共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
During 2010 and 2011, typical phytoplasma disease symptoms such as little leaves, phyllody and witches’ brooms were observed on black gram, green gram, long bean, shaggy button weed and sesame plants from different regions of Myanmar. The symptomatic samples were analyzed by PCR using universal phytoplasma primers and characterized by sequencing 16S rRNA, ribosomal protein and translocase protein genes. Based on sequence and phylogenetic analysis of the three genes, the phytoplasmas associated with those plants belonged to members of ‘Candidatus Phytoplasma aurantifolia’. To our knowledge, black gram and shaggy button are new hosts for ‘Ca. P. aurantifolia’. 相似文献
3.
Yusuke Takinami Kensaku Maejima Atsushi Takahashi Takuya Keima Takuya Shiraishi Yukari Okano Ken Komatsu Kenro Oshima Shigetou Namba 《Journal of General Plant Pathology》2013,79(3):209-213
Phytoplasma-induced floral malformations such as virescence, phyllody, and proliferation were observed on hydrangeas in Gunma Prefecture, Japan. Phylogenetic analyses based on 16S rRNA, secY, groEL, and amp gene sequences indicated that the affected hydrangea plants were associated with phytoplasmas belonging to ‘Candidatus Phytoplasma asteris’, but not to ‘Ca. P. japonicum’, which occurs in hydrangeas showing phyllody in Japan. This is the first molecular evidence of an association of ‘Ca. P. asteris’ with hydrangea plants in Japan. 相似文献
4.
Pavšič Jernej Mehle Nataša Nikolić Petra Dermastia Marina 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(4):801-809
A European quarantine organism ‘Candidatus Phytoplasma pyri’ causing devastating pear decline disease has been reported to affect pear trees in several European countries. In this study a multilocus sequence analysis was successfully used to gain detailed insight into the molecular diversity of thirty closely related ‘Candidatus Phytoplasma pyri’ isolates from different orchards in Slovenia. Among three genomic regions analyzed, the 16S/23S rRNA intergenic spacer region was the most conserved among Slovenian isolates with 99.7 % sequence identity, yielding only three distinct genotypes. On the other hand, five different genotypes were detected when analyzing secY and aceF genomic regions that shared sequence identity of 94.8 and 97.2 %, respectively. Six of the detected genotypes, specifically four in the secY region and one in each of the two other analyzed genomic regions, were unique for Slovenia. At least eight different haplotypes were found with multilocus sequence analysis, indicating high molecular diversity among Slovenian ‘Ca. P. pyri’ isolates. Haplotypes were clustered into two major clusters, separated by at least 45 mutations. No connection was established between haplotype occurrence and cultivar type. 相似文献
5.
Molecular detection and identification of phytoplasma associated with pepper witches’ broom in China
Pepper witches’ broom (PWB) disease was observed in a field in Yangling, Shaanxi Province, China. The result of mechanical inoculation test for this disease was negative. Phytoplasma-like bodies were observed in ultrathin sections of petiole tissues of symptomatic samples. 16S rRNA gene and tuf gene of phytoplasma were amplified from the total DNA of symptomatic samples. Phylogeny analysis of the 16S rRNA gene and tuf gene suggested that the pepper witches’ broom associated phytoplasma belongs to the subgroup 16SrI-B, which was confirmed by the RFLP analysis of the 16S rRNA gene. The phytoplasma subgroup 16SrI-B was also detected in the vector Cicadella viridis trapped from the infected field. To our knowledge, this is the first report of 16SrI-B phytoplasma causing pepper witches’ broom in China. 相似文献
6.
Daniela Flôres Isolda Cristina Haas Maria Cristina Canale Ivan Paulo Bedendo 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(2):237-242
Since its arrival in the British Isles in 1845 Phytophthora infestans has remained the most destructive pathogen of potato. In the ensuing period, the British and Irish P. infestans populations have undergone major displacements following the immigration of novel strains. Here we report the re-emergence of the Ib mitochondrial DNA haplotype in the British and Irish P. infestans populations associated with the 6_A1 genotype. Historically associated with the previously panglobally distributed clonal lineage US-1, the Ib haplotype has not been detected (with the exception of a single isolate in the mid 1990s) in the British or Irish P. infestans populations since the early 1980s. The 6_A1 isolates analysed possessed mtDNA Ib, but were otherwise quite unlike US-1, having the Pep allozyme genotype 96/96 and novel RG57 and SSR fingerprints. These genetic characteristics strongly suggest that the appearance of the 6_A1 genotype in these populations has resulted from migration (possibly after a recombination event elsewhere). This study highlights the advantages of utilising a range of different markers in pathogen monitoring. 相似文献
7.
Mahnaz Rashidi Youbert Ghosta Masoud Bahar 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(2):157-159
Russian olive trees (Elaeagnus angustifolia) showing witches’ broom symptoms typical of phytoplasma infection were observed in the Urmia region of Iran. A phytoplasma
named Russian olive witches’ broom phytoplasma (ROWBp-U) was detected from all symptomatic samples by amplification of the
16S rRNA gene and 16S/23S rDNA spacer region using the polymerase chain reaction (PCR) which gave a product of expected length.
DNA from symptomless plants used as a negative control yielded no product. The sequence of the 16S rRNA gene and 16S/23S rDNA
spacer region of ROWBp-U showed 99% similarity with the homologous genes of members of the aster yellows group. We also detected
a phytoplasma in neighboring alfalfa plants (AlWBp-U) showing severe witches’ broom symptoms. An 1107 bp PCR product from
the 16S rRNA gene showed 99% homology with the corresponding product in ROWBp-U, suggesting the presence of the same phytoplasma
actively vectored in the area. Further observations showed that Russian olive trees with typical ROWB symptoms were present
in an orchard near Tehran which is located over 530 km south-east of the original Urmia site. The corresponding sequence of
this phytoplasma (ROWBp-T) showed 99% homology to that of the ROWBp-U. A sequence homology study based on the 16S rRNA gene
and 16S/23S rDNA spacer region of ROWBp-U and other phytoplasmas showed that ROWBp-U is most closely related to the 16SrI
group. To our knowledge, this is the first report of a phytoplasma infection in a member of the Elaeagnaceae. 相似文献
8.
9.
Lia W. Liefting Anna C. Padovan Karen S. Gibb Ross E. Beever Mark T. Andersen Richard D. Newcomb David L. Beck Richard L.S. Forster 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):619-623
Sequence comparisons and phylogenetic analysis of the 16S rRNA genes and the 16S/23S spacer regions of the phytoplasmas associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases revealed minimal nucleotide differences between them resulting in the formation of a monophyletic group. Therefore, along with Australian grapevine yellows, the phytoplasmas associated with Phormium yellow leaf and papaya dieback should also be considered as Candidatus Phytoplasma australiense. 相似文献
10.
Alfaro-Fernández Ana Hernández-Llopis Desamparados Font María Isabel 《European journal of plant pathology / European Foundation for Plant Pathology》2022,164(1):147-147
European Journal of Plant Pathology - 相似文献
11.
Bhaskara Adpangaya Sahana Ahmed Bandalli N. Jawad Adkar-Purushothama Charith R. Prasad Mysore N. Nagendra Sreenivasa Marikunte Y. Maheshwar Pavagada K. Murthy 《植物病害和植物保护杂志》2013,120(5-6):189-193
Journal of Plant Diseases and Protection - The present study reports the development and evaluation of a hemi-nested polymerase chain reaction (hnPCR) assay for the efficient detection of... 相似文献
12.
Cai-Li Lin Tao Zhou Huai-Fang Li Zai-Feng Fan Yong Li Chun-Gen Piao Guo-Zhong Tian 《European journal of plant pathology / European Foundation for Plant Pathology》2009,123(3):321-330
Two plasmids were cloned from paulownia witches’-broom phytoplasma-Nanyang strain (PaWBNy). Southern blotting using pPaWBNy-1ORF1
probe confirmed the existence of the two plasmids. The 4485 bp plasmid, designated pPaWBNy-1, had a nucleotide content of
24 mol% G+C and contained six putative open reading frames (ORFs). The 3837 bp plasmid, designated pPaWBNy-2, had a nucleotide
content of 25.9 mol% G+C and contained five putative ORFs which showed similarity with ORFs in pPaWBNy-1. The two plasmids
contained a series of tandem repeats and encoded a replication-associated protein (RepA) and a single-stranded DNA-binding
protein (SSB), which were necessary for the replication of plasmids. Seven putative proteins encoded by two plasmids were
predicted to contain one or more hydrophobic transmembrane domains, respectively, and presumably to be localised to the membrane.
ORF4 from pPaWBNy-1 was partially cloned and the recombinant protein with His-tag expressed in Escherichia coli. The fusion protein was used for immunisation and the polyclonal antiserum to ORF4 protein detected the native expression
of ORF4 protein in Western blot analysis from infected but not healthy plants. 相似文献
13.
Chinmay Biswas Piyali Dey Subrata Satpathy Manoj Kumar Pratik Satya B. S. Mahapatra 《Phytoparasitica》2013,41(5):539-543
Mesta (Hibiscus sabdariffa) is an important bast fiber crop. In August 2011, there was an outbreak of a phytoplasma-like disease on H. sabdariffa in different villages of the northern coastal mesta-growing region of Andhra Pradesh, India, covering mainly two districts – Srikakulam and Vijayanagaram. The infected plants showed characteristic symptoms such as phyllody and reddening of leaves. PCR with P1/P7 universal primer pair of 16 S rDNA yielded amplicons of 1850 bp from all symptomatic mesta leaf samples similar to samples of brinjal little leaf (phytoplasma positive reference control). However, asymptomatic samples were not amplified. Multiplex nested-PCR showed simultaneous amplification of DNA fragments with phytoplasma specific primers, viz., P1/P7 universal primer pair of 16 S rDNA, nested primer pair R16F2n/R2, uvrB and DegV gene-specific uvrB-degVF/R primer generating amplicons of 1850 bp, 1200 bp and 1023bp, respectively. However, SecY-map gene specific primer SecY-mapF/R was not amplified. The 1023 bp nucleotide sequence of uvrB and DegV gene of the phytoplasma was deposited in the GenBank (NCBI) with the accession no. JX975061. NCBI BLASTn analysis of the 1023 bp products showed that the phytoplasma strain belonged to elm yellows group (16SrV-D). This is the first report that Hibiscus sabdariffa is infected by a phytoplasma and we named it mesta phyllody disease (MPD). 相似文献
14.
Jana Fránová Hana Ludvíková František Paprštein Assunta Bertaccini 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(4):675-688
In several European countries apple trees are affected by apple proliferation disease, which is usually associated with the presence of ‘Candidatus Phytoplasma mali’. During 2010, samples from several apple trees displaying proliferation symptoms were collected throughout the Czech Republic to verify identity of phytoplasmas detected in association with the disease. The majority of the 74 apple trees examined using molecular tools were positive for ‘Ca. P. mali’ presence. The 16S–23S ribosomal genes, the ribosomal protein genes and the nitroreductase and rhodonase like genes were then studied to verify phytoplasma strain variability on multigenic bases. Two RFLP profiles and correspondingly two genetic lineages were found in the PCR-amplified fragments covering the 16S–23S rDNA spacer region. ‘Ca. P. mali’ strains belonging to rpX-A subgroup were identified in the majority of the apple tree sampled, whereas phytoplasmas belonging to the rpX-B subgroup were distributed sporadically. The apple proliferation subtypes AP-15 and AT-2 exhibited nearly equal occurrence; the AT-1 subtype and a mixture of the two or all three of the AP subtypes were infrequently found. The PCR/RFLP results were confirmed by nucleotide sequence analyses of selected ‘Ca. P. mali’ strains. 相似文献
15.
16.
‘Candidatus Liberibacter asiaticus’ (CLas) is associated with the most destructive disease of citrus, huanglongbing (HLB). The most widely used methods for detection of CLas are PCR-based and require purification of DNA from plant samples. Elution of DNA from tissue prints made on nitrocellulose membranes followed by qPCR (TPE-qPCR) was compared to DNA extraction of plant tissue followed by PCR (X-PCR) by testing the same tissue samples. The former estimated a higher CLas population in tissue prints than the latter (t-test; P = 0.009). All extracts prepared for TPE-qPCR throughout the experiment were also tested by conventional PCR and 80.8% were identified as positive. A similar set of stem and petiole tissue samples was tested by TPE-qPCR and immunoassay. Although the detection rate by TPE-qPCR was higher than by immunoassay, about 6% of tissue prints were positive by immunoassay but not by TPE-qPCR. Thus, a higher detection rate would be achieved by combining TPE-qPCR with immunoassay. Significant differences were observed in the performance of nitrocellulose membranes from different manufacturers in these assays. Immunotissue prints showed that the spatial distribution pattern of CLas infection varied widely from one sample to another, but the patterns were highly correlated among serial sections from the same sample, suggesting that CLas preferentially colonizes adjacent phloem cells in a vertical rather than horizontal direction. 相似文献
17.
18.
Zhan-Cui Yu Yang Cao Qun Zhang De-Fa Deng Zhen-Yu Liu 《Journal of General Plant Pathology》2012,78(4):298-300
Chinese scholar tree (Sophora japonica) with witches’ broom symptoms was observed in Shandong Province in China. Phytoplasmas were detected in the diseased plants using 16S rDNA amplification with phytoplasma-specific universal primer pairs. On the basis of the results of 16S rDNA sequencing, virtual restriction fragment length polymorphism patterns and phylogenetic analyses, the phytoplasma found in S. japonica with witches’ broom symptoms was confirmed as a ‘Candidatus Phytoplasma ziziphi’-related strain belonging to the Elm yellows group 16SrV. This is the first report of ‘Ca. P. ziziphi’ infecting S. japonica plant with witches’ broom symptoms. 相似文献
19.
Sjolund M. Jennifer Arnsdorf Yvonne M. Carnegie Mairi Fornefeld Eva Will Torsten 《植物病害和植物保护杂志》2019,126(1):89-92
Journal of Plant Diseases and Protection - Psyllids are small, phloem-feeding insects. Several species are vectors of economically important pathogens, such as ‘Candidatus Liberibacter... 相似文献