed in 1 or 10 μL L⁻¹ 2-chloroethylphosphonic acid (CEPA) solution wilted earlier than those treated with distilled water (DW) or 0.15 g L⁻¹ citric acid. Ethylene production from the ovary and ray petal was relatively high (4.5 and 0.9 nL g⁻¹ fresh weight h⁻¹, respectively) at harvest, but decreased gradually over 5 days. No remarkable increase in ethylene production was observed during senescence. Silver thiosulfate complex (STS), an inhibitor of ethylene action, did not extend the vase life of cut flowers, although a high silver concentration was detected in flower organs. In contrast, pulse treatment with 1-methylcyclopropene (1-MCP) and dip treatment with 6-benzylaminopurine (BA) extended the vase life of florets, and BA was more effective than 1-MCP when the flowers were held in both DW and CEPA. BA spray treatment extended vase life of cut ‘Kokucho,’ ‘Kamakura’ and ‘Michan’ flowers. These results suggest that dahlia flower senescence is partially regulated by ethylene, and BA is more effective in delaying the senescence of cut dahlia flowers than ethylene action inhibitors.     

Effects of phosphine fumigation on postharvest quality of four Chinese cut flower species

Chrysanthemum (White, Yellow, and Daisy), carnation (Master and Barbara), rose (Carola, Black magic, Diana, Champagne, and Avalanche), and Chinese rose (Golden Medallion, Diplomat, Marina, and Athena) are the main Chinese cut flower species produced for exportation. Cut flowers infested with quarantine pests need methyl bromide (MB) fumigation to satisfy phytosanitary requirements of importing countries. Phosphine (PH₃) is a potential alternative to methyl bromide. Development of phosphine as a phytosanitary treatment requires information regarding its phytotoxicity to cut flowers. Therefore phosphine fumigation at 24 °C and 2 °C was investigated to evaluate its effects on the postharvest quality of cut flowers. Phosphine fumigation for 6 h with dosages as high as 12.2 mg L⁻¹ at 24 °C produced no adverse effects on flower color, diameter, vase life, and other damage indices (DI) for all cultivars. However, different adverse effects on some cultivars were observed after 12 d fumigation at 2 °C. There were significant changes for color values of Carola, Black magic, Diana, Champagne, Avalanche, and Diplomat; significant decrease in flower diameter and vase life of Diana, Champagne, and Avalanche at 3.04 mg L⁻¹, white Chrysanthemum and Diploma at 1.52 and 3.04 mg L⁻¹; significant increase in DI of Champagne and Avalanche at 3.04 mg L⁻¹, and White chrysanthemum, Diana, and Diploma at 1.52 and 3.04 mg L⁻¹. In combination with information on phosphine toxicity to insect pests at ambient and low temperatures in the literature, it is suggested that phosphine fumigation could be a viable replacement of MB fumigation for quarantine treatment of these four cut flower species.     

The effect of CO2 and 1-methylcyclopropene on the regulation of postharvest senescence of mint,Mentha longifolia L.

The regulatory effects of 5 kPa CO₂ and of the ethylene action inhibitor, 1-methylcyclopropene (1-MCP) at 0.5 μmol/l on the senescence of harvested mint, Mentha longifolia L. were assessed. Visual parameters of senescence including yellowing, browning, decay and leaf abscission were recorded and scored on scales linking the onset and progression of senescence to marketability. The effects of plant age on the rate of postharvest senescence and on the efficacy of the CO₂ and 1-MCP treatments were also investigated. All experiments were repeated with and without the presence of exogenous ethylene. Two experimental formats were used, with 6 days storage at room temperature representing local market conditions, and 6 days cold storage at 1.5 °C followed by 4 days at room temperature representing export market conditions. Sprigs from old plants were no longer of marketable quality after 6 days storage at room temperature. Exogenous ethylene accelerated the onset of senescence causing unacceptably high rates of leaf abscission. Raised levels of CO₂ in a controlled atmosphere system were found to be more effective in inhibiting senescence without the presence of exogenous ethylene than pre-treatment with 1-MCP, and no additive effect was found. However in the presence of exogenous ethylene, a combined treatment with 1-MCP together with raised CO₂ levels resulted in a significant additive effect in nullifying the ethylene-induced leaf abscission. Respiration rates as measured by CO₂ production, and ethylene production, were recorded throughout all experiments. While CO₂ levels were not affected by any experimental treatment, ethylene production was elevated in mint sprigs exposed to an initial dose of gaseous 1-MCP, and was further increased under a combined treatment of 1-MCP together with 5 kPa CO₂. However in the presence of exogenous ethylene, CO₂ strongly suppressed the 1-MCP induced ethylene production.     

Sucrose feeding of Cut Dendrobium inflorescences promotes bud opening,inhibits abscission of open flowers,and delays tepal senescence

Inflorescences of Dendrobium cv. Khao Sanan were held in distilled water (controls) or in solutions containing two antimicrobial compounds (silver nitrate and 8-hydroxyquinoline sulfate) with or without 4 g L⁻¹ sucrose or 4 g L⁻¹ glucose. Sugar + antimicrobial compounds promoted bud opening and largely prevented abscission of open flowers. It also resulted in a delay of tepal senescence. No clear effect was found of sucrose feeding on the concentrations of sucrose, glucose and fructose in the tepals of open flowers, but an increase in sucrose, glucose, and fructose concentrations was found in the column + labellum. It is concluded that the effect of sugar feeding on abscission and tepal senescence might relate to a reduction of ethylene sensitivity. It was previously concluded that tepal senescence in Dendrobium flowers was not regulated in the tepals but in the column. It is not clear how the increased in sugar levels in the column + labellum relates to the delay of tepal senescence after sugar feeding.     

Postharvest physiology and volatile production by flowers of Ptilotus nobilis

Ptilotus nobilis (Lindl.) F. Muell. has potential in the floriculture industries as a cut flower crop. Ethylene production and respiration rates, fresh weight changes and volatile scent production from cut inflorescences of P. nobilis cultivars Passion (dark pink flowers) and Purity (white-green flowers) were measured during vase life. Inflorescence weight loss was significant (P < 0.001) during vase life with wilting and colour loss being the primary reasons for loss of vase life. Inflorescences ready for the cut market stored and at 22 °C had vase lives of >12 d. Ethylene production by inflorescences was low to negligible. Treatment with silverthiosulphate (STS) and ethylene had no effects on vase life. Evidently, ethylene did not play a role in determining the postharvest longevity of cut P. nobilis flowers. Respiration rates of inflorescences were high at harvest (>700 mg CO₂ kg⁻¹ FW h⁻¹) and declined gradually thereafter during vase life. Total volatile emissions followed a similar pattern. For Passion, respiration rates of immature florets were significantly greater (P = 0.02) than florets from other developmental stages while the calyx produced the most CO₂. For Purity, respiration rates of florets of different maturities did not differ and the reproductive tissue produced the most CO₂. Only fully opened mature florets with their stigma and anthers revealed, emitted significant quantities of volatiles (P < 0.001) and primarily from the calyx tissue for both cultivars. The individual volatiles differed somewhat for the two cultivars. However, both produced significant quantities of benzaldehyde, 3,5-dimethoxytoluene and benzyl alcohol. These compounds have previously been associated with desirable floral scent.     

Control of postharvest grey mould (Botrytis cinerea Per.: Fr.) on strawberries by glucosinolate-derived allyl-isothiocyanate treatments

The vapours of allyl-isothiocyanate (AITC) were evaluated in in vitro and in vivo trials against Botrytis cinerea, a severe pathogen of strawberries. In in vitro trials AITC activity was assayed on conidial germination and mycelial growth of the fungus. The mycelium appeared less sensitive to AITC than conidia (EC₅₀ values of 1.35 mg L⁻¹ and 0.62 mg L⁻¹, respectively). In addition, AITC had a fungistatic effect against the pathogen, since the values of EC₅₀, for both parameters, increased by around 30% after AITC removal. In in vivo trials, ‘Tecla’ and ‘Monterey’ strawberries (spring-bearing and day-neutral cultivars, respectively) obtained from organic production and naturally infected by B. Cinerea, were exposed for 4 h in an atmosphere enriched by pure AITC or derived from defatted seed meals of Brassica carinata (0.1 mg L⁻¹, in a 0.1 m³ treatment cabinet). After 2 days at 0 °C and another 3–4 days at 20 °C, the fruit were evaluated for grey mould infections. The AITC treatment reduced the decay caused by the pathogen by over 47.4% up to 91.5%, significantly different from the untreated fruit. No significant differences were found between synthetic and glucosinolate-derived AITC. Residue analysis performed on fruit at the end of storage (7 d after treatment) showed values lower than 1 mg kg⁻¹. Total phenolic content and antioxidant capacity estimated in treated and untreated strawberries showed no significant difference between control and AITC treated fruit. Our results show it is possible to reduce the incidence of postharvest grey mould on strawberries with a treatment of AITC (0.1 mg L⁻¹) for 4 h, opening a potential application of biofumigation in the postharvest control of B. cinerea in strawberry.     

Effects of low temperature storage and sucrose pulsing on the vase life of Lilium cv. Brindisi inflorescences

Lilium cv. Brindisi inflorescences were stored at 2.5 °C for 5, 10, 15 or 20 d, comparing dry storage with storage of the stem ends in water. Prior to storage, inflorescences were treated with 20 or 100 g L⁻¹ sucrose in water, for 20 h at 20 °C. After storage the inflorescences were individually placed in water at 20 °C. The floral buds were still closed at the end of cold storage. In experiments carried out in summer, the time to bud opening was hastened by storage at 2.5 °C in water, more so after a longer period of cold storage. The time to tepal senescence after cold storage in water decreased with the time of storage. The time to tepal abscission was about 1 day longer than the time to tepal senescence. Repeat experiments in late fall and winter additionally showed early leaf yellowing after cold storage. Compared to the experiments in summer, more desiccated floral buds were found in the fall. Pulse treatment with 100 g L⁻¹ sucrose prior to cold storage reduced the number of desiccated buds. However, leaf yellowing was aggravated by the 100 g L⁻¹ sucrose pulse treatment. Compared to cold storage in water, dry storage at 2.5 °C further hastened the time to bud opening and also further hastened tepal senescence and abscission. Dry storage also produced more buds that desiccated or opened poorly. Sucrose treatment (100 g L⁻¹) alleviated the effects of dry storage on tepal senescence and bud desiccation. The data showed that lily cv. Brindisi inflorescences are prone to chilling injury, but can be stored, depending on the treatment, for 5–10 d, during most of the year.     

The effects of 1-MCP in cyclodextrin-based nanosponges to improve the vase life of Dianthus caryophyllus cut flowers

The present research investigated the effects of a non-volatile formulation of 1-methylcyclopropene (1-MCP) embedded in different cyclodextrin (CD)-based nanosponges (NSs) to extend the postharvest longevity of an ethylene-sensitive carnation cultivar. Cut flowers of Dianthus caryophyllus L. ‘Idra di Muraglia’ were treated with α- and β-CD-based nanosponge-1-MCP complexes (α- and β-NS complexes) in tap water to achieve two different concentrations of active ingredient (0.25 and 0.5 μL L^?1). Treated flowers were compared to cut stems exposed to equivalent concentrations of volatile 1-MCP as well as a tap water control with or without pure α- and β-NS. Identical nanoporous compounds were applied by perfusion to yield a total of 15 treatments. Twenty-four hours after the treatments were applied, the cut flowers were exposed to exogenous ethylene (1 ± 0.2 μL L^?1) for 24 h. The postharvest carnation flower and leaf quality in addition to ethylene production levels were determined daily (beginning 24 h after treatment). None of the α-NS complex applications statistically improved the vase life of cut flowers; however, β-NS complexes were effective in preventing senescence, reducing ethylene production (measured at nearly nil after 11 d), and maintaining original petal color longer. These results were particularly strong at the lowest concentration (0.25 μL L^?1) of β-NS complex. Overall, this method promoted cut flower longevity (loss of ornamental value after 14.7 d; complete damage at day 18.5) better than the commercial 1-MCP gaseous application method.     

A method for evaluating fruit abscission potential of grapes and cherry tomato clusters

Abscission is an important postharvest factor for table grapes and cluster cherry tomatoes. There are methods to measure the individual fruit removal force but until now there has been no objective method to study the abscission at a whole cluster level. The working principle of the instrument is that a cluster is attached to a rod which is allowed to fall freely for a pre-determined distance; as the rod stops, the momentum of the berries is converted to detachment force applied to the abscission zone of each berry. The abscission potential can be evaluated as a percentage of fruit detached from cluster under given conditions. The instrument was constructed for robust and adjustable experimental use. It is shown that the abscission percentage for both table grapes and cluster cherry tomatoes was proportional to the free-fall distance. By using the instrument, we showed that the abscission potential of cluster cherry tomatoes was low during the first 2 weeks of storage and that it increased thereafter. Storage of cluster cherry tomatoes at 7 °C resulted in significantly less abscission than at 12 °C, as assessed either by manual shaking or by using the instrument. Treatment of grapes with the cytokinin-like compound forchlorfenthuron at 4 mg L⁻¹ during the early stages of fruit development resulted in significant reduction in abscission potential. These results demonstrate that the method can be used as a reliable experimental tool for objective evaluation of abscission potential.     

Effect of tire rubber ash and zinc sulfate on yield and grain zinc and cadmium concentrations of different zinc-deficiency tolerance wheat cultivars under field conditions

The aims of these field experiments were to investigate the effectiveness of soil application of rubber tire ash in comparison with soil and foliar applications of zinc (Zn) sulfate to increase Zn and decrease cadmium (Cd) concentrations in wheat grain. A two-year field experiment was conducted during the 2007–2008 and 2007–2008 growing seasons at Isfahan research field, Iran. Ten different Zn-efficiency bread wheat cultivars (Triticum aestivum L.) commonly cultivated in different parts of Iran were subjected to no Zn fertilizer addition (control), soil application of 40 kg ha⁻¹ ZnSO₄, soil application of 100 (for the first year) and 250 (for the second year) kg ha⁻¹ waste rubber tire ash, foliar application of Zn at the mid tillering stage, and foliar application of Zn at the early anthesis stage. In the foliar application, ZnSO₄ was sprayed at a rate of 0.66 kg Zn/ha. Foliar spray of zinc sulfate at early anthesis, in general, had no significant effect on the yield and grain Cd while significantly increased grain Zn concentrations of most cultivars. On average, the foliar Zn treatment at the mid tillering stage (0.66 kg Zn/ha), decreased the mean grain Cd concentration from 0.032 mg kg⁻¹ in the control treatment to 0.024 mg kg⁻¹. While the grain Zn concentrations of some cultivars increased with soil application of Zn sulfate, they were not affected or even decreased in other cultivars. For most studied wheat cultivars, pre-planting application of rubber tire ash in soil resulted in a significant decrease of grain Cd concentrations. The results show that the effectiveness of soil and foliar application of Zn on yield and grain Zn and Cd concentrations greatly depends on the cultivar. The currently recommended rates of soil applications of Zn to ameliorate Zn deficiency are sufficient to increase grain Zn and decrease grain Cd concentrations in some wheat cultivars, while they do not in the others. In this study, soil application of 250 kg rubber tire ash/ha and foliar spray of 0.66 kg Zn/ha at tillering stage were the most effective treatments to ameliorate Zn deficiency and to increase Zn and decrease Cd concentration in grains of most wheat cultivars.     

Effects of ethylene on volatile emission and fragrance in cut roses: The relationship between fragrance and vase life

The relationship between fragrance and vase life and the role of ethylene on volatile emission in cut rose flowers was investigated. No relationship was observed between the amounts of volatile compounds emitted and vase life when fragrant and non-fragrant rose cultivars were compared. Neither ethylene production nor respiration rate of flowers was directly related with vase life. Volatile production during vase life was differential and independent among volatiles originating from different biosynthetic groups. Ethylene did not play a role in the regulation of volatile emission in rose flowers. Endogenous ethylene production was very low in most of the cultivars and did not show autocatalytic production trends. Volatile emission patterns during vase life did not parallel endogenous ethylene production. Exogenous ethylene exposure had differential effects among all cultivars, regardless of the fragrance of the flower. Fragrant cultivar ‘Osiana’ was highly sensitive to exogenous ethylene, with petals abscising within 24 h of ethylene (1 μL L^?1) exposure while other fragrant cultivars ‘Erin’ and ‘Lovely Dream’ had low ethylene sensitivity. Volatile production was unaffected by exogenous ethylene. The results of this study indicate that volatile emission in cut roses is not regulated by endogenous or exogenous ethylene and occurs independently of petal senescence and/or abscission. These results provide a better understanding of the complexity of volatile emission in rose flowers.     

Impact of a decontamination step with peroxyacetic acid on the shelf-life,sensory quality and nutrient content of grated carrots packed under equilibrium modified atmosphere and stored at 7 °C

Peroxyacetic acid (PAA) is a strong oxidizer and exerts antimicrobial properties. The effect of a decontamination step with 80 and 250 mg L⁻¹ PAA on shelf-life of grated carrots stored under equilibrium modified atmospheric packaging at 7 °C was determined and compared with the shelf-life of unwashed and water-washed carrots. Microbial parameters, including total aerobic plate count, numbers of lactic acid bacteria, Lactobacillae and yeasts, and sensory quality were evaluated. Next to these parameters, atmospheric gas composition, pH and nutrient content were also monitored. The suggested packaging configuration prevented CO₂ accumulation, but at the end of the study anoxic conditions were reached for unwashed carrots and carrots washed with 80 mg L⁻¹ PAA. The microbial shelf-life of water-washed carrots was 4 d based on the yeast count, whereas the flavour was not acceptable after 5 d. The total aerobic plate count and the yeast count determined the shelf-life of carrots treated with 80 mg L⁻¹ PAA on 5 d, whereas the flavour was unacceptable after 7 d. None of the microbial parameters determined the shelf-life of carrots washed with 250 mg L⁻¹ PAA. However, this treatment had already a pronounced adverse effect on the initial sensory quality. Water washing already decreased the content of all individually studied nutrients (−16 to −28%), except for lutein content and the antioxidant capacity. Additional losses after adding PAA on day 0 were found for α-tocopherol and phenols. Regardless of the applied treatment, α- and β-carotene remained stable during storage, whereas ζ-carotene, lutein and α-tocopherol were unstable. The phenol content and the antioxidant capacity of unwashed, water-washed and 80 mg L⁻¹ PAA-treated carrots increased significantly at the end of the storage period, whereas no changes were found in carrots treated with 250 mg L⁻¹ PAA.On the condition that carrots were packed under an adequate EMA, the 80 mg L⁻¹ PAA treatment showed possibilities for extending shelf-life without pronounced effects on nutrient content.     

Essential oils and silver nanoparticles (SNP) as novel agents to extend vase-life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers

The aim of this study was to evaluate the efficacy of silver nanoparticles (SNP) and essential oils as novel antimicrobial agents in extending the vase-life of gerbera (Gerbera jamesonii cv. ‘Dune’) flowers. The vase-life of flowers held in a solution containing 5 mg L⁻¹ SNP plus 6% sucrose was found to be significantly higher than with 8-HQC (8-hydroxyquinoline citrate) or control treatments. However, the vase-life was not different to that of flowers held in similar concentrations of silver nitrate. All gerbera flowers held in SNP solutions showed significantly higher relative fresh weight than the control. Vase-life of gerbera flowers was extended by addition of either 50 or 100 mg L⁻¹ carvacrol and either 1 or 2 mg L⁻¹ SNP from 8.3 to 16 d. In addition, the relative fresh weight and solution uptake of gerbera flowers were increased by addition of 100 mg L⁻¹ essential oils and 1 or 2 mg L⁻¹ SNP as compared to that of control flowers. Our results suggest the potential application of essential oils or SNP as novel alternatives to common chemicals used in preservative solutions for gerbera flowers.     

Treatment with chlorine dioxide extends the vase life of selected cut flowers

The accumulation of bacteria in vase water is often associated with premature senescence in many cut flower species. In the present study, we tested the efficacy of aqueous chlorine dioxide (ClO₂) to extend flower display life by preventing the build-up of bacteria in vase solutions. The addition of 2 or 10 μL L⁻¹ ClO₂ to clean deionized water extended the vase life of Alstroemeria peruviana ‘Senna’, Antirrhinum majus ‘Potomic Pink’, Dianthus caryophyllus ‘Pasha’, Gerbera jamesonii ‘Monarch’, Gypsophila paniculata ‘Crystal’ and ‘Perfecta’, Lilium asiaticum ‘Vermeer’, Matthiola incana ‘Ruby Red’ and Rosa hybrida ‘Charlotte’ flowers by 0.9–13.4 d (7–77%) relative to control (i.e. 0 μL L⁻¹ ClO₂) stems. The beneficial effects of ClO₂ treatment were associated with a reduction in the accumulation of aerobic bacteria in vase water and on cut surfaces of flower stems. ClO₂ treatment was also effective in maintaining or extending the vase life of A. majus ‘Potomic Pink’, Dendrathema × grandiflorum ‘Albatron’, G. paniculata ‘Perfecta’ and M. incana ‘Ruby Red’ flowers even when stems were placed into water containing 10¹¹ CFU L⁻¹ bacteria. The efficacy of 10 μL L⁻¹ ClO₂ in vase water containing 0.2 g L⁻¹ citric acid and 10 g L⁻¹ sucrose to extend the display life of G. jamesonii ‘Lorca’ and ‘Vilassar’ flowers was equal to or greater than other tested biocides (i.e. aluminum sulfate, dichloroisocyanuric acid, 8-hydroxyquinoline sulfate, Physan 20™, sodium hypochlorite). Taken collectively, the results of the present study highlight the potential of aqueous ClO₂ for use as an alternative antibacterial agent in flower vase solutions.     

Vitamin C in broccoli (Brassica oleracea L. var. italica) flower buds as affected by postharvest light,UV-B irradiation and temperature

In this study, the changes in vitamin C, l-ascorbic acid (AA) and l-dehydroascorbic acid (DHA) levels in broccoli flower buds were examined during pre-storage and storage periods, simulating refrigerated transport with wholesale distribution and retail, respectively. Broccoli heads were pre-stored for 4 or 7 days at 0 °C or 4 °C in the dark and then stored for 3 days at 10 °C or 18 °C. During storage the broccoli heads were exposed for 12 h per day to three different levels of visible light (13, 19 or 25 μmol m⁻² s⁻¹) or a combination of visible light (19 μmol m⁻² s⁻¹) and UV-B irradiation (20 kJ m⁻² d⁻¹), or they were stored in the dark. The vitamin C content in broccoli flower buds during storage was significantly affected by pre-storage period and temperature. Higher vitamin C levels in flower buds after storage were observed for broccoli heads pre-stored for 4 days or at 0 °C as compared to those pre-stored for 7 days or at 4 °C. Storage temperature also affected vitamin C in broccoli flower buds, with higher levels observed for broccoli stored at 10 °C than at 18 °C. Hence, vitamin C in broccoli flower buds was demonstrated to decrease together with increasing pre-storage period, pre-storage temperature and storage temperature. AA in broccoli flower buds was influenced mainly by storage temperature and to a minor extent by pre-storage temperature. The DHA level and DHA/AA ratio were stable in flower buds of broccoli pre-stored for 7 days, whereas increasing tendencies for both DHA level and ratio were observed after pre-storage for 4 days. These results indicate a shift in the ascorbate metabolism in broccoli flower buds during storage at low temperatures, with its higher rate observed for broccoli pre-stored for shorter time. There were no effects of the light and UV-B irradiation treatments on vitamin C, AA and DHA levels in broccoli flower buds.     

Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil

In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based on the use of thiabendazole and imazalil. However, these fungicides have been used in a manner highly conducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruit decay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds. Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatum considering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo. One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves (43 isolates) and packing houses (66 isolates). EC₅₀ was determined for each isolate by measuring colony diameters on different agar dilutions of the fungicide. The mean EC₅₀ value of the green mold isolates collected from the groves was 0.14 ± 0.03 mg L⁻¹ while the mean EC₅₀ of those collected from packing houses was 0.13 ± 0.05 mg L⁻¹. No resistant isolates were found in the field where the fungicide is not used, while one isolate originated from a packing house showed an EC₅₀ of 3.40 mg L⁻¹, 26-fold higher than the mean level. This isolate was collected from lemons stored in cool rooms of a packing house where PYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR applied at 500–600 mg L⁻¹ by immersion for 60 s at room temperature to inoculated oranges and mandarins. In contrast, the resistant isolate was not controlled by PYR applied at 1000 mg L⁻¹. Thus, the introduction of PYR applied into packing houses should be done carefully and control strategies should be implemented in order to minimize the development of resistant isolates.     

Effect of treatment with dimethyl dicarbonate on microorganisms and quality of Chinese cabbage

The effects of dimethyl dicarbonate (DMDC) (200 mg L⁻¹) treatment on microorganisms and quality of Chinese cabbage were investigated. The results indicated that in the different tissues treated by DMDC, a significant reduction (P < 0.05) of total aerobic plate count (TAPC) of the leaf (ca. 4.49 log cfu g⁻¹) and stalk (ca. 4.45 log cfu g⁻¹), as well as the count of total yeasts and molds of the leaf (ca. 3.02 log cfu g⁻¹) and stalk (ca. 3.62 log cfu g⁻¹), was obtained in comparison with the control (sterile water dip). However, in the flower bud and/or flower treated by DMDC, the reduction of TAPC (ca. 2.74 log cfu g⁻¹) and counts of total yeasts and molds (ca. 2.26 log cfu g⁻¹) were much lower. DMDC treatment affected appearance and texture early in storage, but an impact on the nutritional composition of Chinese cabbage during storage was not found. Additionally, DMDC treatment significantly (P < 0.05) inhibited the activity of polyphenol oxidase (PPO) and peroxidase (POD) late during storage. Therefore, DMDC is a new and effective alternative for sanitation of Chinese cabbage where a protective measure of appearance quality needs to be adopted.