Reduced energy density of close‐up diets decrease ruminal pH and increase concentration of volatile fatty acids postpartum in Holstein cows

The objective of this study was to determine the effect of reduced energy density of close‐up diets on ruminal fermentation parameters in transition cows. Fourteen Holstein dry cows were blocked and assigned randomly to three groups fed a high energy density diet (HD, 1.62 Mcal of net energy for lactation (NE_L)/kg dry matter (DM)), or a middle energy density diet (MD, 1.47 Mcal NE_L/kg DM), or a low energy density diet (LD, 1.30 Mcal NE_L/kg DM) prepartum, and were fed the same diet postpartum. The reduced energy density diets decreased the average dry matter intake (DMI) prepartum and tended to increase the DMI postpartum. The ruminal pH of the LD group was significantly higher prepartum and lower during the first week of lactation compared with the other two groups. The reduced energy density diet depressed the average ruminal concentration of propionate and butyrate prepartum, and increased the average concentration of total volatile fatty acids (VFA) postpartum. The LD group had higher populations of Butyrivibrio fibrisolvens and Ruminococcus flavefaciens relative to HD and MD groups on 7 days in milk. In conclusion, the cows fed reduced energy density diet prepartum had higher VFA concentration, but were more susceptible to subacute ruminal acidosis postpartum.     

Plasma mineral and energy metabolite concentrations in dairy cows fed an anionic prepartum diet that did or did not have retained fetal membranes after parturition

OBJECTIVE: To compare plasma total calcium, phosphorus, magnesium, nonesterified fatty acids (NEFA), beta hydroxy butyrate (BHB), and glucose concentrations in parturient dairy cows that were fed an anionic prepartum diet between those with and without retained fetal membranes (RFM) at 24 hours after parturition. ANIMALS: 152 Holstein cows that calved during October through December of 1997 PROCEDURE: All cows were fed an anionic prepartum diet. Blood sample was taken within 6 hours after parturition from randomly selected cows. Thirty-nine cows had a diagnosis of RFM at 24 hours after parturition; 113 were not affected with RFM. At calving, body condition score (BCS; 1 [thin] to 5 [obese]), parity, and calving difficulty score were recorded. Plasma calcium, phosphorus, magnesium, NEFA, BHB, and glucose concentrations were compared between cows with or without RFM. RESULTS: Cows with RFM had significantly lower plasma calcium concentration soon after calving, compared with cows without RFM. Cows with a parity of > or = 3 had significantly lower plasma concentrations of calcium and higher concentrations of magnesium, compared with cows with a parity of 1 or 2. Cows with a BCS of > or = 3.25 at calving had significantly higher plasma concentrations of BHB than cows with a BCS of 2.75 to 3.0. Cows with dystocia had significantly higher plasma concentrations of glucose, compared with cows without dystocia. CONCLUSIONS AND CLINICAL RELEVANCE: In parturient cows fed a prepartum anionic diet, those with RFM have lower plasma calcium concentrations than cows without RFM, although this association does not prove a cause-effect relationship.     

Effect of reduced energy density of close-up diets on dry matter intake,lactation performance and energy balance in multiparous Holstein cows

Energy intake prepartum is critically important to health,milk performance,and profitability of dairy cows.The objective of this study was to determine the effect of reduced energy density of close-up diets on dry matter intake(DMI),lactation performance and energy balance(EB) in multiparous Holstein cows which were housed in a free-stall bam and fed for ad libitum intake.Thirty-nine dry cows were blocked and assigned randomly to three groups fed a high energy density diet[HD,n = 13;6.8 MJ of net energy for lactation(NE_L)/kg;14.0%crude protein(CP)],or a middle energy density diet(MD,n = 13;6.2 MJ NE_L/kg;14.0%CP),or a low energy density diet(LD,n = 13;5.4 MJ NE_L/kg;14.0%CP) from d 21 before expected day of calving.After parturition,all cows were fed the same lactation diet to d 70 in milk(DIM).The DMI and NE_L intake prepartum were decreased by the reduced energy density diets(P 0.05).The LD group consumed 1.3 kg/d(DM) more diet compared with HD group in the last 24 h before calving.The milk yield and the postpartum DMI were increased by the reduced energy density diet prepartum(P 0.05).The changes in BCS and BW prepartum and postpartum were not affected by prepartum diets.HD group had higher milk fat content and lower lactose content compared with LD group during the first 3 wk of lactation(P 0.05).The energy consumption for HD,MD and LD groups were 149.8%,126.2%and 101.1%of their calculated energy requirements prepartum(P 0.05),and 72.7%,73.1%and 75.2%during the first 4 wk postpartum,respectively.In conclusion,the low energy density prepartum diet was effective in controlling NE_L intake prepartum,and was beneficial in increasing DMI and milk yield,and alleviating negative EB postpartum.     

Effects of body condition score at parturition and postpartum supplemental fat on metabolite and hormone concentrations of beef cows and their suckling calves

To determine the effects of BCS at parturition and postpartum lipid supplementation on blood metabolite and hormone concentrations, 3-yr-old Angus x Gelbvieh beef cows, which were nutritionally managed to achieve a BCS of 4 +/- 0.07 (479.3 +/- 36.3 kg of BW) or 6 +/- 0.07 (579.6 +/- 53.1 kg of BW) at parturition, were used in a 2-yr experiment (n = 36/yr). Beginning at 3 d postpartum, cows within each BCS were assigned randomly to be fed hay and a low-fat control supplement or lipid supplements with either cracked high-linoleate or high-oleate safflower seeds until d 61 of lactation. The diets were formulated to be isonitrogenous and isocaloric, and the safflower seed supplements were formulated to achieve 5% DMI as fat. On d 31 and 61 of lactation, blood samples were collected preprandially and then hourly postprandially (at 0, 1, 2, 3, and 4 h). Serum insulin (P = 0.27) and glucose (P = 0.64) were not affected by BCS at parturition. The mean concentrations of plasma NEFA (P = 0.08) and beta-hydroxybutyrate (P = 0.08) tended to be greater, and serum IGF-I was greater (P < 0.001) in BCS 6 than BCS 4 cows. Conversely, serum GH was greater (P = 0.003) for BCS 4 cows, indicating that regulation of IGF by GH may have been uncoupled in BCS 4 cows. The postpartum diet did not affect NEFA (P = 0.94), glucose (P = 0.15), IGF-I (P = 0.33), or GH (P = 0.62) concentrations. Oleate-supplemented cows had greater (P = 0.03) serum insulin concentrations, whereas control cows had greater (P = 0.01) plasma beta-hydroxybutyrate concentrations. Concentrations of NEFA (P = 0.05) and glucose (P < 0.001) were greater, and beta-hydroxybutyrate tended (P = 0.07), to be greater at d 3, whereas serum IGF-I was greater (P = 0.003) at d 6 of lactation. Similar concentrations of NEFA, glucose, GH, and IGF-I indicate that the nutritional status of beef cows during early lactation was not influenced by lipid supplementation. However, perturbations of the somatotropic axis in BCS 4 cows indicate that the influence of energy balance and BCS of the cow at parturition on postpartum performance should be considered when making managerial decisions.     

Whole sunflower seeds as a source of polyunsaturated fatty acids for grazing dairy cows: Effects on metabolic profiles and resumption of postpartum ovarian cyclicity

Intake of polyunsaturated fatty acids (PUFA) could modify follicular dynamics and reduce the length of the interval from calving to first ovulation (ICOV) in dairy cows, associated with changes in metabolites (i.e. cholesterol) or hormones (i.e. IGF-I). To test this hypothesis, 48 cows were stratified in two groups (primiparous or multiparous) and within each group were randomly assigned to three treatments to begin at calving: 0 kg (SS0), 0.7 kg (SS0.7) and 1.4 kg (SS1.4) of whole sunflower seeds (WSS) per cow per day. The experimental period lasted for 60 days after calving and the diets, which consisted of direct grazing of improved pastures, whole-plant wheat silage and concentrates, were designed to be isocaloric and isoproteic (1.6 Mcal NEL/kg DM, 16.7% CP). Ovaries were examined by ultrasound three times per week from day 8 postpartum (PP) until first PP ovulation. Body condition score (BCS) was determined and blood samples were collected weekly from calving to characterize the metabolic profiles. In treatments SS0.7 and SS1.4, 7/8 and 6/8 primiparous cows ovulated the first follicular wave PP, compared with only 1/8 primiparous cow in treatment SS0, while there were no treatment differences in multiparous cows. The ICOV was 44, 21 and 19 days for the primiparous, and 22, 21 and 25 days for the multiparous cows, in treatments SS0, SS0.7 and SS1.4, respectively. Treatments had no effect on BCS, plasma IGF-I and metabolic profiles except that NEFA and urea were increased by WSS supplementation. Intake of PUFA (WSS up to 1.4 kg/day or 6.7% of the diet) during early lactation reduced the ICOV in primiparous grazing dairy cows in early lactation but not in multiparous cows, and while the precise mechanism for these results is unclear, it was not related to changes in the plasma concentrations of IGF-I or cholesterol.     

Evaluating the suitability of field beans as a substitute for soybean meal in early-lactating dairy cow: Production and metabolic responses

Twenty-four multiparous Holstein cows were utilized in a completely randomized design to examine the effect of feeding field beans (FB, Vicia faba L. var. minor) as substitute for soybean meal (SBM) on lactation performance and metabolic response during the early lactation period. Cows were individually divided into two equal groups and fed for 16 weeks on one each of the two experimental diets. The controls were fed pelleted concentrate contained 150 g/kg dry matter (DM) of SBM as the main protein source, whereas the experimental concentrate contained 345 g/kg DM of FB. Oat hay was offered ad libitum to cows and water was freely available. Blood samples were assayed for their content of: urea, glucose, triglycerides, cholesterol, total protein, albumin, calcium and phosphorus, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA). Inclusion of FB had no detectable effects on DM intake (DMI), body weight (BW), or body condition score (BCS). Neither milk yield nor quality were influenced by dietary treatment, except for milk urea nitrogen (MU) that was reduced in cows fed the FB diet (P < 0.05). Clotting properties of milk were not affected adversely by added dietary FB. Concentration of blood urea (BU) was lower in cows fed the FB diet than in those fed the control SBM diet (P < 0.05). These findings indicate that feeding FB in a lactation diet supported lactation performance similar to cows fed traditional SBM-based diet, and the results may elicit great interest for countries where soybean utilization is adversely influenced by high supply costs.     

Lasalocid Supplementation of Dairy Cow Diets in Early Lactation

A 36-cow study was conducted to evaluate the effects of lasalocid on feed intake, milk production and composition, and various blood metabolites in early lactation. Multiparous Holstein cows were assigned at parturition to a total mixed diet containing 33% corn silage, 17% chopped alfalfa hay, and 50% concentrate mix on a DM basis for 10 wk. An additional 2.3 kg of alfalfa hay was fed during the first 7 d in milk (DIM). Diets were supplemented with 0, 180, or 360 mg/d of lasalocid in a completely randomized design. Covariant-adjusted mean (the covariate was mature equivalent milk production from the previous lactation) DMI; milk and fat-corrected milk (FCM) production; percentages of milk fat, protein, and solids-not-fat; milk somatic cell count; BW; body condition score; and concentrations of glucose, β-hydroxybutyrate, and non-esterified fatty acid (NEFA) in blood were unaffected by supplementation with lasalocid. A dose of 360 mg/d of lasalocid may not be enough to significantly affect high-producing cows consuming >20 kg/d of DM.     

Effect of reduced energy density of close-up diets on dry matter intake,lactation performance and energy balance in multiparous Holstein cows

Energy intake prepartum is critically important to health, milk performance, and profitability of dairy cows. The objective of this study was to determine the effect of reduced energy density of close-up diets on dry matter intake (DMI), lactation performance and energy balance (EB) in multiparous Holstein cows which were housed in a free-stall barn and fed for ad libitum intake. Thirty-nine dry cows were blocked and assigned randomly to three groups fed a high energy density diet [HD, n = 13; 6.8 MJ of net energy for lactation (NE_L)/kg; 14.0% crude protein (CP) ], or a middle energy density diet (MD, n = 13; 6.2 MJ NE_L/kg; 14.0% CP), or a low energy density diet (LD, n = 13; 5.4 MJ NE_L/kg; 14.0% CP) from d 21 before expected day of calving. After parturition, all cows were fed the same lactation diet to d 70 in milk (DIM). The DMI and NE_L intake prepartum were decreased by the reduced energy density diets (P < 0.05). The LD group consumed 1.3 kg/d (DM) more diet compared with HD group in the last 24 h before calving. The milk yield and the postpartum DMI were increased by the reduced energy density diet prepartum (P < 0.05). The changes in BCS and BW prepartum and postpartum were not affected by prepartum diets. HD group had higher milk fat content and lower lactose content compared with LD group during the first 3 wk of lactation (P < 0.05). The energy consumption for HD, MD and LD groups were 149.8%, 126.2% and 101.1% of their calculated energy requirements prepartum (P < 0.05), and 72.7%, 73.1% and 75.2% during the first 4 wk postpartum, respectively. In conclusion, the low energy density prepartum diet was effective in controlling NE_L intake prepartum, and was beneficial in increasing DMI and milk yield, and alleviating negative EB postpartum.     

Influence of body condition at calving and postpartum nutrition on endocrine function and reproductive performance of primiparous beef cows

The influences of body condition score (BCS) at calving and postpartum nutrition on endocrine and ovarian functions, and reproductive performance, were determined by randomly allocating thin (mean BCS = 4.4 +/- 0.1) or moderate condition (mean BCS = 5.1 +/- 0.1) Angus x Hereford primiparous cows to receive one of two nutritional treatments after calving. Cows were fed to gain either 0.45 kg/d (M, n = 17) or 0.90 kg/d (H, n = 17) for the first 71 +/- 3 d postpartum. All cows were then fed the M diet until 21 d after the first estrus. A replication (yr 2; M, n = 25; H, n = 23) was also used to evaluate reproductive characteristics. Concentrations of IGF-I, leptin, insulin, glucose, NEFA, and thyroxine were quantified in plasma samples collected weekly during treatment and during 7 wk before the first estrus. Estrous behavior was detected by radiotelemetry, and luteal activity was determined based on concentrations of progesterone in plasma. All cows were bred by AI between 14 and 20 h after onset of estrus, and pregnancy was assessed at 35 to 55 d after AI by ultrasonography. Cows that calved with a BCS of 4 or 5 had similar endocrine function and reproductive performance at the first estrus. During treatment, H cows gained BW and increased BCS (P < 0.01), and had greater (P < 0.05) concentrations of IGF-I, leptin, insulin, glucose, and thyroxine in plasma than M cows. However, during the 7 wk before the first estrus, plasma concentrations of IGF-I, leptin, insulin, glucose, NEFA, and thyroxine were not affected by time. Cows previously on the H treatment had a shorter (P < 0.01) interval to first postpartum estrus and ovulation, and a larger dominant follicle (P < 0.01) at first estrus, than M cows, but duration of estrus and the number of mounts received were not influenced by nutrient intake. Pregnancy rate at the first estrus was greater (P < 0.03) for H (76%, n = 38) than for M (58%, n = 33) cows. Increased nutrient intake after calving stimulated secretion of anabolic hormones, promoted fat deposition, shortened the postpartum interval to estrus, and increased pregnancy rate at the first estrus. Concentrations of IGF-I and leptin in plasma were constant during 7 wk before the first estrus, indicating that acute changes in these hormones are not associated with the resumption of ovarian function in primiparous beef cows.     

Effects of supplementation of whole corn germ on reproductive performance, calf performance, and leptin concentration in primiparous and mature beef cows

A 2-yr study using primiparous and multiparous, spring-calving, crossbred beef cows was conducted to evaluate the effects of supplemental whole corn germ on reproductive performance, calf performance, and serum leptin concentrations. Each year, cows were blocked by age and BCS and assigned randomly to one of three treatments: PRE (n = 115) cows received 1.14 kg/d (DM basis) of whole corn germ for approximately 45 d before calving; POST (n = 109) cows were fed 1.14 kg/d of whole corn germ for approximately 45 d after calving; and control cows (n = 118) were fed similar energy and protein from dry-rolled corn (1.82 kg of DM/d) for 45 d before and after calving. Additionally, PRE cows were grouped with controls after calving, and POST cows were grouped with control cows before calving, so that corn germ-supplemented cows received the control supplement in the alternate feeding period. Cow BW (538 +/- 13 kg) and BCS (5.4 +/- 0.13) did not differ among treatments at any time during the experiment. Calf birth weight (39 +/- 2 kg), weaning weight (225 +/- 7 kg), and age-adjusted weaning weight (234 +/- 8 kg) did not differ because of dam supplementation regimen. Treatment did not affect the proportion of cows exhibiting ovarian luteal activity before the start of the breeding season (67%) or pregnancy rate (91%). The interval from exposure to bulls until subsequent calving did not differ (P = 0.16) among PRE (298 +/- 2.3 d), POST (303 +/- 2.6 d), and control (304 +/- 2.3 d) cows. Leptin concentrations did not differ among treatments and were 2.15 +/- 0.75, 1.88 +/- 0.76, and 1.91 +/- 0.75 ng/mL for control, POST, and PRE cows, respectively. Age and week relative to calving influenced leptin concentration. Primiparous cows had similar leptin concentrations to 3-yr-old and mature cows for wk -7 and -6 relative to calving, but lower (P < 0.10) concentrations than mature cows for wk -5, and lower (P < 0.05) concentrations than either 3-yr-old or mature cows for wk -4 to +7 relative to calving. Serum leptin was correlated with BCS (P < 0.0001; r = 0.35) at initiation of the feeding period and was correlated with BCS (P = 0.02; r = 0.12) and weight (P < 0.01; r = 0.14) at the completion of the supplement period, but it was not correlated with initial BW or interim BCS. Calving interval was not correlated (P > 0.12) with weekly measures of serum leptin concentration. Supplementing beef cows with whole corn germ had no effect on cow performance, calf performance, or serum leptin concentrations of cows.     

Rumen-protected choline administration to transition cows: effects on milk production and vitamin E status

We investigated the influence of rumen-protected choline (RPC) supplementation on milk production, lipid metabolism and vitamin E status in dairy cows receiving a silage-based diet. Twenty-six Italian Holstein multiparous cows were assigned by weight and average production in the previous lactation, to one of two groups: control (no RPC supplementation) and RPC (supplemented with 20 g/day rumen-protected choline chloride). Treatment began 14 days before expected calving and continued for 30 days after parturition. Choline administration significantly increased milk production during the first month of lactation and also the concentration (and total secretion) of choline in milk, but did not affect fat or protein concentrations in milk, or plasma levels of glucose, beta-hydroxybutyrate, cholesterol and non-esterified fatty acids (NEFA). However, around parturition, NEFA concentrations in plasma were lower in treated animals than in controls, suggesting improved lipid metabolism as a result of choline supplementation. Choline supplementation also increased alpha-tocopherol plasma concentrations, suggesting a novel aspect in dairy cows.     

Effects of body condition at far-off dry period on blood biochemistry,liver triacylglycerol and muscular monocarboxylate transporter-1 mRNA expression in tropical Holstein dairy cows during peripartum period

Effects of body condition score (BCS) at far-off dry period on blood biochemistry, liver triacylglycerol, and muscular monocarboxylate transporter-1 mRNA expression in tropical Holstein dairy cows during peripartum period were studied. Eight dry cows were divided into two groups of four cows based on their BCS at 8 weeks prepartum: high BCS (HBS) and low BCS (LBS). Blood samples were collected weekly from 8 weeks before expected calving date until 8 weeks postpartum for determination of serum glucose, non-esterified fatty acid (NEFA), and β-hydroxybutyrate (BHBA). Muscle and liver samples were biopsied at 2 weeks before expected calving date and at 2 weeks postpartum for determination of muscular monocarboxylate transporter-1 (MCT-1) mRNA expression and liver triacylglycerol concentrations. Decreased BCS and elevated serum NEFA and BHBA indicated that LBS cows entered to the NEB period earlier than did HBS cows. LBS cows showed lower liver TAG and higher muscular MCT-1 mRNA expression than did HBS cows. The MCT-1 mRNA expression at 2 weeks prepartum was negatively correlated with BCSs at 2–3 weeks prepartum and at 1–2 weeks postpartum. In conclusion, LBS cows most likely adapted to NEB by upregulation of muscular MCT-1 expression and had lower NEB consequences during postpartum period.     

Responses of energy balance, physiology, and production for transition dairy cows fed with a low-energy prepartum diet during hot season

Twenty multiparous Chinese Holstein dairy cows calving in hot summer (S group), were compared with 20 similar control cows calving in cool autumn (C group). Diets were the same for both groups; prepartum diets had relatively low energy density. Average temperature–humidity index was 76.5 and 53.0 in summer and autumn, respectively. S group cows had significantly higher rectal temperatures (39.6 vs. 39.0 °C) and respiration rates (79.0 vs. 31.3 breaths/min) than C group, and consumed less feed (prepartum 8.0 vs. 12.3 kg/day, postpartum 16.3 vs. 21.2 kg/day). Calculated energy balance (EB) was ?7.98 vs. ?5.15 Mcal/day for S group prepartum and postpartum, respectively. In contrast, EB was 1.36 vs. ?2.03 Mcal/day for C group prepartum and postpartum, respectively. S group produced significantly less milk than C group by 15.4 % (5.2 kg/day) and 26.8 % (10.2 kg/d) for milk yield and energy-corrected milk, respectively. Percentages of milk fat (3.28 vs. 4.29 %), protein (3.08 vs. 3.33 %), and solids-not-fat (8.46 vs. 8.78 %) were significantly lower for S group. Milk urea nitrogen (19.54 vs. 13.31 mg/dL) was significantly higher in S group. Significantly lower feed efficiency was observed in S group (1.56 vs. 1.66). During the entire transition period, S group had significantly lower circulating glucose levels. S group had significantly higher levels of nonesterified fatty acids (NEFA) prepartum, but after 14 days in milk, NEFA was significantly lower. We conclude that increasing dietary energy density during transition period (especially prepartum) is necessary to minimize adverse effects of hot season.     

Studies of the transition cow under a pasture-based milk production system: metabolic profiles

This study describes the effect of parity (multiparous versus primiparous) and body condition score (BCS) at calving (<3 or > or =3; scale 1-5) on variations of BCS, body weight (BW) and metabolic profiles in Holstein cows grazing on improved pastures. Forty-two cows were studied (21 multiparous and 21 primiparous) from 2 months before to 3 months after calving. BCS, BW and milk production were measured every 2 weeks. Blood samples were taken every 2 weeks to determine total protein, albumin, urea, non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), cholesterol, aspartate aminotransferase (AST), calcium, phosphorus and magnesium. Primiparous cows had lower BCS during the early postpartum (PP) period and produced less milk than multiparous. In primiparous cows NEFA concentrations were higher during the early postpartum period; BHB levels were similar in both categories during this period. Primiparous cows showed a more unbalanced metabolic profile than multiparous cows, reflecting that they are recovering from the loss of BCS after calving with less success.     

Effect of Prepartum Energetic Supplementation on Productive and Reproductive Characteristics, and Metabolic and Hormonal Profiles in Dairy Cows under Grazing Conditions

The effect of cracked corn grain supplementation (3.5 kg/day) during 3 weeks before the expected calving date on milk production and composition, body condition score (BCS), metabolic and hormonal profiles and length of postpartum anoestrus was evaluated in multiparous Holstein dairy cows under grazing conditions (Energy supplemented group, n = 10; Control group, n = 10). Body condition score was weekly recorded during the peripartum period, from days −21 to +35 (parturition = day 0). Non-esterified fatty acids, β-hydroxybutyrate, cholesterol, urea, insulin, insulin-like growth factor I (IGF-I), leptin, thyroxine (T₄) and 3,3'5-triiodothyroinine (T₃) were weekly determined in plasma from days −21 to +35. The reinitiation of ovarian cyclicity was twice weekly determined by ovarian ultrasonography and confirmed by plasma progesterone concentrations. Cows fed energy concentrate prepartum had higher BCS during the prepartum and postpartum and produced more milk. Non-esterified fatty acids plasma concentrations were significantly higher in the energy group, while cholesterol was higher in the control group. Treated cows had higher levels of plasma insulin, IGF-I and leptin pre-calving. IGF-I, leptin and T₄ were diminished during the early postpartum period in both groups. Insulin levels were also diminished in the control group, but levels remained high in the energy-supplemented group. Treated cows ovulated sooner after parturition than controls. We conclude that Energetic supplementation prepartum in cows under grazing conditions increased milk production and reduced the reinitiation of ovarian activity, consistent with a better EB (BCS), higher prepartum levels of IGF-I, leptin and insulin, and higher insulin levels during early postpartum.     

Effects of malic acid on feed intake, milk yield, milk components and metabolites in early lactation Holstein dairy cows

The objective of this study was to evaluate the effects of malic acid (MA) on feed intake, milk yield and composition, blood metabolites and energy balance in early lactation Holstein dairy cows from 1 to 63 day in milk (DIM). Twenty-eight multiparous Holstein dairy cows, blocked by lactation number, previous 305-d mature equivalent milk production, and expected calving date, were arranged into four groups in a randomized block design. Treatments were: control (without MA), LMA, MMA and HMA with 70, 140 and 210 g malic acid per cow per day, respectively. The supplement of food grade MA (99.8% of MA) was hand-mixed into the top one-third of the daily ration. Cows were fed ad libitum a total mixed ration consisting of equal proportion of forage and concentrate. Milk yield increased (P = 0.04), but feed intake and milk components were not affected (P > 0.05) by MA supplementation. The energy balance, expressed as the difference between energy input and output, tended to be higher (P = 0.08) for MA supplemented cows during the 63-DIM period MA and supplemented cows showed a trend (P = 0.07) toward less loss of BW during the 63-day period, especially during the first 21-day of lactation. Concentrations of plasma glucose and serum insulin were higher for cows fed LMA, MMA, and HMA relative to control and linearly (P < 0.01) increased with increasing MA supplementation. Concentrations of non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHBA) and urine ketones were lower for MA-supplemented cows at 7, 14 and 21 DIM of lactation and linearly (P < 0.01) decreased with increasing MA supplementation. Although feed intake was not affected, milk yield increased, MA-supplemented cows experienced higher concentrations of plasma glucose and serum insulin, lower concentrations of plasma BHBA and NEFA, and lower concentrations of urine ketones, suggesting that nutrient digestibilities and energy availability may have been improved.     

Pre-partum monensin supplementation improves body reserves at calving and milk yield in Holstein cows dried-off with low body condition score

The objective of this study was to determine the effect of a monensin controlled-release capsule administered intraruminally at drying-off on body condition score (BCS) at calving, milk yield, fertility and concentration of energy-related blood metabolites in Holstein cows dried-off with low BCS (< or = 3.0, scale 1 to 5 with a 0.25 point of increment). Between July and August, 2001, 220 cows from parity 2 or more and dried-off 50-70 days before expected parturition, with a BCS < or = 3.0 were randomly assigned to either a treatment group (n=110; oral capsule of monensin releasing 335 mg/day for 95 days) or a control group (no capsule, n=110). At assignment, on day 21 before expected parturition, at calving, and at 7, 14, and 21 days in milk a blood sample was obtained from a random sub sample of 10 cows per group. Effects of monensin on serum NEFA, BHBA and glucose were measured. Milk yield, milk fat and protein content (%) at DHIA test days during the entire lactation, 305 ME milk production and reproductive responses were compared. Monensin significantly improved BCS at calving, increased milk yield at test days 4 and 8, decreased the percentage of milk protein, did not change the percentage of milk fat, and decreased NEFA and BHBA during the post-partum period.     

Use of Anionic Salts with Grazing Prepartum Dairy Cows

Twenty multiparous and four primigravid Holstein cows were utilized in a completely random design to characterize the influence of decreasing prepartum dietary cation-anion difference (DCAD) from +388 to +183 meq/kg diet on DMI, prepartum blood profiles, and postpartum milk yield and composition of dairy cows grazed during the prepartum period. Treatments began on wk −4 prepartum, continued through calving, and consisted of 1) pasture and grain pellet without anionic salts (control; +388 meq/kg) or 2) pasture and grain pellet containing anionic salts (AS) (+183 meq/kg). Prepartum cows were rotationally grazed as a single group and individually fed pellets twice daily at a rate of 0.5% of BW/d. Blood and urine samples were collected on wk −4, −2.5, and −1 prepartum and analyzed for Ca, Mg, K, Na, and Cl concentrations. Urine samples were also analyzed for pH. Chromic oxide was dosed twice daily during the last 4 wk of gestation and again for 10 d during wk 4 and 12 postpartum for estimation of intake. Cows calved on pasture and were then integrated into the regular milking herd and fed a total mixed ration (TMR). Daily milk yield and weekly milk samples were collected through wk 14 of lactation. Prepartum and postpartum DMI, milk yield and composition, and plasma minerals were not affected by treatment. No clinical cases of milk fever were observed for either treatment group. Reducing prepartum DCAD from +388 to +183 meq/kg DM did not improve prepartum blood profiles or postpartum milk yield or composition; therefore, this type of supplementation was not economical.     

Supplementation of dried distillers grains with solubles to beef cows consuming low-quality forage during late gestation and early lactation

Three experiments were conducted to evaluate supplementation of dried distillers grains with solubles (DGS) to spring-calving beef cows (n = 120; 541 kg of initial BW; 5.1 initial BCS) consuming low-quality forage during late gestation and early lactation. Supplemental treatments included (DM basis) 1) 0.77 kg/d DGS (DGSL); 2) 1.54 kg/d DGS (DGSI); 3) 2.31 kg/d DGS (DGSH); 4) 1.54 kg/d of a blend of 49% wheat middlings and 51% cottonseed meal (POS); and 5) 0.23 kg/d of a cottonseed hull-based pellet (NEG). Feeding rate and CP intake were similar for DGSI and POS. In Exp. 1, cows were individually fed 3 d/wk until calving and 4 d/wk during lactation; total supplementation period was 119 d, encompassing 106 d of gestation and 13 d of lactation. Tall-grass prairie hay (5.6% CP, 50% TDN, 73% NDF; DM basis) was fed for ad libitum intake throughout the supplementation period. Change in cow BW and BCS during gestation was similar for DGSI and POS (-5.0 kg, P = 0.61 and -0.13, P = 0.25, respectively) and linearly increased with increasing DGS level (P < 0.01). Likewise, during the 119-d supplementation period, BW and BCS change were similar for DGSI and POS (-72 kg, P = 0.22 and -0.60, P = 0.10) and increased linearly with respect to increasing DGS (P < 0.01). The percentage of cows exhibiting luteal activity at the beginning of breeding season (56%, P = 0.31), AI conception rate (57%, P = 0.62), or pregnancy rate at weaning (88%, P = 0.74) were not influenced by supplementation. In Exp. 2, 30 cows from a separate herd were used to evaluate the effect of DGS on hay intake and digestion. Supplementation improved all digestibility measures compared with NEG. Hay intake was not influenced by DGS (P > 0.10); digestibility of NDF, ADF, CP, and fat linearly increased with increasing DGS. In Exp. 3, milk production and composition were determined for cows (n = 16/treatment) of similar days postpartum from Exp. 1. Daily milk production was not influenced by supplementation (6.3 kg/d, P = 0.25). Milk fat (2.1%) and lactose (5.0%) were not different (P > 0.10). Milk protein linearly increased as DGS increased (P < 0.05) and was greater for DGSI compared with POS. Similar cow performance was achieved when cows were fed DGS at the same rate and level of CP as a traditional cottonseed meal-based supplement. Increasing amounts of DGS did not negatively influence forage intake or diet digestibility.