Evaluation of effects of transgenic Bt maize on microarthropods in a European multi-site experiment

The effects of maize expressing the Bacillus thuringiensis Cry1Ab protein (Bt maize) on soil microarthropods were assessed in the field at four European locations (two in Denmark and two in France) that differ in their climatic conditions or soil properties. Each site was considered as a separate experiment, with separate statistical comparison. Effects of farming practices using Bt maize were compared with conventional farming practices using near-isogenic non-Bt maize and also (at some of the sites) other conventional varieties. Furthermore, at one field site (Foulum, Denmark), the effects of Bt crops were studied in both conventional tillage and reduced tillage contexts. At another field site (Askov, Denmark), Bt maize effects were also compared to the effects of the chemical insecticide dimethoate. Moreover, at three of the field sites (all except Narbons, France), the possibility of a localised Bt effect around the rhizosphere compared to the bulk soil was assessed by sampling within and between maize rows. There were some significant negative effects of Bt maize on microarthropods in soils with a high clay content. Significant differences of the same magnitude also occurred between different conventional varieties of maize, but the effect of dimethoate appeared clearly greater than Bt effects. It is thus debatable if the Bt maize effect is an effect of the Bt toxin or just an effect of the maize variety. Based on the results, it can be concluded that the effect of Bt maize on soil microarthropods was small and within the normal variation expected in conventional agricultural systems.     

Hierarchical classification of environmental factors and agricultural practices affecting soil fauna under cropping systems using Bt maize

The population dynamics of soil organisms under agricultural field conditions are influenced by many factors, such as pedology and climate, but also farming practices such as crop type, tillage and the use of pesticides. To assess the real effects of farming practices on soil organisms it is necessary to rank the influence of all of these parameters. Bt maize (Zea mays L.), as a crop recently introduced into farming practices, is a genetically modified maize with the Cry1Ab gene which produces a protein toxic to specific lepidopteran insect pests. To assess the effects of Bt maize on non-target soil organisms, we conducted research at a field site in Foulum (Denmark) with a loamy sand soil containing 6.4% organic matter. The study focused on populations of springtails (Collembola) and earthworms (Oligochaeta) from samples taken at the beginning and at the end of the maize crop-growing season during 2 consecutive years. Farming practices, soil parameters, the biological structure of soil communities, and the type and age of the crop at the time of sampling, were used as attributes to predict the total abundance of springtails and biomass of earthworms in general and the abundance or biomass for specific functional groups (epigeic, endogeic and anecic groups for earthworms, and eu-, eu to hemi-, hemi-, hemi to epi- and epiedaphic groups for Collembola). Predictive models were built with data mining tools, such as regression trees that predict the value of a dependent variable from a set of independent variables. Regression trees were constructed with the data mining system M5′. The models were evaluated by qualitative and quantitative measures of performance and two models were selected for further interpretation: anecic worms and hemi-epiedaphic Collembola. The anecic worms (r²=0.83) showed preferences for less clay and more silt soil with medium pH but were not influenced directly by farming practices. The biomass of earthworms was greater in early autumn than in spring or late autumn. Biomass of hemi-epiedaphic Collembola (r²=0.59) increased at the end of the maize growing season, while higher organic matter content and pH tended to increase their biomass in spring. Greater abundance of Collembola was also noted in early autumn if the crop was non-Bt maize. The models assessed by this research did not find any effects of the Bt maize cropping system on functional groups of soil fauna.     

Agricultural studies of GM maize and the field experimental infrastructure of ECOGEN

Within the ECOGEN project, long-term field experiments with genetically modified maize, Zea mays L. were conducted to study agro-ecological effects on the soil fauna and agro-economic implications of the technology. Here, we describe the study-sites, experimental layout and results of agro-economic relevance. Experiments were conducted during 2002–2005 in Denmark (Foulum), northern France (Varois) and the Midi-Pyrenees region of southern France (Narbons). MON810 Bacillus thuringiensis (Bt)-varieties expressing the Cry1Ab protein, and a T25 glufosinate-ammonium (Basta) tolerant variety expressing the pat-gene encoding phosphinotrinacetyl-transferase were compared with near-isogenic non-Bt varieties, and conventional maize varieties. At Foulum, the maize was harvested for silage. There were no significant differences in yield between Bt-maize and a near-isogenic non-Bt variety, while a small difference in N-concentration of dry matter was detected in 1 year in a range of a measured quality parameters. Similar yield and quality were found in ploughed and reduced tillage treatments in all varieties. At Varois, the maize was harvested at ripeness and no significant differences in grain yield between Bt-maize and near-isogenic non-Bt varieties were found. These results were expected, as only Narbons harbours significant corn-borer populations. At Narbons, the number of Sesamia and Ostrinia corn-borer larvae were significantly lower in the Bt-maize than in a near-isogenic non-Bt variety and for Sesamia even less than in conventional varieties sprayed with pesticides to control corn-borer infestation. Here, Bt-maize produced a higher grain yield and grain size than a near-isogenic non-Bt variety or allowed a significant reduction in pesticide use. Concentrations of Cry1Ab in the Bt-varieties were sufficient to effectively control corn-borer larvae. In soil, Cry1Ab was close to the limit of detection and the protein did not accumulate in the soil year on year.     

Microbial and microfaunal community structure in cropping systems with genetically modified plants

Soils from field sites at Foulum (DK), Narbons (FR) and Varois (FR) planted with genetically modified maize expressing either the insecticidal Bacillus thuringiensis protein (Bt) or herbicide tolerance (HT), as described elsewhere in this volume, were analysed for nematodes, protozoa and microbial community structure. These analyses were mirrored in single-species testing and in mesocosm experiments, and were coordinated with field samples taken for microarthropods, enchytraeids and earthworms so allowing for cross-comparison and a better understanding of the results observed in the field. Over the first 2 years of the field experiments (in 2002 and 2003), the effect of Bt-maize was within the normal variation expected in these agricultural systems. Sampling in 2004 and 2005 was expanded to include the effects of tillage (i.e. reduced tillage versus conventional tillage) and also the use of HT-maize. Tillage had major effects regardless of soil type (Varois or Foulum), with reduced-tillage plots having a greater abundance of microfauna and a different microbial community structure (measured both by phospholipid fatty-acid analysis (PLFA) and by community-level physiological profiling (CLPP)) from conventionally tilled plots. Grass, as a contrasting cropping system to maize, also had an effect regardless of soil type and resulted in greater microfaunal abundance and an altered microbial community structure. Differences in crop management, which for the Bt-maize was removal of the insecticide used to control European corn borer and for HT-maize was a change in herbicide formulation, were only tested at single sites. There were differences in microbial community structure (CLPP but not PLFA) and sporadic increases in protozoan abundance under the Bt-crop management. The HT-maize cropping system, which covered a shorter period and only one site, showed little change from the conventional system other than an altered microbial community structure (as measured by PLFA only) at the final harvest. The Bt-trait had a minimal impact, with fewer amoebae at Foulum in May 2003, fewer nematodes at Foulum in May 2004 but more protozoa at Varois in October 2002 and an altered microbial community structure (PLFA) at Foulum in August 2005. These were not persistent effects and could not be distinguished from varietal effects. Based on the field evaluations of microfauna and microorganisms, we conclude that there were no soil ecological consequences for these communities associated with the use of Bt- or HT-maize in place of conventional varieties. Other land management options, such as tillage, crop type and pest management regime, had significantly larger effects on the biology of the soil than the type of maize grown.     

The role of laboratory, glasshouse and field scale experiments in understanding the interactions between genetically modified crops and soil ecosystems: A review of the ECOGEN project

The interactions of genetically modified (GM) crops with soil species and ecosystems is complex, requiring both specific and broad spectrum assessments. In the ECOGEN project we undertook experiments at three scales of increasing complexity, using Bt maize expressing the Cry1Ab protein from Bacillus thuringiensis as an example. Test species were selected for laboratory-scale experiments to represent taxonomic groups that we could also monitor at glasshouse and field scales (e.g., nematodes, protozoa, micro-arthropods, earthworms, and snails). In the laboratory, single species were exposed to purified Cry1Ab protein or to Bt maize leaf powder incorporated into simplified diets under controlled conditions. In the glasshouse, multiple test species and soil microbial communities taken from ECOGEN's field sites were exposed to Bt maize plants growing under glasshouse or mesocosm conditions. In the field, evaluations were conducted on our selected indicator groups over multiple sites and growing seasons. Field evaluation included assessment of effects due to the local environment, crop type, seasonal variation and conventional crop management practice (tillage and pesticide use), which cannot be assessed in the glasshouse. No direct effects of Cry1Ab protein or Bt leaf residues were detected on our laboratory test organisms, but some significant effects were detected in the glasshouse. Total nematode and protozoan numbers increased in field soil under Bt maize relative to conventional maize, whilst microbial community structure and activity were unaffected. Field results for the abundance of nematodes and protozoa showed some negative effects of Bt maize, thus contradicting the glasshouse results. However, these negative results were specific to particular field sites and sampling times and therefore were transient. Taking the overall variation found in maize ecosystems at different sites into account, any negative effects of Bt maize at field scale were judged to be indirect and no greater than the impacts of crop type, tillage and pesticide use. Although the ECOGEN results were not predictive between the three experimental scales, we propose that they have value when used with feedback loops between the scales. This holistic approach can used to address questions raised by results from any level of experimentation and also for putting GM crop risk:benefit into context with current agricultural practices in regionally differing agro-ecosystems.     

轮耕对渭北旱塬春玉米田土壤理化性状和产量的影响

为了揭示不同轮耕处理对渭北旱塬春玉米田土壤理化性状及春玉米产量的影响,于2008—2010年在陕西合阳设置了免耕深松免耕(NT/ST/NT)、深松翻耕深松(ST/CT/ST)、翻耕免耕翻耕(CT/NT/CT)、免耕免耕免耕(NT/NT/NT)、深松深松深松(ST/ST/ST)和翻耕翻耕翻耕(CT/CT/CT)6种轮耕模式,测定和分析了各轮耕处理下土壤容重、土壤养分与玉米产量差异。结果表明:(1)各轮耕处理降低了土壤容重,提高了土壤孔隙度,增加了田间持水量,且以NT/ST/NT处理效果最佳;与对照CT/CT/CT相比,NT/ST/NT处理0～20cm和20～40 cm土层土壤容重分别降低11.43%和9.79%,土壤孔隙度分别增加11.05%和9.87%。(2)NT/ST/NT处理对耕层0～20 cm土层土壤有机质和全氮含量影响显著(P<0.05),0～60 cm土层土壤有机质平均含量最高(10.36 g.kg 1),土壤全氮含量平均值比试验开始前和对照CT/CT/CT分别提高10.65%和4.31%;各耕作处理对0～20 cm土层土壤全氮和碱解氮含量影响较大,而对20～40 cm土层土壤有效磷和速效钾含量影响较大,保护性轮耕处理对土壤培肥效应显著(P<0.05)高于传统翻耕处理。(3)NT/ST/NT、ST/CT/ST、CT/NT/CT、NT/NT/NT和ST/ST/ST处理产量比对照CT/CT/CT处理分别增产22.42%、16.33%、3.77%、9.91%和14.18%(P<0.05),以NT/ST/NT处理春玉米增产率最高。     

Effects of conventionally bred and <Emphasis Type="BoldItalic">Bacillus thuringiensis</Emphasis> (<Emphasis Type="BoldItalic">Bt</Emphasis>) maize varieties on soil microbial biomass and activity

Genetically modified (GM) maize containing genes from the soil bacterium Bacillus thuringiensis (Bt) was cultivated on 29% of the total maize production area worldwide in 2009. Most studies to date compare Bt-maize varieties with their near isogenic lines; however, there is little information on the variability of conventional maize breeding lines and how the effects of Bt varieties are ranked within. In our study on the potential risks of Bt-maize varieties, we analyzed tissue quality and compared the effects of ten conventional and GM maize varieties on soil microbiological properties in a replicated climate chamber experiment. All maize varieties were cultivated twice in the same soil microcosm. Shoot yields and soluble C in leaf tissue of Bt varieties were higher than the ones of non-Bt. Soil dehydrogenase activity was reduced by 5% under Bt varieties compared to non-Bt, while most of the other soil microbial properties (soil microbial biomass, basal respiration) showed no significant differences between Bt and non-Bt varieties. The leaves and roots of one Bt variety were decomposed to a greater extent than the ones of its near isogenic line; the conventional breeding lines also showed higher values. Changes in crop and soil parameters were found when comparing the first and the second crops, but the effects of repeated cropping were the same for all tested varieties. For the studied parameters, the variation among non-Bt-maize varieties was similar to the difference between Bt and non-Bt varieties.     

Earthworms and rate of breakdown of soybean and maize residues in soil

A field survey was conducted to determine the numbers and biomass of earthworms in soils receiving different tillage and cropping treatments, and to investigate in a greenhouse study the effect of earthworms on the rate of breakdown of soybean (Glycine max) and maize (Zea mays L.) residues. The numbers and biomass of earthworms under continuous soybeans were greater than those present under maize, possibly due to the adverse effects of insecticide and anhydrous ammonia fertilizer used with maize. No-tillage doubled the population of earthworms under soybeans, when compared with ploughing. Numbers (141 m^?2) and biomass (26.5 g m^?2) of earthworms under no-till soybeans were still much lower than the numbers (1298 m^?2) and biomass (224.5 g m^?2) under pasture receiving heavy applications of animal manure. Using 16-1 pots in the greenhouse, the effect of 0, 15 (250m^?2) and 30 (500m^?2) earthworms (Lumbricus rubellus) pot^?1 on the rate of breakdown during 54 days of 50 g of soybean or maize residues in the Raub silt loam (Aquic Argiudoll) was studied. At 36 days, 60% of the soybean residues were recovered from pots to which no earthworms had been added, whereas in the presence of earthworms, only 34% of the soybean residues remained. In the absence of earthworms, 85% of the maize residues were recovered at 36 days, compared with only 52% in the presence of earthworms. At 36 days, 48% of the original maize residues added were still > 2 mm in length in the absence of earthworms, whereas only 26% were > 2 mm in length in the presence of earthworms. Earthworms also increased the aggregate stability of the Raub soil, when determined on moist (19–22% w/w) samples, but had no effect on soil water retention at ?33 and ?1500 kPa. The possible implications of greater earthworm activity on increasing residue incorporation and breakdown and subsequent effects on soil temperatures under no-till maize production are also discussed.     

Long-term impact of no-till conservation agriculture on abundance and order diversity of soil macrofauna in continuous maize monocropping system

Unsuitable soil management in agriculture is known to results in the deterioration of soil health and the decline of biodiversity. The experiment tested whether no-tillage with mulch (NT), could boost biological activity of soil macrofauna population in continuous maize monocropping system compared with rotational tillage with mulch (RT) and conventional tillage (CT). Soil macrofauna was sampled at the end of the 2014/2015 growing season using 25?×?25?×?25?cm steel monoliths. The mean density of individual orders was higher (p?p?相似文献   

Nutrient concentration and yield of maize (Zea mays L.) after vetch (Vicia sativa L.) in conventional and reduced tillage systems

The effects of tillage on plant availability nutrients are critical to develop nutrient management strategies to optimize yield. The objective of this study was to evaluate differences in the concentration of 19-nutrient and yield after 4-year of conventional [moldboard plough (MT)] and two reduced [rototiller (ST) and chisel (CT)] tillage systems in maize (Zea mays L.) after winter vetch (Vicia sativa L.) in double crop one year. Three tillage systems were compared from 2005 to 2008 in area located in the western region of Turkey under semi-arid Mediterranean clay-loam soils. Nutrients were determined in maize leaves, stems, roots and soils. Results show that the concentration of macronutrients were found to be higher in leaves and stems of maize than roots in three tillage systems of all years, while the opposite was true for micronutrients. Among the macro and micro-nutrients, there was no effect of tillage on nutrient concentration in all maize tissues for sulfur, magnesium, sodium and copper (S, Mg, Na and Cu). However, the nitrogen, potassium, calcium, boron, zinc, manganese, iron, aluminum, barium, cadmium, cobalt, chromium, nickel, lead and selenium (N, K, Ca, B, Zn, Mn, Fe, Al, Ba, Cd, Co, Cr, Ni, Pb and Se) were affected by tillage. ST increased N in stems of 2006 compared with other systems. K in roots of 2006 was 52 and 30% greater in CT than in ST and MT, respectively, while ST and MT of 2007 resulted in 38 and 41% greater than CT. In two of four years, ST contributed to higher grain yield compared with other systems. In general, ST can effectively contribute to increase maize yield following winter vetch compared with MT under this region. Results suggested the need for different management systems associated with reduced tillage including rotation, particularly for basic nutrients. Further, results showed similarities and differences with other studies under tillage with maize following winter vetch.     

Decomposition processes under Bt (Bacillus thuringiensis) maize: Results of a multi-site experiment

The effects of maize expressing the Bacillus thuringiensis Cry1Ab protein (Bt maize) on decomposition processes under three different European climatic conditions were assessed in the field. Farming practices using Bt maize were compared with conventional farming practices using near-isogenic non-Bt maize lines under realistic agricultural practices. The litter-bag method was used to study litter decomposition and nitrogen mineralization dynamics of wheat straw. After 4 months incubation in the field, decomposition and mineralization were mainly influenced by climatic conditions with no negative effect of the Bt toxin on decomposition processes.     

Mycorrhizal colonization and nitrogen uptake by maize: combined effect of tropical earthworms and velvetbean mulch

Earthworms and mulch can have positive or negative effects on mycorrhizae (fungus-roots) and N uptake by plants. In the present experiment, maize plants were grown under greenhouse conditions with or without tropical earthworms (Balanteodrilus pearsei) and mulch of velvetbean (Mucuna pruriens var. utilis). The formation of vesicles and hyphae of arbuscular-mycorrhizal (AM) fungi in roots and N uptake by maize plants was measured at harvest. The addition of earthworms and velvetbean reduced AM root colonization. Earthworms had no effect on plant root or shoot biomass. In the absence of velvetbean, earthworms reduced AM colonization, but when velvetbean was present, this effect disappeared. The addition of velvetbean mulch, on the other hand, had an effect on plant biomass (above- and belowground) and a positive effect on AM fungal colonization of roots in presence of worms, but a negative effect when worms were absent. When both M. pruriens and B. pearsei were added, shoot and root biomass and N concentrations increased. Vesicle formation was related to velvetbean mulch decomposition as well as the higher N concentration in maize roots. Management of mulch–earthworm interactions may be of value, particularly in low-input and organic agricultural systems, and deserves further investigation.     

Earthworm populations and growth rates related to long-term crop residue and tillage management

Conventional tillage creates soil physical conditions that may restrict earthworm movement and accelerate crop residue decomposition, thus reducing the food supply for earthworms. These negative impacts may be alleviated by retaining crop residues in agroecosystems. The objective of this study was to determine the effects of various tillage and crop residue management practices on earthworm populations in the field and earthworm growth under controlled conditions. Population assessments were conducted at two long-term (15+ years) experimental sites in Québec, Canada with three tillage systems: moldboard plow/disk harrow (CT), chisel plow or disk harrow (RT) and no tillage (NT), as well as two levels of crop residue inputs (high and low). Earthworm growth was assessed in intact soil cores from both sites. In the field, earthworm populations and biomass were greater with long-term NT than CT and RT practices, but not affected by crop residue management. Laboratory growth rates of Aporrectodea turgida (Eisen) in intact soil cores were affected by tillage and residue inputs, and were positively correlated with the soil organic C pool, suggesting that tillage and residue management practices that increase the soil organic C pool provide more organic substrates for earthworm growth. The highest earthworm growth rates were in soils from RT plots with high residue input, which differed from the response of earthworm populations to tillage and residue management treatments in the field. Our results suggest that tillage-induced disturbance probably has a greater impact than food availability on earthworm populations in cool, humid agroecosystems.     

The Maximum Incremental Social Tolerable Irreversible Costs (MISTICs) and other benefits and costs of introducing transgenic maize in the EU-15

The decision to release a new transgenic crop variety for planting in the European Union (EU) is a decision under irreversibility and uncertainty. We use a real option model to assess the ex-ante incremental benefits and costs of the decision to release Bt maize and HT maize in the EU-15 member states. The analysis uses Eurostat data for modelling the benefits and costs of non-transgenic maize using partial equilibrium models. The farm-level benefits and costs of Bt maize and HT maize are derived from field trials conducted within the EU-funded ECOGEN project in combination with secondary data sources. Adoption curves, hurdle rates and Maximum Incremental Social Tolerable Irreversible Costs (MISTICs) are calculated at country level for selected EU-15 member states. In general, the results show that the MISTICs on a per capita level are very small confirming previous results calculated in values for the year 1995. The MISTICs per farm are much larger. This indicates a problem for decision makers.     

不同轮耕方式对渭北旱塬麦玉轮作田土壤物理性状与产量的影响

为研究不同轮作模式对渭北旱作冬小麦?春玉米一年1熟轮作田土壤物理性状和产量的影响,于2007—2014年在陕西省合阳县冬小麦?春玉米轮作田连续7年实施了保护性耕作定位试验,测定和分析了免耕/深松、深松/翻耕、翻耕/免耕、连续免耕、连续深松和连续翻耕6种轮耕模式下麦田0~60 cm土层物理性状、0~200 cm土层土壤湿度和小麦产量的变化。结果表明:1)不同轮耕模式0~40 cm土层土壤容重、孔隙度和田间持水量差异显著,其中以免耕/深松效果最显著;0~60 cm土层免耕/深松轮耕处理平均田间持水量较连续翻耕处理提高12.9%;2)轮耕对土壤团聚体特性影响明显,免耕/深松0.25 mm水稳性团聚体含量(R0.25)最高,结构体破碎率和不稳定团粒指数(ELT)最低,水稳性均重直径(WMWD)最高,水稳性和力稳性团聚体分形维数(D)均最低;3)小麦生育期间免耕/深松处理0~200 cm土层土壤蓄水量和小麦产量较连续翻耕分别增加17.7 mm和9.5%。综合可知,轮耕有利于耕层土壤物理结构改善,免耕/深松更有利于耕层土壤大团聚体形成和土壤结构稳定,利于土壤蓄水保墒和作物增产,为渭北旱塬区麦玉轮作田较适宜的轮耕模式。     

Effects of Bt-maize material on the life cycle of the land snail Cantareus aspersus

Insect resistant Bt-maize (MON 810) expresses active Cry1Ab endotoxin derived from Bacillus thuringiensis (Bt). Snails constitute non-target soil species potentially exposed to Bt-toxin through consumption of plant material and soil in fields where transgenic plants have been grown. We studied the effect of the Cry1Ab toxin on survival, growth and egg hatchability of the snail Cantareus aspersus. From the age of 4 to 88 weeks, snails were fed either powdered Bt-maize or non-Bt-maize and exposed to soil samples collected after harvesting either the Bt-maize or non-Bt-maize. We applied four treatments: non-Bt soil + non-Bt-maize (MM); Bt soil + Bt-maize (BB), non-Bt soil + Bt-maize (MB), Bt soil + non-Bt-maize (BM). Eggs laid by snails not exposed to Bt-toxin were also exposed to the two types of soils (Bt and non-Bt soil).At the end of growth (47 weeks of exposure), snails exposed to Bt-toxin in food and soil (BB) had a growth coefficient (GC) 25% lower than unexposed snails (MM). After the first period of reproduction (68 weeks) a significant difference remained for body mass GC between the BB and MM treatments. Differences in body mass were not significant at the end of exposure (88 weeks). For snails not previously exposed to Bt material, hatchability of eggs was similar in the soils tested. The outcome of the experiments indicates that, in growing snails, long-term exposure is needed to reveal an effect of Bt-maize. The hazard analysis of Bt-maize which we performed, based on a worst-case scenario, i.e. snails having no food choice, should now be complemented by other simple measurements, e.g. food intake, to understand the underlying mechanisms involved.     

Influence of reduced tillage on earthworm and microbial communities under organic arable farming

Although reduced tillage is an agricultural practice reported to decrease soil erosion and external inputs while enhancing soil fertility, it has still rarely been adopted by European organic farmers. The objective of this study was to assess the long-term interactive effects of tillage (conventional (CT) vs. reduced (RT)) and fertilization (slurry (S) vs. composted manure/slurry (MCS)) on earthworms and microbial communities in a clay soil under spelt in an organic 6-year crop rotation. Earthworm populations (species, density and biomass, cocoons) were investigated by handsorting the soil nine years after initial implementation of the treatments. Soil microbial carbon (C_mic) and nitrogen (N_mic) were measured by chloroform-fumigation extraction and a simplified phospholipid fatty acid (PLFA) analysis was used to separate for populations of bacteria, fungi and protozoa. Significantly increased total earthworm density in RT plots was mainly attributed to increased numbers of juveniles. Moreover, we found five times more cocoons with RT. Species richness was not affected by the treatments, but tillage treatments had differentially affected populations at the species-level. In addition, cluster analysis at the community level revealed two distinct groups of plots in relation to tillage treatments. In RT plots C_mic increased in the 0–10 cm and 10–20 cm soil layers, while PLFA concentrations indicative of Gram-negative bacteria, fungi and protozoa only increased in the topsoil. Lower bacteria-to-fungi ratios in the upper soil layer of RT plots indicated a shift to fungal-based decomposition of organic matter whereas a higher C_mic-to-C_org ratio pointed towards enhanced substrate availability. Slurry application decreased microbial biomass and enhanced density of juvenile anecic earthworms but overall fertilization effect was weak and no interactions with tillage were found. In conclusion, tillage is a major driver in altering communities of earthworms and microorganisms in arable soils. The use of reduced tillage provides an approach for eco-intensification by enhancing inherent soil biota functions under organic arable farming.     

Reduced tillage in temperate organic farming: implications for crop management and forage production

To promote conservation tillage in organic farming systems, weed control and ley removal within arable-ley rotations need to be optimized. A long-term field trial was thus established in Frick, Switzerland in 2002 on a clayey soil and with a mean precipitation of 1000 mm/year. The tillage experiment distinguished between conventional tillage with mouldboard ploughing (CT, 15 cm depth) and reduced tillage (RT), including a chisel plough (15 cm) and a stubble cleaner (5 cm). Results of a 2-year grass-clover ley (2006/2007) and silage maize (2008) are presented. Due to dry conditions, mean grass-clover yields were 25% higher in RT than in CT, indicating better water retention of RT soils. Clover cover and mineral contents of the fodder mixture were also higher in RT. The ley was successfully removed in autumn 2007 in RT plots, and a winter pea catch crop was sown before maize. In CT, ploughing took place in spring 2008. Maize yields were 34% higher in RT than in CT, despite a two- to three-fold higher but still tolerable weed infestation. Maize in RT plots benefited from an additional 61.5 kg of easily decomposable organic N/ha incorporated into the soil via the pea mulch. Measurement of arbuscular mycorrhizal colonization of maize roots indicated a similar mechanical disturbance of the topsoil through the reduced ley removal system compared with ploughing. It is suggested that RT is applicable in organic farming, even in arable-ley rotations, but long-term effects need further assessment.     

秸秆还田与旋耕对川中土壤物理性状及玉米机播质量的影响

为明确川中丘陵地区紫土小麦-玉米两熟种植模式下机播玉米生产适宜的秸秆还田方式与耕作方式,改善土壤的瘠薄、黏性过大等问题,提高耕层土壤的播种条件、播种质量,达到作物高产的目的,设置秸秆还田旋耕(RTS)、秸秆还田免耕(NTS)、秸秆不还田旋耕(RT)、秸秆不还田免耕(NT)4个处理,研究麦秸还田与旋耕对川中丘区紫色黏土物理性状及机播夏玉米播种质量的影响。结果表明:与秸秆不还田相比,麦秸还田显著增加了拔节期0~10 cm土层土壤毛管孔隙度和免耕处理0~10 cm全生育时期土壤含水量,显著降低出苗率、播种均匀度和幼苗整齐度等指标。与免耕相比,旋耕处理降低了0~10 cm土壤容重、含水量,增加了毛管孔隙度,出苗率提高9.9%,幼苗株高、茎粗、叶面积和干重显著提高。麦秸还田条件下,旋耕处理较免耕处理0~10cm土壤容重降低2.0%~12.1%,出苗率、播种均匀度、每穴苗数、幼苗整齐度显著提高,其中出苗率增加17.9%。玉米出苗率与0~10 cm土壤含水量呈显著正相关,播种均匀度与幼苗整齐度均与0~10 cm土壤容重呈显著负相关。可见,麦秸还田下旋耕处理改善了土壤结构,增加了土壤含水量,更有利于川中丘陵地区小麦-夏玉米种植模式机播玉米质量和幼苗素质的提高。     

华北平原缺水区保护性耕作技术

针对华北平原缺水地区农田生产效益偏低和地下水严重超采导致的生态环境问题,以建立节水、高产、固碳的华北平原缺水区保护性耕作集成技术为目标,在国家科技支撑计划长期支持下,建立了华北平原历时最长的保护性耕作长期定位试验平台(2001年—),开展了小麦/玉米两熟制保护性耕作理论和关键技术研究,集成了农机农艺结合的高产节水型保护性耕作技术体系,并在河北省进行广泛示范推广。主要结果:1)华北平原冬小麦/夏玉米一年两熟区保护性耕作具有固碳、减排、节水、提高土壤质量等生态效应。长期保护性耕作具有土壤养分分层表聚现象:0~5 cm土层的土壤C、N、P、K、有机质含量高于5~10 cm土层,旋耕(RT)和免耕(NT1:秸秆直立免耕;NT2:秸秆粉碎免耕;NT3:整秸秆覆盖免耕)处理土壤有机碳(SOC)的层化比率为1.74~2.04,显著高于翻耕处理(CK和CT)的1.37~1.45。保护性耕作的固碳效应与机制:保护性耕作实施9年后不同耕作方式年固碳量(0~30 cm)NT2处理为840 kg·hm~(-2)·a~(-1)、RT处理为780 kg·hm~(-2)·a~(-1)、CT处理为600kg·hm~(-2)·a~(-1),14年后土壤有机碳(0~30 cm)发生了变化,NT2处理为540 kg·hm~(-2)·a~(-1)、RT处理为720 kg·hm~(-2)·a~(-1)、CT处理为710 kg·hm~(-2)·a~(-1);长期免耕减少了土壤的扰动而降低了土壤碳的矿化率,土壤碳的累积主要固定在土壤大团聚体的颗粒有机碳中,固定态碳首先进入活性易分解有机碳库,然后缓慢转入稳定碳库。保护性耕作的减排效应:不同耕作系统全球增温潜力的计算结果表明,免耕是大气增温的碳汇,而其他耕作系统为碳源。NT处理每年农田生态系统净截留碳947~1 070 kg(C)·hm-2;CK、CT和RT每年向大气分别排放等当量碳3 364kg(C)·hm-2、989 kg(C)·hm-2和343 kg(C)·hm-2。保护性耕作的土壤微生物多样性机制:保护性耕作显著提高了土壤中真菌、细菌、氨氧化古菌和亚硝酸还原酶(nir K)基因的反硝化微生物的多样性,但对氨氧化细菌与含nir S基因的反硝化微生物的多样性影响不大。保护性耕作节水保墒的土壤结构与水力学机制:常规耕作对土壤有压实的作用,而保护性耕作改善了土壤结构,有效提高了储水孔隙、导水率、田间持水量和有效水含量,秸秆覆盖又能有效减少土壤蒸发,具有开源与节流双重节水机制。2)建立了趋零蒸发的麦田玉米整秸覆盖全免耕种植模式。在小麦/玉米一年两熟种植区,首次提出了玉米整秸秆覆盖小麦全免耕播种的种植模式,实现了小麦玉米全程全量秸秆机械化覆盖,形成土壤无效蒸发趋于零的保护性耕作体系与方法;研制了实现趋零蒸发的4JS-2型梳压机和2BMF-6型小麦全免耕播种机组,比目前推广的2BMFS-6/12小麦免耕播种机减少作业动力45.2%,降低作业费用33.3%。3)建立了3年一深松(翻)的少免耕-深松轮耕模式,集成了节水高产保护性耕作技术体系。制定了华北平原冬小麦/夏玉米一年两熟区保护性耕作技术体系等河北省地方标准,与农业、农机部门联合示范,推动了河北省保护性耕作技术的推广和应用。成果在河北平原冬小麦/夏玉米一年两熟区进行了示范推广,社会效益和生态效益显著,2013年获河北省科技进步一等奖。