Replacing corn with pearl millet (raw and sprouted) with and without enzyme in chickens' diet

An experiment was conducted to compare a commercial corn–soya bean meal diet with a pearl millet (raw and sprouted) diet containing less soya bean meal, alone or in combination with exogenous enzyme, on growth performance and ileal villus development of chicks. Two‐hundred‐and‐forty‐one‐day‐old male broilers (10/pen) were randomly allocated to one of the following dietary treatments: (i) a standard corn–soya bean meal control diet (CTL); (ii) a raw pearl millet‐soya bean meal diet (PM); (iii) a sprouted pearl millet‐soya bean meal diet (SPM); (iv) CTL + exogenous enzymes (CE); (v) PM + exogenous enzymes (PE); and (vi) SPM + exogenous enzymes (SPE) with four replicate pens/treatment. Body weight of birds at day 21 did not differ between those fed the CTL, and SPM and PE diets. In comparison with feeding broilers the CTL diet, feeding the PE and SPM diets caused significant decrease in feed intake, but with equivalent growth and feed efficiency. However, at day 21, feed conversion ratio did not differ between birds fed the CTL diet and those fed the PM, PE and SPM diets. At day 21, broilers fed the PM and PE diets had longer villi (p < 0.05) than those fed the CTL diet. At day 21, villi width was reduced (p < 0.05) by raw pearl millet supplementation than CTL diet. It is concluded that, in comparison with corn, broiler diets formulated with sprouted pearl millet or pearl millet with enzyme require less soya bean meal and can be used to improve growth performance traits and villus development.     

Additivity of values for apparent and standardized ileal digestibility of amino acids in mixed diets fed to growing pigs

The objective of this experiment was to determine whether the digestibility of CP and AA in a mixed diet fed to growing pigs is better predicted when based on standardized ileal digestibility coefficients (SID) or apparent ileal digestibility coefficients (AID). Eight growing pigs (initial BW = 92.1 +/- 3.19 kg) were surgically equipped with a T-cannula in the distal ileum and arranged in an 8 x 8 Latin square design with eight diets and eight periods. Three of the diets contained corn, soybean meal (SBM), or canola meal (CM) as the sole source of CP and AA. Four mixed diets also were formulated using corn and soybean meal (CS); corn and canola meal (CCM); soybean meal and canola meal (SCM); or corn, soybean meal, and canola meal (CSCM). A N-free diet was used to measure the basal ileal endogenous losses (IAAend) of CP and AA. Pigs were fed each of the eight diets during one 7-d period, and ileal digesta were collected during two 10-h periods on d 6 and 7. The AID values were calculated for CP and AA in all diets, except the N-free diet. By correcting the AID for IAAend, the SID for CP and AA in each of the seven protein-containing diets were calculated. As expected, the AID for CP and the majority of AA were greater in SBM than in corn and CM (P < 0.05); however, the SID for CP and most AA did not differ between corn and SBM. For the majority of the AA, SID were less (P < 0.05) in CM than in the other two ingredients. Using the AID and the SID that were measured for CP and AA in corn, SBM, and CM, the AID and the SID in the four mixed diets were predicted and compared with the measured values for these diets. For the three mixed diets containing corn, the measured AID for CP and most AA were greater (P < 0.05) than the predicted AID, but with a few exceptions, no differences between predicted and measured values for SID were observed. For the diet based on SCM, there were no differences between predicted and measured values regardless of the procedure used, except for the AID of Ser. The results of this experiment demonstrate that the digestibility coefficients for a mixed diet containing low-protein feed ingredients, such as corn, are more accurately predicted using SID than AID.     

Effect of phytase supplementation to diets for weanling pigs on the digestibilities of crude protein, amino acids, and energy

Four experiments were conducted with weanling pigs fitted with a simple T-cannula at the distal ileum, to determine the effect of phytase supplementation to four diets on the apparent ileal digestibilities (AID) of CP and AA, and the apparent total-tract digestibilities (ATTD) of CP and DE. Phytase (Natuphos, DSM Food Specialties, Delft, The Netherlands) was supplemented at rates of 0, 500 or 1,000 FTU/kg to the four diets. A 20% CP (as-fed basis) corn-soybean meal diet was used in Exp. 1; a 20% CP wheat-soybean meal diet in Exp. 2; a 20% CP wheat-soybean meal-canola meal diet in Exp. 3; and a 19% CP barley-peas-canola meal diet in Exp. 4. In each experiment, six barrows, fitted with a simple T-cannula at the distal ileum, were fed the basal plus phytase-supplemented diets according to a repeated 3 x 3 Latin square design. Each experimental period comprised 14 d. The piglets were at fed 0800 and 2000 daily, equal amounts for each meal, at a daily rate of at least 2.4 times the maintenance requirement for ME. Feces were collected from 0800 on d 8 until 0800 on d 12 of each experimental period. Ileal digesta were collected from 0800 to 2000 on d 12, 13, and 14. Chromic oxide was used as the digestibility marker. The average initial and final BW (average of all experiments) were 7.9 and 16.5 kg, respectively. Phytase supplementation did not improve the AID of CP and AA in Exp. 1, 2, and 4; however, there were improvements (P < 0.05) or tendencies (P < 0.10) toward improvements in the AID of CP and AA or the ATTD of CP and the content of DE with phytase supplementation in Exp. 3. These results suggest that the AA response factor to microbial phytase supplementation depends on diet composition.     

Calcium, phosphorus, and amino acid digestibility in low-phytate corn, normal corn, and soybean meal by growing pigs

Nine growing barrows were equipped with a T-cannula in the distal ileum and used to determine apparent ileal (AID) and apparent total-tract digestibility (ATTD) coefficients of Ca and P in low-phytate corn, normal corn, soybean meal, and in diets where soybean meal was mixed with low-phytate corn or normal corn. The AID and the standardized ileal digestibility coefficients (SID) of CP and AA also were determined. The animals (initial BW = 29.3 +/- 1 kg) were allotted to a 9 x 9 Latin square with nine diets and nine periods. Three diets contained low-phytate corn, normal corn, and soybean meal as their sole source of CP, AA, Ca, and P, respectively. Three additional diets were identical to these diets except that limestone and monosodium phosphate were added. Two diets contained low-phytate corn or normal corn and soybean meal, limestone, and monosodium phosphate, and the final diet was a N-free diet. The AID and ATTD of Ca were higher (P < 0.05) for low-phytate corn than for normal corn (70.0 and 69.1% vs. 47.4 and 49.6%, respectively). The AID and ATTD for Ca in soybean meal (50.9 and 46.7%, respectively) did not differ from values for normal corn but were lower (P < 0.05) than for low-phytate corn. The AID and ATTD for P from low-phytate corn (56.5 and 54.5%, respectively) were greater (P < 0.05) than from normal corn (28.3 and 28.8%, respectively), whereas soybean meal had intermediate AID and ATTD for P (37.2 and 38.0%, respectively). The AID and ATTD of P increased (P < 0.05) when monosodium phosphate was added to normal corn (44.9 and 49.8%, respectively) and soybean meal (49.6 and 46.2%, respectively), but adding monosodium phosphate to low-phytate corn, did not alter either AID (49.7%) or ATTD (50.7%) of P. No differences between AID and ATTD for Ca or P within the same diet were observed. The AID of Arg, Asp, Gly, Ile, Lys, Phe, Thr, and Val were greater (P < 0.05) in low-phytate corn than in normal corn. The AID of all AA in soybean meal were greater (P < 0.05) than in both types of corn, with the exception of Ala, Cys, Leu, and Met. The SID of Lys, Phe, and Thr were higher (P < 0.05) in low-phytate corn than in normal corn. Because low-phytate corn has a higher digestibility of Ca and P, less inorganic Ca and P need to be supplemented to diets containing low-phytate corn than to those containing normal corn, and P excretion may be decreased when low-phytate corn is used in the diet.     

Influence of guanidination on apparent ileal digestibility of amino acids in pigs fed diets with soybean meal, rapeseed meal or peas as a protein source

The objective of this study was to determine the effect of guanidination, the conversion of dietary lysine to homoarginine, on the apparent ileal digestibilities (AID) of amino acids (AA) from 3 protein sources in diets for pigs. Six barrows, initial body weight of 18 ± 1.5 kg, fitted with a simple T-cannula at the distal ileum, were fed one of three corn starch-based diets that contained 18% crude protein (CP) from soybean meal, rapeseed meal or peas for an experimental period of 10 d according to a repeated 3 × 3 Latin square design. On d 9 of each experimental period, the pigs were fed one meal of the guanidinated test meals, which corresponded to their respective diets. In the test meals, 50% of the protein source in each diet was replaced, on an equal weight basis, with a guanidinated source. Guanidination increased the content of CP by 190, 116 and 28 g kg^− 1 for soybean meal, rapeseed meal and peas, respectively. With the exception of lysine, the AID of CP and AA were 2.2 to 8.2 percentage units (pu) higher in the test meal than in the soybean meal diet. Other than arginine and isoleucine, the differences were significant (P < 0.05) for all indispensable AA. With the exception of lysine, the AID of CP and AA were 3.8 to 11.9 pu higher in the test meal than in the diet with peas. The differences were significant (P < 0.05) for all indispensable AA. Other than lysine, glycine and proline, the AID of CP and AA were slightly higher, 0.1 to 4.3 pu, in the test meal than in the rapeseed meal diet. Of the indispensable AA, the differences were significant (P < 0.05) for arginine, leucine and phenylalanine. The AID of lysine was 10.9 pu lower (P < 0.05) in the test meal than in the rapeseed meal diet, 61.5 vs. 72.4%, indicating that guanidination of rapeseed meal was not uniform and random. The increase in the AID of CP and AA after guanidination may result from solubilisation of protein and degradation of antinutritional factors such as fiber, trypsin inhibitors and tannins.     

Effect of feed intake on endogenous losses and amino acid and energy digestibility by growing pigs

An experiment was conducted to determine the effect of feed intake (FI) on endogenous losses and the digestibility of CP and AA by growing pigs. Six growing barrows (initial BW = 70.3 kg) had a T-cannula installed in the distal ileum and were used in a 6 x 6 Latin square design. A soybean meal cornstarch-based diet and a N-free diet were formulated. Chromic oxide (0.25%; as-fed basis) was included in both diets as an inert marker. Each diet was provided at three different levels of FI. Feed intake level 1 was equal to the estimated energy requirement for maintenance of the pigs, whereas levels 2 and 3 were two or three times this amount, respectively. Each experimental period lasted 7 d. The initial 4 d of each period comprised an adaptation period to the experimental diets. On d 5, fecal samples were collected, while ileal digesta were collected during two 10-h periods on d 6 and 7. Between each experimental period, a corn-soybean meal-based diet (16% CP) was fed to all pigs for 7 d. The basal ileal endogenous losses (IAA(end)) of CP and AA were measured for each level of FI from pigs fed the N-free diet. Likewise, the apparent (AID) and standardized (SID) ileal digestibility coefficients for CP and AA in soybean meal were calculated for each level of FI. The total-tract digestibility coefficient of energy in the soybean meal-based diet also was calculated. The AID for CP and all indispensable AA except Lys, Met, and Thr increased (P < or =0.055) as FI increased. The IAA(end) of CP and all AA except Pro decreased linearly (P < 0.05) as FI increased when expressed as g/kg of DMI; however, the total daily IAA(end) increased as FI increased (linear, P < or =0.056) for all AA, except for Phe, Thr, Trp, Val, Cys, Gly, and Ser. The AA composition (% of CP) of endogenous protein was not affected by the level of FI, except for Arg, Thr, Pro, and Ser. As FI increased, the SID decreased linearly (P < 0.04) for CP and all AA, except Arg, Trp, Asp, Pro, and Tyr. The total-tract digestibility of energy was not influenced by the FI level. These results demonstrate that the FI level significantly influenced AID, SID, and IAA(end) for CP and AA. Therefore, pigs used to measure AA digestibility coefficients and IAA(end) should be fed at a level that is close to what is used under commercial conditions.     

Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig

Two experiments were conducted to determine the effect of soybean meal in postweaning diets on villus height (VH), villus shape and lamina propria depth (LPD) in the small intestine. Intestinal samples were taken at 25, 50 and 75% of the small intestine's length. The first experiment determined the normal effects of age on these structures in nursing pigs from 9 to 36 d of age. Villus height decreased (P less than .01 at the 50 and 75% locations) and LPD increased (P less than .01 at the 25% location) in a quadratic fashion with increasing age. In the second experiment, pigs were weaned at 21 d of age and fed one of three 20% protein diets: 1) protein from hydrolyzed casein (CAS), 2) soybean meal (SBM) or 3) a conventional corn-soybean meal diet (CSBM). Intestinal samples were taken 0, 3, 6, 9, 12 and 15 d post-weaning. Villus height decreased dramatically the first 3 d postweaning, with a continuing decline until 12 d post-weaning. Averaged over the d 3 to 15 interval, VH were generally greater for pigs fed CAS than for pigs fed CSBM or SBM. Pigs fed SBM or CSBM had deformed villi, and pigs fed SBM had greater (P less than .05) LPD at all intestinal locations, suggesting less mature enterocytes on the villi. These data suggest that feeding high concentrations of soybean meal to the pig post-weaning has a detrimental effect on the small intestine, but this effect was minimized when soybean meal was fed as part of a corn-based diet.     

Apparent and standardized ileal digestibilities of amino acids for pigs fed corn- and soybean meal-based diets at varying crude protein levels

The objective of this study was to determine the effect of CP level in corn- and soybean meal-based diets on apparent (AID) and standardized ileal digestibility (SID) of AA. Six pigs (initial BW, 47.1 ± 1.0 kg) fitted with T-cannula at the distal ileum were fed 6 diets for 6 periods in a 6 × 6 Latin square design. The 6 diets consisted of a nitrogen-free diet and 5 corn- and soybean meal-based diets that contained CP of 68, 105, 141, 177, and 214 g/kg. Each period consisted of a 5-d adjustment period and 2 d of ileal digesta collection for 10 h on each of d 6 and 7. The ratio of corn:soybean meal was fixed at 3 to 2 by weight and cornstarch was added to dilute the CP concentration. Chromic oxide was added at 5 g/kg as an indigestible marker. The results showed basal endogenous loss ranged from 65 mg/kg of DMI for Met to 3,104 mg/kg of DMI for Pro. Proline and Gly (1,053 mg/kg of DMI) were the 2 most abundant AA in endogenous flow and together accounted for approximately 43% of the total endogenous AA flow. Of the basal ileal endogenous CP, total AA accounted for 82%. The AID were 80.9 to 84.7%, 85.1 to 87.4%, 72.9 to 79.5%, and 86.5 to 87.9% for Lys, Met, Thr, and Trp, respectively, with corresponding SID being 86.6 to 89.0%, 87.5 to 90.5%, 82.7 to 88.2%, and 90.2 to 94.6%, respectively, as dietary CP increased from 68 to 214 g/kg. There were linear increases in AID of N, Arg, Gly, Ile, Lys, Ser, Thr, Tyr, and Val (P ≤ 0.05) as CP increased and linear decreases in SID of N and all AA measured in this study except Lys, Met, and Pro (P ≤ 0.05). Both linear and quadratic effects were observed in AID for Pro (P < 0.05). In conclusion, the protein content of corn-soybean meal diets evaluated in the current study affected SID of most indispensable and dispensable AA, excluding Lys, Met, and Pro.     

Effect of dietary soybean oil and soybean protein concentration on the concentration of digestible amino acids in soybean products fed to growing pigs

An experiment was conducted to measure the effect of adding soybean oil to soybean meal (SBM) and soy protein concentrate (SPC) on apparent (AID) and standardized (SID) ileal digestibility of CP and AA by growing pigs. A second objective was to compare AID and SID of AA in a new high-protein variety of full fat soybeans (FFSB) to values obtained in other soybean products. Commercial sources of FFSB (FFSB-CV), SBM, and SPC, and of a new high-protein variety of FFSB (FFSB-HP) were used in the experiment. Four diets were prepared using each soybean product as the sole source of CP and AA in 1 diet. Two additional diets were formulated by adding soybean oil (7.55 and 7.35%, respectively) to the diets containing SBM and SPC. A nitrogen-free diet was also used to measure basal endogenous losses of CP and AA. The 2 sources of FFSB were extruded at 150 degrees C before being used in the experiment. Seven growing barrows (initial BW = 26.2 kg) were prepared with a T-cannula in the distal ileum and allotted to a 7 x 7 Latin square design. Ileal digesta were collected from the pigs on d 6 and 7 of each period. All digesta samples were lyophilized and analyzed for DM, CP, AA, and chromium, and values for AID and SID of CP and AA were calculated. The addition of oil improved (P < 0.05) the SID of most indispensable AA in SBM and SPC. The SID for 6 of the indispensable AA in FFSB-HP were greater (P < 0.05) than in FFSB-CV, and the SID for all indispensable AA except Met was greater (P < 0.05) in FFSB-HP than in SBM. However, the SID for most AA in FFSB-HP was similar to SBM with oil and SPC, but these values were lower (P < 0.05) than in SPC with oil. In conclusion, the addition of oil improved the SID of most AA in SBM and SPC fed to growing pigs, and the SID of AA in FFSB-HP were greater than in SBM and similar to the SID of AA in SBM with oil and in SPC.     

Transient hypersensitivity to soybean meal in the early-weaned pig

An experiment was conducted to determine whether baby pigs develop hypersensitivity to dietary soybean proteins. Thirty-two pigs were orally infused with either dried skim milk (5 g/d; control) or soybean meal (48% CP; 5 g/d) from d 7 to 14 after birth. Sows were fed a corn-corn gluten meal-based diet supplemented with lysine and tryptophan to avoid exposure of pigs to soybean proteins. Pigs were weaned at 21 d of age and fed diets containing either soybean meal or milk proteins until d 56. One half of the pigs were killed at 28 d of age and the rest at 56 d of age. Segments of small intestine were collected, and intraepithelial lymphocytes were isolated. At 28 d of age, pigs fed diets containing soybean meal had lower (P less than .05) villus height (221 vs 298 microns) and rate of gain (86 vs 204 g/d) than control pigs did. Pigs fed a diet containing soybean meal had higher (P less than .05) immunoglobulin G (IgG) titers to soybean protein than did pigs fed a milk protein-based diet. Blood and intestinal lymphocytes collected on d 28 and 56 did not exhibit any proliferative response when cultured with purified soy proteins (2.5 or 5 microns/ml). Phytohemagglutinin- and pokeweed mitogen-induced lymphocyte proliferations were higher (P less than .05) at d 56 than at d 28, but there were no differences attributable to protein source. There were no differences (P greater than .05) in skin-fold thickness measurements following intradermal injection with soy or milk proteins. Decreased villus height and increased serum IgG titers to soybean proteins coinciding with inferior performance of early weaned pigs fed diets containing soybean meal indicate that conventionally processed, commercial soybean meal may retain some antigens that can cause transient hypersensitivity in piglets.     

Nutritive characteristics of pearl millet grain in beef cattle diets

Two trials were conducted to compare pearl millet grain with corn and grain sorghum in cattle diets. Grain portions of diets in metabolism and feedlot experiments contained 73% corn and 6% soybean meal (C); 76.2% grain sorghum and 2.8% soybean meal (GS); and 79% pearl millet (PM). In the metabolism trial (replicated 3 x 3 latin squares; six steers), apparent digestibilities of DM and OM were higher (P less than .05) for C than for GS or PM diets. Ether extract and CP digestibilities were higher (P less than .05) for C and PM than GS. Dietary TDN was higher (P less than .05) for C compared with GS or PM diets. Fecal N was higher (P less than .05) for GS than for C or PM, urinary N was higher (P less than .05) for PM than C or GS, but retained N was similar (P greater than .05) for C, GS and PM diets. In a 90-d feedlot trial (18 individually fed heifers), a trend was observed for higher (P less than .20) ADG on C compared with PM; however, feed/gain was similar for C, GS and PM diets (8.2, 9.1 and 8.5 kg feed/kg gain, respectively). Ruminal fluid acetate:propionate ratios were lower (P less than .05) at 3.5 and 7 h postfeeding for PM compared with C or GS diets on d 83. Pearl millet grain may be used as a feed grain for beef cattle, but diets should be formulated to efficiently utilize the high quantity of protein (about 14% of DM) in this grain as a substitute for supplemental protein.     

蛋氨酸铬对肉鸡生长、脏器指数及血液生化指标的影响

试验旨在研究日粮中添加蛋氨酸铬对肉鸡生长、脏器指数及血清生化指标的影响,为确定日粮中蛋氨酸铬的适宜添加量提供科学依据。试验选用1日龄AA肉鸡528只,设4个处理,每个处理4个重复,每个重复33只。对照组饲喂玉米-豆粕型基础日粮,其它处理组饲喂分别在基础日粮中添加蛋氨酸铬0.1 mg/kg(Ⅰ组)、0.2 mg/kg(Ⅱ组)、0.6 mg/kg(Ⅲ组)的日粮(以Cr3+计),试验期49 d。结果表明:蛋氨酸铬能显著降低饲料消耗(P<0.05),但对肉鸡各日龄段平均体重、料重比和成活率影响差异均不显著(P>0.05)。除能显著降低心脏相对重量(P<0.05)外,日粮中添加蛋氨酸铬对肉鸡其它脏器相对重量影响差异均不显著(P>0.05)。日粮中添加蛋氨酸铬对血液生化指标影响显著(P<0.05)。因此,日粮中补充蛋氨酸铬对肉鸡生产性能、脏器指数影响不明显;但对血液生化指标影响显著,建议日粮中蛋氨酸铬添加量以0.2 mg/kg为宜。     

Effect of phytase supplementation to a low- and a high-phytate diet for growing pigs on the digestibilities of crude protein, amino acids, and energy

Supplementation of microbial phytase usually improves the digestibility and utilization of phosphorus in feedstuffs of plant origin. The effect of phytase supplementation on the digestibilities of AA also has been examined, but the results have been inconsistent. This study was carried out to determine the effect of phytase (Natuphos) supplementation, at a rate of 2,000 phytase units/kg, to two basal diets on the apparent ileal digestibilities (AID) of GE, CP, and AA, and on the apparent total-tract digestibilities (ATTD) of CP and GE. The basal diets contained 18% CP and were formulated (as-fed basis) to contain either a low (0.22%) or high content (0.48%) of phytate P. The high-phytate diet contained 20% rice bran, which is a rich source of phytate and has low intrinsic phytase activity. Eight barrows (average initial BW = 40.6 kg), fitted with a simple T-cannula at the distal ileum, were fed the four diets according to a replicated 4 x 4 Latin square design. The pigs were fed twice daily at 0800 and 2000, equal amounts each meal, at a rate of 2.4 times the daily maintenance requirement for ME. Each experimental period comprised 14 d. Ileal digesta were collected from 0800 to 2000 on d 12, 13, and 14. Feces were collected from 0800 on d 8 until 0800 on d 12. Chromic oxide was used as the digestibility marker. The AID of GE, CP, and AA and the ATTD of CP and GE were less in the high- than in the low-phytate diet (P < 0.01). With the exception of glutamic acid, phytase supplementation did not affect (P > 0.10) the AID of CP and AA. There was no effect (P > 0.05) of phytase on the ATTD of CP and GE. These results show that if a response occurs to phytase supplementation, it is independent of the dietary phytate content.     

Apparent ileal digestibility of amino acids and the digestible and metabolizable energy content of dry extruded-expelled soybean meal and its effects on growth performance of pigs

We conducted three experiments to determine the apparent ileal digestibility of amino acids (Exp. 1), metabolizable and digestible energy (Exp. 2), and feeding value (Exp. 3) of dry extruded-expelled soybean meal with (DEH) or without (DENH) hulls compared with solvent-extracted soybean meal with hulls removed (SBMNH). Soybeans used to produce DEH were unadulterated prior to extrusion, but those used for DENH were dehulled prior to extrusion. In Exp. 1, six nonlittermate barrows (initially 39 kg) were fitted with ileal T-cannulas and used in a replicated 3 x 3 Latin square design digestion trial. Experimental diets (0.80% total lysine) were cornstarch-based and contained soybean meal from one of the three different sources as the sole source of lysine. Apparent ileal digestibilies of nutrients were similar (P > 0.10) for DEH and DENH. Apparent ileal digestibilies of CP, Lys, Ile, Leu, Arg, Phe, and Val were greater (P < 0.05) for DEH and DENH than for SBMNH. In Exp. 2, six barrows (initially 41 kg) were fed three corn-based diets containing 25% of one of the three soybean meal sources. A fourth diet was fed at the end of the trial containing all ingredients except soybean meal, so that energy values of the soybean meal could be determined by difference. Digestible energy and ME contents were similar (P > 0.10) for DEH and DENH and both had greater (P < 0.05) DE and ME contents than SBMNH. In Exp. 3, pigs (n = 216, initially 10.6 +/- 1.3 kg and 35 +/- 3 d of age) were blocked by weight and allotted to six dietary treatments. Corn-soybean meal-based diets (0.95% digestible lysine and 3.44 kcal/g ME) containing DEH or DENH were compared with similar diets containing SBMNH or solvent-extracted soybean meal with hulls (SBMH). In addition, a diet containing a second expelled soybean meal with hulls (ESBM) was compared with a diet containing SBMH and soy oil. Growth performance of pigs fed diets containing DEH or DENH was not different (P > 0.10) than that of pigs fed corresponding diets containing SMBH or SBMNH. Pigs fed ESBM had lower (P < 0.05) ADG and G/F compared with its corresponding SBMH and soy oil diet. In conclusion, DEH and DENH are more digestible than conventional soybean meal and can be successfully used in swine diets.     

The effect of microbial phytase on true and apparent ileal amino acid digestibilities in growing-finishing pigs

Ten 56-d-old, 15-kg barrows were surgically fitted with a postvalvular T-cecum cannula at the ileo-cecal junction to evaluate the effect of microbial phytase on apparent and true ileal AA digestibility and N utilization. A semipurified cornstarch- and soybean meal-based diet was formulated to contain 3.4 Mcal of DE/kg, 17.0% CP, 0.8% Ca, and 0.6% P but had a low phytate-P concentration (0.13%; all on an as-fed basis). Chromic oxide and dysprosium chloride were used as indigestible markers. The basal diet was supplemented with 0 or 1,000 phytase units/kg of microbial phytase. Postprandial plasma urea N and alpha-amino N concentrations, excretion of Ca, P, and N in feces and urine, and ileal AA digestibilities were determined 3 times at 4-wk intervals beginning at 70 d of age. The homoarginine (HA) method was used to determine endogenous AA flow by replacing 50% of the basal protein with guanidinated protein. Microbial phytase had no effect on apparent ileal digestibility (AID) or on true ileal digestibilities of N and most AA but did increase AID for arginine (P = 0.006) and methionine (P = 0.037). However, in HA diets, phytase increased the AID of CP (P = 0.01) and several AA. Addition of microbial phytase had no effect on the postprandial alpha-amino N concentrations in plasma but increased overall plasma urea N concentrations (P = 0.035). Barrows fed phytase-supplemented diets had decreased P in feces (P = 0.003) and greater P in urine (P = 0.001) but comparable total P excretion compared with barrows fed no phytase-supplemented diets. In conclusion, the addition of phytase to a semi-purified soybean meal-based diet did not affect the AID of several AA. In addition, differences between the basal and HA diets in N digestibilities indicated that that guanidination may limit the use of the HA method in determining endogenous protein losses.     

Effects of level of supplemental phytase on ileal digestibility of amino acids, calcium, and phosphorus in dehulled soybean meal for growing pigs.

Ileally cannulated pigs were used to assess the effects of four dietary levels of microbial phytase (Natuphos) on the apparent and true digestibility of Ca, P, CP, and AA in dehulled soybean meal. Fourteen pigs (25 kg initial BW) were surgically fitted with T-cannulas at the terminal ileum and assigned to diets in a replicated 7 x 7 Latin square design. Following a 14-d recovery, four diets consisting of 30.5% soybean meal with 0, 500, 1,000, or 1,500 units of phytase/kg of diet were fed. Diets 5 (1.05% lysine, 0.90% Ca, and 0.75% P) and 6 (1.05% lysine, 0.90% Ca, and 0.75% P) contained 35.25% soybean meal and 27.0% soy protein concentrate, respectively. Diet 7 (0.37% lysine, 0.03% Ca, and 0.05% P) was a low-CP, casein-based diet used to estimate the nonspecific endogenous losses of Ca, P, CP, and AA in order to estimate the true digestibility of these nutrients. All diets contained cornstarch and dextrose and were fortified with vitamins and minerals. Chromic oxide was used as an indigestible indicator. The diets were fed daily at 9% of metabolic BW (BW0.75). Apparent and true ileal digestibility of P increased quadratically (P < 0.01) and true digestibility of Ca increased linearly (P < 0.07) with increasing levels of phytase. Apparent digestibility of Ca was unaffected (P = 0.15) by phytase level. Apparent and true ileal digestibility of CP and most AA increased slightly with the addition of 500 units of phytase/kg of diet, but not at higher levels of phytase supplementation (in most cases, cubic effect, P < 0.05). Apparent and true ileal nutrient digestibility coefficients were unaffected by soybean meal source (Diet 1 vs Diet 5), except for arginine and Ca. The apparent and true digestibility coefficients for most of the AA tended (P < 0.10) to be lower in diets containing soy protein concentrate vs the common source of soybean meal used in Diet 5, but ileal digestibilities of Ca and P were unaffected (P = 0.15). In this study, supplemental microbial phytase did not improve the utilization of AA provided by soybean meal but was an effective means of improving Ca and P utilization by growing swine fed soybean meal-based diets.     

Ileal digestibility of amino acids,phosphorus, phytate and energy in pigs fed sorghum‐based diets supplemented with phytase and Pancreatin®

The effects of phytase supplementation on the apparent ileal digestibility (AID) of amino acids (AA) have been inconsistent. Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin^®) to growing pigs fed sorghum–soybean meal diets supplemented with phytase on the AID of AA, energy, and phosphorus (P), as well as the ileal digestibility (ID) of phytate; there were four periods per experiment. In Experiment 1, eight pigs (BW 22.1 ± 1.3 kg) were fitted with a T‐cannula at the distal ileum. Each period consisted of 9 days; 7 days for diet adaptation, and 2 days for digesta collection. Treatments (T) were: (i) basal sorghum–soybean meal diet, (ii) basal diet plus Pancreatin^®, (iii) basal diet plus phytase and (iv) basal diet plus phytase and Pancreatin^®. Phytase increased the digestibilities of phytate and P (p < 0.001), but did not affect the AID of AA and energy (p > 0.10). Except for methionine (p = 0.07), Pancreatin^® did not affect the AID of AA. Phytase and Pancreatin^® did not interact (p > 0.10). Experiment 2 was similar to Experiment 1, but Pancreatin^® was infused into duodenum. Pancreatin^® infusion did not affect the AID of AA (p > 0.10); and tended to reduce (p = 0.09) the AID of lysine. Phytase × Pancreatin^® interactions were not observed (p > 0.10). In conclusion, phytase and Pancreatin^® did not improve the AID of AA in growing pigs fed sorghum–soybean meal diets indicating that phytates did not affect AA digestibility.     

Effects of catfish meal blend inclusion in broiler feeds on live performance and carcass yield attributes

This study was designed to investigate whether a catfish meal blend, formulated into broiler feed as a protein supplement, would influence broiler growth and meat yield when included in the diet at industry-acceptable levels. A catfish meal blend was fed at an inclusion rate of 7.5% of the diet to broilers raised to 42 d on a 3-feed program. This treatment was compared with broilers raised on a corn-soybean meal control diet (no animal protein products) and a diet with 7.5% poultry by-product meal. Each animal protein product was formulated into the diets based on a nutritional matrix and was not substituted for another ingredient. The broilers were raised with 50 birds per pen (10 replicate pens per treatment). Five males and 5 females per pen were processed and deboned at 42 d to compare carcass and meat yields. Body weights at 21 d were highest for birds fed the catfish meal blend, with the corn-soybean meal control treatment being the lowest and the poultry by-product meal treatment being intermediate. No significant differences were recorded after 21 d, and no differences were seen in period gains from 21 to 35 or 35 to 42 d. Feed conversion at 21 d favored birds in those treatments fed animal protein products; however, no significant differences were seen at 35 or 42 d. No significant differences were noted in mortality during any of the production periods. Chilled carcass, fillet, and tender yields were not significantly affected by feeding either of the animal protein products. In conclusion, the catfish meal blend included in broiler feeds at an industry-acceptable level (7.5%) showed no adverse effects on broilers grown to 42 d. No health effects were recorded and live performance parameters were not reduced in comparison with the other treatments.     

Interrelationship between hypersensitivity to soybean proteins and growth performance in early-weaned pigs.

The objective of this growth trial was to determine the interrelationship between immunological criteria, gut morphology, and performance of starter pigs fed soybean proteins processed by different methods. One hundred twenty-five pigs were orally infused with 6 g/d of either dried skim milk, soybean meal (48% CP), soy protein concentrate, extruded soy protein concentrate, or experimental soy protein concentrate from 7 to 11 d of age and then fed a diet containing the same protein sources from weaning (d 21) to 35 d of age. All pigs were fed a corn-soybean meal diet containing 10% dried whey, 1.25% lysine, and 3% soybean oil for the remaining 21 d of the experiment. Xylose absorption and anti-soy immunoglobulin G (IgG) titers were measured on d 6 postweaning, and skin-fold thickness after intradermal injection of protein extracts was measured on d 7 postweaning. A total of 25 pigs (five pigs/treatment) was euthanatized on d 7 postweaning. Villus height and crypt depth from duodenum samples were measured. These measurements were obtained to elucidate a relationship between the hypersensitivity responses to soybean products and growth performance of baby pigs. Pigs fed diets containing soybean meal had a lower (P less than .05) rate of gain (d 0 to 14) and villus height, higher (P less than .01) serum anti-soy IgG titers, and increased skin-fold thickness (d 6 and 7 postweaning) after intradermal injection compared with those fed dried skim milk. Pigs fed other soy proteins also had lower ADG from d 0 to 14 postweaning; however, pigs fed moist extruded soy protein concentrate tended (P less than .09) to have higher ADG and improved feed utilization when compared with pigs fed soybean meal (d 0 to 14).(ABSTRACT TRUNCATED AT 250 WORDS)     

Determination of apparent ileal amino acid digestibility in rapeseed meal and cake processed at different temperatures using the direct and difference method with growing pigs.

Studies were conducted with ten barrows, average initial body weight 34.5 +/- 2.1 kg, fitted with a T-cannula at the distal ileum, to study the accuracy of determination of the apparent ileal digestibility (AID) values of crude protein (CP) and amino acids (AA) in rapeseed meal and cake and the effects of processing, using the difference method. Five corn starch-based diets in the studies were formulated to contain 17.7% CP and based on soybean meal, prepress-extraction rapeseed meal, prepress-extraction rapeseed meal plus soybean meal, high-temperature press rapeseed cake plus soybean meal, or low-temperature press rapeseed cake plus soybean meal as the sole source of dietary protein. The design was an incomplete Latin Square involving two three-week periods and five-treatments. It was found that the AID values of CP and most AA determined with the difference or direct method were significantly lower in rapeseed meal or cakes than soybean meal. The AID values of CP and most AA in prepress-extraction rapeseed meal, high-temperature press or low-temperature cakes determined with the difference method were no difference from those in prepress-extraction rapeseed meal determined with the direct method. The AID values of CP and AA in rapeseed meal and cake determined with the difference method were accurate, when the contribution of CP and AA from rapeseed was more than 50%. The AID values of CP and AA (especially lysine) were lower in the high-temperature press rapeseed cake than in the low-temperature press cake or the prepress-extraction meal.