Ionic homeostasis and reactive oxygen species control in leaves and xylem sap of two poplars subjected to NaCl stress

We investigated the effects of increasing soil NaCl concentration on intracellular compartmentalization of salt and on the activities of antioxidant enzymes (superoxide dismutase (SOD), ascorbic peroxidase (APX), catalase (CAT) and glutathione reductase (GR)) and their role in the regulation of reactive oxygen species (ROS; O(2)(-*) and H(2)O(2)) in leaves and xylem sap of salt-tolerant Populus euphratica Oliv. and salt-sensitive P. popularis cv. 35-44. Mesophyll cells of P. euphratica exhibited a high capacity for NaCl exclusion and compartmentalization of salt in vacuoles compared with P. popularis. In P. popularis, the salt treatment resulted in large accumulations of Na(+) and Cl(-) in leaves that induced significant increases in O(2)(-*) and H(2)O(2) production despite marked increases in the activities of antioxidant enzymes in leaves and xylem sap. Separation of the isoforms of leaf SOD, APX and CAT by polyacrylamide gel electrophoresis followed by in-gel activity staining revealed that the salt-induced activities of APX and CAT were the result of increases in activities of all the isoenzymes. Leaf injury and shedding of aged leaves occurred following the oxidative burst in P. popularis, indicating that the increased activities of antioxidant enzymes in P. popularis were insufficient to counter the harmful effects of ROS at high soil NaCl concentrations. Unlike P. popularis plants, P. euphratica plants did not exhibit an oxidative burst in response to the NaCl treatments, because of (1) a high salt exclusion capacity and effective compartmentalization of salt in vacuoles, and (2) up-regulation of antioxidant enzymatic activities after the onset of salt stress. We conclude that P. euphratica plants subjected to saline conditions control ROS homeostasis through two pathways: (1) by maintaining cellular ionic homeostasis and thereby limiting the NaCl-induced enhancement of ROS production under long-term saline conditions; and (2) by rapidly up-regulating antioxidant defenses to prevent oxidative damage.     

荒漠河岸林2种典型植物的耐盐性比较

在新疆尉犁县的天然荒漠河岸林里,选择了3个土壤盐度不同的样地,测定了胡杨和柽柳叶片的脯氨酸含量、K+/Na+、氯离子(Cl-)含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量等5个生理指标,并利用模糊数学隶属函数法对二者的耐盐性进行了综合评价.结果表明:随着土壤盐胁迫的增强,胡杨叶片的脯氨酸含量总体上呈增加趋势...     

闽东北海涂土壤理化性状分析

根据闽东北沿海滩涂土壤剖面化验数据，比较分析了该地区海涂土壤的理化性状及相关性，并分析了总盐量与８种主要离子的相关性，结果表明，有机质与全氮、全磷、阳离子代换量存在显著相关性，土壤总盐量与Ｃｌ－、ＳＯ２－４、ＨＣＯ－３、Ｃａ２＋、Ｍｇ２＋、Ｋ＋、Ｎａ＋存在显著的相关性。     

Genotypic variation in drought tolerance of poplar in relation to abscisic acid

We investigated effects of water stress and external abscisic acid (ABA) supply on shoot growth, stomatal conductance and water status in 1-year-old cuttings of a drought-sensitive poplar genotype Populus x euramericana cv. I-214 (Italica) and a drought-tolerant genotype P. 'popularis 35-44' (popularis). Populus popularis was more productive and maintained higher leaf water potentials throughout the drought treatment than cv. Italica. Supply of ABA to the xylem sap caused a greater decline in growth and more leaf abscission in shoots of cv. Italica than in shoots of P. popularis. Immediately after initiation of the drought treatment in P. popularis, the ABA concentration ([ABA]) of the xylem increased rapidly and stomatal conductance declined; however, stomatal conductance had returned to control values by the third day of the drought treatment, coincident with a gradual decline in xylem [ABA]. In contrast, xylem [ABA] of cv. Italica initially increased more slowly than that of P. popularis in response to the drought treatment, but the increase continued for 3 days at which time a tenfold increase in xylem [ABA] was observed that was followed by abscission of more than 40% of the leaves. We conclude that sensitivity of poplar roots to variation in soil water content varies by clone and that a rapid short-term accumulation of ABA in shoots in response to water stress may contribute to drought tolerance.     

胡杨盐诱导基因与盐抑制基因的差减杂交显示研究

基因筛选是基因克隆与遗传转化的基础工作 ,其目的是估测在环境刺激或诱导条件下基因表达水平上的变化。即估测直接或间接表达基因的数目 ,并进一步作为分子标记进行基因分离与克隆。胡杨在盐诱导条件下蛋白质电泳结果显示 ,与对照 (0 % )相比 ,既有增加的条带 ,也有减少的条带 ,客观上证明了盐胁迫关闭了一些基因 (抑制基因 )的转录 ,也开启了一些基因 (诱导基因 )的转录。本研究旨在将这些盐诱导及盐抑制基因筛选出来 ,以便进一步进行抗盐基因的筛选与克隆。1　材料与方法胡杨 2ａ生盆栽实生苗 ,分别用 0 % ,0 5 % ,1 %和 2 %的ＮａＣｌ…     

盐分和水分胁迫对落羽杉幼苗的生长量及营养元素含量的影响

采用温室盆栽试验方法 ,研究了不同土壤水分下盐胁迫对 1年生落羽杉实生苗的生长及营养吸收的影响。试验共 12种处理 ,3种水分水平 (W1 ,淹水 ;W2 、W3,土壤含水量分别为田间持水量的 75 %和 2 5 % ) ,每种水分下设 4种盐分处理 (NaCl含量为土壤干重的 0、0 15 %、0 3%和 0 4 5 % ) ,处理时间为 130d。结果表明 :土壤盐分胁迫下落羽杉幼苗的生长及对营养的吸收发生了显著的变化 ,这种变化又因土壤含水量的不同而不同。落羽杉相对高生长、相对地径生长和生物量增量随着土壤盐分浓度的增加和土壤水分含量的减少而减少。在淹水条件下 ,随着土壤盐分浓度的增加 ,落羽杉幼苗根、茎、叶中全N、全P、全Na,以及叶片中的全Ca、全Fe的含量均随着升高 ,而根、茎中的全Fe、全Ca、全Mg含量差异较小。在W2 水分条件下 ,各种盐分处理都在不同程度上增加了根、茎、叶中全N、全Na、全Ca和全Fe含量 ,茎和叶中全P、全K和全Mg含量 ,而根系中的全P、全K和全Mg含量在盐分浓度较高时有所下降。在W3水分条件下 ,各种土壤盐分不同程度地增加了叶片全N、全P和全K含量 ,茎和叶中全Mg和全Ca含量 ,以及根、茎、叶中全Na含量 ,而茎和根中全N、全P和全K含量 ,根、茎和叶中全Fe含量均随着土壤盐分浓度的增加而下降。     

印度西部5种热带干落叶林中凋落物中生物元素和返回到绿叶中的季节性变化(英文)

本文对印度西部5种热带干落叶林中凋落物里生物元素的季节性变化和它们返回到新鲜叶中季节性变化进行研究。对5个树种:阿拉伯金合欢(Acacia nilotica L.)、金合欢(Acacia leucophloea Roxb.)、柚木(Tectonagrandis L.f.)、绒毛野独活(Miliusa tomentosa Roxb.)和紫矿(Butea mono-sperma Lain.)中凋落物的成熟叶、枝和再生部分的N、P、Ca、Mg、K和Na的浓度,以及老叶和幼叶中浓度间的关系进行调查。对这些树种的总干物质进行记录,并分析它们所含的N、P、Ca、Mg、K和Na的含量。其中阿拉伯金合欢叶子中N的含量最高,而金合欢的叶子里Ca和Mg含量最高。叶中含P最多的是阿托伯金合欢、金合欢和紫矿,而含P最少的是柚木和绒毛野独活。K和Na含量在这些树种中没有明显的区别。观察发现,N、P和K的浓度随季节变化大,但Ca和Mg则不然。唯有钾在所有树种中都是最稳定的,吸收N和P可以用于来年新叶的生长。从老叶和凋落物中吸收的营养会支持新叶的生长,并增加土壤的肥力。图1表4参30。     

胡杨叶片基态渗透值的研究*

胡杨(Populus euphratica Oliv.)是我国西北干旱地区盐渍化土地上的主要造林树种之一。近十年来,我国有些学者通过野外调查、田间试验等方法,对胡杨独特的抗盐性进行了广泛研究,但有关胡杨抗盐性生理机制方面的研究还很少,为了探讨胡杨的抗盐性机理,我们在1989年采用初始质壁分离法,对胡杨叶片的基态渗透值进行了研究。     

杉木枯落物燃烧释放污染物特征及PM2.5成分分析

【目的】探究杉木人工林枯落物在不同燃烧状态时的气态及颗粒态污染物排放特征,并提取、测定细颗粒物(PM 2.5 )污染物的化学成分,揭示林火及林地清理等对杉木枯落物分解、养分循环和大气环境的影响。【方法】分别以杉木人工林枯落物中的叶、枝、皮、干4种主要组分为研究对象,运用自主设计的生物质模拟燃烧系统,采用室内模拟燃烧试验,分析阴燃和明燃2种不同燃烧状态不同枯落物组分释放的气态污染物和PM 2.5 特征及差异。采用德国Elementar元素分析仪,测定PM 2.5 中的碳质成分;采用超声提取-离子色谱和ICP/MS技术,测定PM 2.5 中的水溶性离子和水溶性无机元素。【结果】基于杉木枯落物燃烧时的实时测定,CO 2、CO、NO x 、C x H y 、PM 2.5 的排放因子(EF,单位质量燃料在燃烧后产生的污染物质量)变化范围在阴燃条件下分别是1 001.4~1 364.5、202.3~358.8、0.53~3.09、23.17~53.07、5.11~38.37 g ·kg -1 ,而明燃时分别为1 092.4~1 520.7、115.1~242.6、0.16~1.96、1.21~41.50、2.58~21.07 g ·kg -1 。此外,提取测定PM 2.5 成分的结果表明,颗粒物主要由碳质组分、水溶性离子及少量水溶性无机元素等组成。其中碳质组分含量最大,占50%以上,有机碳(OC)和元素碳(EC)平均质量分数分别为43.29%和12.91%;PM 2.5 中测定出6种阳离子(Li +、Na +、NH + 4、K +、Mg 2+、Ca 2+)和5种阴离子(F -、Cl -、NO 2 -、SO 4 2-、NO - 3),总水溶性离子占10%~33%,阴燃以Cl -、K +、Ca 2+、SO 4 2-为主要组分,明燃以K +、Cl -、SO 4 2-、Na +为主;PM 2.5 中提取测定出16种无机元素,分别为K、Ca、Na、Mg、P、Zn、Al、Cu、As、Ba、Cr、Fe、Mn、Cd、Li、Pb,含量极少,仅占PM 2.5 的0.04%~0.58%,其中K、Ca、Na、Mg、P、Zn为主要元素成分,占总无机元素的96.8%~98.8%。【结论】在不同燃烧状态下,杉木枯落物释放各污染物的排放因子存在显著差异,除CO 2外的其他污染物排放因子在阴燃时显著高于明燃;在同一燃烧状态时,杉木枯落物不同组分的CO、CO 2、NO x 、C x H y 和PM 2.5 等污染物的排放因子也存在显著差异。杉木枯落物燃烧排放的PM 2.5 主要由碳质组分、水溶性离子及少量无机元素等成分组成,阴燃时的各成分排放因子整体表现为高于明燃时。     

Nutrient content of standing crop and biological cycling in Pinus patula ecosystem

Nutrient contents were estimated in different components of Pinus patula trees growing in six stands ranging from 8 to 34 years of age in the Darjeeling hills, India. The highest concentrations of most nutrients were found in the leaves but the maximum nutrient pool was contained in the bole which accounted for about 58 to 85% of the total aerial contents of different elements. Among the nutrients, N concentration (2.2%) was the highest, followed by Ca Mg K P Na. Nutrient contents in the standing crop increased with stand age and were nearly 2102 kg Ca, 1911 kg N, 875 kg Mg, 478 kg K, 285 kg P and 82 kg Na per ha in above-ground biomass of the 34-year-old stand. The annual uptake of N is highest but its accumulation in the standing stock is lower than that of Ca in the 34-year-old stand. The uptake and storage of Mg closely resembled those of K. Nutrient return and release were also determined to establish biological cycling in the 34-year-old P. patula ecosystem. Litter is resistent to decay and the forest floor retained a very rich nutrient store over the mineral soil. Turnover rates and times for nutrient fluxes showed the higher efficiency of the return pathway and the greater stability of the soil pool.     

Effects of an exotic conifer (Pinus radiata) plantation on forest nutrient cycling in southeastern Australia

The distribution and movement of N, P, K, Na, Mg, and Ca were studied in southeastern Australia in a 37—year-old Pinus radiata plantation and in a nearby Eucalyptus obliqua — Eucalyptus dives forest of the same age and of the same type as that which had been replaced by the P. radiata plantation. The soil beneath the P. radiata plantation contained significantly less total N and exchangeable K, Mg, and Ca than that beneath the eucalypt forest. No large accumulation of nutrients was found in either the litter or the trees in the P. radiata plantation relative to that in the eucalypt forest. However, there was a slightly greater accumulation of N and K in the P. radiata biomass than in the eucalypt biomass. The annual soil nutrient balance obtained by subtracting outputs (mineral soil leachate + biomass incorporation) from inputs (precipitation + mineral weathering) indicated a more favourable balance for each nutrient in the soil beneath the eucalypts than in the soil beneath the pines. Calculations suggested that these balances could only partially account for the differences in soil nutrient quantities between eucalypt and pine ecosystems. It was hypothesized that these differences are also partially explainable in terms of the nutrient losses accompanying two fires which had occurred in the pine plantation area. Nitrogen balances in this study were incomplete because several potentially important fluxes were not measured.     

Effects of aluminum on growth and nutrient status of Douglas-fir seedlings grown in culture solution

Effects of Al on growth, nutrient uptake and proton efflux were studied in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings grown for about nine months in culture solutions with a pH between 3.4 and 3.6 and with both calcium and magnesium (Ca + Mg) at a concentration of 0.1, 0.5 or 2.5 mM. In the absence of Al, plant dry matter production and root development increased with increasing concentrations of (Ca + Mg) in the culture solution. At the low and intermediate (Ca + Mg) concentrations, optimal root and shoot development were observed at an Al concentration of 4 mg l(-1). At the highest (Ca + Mg) concentration, Al up to 4 mg l(-1) did not affect growth, but at higher concentrations, it significantly reduced both shoot and root growth. As the concentration of (Ca + Mg) in the nutrient solution increased, the concentrations of Ca and Mg increased in shoots and roots also. The concentrations of Ca and Mg in the roots were unaffected by the presence of Al, whereas in the shoots they were either unaffected, or increased, by Al. Concentrations of Al in, or on, roots, or in shoots, did not change in response to changing concentrations of Ca + Mg in the nutrient medium. In general, concentrations of P and K in shoots and roots were higher in seedlings grown in nutrient solutions containing Al. Stimulation of growth by moderate concentrations of Al, which was observed at suboptimal (Ca + Mg) concentrations, was associated with a low NH(4) preference and a low root proton efflux. The Al-induced increase in internal K concentration and reduction in NH(4) preference may be explained by a lower efflux of K and NO(3), respectively.     

植物耐盐机理与耐盐植物选育研究进展*

文中结合对植物耐盐生理基础的讨论，综述了耐盐植物，尤其是耐盐树木的选育研究进展。植物对盐分胁迫的反应和适应是一个复杂的生理过程，既有蛋白质、核酸、碳水化合物等结构和能量物质的代谢，还有酶、激素等生长调节物质的合成与激话。在这一系列的反应过程中包含着离子交换与逆向运输，信号刺激与传递．基因活化与合成，其中渗透调节起着关键作用；在渗透调节过程中，离子如K^ 、Ca^2 等在浓度和时空上的变化对渗透调节的启动，调节速率和调节能力都有着重要作用。此外，脯氨酸、脯氨酸甜菜碱、甘氨酸甜菜碱等作为可配伍溶质在调节渗透势变化、整合盐分离子方面有着无可代替的作用。Ca^2 |作为第二信使的一个重要组成部分．在胁迫信号传递方面的功能正受到日益重视。激素如ABA在渗透调节和胁迫基因的诱导方面发挥了重要作用。胁迫基因目前主要是指渗透调节基因。首先在细菌中被发现，后来在高等植物中也相继分离克隆出来。有许多不同的方法用来选育耐盐植物，包括选、引、育等常规育种方法和基因工程，突变体育种等生物工程方法。它们各有特点．都发挥了各自的作用。对植物耐盐机理有限的认识以及高等植物结构与功能的高度复杂性制约着耐盐植物选育工作的进展。     

本溪山区森林枯落物对水质的影响

以本溪山区主要水源涵养林红松、落叶松、蒙古栎、水曲柳、核桃楸纯林为研究对象,运用定位观测法,对5种类型林分的林外雨与枯落物渗透雨(枯透雨)的水质进行了研究,以及利用5种类型林分之间枯落物渗透水的水质对比,测定林外降雨及各林分枯落物渗透水的pH值、硝酸盐(以N计)、氨氮(以N计)、硫酸盐(以SO_4~(2-)计)、全氮(TN)、全磷(TP)、氟离子(F~-)、氯离子(Cl~-)、钙(Ca~(2+))、钠(Na~+)、镁(Mg~(2+))、钾(K~+)等12种指标,结果表明:大量化学元素和离子通过枯落物后均显著增加,其中变幅最大的K~+,浓度为4.19mg·L~(-1),是林外雨20倍。林外雨各指标浓度依次为SO_4~(2-)TNCa~(2+)Cl~-Na~+NH_3-NNO_3~-NMg~(2+)K~+F~-TP;枯透雨各指标浓度依次为SO_4~(2-)Ca~(2+)TNNO_3~-NCl~-K~+Mg~(2+)NH_3-NTPF~-Na~+。枯透雨中SO_4~(2-)浓度最高,为12.59mg L~(-1);TP浓度最小,为0.25mg L~(-1)。5种纯林在调查期间林外雨和枯透雨的pH趋于中性,而在经过枯枝落叶层的淋溶后,有明显的酸化现象,pH范围在5.1~6.6。     

小胡杨对滨海苏打盐渍土改良效果研究

滨海苏打盐渍土修筑台田后种植小胡杨可以成活,18年后小胡杨林分基本郁闭。以未种植树木的台田作为对照(CK),对小胡杨林地内植被演替规律、土壤水分物理性质进行研究。结果表明,小胡杨成林后,林地内植被向良性方向发展,群落生态型由中度耐盐群落演替成轻度耐盐群落,群落高度也由0.2～0.6 m增加到0.5～0.8m,盖度则达到80%～90%,土壤也由苏打盐渍土转变成轻盐碱土;小胡杨可显著降低土壤容重,改善土壤孔隙状况,增加土壤持水性能,使土壤水分物理性质得到显著改良,其中,对0～10 cm土层的改良效果要好于10～30 cm土层。     

塔里木河河岸带胡杨种群数量特征与空间分布格局

在塔里木河上游垂直河岸设置样带,研究胡杨种群数量特征与空间格局沿地下水位梯度的动态及相互关系,探讨其生存发展的合理生态地下水位及胁迫水位。结果表明:胡杨具有伴河生长的特性,在距河道100m以内胡杨以幼苗为主,种群属进展型;随着离河道距离的增加,胡杨种群幼龄比例减少,胸径逐渐增大,远离河道区老龄个体占优势,种群结构呈倒金字塔形,趋向衰退。胡杨种群密度、幼苗比例、长势与林分郁闭度随离河道距离增加而降低,平均胸径、最大胸径与树冠疏失度则增加;密度、郁闭度与离河道距离(地下水位)呈显著负相关,平均胸径与离河道距离呈正相关。河岸带胡杨空间格局主要呈聚集分布,近河岸区则呈随机分布,随距离增加空间格局转为聚集分布,这是种群集群抵御干旱环境而采取的生态适应对策。地下水位是影响河岸带胡杨种群生长与分布的主要因素,而维系河岸带胡杨种群正常生长的合理地下水位应4.1m,胁迫临界地下水位为5.5m。     

Response of Populus x canescens （Populus tremula x alba） to high concentration of NaCl stress

Introduction Salinity is a major factor in inhibiting plant growth and decreasing forest productivity. Up to 1997, the total area affected by salinity in the world had amounted to 930 million hm2 (FAO), and is still increasing. A global study of land use …     

Mineral nutrient fluxes in rainfall and throughfall in a lowland Atlantic rainforest in southern Brazil

The ecosystems occurring on dystrophic soils, such as sandy soils, are highly dependent on nutrients from the atmosphere and those cycled by their own biota. Nutrient inputs from rainfall and throughfall were measured between October 2001 and August 2003 in a secondary Atlantic rainforest in southern Brazil. Canopy interception (rainfall minus throughfall) was 17.3% of the annual rainfall of 2,235 mm. Monthly interception ranged from 12 to 31% during the rainiest months (precipitation above 200 mm) and from 1 to 45% during the driest months (precipitation below 50 mm) indicating relatively high variability during this period. The studied site may be susceptible to water stress in this period due to the high permeability of the sandy soil. Approximately 80% of the Ca and Na and 57% of Mg were mainly from rainfall (bulk deposition) whereas the main input source for K was net throughfall (about 78%). Mean annual inputs via throughfall (in kg ha⁻¹) were: 90.6 for Na, 29.1 for K, 7.1 for Ca, and 2.9 for Mg. The highest nutrient inputs occurred during the rainy season. Na fluxes were relatively high, while K, Ca, and Mg inputs were low, compared with other tropical and subtropical forests. Information on nutrient fluxes for different forest ecosystems are fundamental for building up a database that can give support to environmental diagnosis, to forest management, and to conservation and restoration techniques.     

不同土壤水分含量下落羽杉根、茎、叶营养水平的差异

应用温室盆栽试验方法,采用完全随机试验设计,研究了土壤水分含量对1年生落羽杉实生苗的营养吸收及分配的影响。试验共有5种处理,即W1(淹水,水深为土壤表面以上5cm)、W2(渍水,水面与土面保持水平)、W3、W4、W5(土壤含水量分别为土壤田间持水量的75%、50%和25%),处理时间为130d。研究结果表明:(1)随着土壤水分含量的减少,落羽杉根、茎和叶中全N质量浓度均逐渐升高,根、茎、叶中全P质量浓度则又先升高后下降的趋势,以W3水分处理最高,根、茎和叶中全Ca、全K、全Na、全Mg、全Fe质量浓度则有不同的变化趋势;(2)所有处理根、茎、叶中全Fe、全N、全P、全Mg、全Na、全Ca、全K质量浓度大小的顺序为叶>根>茎;(3)随着土壤水分含量的减少,落羽杉对各种营养元素的吸收累积量减少,分配到根和茎中的比例增加,分配到叶中的比例逐渐减少。