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1.
S. Khériji M. El Cafsi W. Masmoudi J.D. Castell M.S. Romdhane 《Aquaculture International》2003,11(6):571-582
A large part of the extensive aquaculture in Tunisia was based on the transfer of mullet fry from the marine coastal areas
to the continental fresh water lakes, the aim of this work is the monitoring of the changes made in the lipid composition
of Mugil cephalus fry according to salinity and temperature acclimation. Fish acclimated to 14 °C and 0.5 psu salinity, contained 55% less
total fatty acid than those acclimated to the seawater at the same temperature. Low salinity induces an increase in the percentage
of some polyunsaturated fatty acid such as the 22:5 (n-3), the 22:6 (n-3) and the 20:4 (n-6), as a result of change in the level of triacylglycerol content or the activation in the synthesis of those fatty acids.
The transition of the acclimation temperature from 26 to 14 °C in seawater is followed by a 215% increase in the amount of
total fatty acids in the fourth week of acclimation. The total fatty acid levels of all fish at 1 week were higher than most
values at 4 weeks. There was an increase of polyunsaturated fatty acids percentages due to the decrease of the temperature
at the low salinity.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
2.
Malate dehydrogenase isozymes of grass carp,Ctenopharyngodon idella, were identified by mitochondrial preparation and thermal denaturation. The structural and kinetic characteristics of chromatographically separated thermostable and thermolabile cMDHs were different in (1) half-life at 42°C, 10 min and 24 h, respectively, (2) optimal substrate, oxaloacetate and malate, concentrations, and (3) the apparent Michaelis-Menten constants of NADH and oxaloacetate.Total MDH activity in white muscle of 11°C-acclimated fish was about twice that of the 30°C-acclimated group. In addition, the ratio of the thermostable to thermolabile cMDH activity in white muscle of 30°C-acclimated fish was significantly higher than that of 11°C-acclimated fish. These results suggest that temperature acclimation can induce temperature compensation in MDH activity and differential expression of thermostable and thermolabile cMDH isozymes in freshwater fish. 相似文献
3.
The effects of acclimation temperature and acute temperature change on the uptake and metabolism of the procarcinogen benzo[a]pyrene
(BaP) by gill cells of the gulf toadfish, Opsanus beta, were examined. BaP was rapidly accumulated by isolated gill cells and uptake rates were directly proportional to BaP concentration
in the medium (1 to 100 μg/ml). Uptake rates were higher in cells isolated from fish acclimated to 18°C when compared to cells
from 28°C acclimated fish at all incubation temperatures. When cells were exposed to BaP at the respective acclimation temperatures
of the fish, uptake rates were similar (0.14 ± 0.01 at 18°C and 0.12 ± 0.01 μg BaP/s/10 mg cells at 28°C). This finding is
discussed in view of results which showed a partial compensation of membrane fluidity in plasma membranes isolated from fish
from the two acclimation temperatures. At higher incubation temperatures, cells from fish acclimated to 18°C metabolized BaP
at a greater rate than those at 28°C (49.6 ± 1.92 and 43.0 ± 2.24 μg/g/8h, respectively, at 23°C). Low but detectable activities
of common biotransformation enzymes (aryl hydrocarbon hydroxylase, glutathione-S-transferase) and cytochrome P-450 content
were found, however, no significant differences were evident between cells from fish acclimated to different temperatures.
To whom to address correspondence 相似文献
4.
Shmuel Rothbard William L. Shelton Zeev Kulikovsky Israel Rubinshtein Yair Hagani Boaz Moav 《Aquaculture International》1997,5(1):51-64
The mollusc-eating black carp (Mylopharyngodon piceus) has economic and health-care potential for biological control of nuisance aquatic molluscs. The present study investigates
the production of gynogenetic-monosex and triploid-sterile populations of black carp. The goal was to provide a method which
would eliminate unwanted biological and environmental impacts of introducing this exotic species into areas with nuisance
mollusc infestation. Meiotic gynogenesis was induced by inseminating black carp eggs with UV-irradiated (800 Jm−2) sperm of common carp (Cyprinus carpio) or Japanese ornamental (koi) carp. Diploidy was restored through retention of the second polar body (2PB), by shocking activated
eggs at 1–8 min post-fertilization (embryological age of 0.07–0.57τ0, a parameter defined by the cell cycle duration) at 1 min intervals, with heat-shocks (41.0±1.0 °C for 1 min) or pressure-shocks
(7500–7600 psi for 1.5 min). Highest survival was found when embryos were heat-shocked 1.5–4.5 min post-fertilization (0.10–0.25τ0). The highest survival of free-swimming larvae from pressure-shocked eggs, was achieved at 7500 psi at 1–2 min post-fertilization
(0.08–0.16τ0). Triploidy was induced by retention of 2PB following normal fertilization. Batches of 30 000 eggs were fertilized with intact
sperm and pressure-shocked (6000–8500 psi for 1.5 min) 2 min post-fertilization (0.15–0.16τ0). The highest survival of triploid swim-up larvae was 5.1% in eggs shocked with 7500 psi. In random samples of individual
larvae taken from each treatment, triploidy was analysed by cytofluorometry of the cellular DNA content. In DNA analysis performed
in fingerlings (N≥15), 50% of the fish were triploids. 相似文献
5.
Zdeňka Svobodová Jana Máchová Hana Kroupová Miriam Smutná Ladislav Groch 《Aquaculture International》2007,15(3-4):277-286
Three case studies of ammonia autointoxication of the common carp (Cyprinus carpio L.) are described. In the first case, carp yearlings with a full digestive tract were transferred during the growing period
from pond water (22°C) to tap water (17°C). In the second case, marketable carp were transferred from the fishing ground of
a pond (18°C) to a storage pond (10–12°C). Harvest was performed in late September when the fish were still ingesting natural
feed. In the third case, marketable carp after storage for 1 month were transferred to storage ponds with markedly lower water
temperature. Stress because of harvest and handling also occurred in this case. In all cases, highly increased concentrations
of ammonia were found in the blood plasma of the fish (mean ± SD 1760 ± 350 μmol L−1 in the first case, 870 ± 540 μmol L−1 in the second case, and 880 ± 150 μmol L−1 in the third case). Highly congested, dark-red coloured, oedematous gills were observed for all specimens. We can avoid similar
cases of ammonia autointoxication by protection of fish from sudden changes of temperature during rearing, harvesting, and
handling. 相似文献
6.
Respiratory parameters of grass carp were studied during dissolved oxygen (DO) changes from normal DO to hypoxia, then return to normal DO at 15, 25, and 30 °C acclimation, respectively. The results showed that with increases of acclimation temperature at normoxia the respiratory frequency (fR), oxygen consumption rate (VO2), respiratory stroke volume (VS.R), gill ventilation (VG), and VG/VO2 of grass carp increased significantly, but the oxygen extraction efficiency (EO2) of fish decreased significantly (P < 0.05). With declines of DO levels, the fR, VS.R, VG, and VG/VO2 of fish increased significantly at different acclimation temperatures (P < 0.05). A slight increase was found in VO2, and the EO2 of fish remained almost constant above DO levels of 3.09, 2.91, and 2.54 mg l?1 at 15, 25, and 30 °C, while the VO2 and EO2 began to decrease significantly with further reductions in DO levels (P < 0.05). After 0.5 h of recovery to normoxia from hypoxia at three acclimation, the fR, VS.R, VG, and VG/VO2 of the fish decreased sharply; meanwhile, the VO2 and EO2 increased sharply (P < 0.05). The respiratory parameters of fish gradually approached initial values with prolonged recovery time to normoxia, and reached their initial values in 2.5 h at 25 and 30 °C acclimation. The critical oxygen concentrations (Cc) of fish for VO2 were 2.42 mg l?1 at 15 °C, 2.02 mg l?1 at 25 °C, and 1.84 mg l?1 at 30 °C, respectively. The results suggest that grass carp are highly adapted to varied DO and short-term hypoxia environments. 相似文献
7.
Critical thermal minima (CTMin) were determined for subadults of Penaeus merguiensis acclimated at 15, 18, 21 and 24 °C for 3 weeks. The effect of cooling rate on CTMin was also investigated. The CTMin of prawns from these respective acclimation groups were 5.3, 6.0, 7.4, 9.0 and 4.7, 5.4, 6.0, 7.3 °C at the cooling rate of 1 °C h?1 and 3 °C h?1 respectively. Both acclimation temperature and cooling rate had a significant effect (P < 0.01) on the CTMin. Observation during the acclimation period showed that the growth rate of prawns acclimated at 24 °C was much higher than those acclimated at 21, 18 and 15 °C. The results indicate that P. merguiensis can overwinter in aquaculture ponds in south‐east Queensland, Australia, or other subtropical areas and attain good growth if simple overwintering facilities are available. 相似文献
8.
Supplemental cereal feeding (maize, wheat and triticale compared with a control group with natural food only) and its effect
on fatty acid (FA) expression in the flesh during long-lasting storage of common carp (Cyprinus carpio) was investigated. The fish were cultured in earthen ponds in the Trebon region (Czech Republic). The content of fatty acid
was investigated in the flesh of carp during 8 months of long-lasting storage without additional feeding. Sixty common carp
in their third year of life were used for the analyses. The weight of the fish (marketable fish) ranged from 1,358 g to 2,221 g.
Polyunsaturated fatty acid (PUFA, n–3) content and composition in fish flesh were determined by gas chromatography (VARIAN 3300). Supplemental cereals caused
lower levels of PUFAs and n–3 PUFAs in fish fat. The content of these fatty acids did not decrease, even during 8 months of fish storage. The average
percentage of PUFAs in total fat from edible parts was: for maize 13.7% ± 1.58%, for wheat 11.6% ± 1.17% and for triticale
10.7% ± 1.00%. The percentage of n–3 PUFA for maize was 2.5% ± 0.36%, for wheat 3.38% ± 0.44% and for triticale 3.1% ± 0.39%. 相似文献
9.
In this study, we examine changes in fatty acid composition of polar and neutral lipids in gill, liver, and muscle of freshwater
alewives (Alosa pseudoharengus) after temperature challenges in the laboratory. Alewives experienced either a warm or cold challenge in which temperatures
were raised or lowered 0.5°C day−1 over 4–6 weeks. In alewives experiencing the cold challenge, gill polar lipids showed evidence of significant remodeling,
including decreases in palmitic acid and saturated fatty acids and increases in n-3 and n-6 highly unsaturated fatty acids including docosahexaenoic acid (DHA) and arachidonic acid. In alewives experiencing the
warm challenge, we observed significant increases in saturated fatty acids (due mainly to increased palmitic acid) and decreases
in polyunsaturated fatty acids in polar lipids of muscle and liver tissue. Fish that died during the cold challenge had significantly
higher levels of palmitic acid in muscle polar lipids compared to fish that survived; fish that died during the warm challenge
displayed complex changes in fatty acid composition. Based on theoretical considerations, the changes in polar lipids we observed
during thermal acclimation are likely to promote appropriate membrane fluidity under each thermal regime. The increased incorporation
of highly unsaturated fatty acids during cold acclimation could have significant physiological and ecological implications.
In particular, since highly unsaturated fatty acids are typically scarce in freshwater food webs, dietary deficiencies in
these essential fatty acids may be a significant factor in winter mortality of freshwater alewives. 相似文献
10.
Cold-active burbot (Lota lota (L.)) display reduced food intake during the summer. The impact of temperature on their energy budget was investigated in
starved fish in a laboratory setting, simulating summer (20°C) and winter (4°C) conditions, to elucidate the impact of high
temperature on burbot metabolism. Metabolic effects in burbot were compared to roach (Rutilus rutilus (L.)), which typically fast in winter. During warm acclimation, starvation (four weeks) resulted in a metabolic depression
of oxygen consumption in both species. In roach, metabolic rate decreased by 55% after two weeks of starvation. Burbot, in
contrast, displayed an immediate depression of metabolic rate by 50%. In both species, no reductions were observed in the
cold. The temperature-induced differences between the metabolic rates at 20°C and 4°C showed a lower thermal sensitivity in
burbot (Q
10 = 1.9) compared to roach (Q
10 = 2.7). Notably, for each species, energy consumption during starvation was highest under experimental conditions simulating
their natural active periods, respectively. Warm acclimated roach relied mainly on muscle reserves, whereas in cold acclimated
burbot, liver metabolic stores made a major contribution to the energy turnover. In cold acclimated roach and warm acclimated
burbot, however, starvation apparently reduced swimming activity, resulting in considerable savings of energy reserves. These
lower energy expenditures in roach and burbot corresponded to their natural inactive periods. Thus, starvation in burbot caused
a lower energy turnover when exposed to high temperatures. These season-dependent adaptations of metabolism represent an advantageous
strategy in burbot to manage winter temperature and withstand metabolism-activating summer temperatures, whereas roach metabolism
correlates with the seasonal temperature cycle. 相似文献
11.
Photosynthetic activities of seedlings of Zostera marina were successively measured using a gas volumeter for 6 days at seven light (0–400 μmol photons/m2 per s) and 11 water temperature conditions (5–35°C). The seedlings were collected from mature plants (Ise Bay, central Japan),
and stored and cultured in incubators accurately controlled at each test temperature. The maximum gross photosynthesis (P
maxg) was recorded at an optimal water temperature of 29°C after 0 days. After 6 days, P
maxg appeared at 25°C and most plants cultured at 29–30°C bleached and withered after the drastic increase of light compensation
point (I
c). On the contrary, at 5–28°C, the photosynthetic activities either changed little (5–25°C) or recovered after a temporal
reduction (26–28°C); seedlings survived and looked healthy after being cultured for 6 days. The recovery was thought to be
an acclimation to tolerate higher water temperature. As a result, the critical upper water temperature for Z. marina seedlings was proposed as 28°C. The temperature was consistent with the previously reported maximum water temperature in
habitats around the southern boundary of Z. marina in the northern hemisphere. 相似文献
12.
基于微卫星评估草鱼放流亲本对野生群体遗传多样性的影响 总被引:3,自引:2,他引:1
利用筛选的13对草鱼多态性微卫星标记,开展了2011至2015年长江中游草鱼亲本增殖放流对野生群体遗传多样性的影响评估。通过对各位点的遗传多样性分析,13个微卫星位点的多态信息含量为0.8622(0.657~0.950),基因多样度为0.8555(0.675~0.936)。15个群体的有效等位基因数为7.4503~10.1536,等位基因丰度为11.483~15.204,说明15个草鱼群体的遗传多样性水平总体较高。遗传分化指数分析表明,群体间不存在显著遗传分化(FST5%)。通过贝叶斯聚类分析和主成分分析可将草鱼群体分为4个组群,根据分组结果以及来源划分分别对草鱼群体进行AMOVA分析,发现遗传变异大部分来自于群体内个体间,组间及组内群体间的分化水平较低(FCT5%,FSC5%),与FST分析结果一致。研究表明,当前草鱼亲本增殖放流模式对野生群体遗传结构影响不明显。 相似文献
13.
Dr. Jeffrey R. Hazel 《Fish physiology and biochemistry》1996,15(3):195-204
Most previous studies of the temperature-induced restructuring of phospholipid molecular species composition have examined steps in the biosynthesis of phospholipids to explain the accumulation of unsaturated fatty acids in membranes of cold-acclimated poikilotherms. In contrast, the present study explores the role of phospholipases in this restructuring process by determining the rates of degradation of specific molecular species of phosphatidylcholine, using enzymes (microsomes) freshly isolated from the liver of rainbow trout. (Oncorhynchus mykiss) acclimated to either 5° or 20°C. The substrate preparation employed to assay phospholipase activity possessed a range of molecular species, all radiolabeled with 1-14C-palmitic acid at thesn-1 position, similar to that present in native trout liver microsomes. After defined periods of incubation (120 and 240 min at 5°C; 60 and 120 min at 20°C), phospholipids were extracted from the reaction mixture and the distribution of radioactivity among the molecular species of phosphatidylcholine was determined by HPLC/liquid scintillation counting. In general, molecular species catabolism was not significantly influenced by either assay or acclimation temperature. Only in 20°C-acclimated fish did a reduction in assay temperature (from to 20 to 5°C) result in significantly increased proportions of radioactivity being recovered in one polyunsaturated fatty acid-containing species (16:0/22:6-PC). It is concluded: 1) that phospholipase specificity, assayed under conditions approximating thosein situ, is not significantly influenced by temperature; and 2), that the increased proportions of unsaturated fatty acid-containing molecular species of phosphatidylcholine observed at low temperatures must reflect the specificity of biosynthetic rather than degradative processes. 相似文献
14.
Male ninespine sticklebacks, Pungitius pungitius, acclimated to 3°C have higher activities of mitochondrial enzymes in their axial muscles than males acclimated to 20°C.
Phosphofructokinase and pyruvate kinase activities tended to be higher in cold than warm acclimated males. For females, warm
acclimation tended to decrease only mitochondrial enzyme activities. As thermal acclimation did not change the physical condition
and most anatomic parameters of the sticklebacks, the enzymatic changes do not seem due to mobilization of somatic reserves.
Field acclimatization to warm temperatures led to a marked decrease in physical condition in both males and females. This
decrease in physical condition could largely be attributed to atrophy of the carcass mass. Spring males had higher activities
of phosphofructokinase, citrate synthase and cytochrome oxidase in the axial muscle than summer males. Again, females showed
a less marked response. These data suggest that environmental temperature is a major determinant of muscle aerobic capacity,
at least for male ninespine sticklebacks. Thus, these northern temperate zone fish retain the capacity for thermal compensation,
much like their temperate zone counterparts. 相似文献
15.
Ayşe Gürel Inanlı Özlem Emir Çoban Ökkeş Yılmaz Emine Özpolat Nermin Karaton Kuzgun 《Journal Of Aquatic Food Product Technology》2018,27(7):803-810
In this study, roes obtained from carp (Cyprinus carpio carpio) that were caught in Keban Dam Lake (Turkey) and cultured rainbow trout (Oncorhynchus mykiss) were processed as caviar, and the products were stored at + 4 C°. Lipid peroxidation and the compositions of fatty acids of these products were assessed during production and storage period. The result of fatty analyses showed that palmitic acid (16:0), stearic acid (18:0), palmitoleic acid (16:1), oleic acid (18:1), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), which are all essential fatty acids, were determined in the caviars of the roes obtained from rainbow trout and carp. According to results of chemical analyses, there were no significant differences in thiobarbituric acid reactive substances (TBARs) between rainbow trout and carp caviars (p > 0.05), but malondialdehyde (MDA) levels were found to be statistically significant (p < 0.01). It can be concluded that caviars obtained from both rainbow trout and carp roes are rich in fatty acids and take a part in a balanced and healthy diet. 相似文献
16.
V. Ventrella A. Pagliarani M. Pirini G. Trigari F. Trombetti A. R. Borgatti 《Fish physiology and biochemistry》1993,12(4):293-304
The response to cold of gill and kidney membrane lipid composition and microsomal (Na++K+)-ATPase, Na+-ATPase and Mg2+-ATPase activities in reared sea bass (Dicentrarchus labrax L.) was investigated. Fish acclimation was carried out according to the seasonal cycle from August to March. No cold-promoted
increase in fatty acid unsaturation was shown in gill and kidney polar lipids and in total lipids of mitochondria and microsomes.
In both tissues the (Na++K+)-ATPase exhibited positive compensation for cold acclimation whereas the Na+-ATPase displayed negative compensation. The Mg2+-ATPase showed no compensation in the gills and positive compensation in the kidneys. During cold acclimation the break in
the Arrhenius plot of the (Na++K+)-ATPase decreased, whereas breaks of both the Na+-ATPase and the Mg2+-ATPase activities remained unchanged. The results indicate that the sea bass does not adopt membrane unsaturation as a cold-facing
strategy. The cold-promoted enhancement of (Na++K+)-ATPase activity in osmoregulatory tissues may be advantageous to maintain efficient osmoregulation under thermodynamically
unfavourable conditions. 相似文献
17.
Y. Durmaz M. Donato M. Monteiro L. Gouveia M. L. Nunes T. Gama Pereira Ş. Gökpınar N. M. Bandarra 《Aquaculture International》2009,17(4):391-399
Diacronema vlkianum was grown in polyethylene bags at two different temperatures (18 and 26°C) in the laboratory. The biochemical composition
level decreased when the temperature increased from 18 to 26°C. The maximum cell number at 18°C was 11.9 × 106 cells ml−1, while maximum cell number at 26°C was 1.6 × 106 cells ml−1. The maximum level of α-tocopherol was 257.7 ± 21.6 μg g−1 dry weight (DW) at 18°C. The highest total carotenoids and chlorophylls were 6.5 mg g−1 DW and 4.3 mg g−1 DW, respectively, and the main pigments were determined as astaxanthin and lutein. Polyunsaturated fatty acids were found
to be the predominant group, reaching 39.5% of the total fatty acids at 18°C. This comprised 20:5(n − 3) as the main polyunsaturated
fatty acids (20.4%, at 18°C) followed by 22:6(n − 3) (4.8%, at 18°C). The results suggest that D. vlkianum can be successfully used as feed in shellfish hatcheries or aquaculture hatcheries, either as a substitute or in association
with other microalgae, when this algae is cultured at 18°C. 相似文献
18.
Ling-Qing Zeng Yao-Guang Zhang Zhen-Dong Cao Shi-Jian Fu 《Fish physiology and biochemistry》2010,36(4):1243-1252
The effects of temperature on resting oxygen consumption rate (MO2rest) and excess post-exercise oxygen consumption (EPOC) after exhaustive exercise (chasing) were measured in juvenile southern
catfish (Silurus meridionalis) (8.40 ± 0.30 g, n = 40) to test whether temperature has a significant influence on MO2rest, maximum post-exercise oxygen consumption rate (MO2peak) and EPOC and to investigate how metabolic scope (MS: MO2peak − MO2rest) varies with acclimation temperature. The MO2rest increased from 64.7 (10°C) to 160.3 mg O2 h−1 kg−1 (25°C) (P < 0.05) and reached a plateau between 25 and 30°C. The post-exercise MO2 in all temperature groups increased immediately to the peak values and then decreased slowly to a steady state that was higher
than the pre-exercise MO2. The MO2peak did not significantly differ among the 20, 25 and 30°C groups, though these values were much higher than those of the lower
temperature groups (10 and 15°C) (P < 0.05). The duration of EPOC varied from 32.9 min at 10°C to 345 min at 20°C, depending on the acclimation temperatures.
The MS values of the lower temperature groups (10 and 15°C) were significantly smaller than those of the higher temperature
groups (20, 25 and 30°C) (P < 0.05). The magnitude of EPOC varied ninefold among all of the temperature groups and was the largest for the 20°C temperature
group (about 422.4 mg O2 kg−1). These results suggested that (1) the acclimation temperature had a significant effect on maintenance metabolism (as indicated
by MO2rest) and the post-exercise metabolic recovery process (as indicated by MO2peak, duration and magnitude of EPOC), and (2) the change of the MS as a function of acclimation temperature in juvenile southern
catfish might be related to their high degree of physiological flexibility, which allows them to adapt to changes in environmental
conditions in their habitat in the Yangtze River and the Jialing River. 相似文献
19.
The effects of temperature on respiration of Amur sturgeon,Acipenser schrenckii,at two acclimation temperatures 下载免费PDF全文
Zhigang Zhao Liang Luo Chang'an Wang Jinnan Li Liansheng Wang Xue Du Qiyou Xu 《Aquaculture Research》2017,48(10):5338-5345
In order to clarify the respiratory responses strategy of Amur sturgeon Acipenser schrenckii exposed to water temperature changes, respiratory parameters of the fish were studied under two temperature regimes: fish acclimated at 13°C for Group I, temperature was increased to 16°C, 19°C, 22°C and 25°C and then returned stepwise to 22°C, 19°C, 16°C and 13°C; and fish acclimated at 25°C for Group II, the water temperature was reduced in steps to 22°C, 19°C, 16°C and 13°C, subsequently, returned to 16°C, 19°C, 22°C and 25°C. The results showed that the respiratory frequency (fR), oxygen consumption rate (VO2) and gill ventilation (VG) of the fish were directly dependent on the acute temperature in both acclimation groups (p < .05). The initial 25°C VO2 in Group II was significantly higher than the initial 13°C VO2 in Group I (p < .05), but was significantly lower than that at 25°C in Group I (p < .05). In Group I, respiratory stroke volume (VS.R) of fish significantly increased or decreased with the acute temperature increases or decreases, respectively (p < .05); oxygen consumption efficiencies (EO2) of fish did not significantly show differences when temperature increased to 25°C from 13°C (p > .05), but the EO2 significantly declined while returning to acclimation temperature (p < .05). In Group II, the VS.R of the fish did not significantly change with acute temperature fluctuations between 25 and 13°C (p > .05), while the EO2 increased with acute temperature increases (p < .05). The Q10 values for fR, VO2, VS.R, VG and EO2 were 1.53–1.72, 1.92–2.06, 1.07–1.60, 1.78–2.44 and 1.11–1.65 at 13–25°C of temperature interval respectively. Amur sturgeon showed partial metabolic compensation to temperature changes. The study results suggest that the ability of Amur sturgeon to regulate metabolism in response to acute temperature changes makes this species good adaptability in the aquaculture rearing. 相似文献
20.
We determined full-length cDNA of carp warm-temperature-acclimation-associated 65-kDa protein (Wap65). It encoded 439 amino acid residues with a signal peptide of 22 residues and showed an amino acid sequence identity of 88% to that of goldfish reported before (J. Biol. Chem. 1995. 270: 17087–17092). The number of potential N-linked glycosylation sites of carp Wap65 was two in contrast to three for goldfish. In addition, molecular mass determined by SDS-PAGE was apparently different from that of goldfish. These results suggest that the amount of oligosaccharide is different between the carp and goldfish protein. As in goldfish, carp Wap65 mRNA showed marked accumulation in hepatopancreas of the 30 °C- acclimated fish, which was 8-fold higher than that of the 10 °C-acclimated fish. Carp Wap65 showed 30% amino acid identity to mammalian hemopexins, which appeared to be considerably low in comparison with those among mammalian hemopexins (72 to 80%), or among carp Wap65 and rainbow trout hemopexin-like protein (70%). However, although mammalian hemopexins contain residues comprising the heme binding pocket, carp Wap65 lacked one of the two histidine residues to serve as heme axial ligands in hemopexins. Our data on carp protein substantiates the previous observation for goldfish and indicates that Wap65 might have some important functions in warm-temperature-acclimation of fish. 相似文献