Reduced inter-row distance improves yield and competition against weeds in a semi-dwarf durum wheat variety

Weed and nutrient management in cropping systems of semi-arid areas is a major constraint to cereal yield. Where the use of herbicides is banned or discouraged, the competitive ability of a crop is crucial to reduce weed growth and diffusion. Genotypic differences in the competitive abilities of crops are an important trait to reduce weeds, especially for plant height. However, there is contrasting information about the interactions of other management practices and genotypic traits on wheat yield and competitive ability against weeds and weed growth. The present study investigated yield and quality of durum wheat (Triticum durum Desf.) and weed growth and composition for two wheat cultivars with contrasting competitive abilities against weeds. Wheat was grown under three spatial arrangements (5-cm, 15-cm, 25-cm inter-row distance) and three sowing densities, and broadleaf weeds were either removed or not. The sowing rate did not affect the yield of these wheat cultivars or the weed growth. Reduced inter-row distance dramatically reduced weed biomass for both wheat cultivars, and increased wheat yield and nitrogen uptake in the low-competitive, high-yielding, semi-dwarf cv. ‘PR22D89’, when both weed free and with weeds. These results have direct implications for weed and nutrient management in low-input and organic cropping systems.     

Intergenotypic competition in mixed stands of spring wheat genotypes

Summary Grain yield and yield components were studied in the three spring wheat genotypes HD 2160 (dwarf), Kalyansona (semi-dwarf) and C 306 (tall) in pure stands as well as in their binary mixed stands during two years. The grain yields of the three genotypes in pure stands ranked as follows: HD 2160 > Kalyansona > C 306. Four mixed stands, 3:1 HD:K, 1:1 HD:C in the first year and 1:1 K:C, 1:3 K:C in the second year out-yielded the pure stand of the better component genotype by 4.4, 2.7, 3.3 and 0.8 percent, respectively. Out of the nine mixed stands four in the first year and seven in the second year out-yielded the midmonoculture yields and the increases ranged from 1 to 7.6 percent. Mixed stands were more stable than pure stands.The yield and yield components of the dwarf genotype HD 2160 scored less and those of the taller genotype C 306 scored higher in mixed stands. The semi-dwarf genotype Kalyansona yielded more with HD 2160 and less with C 306. Plant height but not high yielding ability conferred high competitive ability. With respect to competitive ability the three genotypes ranked as follows: C 306 > Kalyansona > HD 2160.The results illustrate the importance of intergenotypic competition in increasing crop production and reducing genotype-environment interactions. Such studies are important to agronomists as genotypes with high competitive ability can be useful to combat the weed problem. They are also important to plant breeders for predicting the fate of genotypes with low competitive ability in heterogeneous populations.     

Effect of spring fertilization on ecosystem services of organic wheat and clover relay intercrops

Nitrogen (N) deficiency and weed infestation are main factors limiting yield and yield stability in organic wheat. Organic fertilizers may be used to improve crop performance but off-farm input costs tend to limit profitability. Instead, forage legumes may be inserted into the crop rotation to improve the N balance and to control weed infestation. In opposition to simultaneous cropping, relay intercropping of legumes in organic winter wheat limits resource competition for the legume cover crop, without decreasing the performance of the associated wheat.The aim of this study is to evaluate the effect of spring organic fertilization on the performance of intercropped legumes and wheat, and on services provided by the legume cover.Two species of forage legumes (Trifolium pratense L. and Trifolium repens L.) were undersown in winter wheat (Triticum aestivum L. cv Lona) in five organic fields during two consecutive crop seasons. Organic fertilizer was composed of feather meal and applied on wheat at legume sowing. The cover crop was maintained after the wheat harvest and destroyed just before sowing maize.Spring organic nitrogen fertilization increased wheat biomass (+35%), nitrogen (+49%), grain yield (+40%) and protein content (+7%) whatever the intercropping treatment. At wheat harvest, red clover biomass was significantly higher than white clover one (1.4 vs. 0.7 t ha⁻¹). Nitrogen fertilization decreased forage legume above-ground biomass at wheat harvest, at approximately 0.5 t ha⁻¹ whatever the specie. No significant difference in forage legume biomass production was observed at cover killing. Nitrogen accumulation in legume above-ground tissues was significantly higher for white clover than for red clover. Both red and white clover species significantly decreased weed infestation at this date. Nitrogen fertilization significantly increased weed biomass whatever the intercropping treatment and decreased nitrogen accumulation in both clover species (−12%).We demonstrated that nitrogen fertilization increased yield of wheat intercropped with forage legume while the performance of legumes was decreased. Legume growth was modified by spring fertilization whatever the species.     

Environmental and genetic determination of protein content and grain yield in durum wheat under Mediterranean conditions

The unpredictability of the Mediterranean climate causes fluctuations in wheat yield and quality, but offers the opportunity for obtaining high‐quality durum wheat in terms of grain protein content. Twenty‐five durum wheat genotypes were grown under irrigated and rainfed conditions at each of two latitudes in Spain during 1998 and 1999. Differences between latitudes in grain protein content and chlorophyll content in the flag leaf were attributable to nitrogen fertilization management. Cycle length until anthesis was less affected by the environment than grain‐filling duration, and was longer under irrigated conditions than in the rainfed sites. A negative asymptotic curve was the best equation to fit the relationship between yield and protein content, suggesting that yield improvements in fertile environments may be attained with negligible reductions in protein content. ‘Jabato’, ‘Waha’, ‘Lagost‐3’, ‘Massara‐1’ and ‘Vit?on’ showed medium to high yield, yield stability and high protein content. Chlorophyll content in the flag leaf, measured at anthesis with the soil‐plant analysis development (SPAD) portable field unit, may be useful for the fast and cheap detection of durum wheat genotypes with high grain protein content in drought‐stressed Mediterranean environments.     

Growth and Development of Six Barley (Hordeum vulgare ssp. vulgare L.) Cultivars in Response to a Model Weed (Sinapis alba L.)

The aim was to study the growth and development of six spring barley (Hordeum vulgare ssp. vulgare L.) cultivars as a response to a model weed population of Sinapis alba L. The development of light interception profiles over time was characterized for each cultivar in weed‐free stands. The cultivars were chosen such that they represent a range of weed‐suppressive abilities based on previously performed trials. One field experiment each was conducted in 1996 and 1997 at a site SE of Uppsala, Sweden. The two cultivars with low competitive ability against weeds, Etna and Blenheim, allowed the highest weed biomass and the lowest crop biomass in competition with the weeds. These two cultivars transmitted more photosynthetic active radiation through the canopy down to 20 and 40 cm height than did the other cultivars. Although the biomass of cv. Etna was low, the grain yield was higher than that of the other cultivars when grown in competition with weeds. In 1997, cv. Svani with good competitive ability against weeds transmitted least light and had greater grain yield than most other cultivars. The absence of a relationship between high grain yield and low weed suppressive ability in the present study indicates that it should be possible for plant breeders to combine high grain yielding capacity with approved weed‐competitive ability.     

Weed tolerance in soybean: a direct selection system

Weed competition can severely reduce soybean (Glycine max (L.) Merr.) yields, particularly in organic systems. An efficient screening and breeding approach is needed to increase breeding progress for weed tolerance. This study sought to (i) establish a system for direct selection of competitive genotypes, (ii) evaluate genotypic differences in weed tolerance among six early‐maturing genotypes and (iii) assess the contribution of selected morphological traits to weed tolerance. A direct selection system providing two different levels of weed competition through all development stages of a soybean crop was developed, using mixtures of selected crop species as sown competitors. Two resulting mixtures induced intermediate (<30%) and strong (>50%) yield reduction, respectively. This selection system can be applied in screening and breeding programmes to facilitate breeding for weed tolerance. No significant difference in weed tolerance was detected between six soybean genotypes of maturity groups 000 to 00. Morphological traits that might influence competitive ability, for example light absorption, leaf area and lateral shoots, were assessed, and their potential for indirect selection for weed tolerance is discussed.     

Genotypic and phenotypic evaluation of wild cotton accessions previously identified as resistant to root-knot (Meloidogyne incognita) or reniform nematode (Rotylenchulus reniformis)

Although the effect of local adaptation is well documented in evolutionary biology, few studies have quantified the impact of local adaptation in plant breeding. Decentralized plant breeding programs have the potential to harness local adaptation for crop improvement, but the effectiveness of such models is understudied. We quantified the ability of a decentralized participatory plant breeding program to improve Weed-competitive ability (WCA) in organic spring wheat. After four farmers in the northeast United States selected wheat populations for WCA and its correlated trait of early vigor, we tracked gains in selection and local adaptation. On average, farmers enhanced competitive ability of selected genotypes by 11.46%. Measured gains from selection for early vigor and early canopy cover, however, varied among testing environments. Gains in selection were highly related to the genetic correlation coefficient between selection and testing environment (r?=?0.77 and r?=?0.80 for early vigor and canopy cover, respectively). To accurately measure gains from selection for decentralized breeding programs, testing environments should be chosen that are similar to where selection took place. Inconsistent weed competition among site-years limited conclusions from the analysis of local adaptation for weed competitive ability. Detecting local adaptation in plant breeding, which typically uses a small number of selection cycles compared to evolutionary biology, likely requires many genotypes, environments, and years for adequate statistical power. The ecological complexity of weed competitive ability further complicates experimental design and challenges the ability to measure local adaption.

     

Crop and Weed Growth in a Sequence of Spring Barley and Winter Wheat Crops Established Together from a Spring Sowing (Relay Cropping)

A relay cropping system of cereals, whereby winter wheat (Triticum aestivum L.) was undersown in two‐row spring barley (Hordeum distichum L.), was established in a field trial in central Sweden in 1999 and continued until 2000. The purpose of the study was to examine crop and weed responses to different plant densities of the undersown winter crop. Winter wheat was sown at four seed rates (187, 94, 47 and 0 kg ha^?1) immediately after the sowing of barley. Barley was harvested in the first autumn after sowing and winter wheat in the second autumn. The grain yield of barley was not affected by the seed rate of wheat, and averaged 4580 kg ha^?1. Winter wheat did not vernalize during the first growing season but remained at the vegetative stage. The grain yield of wheat was 1990 kg ha^?1 for the lowest and 5610 kg ha^?1 for the highest seed rate of wheat. Whilst the undersowing process itself stimulated weed emergence in this experiment, increasing the undersowing seed rate reduced the population of perennial weeds by 40–70 %. In the second growing season, the total biomass of weeds was 66 % higher at the highest seed rate compared with the lowest seed rate.     

Cover crops and interrow tillage for weed control in short season maize (Zea mays)

Weed competition can cause substantial maize (Zea mays L.) yield reductions. Interseeding maize with cover crops or a combination of interrow cultivation and interseeded cover crops are possible alternative methods of weed control. This study was conducted to examine the potential of interrow cultivation plus cover crops to reduce weed density in maize without reducing the grain yield. Field experiments were conducted in 1993 and 1994 at two sites in Québec to determine the effects of planting 12 cover crops with maize on weed control. Fall rye (Secale cereal L.), hairy vetch (Vicia villosa Roth), a mixture of red clover (Trifolium pratense L.) and ryegrass (Lolium multiflorum Lam), a mixture of white clover (Trifolium repens L.) and ryegrass, subterranean clover (Trifolium subterraneum L.), yellow sweet clover (Meliotus officinalis Lam), black medic (Medicago lupulina L.), Persian clover (Trifolium resupinatum L.), strawberry clover (Trifolium fragiferum L.), crimson clover (Trifolium incarnatum L.), alfalfa (Medicago sativa L.), and berseem clover (Trifolium alexandrinum L.) were seeded at two planting dates, 10 and 20 days after maize emergence. Interrow cultivation was carried out weekly until forage seeding, with a final cultivation being conducted just prior to cover crop seeding. Cover crop planting date did not affect maize yields or the ability of interrow tillage plus cover crops to suppress the development of weed populations. Maize yield was less affected by the interseeded cover crops under conditions of adequate rainfall. Corn planted in fields heavily infested with weeds resulted in substantial yield reductions even when rainfall was adequate. Except for 1993 at l'Assomption interrow tillage plus cover crop treatments had consistently lower weed biomass when compared to the weedy control. Most of the weed control was due to the interrow cultivation performed prior to seeding of the cover crops. The lowest weed density occurred in the herbicide treated plots. The ability of interrow tillage plus cover crops to suppress the development of weeds was affected by the level of weed infestation, the growing conditions and location. The cover crops provide additional weed control but the interrrow tillage or some herbicide application may still be necessary.     

Legume cover crops as living mulches for winter wheat: Components of biomass and the control of weeds

To gain information about the possible use of legume cover crops as an alternative and sustainable weed-control strategy for winter wheat (Triticum aestivum L.), an experiment was conducted at two sites in the Swiss Midlands in 2001/2002. Under organic farming conditions winter wheat was direct-drilled into living mulches established with four different legume genotypes or into control plots without cover crops. Compared to NAT (control plots without cover crops but with a naturally establishing weed community), white clover (Trifolium repens L.), subclover (Trifolium subterraneum L.), and birdsfoot trefoil (Lotus corniculatus L.) reduced the density of monocotyledonous, dicotyledonous, spring-germinating, and annual weeds by the time of wheat anthesis. Strong-spined medick (Medicago truncatula Gaertner) was less efficient in this regard. While the grain yield was reduced by 60% or more for all legumes when compared to NOWEED (control plots kept weed-free), a significant negative correlation between the dry matter of the cover crop and weeds as well as between the cover crop and the winter wheat was observed by the time of wheat anthesis. The effect of manuring (60 m³ ha⁻¹ liquid farmyard manure) was marginal for weeds and cover crops but the additional nutrients significantly increased total winter wheat dry matter and grain yields. The suppression achieved by some legumes clearly demonstrates their potential for the control of weeds in such cropping systems. However, before living legume cover crops can be considered a viable alternative for integrated weed management under organic farming conditions, management strategies need to be identified which maximise the positive effect in terms of weed control at the same time as they minimise the negative impact on growth and yield of winter wheat.     

Competition and Control of Volunteer Jerusalem Artichoke in Various Crops

A major production constraint in Jerusalem artichoke ( Helianthus tuberosus L.) is caused by tubers which are not recovered at harvest. Such lost tubers raise a serious weed problem the following season. Winter wheat, oat, spring oilseed rape, sugarbeet, maize and ryegrass were grown in a field which had Jerusalem artichoke as the preceding crop in order to obtain information about their competitive ability and the efficacy of various control measurements against Jerusalem artichoke infestation. The Jerusalem artichoke treatments in these crops were: total control by regular hand weeding (TOC), mechanical/chemical control (MCC), and no control (NOC). Under the NOC treatment, Jerusalem artichoke infestation at harvest was variable among crops, with the number of shoots ranging from 9 to 25 m⁻² in oat and maize stands respectively. The number of Jerusalem artichoke shoots in the MCC plots was reduced by 50 to 99 % in oat and maize, respectively. The highest crop yields in each of the six species were realized under the TOC treatment. Insignificant yield reductions were observed in the NOC treatment of wheat, oat, rape and ryegrass. However, under this management yield reductions of 91 and 81 % occurred in sugarbeet and maize respectively. Depending on the preceding crop, 1–9 shoots m⁻² of Jerusalem artichoke were still recorded under the MCC plots in the following season. Consequently, for complete elimination of infestation, volunteers must be controlled in the second and probably in the third year following a Jerusalem artichoke crop.     

Relay-intercropped forage legumes help to control weeds in organic grain production

In organic grain production, weeds are one of the major limiting factors along with crop nitrogen deficiency. Relay intercropping of forage legume cover crops in an established winter cereal crop might be a viable option but is still not well documented, especially under organic conditions.Four species of forage legumes (Medicago lupulina, Medicago sativa, Trifolium pratense and Trifolium repens) were undersown in six organic wheat fields. The density and aerial dry matter of wheat, relay-intercropped legumes and weeds were monitored during wheat-legume relay intercropping and after wheat harvest until late autumn, before the ploughing of cover crops.Our results showed a large diversity of aerial growth of weeds depending on soil, climate and wheat development. The dynamics of the legume cover crops were highly different between species and cropping periods (during relay intercropping and after wheat harvest). For instance, T. repens was two times less developed than the other species during relay intercropping while obtaining the highest aerial dry matter in late autumn. During the relay intercropping period, forage legume cover crops were only efficient in controlling weed density in comparison with wheat sole crop. The control of the aerial dry matter of weeds at the end of the relay intercropping period was better explained considering both legumes and wheat biomasses instead of legumes alone. In late autumn, 24 weeks after wheat harvest, weed biomass was largely reduced by the cover crops. Weed density and biomass reductions were correlated with cover crop biomass at wheat harvest and in late autumn. The presence of a cover crop also exhibited another positive effect by decreasing the density of spring-germinating annual weeds during the relay intercropping period.     

Dynamisches Entscheidungsmodell zur Lenkung von Unkrautkontrollmaßnahmen in Wintergetreide mit Hilfe digitaler Bildverarbeitung

Dynamic decision model for weed control methods in cereals by means of digital image analysis
Field experiments were conducted to study weed-crop competition in winter wheat, -barley and -rye. Weed cover and crop cover was determined over time by digital image analysis of slides taken in the field. The relation of percentage weed coverage and crop coverage was most suitable to quantify the competition of weed population on the crop. In addition the model "UNPROG" was established to predict yield losses caused by weed competition and to estimate the profitability of chemical or mechanical weed control. The model was tested on 22 farms in Nordrhein-Westfalia in autumn 1991 and spring 1992. In 65 out of 72 field plots (92 %) of winter cereals "UNPROG" gave correct prediction with respect to necessity of weed control.     

Investigations on Heat Tolerance of Spring Wheat Varieties of Different Origin Under Growth Chamber Conditions

Winter wheat has replaced spring wheat to a large extent under the climatic conditions of Western and Central Europe. But spring wheat genotypes are still important under the warm climate in large parts of Africa and Central America, since spring wheat needs no vernalization. To reach optimal yield, genotypes must be well adapted to heat stress. In this study 11 spring wheat varieties of Egypt, Sudan, CIMMYT and Germany were exposed to high temperatures at ear emergence and beginning of anthesis. There were reductions in kernel yield and kernel number of the main ear for all genotypes, but the differences in reduction were remarkable. The German variety 'Naxos' was best adapted. Some varieties showed an increased number of florets per spikelet under heat treatment, but this does not prohibit a reduction in kernel number per spikelet.     

Crop sequence,crop protection and fertility management effects on weed cover in an organic/conventional farm management trial

A survey of 128 plots, in 2008, of a trial where the effects of crop protection can be separated from those of fertility management, generated weed cover data within six crops (winter wheat, winter barley, spring barley, potatoes, cabbages and a grass/clover ley). The effects of the 2008 crop types, of the two preceding crops and of organic and conventional crop protection and fertility management, were assessed using mixed-effects models and constrained ordination. Cover data for 22 weed species and for monocotyledon, dicotyledon, annual, perennial and total weed cover were used. Cover of 15 weed species, and of the five weed groups, was significantly affected by 2008 crops, with cover highest in spring beans and cabbage. Nine and four weed species 2008 cover were significantly related to crops grown in 2007 and 2006 respectively, as were dicotyledon, annual and total weed cover, but not monocotyledon or perennial cover. Cover of 15 species, and the five groups, was significantly higher in plots with organic crop protection, but only eight species and annuals were significantly affected by fertility management. Crop:crop protection produced the most significant interactions with most cover in organically managed plots. Five species, perennials and total weed cover produced significant three-factor models. The greatest weed cover was in organic crop protected but conventionally fertilised spring barley and the least in totally conventional winter barley. Other factors such as crop density and mechanical weeding also affected 2008 weed cover. The ordination indicated that most of the 22 species were strongly associated with crops from all three years. The sequence of crops in the rotation had a profound effect on weed cover. Where three spring-sown, difficult to weed, crops were grown in sequence (spring beans, potatoes and vegetables, spring barley) weed cover increased. However, cover was limited in grass/clover and some cereal plots with different preceding crops. Models predicting weed cover may need to take into account crop sequences within crop rotations, as well as the more usual management inputs.     

Abortion of infection structures of wheat leaf rust in susceptible and partially resistant wheat genotypes

Summary Arrest of the growth of wheat leaf rust infection structures was studied with fluorescence microscopy in seedling leaves and flag leaves of the susceptible spring wheat genotypes Morocco and Kaspar and the partially resistant genotypes Westphal 12A and Akabozu. The percentages non-penetrants and substomatal vesicle abortion were low in all genotypes. In the partially resistant genotypes the percentage abortion of infection structures was higher than in the susceptible genotype Morocco. Aborted infection structures had formed one or two haustorial mother cells. In adult plants differences in the percentage aborted infection structures between susceptible and partially resistant genotypes were more pronounced than in seedlings. The so-called late abortion was not observed.     

Analysis of genotypic variation for normalized difference vegetation index and its relationship with grain yield in winter wheat under terminal heat stress

Normalized difference vegetation index (NDVI), which is a measure of leaf greenness (chlorophyll content), is considered to be correlated with crop productivity. This study was conducted to examine genotypic variations for NDVI at different growth stages and its relationship to yield in winter wheat under terminal heat stress. Thirty winter wheat genotypes were evaluated at two locations in 2009–2010 and 2010–2011 in Uzbekistan. The NDVI was recorded at booting, heading, milk and dough stages. The wheat genotypes differed significantly for NDVI at each stage. Grain yield ranged from 3.9 to 6.1 t/ha. Wheat genotypes differed in per cent decline in NDVI from booting to dough stage. However, several high‐yielding genotypes maintained higher NDVI than low‐yielding genotypes when heat stress was evident. The findings suggest change in NDVI during heat stress could be a measure of tolerance. The positive correlation of NDVI with grain yield suggests that it could be used as an indirect selection criterion for identifying physiologically superior, high‐yielding wheat lines under terminal heat stress.     

基因型和灌溉对小麦旗叶保卫细胞长度的影响

观察来源于16个小麦基因型在有、无灌溉条件下,旗叶保卫细胞长度的变化.研究结果表明,基因型、灌溉及基因型和灌溉互作与旗叶保卫细胞的长度及其分布有密切关系.在灌溉条件下,旗叶保卫细胞相对较短,分布范围较集中,在旱地中,旗叶保卫细胞相对较长,分布范围也较集中.在水地和旱地中,小麦旗叶保卫细胞长度分布相同者一般为耐旱性较强的品种.反之,则为耐旱性较差的品种.     

Clover as a cover crop for weed suppression in an intercropping design: II. Competitive ability of several clover species

Undersown cover crop species introduced for weed management purposes should ideally combine adequate weed suppression with only marginal negative competitive effects on the main crop. The aim of this research was to identify the growth characteristics of clover species that determine weed suppressive ability and competitive ability against the main crop. In addition, the variation in these characteristics among clover species was determined, to identify whether species selection is an important component of the optimization of living mulch systems.In 2001, a field experiment was conducted in which weed suppression and competitive ability against leek of eight clover types was determined. Based on the results, three contrasting clover types were selected and their weed suppressive ability was further investigated in a field experiment conducted in 2002. Results of both experiments were related to growth characteristics that were simultaneously determined and described in a previous paper.Correlation analysis showed that the competitive effect of clover on transplanted leek was strongly correlated with maximum canopy height, indicating that yield reduction was mainly caused by competition for light. Subterranean clover, being the shortest species, gave inadequate weed suppression, whereas all other species were found to provide at least reasonable weed suppression. Subterranean clover reduced individual leek plant dry weight more strongly (60%) than expected based on its canopy height. Indications were found that this clover species was hindering the uptake of nitrogen by leek. Clover species with superior weed suppression, like Persian clover, red clover, alsike clover, berseem clover and crimson clover also gave the strongest negative effect on dry matter accumulation of leek (reductions between 70% and 90%). White clover was found to give the best compromise between adequate weed suppression and mild yield reduction, though also with this species reduction in leek plant dry weight was still 60%. It was concluded that for optimization of systems that introduce clover species as a weed suppressing cover crop species selection is important element, though additional management to restrict the competitive effect on the main crop remains a requirement.     

Association between Yield and Carbon Isotope Discrimination Value in Different Organs of Durum Wheat Under Drought

Carbon isotope discrimination (Δ) has been proposed as a selection criterion for transpiration efficiency and grain yield in drought‐prone environments for several C³ species, including cereals. Δ analysis, however, has mainly been concerned with grain or culm tissues and little work has been devoted to other organs. The objective of this study was to describe Δ variation in different organs and to examine the relationships between Δ and grain yield across environments. Six durum wheat genotypes with contrasted grain Δ were cultivated under rainfed conditions during three successive years at Montpellier (South of France). Δ was measured on flag leaf, stalk, awns, chaff and rachis sampled at anthesis and maturity, and on mature grain. Higher genotypic variation and closer correlation with yield were noted for grain Δ compared to other plant parts. The genotype ranking across years was more consistent for grain Δ than for other organs. Consequently, the grain seems the most effective plant part for Δ analysis in durum wheat under Mediterranean conditions. The study of Δ variation in other organs may be useful, however, to evaluate the contribution of those organs to grain filling and final yield according to environmental conditions.